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P34243

- HCS1_YEAST

UniProt

P34243 - HCS1_YEAST

Protein

DNA polymerase alpha-associated DNA helicase A

Gene

HCS1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 112 (01 Oct 2014)
      Sequence version 1 (01 Feb 1994)
      Previous versions | rss
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    Functioni

    DNA polymerase alpha-associated DNA helicase which may be involved in DNA replication.5 Publications

    Catalytic activityi

    ATP + H2O = ADP + phosphate.

    Kineticsi

    1. KM=90 µM for ATP2 Publications

    Vmax=14 µmol/min/mg enzyme2 Publications

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi229 – 2368ATPSequence Analysis

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. ATP-dependent 5'-3' DNA helicase activity Source: SGD
    3. DNA binding Source: InterPro

    GO - Biological processi

    1. DNA duplex unwinding Source: GOC
    2. lagging strand elongation Source: SGD

    Keywords - Molecular functioni

    Helicase, Hydrolase

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciYEAST:G3O-31826-MONOMER.
    SABIO-RKP34243.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    DNA polymerase alpha-associated DNA helicase A (EC:3.6.4.12)
    Gene namesi
    Name:HCS1
    Synonyms:DIP1
    Ordered Locus Names:YKL017C
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome XI

    Organism-specific databases

    CYGDiYKL017c.
    SGDiS000001500. HCS1.

    Subcellular locationi

    Cytoplasm 1 Publication. Nucleus 1 Publication

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell
    2. DNA helicase A complex Source: SGD

    Keywords - Cellular componenti

    Cytoplasm, Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 683683DNA polymerase alpha-associated DNA helicase APRO_0000080726Add
    BLAST

    Proteomic databases

    MaxQBiP34243.
    PaxDbiP34243.

    Expressioni

    Inductioni

    By heat shock.1 Publication

    Gene expression databases

    GenevestigatoriP34243.

    Interactioni

    Subunit structurei

    Associates with the hexameric DNA polymerase alpha.

    Protein-protein interaction databases

    BioGridi34115. 9 interactions.
    DIPiDIP-1383N.
    IntActiP34243. 1 interaction.
    MINTiMINT-399891.
    STRINGi4932.YKL017C.

    Structurei

    3D structure databases

    ProteinModelPortaliP34243.
    SMRiP34243. Positions 117-655.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the DNA2/NAM7 helicase family.Curated

    Phylogenomic databases

    eggNOGiCOG1112.
    GeneTreeiENSGT00730000111172.
    HOGENOMiHOG000185831.
    OMAiFQGRENE.
    OrthoDBiEOG70PC5T.

    Family and domain databases

    Gene3Di3.40.50.300. 3 hits.
    InterProiIPR003593. AAA+_ATPase.
    IPR014001. Helicase_ATP-bd.
    IPR027417. P-loop_NTPase.
    IPR004483. SMUBP-2/Hcs1_like.
    [Graphical view]
    SMARTiSM00382. AAA. 1 hit.
    SM00487. DEXDc. 1 hit.
    [Graphical view]
    SUPFAMiSSF52540. SSF52540. 1 hit.
    TIGRFAMsiTIGR00376. TIGR00376. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    P34243-1 [UniParc]FASTAAdd to Basket

    « Hide

    MNKELASKFL SSIKHEREQD IQTTSRLLTT LSIQQLVQNG LAINNIHLEN    50
    IRSGLIGKLY MELGPNLAVN DKIQRGDIKV GDIVLVRPAK TKVNTKTKPK 100
    VKKVSEDSNG EQAECSGVVY KMSDTQITIA LEESQDVIAT TFYSYSKLYI 150
    LKTTNVVTYN RMESTMRKLS EISSPIQDKI IQYLVNERPF IPNTNSFQNI 200
    KSFLNPNLND SQKTAINFAI NNDLTIIHGP PGTGKTFTLI ELIQQLLIKN 250
    PEERILICGP SNISVDTILE RLTPLVPNNL LLRIGHPARL LDSNKRHSLD 300
    ILSKKNTIVK DISQEIDKLI QENKKLKNYK QRKENWNEIK LLRKDLKKRE 350
    FKTIKDLIIQ SRIVVTTLHG SSSRELCSLY RDDPNFQLFD TLIIDEVSQA 400
    MEPQCWIPLI AHQNQFHKLV LAGDNKQLPP TIKTEDDKNV IHNLETTLFD 450
    RIIKIFPKRD MVKFLNVQYR MNQKIMEFPS HSMYNGKLLA DATVANRLLI 500
    DLPTVDATPS EDDDDTKIPL IWYDTQGDEF QETADEATIL GSKYNEGEIA 550
    IVKEHIENLR SFNVPENSIG VISPYNAQVS HLKKLIHDEL KLTDIEISTV 600
    DGFQGREKDV IILSLVRSNE KFEVGFLKEE RRLNVAMTRP RRQLVVVGNI 650
    EVLQRCGNKY LKSWSEWCEE NADVRYPNID DYL 683
    Length:683
    Mass (Da):78,273
    Last modified:February 1, 1994 - v1
    Checksum:iFCD02680757377B8
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X74152 Genomic DNA. Translation: CAA52266.1.
    Z28017 Genomic DNA. Translation: CAA81852.1.
    BK006944 Genomic DNA. Translation: DAA09139.1.
    PIRiS34700.
    RefSeqiNP_012908.1. NM_001179583.1.

    Genome annotation databases

    EnsemblFungiiYKL017C; YKL017C; YKL017C.
    GeneIDi853852.
    KEGGisce:YKL017C.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X74152 Genomic DNA. Translation: CAA52266.1 .
    Z28017 Genomic DNA. Translation: CAA81852.1 .
    BK006944 Genomic DNA. Translation: DAA09139.1 .
    PIRi S34700.
    RefSeqi NP_012908.1. NM_001179583.1.

    3D structure databases

    ProteinModelPortali P34243.
    SMRi P34243. Positions 117-655.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 34115. 9 interactions.
    DIPi DIP-1383N.
    IntActi P34243. 1 interaction.
    MINTi MINT-399891.
    STRINGi 4932.YKL017C.

    Proteomic databases

    MaxQBi P34243.
    PaxDbi P34243.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YKL017C ; YKL017C ; YKL017C .
    GeneIDi 853852.
    KEGGi sce:YKL017C.

    Organism-specific databases

    CYGDi YKL017c.
    SGDi S000001500. HCS1.

    Phylogenomic databases

    eggNOGi COG1112.
    GeneTreei ENSGT00730000111172.
    HOGENOMi HOG000185831.
    OMAi FQGRENE.
    OrthoDBi EOG70PC5T.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-31826-MONOMER.
    SABIO-RK P34243.

    Miscellaneous databases

    NextBioi 975085.
    PROi P34243.

    Gene expression databases

    Genevestigatori P34243.

    Family and domain databases

    Gene3Di 3.40.50.300. 3 hits.
    InterProi IPR003593. AAA+_ATPase.
    IPR014001. Helicase_ATP-bd.
    IPR027417. P-loop_NTPase.
    IPR004483. SMUBP-2/Hcs1_like.
    [Graphical view ]
    SMARTi SM00382. AAA. 1 hit.
    SM00487. DEXDc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF52540. SSF52540. 1 hit.
    TIGRFAMsi TIGR00376. TIGR00376. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Sequencing and analysis of 51.6 kilobases on the left arm of chromosome XI from Saccharomyces cerevisiae reveals 23 open reading frames including the FAS1 gene."
      Wiemann S., Voss H., Schwager C., Rupp T., Stegemann J., Zimmermann J., Grothues D., Sensen C., Erfle H., Hewitt N., Banrevi A., Ansorge W.
      Yeast 9:1343-1348(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. "Complete DNA sequence of yeast chromosome XI."
      Dujon B., Alexandraki D., Andre B., Ansorge W., Baladron V., Ballesta J.P.G., Banrevi A., Bolle P.-A., Bolotin-Fukuhara M., Bossier P., Bou G., Boyer J., Buitrago M.J., Cheret G., Colleaux L., Daignan-Fornier B., del Rey F., Dion C.
      , Domdey H., Duesterhoeft A., Duesterhus S., Entian K.-D., Erfle H., Esteban P.F., Feldmann H., Fernandes L., Fobo G.M., Fritz C., Fukuhara H., Gabel C., Gaillon L., Garcia-Cantalejo J.M., Garcia-Ramirez J.J., Gent M.E., Ghazvini M., Goffeau A., Gonzalez A., Grothues D., Guerreiro P., Hegemann J.H., Hewitt N., Hilger F., Hollenberg C.P., Horaitis O., Indge K.J., Jacquier A., James C.M., Jauniaux J.-C., Jimenez A., Keuchel H., Kirchrath L., Kleine K., Koetter P., Legrain P., Liebl S., Louis E.J., Maia e Silva A., Marck C., Monnier A.-L., Moestl D., Mueller S., Obermaier B., Oliver S.G., Pallier C., Pascolo S., Pfeiffer F., Philippsen P., Planta R.J., Pohl F.M., Pohl T.M., Poehlmann R., Portetelle D., Purnelle B., Puzos V., Ramezani Rad M., Rasmussen S.W., Remacha M.A., Revuelta J.L., Richard G.-F., Rieger M., Rodrigues-Pousada C., Rose M., Rupp T., Santos M.A., Schwager C., Sensen C., Skala J., Soares H., Sor F., Stegemann J., Tettelin H., Thierry A., Tzermia M., Urrestarazu L.A., van Dyck L., van Vliet-Reedijk J.C., Valens M., Vandenbol M., Vilela C., Vissers S., von Wettstein D., Voss H., Wiemann S., Xu G., Zimmermann J., Haasemann M., Becker I., Mewes H.-W.
      Nature 369:371-378(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    3. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    4. "Purification and characterization of DNA polymerase alpha-associated replication protein A-dependent yeast DNA helicase A."
      Biswas S.B., Chen P.H., Biswas E.E.
      Biochemistry 36:13270-13276(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 9-22, FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES.
    5. "Purification and characterization of a yeast DNA polymerase alpha complex with associated primase, 5'-->3' exonuclease, and DNA-dependent ATPase activities."
      Biswas E.E., Chen P.H., Gray W., Li Y.H., Ray S., Biswas S.B.
      Biochemistry 32:3013-3019(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, ASSOCIATION WITH DNA POLYMERASE ALPHA.
    6. "Characterization of the DNA-dependent ATPase and a DNA unwinding activity associated with the yeast DNA polymerase alpha complex."
      Biswas E.E., Ewing C.M., Biswas S.B.
      Biochemistry 32:3020-3026(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, ASSOCIATION WITH DNA POLYMERASE ALPHA.
    7. "DNA helicase associated with DNA polymerase alpha: isolation by a modified immunoaffinity chromatography."
      Biswas E.E., Chen P.H., Biswas S.B.
      Biochemistry 32:13393-13398(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, ASSOCIATION WITH DNA POLYMERASE ALPHA.
    8. "Yeast DNA helicase A: cloning, expression, purification, and enzymatic characterization."
      Biswas E.E., Fricke W.M., Chen P.H., Biswas S.B.
      Biochemistry 36:13277-13284(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES.
    9. "Systematic identification, classification, and characterization of the open reading frames which encode novel helicase-related proteins in Saccharomyces cerevisiae by gene disruption and Northern analysis."
      Shiratori A., Shibata T., Arisawa M., Hanaoka F., Murakami Y., Eki T.
      Yeast 15:219-253(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: INDUCTION.
    10. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
    11. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiHCS1_YEAST
    AccessioniPrimary (citable) accession number: P34243
    Secondary accession number(s): D6VXR9
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1994
    Last sequence update: February 1, 1994
    Last modified: October 1, 2014
    This is version 112 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 1040 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    3. Yeast chromosome XI
      Yeast (Saccharomyces cerevisiae) chromosome XI: entries and gene names

    External Data

    Dasty 3