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P34345

- GSTO1_CAEEL

UniProt

P34345 - GSTO1_CAEEL

Protein

Glutathione transferase omega-1

Gene

gsto-1

Organism
Caenorhabditis elegans
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 107 (01 Oct 2014)
      Sequence version 1 (01 Feb 1994)
      Previous versions | rss
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    Functioni

    Exhibits glutathione-dependent thiol transferase activity. Has dehydroascorbate reductase activity and may contribute to the recycling of ascorbic acid. Participates in the biotransformation of inorganic arsenic and reduces monomethylarsonic acid (MMA) By similarity. Protects against environmental stress and oxidative stress.By similarity1 Publication

    Catalytic activityi

    RX + glutathione = HX + R-S-glutathione.1 Publication
    2 glutathione + dehydroascorbate = glutathione disulfide + ascorbate.1 Publication
    Methylarsonate + 2 glutathione = methylarsonite + glutathione disulfide + H2O.1 Publication

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei33 – 331NucleophileBy similarity
    Binding sitei60 – 601GlutathioneBy similarity

    GO - Molecular functioni

    1. glutathione dehydrogenase (ascorbate) activity Source: UniProtKB
    2. glutathione transferase activity Source: UniProtKB
    3. methylarsonate reductase activity Source: UniProtKB-EC

    GO - Biological processi

    1. cellular response to extracellular stimulus Source: UniProtKB
    2. oxidation-reduction process Source: WormBase
    3. protein glutathionylation Source: UniProtKB
    4. response to oxidative stress Source: UniProtKB
    5. response to superoxide Source: WormBase

    Keywords - Molecular functioni

    Oxidoreductase, Transferase

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Glutathione transferase omega-1 (EC:2.5.1.18)
    Alternative name(s):
    Glutathione-dependent dehydroascorbate reductase (EC:1.8.5.1)
    Monomethylarsonic acid reductase (EC:1.20.4.2)
    Short name:
    MMA(V) reductase
    Gene namesi
    Name:gsto-1
    ORF Names:C29E4.7
    OrganismiCaenorhabditis elegans
    Taxonomic identifieri6239 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis
    ProteomesiUP000001940: Chromosome III

    Organism-specific databases

    WormBaseiC29E4.7; CE00089; WBGene00016204; gsto-1.

    Subcellular locationi

    Cytoplasm By similarity

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 250250Glutathione transferase omega-1PRO_0000185889Add
    BLAST

    Proteomic databases

    PaxDbiP34345.
    PRIDEiP34345.

    Expressioni

    Tissue specificityi

    Expressed in the intestinal cells.1 Publication

    Interactioni

    Subunit structurei

    Homodimer.By similarity

    Protein-protein interaction databases

    BioGridi47837. 1 interaction.
    DIPiDIP-24588N.
    MINTiMINT-1080999.
    STRINGi6239.C29E4.7.

    Structurei

    3D structure databases

    ProteinModelPortaliP34345.
    SMRiP34345. Positions 7-250.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini21 – 10181GST N-terminalAdd
    BLAST
    Domaini106 – 234129GST C-terminalAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni85 – 862Glutathione bindingBy similarity

    Sequence similaritiesi

    Belongs to the GST superfamily. Omega family.Curated
    Contains 1 GST C-terminal domain.Curated
    Contains 1 GST N-terminal domain.Curated

    Phylogenomic databases

    eggNOGiNOG288793.
    GeneTreeiENSGT00390000005479.
    HOGENOMiHOG000006560.
    InParanoidiP34345.
    KOiK00799.
    OMAiHYQGKAP.
    OrthoDBiEOG71CFNG.
    PhylomeDBiP34345.

    Family and domain databases

    Gene3Di1.20.1050.10. 1 hit.
    3.40.30.10. 1 hit.
    InterProiIPR010987. Glutathione-S-Trfase_C-like.
    IPR004045. Glutathione_S-Trfase_N.
    IPR004046. GST_C.
    IPR005442. GST_omega.
    IPR012336. Thioredoxin-like_fold.
    [Graphical view]
    PfamiPF00043. GST_C. 1 hit.
    PF02798. GST_N. 1 hit.
    [Graphical view]
    PRINTSiPR01625. GSTRNSFRASEO.
    SUPFAMiSSF47616. SSF47616. 1 hit.
    SSF52833. SSF52833. 1 hit.
    PROSITEiPS50405. GST_CTER. 1 hit.
    PS50404. GST_NTER. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P34345-1 [UniParc]FASTAAdd to Basket

    « Hide

    MVLTGVTSKA IRKGDAEPPL SKGSFRVYNM RFCPWAERAM LYVAAKGIEA    50
    EVVNLNVTDK LEWYWTKHYQ GKAPAVEHNG KVVIESGFIP EYLDDAFPET 100
    RILPTDPYEK VQQKLLADRL TAVAHAVPLL FAVMRDRTLK DEKQRKVFEV 150
    LKQAENLLAN DFYAGSQPGY PDYLSFPFFE KIWWSASLDG VVDLPTIEFP 200
    GEEEYPKLTK WFQKMISSDV VQSVTQSLEH GAAFMNAYAT HQELNYDLGL 250
    Length:250
    Mass (Da):28,478
    Last modified:February 1, 1994 - v1
    Checksum:i4EB0E85351217C55
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    FO080706 Genomic DNA. Translation: CCD66006.1.
    PIRiS44768.
    RefSeqiNP_498728.1. NM_066327.1.
    UniGeneiCel.10781.

    Genome annotation databases

    EnsemblMetazoaiC29E4.7; C29E4.7; WBGene00016204.
    GeneIDi183000.
    KEGGicel:CELE_C29E4.7.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    FO080706 Genomic DNA. Translation: CCD66006.1 .
    PIRi S44768.
    RefSeqi NP_498728.1. NM_066327.1.
    UniGenei Cel.10781.

    3D structure databases

    ProteinModelPortali P34345.
    SMRi P34345. Positions 7-250.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 47837. 1 interaction.
    DIPi DIP-24588N.
    MINTi MINT-1080999.
    STRINGi 6239.C29E4.7.

    Proteomic databases

    PaxDbi P34345.
    PRIDEi P34345.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblMetazoai C29E4.7 ; C29E4.7 ; WBGene00016204 .
    GeneIDi 183000.
    KEGGi cel:CELE_C29E4.7.

    Organism-specific databases

    CTDi 183000.
    WormBasei C29E4.7 ; CE00089 ; WBGene00016204 ; gsto-1.

    Phylogenomic databases

    eggNOGi NOG288793.
    GeneTreei ENSGT00390000005479.
    HOGENOMi HOG000006560.
    InParanoidi P34345.
    KOi K00799.
    OMAi HYQGKAP.
    OrthoDBi EOG71CFNG.
    PhylomeDBi P34345.

    Miscellaneous databases

    NextBioi 919570.

    Family and domain databases

    Gene3Di 1.20.1050.10. 1 hit.
    3.40.30.10. 1 hit.
    InterProi IPR010987. Glutathione-S-Trfase_C-like.
    IPR004045. Glutathione_S-Trfase_N.
    IPR004046. GST_C.
    IPR005442. GST_omega.
    IPR012336. Thioredoxin-like_fold.
    [Graphical view ]
    Pfami PF00043. GST_C. 1 hit.
    PF02798. GST_N. 1 hit.
    [Graphical view ]
    PRINTSi PR01625. GSTRNSFRASEO.
    SUPFAMi SSF47616. SSF47616. 1 hit.
    SSF52833. SSF52833. 1 hit.
    PROSITEi PS50405. GST_CTER. 1 hit.
    PS50404. GST_NTER. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Oxidative stress in Caenorhabditis elegans: protective effects of the Omega class glutathione transferase (GSTO-1)."
      Burmeister C., Luersen K., Heinick A., Hussein A., Domagalski M., Walter R.D., Liebau E.
      FASEB J. 22:343-354(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, CATALYTIC ACTIVITY, TISSUE SPECIFICITY.
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: Bristol N2.
    3. "Genome sequence of the nematode C. elegans: a platform for investigating biology."
      The C. elegans sequencing consortium
      Science 282:2012-2018(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: Bristol N2.

    Entry informationi

    Entry nameiGSTO1_CAEEL
    AccessioniPrimary (citable) accession number: P34345
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1994
    Last sequence update: February 1, 1994
    Last modified: October 1, 2014
    This is version 107 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programCaenorhabditis annotation project

    Miscellaneousi

    Miscellaneous

    Worms lacking gsto-1 exhibit increased sensitivity to heat shock and oxidative stress induced by several prooxidants and arsenite.

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Caenorhabditis elegans
      Caenorhabditis elegans: entries, gene names and cross-references to WormBase
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3