P34240 (ZRT3_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Zinc-regulated transporter 3

Alternative name(s):
Vacuolar membrane zinc transporter

Gene names

Name:	ZRT3
Ordered Locus Names:	YKL175W
ORF Names:	YKL640

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › Saccharomyces cerevisiae

Protein attributes

Sequence length	503 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Transports zinc from storage in the vacuole to the cytoplasm. Ref.5
Subcellular location	Vacuole membrane; Multi-pass membrane protein Ref.5.
Sequence similarities	Belongs to the ZIP transporter (TC 2.A.5) family. [View classification]

Ontologies

Keywords
Biological process	Ion transport Transport Zinc transport
Cellular component	Membrane Vacuole
Domain	Transmembrane Transmembrane helix
Ligand	Zinc
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cellular zinc ion homeostasis Inferred from mutant phenotype Ref.5. Source: SGD
Cellular_component	fungal-type vacuole membrane Inferred from direct assay Ref.5. Source: SGD integral to membrane Inferred from sequence model PubMed 12192589. Source: SGD
Molecular_function	zinc ion transmembrane transporter activity Inferred from mutant phenotype Ref.5. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 503	503	Zinc-regulated transporter 3		PRO_0000203140
Regions
	Transmembrane	8 – 28	21	Helical; Potential
	Transmembrane	42 – 62	21	Helical; Potential
	Transmembrane	75 – 95	21	Helical; Potential
	Transmembrane	336 – 356	21	Helical; Potential
	Transmembrane	371 – 391	21	Helical; Potential
	Transmembrane	398 – 418	21	Helical; Potential
	Transmembrane	438 – 458	21	Helical; Potential
	Transmembrane	482 – 502	21	Helical; Potential
	Compositional bias	102 – 139	38	His-rich
	Compositional bias	153 – 158	6	Poly-Ser
	Compositional bias	322 – 325	4	Poly-His
	Compositional bias	464 – 467	4	Poly-His
Amino acid modifications
	Modified residue	136	1	Phosphoserine Ref.8
	Modified residue	153	1	Phosphoserine Ref.8
	Modified residue	178	1	Phosphoserine Ref.7
	Modified residue	188	1	Phosphoserine Ref.8
	Modified residue	262	1	Phosphoserine Ref.6 Ref.8

Sequences

	Sequence		Length	Mass (Da)
	P34240 [UniParc]. Last modified June 1, 1994. Version 2. Checksum: DBAD8CE2C2809082	FASTA	503	55,403
	10 20 30 40 50 60 MEKIPRWLLF SLISSVLCIL GALCVPLLSV AFDSKRNSQS KLVNYGLSLS AGSMITTSLY 70 80 90 100 110 120 MLLPRIEKSN RFKVFPGLLL GICLSFFLNY LVHAFASESL VHCADSGDHA TGSHIHSKSH 130 140 150 160 170 180 SHSHSHSHAD SHSNFSNDHD LENAPSEHGY ATSSSSVSEN DPLITKDSDR PQMKKKMSLI 190 200 210 220 230 240 DLLTRRKSEG ECCDLNKCTP LLQSEQPEYI ACVPPVIKSS QSERNVPHGC EGSEDNGQSD 250 260 270 280 290 300 DKDHRGLVCV ENNIGYDLEN LSLYRKNFLS SRHHHSSESP ENYGSNQLSH SFSSPLGNDV 310 320 330 340 350 360 TENPAALADT QYHPENGSLY PHHHHLETPF SKLLSIGMQT CLVLALHKFP EGFIIFYTNR 370 380 390 400 410 420 SDSSKSLGFS IFLSLTIHNF VEGFAMTLPF YTVFESKWVA ILITAVLGGG SQPLGALIGY 430 440 450 460 470 480 FIFKGSTPRD HEPNMDFLLS VTAGFLLVIG LQMFQTGIGF SDGHHHHQGE GDEEMKQSHS 490 500 SGTTCLKWCC TGVLLILASA LFT

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequencing and analysis of a 20.5 kb DNA segment located on the left arm of yeast chromosome XI." Vandenbol M., Bolle P.-A., Dion C., Portetelle D., Hilger F. Yeast 10:S25-S33(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[2]	"Complete DNA sequence of yeast chromosome XI." Dujon B., Alexandraki D., Andre B., Ansorge W., Baladron V., Ballesta J.P.G., Banrevi A., Bolle P.-A., Bolotin-Fukuhara M., Bossier P., Bou G., Boyer J., Buitrago M.J., Cheret G., Colleaux L., Daignan-Fornier B., del Rey F., Dion C. , Domdey H., Duesterhoeft A., Duesterhus S., Entian K.-D., Erfle H., Esteban P.F., Feldmann H., Fernandes L., Fobo G.M., Fritz C., Fukuhara H., Gabel C., Gaillon L., Garcia-Cantalejo J.M., Garcia-Ramirez J.J., Gent M.E., Ghazvini M., Goffeau A., Gonzalez A., Grothues D., Guerreiro P., Hegemann J.H., Hewitt N., Hilger F., Hollenberg C.P., Horaitis O., Indge K.J., Jacquier A., James C.M., Jauniaux J.-C., Jimenez A., Keuchel H., Kirchrath L., Kleine K., Koetter P., Legrain P., Liebl S., Louis E.J., Maia e Silva A., Marck C., Monnier A.-L., Moestl D., Mueller S., Obermaier B., Oliver S.G., Pallier C., Pascolo S., Pfeiffer F., Philippsen P., Planta R.J., Pohl F.M., Pohl T.M., Poehlmann R., Portetelle D., Purnelle B., Puzos V., Ramezani Rad M., Rasmussen S.W., Remacha M.A., Revuelta J.L., Richard G.-F., Rieger M., Rodrigues-Pousada C., Rose M., Rupp T., Santos M.A., Schwager C., Sensen C., Skala J., Soares H., Sor F., Stegemann J., Tettelin H., Thierry A., Tzermia M., Urrestarazu L.A., van Dyck L., van Vliet-Reedijk J.C., Valens M., Vandenbol M., Vilela C., Vissers S., von Wettstein D., Voss H., Wiemann S., Xu G., Zimmermann J., Haasemann M., Becker I., Mewes H.-W. Nature 369:371-378(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[3]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[4]	"Sequencing and analysis of 51.6 kilobases on the left arm of chromosome XI from Saccharomyces cerevisiae reveals 23 open reading frames including the FAS1 gene." Wiemann S., Voss H., Schwager C., Rupp T., Stegemann J., Zimmermann J., Grothues D., Sensen C., Erfle H., Hewitt N., Banrevi A., Ansorge W. Yeast 9:1343-1348(1993) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-233.
[5]	"Zinc transporters that regulate vacuolar zinc storage in Saccharomyces cerevisiae." MacDiarmid C.W., Gaither L.A., Eide D. EMBO J. 19:2845-2855(2000) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION.
[6]	"Quantitative phosphoproteomics applied to the yeast pheromone signaling pathway." Gruhler A., Olsen J.V., Mohammed S., Mortensen P., Faergeman N.J., Mann M., Jensen O.N. Mol. Cell. Proteomics 4:310-327(2005) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-262, MASS SPECTROMETRY. Strain: YAL6B.
[7]	"Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae." Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P. J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-178, MASS SPECTROMETRY. Strain: ADR376.
[8]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-136; SER-153; SER-188 AND SER-262, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Z26878 Genomic DNA. Translation: CAA81511.1. X74151 Genomic DNA. Translation: CAA52263.1. Z28175 Genomic DNA. Translation: CAA82017.1. BK006944 Genomic DNA. Translation: DAA08991.1.
PIR	S38005.
RefSeq	NP_012746.1. NM_001179741.1.
3D structure databases
ProteinModelPortal	P34240.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-5610N.
MINT	MINT-528697.
STRING	4932.YKL175W.
Proteomic databases
PaxDb	P34240.
PeptideAtlas	P34240.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YKL175W; YKL175W; YKL175W.
GeneID	853679.
KEGG	sce:YKL175W.
Organism-specific databases
CYGD	YKL175w.
SGD	S000001658. ZRT3.
Phylogenomic databases
eggNOG	COG0428.
HOGENOM	HOG000142066.
KO	K07238.
OMA	FTEGFSM.
OrthoDB	EOG4SN4XV.
Enzyme and pathway databases
BioCyc	YEAST:G3O-31942-MONOMER.
Gene expression databases
Genevestigator	P34240.
GermOnline	YKL175W. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR003689. ZIP. [Graphical view]
Pfam	PF02535. Zip. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	974637.

Entry information

Entry name

ZRT3_YEAST

Accession

Primary (citable) accession number: P34240
Secondary accession number(s): D6VX25

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1994
Last sequence update:	June 1, 1994
Last modified:	May 29, 2013
This is version 92 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program