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Protein

Glutamate receptor 1

Gene

glr-1

Organism
Caenorhabditis elegans
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Non-NMDA (N-methyl-D-aspartate) ionotropic glutamate receptor. L-glutamate acts as an excitatory neurotransmitter at many synapses in the central nervous system. The postsynaptic actions of glutamate are mediated by a variety of receptors that are named according to their selective agonists. May contribute to a sensory discrimination between mechanical and chemical stimuli.1 Publication

GO - Molecular functioni

  • AMPA glutamate receptor activity Source: WormBase
  • enzyme binding Source: WormBase
  • extracellular-glutamate-gated ion channel activity Source: InterPro
  • ionotropic glutamate receptor activity Source: WormBase

GO - Biological processi

  • adult locomotory behavior Source: UniProtKB
  • associative learning Source: WormBase
  • feeding behavior Source: WormBase
  • forward locomotion Source: UniProtKB
  • habituation Source: WormBase
  • hyperosmotic response Source: WormBase
  • ionotropic glutamate receptor signaling pathway Source: WormBase
  • regulation of backward locomotion Source: WormBase
  • response to mechanical stimulus Source: WormBase
  • sensory perception of bitter taste Source: WormBase
  • short-term memory Source: WormBase
  • synaptic transmission, glutamatergic Source: WormBase
  • thigmotaxis Source: WormBase
Complete GO annotation...

Keywords - Molecular functioni

Ion channel, Ligand-gated ion channel, Receptor

Keywords - Biological processi

Ion transport, Transport

Enzyme and pathway databases

ReactomeiR-CEL-204005. COPII (Coat Protein 2) Mediated Vesicle Transport.
R-CEL-399710. Activation of AMPA receptors.
R-CEL-438066. Unblocking of NMDA receptor, glutamate binding and activation.
R-CEL-5694530. Cargo concentration in the ER.

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamate receptor 1Imported
Gene namesi
Name:glr-1Imported
ORF Names:C06E1.4Imported
OrganismiCaenorhabditis elegans
Taxonomic identifieri6239 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis
Proteomesi
  • UP000001940 Componenti: Chromosome III

Organism-specific databases

WormBaseiC06E1.4; CE31407; WBGene00001612; glr-1.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini26 – 591566ExtracellularSequence analysisAdd
BLAST
Transmembranei592 – 61221HelicalSequence analysisAdd
BLAST
Topological domaini613 – 66856CytoplasmicSequence analysisAdd
BLAST
Transmembranei669 – 68921HelicalSequence analysisAdd
BLAST
Topological domaini690 – 855166ExtracellularSequence analysisAdd
BLAST
Transmembranei856 – 87621HelicalSequence analysisAdd
BLAST
Topological domaini877 – 96286CytoplasmicSequence analysisAdd
BLAST

GO - Cellular componenti

  • cell junction Source: UniProtKB-KW
  • cell surface Source: UniProtKB
  • dendrite Source: UniProtKB
  • ionotropic glutamate receptor complex Source: WormBase
  • neuronal cell body Source: UniProtKB
  • neuron projection Source: WormBase
  • neuron projection membrane Source: WormBase
  • perinuclear endoplasmic reticulum Source: UniProtKB
  • plasma membrane Source: WormBase
  • postsynaptic density Source: WormBase
  • postsynaptic membrane Source: UniProtKB
  • recycling endosome Source: UniProtKB-SubCell
  • synapse Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cell junction, Cell membrane, Endoplasmic reticulum, Endosome, Membrane, Postsynaptic cell membrane, Synapse

Pathology & Biotechi

Disruption phenotypei

Decreased frequency of reversals, suppression of hyperreversal phenotype of cni-1 mutants, decreased tactile response and increased osmotic avoidance.1 Publication

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi888 – 8881K → R: Increased levels of glr-1. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2525Sequence analysisAdd
BLAST
Chaini26 – 962937Glutamate receptor 1PRO_0000011590Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi190 – 1901N-linked (GlcNAc...)Sequence analysis
Glycosylationi220 – 2201N-linked (GlcNAc...)Sequence analysis
Glycosylationi275 – 2751N-linked (GlcNAc...)Sequence analysis
Glycosylationi333 – 3331N-linked (GlcNAc...)Sequence analysis
Glycosylationi441 – 4411N-linked (GlcNAc...)Sequence analysis
Glycosylationi482 – 4821N-linked (GlcNAc...)Sequence analysis
Glycosylationi852 – 8521N-linked (GlcNAc...)Sequence analysis

Post-translational modificationi

Ubiquitinated. Deubiquitinated by usp-46 which prevents its degradation.2 Publications
Glycosylated.1 Publication

Keywords - PTMi

Glycoprotein, Ubl conjugation

Proteomic databases

EPDiP34299.
PaxDbiP34299.
PRIDEiP34299.

Expressioni

Tissue specificityi

Command interneurons of the locomotory control circuit (AIB, AVA, AVB, AVD, AVE and PVC) and motor neurons (RMD, RIM, SMD, AVG, PVQ and URY).2 Publications

Interactioni

Subunit structurei

Interacts with sol-1 (PubMed:14749834). Interacts with cni-1; the interaction negatively regulates export of glr-1 from the endoplasmic reticulum to synapses (PubMed:24094107). Interacts with usp-46; the interaction results in deubiquitination of glr-1 (PubMed:24356955).3 Publications

GO - Molecular functioni

  • enzyme binding Source: WormBase

Protein-protein interaction databases

BioGridi41408. 3 interactions.
STRINGi6239.C06E1.4.

Structurei

3D structure databases

ProteinModelPortaliP34299.
SMRiP34299. Positions 78-881.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG1054. Eukaryota.
ENOG410XPSH. LUCA.
GeneTreeiENSGT00760000118920.
HOGENOMiHOG000234372.
InParanoidiP34299.
KOiK05313.
OMAiLINIRSA.
OrthoDBiEOG7C2R0J.
PhylomeDBiP34299.

Family and domain databases

InterProiIPR001828. ANF_lig-bd_rcpt.
IPR019594. Glu/Gly-bd.
IPR001508. Iono_rcpt_met.
IPR001320. Iontro_rcpt.
IPR028082. Peripla_BP_I.
IPR001638. Solute-binding_3/MltF_N.
[Graphical view]
PfamiPF01094. ANF_receptor. 1 hit.
PF00060. Lig_chan. 1 hit.
PF10613. Lig_chan-Glu_bd. 1 hit.
[Graphical view]
PRINTSiPR00177. NMDARECEPTOR.
SMARTiSM00918. Lig_chan-Glu_bd. 1 hit.
SM00062. PBPb. 1 hit.
SM00079. PBPe. 1 hit.
[Graphical view]
SUPFAMiSSF53822. SSF53822. 2 hits.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P34299-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MFSSFSFLNM FGVLFTVFNL TVVQPYPSHI IIKSFGNNEE VSRVALKAME
60 70 80 90 100
YTSDHINSRD DVPFKLAFDH RVVEEGAAVS WNMVNAVCDE LKEGAMALLS
110 120 130 140 150
SVDGKGREGI RGVSDALEMP LVSLTALSND DHQQQQFGNL FEVSVRPPIS
160 170 180 190 200
ELLADFIVHK GWGEVLVLID PVHASLHLPS LWRHLRTRTN TSVKASMFDL
210 220 230 240 250
PADEKQFEAY LMQFNMMRNN ETNRILIDCA SPKRLKKLLI NIRSAQFNQA
260 270 280 290 300
NYHYVLANYD FLPYDQEMFQ NGNINISGFN IINKDGREYW SLKKHLKTSS
310 320 330 340 350
SLGGGDDVSV EAAVGHDAML VTWHGFAKCL QANDSLFHGT FRHRRFFNRG
360 370 380 390 400
FPGIYCDPLS DRSHPNRPFS SFEHGKTIGV AFRNMKIGHK EGTLTGNIEF
410 420 430 440 450
DRFGNRKNFD VSIVDLVSNT KATFNSKEVL AWRQGVGFFS NRTVAQHSRK
460 470 480 490 500
SQNDHKDNQV IVLTNLVAPF VMIKRECLEM ANLTECQGNN KFEGFCIDLL
510 520 530 540 550
KLLADKIEEF NYEIKLGTKA GSKQADGSWD GMIGELLSGR AHAVVASLTI
560 570 580 590 600
NQERERVVDF SKPFMTTGIS IMIKKPDKQE FSVFSFMQPL STEIWMYIIF
610 620 630 640 650
AYIGVSVVIF LVSRFSPYEW RVEETSRGGF TISNDFSVYN CLWFTLAAFM
660 670 680 690 700
QQGTDILPRS ISGRIASSAW WFFTMIIVSS YTANLAAFLT LEKMQAPIES
710 720 730 740 750
VEDLAKQSKI KYGIQGGGST ASFFKYSSVQ IYQRMWRYME SQVPPVFVAS
760 770 780 790 800
YAEGIERVRS HKGRYAFLLE ATANEYENTR KPCDTMKVGA NLNSIGYGIA
810 820 830 840 850
TPFGSDWKDH INLAILALQE RGELKKLENK WWYDRGQCDA GITVDGSSAS
860 870 880 890 900
LNLSKVAGIF YILMGGMVIS MLAALGEFLY RSRIEARKSN SNSMVANFAK
910 920 930 940 950
NLKSALSSQL RLSVEGGAVA QPGSQSHNAI RRQQVAAFLP ANEKEAFNNV
960
DRPANTLYNT AV
Length:962
Mass (Da):108,142
Last modified:March 28, 2003 - v2
Checksum:i25488406566A5583
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U34661 mRNA. Translation: AAA92006.1.
FO080280 Genomic DNA. Translation: CCD62564.1.
PIRiB88533.
S60225.
RefSeqiNP_498887.2. NM_066486.6.
UniGeneiCel.6005.

Genome annotation databases

EnsemblMetazoaiC06E1.4; C06E1.4; WBGene00001612.
GeneIDi176204.
KEGGicel:CELE_C06E1.4.
UCSCiC06E1.4. c. elegans.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U34661 mRNA. Translation: AAA92006.1.
FO080280 Genomic DNA. Translation: CCD62564.1.
PIRiB88533.
S60225.
RefSeqiNP_498887.2. NM_066486.6.
UniGeneiCel.6005.

3D structure databases

ProteinModelPortaliP34299.
SMRiP34299. Positions 78-881.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi41408. 3 interactions.
STRINGi6239.C06E1.4.

Proteomic databases

EPDiP34299.
PaxDbiP34299.
PRIDEiP34299.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiC06E1.4; C06E1.4; WBGene00001612.
GeneIDi176204.
KEGGicel:CELE_C06E1.4.
UCSCiC06E1.4. c. elegans.

Organism-specific databases

CTDi176204.
WormBaseiC06E1.4; CE31407; WBGene00001612; glr-1.

Phylogenomic databases

eggNOGiKOG1054. Eukaryota.
ENOG410XPSH. LUCA.
GeneTreeiENSGT00760000118920.
HOGENOMiHOG000234372.
InParanoidiP34299.
KOiK05313.
OMAiLINIRSA.
OrthoDBiEOG7C2R0J.
PhylomeDBiP34299.

Enzyme and pathway databases

ReactomeiR-CEL-204005. COPII (Coat Protein 2) Mediated Vesicle Transport.
R-CEL-399710. Activation of AMPA receptors.
R-CEL-438066. Unblocking of NMDA receptor, glutamate binding and activation.
R-CEL-5694530. Cargo concentration in the ER.

Miscellaneous databases

PROiP34299.

Family and domain databases

InterProiIPR001828. ANF_lig-bd_rcpt.
IPR019594. Glu/Gly-bd.
IPR001508. Iono_rcpt_met.
IPR001320. Iontro_rcpt.
IPR028082. Peripla_BP_I.
IPR001638. Solute-binding_3/MltF_N.
[Graphical view]
PfamiPF01094. ANF_receptor. 1 hit.
PF00060. Lig_chan. 1 hit.
PF10613. Lig_chan-Glu_bd. 1 hit.
[Graphical view]
PRINTSiPR00177. NMDARECEPTOR.
SMARTiSM00918. Lig_chan-Glu_bd. 1 hit.
SM00062. PBPb. 1 hit.
SM00079. PBPe. 1 hit.
[Graphical view]
SUPFAMiSSF53822. SSF53822. 2 hits.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Mechanosensory signalling in C. elegans mediated by the GLR-1 glutamate receptor."
    Maricq A.V., Peckol E., Driscoll M., Bargmann C.I.
    Nature 378:78-81(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, TISSUE SPECIFICITY.
    Strain: Bristol N2.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Bristol N2.
  3. "Genome sequence of the nematode C. elegans: a platform for investigating biology."
    The C. elegans sequencing consortium
    Science 282:2012-2018(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Bristol N2.
  4. "Differential expression of glutamate receptor subunits in the nervous system of Caenorhabditis elegans and their regulation by the homeodomain protein UNC-42."
    Brockie P.J., Madsen D.M., Zheng Y., Mellem J., Maricq A.V.
    J. Neurosci. 21:1510-1522(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: TISSUE SPECIFICITY.
  5. "Ubiquitin and AP180 regulate the abundance of GLR-1 glutamate receptors at postsynaptic elements in C. elegans."
    Burbea M., Dreier L., Dittman J.S., Grunwald M.E., Kaplan J.M.
    Neuron 35:107-120(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION, UBIQUITINATION, MUTAGENESIS OF LYS-888.
  6. "SOL-1 is a CUB-domain protein required for GLR-1 glutamate receptor function in C. elegans."
    Zheng Y., Mellem J.E., Brockie P.J., Madsen D.M., Maricq A.V.
    Nature 427:451-457(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH SOL-1.
  7. "CDK-5 regulates the abundance of GLR-1 glutamate receptors in the ventral cord of Caenorhabditis elegans."
    Juo P., Harbaugh T., Garriga G., Kaplan J.M.
    Mol. Biol. Cell 18:3883-3893(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION.
  8. "Hypoxia regulates glutamate receptor trafficking through an HIF-independent mechanism."
    Park E.C., Ghose P., Shao Z., Ye Q., Kang L., Xu X.Z., Powell-Coffman J.A., Rongo C.
    EMBO J. 31:1379-1393(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION.
  9. Cited for: INTERACTION WITH CNI-1, SUBCELLULAR LOCATION, GLYCOSYLATION, DISRUPTION PHENOTYPE.
  10. "The WD40-repeat proteins WDR-20 and WDR-48 bind and activate the deubiquitinating enzyme USP-46 to promote the abundance of the glutamate receptor GLR-1 in the ventral nerve cord of Caenorhabditis elegans."
    Dahlberg C.L., Juo P.
    J. Biol. Chem. 289:3444-3456(2014) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH USP-46, SUBCELLULAR LOCATION, DEUBIQUITINATION BY USP46.

Entry informationi

Entry nameiGLR1_CAEEL
AccessioniPrimary (citable) accession number: P34299
Secondary accession number(s): Q17363
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: March 28, 2003
Last modified: June 8, 2016
This is version 138 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programCaenorhabditis annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Caenorhabditis elegans
    Caenorhabditis elegans: entries, gene names and cross-references to WormBase
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.