P34308 (CAN_CAEEL) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Calpain clp-1
EC=3.4.22.-

Gene names

Name:	clp-1
ORF Names:	C06G4.2/C06G4.3

Organism

Caenorhabditis elegans [Reference proteome]

Taxonomic identifier

6239 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Protostomia › Ecdysozoa › Nematoda › Chromadorea › Rhabditida › Rhabditoidea › Rhabditidae › Peloderinae › Caenorhabditis

Protein attributes

Sequence length	780 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Calcium-regulated protease involved in necrotic cell death. Ref.3
Sequence similarities	Belongs to the peptidase C2 family. Contains 1 calpain catalytic domain.

Ontologies

Keywords
Coding sequence diversity	Alternative splicing
Molecular function	Hydrolase Protease Thiol protease
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	positive regulation of necrotic cell death Inferred from genetic interaction PubMed 22157748. Source: WormBase proteolysis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	M band Inferred from direct assay PubMed 22479198. Source: WormBase
Molecular_function	calcium-dependent cysteine-type endopeptidase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Alternative products

This entry describes 4 isoforms produced by alternative splicing. [Align] [Select]

Note: Experimental confirmation may be lacking for some isoforms.

Isoform a (identifier: P34308-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Note: No experimental confirmation available.

Isoform b (identifier: P34308-2)

The sequence of this isoform differs from the canonical sequence as follows:
     76-121: Missing.
     708-708: D → DMNN

Note: No experimental confirmation available.

Isoform c (identifier: P34308-3)

The sequence of this isoform differs from the canonical sequence as follows:
     77-113: SNYDQGGNGNSGDQQKRKRDMAKDLIGGIFDNVVNRK → IFIFKIVRQKFPKNSSSFFCVRKHLDSLKTSPCGLDQ
     114-780: Missing.

Note: No experimental confirmation available.

Isoform d (identifier: P34308-4)

The sequence of this isoform differs from the canonical sequence as follows:
     77-100: SNYDQGGNGNSGDQQKRKRDMAKD → GGGSGGGGGGNNIGSLVGSLIGGG
     101-121: Missing.

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 780	780	Calpain clp-1		PRO_0000207734
Regions
	Domain	316 – 611	296	Calpain catalytic
	Compositional bias	21 – 288	268	Gly-rich
Sites
	Active site	371	1	By similarity
	Active site	527	1	By similarity
	Active site	551	1	By similarity
Natural variations
	Alternative sequence	76 – 121	46	Missing in isoform b.		VSP_005247
	Alternative sequence	77 – 113	37	SNYDQ…VVNRK → IFIFKIVRQKFPKNSSSFFC VRKHLDSLKTSPCGLDQ in isoform c.		VSP_005249
	Alternative sequence	77 – 100	24	SNYDQ…DMAKD → GGGSGGGGGGNNIGSLVGSL IGGG in isoform d.		VSP_005248
	Alternative sequence	101 – 121	21	Missing in isoform d.		VSP_005250
	Alternative sequence	114 – 780	667	Missing in isoform c.		VSP_005251
	Alternative sequence	708	1	D → DMNN in isoform b.		VSP_005252

Sequences

	Sequence		Length	Mass (Da)
	Isoform a [UniParc]. Last modified July 5, 2005. Version 4. Checksum: C1DFA4E5671F7835	FASTA	780	83,643
	10 20 30 40 50 60 MADDEEEIIQ KVEVKPDEFN GLIGSIAGNL IRDKVGGAGG DILGGLASNF FGGGGGGGGG 70 80 90 100 110 120 GGGGGFGGGN GGFGGGSNYD QGGNGNSGDQ QKRKRDMAKD LIGGIFDNVV NRKGKKEQDN 130 140 150 160 170 180 YGGGGNYGGG GGNQGGGGGG GFNFNDIGGL INSMGGGGGG GQRQGGGGGG FGDILGGIGS 190 200 210 220 230 240 LIGGGGGGQY NGGGGNVNPN NLNGGMVNVI GNLIGEAAHR FLGVDPGTGR IIGAVAGNVI 250 260 270 280 290 300 MGLGGKDNSL GNIGKVILDN IISGKFRRDV DPFVRPGPDP DRGGGGSGPS PISPRPTTEP 310 320 330 340 350 360 QDFYELRDQC LESKRLFEDP QFLANDSSLF FSKRPPKRVE WLRPGEITRE PQLITEGHSR 370 380 390 400 410 420 FDVIQGELGD CWLLAAAANL TLKDELFYRV VPPDQSFTEN YAGIFHFQFW QYGKWVDVVI 430 440 450 460 470 480 DDRLPTSNGE LLYMHSASNN EFWSALLEKA YAKLFGSYEA LKGGTTSEAL EDMTGGLTEF 490 500 510 520 530 540 IDLKNPPRNL MQMMMRGFEM GSLFGCSIEA DPNVWEAKMS NGLVKGHAYS ITGCRIVDGP 550 560 570 580 590 600 NGQTCILRIR NPWGNEQEWN GPWSDNSREW RSVPDSVKQD MGLKFDHDGE FWMSFDDFMR 610 620 630 640 650 660 NFEKMEICNL GPDVMDEVYQ MTGVKAAGMV WAANTHDGAW VRNQTAGGCR NYINTFANNP 670 680 690 700 710 720 QFRVQLTDSD PDDDDELCTV IFAVLQKYRR NLKQDGLDNV PIGFAVYDAG NNRGRLSKQF 730 740 750 760 770 780 FAANKSAMRS AAFINLREMT GRFRVPPGNY VVVPSTFEPN EEAEFMLRVY TNGFIESEEL
	Isoform b [UniParc]. Checksum: 0B91CF87AD6FA281	FASTA	737	78,904
	10 20 30 40 50 60 MADDEEEIIQ KVEVKPDEFN GLIGSIAGNL IRDKVGGAGG DILGGLASNF FGGGGGGGGG 70 80 90 100 110 120 GGGGGFGGGN GGFGGGGGGN YGGGGGNQGG GGGGGFNFND IGGLINSMGG GGGGGQRQGG 130 140 150 160 170 180 GGGGFGDILG GIGSLIGGGG GGQYNGGGGN VNPNNLNGGM VNVIGNLIGE AAHRFLGVDP 190 200 210 220 230 240 GTGRIIGAVA GNVIMGLGGK DNSLGNIGKV ILDNIISGKF RRDVDPFVRP GPDPDRGGGG 250 260 270 280 290 300 SGPSPISPRP TTEPQDFYEL RDQCLESKRL FEDPQFLAND SSLFFSKRPP KRVEWLRPGE 310 320 330 340 350 360 ITREPQLITE GHSRFDVIQG ELGDCWLLAA AANLTLKDEL FYRVVPPDQS FTENYAGIFH 370 380 390 400 410 420 FQFWQYGKWV DVVIDDRLPT SNGELLYMHS ASNNEFWSAL LEKAYAKLFG SYEALKGGTT 430 440 450 460 470 480 SEALEDMTGG LTEFIDLKNP PRNLMQMMMR GFEMGSLFGC SIEADPNVWE AKMSNGLVKG 490 500 510 520 530 540 HAYSITGCRI VDGPNGQTCI LRIRNPWGNE QEWNGPWSDN SREWRSVPDS VKQDMGLKFD 550 560 570 580 590 600 HDGEFWMSFD DFMRNFEKME ICNLGPDVMD EVYQMTGVKA AGMVWAANTH DGAWVRNQTA 610 620 630 640 650 660 GGCRNYINTF ANNPQFRVQL TDSDPDDDDE LCTVIFAVLQ KYRRNLKQDG LDNVPIGFAV 670 680 690 700 710 720 YDMNNAGNNR GRLSKQFFAA NKSAMRSAAF INLREMTGRF RVPPGNYVVV PSTFEPNEEA 730 EFMLRVYTNG FIESEEL
	Isoform c [UniParc]. Checksum: 2DDF5D0C0A40C297	FASTA	113	11,283
	10 20 30 40 50 60 MADDEEEIIQ KVEVKPDEFN GLIGSIAGNL IRDKVGGAGG DILGGLASNF FGGGGGGGGG 70 80 90 100 110 GGGGGFGGGN GGFGGGIFIF KIVRQKFPKN SSSFFCVRKH LDSLKTSPCG LDQ
	Isoform d [UniParc]. Checksum: 5CC728E8C5372689	FASTA	759	80,442
	10 20 30 40 50 60 MADDEEEIIQ KVEVKPDEFN GLIGSIAGNL IRDKVGGAGG DILGGLASNF FGGGGGGGGG 70 80 90 100 110 120 GGGGGFGGGN GGFGGGGGGS GGGGGGNNIG SLVGSLIGGG GGGGNYGGGG GNQGGGGGGG 130 140 150 160 170 180 FNFNDIGGLI NSMGGGGGGG QRQGGGGGGF GDILGGIGSL IGGGGGGQYN GGGGNVNPNN 190 200 210 220 230 240 LNGGMVNVIG NLIGEAAHRF LGVDPGTGRI IGAVAGNVIM GLGGKDNSLG NIGKVILDNI 250 260 270 280 290 300 ISGKFRRDVD PFVRPGPDPD RGGGGSGPSP ISPRPTTEPQ DFYELRDQCL ESKRLFEDPQ 310 320 330 340 350 360 FLANDSSLFF SKRPPKRVEW LRPGEITREP QLITEGHSRF DVIQGELGDC WLLAAAANLT 370 380 390 400 410 420 LKDELFYRVV PPDQSFTENY AGIFHFQFWQ YGKWVDVVID DRLPTSNGEL LYMHSASNNE 430 440 450 460 470 480 FWSALLEKAY AKLFGSYEAL KGGTTSEALE DMTGGLTEFI DLKNPPRNLM QMMMRGFEMG 490 500 510 520 530 540 SLFGCSIEAD PNVWEAKMSN GLVKGHAYSI TGCRIVDGPN GQTCILRIRN PWGNEQEWNG 550 560 570 580 590 600 PWSDNSREWR SVPDSVKQDM GLKFDHDGEF WMSFDDFMRN FEKMEICNLG PDVMDEVYQM 610 620 630 640 650 660 TGVKAAGMVW AANTHDGAWV RNQTAGGCRN YINTFANNPQ FRVQLTDSDP DDDDELCTVI 670 680 690 700 710 720 FAVLQKYRRN LKQDGLDNVP IGFAVYDAGN NRGRLSKQFF AANKSAMRSA AFINLREMTG 730 740 750 RFRVPPGNYV VVPSTFEPNE EAEFMLRVYT NGFIESEEL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"2.2 Mb of contiguous nucleotide sequence from chromosome III of C. elegans." Wilson R., Ainscough R., Anderson K., Baynes C., Berks M., Bonfield J., Burton J., Connell M., Copsey T., Cooper J., Coulson A., Craxton M., Dear S., Du Z., Durbin R., Favello A., Fraser A., Fulton L. , Gardner A., Green P., Hawkins T., Hillier L., Jier M., Johnston L., Jones M., Kershaw J., Kirsten J., Laisster N., Latreille P., Lightning J., Lloyd C., Mortimore B., O'Callaghan M., Parsons J., Percy C., Rifken L., Roopra A., Saunders D., Shownkeen R., Sims M., Smaldon N., Smith A., Smith M., Sonnhammer E., Staden R., Sulston J., Thierry-Mieg J., Thomas K., Vaudin M., Vaughan K., Waterston R., Watson A., Weinstock L., Wilkinson-Sproat J., Wohldman P. Nature 368:32-38(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Bristol N2.
[2]	"Genome sequence of the nematode C. elegans: a platform for investigating biology." The C. elegans sequencing consortium Science 282:2012-2018(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], ALTERNATIVE SPLICING. Strain: Bristol N2.
[3]	"Specific aspartyl and calpain proteases are required for neurodegeneration in C. elegans." Syntichaki P., Xu K., Driscoll M., Tavernarakis N. Nature 419:939-944(2002) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	FO080399 Genomic DNA. Translation: CCD63432.1. FO080399 Genomic DNA. Translation: CCD63433.1. FO080399 Genomic DNA. Translation: CCD63434.1. FO080399 Genomic DNA. Translation: CCD63435.1.
PIR	S44749. S44750.
RefSeq	NP_498740.2. NM_066339.5. NP_498741.3. NM_066340.4. NP_741237.1. NM_171886.4. NP_741238.1. NM_171201.1.
UniGene	Cel.17311.
3D structure databases
ProteinModelPortal	P34308.
SMR	P34308. Positions 181-259, 301-771.
ModBase	Search...
Protein family/group databases
MEROPS	C02.A03.
Proteomic databases
PaxDb	P34308.
PRIDE	P34308.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblMetazoa	C06G4.2a.1; C06G4.2a.1; C06G4.2. C06G4.2a.2; C06G4.2a.2; C06G4.2.
GeneID	176122.
KEGG	cel:CELE_C06G4.2.
UCSC	C06G4.2a.1. c. elegans.
Organism-specific databases
CTD	176122.
WormBase	C06G4.2a; CE37743; WBGene00000542; clp-1. C06G4.2b; CE37744; WBGene00000542; clp-1. C06G4.2c; CE00517; WBGene00000542; clp-1. C06G4.2d; CE30486; WBGene00000542; clp-1.
Phylogenomic databases
eggNOG	NOG327523.
GeneTree	ENSGT00700000104110.
HOGENOM	HOG000020136.
InParanoid	P34308.
KO	K08585.
OMA	EGHSRFD.
Family and domain databases
InterPro	IPR022684. Calpain_cysteine_protease. IPR022682. Calpain_domain_III. IPR022683. Calpain_III. IPR000169. Pept_cys_AS. IPR001300. Peptidase_C2_calpain_cat. [Graphical view]
Pfam	PF01067. Calpain_III. 1 hit. PF00648. Peptidase_C2. 1 hit. [Graphical view]
PRINTS	PR00704. CALPAIN.
SMART	SM00720. calpain_III. 1 hit. SM00230. CysPc. 1 hit. [Graphical view]
SUPFAM	SSF49758. Peptidase_C2. 1 hit.
PROSITE	PS50203. CALPAIN_CAT. 1 hit. PS00640. THIOL_PROTEASE_ASN. False negative. PS00139. THIOL_PROTEASE_CYS. 1 hit. PS00639. THIOL_PROTEASE_HIS. False negative. [Graphical view]
ProtoNet	Search...
Other
NextBio	891208.

Entry information

Entry name

CAN_CAEEL

Accession

Primary (citable) accession number: P34308
Secondary accession number(s): P34309, Q5DX51, Q5DX52

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1994
Last sequence update:	July 5, 2005
Last modified:	May 1, 2013
This is version 105 of the entry and version 4 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Caenorhabditis annotation project