P34252 (SLD2_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
DNA replication regulator SLD2

Alternative name(s):
DNA replication and checkpoint protein 1

Gene names

Name:	SLD2
Synonyms:	DRC1
Ordered Locus Names:	YKL108W
ORF Names:	YKL463

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	453 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Has a role in the intiation of DNA replication. Required at S-phase checkpoint. Also required for the proper activation of RAD53 in response to DNA damage and replication blocks. Ref.4 Ref.5 Ref.6
Subunit structure	Interacts with DPB11. Ref.4 Ref.5 Ref.9
Subcellular location	Nucleus By similarity.
Post-translational modification	Phosphorylated by CDC28 at the onset of S-phase. This phosphorylation, specifically phosphorylation of Thr-84 promotes interaction with DPB11 leading to DNA replication. Dephosphorylated by CDC14. Ref.6 Ref.8 Ref.9 Ref.10
Miscellaneous	Present with 656 molecules/cell in log phase SD medium. Ref.7
Sequence similarities	Belongs to the SLD2 family.

Ontologies

Keywords
Biological process	Cell cycle DNA replication
Cellular component	Nucleus
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	DNA replication preinitiation complex assembly Inferred from mutant phenotype. Source: SGD cell cycle Inferred from electronic annotation. Source: UniProtKB-KW double-strand break repair via break-induced replication Inferred from mutant phenotype. Source: SGD mitotic cell cycle DNA replication checkpoint Inferred from mutant phenotype Ref.5. Source: SGD
Cellular component	DNA replication preinitiation complex Inferred from direct assay. Source: SGD
Molecular function	DNA replication origin binding Inferred from mutant phenotype. Source: SGD DNA strand annealing activity Inferred from mutant phenotype. Source: SGD
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
DPB11	P47027	1	EBI-26824,EBI-25984

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 453	453	DNA replication regulator SLD2		PRO_0000071952
Amino acid modifications
	Modified residue	84	1	Phosphothreonine; by CDC28 Ref.9
Experimental info
	Mutagenesis	84	1	T → A: No interaction with DPB11. Ref.9
	Mutagenesis	100	1	S → A: No interaction with DPB11. Ref.9
	Mutagenesis	208	1	S → A: Normal interaction with DPB11. Ref.9
	Mutagenesis	241	1	T → A: Reduced interaction with DPB11. Ref.9

Sequences

	Sequence		Length	Mass (Da)
	P34252 [UniParc]. Last modified February 1, 1994. Version 1. Checksum: 52B2969C97AAEAC8	FASTA	453	52,272
	10 20 30 40 50 60 MYSFELDKLK IELKTWEHDF IDKNKREPTR DDIKSLRTVR QMYKQYSTLK KKQSLQRQKV 70 80 90 100 110 120 DTQESVELPA HKKDHDEVVE IGPTPQVYGK AISIFDMNLS PIKPIYMTFT NNIDVNNDNS 130 140 150 160 170 180 KTISNESSPR KTILLKSSPA DRTLVAEPIS SVKRQLNFQM LNASSTRTPT SSPCKNRNGK 190 200 210 220 230 240 LVEIKKCSPT INPPLESGKP SGYYGPNSPL KLDEENIHLN ISLNSSTKRR LQIAYPSLQK 250 260 270 280 290 300 TPSKDQADIS TSFSPSPLIR RPLTKSLIEL AREHTEIVKE FGVLQEEDIE EEEEGEEGEN 310 320 330 340 350 360 GYDEKNHEDD FGLEDELIRP KVVKDIFQED DDNDDSQARE DTFIRKRPKR RKVIRRLRDN 370 380 390 400 410 420 DPETETAGFE RDVHKELVKL KRRKVAEFLG STSQISDTEF EHDPEASSGV VSSEQKPTAK 430 440 450 RKGRKKYNLV SNNFRRLKLP KKNRFSNGRW GRR

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The DNA sequence analysis of the HAP4-LAP4 region on chromosome XI of Saccharomyces cerevisiae suggests the presence of a second aspartate aminotransferase gene in yeast." Cheret G., Pallier C., Valens M., Daignan-Fornier B., Fukuhara H., Bolotin-Fukuhara M., Sor F. Yeast 9:1259-1265(1993) [PubMed: 8109175] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[2]	"Complete DNA sequence of yeast chromosome XI." Dujon B., Alexandraki D., Andre B., Ansorge W., Baladron V., Ballesta J.P.G., Banrevi A., Bolle P.-A., Bolotin-Fukuhara M., Bossier P., Bou G., Boyer J., Buitrago M.J., Cheret G., Colleaux L., Daignan-Fornier B., del Rey F., Dion C. , Domdey H., Duesterhoeft A., Duesterhus S., Entian K.-D., Erfle H., Esteban P.F., Feldmann H., Fernandes L., Fobo G.M., Fritz C., Fukuhara H., Gabel C., Gaillon L., Garcia-Cantalejo J.M., Garcia-Ramirez J.J., Gent M.E., Ghazvini M., Goffeau A., Gonzalez A., Grothues D., Guerreiro P., Hegemann J.H., Hewitt N., Hilger F., Hollenberg C.P., Horaitis O., Indge K.J., Jacquier A., James C.M., Jauniaux J.-C., Jimenez A., Keuchel H., Kirchrath L., Kleine K., Koetter P., Legrain P., Liebl S., Louis E.J., Maia e Silva A., Marck C., Monnier A.-L., Moestl D., Mueller S., Obermaier B., Oliver S.G., Pallier C., Pascolo S., Pfeiffer F., Philippsen P., Planta R.J., Pohl F.M., Pohl T.M., Poehlmann R., Portetelle D., Purnelle B., Puzos V., Ramezani Rad M., Rasmussen S.W., Remacha M.A., Revuelta J.L., Richard G.-F., Rieger M., Rodrigues-Pousada C., Rose M., Rupp T., Santos M.A., Schwager C., Sensen C., Skala J., Soares H., Sor F., Stegemann J., Tettelin H., Thierry A., Tzermia M., Urrestarazu L.A., van Dyck L., van Vliet-Reedijk J.C., Valens M., Vandenbol M., Vilela C., Vissers S., von Wettstein D., Voss H., Wiemann S., Xu G., Zimmermann J., Haasemann M., Becker I., Mewes H.-W. Nature 369:371-378(1994) [PubMed: 8196765] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[3]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[4]	"Sld2, which interacts with Dpb11 in Saccharomyces cerevisiae, is required for chromosomal DNA replication." Kamimura Y., Masumoto H., Sugino A., Araki H. Mol. Cell. Biol. 18:6102-6109(1998) [PubMed: 9742127] [Abstract] Cited for: FUNCTION, INTERACTION WITH DPB11.
[5]	"DRC1, DNA replication and checkpoint protein 1, functions with DPB11 to control DNA replication and the S-phase checkpoint in Saccharomyces cerevisiae." Wang H., Elledge S.J. Proc. Natl. Acad. Sci. U.S.A. 96:3824-3829(1999) [PubMed: 10097122] [Abstract] Cited for: FUNCTION, INTERACTION WITH DPB11.
[6]	"S-Cdk-dependent phosphorylation of Sld2 essential for chromosomal DNA replication in budding yeast." Masumoto H., Muramatsu S., Kamimura Y., Araki H. Nature 415:651-655(2002) [PubMed: 11807498] [Abstract] Cited for: FUNCTION, PHOSPHORYLATION BY CDC28.
[7]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[8]	"Targets of the cyclin-dependent kinase Cdk1." Ubersax J.A., Woodbury E.L., Quang P.N., Paraz M., Blethrow J.D., Shah K., Shokat K.M., Morgan D.O. Nature 425:859-864(2003) [PubMed: 14574415] [Abstract] Cited for: PHOSPHORYLATION BY CDC28.
[9]	"A CDK-catalysed regulatory phosphorylation for formation of the DNA replication complex Sld2-Dpb11." Tak Y.-S., Tanaka Y., Endo S., Kamimura Y., Araki H. EMBO J. 25:1987-1996(2006) [PubMed: 16619031] [Abstract] Cited for: INTERACTION WITH DPB11, PHOSPHORYLATION AT THR-84, MUTAGENESIS OF THR-84; SER-100; SER-208 AND THR-241.
[10]	"Novel role for Cdc14 sequestration: Cdc14 dephosphorylates factors that promote DNA replication." Bloom J., Cross F.R. Mol. Cell. Biol. 27:842-853(2007) [PubMed: 17116692] [Abstract] Cited for: DEPHOSPHORYLATION BY CDC14.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X71133 Genomic DNA. Translation: CAA50449.1. Z28108 Genomic DNA. Translation: CAA81948.1. BK006944 Genomic DNA. Translation: DAA09049.1.
PIR	S37935.
RefSeq	NP_012814.1. NM_001179674.1.
3D structure databases
ProteinModelPortal	P34252.
SMR	P34252. Positions 5-55.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-5880N.
IntAct	P34252. 15 interactions.
MINT	MINT-673761.
STRING	P34252.
Proteomic databases
PeptideAtlas	P34252.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YKL108W; YKL108W; YKL108W.
GeneID	853752.
KEGG	sce:YKL108W.
NMPDR	fig|4932.3.peg.3795.
Organism-specific databases
CYGD	YKL108w.
SGD	S000001591. SLD2.
Phylogenomic databases
eggNOG	fuNOG10961.
HOGENOM	HBG202304.
OMA	MHEEDET.
OrthoDB	EOG4R7ZMH.
Gene expression databases
ArrayExpress	P34252.
Genevestigator	P34252.
GermOnline	YKL108W. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR021110. DNA_rep_checkpnt_protein. [Graphical view]
KO	K10729.
Pfam	PF11719. Drc1-Sld2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	974819.

Entry information

Entry name

SLD2_YEAST

Accession

Primary (citable) accession number: P34252
Secondary accession number(s): D6VXH9

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1994
Last sequence update:	February 1, 1994
Last modified:	January 25, 2012
This is version 84 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program