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P34241

- URB1_YEAST

UniProt

P34241 - URB1_YEAST

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Protein

Nucleolar pre-ribosomal-associated protein 1

Gene

URB1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli

Functioni

Required for 60S ribosomal subunit formation and pre-rRNA processing. Required for normal accumulation of 25S and 5.8S rRNAs.3 Publications

GO - Biological processi

  1. maturation of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) Source: SGD
  2. maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) Source: SGD
Complete GO annotation...

Keywords - Biological processi

Ribosome biogenesis, rRNA processing

Enzyme and pathway databases

BioCyciYEAST:G3O-31823-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Nucleolar pre-ribosomal-associated protein 1
Alternative name(s):
Unhealthy ribosome biogenesis protein 1
Gene namesi
Name:URB1
Synonyms:NPA1
Ordered Locus Names:YKL014C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome XI

Organism-specific databases

CYGDiYKL014c.
SGDiS000001497. URB1.

Subcellular locationi

Nucleusnucleolus 3 Publications
Note: Accumulates in the immediate vicinity of the dense fibrillar component of the nucleolus.

GO - Cellular componenti

  1. nucleolus Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 17641764Nucleolar pre-ribosomal-associated protein 1PRO_0000203190Add
BLAST

Proteomic databases

MaxQBiP34241.
PaxDbiP34241.
PeptideAtlasiP34241.

Expressioni

Gene expression databases

GenevestigatoriP34241.

Interactioni

Subunit structurei

Associates with pre-60S ribosomal particles. Predominantly associated with the 27SA2 pre-rRNA. Can associate with a subset of box H/ACA and box C/D small nucleolar RNPs (snoRNPs) required for peptidyl transferase center modification and with small RNAs snR37 and snR42. Interacts with URB2. Together with DBP6, NOP8, URB2 and RSA3, forms an RNA-independent complex, which is required during early maturation of nascent 60S ribosomal subunits.3 Publications

Protein-protein interaction databases

BioGridi34118. 99 interactions.
DIPiDIP-2701N.
IntActiP34241. 17 interactions.
MINTiMINT-668747.
STRINGi4932.YKL014C.

Structurei

3D structure databases

ProteinModelPortaliP34241.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Phylogenomic databases

eggNOGiNOG324825.
HOGENOMiHOG000141826.
InParanoidiP34241.
KOiK14861.
OMAiPYKYVDM.
OrthoDBiEOG76DV31.

Family and domain databases

InterProiIPR021714. Npa1_N.
[Graphical view]
PfamiPF11707. Npa1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P34241-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSNHSEAYGS RDQRREKYTQ GKEFEDGTLE TLESIISAVE DETLSKDYQP
60 70 80 90 100
LIVFFQRGFG AQLVQTWSYY AQVNNHGKFS KTTSLLTKTL RVLSSDTSTV
110 120 130 140 150
TIGSGLIRLI LTDYTKVLYR GLNNMRAQLT NPILRLLKQI VNFNNGQHIE
160 170 180 190 200
ELVSYFDFSL PILPRLLVPS KSELANGNSS ADSSKHDSLR FTFIKFWLTL
210 220 230 240 250
ISNASPFVRK ELLTENFKIM SNLFKFMNKA DSDKLSEHIL SVFINDILKE
260 270 280 290 300
KSFKRTTKTK ILNELAASKI HHFYYSSNKN LVKKANEFFL TFGASRDFSV
310 320 330 340 350
AFPDNCVWFK NSVADGASHG APITVNQVEF QIHNKLLFNT LRLFKPWEDT
360 370 380 390 400
LQLGTLIKIL ENVPELVAPY SIFLTTNGNY DPKMTSYWFG ITLLINKIIN
410 420 430 440 450
LKIPQFMEKV DSNIPPATSL VIENILPSLL TKSSLVKSLQ FETPIIRQLA
460 470 480 490 500
CQSIVLALKK LEKVSTFYDQ KGWRNEKTIL LNEFHTRIPD LPIFVSTLSN
510 520 530 540 550
SLASNKDNRI LPLSISIIFN YYSKMFPNLF SINLPSSNIY TDIMQKSKIS
560 570 580 590 600
GIEFAILDNY LQFQEFNSTQ TRWWNPSSGG NSLFTLLLKL ASSKNASNVI
610 620 630 640 650
TTRISNLLDE LTRTNVIFNI SLISPVMALV NSLQGLSLQV SEIDNMEQVW
660 670 680 690 700
KWFDETISRV VKTPYKYVDM AKEYNYISPF IMCLSEQWKY VDKSGNPEFL
710 720 730 740 750
IKWLILFLRN MIFIGEDHIG IDKLVKNVFP EVSDHDVNIY LKLDSFEENI
760 770 780 790 800
KKTNSSNSLI SSMKSSSFFQ YISALPSKNL MNISRLPVNK LDAAGILFRV
810 820 830 840 850
QLLVEDDSVV YDNWFEATAC ELTGKIASYM VTDTEFPIIK VLERYINFAL
860 870 880 890 900
PKLAIEKRNA LLMKKSRFMC NLIGAVCFET GHQLVEFREI IQKVVFSGEN
910 920 930 940 950
VEEYANYNEL YQKEDVNAFL TSVSEYLSTS ALTSLLMCST KLESTRNILQ
960 970 980 990 1000
KLFNEGKTIK ISLVKNILNK AANEDPASIK EVNISLAKFF EENKVCVDAS
1010 1020 1030 1040 1050
SDPMGKLSLS ETTSLINSFV SSDLNYLVLK AFYRWEHFSF PSFIPSIWRI
1060 1070 1080 1090 1100
KDSPLLSIVT TAALFKHMQD KDFSAFAHET ISKYGNEIAK STYTTSKSEI
1110 1120 1130 1140 1150
FDEILNMITT YIDFYDETKR NEILKCVLSQ SDHKYHAATV RYIAAHNNFT
1160 1170 1180 1190 1200
YPGVETWLHK TLLYLTKYLS ERKVISNSFF ELLRAMAELL KLEEVPNKLN
1210 1220 1230 1240 1250
VKIINSQLEA ILGSEWIKQI KVLEYVIVLI FCVSKKSIQS QRMVQLLLSN
1260 1270 1280 1290 1300
DSYSSIMIKD NDEDSSYRKF LSTMILFSLF SIDPVVNSTP IVQEKLLTFY
1310 1320 1330 1340 1350
SGTISSNDKL ILKILETIES HTATSWTNMI FSWEFIKDEE EEILEAIGDT
1360 1370 1380 1390 1400
RLITKEREGL ILTLQKNMIK KSIDRYVLER PQVPELYTDS NTNNYDATTR
1410 1420 1430 1440 1450
CDLVKKYYDD TERSGVDMYD PLFLLLLIIH NKELVKMVKD DEGNVTYRYE
1460 1470 1480 1490 1500
FENFLDCKIF QFIICSLSDC HTVANISYEH LSNLASSLEK KTAQMNLEKQ
1510 1520 1530 1540 1550
ITSKDNERKE SDSDLIKYNS IYQVLIKRIL YQRQQNQDPI NPLIWFSISR
1560 1570 1580 1590 1600
IVDLLGSPTA PLHEKAYRWV LSNSTIRSWD IPMVSDVMMS YNKRQQDDNK
1610 1620 1630 1640 1650
KEIDMEIYYG ELSWVLTTIC KGIKTDEDYK MLEKKGVFEW LLNLINMPYL
1660 1670 1680 1690 1700
KERLRELIYF IFYKVQRVAD DGGLNLISRN GIVSFFEVLN NNIKSRLPQD
1710 1720 1730 1740 1750
DILNNIGTLR NENRGTLNTT LRLAQEQNGI EKLLLGYNEL VKSQKRLILW
1760
TEGDSDNVVK RLRK
Length:1,764
Mass (Da):203,287
Last modified:June 1, 1994 - v2
Checksum:iD4EC1FA19E051367
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X74152 Genomic DNA. Translation: CAA52264.1.
Z28014 Genomic DNA. Translation: CAA81849.1.
BK006944 Genomic DNA. Translation: DAA09142.1.
PIRiS37827.
RefSeqiNP_012911.3. NM_001179580.3.

Genome annotation databases

EnsemblFungiiYKL014C; YKL014C; YKL014C.
GeneIDi853855.
KEGGisce:YKL014C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X74152 Genomic DNA. Translation: CAA52264.1 .
Z28014 Genomic DNA. Translation: CAA81849.1 .
BK006944 Genomic DNA. Translation: DAA09142.1 .
PIRi S37827.
RefSeqi NP_012911.3. NM_001179580.3.

3D structure databases

ProteinModelPortali P34241.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 34118. 99 interactions.
DIPi DIP-2701N.
IntActi P34241. 17 interactions.
MINTi MINT-668747.
STRINGi 4932.YKL014C.

Proteomic databases

MaxQBi P34241.
PaxDbi P34241.
PeptideAtlasi P34241.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YKL014C ; YKL014C ; YKL014C .
GeneIDi 853855.
KEGGi sce:YKL014C.

Organism-specific databases

CYGDi YKL014c.
SGDi S000001497. URB1.

Phylogenomic databases

eggNOGi NOG324825.
HOGENOMi HOG000141826.
InParanoidi P34241.
KOi K14861.
OMAi PYKYVDM.
OrthoDBi EOG76DV31.

Enzyme and pathway databases

BioCyci YEAST:G3O-31823-MONOMER.

Miscellaneous databases

NextBioi 975094.

Gene expression databases

Genevestigatori P34241.

Family and domain databases

InterProi IPR021714. Npa1_N.
[Graphical view ]
Pfami PF11707. Npa1. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Sequencing and analysis of 51.6 kilobases on the left arm of chromosome XI from Saccharomyces cerevisiae reveals 23 open reading frames including the FAS1 gene."
    Wiemann S., Voss H., Schwager C., Rupp T., Stegemann J., Zimmermann J., Grothues D., Sensen C., Erfle H., Hewitt N., Banrevi A., Ansorge W.
    Yeast 9:1343-1348(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "Complete DNA sequence of yeast chromosome XI."
    Dujon B., Alexandraki D., Andre B., Ansorge W., Baladron V., Ballesta J.P.G., Banrevi A., Bolle P.-A., Bolotin-Fukuhara M., Bossier P., Bou G., Boyer J., Buitrago M.J., Cheret G., Colleaux L., Daignan-Fornier B., del Rey F., Dion C.
    , Domdey H., Duesterhoeft A., Duesterhus S., Entian K.-D., Erfle H., Esteban P.F., Feldmann H., Fernandes L., Fobo G.M., Fritz C., Fukuhara H., Gabel C., Gaillon L., Garcia-Cantalejo J.M., Garcia-Ramirez J.J., Gent M.E., Ghazvini M., Goffeau A., Gonzalez A., Grothues D., Guerreiro P., Hegemann J.H., Hewitt N., Hilger F., Hollenberg C.P., Horaitis O., Indge K.J., Jacquier A., James C.M., Jauniaux J.-C., Jimenez A., Keuchel H., Kirchrath L., Kleine K., Koetter P., Legrain P., Liebl S., Louis E.J., Maia e Silva A., Marck C., Monnier A.-L., Moestl D., Mueller S., Obermaier B., Oliver S.G., Pallier C., Pascolo S., Pfeiffer F., Philippsen P., Planta R.J., Pohl F.M., Pohl T.M., Poehlmann R., Portetelle D., Purnelle B., Puzos V., Ramezani Rad M., Rasmussen S.W., Remacha M.A., Revuelta J.L., Richard G.-F., Rieger M., Rodrigues-Pousada C., Rose M., Rupp T., Santos M.A., Schwager C., Sensen C., Skala J., Soares H., Sor F., Stegemann J., Tettelin H., Thierry A., Tzermia M., Urrestarazu L.A., van Dyck L., van Vliet-Reedijk J.C., Valens M., Vandenbol M., Vilela C., Vissers S., von Wettstein D., Voss H., Wiemann S., Xu G., Zimmermann J., Haasemann M., Becker I., Mewes H.-W.
    Nature 369:371-378(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  4. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
  5. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  6. Cited for: FUNCTION, INTERACTION WITH URB2.
  7. "Npa1p, a component of very early pre-60S ribosomal particles, associates with a subset of small nucleolar RNPs required for peptidyl transferase center modification."
    Dez C., Froment C., Noaillac-Depeyre J., Monsarrat B., Caizergues-Ferrer M., Henry Y.
    Mol. Cell. Biol. 24:6324-6337(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBUNIT, INTERACTION WITH URB2, SUBCELLULAR LOCATION.
  8. "Npa1p is an essential trans-acting factor required for an early step in the assembly of 60S ribosomal subunits in Saccharomyces cerevisiae."
    Rosado I.V., de la Cruz J.
    RNA 10:1073-1083(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, ASSOCIATION WITH PRE-RIBOSOMAL PARTICLES, SUBCELLULAR LOCATION.
  9. "Characterization of Saccharomyces cerevisiae Npa2p (Urb2p) reveals a Low-molecular-mass complex containing Dbp6p, Npa1p (Urb1p), Nop8p, and Rsa3p involved in early steps of 60S ribosomal subunit biogenesis."
    Rosado I.V., Dez C., Lebaron S., Caizergues-Ferrer M., Henry Y., de la Cruz J.
    Mol. Cell. Biol. 27:1207-1221(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION IN A COMPLEX WITH DBP6; NOP8; URB2 AND RSA3.

Entry informationi

Entry nameiURB1_YEAST
AccessioniPrimary (citable) accession number: P34241
Secondary accession number(s): D6VXS2, P34242
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: June 1, 1994
Last modified: October 29, 2014
This is version 93 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 5890 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  2. Yeast chromosome XI
    Yeast (Saccharomyces cerevisiae) chromosome XI: entries and gene names

External Data

Dasty 3