P34243 (HCS1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
DNA polymerase alpha-associated DNA helicase A
EC=3.6.4.12

Gene names

Name:	HCS1
Synonyms:	DIP1
Ordered Locus Names:	YKL017C

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › Saccharomyces cerevisiae

Protein attributes

Sequence length	683 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	DNA polymerase alpha-associated DNA helicase which may be involved in DNA replication. Ref.4 Ref.5 Ref.6 Ref.7 Ref.8
Catalytic activity	ATP + H2O = ADP + phosphate.
Subunit structure	Associates with the hexameric DNA polymerase alpha.
Subcellular location	Cytoplasm. Nucleus Probable Ref.10.
Induction	By heat shock. Ref.9
Miscellaneous	Present with 1040 molecules/cell in log phase SD medium.
Sequence similarities	Belongs to the DNA2/NAM7 helicase family.
Biophysicochemical properties	Kinetic parameters: KM=90 µM for ATP Ref.4 Ref.8 Vmax=14 µmol/min/mg enzyme

Ontologies

Keywords
Cellular component	Cytoplasm Nucleus
Ligand	ATP-binding Nucleotide-binding
Molecular function	Helicase Hydrolase
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	lagging strand elongation Inferred by curator Ref.7. Source: SGD
Cellular_component	DNA helicase A complex Inferred from direct assay Ref.8. Source: SGD cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW ATP-dependent 5'-3' DNA helicase activity Inferred from direct assay Ref.7Ref.8. Source: SGD DNA binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 683	683	DNA polymerase alpha-associated DNA helicase A		PRO_0000080726
Regions
	Nucleotide binding	229 – 236	8	ATP Potential

Sequences

	Sequence		Length	Mass (Da)
	P34243 [UniParc]. Last modified February 1, 1994. Version 1. Checksum: FCD02680757377B8	FASTA	683	78,273
	10 20 30 40 50 60 MNKELASKFL SSIKHEREQD IQTTSRLLTT LSIQQLVQNG LAINNIHLEN IRSGLIGKLY 70 80 90 100 110 120 MELGPNLAVN DKIQRGDIKV GDIVLVRPAK TKVNTKTKPK VKKVSEDSNG EQAECSGVVY 130 140 150 160 170 180 KMSDTQITIA LEESQDVIAT TFYSYSKLYI LKTTNVVTYN RMESTMRKLS EISSPIQDKI 190 200 210 220 230 240 IQYLVNERPF IPNTNSFQNI KSFLNPNLND SQKTAINFAI NNDLTIIHGP PGTGKTFTLI 250 260 270 280 290 300 ELIQQLLIKN PEERILICGP SNISVDTILE RLTPLVPNNL LLRIGHPARL LDSNKRHSLD 310 320 330 340 350 360 ILSKKNTIVK DISQEIDKLI QENKKLKNYK QRKENWNEIK LLRKDLKKRE FKTIKDLIIQ 370 380 390 400 410 420 SRIVVTTLHG SSSRELCSLY RDDPNFQLFD TLIIDEVSQA MEPQCWIPLI AHQNQFHKLV 430 440 450 460 470 480 LAGDNKQLPP TIKTEDDKNV IHNLETTLFD RIIKIFPKRD MVKFLNVQYR MNQKIMEFPS 490 500 510 520 530 540 HSMYNGKLLA DATVANRLLI DLPTVDATPS EDDDDTKIPL IWYDTQGDEF QETADEATIL 550 560 570 580 590 600 GSKYNEGEIA IVKEHIENLR SFNVPENSIG VISPYNAQVS HLKKLIHDEL KLTDIEISTV 610 620 630 640 650 660 DGFQGREKDV IILSLVRSNE KFEVGFLKEE RRLNVAMTRP RRQLVVVGNI EVLQRCGNKY 670 680 LKSWSEWCEE NADVRYPNID DYL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequencing and analysis of 51.6 kilobases on the left arm of chromosome XI from Saccharomyces cerevisiae reveals 23 open reading frames including the FAS1 gene." Wiemann S., Voss H., Schwager C., Rupp T., Stegemann J., Zimmermann J., Grothues D., Sensen C., Erfle H., Hewitt N., Banrevi A., Ansorge W. Yeast 9:1343-1348(1993) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Complete DNA sequence of yeast chromosome XI." Dujon B., Alexandraki D., Andre B., Ansorge W., Baladron V., Ballesta J.P.G., Banrevi A., Bolle P.-A., Bolotin-Fukuhara M., Bossier P., Bou G., Boyer J., Buitrago M.J., Cheret G., Colleaux L., Daignan-Fornier B., del Rey F., Dion C. , Domdey H., Duesterhoeft A., Duesterhus S., Entian K.-D., Erfle H., Esteban P.F., Feldmann H., Fernandes L., Fobo G.M., Fritz C., Fukuhara H., Gabel C., Gaillon L., Garcia-Cantalejo J.M., Garcia-Ramirez J.J., Gent M.E., Ghazvini M., Goffeau A., Gonzalez A., Grothues D., Guerreiro P., Hegemann J.H., Hewitt N., Hilger F., Hollenberg C.P., Horaitis O., Indge K.J., Jacquier A., James C.M., Jauniaux J.-C., Jimenez A., Keuchel H., Kirchrath L., Kleine K., Koetter P., Legrain P., Liebl S., Louis E.J., Maia e Silva A., Marck C., Monnier A.-L., Moestl D., Mueller S., Obermaier B., Oliver S.G., Pallier C., Pascolo S., Pfeiffer F., Philippsen P., Planta R.J., Pohl F.M., Pohl T.M., Poehlmann R., Portetelle D., Purnelle B., Puzos V., Ramezani Rad M., Rasmussen S.W., Remacha M.A., Revuelta J.L., Richard G.-F., Rieger M., Rodrigues-Pousada C., Rose M., Rupp T., Santos M.A., Schwager C., Sensen C., Skala J., Soares H., Sor F., Stegemann J., Tettelin H., Thierry A., Tzermia M., Urrestarazu L.A., van Dyck L., van Vliet-Reedijk J.C., Valens M., Vandenbol M., Vilela C., Vissers S., von Wettstein D., Voss H., Wiemann S., Xu G., Zimmermann J., Haasemann M., Becker I., Mewes H.-W. Nature 369:371-378(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[3]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[4]	"Purification and characterization of DNA polymerase alpha-associated replication protein A-dependent yeast DNA helicase A." Biswas S.B., Chen P.H., Biswas E.E. Biochemistry 36:13270-13276(1997) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 9-22, FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES.
[5]	"Purification and characterization of a yeast DNA polymerase alpha complex with associated primase, 5'-->3' exonuclease, and DNA-dependent ATPase activities." Biswas E.E., Chen P.H., Gray W., Li Y.H., Ray S., Biswas S.B. Biochemistry 32:3013-3019(1993) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, ASSOCIATION WITH DNA POLYMERASE ALPHA.
[6]	"Characterization of the DNA-dependent ATPase and a DNA unwinding activity associated with the yeast DNA polymerase alpha complex." Biswas E.E., Ewing C.M., Biswas S.B. Biochemistry 32:3020-3026(1993) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, ASSOCIATION WITH DNA POLYMERASE ALPHA.
[7]	"DNA helicase associated with DNA polymerase alpha: isolation by a modified immunoaffinity chromatography." Biswas E.E., Chen P.H., Biswas S.B. Biochemistry 32:13393-13398(1993) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, ASSOCIATION WITH DNA POLYMERASE ALPHA.
[8]	"Yeast DNA helicase A: cloning, expression, purification, and enzymatic characterization." Biswas E.E., Fricke W.M., Chen P.H., Biswas S.B. Biochemistry 36:13277-13284(1997) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES.
[9]	"Systematic identification, classification, and characterization of the open reading frames which encode novel helicase-related proteins in Saccharomyces cerevisiae by gene disruption and Northern analysis." Shiratori A., Shibata T., Arisawa M., Hanaoka F., Murakami Y., Eki T. Yeast 15:219-253(1999) [PubMed] [Europe PMC] [Abstract] Cited for: INDUCTION.
[10]	"Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[11]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X74152 Genomic DNA. Translation: CAA52266.1. Z28017 Genomic DNA. Translation: CAA81852.1. BK006944 Genomic DNA. Translation: DAA09139.1.
PIR	S34700.
RefSeq	NP_012908.1. NM_001179583.1.
3D structure databases
ProteinModelPortal	P34243.
SMR	P34243. Positions 117-655.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-1383N.
IntAct	P34243. 1 interaction.
MINT	MINT-399891.
STRING	4932.YKL017C.
Proteomic databases
PaxDb	P34243.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YKL017C; YKL017C; YKL017C.
GeneID	853852.
KEGG	sce:YKL017C.
Organism-specific databases
CYGD	YKL017c.
SGD	S000001500. HCS1.
Phylogenomic databases
eggNOG	COG1112.
GeneTree	ENSGT00700000104572.
HOGENOM	HOG000185831.
OMA	QGGDFPE.
OrthoDB	EOG444PTN.
Enzyme and pathway databases
SABIO-RK	P34243.
Gene expression databases
Genevestigator	P34243.
GermOnline	YKL017C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR003593. AAA+_ATPase. IPR004483. DNA_helicase_put. IPR014001. Helicase_ATP-bd. [Graphical view]
SMART	SM00382. AAA. 1 hit. SM00487. DEXDc. 1 hit. [Graphical view]
TIGRFAMs	TIGR00376. TIGR00376. 1 hit.
ProtoNet	Search...
Other
NextBio	975085.

Entry information

Entry name

HCS1_YEAST

Accession

Primary (citable) accession number: P34243
Secondary accession number(s): D6VXR9

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1994
Last sequence update:	February 1, 1994
Last modified:	May 1, 2013
This is version 102 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program