Reviewed, UniProtKB/Swiss-Prot P34329 (PDIA4_CAEEL)

Names and origin

Protein names

Recommended name:
    Probable protein disulfide-isomerase A4
    EC=5.3.4.1
Alternative name(s):
    ERp-72 homolog

Gene names

ORF Names:

C14B9.2

Organism

Caenorhabditis elegans [Complete proteome]

Taxonomic identifier

6239 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Nematoda › Chromadorea › Rhabditida › Rhabditoidea › Rhabditidae › Peloderinae › Caenorhabditis

Protein attributes

Sequence length	618 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Catalytic activity	Catalyzes the rearrangement of -S-S- bonds in proteins.
Subcellular location	Endoplasmic reticulum lumen By similarity.
Sequence similarities	Belongs to the protein disulfide isomerase family. Contains 3 thioredoxin domains.

Ontologies

Keywords
Cellular component	Endoplasmic reticulum
Domain	Redox-active center Repeat Signal
Molecular function	Isomerase
PTM	Disulfide bond
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cell redox homeostasis Inferred from electronic annotation. Source: InterPro embryonic development ending in birth or egg hatching Inferred from mutant phenotype. Source: WormBase positive regulation of growth rate Inferred from mutant phenotype. Source: WormBase positive regulation of multicellular organism growth Inferred from mutant phenotype. Source: WormBase reproduction Inferred from mutant phenotype. Source: WormBase
Cellular component	endoplasmic reticulum lumen Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	protein binding Inferred from electronic annotation. Source: InterPro protein disulfide isomerase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 21	21	Potential
	Chain	22 – 618	597	Probable protein disulfide-isomerase A4		PRO_0000034232
Regions
	Domain	22 – 139	118	Thioredoxin 1
	Domain	138 – 254	117	Thioredoxin 2
	Domain	480 – 609	130	Thioredoxin 3
	Motif	615 – 618	4	Prevents secretion from ER
Amino acid modifications
	Disulfide bond	65 ↔ 68		Redox-active By similarity
	Disulfide bond	176 ↔ 179		Redox-active By similarity
	Disulfide bond	529 ↔ 532		Redox-active By similarity

Sequences

	Sequence		Length	Mass (Da)
	P34329-1 [UniParc]. Last modified February 21, 2001. Version 2. Checksum: 1DC0207A71444220	FASTA	618	69,797
	10 20 30 40 50 60 MMFDRRFFAL VVLLCVSAVR STEDASDDEL NYEMDEGVVV LTDKNFDAFL KKNPSVLVKF 70 80 90 100 110 120 YAPWCGHCKH LAPEYEKASS KVSIPLAKVD ATVETELGKR FEIQGYPTLK FWKDGKGPND 130 140 150 160 170 180 YDGGRDEAGI VEWVESRVDP NYKPPPEEVV TLTTENFDDF ISNNELVLVE FYAPWCGHCK 190 200 210 220 230 240 KLAPEYEKAA QKLKAQGSKV KLGKVDATIE KDLGTKYGVS GYPTMKIIRN GRRFDYNGPR 250 260 270 280 290 300 EAAGIIKYMT DQSKPAAKKL PKLKDVERFM SKDDVTIIGF FATEDSTAFE AFSDSAEMLR 310 320 330 340 350 360 EEFKTMGHTS DPAAFKKWDA KPNDIIIFYP SLFHSKFEPK SRTYNKAAAT SEDLLAFFRE 370 380 390 400 410 420 HSAPLVGKMT KKNAATRYTK KPLVVVYYNA DFSVQYREGS EYWRSKVLNI AQKYQKDKYK 430 440 450 460 470 480 FAVADEEEFA KELEELGLGD SGLEHNVVVF GYDGKKYPMN PDEFDGELDE NLEAFMKQIS 490 500 510 520 530 540 SGKAKAHVKS APAPKDDKGP VKTVVGSNFD KIVNDESKDV LIEFYAPWCG HCKSFESKYV 550 560 570 580 590 600 ELAQALKKTQ PNVVLAKMDA TINDAPSQFA VEGFPTIYFA PAGKKSEPIK YSGNRDLEDL 610 KKFMTKHGVK SFQKKDEL

References

[1]	"2.2 Mb of contiguous nucleotide sequence from chromosome III of C. elegans." Wilson R., Ainscough R., Anderson K., Baynes C., Berks M., Bonfield J., Burton J., Connell M., Copsey T., Cooper J., Coulson A., Craxton M., Dear S., Du Z., Durbin R., Favello A., Fraser A., Fulton L. , Gardner A., Green P., Hawkins T., Hillier L., Jier M., Johnston L., Jones M., Kershaw J., Kirsten J., Laisster N., Latreille P., Lightning J., Lloyd C., Mortimore B., O'Callaghan M., Parsons J., Percy C., Rifken L., Roopra A., Saunders D., Shownkeen R., Sims M., Smaldon N., Smith A., Smith M., Sonnhammer E., Staden R., Sulston J., Thierry-Mieg J., Thomas K., Vaudin M., Vaughan K., Waterston R., Watson A., Weinstock L., Wilkinson-Sproat J., Wohldman P. Nature 368:32-38(1994) [PubMed: 7906398] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Bristol N2.
[2]	"Genome sequence of the nematode C. elegans: a platform for investigating biology." The C. elegans sequencing consortium Science 282:2012-2018(1998) [PubMed: 9851916] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Bristol N2.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	L15188 Genomic DNA. Translation: AAM22024.1.
PIR	S44756.
RefSeq	NP_498775.2.
UniGene	Cel.17287
3D structure databases
HSSP	HSSP built from PDB template 1MEK based on UniProtKB P07237.
ModBase	Search...
Protein-protein interaction databases
IntAct	P34329. 1 interaction.
Genome annotation databases
Ensembl	C14B9.2.1; C14B9.2.1; C14B9.2; Caenorhabditis elegans. [Genome view] C14B9.2.2; C14B9.2.2; C14B9.2; Caenorhabditis elegans. [Genome view]
GeneID	176147.
KEGG	cel:C14B9.2.
UCSC	C14B9.2.1. c. elegans.
Organism-specific databases
CTD	176147.
WormBase	WBGene00015752. C14B9.2.
WormPep	C14B9.2. CE30601. [WorfDB]
Phylogenomic databases
OMA	RAATQFW.
Enzyme and pathway databases
BRENDA	5.3.4.1. 672.
Gene expression databases
ArrayExpress	P34329.
Family and domain databases
InterPro	IPR005788. Disulphide_isomerase. IPR000886. ER_target_seq_motif. IPR005792. Prot_disulphide_isomerase. IPR017068. Protein_diS-isomerase_A4. IPR017936. Thioredoxin-like. IPR006662. Thioredoxin-like_subdom. IPR017937. Thioredoxin_CS. IPR013766. Thioredoxin_domain. IPR012335. Thioredoxin_fold. [Graphical view]
Gene3D	G3DSA:3.40.30.10. Thioredoxin_fold. 3 hits.
Pfam	PF00085. Thioredoxin. 3 hits. [Graphical view]
PIRSF	PIRSF036862. Disulphide_isom_A4. 1 hit.
PRINTS	PR00421. THIOREDOXIN.
TIGRFAMs	TIGR01130. ER_PDI_fam. 1 hit. TIGR01126. pdi_dom. 3 hits.
PROSITE	PS00014. ER_TARGET. 1 hit. PS00194. THIOREDOXIN_1. 3 hits. PS51352. THIOREDOXIN_2. 3 hits. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	891332.

Entry information

Entry name

PDIA4_CAEEL

Accession

Primary (citable) accession number: P34329

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1994
Last sequence update:	February 21, 2001
Last modified:	November 3, 2009
This is version 83 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Caenorhabditis annotation project