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P34243 (HCS1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 111. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
DNA polymerase alpha-associated DNA helicase A

EC=3.6.4.12
Gene names
Name:HCS1
Synonyms:DIP1
Ordered Locus Names:YKL017C
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length683 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

DNA polymerase alpha-associated DNA helicase which may be involved in DNA replication. Ref.4 Ref.5 Ref.6 Ref.7 Ref.8

Catalytic activity

ATP + H2O = ADP + phosphate.

Subunit structure

Associates with the hexameric DNA polymerase alpha.

Subcellular location

Cytoplasm. Nucleus Probable Ref.10.

Induction

By heat shock. Ref.9

Miscellaneous

Present with 1040 molecules/cell in log phase SD medium.

Sequence similarities

Belongs to the DNA2/NAM7 helicase family.

Biophysicochemical properties

Kinetic parameters:

KM=90 µM for ATP Ref.4 Ref.8

Vmax=14 µmol/min/mg enzyme

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 683683DNA polymerase alpha-associated DNA helicase A
PRO_0000080726

Regions

Nucleotide binding229 – 2368ATP Potential

Sequences

Sequence LengthMass (Da)Tools
P34243 [UniParc].

Last modified February 1, 1994. Version 1.
Checksum: FCD02680757377B8

FASTA68378,273
        10         20         30         40         50         60 
MNKELASKFL SSIKHEREQD IQTTSRLLTT LSIQQLVQNG LAINNIHLEN IRSGLIGKLY 

        70         80         90        100        110        120 
MELGPNLAVN DKIQRGDIKV GDIVLVRPAK TKVNTKTKPK VKKVSEDSNG EQAECSGVVY 

       130        140        150        160        170        180 
KMSDTQITIA LEESQDVIAT TFYSYSKLYI LKTTNVVTYN RMESTMRKLS EISSPIQDKI 

       190        200        210        220        230        240 
IQYLVNERPF IPNTNSFQNI KSFLNPNLND SQKTAINFAI NNDLTIIHGP PGTGKTFTLI 

       250        260        270        280        290        300 
ELIQQLLIKN PEERILICGP SNISVDTILE RLTPLVPNNL LLRIGHPARL LDSNKRHSLD 

       310        320        330        340        350        360 
ILSKKNTIVK DISQEIDKLI QENKKLKNYK QRKENWNEIK LLRKDLKKRE FKTIKDLIIQ 

       370        380        390        400        410        420 
SRIVVTTLHG SSSRELCSLY RDDPNFQLFD TLIIDEVSQA MEPQCWIPLI AHQNQFHKLV 

       430        440        450        460        470        480 
LAGDNKQLPP TIKTEDDKNV IHNLETTLFD RIIKIFPKRD MVKFLNVQYR MNQKIMEFPS 

       490        500        510        520        530        540 
HSMYNGKLLA DATVANRLLI DLPTVDATPS EDDDDTKIPL IWYDTQGDEF QETADEATIL 

       550        560        570        580        590        600 
GSKYNEGEIA IVKEHIENLR SFNVPENSIG VISPYNAQVS HLKKLIHDEL KLTDIEISTV 

       610        620        630        640        650        660 
DGFQGREKDV IILSLVRSNE KFEVGFLKEE RRLNVAMTRP RRQLVVVGNI EVLQRCGNKY 

       670        680 
LKSWSEWCEE NADVRYPNID DYL 

« Hide

References

« Hide 'large scale' references
[1]"Sequencing and analysis of 51.6 kilobases on the left arm of chromosome XI from Saccharomyces cerevisiae reveals 23 open reading frames including the FAS1 gene."
Wiemann S., Voss H., Schwager C., Rupp T., Stegemann J., Zimmermann J., Grothues D., Sensen C., Erfle H., Hewitt N., Banrevi A., Ansorge W.
Yeast 9:1343-1348(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"Complete DNA sequence of yeast chromosome XI."
Dujon B., Alexandraki D., Andre B., Ansorge W., Baladron V., Ballesta J.P.G., Banrevi A., Bolle P.-A., Bolotin-Fukuhara M., Bossier P., Bou G., Boyer J., Buitrago M.J., Cheret G., Colleaux L., Daignan-Fornier B., del Rey F., Dion C. expand/collapse author list , Domdey H., Duesterhoeft A., Duesterhus S., Entian K.-D., Erfle H., Esteban P.F., Feldmann H., Fernandes L., Fobo G.M., Fritz C., Fukuhara H., Gabel C., Gaillon L., Garcia-Cantalejo J.M., Garcia-Ramirez J.J., Gent M.E., Ghazvini M., Goffeau A., Gonzalez A., Grothues D., Guerreiro P., Hegemann J.H., Hewitt N., Hilger F., Hollenberg C.P., Horaitis O., Indge K.J., Jacquier A., James C.M., Jauniaux J.-C., Jimenez A., Keuchel H., Kirchrath L., Kleine K., Koetter P., Legrain P., Liebl S., Louis E.J., Maia e Silva A., Marck C., Monnier A.-L., Moestl D., Mueller S., Obermaier B., Oliver S.G., Pallier C., Pascolo S., Pfeiffer F., Philippsen P., Planta R.J., Pohl F.M., Pohl T.M., Poehlmann R., Portetelle D., Purnelle B., Puzos V., Ramezani Rad M., Rasmussen S.W., Remacha M.A., Revuelta J.L., Richard G.-F., Rieger M., Rodrigues-Pousada C., Rose M., Rupp T., Santos M.A., Schwager C., Sensen C., Skala J., Soares H., Sor F., Stegemann J., Tettelin H., Thierry A., Tzermia M., Urrestarazu L.A., van Dyck L., van Vliet-Reedijk J.C., Valens M., Vandenbol M., Vilela C., Vissers S., von Wettstein D., Voss H., Wiemann S., Xu G., Zimmermann J., Haasemann M., Becker I., Mewes H.-W.
Nature 369:371-378(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[3]"The reference genome sequence of Saccharomyces cerevisiae: Then and now."
Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R., Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S., Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.
G3 (Bethesda) 4:389-398(2014) [PubMed] [Europe PMC] [Abstract]
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[4]"Purification and characterization of DNA polymerase alpha-associated replication protein A-dependent yeast DNA helicase A."
Biswas S.B., Chen P.H., Biswas E.E.
Biochemistry 36:13270-13276(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 9-22, FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES.
[5]"Purification and characterization of a yeast DNA polymerase alpha complex with associated primase, 5'-->3' exonuclease, and DNA-dependent ATPase activities."
Biswas E.E., Chen P.H., Gray W., Li Y.H., Ray S., Biswas S.B.
Biochemistry 32:3013-3019(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, ASSOCIATION WITH DNA POLYMERASE ALPHA.
[6]"Characterization of the DNA-dependent ATPase and a DNA unwinding activity associated with the yeast DNA polymerase alpha complex."
Biswas E.E., Ewing C.M., Biswas S.B.
Biochemistry 32:3020-3026(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, ASSOCIATION WITH DNA POLYMERASE ALPHA.
[7]"DNA helicase associated with DNA polymerase alpha: isolation by a modified immunoaffinity chromatography."
Biswas E.E., Chen P.H., Biswas S.B.
Biochemistry 32:13393-13398(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, ASSOCIATION WITH DNA POLYMERASE ALPHA.
[8]"Yeast DNA helicase A: cloning, expression, purification, and enzymatic characterization."
Biswas E.E., Fricke W.M., Chen P.H., Biswas S.B.
Biochemistry 36:13277-13284(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES.
[9]"Systematic identification, classification, and characterization of the open reading frames which encode novel helicase-related proteins in Saccharomyces cerevisiae by gene disruption and Northern analysis."
Shiratori A., Shibata T., Arisawa M., Hanaoka F., Murakami Y., Eki T.
Yeast 15:219-253(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: INDUCTION.
[10]"Global analysis of protein localization in budding yeast."
Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K.
Nature 425:686-691(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[11]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X74152 Genomic DNA. Translation: CAA52266.1.
Z28017 Genomic DNA. Translation: CAA81852.1.
BK006944 Genomic DNA. Translation: DAA09139.1.
PIRS34700.
RefSeqNP_012908.1. NM_001179583.1.

3D structure databases

ProteinModelPortalP34243.
SMRP34243. Positions 117-655.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid34115. 9 interactions.
DIPDIP-1383N.
IntActP34243. 1 interaction.
MINTMINT-399891.
STRING4932.YKL017C.

Proteomic databases

MaxQBP34243.
PaxDbP34243.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYKL017C; YKL017C; YKL017C.
GeneID853852.
KEGGsce:YKL017C.

Organism-specific databases

CYGDYKL017c.
SGDS000001500. HCS1.

Phylogenomic databases

eggNOGCOG1112.
GeneTreeENSGT00730000111172.
HOGENOMHOG000185831.
OMAFQGRENE.
OrthoDBEOG70PC5T.

Enzyme and pathway databases

BioCycYEAST:G3O-31826-MONOMER.
SABIO-RKP34243.

Gene expression databases

GenevestigatorP34243.

Family and domain databases

Gene3D3.40.50.300. 3 hits.
InterProIPR003593. AAA+_ATPase.
IPR014001. Helicase_ATP-bd.
IPR027417. P-loop_NTPase.
IPR004483. SMUBP-2/Hcs1_like.
[Graphical view]
SMARTSM00382. AAA. 1 hit.
SM00487. DEXDc. 1 hit.
[Graphical view]
SUPFAMSSF52540. SSF52540. 1 hit.
TIGRFAMsTIGR00376. TIGR00376. 1 hit.
ProtoNetSearch...

Other

NextBio975085.
PROP34243.

Entry information

Entry nameHCS1_YEAST
AccessionPrimary (citable) accession number: P34243
Secondary accession number(s): D6VXR9
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: February 1, 1994
Last modified: June 11, 2014
This is version 111 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome XI

Yeast (Saccharomyces cerevisiae) chromosome XI: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families