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P34253

- KTI12_YEAST

UniProt

P34253 - KTI12_YEAST

Protein

Protein KTI12

Gene

KTI12

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 109 (01 Oct 2014)
      Sequence version 2 (01 Jun 1994)
      Previous versions | rss
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    Functioni

    Elongator-associated factor that is not a structural subunit but rather transiently contacts Elongator, presumably to promote its interaction with elongation competent pol II. Regulates Elongator complex gamma-toxin target (TOT) activity, resulting in G1 block by Kluyveromyces lactis toxin zymocin (pGKL1 killer toxin), probably through regulation of the Elongator IKI3 subunit phosphorylation state. Required for an early step in synthesis of 5-methoxycarbonylmethyl (mcm5) and 5-carbamoylmethyl (ncm5) groups present on uridines at the wobble position in tRNA.7 Publications

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi8 – 158ATPSequence Analysis

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. chromatin binding Source: SGD

    GO - Biological processi

    1. cell growth Source: SGD
    2. regulation of transcription from RNA polymerase II promoter Source: SGD
    3. transcription, DNA-templated Source: UniProtKB-KW
    4. tRNA wobble uridine modification Source: SGD

    Keywords - Biological processi

    Transcription, Transcription regulation

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciYEAST:G3O-31896-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Protein KTI12
    Alternative name(s):
    Gamma-toxin target protein 4
    Killer toxin insensitivity protein 12
    Gene namesi
    Name:KTI12
    Ordered Locus Names:YKL110C
    ORF Names:YKL446, YKL500
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome XI

    Organism-specific databases

    CYGDiYKL110c.
    SGDiS000001593. KTI12.

    Subcellular locationi

    Cytoplasm 1 Publication. Nucleus 1 Publication

    GO - Cellular componenti

    1. cytoplasm Source: SGD
    2. nucleus Source: SGD

    Keywords - Cellular componenti

    Cytoplasm, Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 313313Protein KTI12PRO_0000084335Add
    BLAST

    Proteomic databases

    MaxQBiP34253.
    PaxDbiP34253.
    PeptideAtlasiP34253.

    Expressioni

    Gene expression databases

    GenevestigatoriP34253.

    Interactioni

    Subunit structurei

    Interacts with the RNA polymerase II elongator complex and RNA polymerase II.6 Publications

    Protein-protein interaction databases

    BioGridi34023. 105 interactions.
    DIPiDIP-1856N.
    IntActiP34253. 2 interactions.
    MINTiMINT-408483.
    STRINGi4932.YKL110C.

    Structurei

    3D structure databases

    ProteinModelPortaliP34253.
    SMRiP34253. Positions 3-183.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the KTI12 family.Curated

    Phylogenomic databases

    eggNOGiCOG4088.
    GeneTreeiENSGT00390000002443.
    HOGENOMiHOG000247843.
    KOiK15456.
    OMAiPCSGKTR.
    OrthoDBiEOG7RBZKK.

    Family and domain databases

    Gene3Di3.40.50.300. 1 hit.
    InterProiIPR013641. Chromatin_KTI12.
    IPR027417. P-loop_NTPase.
    [Graphical view]
    PfamiPF08433. KTI12. 1 hit.
    [Graphical view]
    SUPFAMiSSF52540. SSF52540. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    P34253-1 [UniParc]FASTAAdd to Basket

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    MPLVLFTGYP CSGKTTLAKH LVQLLQSKID ATPSLSKYSI TYHSDESLGI    50
    KHSDYITSQD ERKLRSEIIS AVKRDLSKNK IVIVDSLNYI KGFRYQLHCE 100
    VKNLSTTFCV IQTLCPPETI FEWNKTSNPN PWEPELLNQL IQRYEEPNSS 150
    NRWDSPLFAI LTPQDNITDY IDDICKVVFQ TSKSAKNSGH NDPLSKGLQK 200
    PNSATVLKPA SQSNFIQVLD IETSKIIKTI MNHIKSLTSI GGVSNGTRVI 250
    VSEGITDIND DGCFFVDLPI GNVVTLAQLQ RLKRQFINFN KLRDIDQDRI 300
    GPLFADYLNK NLN 313
    Length:313
    Mass (Da):35,322
    Last modified:June 1, 1994 - v2
    Checksum:i15A9534087A24AE6
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti103 – 1031N → D in AAA66312. (PubMed:1561835)Curated
    Sequence conflicti150 – 1501S → N in AAA66312. (PubMed:1561835)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X77511 Genomic DNA. Translation: CAA54646.1.
    X71133 Genomic DNA. Translation: CAA50447.1.
    S93804 Genomic DNA. Translation: AAB22001.1.
    Z28110 Genomic DNA. Translation: CAA81950.1.
    M29067 Genomic DNA. Translation: AAA66312.1.
    BK006944 Genomic DNA. Translation: DAA09047.1.
    PIRiS37937.
    RefSeqiNP_012812.1. NM_001179676.1.

    Genome annotation databases

    EnsemblFungiiYKL110C; YKL110C; YKL110C.
    GeneIDi853750.
    KEGGisce:YKL110C.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X77511 Genomic DNA. Translation: CAA54646.1 .
    X71133 Genomic DNA. Translation: CAA50447.1 .
    S93804 Genomic DNA. Translation: AAB22001.1 .
    Z28110 Genomic DNA. Translation: CAA81950.1 .
    M29067 Genomic DNA. Translation: AAA66312.1 .
    BK006944 Genomic DNA. Translation: DAA09047.1 .
    PIRi S37937.
    RefSeqi NP_012812.1. NM_001179676.1.

    3D structure databases

    ProteinModelPortali P34253.
    SMRi P34253. Positions 3-183.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 34023. 105 interactions.
    DIPi DIP-1856N.
    IntActi P34253. 2 interactions.
    MINTi MINT-408483.
    STRINGi 4932.YKL110C.

    Proteomic databases

    MaxQBi P34253.
    PaxDbi P34253.
    PeptideAtlasi P34253.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YKL110C ; YKL110C ; YKL110C .
    GeneIDi 853750.
    KEGGi sce:YKL110C.

    Organism-specific databases

    CYGDi YKL110c.
    SGDi S000001593. KTI12.

    Phylogenomic databases

    eggNOGi COG4088.
    GeneTreei ENSGT00390000002443.
    HOGENOMi HOG000247843.
    KOi K15456.
    OMAi PCSGKTR.
    OrthoDBi EOG7RBZKK.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-31896-MONOMER.

    Miscellaneous databases

    NextBioi 974813.
    PROi P34253.

    Gene expression databases

    Genevestigatori P34253.

    Family and domain databases

    Gene3Di 3.40.50.300. 1 hit.
    InterProi IPR013641. Chromatin_KTI12.
    IPR027417. P-loop_NTPase.
    [Graphical view ]
    Pfami PF08433. KTI12. 1 hit.
    [Graphical view ]
    SUPFAMi SSF52540. SSF52540. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Two Saccharomyces cerevisiae genes which control sensitivity to G1 arrest induced by Kluyveromyces lactis toxin."
      Butler A.R., White J.H., Folawiyo Y., Edlin A., Gardiner D., Stark M.J.R.
      Mol. Cell. Biol. 14:6306-6316(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION.
    2. "Complete DNA sequence of yeast chromosome XI."
      Dujon B., Alexandraki D., Andre B., Ansorge W., Baladron V., Ballesta J.P.G., Banrevi A., Bolle P.-A., Bolotin-Fukuhara M., Bossier P., Bou G., Boyer J., Buitrago M.J., Cheret G., Colleaux L., Daignan-Fornier B., del Rey F., Dion C.
      , Domdey H., Duesterhoeft A., Duesterhus S., Entian K.-D., Erfle H., Esteban P.F., Feldmann H., Fernandes L., Fobo G.M., Fritz C., Fukuhara H., Gabel C., Gaillon L., Garcia-Cantalejo J.M., Garcia-Ramirez J.J., Gent M.E., Ghazvini M., Goffeau A., Gonzalez A., Grothues D., Guerreiro P., Hegemann J.H., Hewitt N., Hilger F., Hollenberg C.P., Horaitis O., Indge K.J., Jacquier A., James C.M., Jauniaux J.-C., Jimenez A., Keuchel H., Kirchrath L., Kleine K., Koetter P., Legrain P., Liebl S., Louis E.J., Maia e Silva A., Marck C., Monnier A.-L., Moestl D., Mueller S., Obermaier B., Oliver S.G., Pallier C., Pascolo S., Pfeiffer F., Philippsen P., Planta R.J., Pohl F.M., Pohl T.M., Poehlmann R., Portetelle D., Purnelle B., Puzos V., Ramezani Rad M., Rasmussen S.W., Remacha M.A., Revuelta J.L., Richard G.-F., Rieger M., Rodrigues-Pousada C., Rose M., Rupp T., Santos M.A., Schwager C., Sensen C., Skala J., Soares H., Sor F., Stegemann J., Tettelin H., Thierry A., Tzermia M., Urrestarazu L.A., van Dyck L., van Vliet-Reedijk J.C., Valens M., Vandenbol M., Vilela C., Vissers S., von Wettstein D., Voss H., Wiemann S., Xu G., Zimmermann J., Haasemann M., Becker I., Mewes H.-W.
      Nature 369:371-378(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    3. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    4. "The DNA sequence analysis of the HAP4-LAP4 region on chromosome XI of Saccharomyces cerevisiae suggests the presence of a second aspartate aminotransferase gene in yeast."
      Cheret G., Pallier C., Valens M., Daignan-Fornier B., Fukuhara H., Bolotin-Fukuhara M., Sor F.
      Yeast 9:1259-1265(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-75.
    5. "Sequence of a 10.7 kb segment of yeast chromosome XI identifies the APN1 and the BAF1 loci and reveals one tRNA gene and several new open reading frames including homologs to RAD2 and kinases."
      Jacquier A., Legrain P., Dujon B.
      Yeast 8:121-132(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 75-313.
    6. "Similarity between the transcriptional silencer binding proteins ABF1 and RAP1."
      Diffley J.F.X., Stillman B.
      Science 246:1034-1038(1989) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 103-313.
    7. "Saccharomyces cerevisiae Elongator mutations confer resistance to the Kluyveromyces lactis zymocin."
      Frohloff F., Fichtner L., Jablonowski D., Breunig K.D., Schaffrath R.
      EMBO J. 20:1993-2003(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, INTERACTION WITH THE ELONGATOR COMPLEX.
    8. "Molecular analysis of KTI12/TOT4, a Saccharomyces cerevisiae gene required for Kluyveromyces lactis zymocin action."
      Fichtner L., Frohloff F., Buerkner K., Larsen M., Breunig K.D., Schaffrath R.
      Mol. Microbiol. 43:783-791(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, INTERACTION WITH THE ELONGATOR COMPLEX.
    9. "Protein interactions within Saccharomyces cerevisiae Elongator, a complex essential for Kluyveromyces lactis zymocicity."
      Fichtner L., Frohloff F., Jablonowski D., Stark M.J.R., Schaffrath R.
      Mol. Microbiol. 45:817-826(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH THE ELONGATOR COMPLEX.
    10. "Subunit communications crucial for the functional integrity of the yeast RNA polymerase II elongator (gamma-toxin target (TOT)) complex."
      Frohloff F., Jablonowski D., Fichtner L., Schaffrath R.
      J. Biol. Chem. 278:956-961(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, INTERACTION WITH THE ELONGATOR COMPLEX.
    11. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
    12. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    13. "The yeast elongator histone acetylase requires Sit4-dependent dephosphorylation for toxin-target capacity."
      Jablonowski D., Fichtner L., Stark M.J.R., Schaffrath R.
      Mol. Biol. Cell 15:1459-1469(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, INTERACTION WITH THE ELONGATOR COMPLEX.
    14. "Physical and functional interaction between Elongator and the chromatin-associated Kti12 protein."
      Petrakis T.G., Soegaard T.M.M., Erdjument-Bromage H., Tempst P., Svejstrup J.Q.
      J. Biol. Chem. 280:19454-19460(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, INTERACTION WITH THE ELONGATOR COMPLEX, CHROMATIN BINDING.
    15. "An early step in wobble uridine tRNA modification requires the Elongator complex."
      Huang B., Johansson M.J.O., Bystroem A.S.
      RNA 11:424-436(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.

    Entry informationi

    Entry nameiKTI12_YEAST
    AccessioniPrimary (citable) accession number: P34253
    Secondary accession number(s): D6VXH7, Q05155
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1994
    Last sequence update: June 1, 1994
    Last modified: October 1, 2014
    This is version 109 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 5260 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    3. Yeast chromosome XI
      Yeast (Saccharomyces cerevisiae) chromosome XI: entries and gene names

    External Data

    Dasty 3