P34241 (URB1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Nucleolar pre-ribosomal-associated protein 1

Alternative name(s):
Unhealthy ribosome biogenesis protein 1

Gene names

Name:	URB1
Synonyms:	NPA1
Ordered Locus Names:	YKL014C

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › Saccharomyces cerevisiae

Protein attributes

Sequence length	1764 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Required for 60S ribosomal subunit formation and pre-rRNA processing. Required for normal accumulation of 25S and 5.8S rRNAs. Ref.6 Ref.7 Ref.8
Subunit structure	Associates with pre-60S ribosomal particles. Predominantly associated with the 27SA2 pre-rRNA. Can associate with a subset of box H/ACA and box C/D small nucleolar RNPs (snoRNPs) required for peptidyl transferase center modification and with small RNAs snR37 and snR42. Interacts with URB2. Together with DBP6, NOP8, URB2 and RSA3, forms an RNA-independent complex, which is required during early maturation of nascent 60S ribosomal subunits. Ref.6 Ref.7 Ref.9
Subcellular location	Nucleus › nucleolus. Note: Accumulates in the immediate vicinity of the dense fibrillar component of the nucleolus. Ref.4 Ref.7 Ref.8
Miscellaneous	Present with 5890 molecules/cell in log phase SD medium.

Ontologies

Keywords
Biological process	Ribosome biogenesis rRNA processing
Cellular component	Nucleus
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	maturation of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) Inferred from mutant phenotype Ref.8. Source: SGD maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) Inferred from mutant phenotype Ref.8. Source: SGD
Cellular_component	nucleolus Inferred from direct assay Ref.4Ref.8PubMed 22842922. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1764	1764	Nucleolar pre-ribosomal-associated protein 1		PRO_0000203190

Sequences

	Sequence		Length	Mass (Da)
	P34241 [UniParc]. Last modified June 1, 1994. Version 2. Checksum: D4EC1FA19E051367	FASTA	1,764	203,287
	10 20 30 40 50 60 MSNHSEAYGS RDQRREKYTQ GKEFEDGTLE TLESIISAVE DETLSKDYQP LIVFFQRGFG 70 80 90 100 110 120 AQLVQTWSYY AQVNNHGKFS KTTSLLTKTL RVLSSDTSTV TIGSGLIRLI LTDYTKVLYR 130 140 150 160 170 180 GLNNMRAQLT NPILRLLKQI VNFNNGQHIE ELVSYFDFSL PILPRLLVPS KSELANGNSS 190 200 210 220 230 240 ADSSKHDSLR FTFIKFWLTL ISNASPFVRK ELLTENFKIM SNLFKFMNKA DSDKLSEHIL 250 260 270 280 290 300 SVFINDILKE KSFKRTTKTK ILNELAASKI HHFYYSSNKN LVKKANEFFL TFGASRDFSV 310 320 330 340 350 360 AFPDNCVWFK NSVADGASHG APITVNQVEF QIHNKLLFNT LRLFKPWEDT LQLGTLIKIL 370 380 390 400 410 420 ENVPELVAPY SIFLTTNGNY DPKMTSYWFG ITLLINKIIN LKIPQFMEKV DSNIPPATSL 430 440 450 460 470 480 VIENILPSLL TKSSLVKSLQ FETPIIRQLA CQSIVLALKK LEKVSTFYDQ KGWRNEKTIL 490 500 510 520 530 540 LNEFHTRIPD LPIFVSTLSN SLASNKDNRI LPLSISIIFN YYSKMFPNLF SINLPSSNIY 550 560 570 580 590 600 TDIMQKSKIS GIEFAILDNY LQFQEFNSTQ TRWWNPSSGG NSLFTLLLKL ASSKNASNVI 610 620 630 640 650 660 TTRISNLLDE LTRTNVIFNI SLISPVMALV NSLQGLSLQV SEIDNMEQVW KWFDETISRV 670 680 690 700 710 720 VKTPYKYVDM AKEYNYISPF IMCLSEQWKY VDKSGNPEFL IKWLILFLRN MIFIGEDHIG 730 740 750 760 770 780 IDKLVKNVFP EVSDHDVNIY LKLDSFEENI KKTNSSNSLI SSMKSSSFFQ YISALPSKNL 790 800 810 820 830 840 MNISRLPVNK LDAAGILFRV QLLVEDDSVV YDNWFEATAC ELTGKIASYM VTDTEFPIIK 850 860 870 880 890 900 VLERYINFAL PKLAIEKRNA LLMKKSRFMC NLIGAVCFET GHQLVEFREI IQKVVFSGEN 910 920 930 940 950 960 VEEYANYNEL YQKEDVNAFL TSVSEYLSTS ALTSLLMCST KLESTRNILQ KLFNEGKTIK 970 980 990 1000 1010 1020 ISLVKNILNK AANEDPASIK EVNISLAKFF EENKVCVDAS SDPMGKLSLS ETTSLINSFV 1030 1040 1050 1060 1070 1080 SSDLNYLVLK AFYRWEHFSF PSFIPSIWRI KDSPLLSIVT TAALFKHMQD KDFSAFAHET 1090 1100 1110 1120 1130 1140 ISKYGNEIAK STYTTSKSEI FDEILNMITT YIDFYDETKR NEILKCVLSQ SDHKYHAATV 1150 1160 1170 1180 1190 1200 RYIAAHNNFT YPGVETWLHK TLLYLTKYLS ERKVISNSFF ELLRAMAELL KLEEVPNKLN 1210 1220 1230 1240 1250 1260 VKIINSQLEA ILGSEWIKQI KVLEYVIVLI FCVSKKSIQS QRMVQLLLSN DSYSSIMIKD 1270 1280 1290 1300 1310 1320 NDEDSSYRKF LSTMILFSLF SIDPVVNSTP IVQEKLLTFY SGTISSNDKL ILKILETIES 1330 1340 1350 1360 1370 1380 HTATSWTNMI FSWEFIKDEE EEILEAIGDT RLITKEREGL ILTLQKNMIK KSIDRYVLER 1390 1400 1410 1420 1430 1440 PQVPELYTDS NTNNYDATTR CDLVKKYYDD TERSGVDMYD PLFLLLLIIH NKELVKMVKD 1450 1460 1470 1480 1490 1500 DEGNVTYRYE FENFLDCKIF QFIICSLSDC HTVANISYEH LSNLASSLEK KTAQMNLEKQ 1510 1520 1530 1540 1550 1560 ITSKDNERKE SDSDLIKYNS IYQVLIKRIL YQRQQNQDPI NPLIWFSISR IVDLLGSPTA 1570 1580 1590 1600 1610 1620 PLHEKAYRWV LSNSTIRSWD IPMVSDVMMS YNKRQQDDNK KEIDMEIYYG ELSWVLTTIC 1630 1640 1650 1660 1670 1680 KGIKTDEDYK MLEKKGVFEW LLNLINMPYL KERLRELIYF IFYKVQRVAD DGGLNLISRN 1690 1700 1710 1720 1730 1740 GIVSFFEVLN NNIKSRLPQD DILNNIGTLR NENRGTLNTT LRLAQEQNGI EKLLLGYNEL 1750 1760 VKSQKRLILW TEGDSDNVVK RLRK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequencing and analysis of 51.6 kilobases on the left arm of chromosome XI from Saccharomyces cerevisiae reveals 23 open reading frames including the FAS1 gene." Wiemann S., Voss H., Schwager C., Rupp T., Stegemann J., Zimmermann J., Grothues D., Sensen C., Erfle H., Hewitt N., Banrevi A., Ansorge W. Yeast 9:1343-1348(1993) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Complete DNA sequence of yeast chromosome XI." Dujon B., Alexandraki D., Andre B., Ansorge W., Baladron V., Ballesta J.P.G., Banrevi A., Bolle P.-A., Bolotin-Fukuhara M., Bossier P., Bou G., Boyer J., Buitrago M.J., Cheret G., Colleaux L., Daignan-Fornier B., del Rey F., Dion C. , Domdey H., Duesterhoeft A., Duesterhus S., Entian K.-D., Erfle H., Esteban P.F., Feldmann H., Fernandes L., Fobo G.M., Fritz C., Fukuhara H., Gabel C., Gaillon L., Garcia-Cantalejo J.M., Garcia-Ramirez J.J., Gent M.E., Ghazvini M., Goffeau A., Gonzalez A., Grothues D., Guerreiro P., Hegemann J.H., Hewitt N., Hilger F., Hollenberg C.P., Horaitis O., Indge K.J., Jacquier A., James C.M., Jauniaux J.-C., Jimenez A., Keuchel H., Kirchrath L., Kleine K., Koetter P., Legrain P., Liebl S., Louis E.J., Maia e Silva A., Marck C., Monnier A.-L., Moestl D., Mueller S., Obermaier B., Oliver S.G., Pallier C., Pascolo S., Pfeiffer F., Philippsen P., Planta R.J., Pohl F.M., Pohl T.M., Poehlmann R., Portetelle D., Purnelle B., Puzos V., Ramezani Rad M., Rasmussen S.W., Remacha M.A., Revuelta J.L., Richard G.-F., Rieger M., Rodrigues-Pousada C., Rose M., Rupp T., Santos M.A., Schwager C., Sensen C., Skala J., Soares H., Sor F., Stegemann J., Tettelin H., Thierry A., Tzermia M., Urrestarazu L.A., van Dyck L., van Vliet-Reedijk J.C., Valens M., Vandenbol M., Vilela C., Vissers S., von Wettstein D., Voss H., Wiemann S., Xu G., Zimmermann J., Haasemann M., Becker I., Mewes H.-W. Nature 369:371-378(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[3]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[4]	"Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[5]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[6]	"Exploration of essential gene functions via titratable promoter alleles." Mnaimneh S., Davierwala A.P., Haynes J., Moffat J., Peng W.-T., Zhang W., Yang X., Pootoolal J., Chua G., Lopez A., Trochesset M., Morse D., Krogan N.J., Hiley S.L., Li Z., Morris Q., Grigull J., Mitsakakis N. , Roberts C.J., Greenblatt J.F., Boone C., Kaiser C.A., Andrews B.J., Hughes T.R. Cell 118:31-44(2004) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, INTERACTION WITH URB2.
[7]	"Npa1p, a component of very early pre-60S ribosomal particles, associates with a subset of small nucleolar RNPs required for peptidyl transferase center modification." Dez C., Froment C., Noaillac-Depeyre J., Monsarrat B., Caizergues-Ferrer M., Henry Y. Mol. Cell. Biol. 24:6324-6337(2004) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, SUBUNIT, INTERACTION WITH URB2, SUBCELLULAR LOCATION.
[8]	"Npa1p is an essential trans-acting factor required for an early step in the assembly of 60S ribosomal subunits in Saccharomyces cerevisiae." Rosado I.V., de la Cruz J. RNA 10:1073-1083(2004) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, ASSOCIATION WITH PRE-RIBOSOMAL PARTICLES, SUBCELLULAR LOCATION.
[9]	"Characterization of Saccharomyces cerevisiae Npa2p (Urb2p) reveals a Low-molecular-mass complex containing Dbp6p, Npa1p (Urb1p), Nop8p, and Rsa3p involved in early steps of 60S ribosomal subunit biogenesis." Rosado I.V., Dez C., Lebaron S., Caizergues-Ferrer M., Henry Y., de la Cruz J. Mol. Cell. Biol. 27:1207-1221(2007) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION IN A COMPLEX WITH DBP6; NOP8; URB2 AND RSA3.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X74152 Genomic DNA. Translation: CAA52264.1. Z28014 Genomic DNA. Translation: CAA81849.1. BK006944 Genomic DNA. Translation: DAA09142.1.
PIR	S37827.
RefSeq	NP_012911.3. NM_001179580.3.
3D structure databases
ProteinModelPortal	P34241.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-2701N.
IntAct	P34241. 19 interactions.
MINT	MINT-668747.
STRING	4932.YKL014C.
Proteomic databases
PaxDb	P34241.
PeptideAtlas	P34241.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YKL014C; YKL014C; YKL014C.
GeneID	853855.
KEGG	sce:YKL012W. sce:YKL014C.
Organism-specific databases
CYGD	YKL014c.
SGD	S000001497. URB1.
Phylogenomic databases
eggNOG	NOG324825.
HOGENOM	HOG000141826.
KO	K12821. K14861.
OMA	LVAPYMN.
OrthoDB	EOG4NPBBM.
Gene expression databases
Genevestigator	P34241.
GermOnline	YKL014C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR021714. Npa1_N. [Graphical view]
Pfam	PF11707. Npa1. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	975094.

Entry information

Entry name

URB1_YEAST

Accession

Primary (citable) accession number: P34241
Secondary accession number(s): D6VXS2, P34242

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1994
Last sequence update:	June 1, 1994
Last modified:	April 3, 2013
This is version 84 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program