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P34243

- HCS1_YEAST

UniProt

P34243 - HCS1_YEAST

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Protein

DNA polymerase alpha-associated DNA helicase A

Gene

HCS1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

DNA polymerase alpha-associated DNA helicase which may be involved in DNA replication.5 Publications

Catalytic activityi

ATP + H2O = ADP + phosphate.

Kineticsi

  1. KM=90 µM for ATP2 Publications

Vmax=14 µmol/min/mg enzyme2 Publications

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi229 – 2368ATPSequence Analysis

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. ATP-dependent 5'-3' DNA helicase activity Source: SGD
  3. DNA binding Source: InterPro

GO - Biological processi

  1. DNA duplex unwinding Source: GOC
  2. lagging strand elongation Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Helicase, Hydrolase

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-31826-MONOMER.
SABIO-RKP34243.

Names & Taxonomyi

Protein namesi
Recommended name:
DNA polymerase alpha-associated DNA helicase A (EC:3.6.4.12)
Gene namesi
Name:HCS1
Synonyms:DIP1
Ordered Locus Names:YKL017C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome XI

Organism-specific databases

CYGDiYKL017c.
SGDiS000001500. HCS1.

Subcellular locationi

Cytoplasm 1 Publication. Nucleus 1 Publication

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-KW
  2. DNA helicase A complex Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 683683DNA polymerase alpha-associated DNA helicase APRO_0000080726Add
BLAST

Proteomic databases

MaxQBiP34243.
PaxDbiP34243.

Expressioni

Inductioni

By heat shock.1 Publication

Gene expression databases

GenevestigatoriP34243.

Interactioni

Subunit structurei

Associates with the hexameric DNA polymerase alpha.

Protein-protein interaction databases

BioGridi34115. 11 interactions.
DIPiDIP-1383N.
IntActiP34243. 1 interaction.
MINTiMINT-399891.
STRINGi4932.YKL017C.

Structurei

3D structure databases

ProteinModelPortaliP34243.
SMRiP34243. Positions 117-655.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the DNA2/NAM7 helicase family.Curated

Phylogenomic databases

eggNOGiCOG1112.
GeneTreeiENSGT00730000111172.
HOGENOMiHOG000185831.
InParanoidiP34243.
OMAiFQGRENE.
OrthoDBiEOG70PC5T.

Family and domain databases

Gene3Di3.40.50.300. 3 hits.
InterProiIPR003593. AAA+_ATPase.
IPR014001. Helicase_ATP-bd.
IPR027417. P-loop_NTPase.
IPR004483. SMUBP-2/Hcs1_like.
[Graphical view]
SMARTiSM00382. AAA. 1 hit.
SM00487. DEXDc. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00376. TIGR00376. 1 hit.

Sequencei

Sequence statusi: Complete.

P34243-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MNKELASKFL SSIKHEREQD IQTTSRLLTT LSIQQLVQNG LAINNIHLEN
60 70 80 90 100
IRSGLIGKLY MELGPNLAVN DKIQRGDIKV GDIVLVRPAK TKVNTKTKPK
110 120 130 140 150
VKKVSEDSNG EQAECSGVVY KMSDTQITIA LEESQDVIAT TFYSYSKLYI
160 170 180 190 200
LKTTNVVTYN RMESTMRKLS EISSPIQDKI IQYLVNERPF IPNTNSFQNI
210 220 230 240 250
KSFLNPNLND SQKTAINFAI NNDLTIIHGP PGTGKTFTLI ELIQQLLIKN
260 270 280 290 300
PEERILICGP SNISVDTILE RLTPLVPNNL LLRIGHPARL LDSNKRHSLD
310 320 330 340 350
ILSKKNTIVK DISQEIDKLI QENKKLKNYK QRKENWNEIK LLRKDLKKRE
360 370 380 390 400
FKTIKDLIIQ SRIVVTTLHG SSSRELCSLY RDDPNFQLFD TLIIDEVSQA
410 420 430 440 450
MEPQCWIPLI AHQNQFHKLV LAGDNKQLPP TIKTEDDKNV IHNLETTLFD
460 470 480 490 500
RIIKIFPKRD MVKFLNVQYR MNQKIMEFPS HSMYNGKLLA DATVANRLLI
510 520 530 540 550
DLPTVDATPS EDDDDTKIPL IWYDTQGDEF QETADEATIL GSKYNEGEIA
560 570 580 590 600
IVKEHIENLR SFNVPENSIG VISPYNAQVS HLKKLIHDEL KLTDIEISTV
610 620 630 640 650
DGFQGREKDV IILSLVRSNE KFEVGFLKEE RRLNVAMTRP RRQLVVVGNI
660 670 680
EVLQRCGNKY LKSWSEWCEE NADVRYPNID DYL
Length:683
Mass (Da):78,273
Last modified:February 1, 1994 - v1
Checksum:iFCD02680757377B8
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X74152 Genomic DNA. Translation: CAA52266.1.
Z28017 Genomic DNA. Translation: CAA81852.1.
BK006944 Genomic DNA. Translation: DAA09139.1.
PIRiS34700.
RefSeqiNP_012908.1. NM_001179583.1.

Genome annotation databases

EnsemblFungiiYKL017C; YKL017C; YKL017C.
GeneIDi853852.
KEGGisce:YKL017C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X74152 Genomic DNA. Translation: CAA52266.1 .
Z28017 Genomic DNA. Translation: CAA81852.1 .
BK006944 Genomic DNA. Translation: DAA09139.1 .
PIRi S34700.
RefSeqi NP_012908.1. NM_001179583.1.

3D structure databases

ProteinModelPortali P34243.
SMRi P34243. Positions 117-655.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 34115. 11 interactions.
DIPi DIP-1383N.
IntActi P34243. 1 interaction.
MINTi MINT-399891.
STRINGi 4932.YKL017C.

Proteomic databases

MaxQBi P34243.
PaxDbi P34243.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YKL017C ; YKL017C ; YKL017C .
GeneIDi 853852.
KEGGi sce:YKL017C.

Organism-specific databases

CYGDi YKL017c.
SGDi S000001500. HCS1.

Phylogenomic databases

eggNOGi COG1112.
GeneTreei ENSGT00730000111172.
HOGENOMi HOG000185831.
InParanoidi P34243.
OMAi FQGRENE.
OrthoDBi EOG70PC5T.

Enzyme and pathway databases

BioCyci YEAST:G3O-31826-MONOMER.
SABIO-RK P34243.

Miscellaneous databases

NextBioi 975085.
PROi P34243.

Gene expression databases

Genevestigatori P34243.

Family and domain databases

Gene3Di 3.40.50.300. 3 hits.
InterProi IPR003593. AAA+_ATPase.
IPR014001. Helicase_ATP-bd.
IPR027417. P-loop_NTPase.
IPR004483. SMUBP-2/Hcs1_like.
[Graphical view ]
SMARTi SM00382. AAA. 1 hit.
SM00487. DEXDc. 1 hit.
[Graphical view ]
SUPFAMi SSF52540. SSF52540. 1 hit.
TIGRFAMsi TIGR00376. TIGR00376. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Sequencing and analysis of 51.6 kilobases on the left arm of chromosome XI from Saccharomyces cerevisiae reveals 23 open reading frames including the FAS1 gene."
    Wiemann S., Voss H., Schwager C., Rupp T., Stegemann J., Zimmermann J., Grothues D., Sensen C., Erfle H., Hewitt N., Banrevi A., Ansorge W.
    Yeast 9:1343-1348(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "Complete DNA sequence of yeast chromosome XI."
    Dujon B., Alexandraki D., Andre B., Ansorge W., Baladron V., Ballesta J.P.G., Banrevi A., Bolle P.-A., Bolotin-Fukuhara M., Bossier P., Bou G., Boyer J., Buitrago M.J., Cheret G., Colleaux L., Daignan-Fornier B., del Rey F., Dion C.
    , Domdey H., Duesterhoeft A., Duesterhus S., Entian K.-D., Erfle H., Esteban P.F., Feldmann H., Fernandes L., Fobo G.M., Fritz C., Fukuhara H., Gabel C., Gaillon L., Garcia-Cantalejo J.M., Garcia-Ramirez J.J., Gent M.E., Ghazvini M., Goffeau A., Gonzalez A., Grothues D., Guerreiro P., Hegemann J.H., Hewitt N., Hilger F., Hollenberg C.P., Horaitis O., Indge K.J., Jacquier A., James C.M., Jauniaux J.-C., Jimenez A., Keuchel H., Kirchrath L., Kleine K., Koetter P., Legrain P., Liebl S., Louis E.J., Maia e Silva A., Marck C., Monnier A.-L., Moestl D., Mueller S., Obermaier B., Oliver S.G., Pallier C., Pascolo S., Pfeiffer F., Philippsen P., Planta R.J., Pohl F.M., Pohl T.M., Poehlmann R., Portetelle D., Purnelle B., Puzos V., Ramezani Rad M., Rasmussen S.W., Remacha M.A., Revuelta J.L., Richard G.-F., Rieger M., Rodrigues-Pousada C., Rose M., Rupp T., Santos M.A., Schwager C., Sensen C., Skala J., Soares H., Sor F., Stegemann J., Tettelin H., Thierry A., Tzermia M., Urrestarazu L.A., van Dyck L., van Vliet-Reedijk J.C., Valens M., Vandenbol M., Vilela C., Vissers S., von Wettstein D., Voss H., Wiemann S., Xu G., Zimmermann J., Haasemann M., Becker I., Mewes H.-W.
    Nature 369:371-378(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  4. "Purification and characterization of DNA polymerase alpha-associated replication protein A-dependent yeast DNA helicase A."
    Biswas S.B., Chen P.H., Biswas E.E.
    Biochemistry 36:13270-13276(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 9-22, FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES.
  5. "Purification and characterization of a yeast DNA polymerase alpha complex with associated primase, 5'-->3' exonuclease, and DNA-dependent ATPase activities."
    Biswas E.E., Chen P.H., Gray W., Li Y.H., Ray S., Biswas S.B.
    Biochemistry 32:3013-3019(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, ASSOCIATION WITH DNA POLYMERASE ALPHA.
  6. "Characterization of the DNA-dependent ATPase and a DNA unwinding activity associated with the yeast DNA polymerase alpha complex."
    Biswas E.E., Ewing C.M., Biswas S.B.
    Biochemistry 32:3020-3026(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, ASSOCIATION WITH DNA POLYMERASE ALPHA.
  7. "DNA helicase associated with DNA polymerase alpha: isolation by a modified immunoaffinity chromatography."
    Biswas E.E., Chen P.H., Biswas S.B.
    Biochemistry 32:13393-13398(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, ASSOCIATION WITH DNA POLYMERASE ALPHA.
  8. "Yeast DNA helicase A: cloning, expression, purification, and enzymatic characterization."
    Biswas E.E., Fricke W.M., Chen P.H., Biswas S.B.
    Biochemistry 36:13277-13284(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES.
  9. "Systematic identification, classification, and characterization of the open reading frames which encode novel helicase-related proteins in Saccharomyces cerevisiae by gene disruption and Northern analysis."
    Shiratori A., Shibata T., Arisawa M., Hanaoka F., Murakami Y., Eki T.
    Yeast 15:219-253(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: INDUCTION.
  10. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
  11. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiHCS1_YEAST
AccessioniPrimary (citable) accession number: P34243
Secondary accession number(s): D6VXR9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: February 1, 1994
Last modified: October 29, 2014
This is version 113 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 1040 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XI
    Yeast (Saccharomyces cerevisiae) chromosome XI: entries and gene names

External Data

Dasty 3