P34277 (GSTO2_CAEEL) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Probable glutathione transferase omega-2
EC=2.5.1.18

Alternative name(s):
Glutathione-dependent dehydroascorbate reductase
EC=1.8.5.1
Monomethylarsonic acid reductase
Short name=MMA(V) reductase
EC=1.20.4.2

Gene names

Name:	gsto-2
ORF Names:	C02D5.3

Organism

Caenorhabditis elegans [Reference proteome]

Taxonomic identifier

6239 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Protostomia › Ecdysozoa › Nematoda › Chromadorea › Rhabditida › Rhabditoidea › Rhabditidae › Peloderinae › Caenorhabditis

Protein attributes

Sequence length	254 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Exhibits glutathione-dependent thiol transferase activity. Has dehydroascorbate reductase activity and may contribute to the recycling of ascorbic acid. Participates in the biotransformation of inorganic arsenic and reduces monomethylarsonic acid (MMA) By similarity.
Catalytic activity	RX + glutathione = HX + R-S-glutathione. 2 glutathione + dehydroascorbate = glutathione disulfide + ascorbate. Methylarsonate + 2 glutathione = methylarsonite + glutathione disulfide + H2O.
Sequence similarities	Belongs to the GST superfamily. Omega family. Contains 1 GST C-terminal domain. Contains 1 GST N-terminal domain.

Ontologies

Keywords
Domain	Repeat
Molecular function	Oxidoreductase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Cellular_component	cytoplasm Inferred from electronic annotation. Source: InterPro
Molecular_function	glutathione dehydrogenase (ascorbate) activity Inferred from electronic annotation. Source: EC glutathione transferase activity Inferred from electronic annotation. Source: EC methylarsonate reductase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 254	254	Probable glutathione transferase omega-2		PRO_0000185890
Regions
	Domain	25 – 105	81	GST N-terminal
	Domain	110 – 239	130	GST C-terminal
	Region	89 – 90	2	Glutathione binding By similarity
Sites
	Active site	35	1	Nucleophile By similarity
	Binding site	62	1	Glutathione By similarity
	Binding site	75	1	Glutathione; via amide nitrogen and carbonyl oxygen By similarity

Sequences

	Sequence		Length	Mass (Da)
	P34277 [UniParc]. Last modified February 8, 2011. Version 5. Checksum: C648FE7E6889CCB5	FASTA	254	28,799
	10 20 30 40 50 60 MSVLSGLNTK VVKNGDPAPA PPASGTIRIY NMRYCPWAQR ALIFASLKKI PTEVINIHLD 70 80 90 100 110 120 QKPDWFFTKH YKGQVPALEH DEGKKIVIES AVIPEYLDDI YPEPRIIPTD HYEKVQQKLL 130 140 150 160 170 180 LDRISGQLSS AFYGVVQAAK ISDLLKEKLV ELAKAYDTAE ELLTGDFYSG TSKPGFVDYL 190 200 210 220 230 240 IYPNIQRAFW TSHIIKDFPL KVESFPGPNY PKLSKWYKRL DSIPEVIATS QPTETAVEFF 250 KSWIIGAPNF DYGL

References

[1]	"2.2 Mb of contiguous nucleotide sequence from chromosome III of C. elegans." Wilson R., Ainscough R., Anderson K., Baynes C., Berks M., Bonfield J., Burton J., Connell M., Copsey T., Cooper J., Coulson A., Craxton M., Dear S., Du Z., Durbin R., Favello A., Fraser A., Fulton L. , Gardner A., Green P., Hawkins T., Hillier L., Jier M., Johnston L., Jones M., Kershaw J., Kirsten J., Laisster N., Latreille P., Lightning J., Lloyd C., Mortimore B., O'Callaghan M., Parsons J., Percy C., Rifken L., Roopra A., Saunders D., Shownkeen R., Sims M., Smaldon N., Smith A., Smith M., Sonnhammer E., Staden R., Sulston J., Thierry-Mieg J., Thomas K., Vaudin M., Vaughan K., Waterston R., Watson A., Weinstock L., Wilkinson-Sproat J., Wohldman P. Nature 368:32-38(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Bristol N2.
[2]	"Genome sequence of the nematode C. elegans: a platform for investigating biology." The C. elegans sequencing consortium Science 282:2012-2018(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Bristol N2.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	FO080279 Genomic DNA. Translation: CCD62560.1.
PIR	S44745.
RefSeq	NP_871705.3. NM_181976.3.
3D structure databases
ProteinModelPortal	P34277.
SMR	P34277. Positions 8-254.
ModBase	Search...
Proteomic databases
PaxDb	P34277.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblMetazoa	C02D5.3; C02D5.3; C02D5.3.
GeneID	353420.
KEGG	cel:CELE_C02D5.3.
Organism-specific databases
CTD	353420.
WormBase	C02D5.3; CE39555; WBGene00015337; gsto-2.
Phylogenomic databases
eggNOG	NOG288793.
GeneTree	ENSGT00390000005479.
InParanoid	P34277.
OMA	FGQFINA.
Family and domain databases
Gene3D	1.20.1050.10. 1 hit. 3.40.30.10. 1 hit.
InterPro	IPR010987. Glutathione-S-Trfase_C-like. IPR004045. Glutathione_S-Trfase_N. IPR017933. Glutathione_S_Trfase/Cl_chnl_C. IPR004046. GST_C. IPR005442. GST_omega. IPR012336. Thioredoxin-like_fold. [Graphical view]
Pfam	PF00043. GST_C. 1 hit. [Graphical view]
PRINTS	PR01625. GSTRNSFRASEO.
SUPFAM	SSF47616. GST_C_like. 1 hit. SSF52833. Thiordxn-like_fd. 1 hit.
PROSITE	PS50405. GST_CTER. 1 hit. PS50404. GST_NTER. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	953629.

Entry information

Entry name

GSTO2_CAEEL

Accession

Primary (citable) accession number: P34277
Secondary accession number(s): Q2L6Y9 , Q304F1, Q304F2, Q8I7N0

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1994
Last sequence update:	February 8, 2011
Last modified:	May 1, 2013
This is version 95 of the entry and version 5 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Caenorhabditis annotation project