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P34241 (URB1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 91. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Nucleolar pre-ribosomal-associated protein 1
Alternative name(s):
Unhealthy ribosome biogenesis protein 1
Gene names
Name:URB1
Synonyms:NPA1
Ordered Locus Names:YKL014C
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length1764 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Required for 60S ribosomal subunit formation and pre-rRNA processing. Required for normal accumulation of 25S and 5.8S rRNAs. Ref.6 Ref.7 Ref.8

Subunit structure

Associates with pre-60S ribosomal particles. Predominantly associated with the 27SA2 pre-rRNA. Can associate with a subset of box H/ACA and box C/D small nucleolar RNPs (snoRNPs) required for peptidyl transferase center modification and with small RNAs snR37 and snR42. Interacts with URB2. Together with DBP6, NOP8, URB2 and RSA3, forms an RNA-independent complex, which is required during early maturation of nascent 60S ribosomal subunits. Ref.6 Ref.7 Ref.9

Subcellular location

Nucleusnucleolus. Note: Accumulates in the immediate vicinity of the dense fibrillar component of the nucleolus. Ref.4 Ref.7 Ref.8

Miscellaneous

Present with 5890 molecules/cell in log phase SD medium.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 17641764Nucleolar pre-ribosomal-associated protein 1
PRO_0000203190

Sequences

Sequence LengthMass (Da)Tools
P34241 [UniParc].

Last modified June 1, 1994. Version 2.
Checksum: D4EC1FA19E051367

FASTA1,764203,287
        10         20         30         40         50         60 
MSNHSEAYGS RDQRREKYTQ GKEFEDGTLE TLESIISAVE DETLSKDYQP LIVFFQRGFG 

        70         80         90        100        110        120 
AQLVQTWSYY AQVNNHGKFS KTTSLLTKTL RVLSSDTSTV TIGSGLIRLI LTDYTKVLYR 

       130        140        150        160        170        180 
GLNNMRAQLT NPILRLLKQI VNFNNGQHIE ELVSYFDFSL PILPRLLVPS KSELANGNSS 

       190        200        210        220        230        240 
ADSSKHDSLR FTFIKFWLTL ISNASPFVRK ELLTENFKIM SNLFKFMNKA DSDKLSEHIL 

       250        260        270        280        290        300 
SVFINDILKE KSFKRTTKTK ILNELAASKI HHFYYSSNKN LVKKANEFFL TFGASRDFSV 

       310        320        330        340        350        360 
AFPDNCVWFK NSVADGASHG APITVNQVEF QIHNKLLFNT LRLFKPWEDT LQLGTLIKIL 

       370        380        390        400        410        420 
ENVPELVAPY SIFLTTNGNY DPKMTSYWFG ITLLINKIIN LKIPQFMEKV DSNIPPATSL 

       430        440        450        460        470        480 
VIENILPSLL TKSSLVKSLQ FETPIIRQLA CQSIVLALKK LEKVSTFYDQ KGWRNEKTIL 

       490        500        510        520        530        540 
LNEFHTRIPD LPIFVSTLSN SLASNKDNRI LPLSISIIFN YYSKMFPNLF SINLPSSNIY 

       550        560        570        580        590        600 
TDIMQKSKIS GIEFAILDNY LQFQEFNSTQ TRWWNPSSGG NSLFTLLLKL ASSKNASNVI 

       610        620        630        640        650        660 
TTRISNLLDE LTRTNVIFNI SLISPVMALV NSLQGLSLQV SEIDNMEQVW KWFDETISRV 

       670        680        690        700        710        720 
VKTPYKYVDM AKEYNYISPF IMCLSEQWKY VDKSGNPEFL IKWLILFLRN MIFIGEDHIG 

       730        740        750        760        770        780 
IDKLVKNVFP EVSDHDVNIY LKLDSFEENI KKTNSSNSLI SSMKSSSFFQ YISALPSKNL 

       790        800        810        820        830        840 
MNISRLPVNK LDAAGILFRV QLLVEDDSVV YDNWFEATAC ELTGKIASYM VTDTEFPIIK 

       850        860        870        880        890        900 
VLERYINFAL PKLAIEKRNA LLMKKSRFMC NLIGAVCFET GHQLVEFREI IQKVVFSGEN 

       910        920        930        940        950        960 
VEEYANYNEL YQKEDVNAFL TSVSEYLSTS ALTSLLMCST KLESTRNILQ KLFNEGKTIK 

       970        980        990       1000       1010       1020 
ISLVKNILNK AANEDPASIK EVNISLAKFF EENKVCVDAS SDPMGKLSLS ETTSLINSFV 

      1030       1040       1050       1060       1070       1080 
SSDLNYLVLK AFYRWEHFSF PSFIPSIWRI KDSPLLSIVT TAALFKHMQD KDFSAFAHET 

      1090       1100       1110       1120       1130       1140 
ISKYGNEIAK STYTTSKSEI FDEILNMITT YIDFYDETKR NEILKCVLSQ SDHKYHAATV 

      1150       1160       1170       1180       1190       1200 
RYIAAHNNFT YPGVETWLHK TLLYLTKYLS ERKVISNSFF ELLRAMAELL KLEEVPNKLN 

      1210       1220       1230       1240       1250       1260 
VKIINSQLEA ILGSEWIKQI KVLEYVIVLI FCVSKKSIQS QRMVQLLLSN DSYSSIMIKD 

      1270       1280       1290       1300       1310       1320 
NDEDSSYRKF LSTMILFSLF SIDPVVNSTP IVQEKLLTFY SGTISSNDKL ILKILETIES 

      1330       1340       1350       1360       1370       1380 
HTATSWTNMI FSWEFIKDEE EEILEAIGDT RLITKEREGL ILTLQKNMIK KSIDRYVLER 

      1390       1400       1410       1420       1430       1440 
PQVPELYTDS NTNNYDATTR CDLVKKYYDD TERSGVDMYD PLFLLLLIIH NKELVKMVKD 

      1450       1460       1470       1480       1490       1500 
DEGNVTYRYE FENFLDCKIF QFIICSLSDC HTVANISYEH LSNLASSLEK KTAQMNLEKQ 

      1510       1520       1530       1540       1550       1560 
ITSKDNERKE SDSDLIKYNS IYQVLIKRIL YQRQQNQDPI NPLIWFSISR IVDLLGSPTA 

      1570       1580       1590       1600       1610       1620 
PLHEKAYRWV LSNSTIRSWD IPMVSDVMMS YNKRQQDDNK KEIDMEIYYG ELSWVLTTIC 

      1630       1640       1650       1660       1670       1680 
KGIKTDEDYK MLEKKGVFEW LLNLINMPYL KERLRELIYF IFYKVQRVAD DGGLNLISRN 

      1690       1700       1710       1720       1730       1740 
GIVSFFEVLN NNIKSRLPQD DILNNIGTLR NENRGTLNTT LRLAQEQNGI EKLLLGYNEL 

      1750       1760 
VKSQKRLILW TEGDSDNVVK RLRK 

« Hide

References

« Hide 'large scale' references
[1]"Sequencing and analysis of 51.6 kilobases on the left arm of chromosome XI from Saccharomyces cerevisiae reveals 23 open reading frames including the FAS1 gene."
Wiemann S., Voss H., Schwager C., Rupp T., Stegemann J., Zimmermann J., Grothues D., Sensen C., Erfle H., Hewitt N., Banrevi A., Ansorge W.
Yeast 9:1343-1348(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"Complete DNA sequence of yeast chromosome XI."
Dujon B., Alexandraki D., Andre B., Ansorge W., Baladron V., Ballesta J.P.G., Banrevi A., Bolle P.-A., Bolotin-Fukuhara M., Bossier P., Bou G., Boyer J., Buitrago M.J., Cheret G., Colleaux L., Daignan-Fornier B., del Rey F., Dion C. expand/collapse author list , Domdey H., Duesterhoeft A., Duesterhus S., Entian K.-D., Erfle H., Esteban P.F., Feldmann H., Fernandes L., Fobo G.M., Fritz C., Fukuhara H., Gabel C., Gaillon L., Garcia-Cantalejo J.M., Garcia-Ramirez J.J., Gent M.E., Ghazvini M., Goffeau A., Gonzalez A., Grothues D., Guerreiro P., Hegemann J.H., Hewitt N., Hilger F., Hollenberg C.P., Horaitis O., Indge K.J., Jacquier A., James C.M., Jauniaux J.-C., Jimenez A., Keuchel H., Kirchrath L., Kleine K., Koetter P., Legrain P., Liebl S., Louis E.J., Maia e Silva A., Marck C., Monnier A.-L., Moestl D., Mueller S., Obermaier B., Oliver S.G., Pallier C., Pascolo S., Pfeiffer F., Philippsen P., Planta R.J., Pohl F.M., Pohl T.M., Poehlmann R., Portetelle D., Purnelle B., Puzos V., Ramezani Rad M., Rasmussen S.W., Remacha M.A., Revuelta J.L., Richard G.-F., Rieger M., Rodrigues-Pousada C., Rose M., Rupp T., Santos M.A., Schwager C., Sensen C., Skala J., Soares H., Sor F., Stegemann J., Tettelin H., Thierry A., Tzermia M., Urrestarazu L.A., van Dyck L., van Vliet-Reedijk J.C., Valens M., Vandenbol M., Vilela C., Vissers S., von Wettstein D., Voss H., Wiemann S., Xu G., Zimmermann J., Haasemann M., Becker I., Mewes H.-W.
Nature 369:371-378(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[3]"The reference genome sequence of Saccharomyces cerevisiae: Then and now."
Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R., Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S., Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.
G3 (Bethesda) 4:389-398(2014) [PubMed] [Europe PMC] [Abstract]
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[4]"Global analysis of protein localization in budding yeast."
Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K.
Nature 425:686-691(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[5]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[6]"Exploration of essential gene functions via titratable promoter alleles."
Mnaimneh S., Davierwala A.P., Haynes J., Moffat J., Peng W.-T., Zhang W., Yang X., Pootoolal J., Chua G., Lopez A., Trochesset M., Morse D., Krogan N.J., Hiley S.L., Li Z., Morris Q., Grigull J., Mitsakakis N. expand/collapse author list , Roberts C.J., Greenblatt J.F., Boone C., Kaiser C.A., Andrews B.J., Hughes T.R.
Cell 118:31-44(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, INTERACTION WITH URB2.
[7]"Npa1p, a component of very early pre-60S ribosomal particles, associates with a subset of small nucleolar RNPs required for peptidyl transferase center modification."
Dez C., Froment C., Noaillac-Depeyre J., Monsarrat B., Caizergues-Ferrer M., Henry Y.
Mol. Cell. Biol. 24:6324-6337(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, SUBUNIT, INTERACTION WITH URB2, SUBCELLULAR LOCATION.
[8]"Npa1p is an essential trans-acting factor required for an early step in the assembly of 60S ribosomal subunits in Saccharomyces cerevisiae."
Rosado I.V., de la Cruz J.
RNA 10:1073-1083(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, ASSOCIATION WITH PRE-RIBOSOMAL PARTICLES, SUBCELLULAR LOCATION.
[9]"Characterization of Saccharomyces cerevisiae Npa2p (Urb2p) reveals a Low-molecular-mass complex containing Dbp6p, Npa1p (Urb1p), Nop8p, and Rsa3p involved in early steps of 60S ribosomal subunit biogenesis."
Rosado I.V., Dez C., Lebaron S., Caizergues-Ferrer M., Henry Y., de la Cruz J.
Mol. Cell. Biol. 27:1207-1221(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION IN A COMPLEX WITH DBP6; NOP8; URB2 AND RSA3.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X74152 Genomic DNA. Translation: CAA52264.1.
Z28014 Genomic DNA. Translation: CAA81849.1.
BK006944 Genomic DNA. Translation: DAA09142.1.
PIRS37827.
RefSeqNP_012911.3. NM_001179580.3.

3D structure databases

ProteinModelPortalP34241.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid34118. 99 interactions.
DIPDIP-2701N.
IntActP34241. 17 interactions.
MINTMINT-668747.
STRING4932.YKL014C.

Proteomic databases

MaxQBP34241.
PaxDbP34241.
PeptideAtlasP34241.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYKL014C; YKL014C; YKL014C.
GeneID853855.
KEGGsce:YKL014C.

Organism-specific databases

CYGDYKL014c.
SGDS000001497. URB1.

Phylogenomic databases

eggNOGNOG324825.
HOGENOMHOG000141826.
KOK14861.
OMAPYKYVDM.
OrthoDBEOG76DV31.

Enzyme and pathway databases

BioCycYEAST:G3O-31823-MONOMER.

Gene expression databases

GenevestigatorP34241.

Family and domain databases

InterProIPR021714. Npa1_N.
[Graphical view]
PfamPF11707. Npa1. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio975094.

Entry information

Entry nameURB1_YEAST
AccessionPrimary (citable) accession number: P34241
Secondary accession number(s): D6VXS2, P34242
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: June 1, 1994
Last modified: May 14, 2014
This is version 91 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome XI

Yeast (Saccharomyces cerevisiae) chromosome XI: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD