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P34241

- URB1_YEAST

UniProt

P34241 - URB1_YEAST

Protein

Nucleolar pre-ribosomal-associated protein 1

Gene

URB1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 92 (01 Oct 2014)
      Sequence version 2 (01 Jun 1994)
      Previous versions | rss
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    Functioni

    Required for 60S ribosomal subunit formation and pre-rRNA processing. Required for normal accumulation of 25S and 5.8S rRNAs.3 Publications

    GO - Biological processi

    1. maturation of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) Source: SGD
    2. maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) Source: SGD

    Keywords - Biological processi

    Ribosome biogenesis, rRNA processing

    Enzyme and pathway databases

    BioCyciYEAST:G3O-31823-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Nucleolar pre-ribosomal-associated protein 1
    Alternative name(s):
    Unhealthy ribosome biogenesis protein 1
    Gene namesi
    Name:URB1
    Synonyms:NPA1
    Ordered Locus Names:YKL014C
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome XI

    Organism-specific databases

    CYGDiYKL014c.
    SGDiS000001497. URB1.

    Subcellular locationi

    Nucleusnucleolus 3 Publications
    Note: Accumulates in the immediate vicinity of the dense fibrillar component of the nucleolus.

    GO - Cellular componenti

    1. nucleolus Source: SGD

    Keywords - Cellular componenti

    Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 17641764Nucleolar pre-ribosomal-associated protein 1PRO_0000203190Add
    BLAST

    Proteomic databases

    MaxQBiP34241.
    PaxDbiP34241.
    PeptideAtlasiP34241.

    Expressioni

    Gene expression databases

    GenevestigatoriP34241.

    Interactioni

    Subunit structurei

    Associates with pre-60S ribosomal particles. Predominantly associated with the 27SA2 pre-rRNA. Can associate with a subset of box H/ACA and box C/D small nucleolar RNPs (snoRNPs) required for peptidyl transferase center modification and with small RNAs snR37 and snR42. Interacts with URB2. Together with DBP6, NOP8, URB2 and RSA3, forms an RNA-independent complex, which is required during early maturation of nascent 60S ribosomal subunits.3 Publications

    Protein-protein interaction databases

    BioGridi34118. 99 interactions.
    DIPiDIP-2701N.
    IntActiP34241. 17 interactions.
    MINTiMINT-668747.
    STRINGi4932.YKL014C.

    Structurei

    3D structure databases

    ProteinModelPortaliP34241.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Phylogenomic databases

    eggNOGiNOG324825.
    HOGENOMiHOG000141826.
    KOiK14861.
    OMAiPYKYVDM.
    OrthoDBiEOG76DV31.

    Family and domain databases

    InterProiIPR021714. Npa1_N.
    [Graphical view]
    PfamiPF11707. Npa1. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P34241-1 [UniParc]FASTAAdd to Basket

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    MSNHSEAYGS RDQRREKYTQ GKEFEDGTLE TLESIISAVE DETLSKDYQP     50
    LIVFFQRGFG AQLVQTWSYY AQVNNHGKFS KTTSLLTKTL RVLSSDTSTV 100
    TIGSGLIRLI LTDYTKVLYR GLNNMRAQLT NPILRLLKQI VNFNNGQHIE 150
    ELVSYFDFSL PILPRLLVPS KSELANGNSS ADSSKHDSLR FTFIKFWLTL 200
    ISNASPFVRK ELLTENFKIM SNLFKFMNKA DSDKLSEHIL SVFINDILKE 250
    KSFKRTTKTK ILNELAASKI HHFYYSSNKN LVKKANEFFL TFGASRDFSV 300
    AFPDNCVWFK NSVADGASHG APITVNQVEF QIHNKLLFNT LRLFKPWEDT 350
    LQLGTLIKIL ENVPELVAPY SIFLTTNGNY DPKMTSYWFG ITLLINKIIN 400
    LKIPQFMEKV DSNIPPATSL VIENILPSLL TKSSLVKSLQ FETPIIRQLA 450
    CQSIVLALKK LEKVSTFYDQ KGWRNEKTIL LNEFHTRIPD LPIFVSTLSN 500
    SLASNKDNRI LPLSISIIFN YYSKMFPNLF SINLPSSNIY TDIMQKSKIS 550
    GIEFAILDNY LQFQEFNSTQ TRWWNPSSGG NSLFTLLLKL ASSKNASNVI 600
    TTRISNLLDE LTRTNVIFNI SLISPVMALV NSLQGLSLQV SEIDNMEQVW 650
    KWFDETISRV VKTPYKYVDM AKEYNYISPF IMCLSEQWKY VDKSGNPEFL 700
    IKWLILFLRN MIFIGEDHIG IDKLVKNVFP EVSDHDVNIY LKLDSFEENI 750
    KKTNSSNSLI SSMKSSSFFQ YISALPSKNL MNISRLPVNK LDAAGILFRV 800
    QLLVEDDSVV YDNWFEATAC ELTGKIASYM VTDTEFPIIK VLERYINFAL 850
    PKLAIEKRNA LLMKKSRFMC NLIGAVCFET GHQLVEFREI IQKVVFSGEN 900
    VEEYANYNEL YQKEDVNAFL TSVSEYLSTS ALTSLLMCST KLESTRNILQ 950
    KLFNEGKTIK ISLVKNILNK AANEDPASIK EVNISLAKFF EENKVCVDAS 1000
    SDPMGKLSLS ETTSLINSFV SSDLNYLVLK AFYRWEHFSF PSFIPSIWRI 1050
    KDSPLLSIVT TAALFKHMQD KDFSAFAHET ISKYGNEIAK STYTTSKSEI 1100
    FDEILNMITT YIDFYDETKR NEILKCVLSQ SDHKYHAATV RYIAAHNNFT 1150
    YPGVETWLHK TLLYLTKYLS ERKVISNSFF ELLRAMAELL KLEEVPNKLN 1200
    VKIINSQLEA ILGSEWIKQI KVLEYVIVLI FCVSKKSIQS QRMVQLLLSN 1250
    DSYSSIMIKD NDEDSSYRKF LSTMILFSLF SIDPVVNSTP IVQEKLLTFY 1300
    SGTISSNDKL ILKILETIES HTATSWTNMI FSWEFIKDEE EEILEAIGDT 1350
    RLITKEREGL ILTLQKNMIK KSIDRYVLER PQVPELYTDS NTNNYDATTR 1400
    CDLVKKYYDD TERSGVDMYD PLFLLLLIIH NKELVKMVKD DEGNVTYRYE 1450
    FENFLDCKIF QFIICSLSDC HTVANISYEH LSNLASSLEK KTAQMNLEKQ 1500
    ITSKDNERKE SDSDLIKYNS IYQVLIKRIL YQRQQNQDPI NPLIWFSISR 1550
    IVDLLGSPTA PLHEKAYRWV LSNSTIRSWD IPMVSDVMMS YNKRQQDDNK 1600
    KEIDMEIYYG ELSWVLTTIC KGIKTDEDYK MLEKKGVFEW LLNLINMPYL 1650
    KERLRELIYF IFYKVQRVAD DGGLNLISRN GIVSFFEVLN NNIKSRLPQD 1700
    DILNNIGTLR NENRGTLNTT LRLAQEQNGI EKLLLGYNEL VKSQKRLILW 1750
    TEGDSDNVVK RLRK 1764
    Length:1,764
    Mass (Da):203,287
    Last modified:June 1, 1994 - v2
    Checksum:iD4EC1FA19E051367
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X74152 Genomic DNA. Translation: CAA52264.1.
    Z28014 Genomic DNA. Translation: CAA81849.1.
    BK006944 Genomic DNA. Translation: DAA09142.1.
    PIRiS37827.
    RefSeqiNP_012911.3. NM_001179580.3.

    Genome annotation databases

    EnsemblFungiiYKL014C; YKL014C; YKL014C.
    GeneIDi853855.
    KEGGisce:YKL014C.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X74152 Genomic DNA. Translation: CAA52264.1 .
    Z28014 Genomic DNA. Translation: CAA81849.1 .
    BK006944 Genomic DNA. Translation: DAA09142.1 .
    PIRi S37827.
    RefSeqi NP_012911.3. NM_001179580.3.

    3D structure databases

    ProteinModelPortali P34241.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 34118. 99 interactions.
    DIPi DIP-2701N.
    IntActi P34241. 17 interactions.
    MINTi MINT-668747.
    STRINGi 4932.YKL014C.

    Proteomic databases

    MaxQBi P34241.
    PaxDbi P34241.
    PeptideAtlasi P34241.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YKL014C ; YKL014C ; YKL014C .
    GeneIDi 853855.
    KEGGi sce:YKL014C.

    Organism-specific databases

    CYGDi YKL014c.
    SGDi S000001497. URB1.

    Phylogenomic databases

    eggNOGi NOG324825.
    HOGENOMi HOG000141826.
    KOi K14861.
    OMAi PYKYVDM.
    OrthoDBi EOG76DV31.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-31823-MONOMER.

    Miscellaneous databases

    NextBioi 975094.

    Gene expression databases

    Genevestigatori P34241.

    Family and domain databases

    InterProi IPR021714. Npa1_N.
    [Graphical view ]
    Pfami PF11707. Npa1. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Sequencing and analysis of 51.6 kilobases on the left arm of chromosome XI from Saccharomyces cerevisiae reveals 23 open reading frames including the FAS1 gene."
      Wiemann S., Voss H., Schwager C., Rupp T., Stegemann J., Zimmermann J., Grothues D., Sensen C., Erfle H., Hewitt N., Banrevi A., Ansorge W.
      Yeast 9:1343-1348(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. "Complete DNA sequence of yeast chromosome XI."
      Dujon B., Alexandraki D., Andre B., Ansorge W., Baladron V., Ballesta J.P.G., Banrevi A., Bolle P.-A., Bolotin-Fukuhara M., Bossier P., Bou G., Boyer J., Buitrago M.J., Cheret G., Colleaux L., Daignan-Fornier B., del Rey F., Dion C.
      , Domdey H., Duesterhoeft A., Duesterhus S., Entian K.-D., Erfle H., Esteban P.F., Feldmann H., Fernandes L., Fobo G.M., Fritz C., Fukuhara H., Gabel C., Gaillon L., Garcia-Cantalejo J.M., Garcia-Ramirez J.J., Gent M.E., Ghazvini M., Goffeau A., Gonzalez A., Grothues D., Guerreiro P., Hegemann J.H., Hewitt N., Hilger F., Hollenberg C.P., Horaitis O., Indge K.J., Jacquier A., James C.M., Jauniaux J.-C., Jimenez A., Keuchel H., Kirchrath L., Kleine K., Koetter P., Legrain P., Liebl S., Louis E.J., Maia e Silva A., Marck C., Monnier A.-L., Moestl D., Mueller S., Obermaier B., Oliver S.G., Pallier C., Pascolo S., Pfeiffer F., Philippsen P., Planta R.J., Pohl F.M., Pohl T.M., Poehlmann R., Portetelle D., Purnelle B., Puzos V., Ramezani Rad M., Rasmussen S.W., Remacha M.A., Revuelta J.L., Richard G.-F., Rieger M., Rodrigues-Pousada C., Rose M., Rupp T., Santos M.A., Schwager C., Sensen C., Skala J., Soares H., Sor F., Stegemann J., Tettelin H., Thierry A., Tzermia M., Urrestarazu L.A., van Dyck L., van Vliet-Reedijk J.C., Valens M., Vandenbol M., Vilela C., Vissers S., von Wettstein D., Voss H., Wiemann S., Xu G., Zimmermann J., Haasemann M., Becker I., Mewes H.-W.
      Nature 369:371-378(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    3. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    4. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
    5. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    6. Cited for: FUNCTION, INTERACTION WITH URB2.
    7. "Npa1p, a component of very early pre-60S ribosomal particles, associates with a subset of small nucleolar RNPs required for peptidyl transferase center modification."
      Dez C., Froment C., Noaillac-Depeyre J., Monsarrat B., Caizergues-Ferrer M., Henry Y.
      Mol. Cell. Biol. 24:6324-6337(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, SUBUNIT, INTERACTION WITH URB2, SUBCELLULAR LOCATION.
    8. "Npa1p is an essential trans-acting factor required for an early step in the assembly of 60S ribosomal subunits in Saccharomyces cerevisiae."
      Rosado I.V., de la Cruz J.
      RNA 10:1073-1083(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, ASSOCIATION WITH PRE-RIBOSOMAL PARTICLES, SUBCELLULAR LOCATION.
    9. "Characterization of Saccharomyces cerevisiae Npa2p (Urb2p) reveals a Low-molecular-mass complex containing Dbp6p, Npa1p (Urb1p), Nop8p, and Rsa3p involved in early steps of 60S ribosomal subunit biogenesis."
      Rosado I.V., Dez C., Lebaron S., Caizergues-Ferrer M., Henry Y., de la Cruz J.
      Mol. Cell. Biol. 27:1207-1221(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION IN A COMPLEX WITH DBP6; NOP8; URB2 AND RSA3.

    Entry informationi

    Entry nameiURB1_YEAST
    AccessioniPrimary (citable) accession number: P34241
    Secondary accession number(s): D6VXS2, P34242
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1994
    Last sequence update: June 1, 1994
    Last modified: October 1, 2014
    This is version 92 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 5890 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    2. Yeast chromosome XI
      Yeast (Saccharomyces cerevisiae) chromosome XI: entries and gene names

    External Data

    Dasty 3