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P34253

- KTI12_YEAST

UniProt

P34253 - KTI12_YEAST

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Protein

Protein KTI12

Gene

KTI12

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Elongator-associated factor that is not a structural subunit but rather transiently contacts Elongator, presumably to promote its interaction with elongation competent pol II. Regulates Elongator complex gamma-toxin target (TOT) activity, resulting in G1 block by Kluyveromyces lactis toxin zymocin (pGKL1 killer toxin), probably through regulation of the Elongator IKI3 subunit phosphorylation state. Required for an early step in synthesis of 5-methoxycarbonylmethyl (mcm5) and 5-carbamoylmethyl (ncm5) groups present on uridines at the wobble position in tRNA.7 Publications

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi8 – 158ATPSequence Analysis

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. chromatin binding Source: SGD

GO - Biological processi

  1. cell growth Source: SGD
  2. regulation of transcription from RNA polymerase II promoter Source: SGD
  3. transcription, DNA-templated Source: UniProtKB-KW
  4. tRNA wobble uridine modification Source: SGD
Complete GO annotation...

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-31896-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Protein KTI12
Alternative name(s):
Gamma-toxin target protein 4
Killer toxin insensitivity protein 12
Gene namesi
Name:KTI12
Ordered Locus Names:YKL110C
ORF Names:YKL446, YKL500
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome XI

Organism-specific databases

CYGDiYKL110c.
SGDiS000001593. KTI12.

Subcellular locationi

Cytoplasm 1 Publication. Nucleus 1 Publication

GO - Cellular componenti

  1. cytoplasm Source: SGD
  2. nucleus Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 313313Protein KTI12PRO_0000084335Add
BLAST

Proteomic databases

MaxQBiP34253.
PaxDbiP34253.
PeptideAtlasiP34253.

Expressioni

Gene expression databases

GenevestigatoriP34253.

Interactioni

Subunit structurei

Interacts with the RNA polymerase II elongator complex and RNA polymerase II.6 Publications

Protein-protein interaction databases

BioGridi34023. 106 interactions.
DIPiDIP-1856N.
IntActiP34253. 2 interactions.
MINTiMINT-408483.
STRINGi4932.YKL110C.

Structurei

3D structure databases

ProteinModelPortaliP34253.
SMRiP34253. Positions 3-183.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the KTI12 family.Curated

Phylogenomic databases

eggNOGiCOG4088.
GeneTreeiENSGT00390000002443.
HOGENOMiHOG000247843.
InParanoidiP34253.
KOiK15456.
OMAiPCSGKTR.
OrthoDBiEOG7RBZKK.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR013641. Chromatin_KTI12.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF08433. KTI12. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.

Sequencei

Sequence statusi: Complete.

P34253-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MPLVLFTGYP CSGKTTLAKH LVQLLQSKID ATPSLSKYSI TYHSDESLGI
60 70 80 90 100
KHSDYITSQD ERKLRSEIIS AVKRDLSKNK IVIVDSLNYI KGFRYQLHCE
110 120 130 140 150
VKNLSTTFCV IQTLCPPETI FEWNKTSNPN PWEPELLNQL IQRYEEPNSS
160 170 180 190 200
NRWDSPLFAI LTPQDNITDY IDDICKVVFQ TSKSAKNSGH NDPLSKGLQK
210 220 230 240 250
PNSATVLKPA SQSNFIQVLD IETSKIIKTI MNHIKSLTSI GGVSNGTRVI
260 270 280 290 300
VSEGITDIND DGCFFVDLPI GNVVTLAQLQ RLKRQFINFN KLRDIDQDRI
310
GPLFADYLNK NLN
Length:313
Mass (Da):35,322
Last modified:June 1, 1994 - v2
Checksum:i15A9534087A24AE6
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti103 – 1031N → D in AAA66312. (PubMed:1561835)Curated
Sequence conflicti150 – 1501S → N in AAA66312. (PubMed:1561835)Curated

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X77511 Genomic DNA. Translation: CAA54646.1.
X71133 Genomic DNA. Translation: CAA50447.1.
S93804 Genomic DNA. Translation: AAB22001.1.
Z28110 Genomic DNA. Translation: CAA81950.1.
M29067 Genomic DNA. Translation: AAA66312.1.
BK006944 Genomic DNA. Translation: DAA09047.1.
PIRiS37937.
RefSeqiNP_012812.1. NM_001179676.1.

Genome annotation databases

EnsemblFungiiYKL110C; YKL110C; YKL110C.
GeneIDi853750.
KEGGisce:YKL110C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X77511 Genomic DNA. Translation: CAA54646.1 .
X71133 Genomic DNA. Translation: CAA50447.1 .
S93804 Genomic DNA. Translation: AAB22001.1 .
Z28110 Genomic DNA. Translation: CAA81950.1 .
M29067 Genomic DNA. Translation: AAA66312.1 .
BK006944 Genomic DNA. Translation: DAA09047.1 .
PIRi S37937.
RefSeqi NP_012812.1. NM_001179676.1.

3D structure databases

ProteinModelPortali P34253.
SMRi P34253. Positions 3-183.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 34023. 106 interactions.
DIPi DIP-1856N.
IntActi P34253. 2 interactions.
MINTi MINT-408483.
STRINGi 4932.YKL110C.

Proteomic databases

MaxQBi P34253.
PaxDbi P34253.
PeptideAtlasi P34253.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YKL110C ; YKL110C ; YKL110C .
GeneIDi 853750.
KEGGi sce:YKL110C.

Organism-specific databases

CYGDi YKL110c.
SGDi S000001593. KTI12.

Phylogenomic databases

eggNOGi COG4088.
GeneTreei ENSGT00390000002443.
HOGENOMi HOG000247843.
InParanoidi P34253.
KOi K15456.
OMAi PCSGKTR.
OrthoDBi EOG7RBZKK.

Enzyme and pathway databases

BioCyci YEAST:G3O-31896-MONOMER.

Miscellaneous databases

NextBioi 974813.
PROi P34253.

Gene expression databases

Genevestigatori P34253.

Family and domain databases

Gene3Di 3.40.50.300. 1 hit.
InterProi IPR013641. Chromatin_KTI12.
IPR027417. P-loop_NTPase.
[Graphical view ]
Pfami PF08433. KTI12. 1 hit.
[Graphical view ]
SUPFAMi SSF52540. SSF52540. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Two Saccharomyces cerevisiae genes which control sensitivity to G1 arrest induced by Kluyveromyces lactis toxin."
    Butler A.R., White J.H., Folawiyo Y., Edlin A., Gardiner D., Stark M.J.R.
    Mol. Cell. Biol. 14:6306-6316(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION.
  2. "Complete DNA sequence of yeast chromosome XI."
    Dujon B., Alexandraki D., Andre B., Ansorge W., Baladron V., Ballesta J.P.G., Banrevi A., Bolle P.-A., Bolotin-Fukuhara M., Bossier P., Bou G., Boyer J., Buitrago M.J., Cheret G., Colleaux L., Daignan-Fornier B., del Rey F., Dion C.
    , Domdey H., Duesterhoeft A., Duesterhus S., Entian K.-D., Erfle H., Esteban P.F., Feldmann H., Fernandes L., Fobo G.M., Fritz C., Fukuhara H., Gabel C., Gaillon L., Garcia-Cantalejo J.M., Garcia-Ramirez J.J., Gent M.E., Ghazvini M., Goffeau A., Gonzalez A., Grothues D., Guerreiro P., Hegemann J.H., Hewitt N., Hilger F., Hollenberg C.P., Horaitis O., Indge K.J., Jacquier A., James C.M., Jauniaux J.-C., Jimenez A., Keuchel H., Kirchrath L., Kleine K., Koetter P., Legrain P., Liebl S., Louis E.J., Maia e Silva A., Marck C., Monnier A.-L., Moestl D., Mueller S., Obermaier B., Oliver S.G., Pallier C., Pascolo S., Pfeiffer F., Philippsen P., Planta R.J., Pohl F.M., Pohl T.M., Poehlmann R., Portetelle D., Purnelle B., Puzos V., Ramezani Rad M., Rasmussen S.W., Remacha M.A., Revuelta J.L., Richard G.-F., Rieger M., Rodrigues-Pousada C., Rose M., Rupp T., Santos M.A., Schwager C., Sensen C., Skala J., Soares H., Sor F., Stegemann J., Tettelin H., Thierry A., Tzermia M., Urrestarazu L.A., van Dyck L., van Vliet-Reedijk J.C., Valens M., Vandenbol M., Vilela C., Vissers S., von Wettstein D., Voss H., Wiemann S., Xu G., Zimmermann J., Haasemann M., Becker I., Mewes H.-W.
    Nature 369:371-378(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  4. "The DNA sequence analysis of the HAP4-LAP4 region on chromosome XI of Saccharomyces cerevisiae suggests the presence of a second aspartate aminotransferase gene in yeast."
    Cheret G., Pallier C., Valens M., Daignan-Fornier B., Fukuhara H., Bolotin-Fukuhara M., Sor F.
    Yeast 9:1259-1265(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-75.
  5. "Sequence of a 10.7 kb segment of yeast chromosome XI identifies the APN1 and the BAF1 loci and reveals one tRNA gene and several new open reading frames including homologs to RAD2 and kinases."
    Jacquier A., Legrain P., Dujon B.
    Yeast 8:121-132(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 75-313.
  6. "Similarity between the transcriptional silencer binding proteins ABF1 and RAP1."
    Diffley J.F.X., Stillman B.
    Science 246:1034-1038(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 103-313.
  7. "Saccharomyces cerevisiae Elongator mutations confer resistance to the Kluyveromyces lactis zymocin."
    Frohloff F., Fichtner L., Jablonowski D., Breunig K.D., Schaffrath R.
    EMBO J. 20:1993-2003(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH THE ELONGATOR COMPLEX.
  8. "Molecular analysis of KTI12/TOT4, a Saccharomyces cerevisiae gene required for Kluyveromyces lactis zymocin action."
    Fichtner L., Frohloff F., Buerkner K., Larsen M., Breunig K.D., Schaffrath R.
    Mol. Microbiol. 43:783-791(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH THE ELONGATOR COMPLEX.
  9. "Protein interactions within Saccharomyces cerevisiae Elongator, a complex essential for Kluyveromyces lactis zymocicity."
    Fichtner L., Frohloff F., Jablonowski D., Stark M.J.R., Schaffrath R.
    Mol. Microbiol. 45:817-826(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH THE ELONGATOR COMPLEX.
  10. "Subunit communications crucial for the functional integrity of the yeast RNA polymerase II elongator (gamma-toxin target (TOT)) complex."
    Frohloff F., Jablonowski D., Fichtner L., Schaffrath R.
    J. Biol. Chem. 278:956-961(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH THE ELONGATOR COMPLEX.
  11. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
  12. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  13. "The yeast elongator histone acetylase requires Sit4-dependent dephosphorylation for toxin-target capacity."
    Jablonowski D., Fichtner L., Stark M.J.R., Schaffrath R.
    Mol. Biol. Cell 15:1459-1469(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH THE ELONGATOR COMPLEX.
  14. "Physical and functional interaction between Elongator and the chromatin-associated Kti12 protein."
    Petrakis T.G., Soegaard T.M.M., Erdjument-Bromage H., Tempst P., Svejstrup J.Q.
    J. Biol. Chem. 280:19454-19460(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH THE ELONGATOR COMPLEX, CHROMATIN BINDING.
  15. "An early step in wobble uridine tRNA modification requires the Elongator complex."
    Huang B., Johansson M.J.O., Bystroem A.S.
    RNA 11:424-436(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.

Entry informationi

Entry nameiKTI12_YEAST
AccessioniPrimary (citable) accession number: P34253
Secondary accession number(s): D6VXH7, Q05155
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: June 1, 1994
Last modified: October 29, 2014
This is version 110 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 5260 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XI
    Yeast (Saccharomyces cerevisiae) chromosome XI: entries and gene names

External Data

Dasty 3