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P34248 (YKK0_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified April 3, 2013. Version 92. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data text xml rdf/xml gff fasta
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to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Probable intramembrane protease YKL100C
EC=3.4.23.-
Gene names
Ordered Locus Names:YKL100C
ORF Names:YKL450
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces ›

Protein attributes

Sequence length587 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

May act as intramembrane protease By similarity.

Subcellular location

Membrane; Multi-pass membrane protein By similarity. Endoplasmic reticulum membrane; Multi-pass membrane protein By similarity.

Domain

The PAL motif is required for normal active site conformation By similarity.

Sequence similarities

Belongs to the peptidase A22B family.

Ontologies

Keywords
   Cellular componentEndoplasmic reticulum
Membrane
   DomainTransmembrane
Transmembrane helix
   Molecular functionHydrolase
Protease
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processproteolysis

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular_componentendoplasmic reticulum membrane

Inferred from electronic annotation. Source: UniProtKB-SubCell

integral to membrane

Inferred from sequence model PubMed 12192589. Source: SGD

   Molecular_functionaspartic-type endopeptidase activity

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 587587Probable intramembrane protease YKL100C
PRO_0000073915

Regions

Topological domain1 – 8585Lumenal Potential
Transmembrane86 – 10621Helical; Potential
Topological domain107 – 15650Cytoplasmic Potential
Transmembrane157 – 17721Helical; Potential
Topological domain178 – 19215Lumenal Potential
Transmembrane193 – 21321Helical; Potential
Topological domain214 – 30390Cytoplasmic Potential
Transmembrane304 – 32421Helical; Potential
Topological domain325 – 3284Lumenal Potential
Transmembrane329 – 34921Helical; Potential
Topological domain350 – 3512Cytoplasmic Potential
Transmembrane352 – 37221Helical; Potential
Topological domain373 – 40129Lumenal Potential
Transmembrane402 – 42221Helical; Potential
Topological domain423 – 45028Cytoplasmic Potential
Transmembrane451 – 47121Helical; Potential
Topological domain472 – 4754Lumenal Potential
Transmembrane476 – 49621Helical; Potential
Topological domain497 – 58791Cytoplasmic Potential
Motif477 – 4793PAL
Compositional bias550 – 58738Asp/Glu-rich (highly acidic)

Sites

Active site3661 By similarity
Active site4111 By similarity

Sequences

Sequence LengthMass (Da)Tools
P34248 [UniParc].

Last modified February 1, 1994. Version 1.
Checksum: 40611E339C6FD388

FASTA58767,525
        10         20         30         40         50         60 
MDKYLNSFVD HLSEWSSRAF RNNSSSANQS ASNKELEQVF EQINAIVENH NNKLTTAFDK 

        70         80         90        100        110        120 
ISYRVAHKIT HLVESHSLVF NYATLVLIAS ALVVIGSFTS ISSIPFTALP PTREHSLFDP 

       130        140        150        160        170        180 
TDFDVDHDCH VIYRENDEDK KKKKKSKRFF DMMDEKHAII LPLTSGCTLL ALYFVIKKLH 

       190        200        210        220        230        240 
LNWLKYVVKI LNFNITLLNI PAGTFVYSYF LNSLFRNLSH LASWNPLVVL PRYRVTIADD 

       250        260        270        280        290        300 
NEDLNKIGGF VTNLNYKDGL TNSVVHKKTL DEIEKDHWMK HFYRRELVEP KDIKSKRQIS 

       310        320        330        340        350        360 
NMYLNSALIV SFVLSIVSTV YFYLSPNDWL ISNAVSMNMA IWSIAQLKLK NLKSGALILI 

       370        380        390        400        410        420 
ALFFYDICFV FGTDVMVTVA TNLDIPVKLS LPVKFNTAQN NFNFSILGLG DIALPGMFIA 

       430        440        450        460        470        480 
MCYKYDIWKW HLDHDDTEFH FLNWSYVGKY FITAMVSYVA SLVSAMVSLS IFNTAQPALL 

       490        500        510        520        530        540 
YIVPSLLIST ILVACWNKDF KQFWNFQYDT IEVDKSLKKA IEKKENSITY STFILSEYYN 

       550        560        570        580 
DADKYALLGD DVNENFDDDE EFVQEEDLSD SSEEELSEED LLDDESS

« Hide

References

« Hide 'large scale' references
[1]"DNA sequence analysis of a 17 kb fragment of yeast chromosome XI physically localizes the MRB1 gene and reveals eight new open reading frames, including a homologue of the KIN1/KIN2 and SNF1 protein kinases."
Pallier C., Valens M., Puzos V., Fukuhara H., Cheret G., Sor F., Bolotin-Fukuhara M.
Yeast 9:1149-1155(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[2]"Complete DNA sequence of yeast chromosome XI."
Dujon B., Alexandraki D., Andre B., Ansorge W., Baladron V., Ballesta J.P.G., Banrevi A., Bolle P.-A., Bolotin-Fukuhara M., Bossier P., Bou G., Boyer J., Buitrago M.J., Cheret G., Colleaux L., Daignan-Fornier B., del Rey F., Dion C. expand/collapse author list , Domdey H., Duesterhoeft A., Duesterhus S., Entian K.-D., Erfle H., Esteban P.F., Feldmann H., Fernandes L., Fobo G.M., Fritz C., Fukuhara H., Gabel C., Gaillon L., Garcia-Cantalejo J.M., Garcia-Ramirez J.J., Gent M.E., Ghazvini M., Goffeau A., Gonzalez A., Grothues D., Guerreiro P., Hegemann J.H., Hewitt N., Hilger F., Hollenberg C.P., Horaitis O., Indge K.J., Jacquier A., James C.M., Jauniaux J.-C., Jimenez A., Keuchel H., Kirchrath L., Kleine K., Koetter P., Legrain P., Liebl S., Louis E.J., Maia e Silva A., Marck C., Monnier A.-L., Moestl D., Mueller S., Obermaier B., Oliver S.G., Pallier C., Pascolo S., Pfeiffer F., Philippsen P., Planta R.J., Pohl F.M., Pohl T.M., Poehlmann R., Portetelle D., Purnelle B., Puzos V., Ramezani Rad M., Rasmussen S.W., Remacha M.A., Revuelta J.L., Richard G.-F., Rieger M., Rodrigues-Pousada C., Rose M., Rupp T., Santos M.A., Schwager C., Sensen C., Skala J., Soares H., Sor F., Stegemann J., Tettelin H., Thierry A., Tzermia M., Urrestarazu L.A., van Dyck L., van Vliet-Reedijk J.C., Valens M., Vandenbol M., Vilela C., Vissers S., von Wettstein D., Voss H., Wiemann S., Xu G., Zimmermann J., Haasemann M., Becker I., Mewes H.-W.
Nature 369:371-378(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 96604 / S288c / FY1679.
[3]Saccharomyces Genome Database
Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[4]"A global topology map of the Saccharomyces cerevisiae membrane proteome."
Kim H., Melen K., Oesterberg M., von Heijne G.
Proc. Natl. Acad. Sci. U.S.A. 103:11142-11147(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: TOPOLOGY [LARGE SCALE ANALYSIS].
Strain: ATCC 208353 / W303-1A.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		X71133 Genomic DNA. Translation: CAA50457.1.
Z28100 Genomic DNA. Translation: CAA81940.1.
BK006944 Genomic DNA. Translation: DAA09057.1.
PIRS37927.
RefSeqNP_012822.1. NM_001179666.1.

3D structure databases

ProteinModelPortalP34248.
ModBaseSearch...

Protein-protein interaction databases

IntActP34248. 12 interactions.
MINTMINT-2491796.
STRING4932.YKL100C.

Protein family/group databases

MEROPSA22.008.

Proteomic databases

PaxDbP34248.
PeptideAtlasP34248.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYKL100C; YKL100C; YKL100C.
GeneID853761.
KEGGsce:YKL100C.

Organism-specific databases

CYGDYKL100c.
SGDS000001583. YKL100C.

Phylogenomic databases

eggNOGNOG313636.
GeneTreeENSGT00530000062920.
HOGENOMHOG000001080.
OMAFIAMCYK.
OrthoDBEOG4VHPFV.

Gene expression databases

GenevestigatorP34248.
GermOnlineYKL100C. Saccharomyces cerevisiae.

Family and domain databases

InterProIPR006639. Peptidase_A22.
IPR007369. Peptidase_A22B_SPP.
[Graphical view]
PANTHERPTHR12174. PTHR12174. 1 hit.
PfamPF04258. Peptidase_A22B. 1 hit.
[Graphical view]
SMARTSM00730. PSN. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio974843.

Entry information

Entry nameYKK0_YEAST
AccessionPrimary (citable) accession number: P34248
Secondary accession number(s): D6VXI7
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: February 1, 1994
Last modified: April 3, 2013
This is version 92 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

Yeast chromosome XI

Yeast (Saccharomyces cerevisiae) chromosome XI: entries and gene names

Peptidase families

Classification of peptidase families and list of entries

SIMILARITY comments

Index of protein domains and families

to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents