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P34253 (KTI12_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 108. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Protein KTI12
Alternative name(s):
Gamma-toxin target protein 4
Killer toxin insensitivity protein 12
Gene names
Name:KTI12
Ordered Locus Names:YKL110C
ORF Names:YKL446, YKL500
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length313 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Elongator-associated factor that is not a structural subunit but rather transiently contacts Elongator, presumably to promote its interaction with elongation competent pol II. Regulates Elongator complex gamma-toxin target (TOT) activity, resulting in G1 block by Kluyveromyces lactis toxin zymocin (pGKL1 killer toxin), probably through regulation of the Elongator IKI3 subunit phosphorylation state. Required for an early step in synthesis of 5-methoxycarbonylmethyl (mcm5) and 5-carbamoylmethyl (ncm5) groups present on uridines at the wobble position in tRNA. Ref.1 Ref.7 Ref.8 Ref.10 Ref.13 Ref.14 Ref.15

Subunit structure

Interacts with the RNA polymerase II elongator complex and RNA polymerase II. Ref.7 Ref.8 Ref.9 Ref.10 Ref.13 Ref.14

Subcellular location

Cytoplasm. Nucleus Ref.11.

Miscellaneous

Present with 5260 molecules/cell in log phase SD medium.

Sequence similarities

Belongs to the KTI12 family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 313313Protein KTI12
PRO_0000084335

Regions

Nucleotide binding8 – 158ATP Potential

Experimental info

Sequence conflict1031N → D in AAA66312. Ref.5
Sequence conflict1501S → N in AAA66312. Ref.5

Sequences

Sequence LengthMass (Da)Tools
P34253 [UniParc].

Last modified June 1, 1994. Version 2.
Checksum: 15A9534087A24AE6

FASTA31335,322
        10         20         30         40         50         60 
MPLVLFTGYP CSGKTTLAKH LVQLLQSKID ATPSLSKYSI TYHSDESLGI KHSDYITSQD 

        70         80         90        100        110        120 
ERKLRSEIIS AVKRDLSKNK IVIVDSLNYI KGFRYQLHCE VKNLSTTFCV IQTLCPPETI 

       130        140        150        160        170        180 
FEWNKTSNPN PWEPELLNQL IQRYEEPNSS NRWDSPLFAI LTPQDNITDY IDDICKVVFQ 

       190        200        210        220        230        240 
TSKSAKNSGH NDPLSKGLQK PNSATVLKPA SQSNFIQVLD IETSKIIKTI MNHIKSLTSI 

       250        260        270        280        290        300 
GGVSNGTRVI VSEGITDIND DGCFFVDLPI GNVVTLAQLQ RLKRQFINFN KLRDIDQDRI 

       310 
GPLFADYLNK NLN 

« Hide

References

« Hide 'large scale' references
[1]"Two Saccharomyces cerevisiae genes which control sensitivity to G1 arrest induced by Kluyveromyces lactis toxin."
Butler A.R., White J.H., Folawiyo Y., Edlin A., Gardiner D., Stark M.J.R.
Mol. Cell. Biol. 14:6306-6316(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION.
[2]"Complete DNA sequence of yeast chromosome XI."
Dujon B., Alexandraki D., Andre B., Ansorge W., Baladron V., Ballesta J.P.G., Banrevi A., Bolle P.-A., Bolotin-Fukuhara M., Bossier P., Bou G., Boyer J., Buitrago M.J., Cheret G., Colleaux L., Daignan-Fornier B., del Rey F., Dion C. expand/collapse author list , Domdey H., Duesterhoeft A., Duesterhus S., Entian K.-D., Erfle H., Esteban P.F., Feldmann H., Fernandes L., Fobo G.M., Fritz C., Fukuhara H., Gabel C., Gaillon L., Garcia-Cantalejo J.M., Garcia-Ramirez J.J., Gent M.E., Ghazvini M., Goffeau A., Gonzalez A., Grothues D., Guerreiro P., Hegemann J.H., Hewitt N., Hilger F., Hollenberg C.P., Horaitis O., Indge K.J., Jacquier A., James C.M., Jauniaux J.-C., Jimenez A., Keuchel H., Kirchrath L., Kleine K., Koetter P., Legrain P., Liebl S., Louis E.J., Maia e Silva A., Marck C., Monnier A.-L., Moestl D., Mueller S., Obermaier B., Oliver S.G., Pallier C., Pascolo S., Pfeiffer F., Philippsen P., Planta R.J., Pohl F.M., Pohl T.M., Poehlmann R., Portetelle D., Purnelle B., Puzos V., Ramezani Rad M., Rasmussen S.W., Remacha M.A., Revuelta J.L., Richard G.-F., Rieger M., Rodrigues-Pousada C., Rose M., Rupp T., Santos M.A., Schwager C., Sensen C., Skala J., Soares H., Sor F., Stegemann J., Tettelin H., Thierry A., Tzermia M., Urrestarazu L.A., van Dyck L., van Vliet-Reedijk J.C., Valens M., Vandenbol M., Vilela C., Vissers S., von Wettstein D., Voss H., Wiemann S., Xu G., Zimmermann J., Haasemann M., Becker I., Mewes H.-W.
Nature 369:371-378(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[3]"The reference genome sequence of Saccharomyces cerevisiae: Then and now."
Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R., Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S., Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.
G3 (Bethesda) 4:389-398(2014) [PubMed] [Europe PMC] [Abstract]
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[4]"The DNA sequence analysis of the HAP4-LAP4 region on chromosome XI of Saccharomyces cerevisiae suggests the presence of a second aspartate aminotransferase gene in yeast."
Cheret G., Pallier C., Valens M., Daignan-Fornier B., Fukuhara H., Bolotin-Fukuhara M., Sor F.
Yeast 9:1259-1265(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-75.
[5]"Sequence of a 10.7 kb segment of yeast chromosome XI identifies the APN1 and the BAF1 loci and reveals one tRNA gene and several new open reading frames including homologs to RAD2 and kinases."
Jacquier A., Legrain P., Dujon B.
Yeast 8:121-132(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 75-313.
[6]"Similarity between the transcriptional silencer binding proteins ABF1 and RAP1."
Diffley J.F.X., Stillman B.
Science 246:1034-1038(1989) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 103-313.
[7]"Saccharomyces cerevisiae Elongator mutations confer resistance to the Kluyveromyces lactis zymocin."
Frohloff F., Fichtner L., Jablonowski D., Breunig K.D., Schaffrath R.
EMBO J. 20:1993-2003(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, INTERACTION WITH THE ELONGATOR COMPLEX.
[8]"Molecular analysis of KTI12/TOT4, a Saccharomyces cerevisiae gene required for Kluyveromyces lactis zymocin action."
Fichtner L., Frohloff F., Buerkner K., Larsen M., Breunig K.D., Schaffrath R.
Mol. Microbiol. 43:783-791(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, INTERACTION WITH THE ELONGATOR COMPLEX.
[9]"Protein interactions within Saccharomyces cerevisiae Elongator, a complex essential for Kluyveromyces lactis zymocicity."
Fichtner L., Frohloff F., Jablonowski D., Stark M.J.R., Schaffrath R.
Mol. Microbiol. 45:817-826(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH THE ELONGATOR COMPLEX.
[10]"Subunit communications crucial for the functional integrity of the yeast RNA polymerase II elongator (gamma-toxin target (TOT)) complex."
Frohloff F., Jablonowski D., Fichtner L., Schaffrath R.
J. Biol. Chem. 278:956-961(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, INTERACTION WITH THE ELONGATOR COMPLEX.
[11]"Global analysis of protein localization in budding yeast."
Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K.
Nature 425:686-691(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[12]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[13]"The yeast elongator histone acetylase requires Sit4-dependent dephosphorylation for toxin-target capacity."
Jablonowski D., Fichtner L., Stark M.J.R., Schaffrath R.
Mol. Biol. Cell 15:1459-1469(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, INTERACTION WITH THE ELONGATOR COMPLEX.
[14]"Physical and functional interaction between Elongator and the chromatin-associated Kti12 protein."
Petrakis T.G., Soegaard T.M.M., Erdjument-Bromage H., Tempst P., Svejstrup J.Q.
J. Biol. Chem. 280:19454-19460(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, INTERACTION WITH THE ELONGATOR COMPLEX, CHROMATIN BINDING.
[15]"An early step in wobble uridine tRNA modification requires the Elongator complex."
Huang B., Johansson M.J.O., Bystroem A.S.
RNA 11:424-436(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X77511 Genomic DNA. Translation: CAA54646.1.
X71133 Genomic DNA. Translation: CAA50447.1.
S93804 Genomic DNA. Translation: AAB22001.1.
Z28110 Genomic DNA. Translation: CAA81950.1.
M29067 Genomic DNA. Translation: AAA66312.1.
BK006944 Genomic DNA. Translation: DAA09047.1.
PIRS37937.
RefSeqNP_012812.1. NM_001179676.1.

3D structure databases

ProteinModelPortalP34253.
SMRP34253. Positions 3-183.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid34023. 104 interactions.
DIPDIP-1856N.
IntActP34253. 2 interactions.
MINTMINT-408483.
STRING4932.YKL110C.

Proteomic databases

MaxQBP34253.
PaxDbP34253.
PeptideAtlasP34253.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYKL110C; YKL110C; YKL110C.
GeneID853750.
KEGGsce:YKL110C.

Organism-specific databases

CYGDYKL110c.
SGDS000001593. KTI12.

Phylogenomic databases

eggNOGCOG4088.
GeneTreeENSGT00390000002443.
HOGENOMHOG000247843.
KOK15456.
OMAPCSGKTR.
OrthoDBEOG7RBZKK.

Enzyme and pathway databases

BioCycYEAST:G3O-31896-MONOMER.

Gene expression databases

GenevestigatorP34253.

Family and domain databases

Gene3D3.40.50.300. 1 hit.
InterProIPR013641. Chromatin_KTI12.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamPF08433. KTI12. 1 hit.
[Graphical view]
SUPFAMSSF52540. SSF52540. 1 hit.
ProtoNetSearch...

Other

NextBio974813.
PROP34253.

Entry information

Entry nameKTI12_YEAST
AccessionPrimary (citable) accession number: P34253
Secondary accession number(s): D6VXH7, Q05155
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: June 1, 1994
Last modified: July 9, 2014
This is version 108 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome XI

Yeast (Saccharomyces cerevisiae) chromosome XI: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families