Glutathione transferase omega-1 - Caenorhabditis elegans

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P34345 (GSTO1_CAEEL) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 96. History...

Clusters with 100%, 90%, 50% identity |

Documents (2) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Glutathione transferase omega-1
EC=2.5.1.18

Alternative name(s):
Glutathione-dependent dehydroascorbate reductase
EC=1.8.5.1
Monomethylarsonic acid reductase
Short name=MMA(V) reductase
EC=1.20.4.2

Gene names

Name:	gsto-1
ORF Names:	C29E4.7

Organism

Caenorhabditis elegans [Reference proteome]

Taxonomic identifier

6239 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Ecdysozoa › Nematoda › Chromadorea › Rhabditida › Rhabditoidea › Rhabditidae › Peloderinae › Caenorhabditis

Protein attributes

Sequence length	250 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Exhibits glutathione-dependent thiol transferase activity. Has dehydroascorbate reductase activity and may contribute to the recycling of ascorbic acid. Participates in the biotransformation of inorganic arsenic and reduces monomethylarsonic acid (MMA) By similarity. Protects against environmental stress and oxidative stress. Ref.1
Catalytic activity	RX + glutathione = HX + R-S-glutathione. Ref.1 2 glutathione + dehydroascorbate = glutathione disulfide + ascorbate. Ref.1 Methylarsonate + 2 glutathione = methylarsonite + glutathione disulfide + H₂O. Ref.1
Subunit structure	Homodimer By similarity.
Subcellular location	Cytoplasm By similarity.
Tissue specificity	Expressed in the intestinal cells. Ref.1
Miscellaneous	Worms lacking gsto-1 exhibit increased sensitivity to heat shock and oxidative stress induced by several prooxidants and arsenite.
Sequence similarities	Belongs to the GST superfamily. Omega family. Contains 1 GST C-terminal domain. Contains 1 GST N-terminal domain.

Ontologies

Keywords
Cellular component	Cytoplasm
Molecular function	Oxidoreductase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cellular response to extracellular stimulus Inferred from direct assay Ref.1. Source: UniProtKB protein glutathionylation Inferred from direct assay Ref.1. Source: UniProtKB response to oxidative stress Inferred from direct assay Ref.1. Source: UniProtKB
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	glutathione dehydrogenase (ascorbate) activity Inferred from direct assay Ref.1. Source: UniProtKB glutathione transferase activity Inferred from direct assay Ref.1. Source: UniProtKB methylarsonate reductase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 250

250

Glutathione transferase omega-1

PRO_0000185889

Regions

Domain

21 – 101

GST N-terminal

Domain

106 – 234

129

GST C-terminal

Region

85 – 86

Glutathione binding By similarity

Sites

Active site

Nucleophile By similarity

Binding site

Glutathione By similarity

Sequences

Sequence

Length

Mass (Da)

Tools

P34345 [UniParc].

Last modified February 1, 1994. Version 1.
Checksum: 4EB0E85351217C55
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FASTA

250

28,478

        10         20         30         40         50         60 
MVLTGVTSKA IRKGDAEPPL SKGSFRVYNM RFCPWAERAM LYVAAKGIEA EVVNLNVTDK 

        70         80         90        100        110        120 
LEWYWTKHYQ GKAPAVEHNG KVVIESGFIP EYLDDAFPET RILPTDPYEK VQQKLLADRL 

       130        140        150        160        170        180 
TAVAHAVPLL FAVMRDRTLK DEKQRKVFEV LKQAENLLAN DFYAGSQPGY PDYLSFPFFE 

       190        200        210        220        230        240 
KIWWSASLDG VVDLPTIEFP GEEEYPKLTK WFQKMISSDV VQSVTQSLEH GAAFMNAYAT 

       250 
HQELNYDLGL

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Oxidative stress in Caenorhabditis elegans: protective effects of the Omega class glutathione transferase (GSTO-1)." Burmeister C., Luersen K., Heinick A., Hussein A., Domagalski M., Walter R.D., Liebau E. FASEB J. 22:343-354(2008) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, CATALYTIC ACTIVITY, TISSUE SPECIFICITY.
[2]	"2.2 Mb of contiguous nucleotide sequence from chromosome III of C. elegans." Wilson R., Ainscough R., Anderson K., Baynes C., Berks M., Bonfield J., Burton J., Connell M., Copsey T., Cooper J., Coulson A., Craxton M., Dear S., Du Z., Durbin R., Favello A., Fraser A., Fulton L. Wohldman P. Nature 368:32-38(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Bristol N2.
[3]	"Genome sequence of the nematode C. elegans: a platform for investigating biology." The C. elegans sequencing consortium Science 282:2012-2018(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Bristol N2.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	FO080706 Genomic DNA. Translation: CCD66006.1.
PIR	S44768.
RefSeq	NP_498728.1. NM_066327.1.
UniGene	Cel.10781.
3D structure databases
ProteinModelPortal	P34345.
SMR	P34345. Positions 7-250.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-24588N.
MINT	MINT-1080999.
STRING	6239.C29E4.7.
Proteomic databases
PaxDb	P34345.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblMetazoa	C29E4.7; C29E4.7; C29E4.7.
GeneID	183000.
KEGG	cel:CELE_C29E4.7.
Organism-specific databases
CTD	183000.
WormBase	C29E4.7; CE00089; WBGene00016204; gsto-1.
Phylogenomic databases
eggNOG	NOG288793.
GeneTree	ENSGT00390000005479.
HOGENOM	HOG000006560.
InParanoid	P34345.
KO	K00799.
OMA	PAVEHNG.
Family and domain databases
Gene3D	1.20.1050.10. 1 hit. 3.40.30.10. 1 hit.
InterPro	IPR010987. Glutathione-S-Trfase_C-like. IPR004045. Glutathione_S-Trfase_N. IPR017933. Glutathione_S_Trfase/Cl_chnl_C. IPR004046. GST_C. IPR005442. GST_omega. IPR012336. Thioredoxin-like_fold. [Graphical view]
Pfam	PF00043. GST_C. 1 hit. PF02798. GST_N. 1 hit. [Graphical view]
PRINTS	PR01625. GSTRNSFRASEO.
SUPFAM	SSF47616. GST_C_like. 1 hit. SSF52833. Thiordxn-like_fd. 1 hit.
PROSITE	PS50405. GST_CTER. 1 hit. PS50404. GST_NTER. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	919570.

Entry information

Entry name

GSTO1_CAEEL

Accession

Primary (citable) accession number: P34345

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1994
Last sequence update:	February 1, 1994
Last modified:	May 1, 2013
This is version 96 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Caenorhabditis annotation project

Relevant documents

Caenorhabditis elegans

Caenorhabditis elegans: entries, gene names and cross-references to WormBase

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Sites

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Family and domain databases

Other

Entry information

Relevant documents