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Protein

Protein KTI12

Gene

KTI12

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Elongator-associated factor that is not a structural subunit but rather transiently contacts Elongator, presumably to promote its interaction with elongation competent pol II. Regulates Elongator complex gamma-toxin target (TOT) activity, resulting in G1 block by Kluyveromyces lactis toxin zymocin (pGKL1 killer toxin), probably through regulation of the Elongator IKI3 subunit phosphorylation state. Required for an early step in synthesis of 5-methoxycarbonylmethyl (mcm5) and 5-carbamoylmethyl (ncm5) groups present on uridines at the wobble position in tRNA.7 Publications

Miscellaneous

Present with 5260 molecules/cell in log phase SD medium.1 Publication

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi8 – 15ATPSequence analysis8

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • chromatin binding Source: SGD

GO - Biological processi

  • cell growth Source: SGD
  • regulation of transcription from RNA polymerase II promoter Source: SGD
  • transcription, DNA-templated Source: UniProtKB-KW
  • tRNA wobble uridine modification Source: SGD

Keywordsi

Biological processTranscription, Transcription regulation
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-31896-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Protein KTI12
Alternative name(s):
Gamma-toxin target protein 4
Killer toxin insensitivity protein 12
Gene namesi
Name:KTI12
Ordered Locus Names:YKL110C
ORF Names:YKL446, YKL500
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XI

Organism-specific databases

EuPathDBiFungiDB:YKL110C.
SGDiS000001593. KTI12.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000843351 – 313Protein KTI12Add BLAST313

Proteomic databases

MaxQBiP34253.
PRIDEiP34253.

PTM databases

iPTMnetiP34253.

Interactioni

Subunit structurei

Interacts with the RNA polymerase II elongator complex and RNA polymerase II.6 Publications

Protein-protein interaction databases

BioGridi34023. 299 interactors.
DIPiDIP-1856N.
IntActiP34253. 6 interactors.
MINTiMINT-408483.
STRINGi4932.YKL110C.

Structurei

3D structure databases

ProteinModelPortaliP34253.
SMRiP34253.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the KTI12 family.Curated

Phylogenomic databases

GeneTreeiENSGT00390000002443.
HOGENOMiHOG000247843.
InParanoidiP34253.
KOiK15456.
OMAiEPQSNNR.
OrthoDBiEOG092C3NVY.

Family and domain databases

InterProiView protein in InterPro
IPR013641. KTI12/PSTK.
IPR027417. P-loop_NTPase.
PfamiView protein in Pfam
PF08433. KTI12. 1 hit.
SUPFAMiSSF52540. SSF52540. 1 hit.

Sequencei

Sequence statusi: Complete.

P34253-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPLVLFTGYP CSGKTTLAKH LVQLLQSKID ATPSLSKYSI TYHSDESLGI
60 70 80 90 100
KHSDYITSQD ERKLRSEIIS AVKRDLSKNK IVIVDSLNYI KGFRYQLHCE
110 120 130 140 150
VKNLSTTFCV IQTLCPPETI FEWNKTSNPN PWEPELLNQL IQRYEEPNSS
160 170 180 190 200
NRWDSPLFAI LTPQDNITDY IDDICKVVFQ TSKSAKNSGH NDPLSKGLQK
210 220 230 240 250
PNSATVLKPA SQSNFIQVLD IETSKIIKTI MNHIKSLTSI GGVSNGTRVI
260 270 280 290 300
VSEGITDIND DGCFFVDLPI GNVVTLAQLQ RLKRQFINFN KLRDIDQDRI
310
GPLFADYLNK NLN
Length:313
Mass (Da):35,322
Last modified:June 1, 1994 - v2
Checksum:i15A9534087A24AE6
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti103N → D in AAA66312 (PubMed:1561835).Curated1
Sequence conflicti150S → N in AAA66312 (PubMed:1561835).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X77511 Genomic DNA. Translation: CAA54646.1.
X71133 Genomic DNA. Translation: CAA50447.1.
S93804 Genomic DNA. Translation: AAB22001.1.
Z28110 Genomic DNA. Translation: CAA81950.1.
M29067 Genomic DNA. Translation: AAA66312.1.
BK006944 Genomic DNA. Translation: DAA09047.1.
PIRiS37937.
RefSeqiNP_012812.1. NM_001179676.1.

Genome annotation databases

EnsemblFungiiYKL110C; YKL110C; YKL110C.
GeneIDi853750.
KEGGisce:YKL110C.

Similar proteinsi

Entry informationi

Entry nameiKTI12_YEAST
AccessioniPrimary (citable) accession number: P34253
Secondary accession number(s): D6VXH7, Q05155
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: June 1, 1994
Last modified: September 27, 2017
This is version 130 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XI
    Yeast (Saccharomyces cerevisiae) chromosome XI: entries and gene names