Protein KTI12 - Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

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P34253 (KTI12_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 96. History...

Clusters with 100%, 90%, 50% identity |

Documents (3) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Protein KTI12

Alternative name(s):
Gamma-toxin target protein 4
Killer toxin insensitivity protein 12

Gene names

Name:	KTI12
Ordered Locus Names:	YKL110C
ORF Names:	YKL446, YKL500

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces ›

Protein attributes

Sequence length	313 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Elongator-associated factor that is not a structural subunit but rather transiently contacts Elongator, presumably to promote its interaction with elongation competent pol II. Regulates Elongator complex gamma-toxin target (TOT) activity, resulting in G1 block by Kluyveromyces lactis toxin zymocin (pGKL1 killer toxin), probably through regulation of the Elongator IKI3 subunit phosphorylation state. Required for an early step in synthesis of 5-methoxycarbonylmethyl (mcm5) and 5-carbamoylmethyl (ncm5) groups present on uridines at the wobble position in tRNA. Ref.1 Ref.7 Ref.8 Ref.10 Ref.13 Ref.14 Ref.15
Subunit structure	Interacts with the RNA polymerase II elongator complex and RNA polymerase II. Ref.7 Ref.8 Ref.9 Ref.10 Ref.13 Ref.14
Subcellular location	Cytoplasm. Nucleus Ref.11.
Miscellaneous	Present with 5260 molecules/cell in log phase SD medium.
Sequence similarities	Belongs to the KTI12 family.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Cytoplasm Nucleus
Ligand	ATP-binding Nucleotide-binding
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cell growth Inferred from physical interaction Ref.9. Source: SGD regulation of transcription from RNA polymerase II promoter Inferred from genetic interaction Ref.14. Source: SGD tRNA wobble uridine modification Inferred from mutant phenotype PubMed 18755837. Source: SGD transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	cytoplasm Inferred from direct assay Ref.9. Source: SGD nucleus Inferred from direct assay Ref.9. Source: SGD
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW chromatin binding Inferred from direct assay Ref.14. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 313

313

Protein KTI12

PRO_0000084335

Regions

Nucleotide binding

8 – 15

ATP Potential

Experimental info

Sequence conflict

103

N → D in AAA66312. Ref.5

Sequence conflict

150

S → N in AAA66312. Ref.5

Sequences

Sequence

Length

Mass (Da)

Tools

P34253 [UniParc].

Last modified June 1, 1994. Version 2.
Checksum: 15A9534087A24AE6
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FASTA

313

35,322

        10         20         30         40         50         60 
MPLVLFTGYP CSGKTTLAKH LVQLLQSKID ATPSLSKYSI TYHSDESLGI KHSDYITSQD 

        70         80         90        100        110        120 
ERKLRSEIIS AVKRDLSKNK IVIVDSLNYI KGFRYQLHCE VKNLSTTFCV IQTLCPPETI 

       130        140        150        160        170        180 
FEWNKTSNPN PWEPELLNQL IQRYEEPNSS NRWDSPLFAI LTPQDNITDY IDDICKVVFQ 

       190        200        210        220        230        240 
TSKSAKNSGH NDPLSKGLQK PNSATVLKPA SQSNFIQVLD IETSKIIKTI MNHIKSLTSI 

       250        260        270        280        290        300 
GGVSNGTRVI VSEGITDIND DGCFFVDLPI GNVVTLAQLQ RLKRQFINFN KLRDIDQDRI 

       310 
GPLFADYLNK NLN

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Two Saccharomyces cerevisiae genes which control sensitivity to G1 arrest induced by Kluyveromyces lactis toxin." Butler A.R., White J.H., Folawiyo Y., Edlin A., Gardiner D., Stark M.J.R. Mol. Cell. Biol. 14:6306-6316(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION.
[2]	"Complete DNA sequence of yeast chromosome XI." Dujon B., Alexandraki D., Andre B., Ansorge W., Baladron V., Ballesta J.P.G., Banrevi A., Bolle P.-A., Bolotin-Fukuhara M., Bossier P., Bou G., Boyer J., Buitrago M.J., Cheret G., Colleaux L., Daignan-Fornier B., del Rey F., Dion C. Mewes H.-W. Nature 369:371-378(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[3]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[4]	"The DNA sequence analysis of the HAP4-LAP4 region on chromosome XI of Saccharomyces cerevisiae suggests the presence of a second aspartate aminotransferase gene in yeast." Cheret G., Pallier C., Valens M., Daignan-Fornier B., Fukuhara H., Bolotin-Fukuhara M., Sor F. Yeast 9:1259-1265(1993) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-75.
[5]	"Sequence of a 10.7 kb segment of yeast chromosome XI identifies the APN1 and the BAF1 loci and reveals one tRNA gene and several new open reading frames including homologs to RAD2 and kinases." Jacquier A., Legrain P., Dujon B. Yeast 8:121-132(1992) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 75-313.
[6]	"Similarity between the transcriptional silencer binding proteins ABF1 and RAP1." Diffley J.F.X., Stillman B. Science 246:1034-1038(1989) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 103-313.
[7]	"Saccharomyces cerevisiae Elongator mutations confer resistance to the Kluyveromyces lactis zymocin." Frohloff F., Fichtner L., Jablonowski D., Breunig K.D., Schaffrath R. EMBO J. 20:1993-2003(2001) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, INTERACTION WITH THE ELONGATOR COMPLEX.
[8]	"Molecular analysis of KTI12/TOT4, a Saccharomyces cerevisiae gene required for Kluyveromyces lactis zymocin action." Fichtner L., Frohloff F., Buerkner K., Larsen M., Breunig K.D., Schaffrath R. Mol. Microbiol. 43:783-791(2002) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, INTERACTION WITH THE ELONGATOR COMPLEX.
[9]	"Protein interactions within Saccharomyces cerevisiae Elongator, a complex essential for Kluyveromyces lactis zymocicity." Fichtner L., Frohloff F., Jablonowski D., Stark M.J.R., Schaffrath R. Mol. Microbiol. 45:817-826(2002) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH THE ELONGATOR COMPLEX.
[10]	"Subunit communications crucial for the functional integrity of the yeast RNA polymerase II elongator (gamma-toxin target (TOT)) complex." Frohloff F., Jablonowski D., Fichtner L., Schaffrath R. J. Biol. Chem. 278:956-961(2003) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, INTERACTION WITH THE ELONGATOR COMPLEX.
[11]	"Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[12]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[13]	"The yeast elongator histone acetylase requires Sit4-dependent dephosphorylation for toxin-target capacity." Jablonowski D., Fichtner L., Stark M.J.R., Schaffrath R. Mol. Biol. Cell 15:1459-1469(2004) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, INTERACTION WITH THE ELONGATOR COMPLEX.
[14]	"Physical and functional interaction between Elongator and the chromatin-associated Kti12 protein." Petrakis T.G., Soegaard T.M.M., Erdjument-Bromage H., Tempst P., Svejstrup J.Q. J. Biol. Chem. 280:19454-19460(2005) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, INTERACTION WITH THE ELONGATOR COMPLEX, CHROMATIN BINDING.
[15]	"An early step in wobble uridine tRNA modification requires the Elongator complex." Huang B., Johansson M.J.O., Bystroem A.S. RNA 11:424-436(2005) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X77511 Genomic DNA. Translation: CAA54646.1. X71133 Genomic DNA. Translation: CAA50447.1. S93804 Genomic DNA. Translation: AAB22001.1. Z28110 Genomic DNA. Translation: CAA81950.1. M29067 Genomic DNA. Translation: AAA66312.1. BK006944 Genomic DNA. Translation: DAA09047.1.
PIR	S37937.
RefSeq	NP_012812.1. NM_001179676.1.
3D structure databases
ProteinModelPortal	P34253.
SMR	P34253. Positions 3-183.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-1856N.
IntAct	P34253. 2 interactions.
MINT	MINT-408483.
STRING	4932.YKL110C.
Proteomic databases
PaxDb	P34253.
PeptideAtlas	P34253.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YKL110C; YKL110C; YKL110C.
GeneID	853750.
KEGG	sce:YKL110C.
Organism-specific databases
CYGD	YKL110c.
SGD	S000001593. KTI12.
Phylogenomic databases
eggNOG	COG4088.
GeneTree	ENSGT00390000002443.
HOGENOM	HOG000247843.
KO	K15456.
OMA	IQRYEEP.
OrthoDB	EOG40P7GH.
Gene expression databases
Genevestigator	P34253.
GermOnline	YKL110C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR013641. Chromatin_KTI12. [Graphical view]
Pfam	PF08433. KTI12. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	974813.

Entry information

Entry name

KTI12_YEAST

Accession

Primary (citable) accession number: P34253
Secondary accession number(s): D6VXH7, Q05155

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1994
Last sequence update:	June 1, 1994
Last modified:	May 1, 2013
This is version 96 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program

Relevant documents

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

Yeast chromosome XI

Yeast (Saccharomyces cerevisiae) chromosome XI: entries and gene names

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Experimental info

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents