Reviewed, UniProtKB/Swiss-Prot P34240 (ZRT3_YEAST)

Names and origin

Protein names

Recommended name:
    Zinc-regulated transporter 3
Alternative name(s):
    Vacuolar membrane zinc transporter

Gene names

Name:	ZRT3
Ordered Locus Names:	YKL175W
ORF Names:	YKL640

Organism

Saccharomyces cerevisiae (Baker's yeast) [Complete proteome]

Taxonomic identifier

4932 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	503 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Transports zinc from storage in the vacuole to the cytoplasm. Ref.4
Subcellular location	Vacuole membrane; Multi-pass membrane protein Ref.4.
Sequence similarities	Belongs to the ZIP transporter (TC 2.A.5) family. [View classification]

Ontologies

Keywords
Biological process	Ion transport Transport Zinc transport
Cellular component	Membrane Vacuole
Domain	Transmembrane
Ligand	Zinc
PTM	Phosphoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cellular zinc ion homeostasis Ref.4 Inferred from mutant phenotype. Source: SGD transmembrane transport Inferred from electronic annotation. Source: InterPro zinc ion transport Ref.4 Inferred from mutant phenotype. Source: SGD
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell vacuolar membrane Ref.4 Inferred from direct assay. Source: SGD
Molecular function	zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW zinc ion transmembrane transporter activity Ref.4 Inferred from mutant phenotype. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 503	503	Zinc-regulated transporter 3		PRO_0000203140
Regions
	Transmembrane	8 – 28	21	Potential
	Transmembrane	42 – 62	21	Potential
	Transmembrane	75 – 95	21	Potential
	Transmembrane	336 – 356	21	Potential
	Transmembrane	371 – 391	21	Potential
	Transmembrane	398 – 418	21	Potential
	Transmembrane	438 – 458	21	Potential
	Transmembrane	482 – 502	21	Potential
	Compositional bias	102 – 139	38	His-rich
	Compositional bias	153 – 158	6	Poly-Ser
	Compositional bias	322 – 325	4	Poly-His
	Compositional bias	464 – 467	4	Poly-His
Amino acid modifications
	Modified residue	136	1	Phosphoserine Ref.7
	Modified residue	153	1	Phosphoserine Ref.7
	Modified residue	178	1	Phosphoserine Ref.6
	Modified residue	188	1	Phosphoserine Ref.7
	Modified residue	262	1	Phosphoserine Ref.7 Ref.5

Sequences

	Sequence		Length	Mass (Da)
	P34240-1 [UniParc]. Last modified June 1, 1994. Version 2. Checksum: DBAD8CE2C2809082	FASTA	503	55,403
	10 20 30 40 50 60 MEKIPRWLLF SLISSVLCIL GALCVPLLSV AFDSKRNSQS KLVNYGLSLS AGSMITTSLY 70 80 90 100 110 120 MLLPRIEKSN RFKVFPGLLL GICLSFFLNY LVHAFASESL VHCADSGDHA TGSHIHSKSH 130 140 150 160 170 180 SHSHSHSHAD SHSNFSNDHD LENAPSEHGY ATSSSSVSEN DPLITKDSDR PQMKKKMSLI 190 200 210 220 230 240 DLLTRRKSEG ECCDLNKCTP LLQSEQPEYI ACVPPVIKSS QSERNVPHGC EGSEDNGQSD 250 260 270 280 290 300 DKDHRGLVCV ENNIGYDLEN LSLYRKNFLS SRHHHSSESP ENYGSNQLSH SFSSPLGNDV 310 320 330 340 350 360 TENPAALADT QYHPENGSLY PHHHHLETPF SKLLSIGMQT CLVLALHKFP EGFIIFYTNR 370 380 390 400 410 420 SDSSKSLGFS IFLSLTIHNF VEGFAMTLPF YTVFESKWVA ILITAVLGGG SQPLGALIGY 430 440 450 460 470 480 FIFKGSTPRD HEPNMDFLLS VTAGFLLVIG LQMFQTGIGF SDGHHHHQGE GDEEMKQSHS 490 500 SGTTCLKWCC TGVLLILASA LFT

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequencing and analysis of a 20.5 kb DNA segment located on the left arm of yeast chromosome XI." Vandenbol M., Bolle P.-A., Dion C., Portetelle D., Hilger F. Yeast 10:S25-S33(1994) [PubMed: 8091858] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[2]	"Complete DNA sequence of yeast chromosome XI." Dujon B., Alexandraki D., Andre B., Ansorge W., Baladron V., Ballesta J.P.G., Banrevi A., Bolle P.-A., Bolotin-Fukuhara M., Bossier P., Bou G., Boyer J., Buitrago M.J., Cheret G., Colleaux L., Daignan-Fornier B., del Rey F., Dion C. , Domdey H., Duesterhoeft A., Duesterhus S., Entian K.-D., Erfle H., Esteban P.F., Feldmann H., Fernandes L., Fobo G.M., Fritz C., Fukuhara H., Gabel C., Gaillon L., Garcia-Cantalejo J.M., Garcia-Ramirez J.J., Gent M.E., Ghazvini M., Goffeau A., Gonzalez A., Grothues D., Guerreiro P., Hegemann J.H., Hewitt N., Hilger F., Hollenberg C.P., Horaitis O., Indge K.J., Jacquier A., James C.M., Jauniaux J.-C., Jimenez A., Keuchel H., Kirchrath L., Kleine K., Koetter P., Legrain P., Liebl S., Louis E.J., Maia e Silva A., Marck C., Monnier A.-L., Moestl D., Mueller S., Obermaier B., Oliver S.G., Pallier C., Pascolo S., Pfeiffer F., Philippsen P., Planta R.J., Pohl F.M., Pohl T.M., Poehlmann R., Portetelle D., Purnelle B., Puzos V., Ramezani Rad M., Rasmussen S.W., Remacha M.A., Revuelta J.L., Richard G.-F., Rieger M., Rodrigues-Pousada C., Rose M., Rupp T., Santos M.A., Schwager C., Sensen C., Skala J., Soares H., Sor F., Stegemann J., Tettelin H., Thierry A., Tzermia M., Urrestarazu L.A., van Dyck L., van Vliet-Reedijk J.C., Valens M., Vandenbol M., Vilela C., Vissers S., von Wettstein D., Voss H., Wiemann S., Xu G., Zimmermann J., Haasemann M., Becker I., Mewes H.-W. Nature 369:371-378(1994) [PubMed: 8196765] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[3]	"Sequencing and analysis of 51.6 kilobases on the left arm of chromosome XI from Saccharomyces cerevisiae reveals 23 open reading frames including the FAS1 gene." Wiemann S., Voss H., Schwager C., Rupp T., Stegemann J., Zimmermann J., Grothues D., Sensen C., Erfle H., Hewitt N., Banrevi A., Ansorge W. Yeast 9:1343-1348(1993) [PubMed: 8154185] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-233.
[4]	"Zinc transporters that regulate vacuolar zinc storage in Saccharomyces cerevisiae." MacDiarmid C.W., Gaither L.A., Eide D. EMBO J. 19:2845-2855(2000) [PubMed: 10856230] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION.
[5]	"Quantitative phosphoproteomics applied to the yeast pheromone signaling pathway." Gruhler A., Olsen J.V., Mohammed S., Mortensen P., Faergeman N.J., Mann M., Jensen O.N. Mol. Cell. Proteomics 4:310-327(2005) [PubMed: 15665377] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-262, MASS SPECTROMETRY.
[6]	"Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae." Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P. J. Proteome Res. 6:1190-1197(2007) [PubMed: 17330950] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-178, MASS SPECTROMETRY.
[7]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed: 18407956] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-136; SER-153; SER-188 AND SER-262, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Z26878 Genomic DNA. Translation: CAA81511.1. X74151 Genomic DNA. Translation: CAA52263.1. Z28175 Genomic DNA. Translation: CAA82017.1.
PIR	S38005.
RefSeq	NP_012746.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-5610N.
IntAct	P34240. 3 interactions.
STRING	P34240.
Proteomic databases
PeptideAtlas	P34240.
Genome annotation databases
Ensembl	YKL175W; YKL175W; YKL175W; Saccharomyces cerevisiae. [Genome view]
GeneID	853679.
KEGG	sce:YKL175W.
NMPDR	fig|4932.3.peg.3724.
Organism-specific databases
CYGD	YKL175w.
SGD	S000001658. ZRT3.
Phylogenomic databases
eggNOG	fuNOG07022.
HOGENOM	HBG202404.
OMA	FTEGFSM.
OrthoDB	EOG9J9PG1.
PhylomeDB	P34240.
Gene expression databases
ArrayExpress	P34240.
Genevestigator	P34240.
GermOnline	YKL175W. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR003689. ZIP. [Graphical view]
Pfam	PF02535. Zip. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	974637.

Entry information

Entry name

ZRT3_YEAST

Accession

Primary (citable) accession number: P34240

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1994
Last sequence update:	June 1, 1994
Last modified:	January 19, 2010
This is version 71 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)