P34239 (LST4_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Protein LST4

Alternative name(s):
Lethal with SEC30 protein 4

Gene names

Name:	LST4
Ordered Locus Names:	YKL176C
ORF Names:	YKL642

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	828 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Involved in extracellular amino acid uptake. Required for the trafficking of the GAP1 nitrogen-regulated general amino acid permease from the Golgi to the plasma membrane. Ref.5 Ref.6 Ref.7
Sequence similarities	Belongs to the LST4 family.

Ontologies

Keywords
Biological process	Amino-acid transport Protein transport Transport
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	Golgi to plasma membrane transport Inferred from direct assay Ref.5. Source: SGD amino acid transport Inferred from electronic annotation. Source: UniProtKB-KW intracellular protein transport Inferred from direct assay Ref.5. Source: SGD
Cellular component	vesicle coat Inferred from direct assay Ref.5. Source: SGD
Molecular function	protein transporter activity Inferred from direct assay Ref.5. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 828	828	Protein LST4		PRO_0000203139
Regions
	Compositional bias	437 – 570	134	Ser-rich
Amino acid modifications
	Modified residue	418	1	Phosphoserine Ref.8
	Modified residue	547	1	Phosphoserine Ref.9

Sequences

	Sequence		Length	Mass (Da)
	P34239 [UniParc]. Last modified February 1, 1994. Version 1. Checksum: 4CE2DEA23B0A18F8	FASTA	828	93,359
	10 20 30 40 50 60 MLGNLLRNKT SSSGFEKSSE HSDFSSVVPN VPVYCKAAST GTTKTAAGAL LDTAVNVEKP 70 80 90 100 110 120 SEMLSTTSPP ILDHISDDLK LKLFGSRDIP YSRPIDTLQN NGGLGTDKIT SINEKTYAFR 130 140 150 160 170 180 ILIIEEAGQM ACRNNYRDIF DYTTSKISNS MEQIRPSELK EYIFGSPVRS SDLTQCDKIR 190 200 210 220 230 240 TIPNSDLVLI TRIFYYTHQY NRIAISLCIP RILLPVVAES WSSISSWLTQ TQKMLIGFLT 250 260 270 280 290 300 KNRIMQENTG NYSNNSVIKL SNIDIRTHYP KEIEIMVQTL QKRVIPGLRS MSEIPRLFLY 310 320 330 340 350 360 PETFKEFVHV WFKSIFNWIE IKDGPKLGFL PLLMAMIISD YRHTIRELKT SKIVILSGNM 370 380 390 400 410 420 VVANKLLFIL SALLEPKYKG QITIRRENIR SDSSAVSRNK SNNNFVDKPE TELSTLTSTD 430 440 450 460 470 480 NLLSRTENNS NHNYNNSNVS SNSIGSPNFH SLRKGWQIPN RRNSNTSVSV SSSESLAEVI 490 500 510 520 530 540 QPSSFKSGSS SLHYLSSSIS SQPGSYGSWF NKRPTISQFF QPSPSLKHNE SWERLQTTAG 550 560 570 580 590 600 NMQRTSSSSS LQQATSRLSL TTPQQSPSIS EYDEYPWMGT PGSPNVGDVS HAPPLVKNIS 610 620 630 640 650 660 YKFPLKNVEL KRDCQRISQD DLLDEAFERI CQPSLADLNS TYEIFPGNSS YADILTTDSD 670 680 690 700 710 720 IDDGLMNKPL ELLPKYTMYL THFNNFFQLQ ACPAGQESES RITNSMKIDL LKADYTRSLL 730 740 750 760 770 780 VSLRSRDIRD VALKREFTGN NNNNSNQNIY DENFVGKRKY VLKQKTRKIF SCGKIGKLST 790 800 810 820 SLENCVNFVE NSIKSAMMLY DDNGIDSELR DSEALRIFSS LVHYCNAG

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequencing and analysis of 51.6 kilobases on the left arm of chromosome XI from Saccharomyces cerevisiae reveals 23 open reading frames including the FAS1 gene." Wiemann S., Voss H., Schwager C., Rupp T., Stegemann J., Zimmermann J., Grothues D., Sensen C., Erfle H., Hewitt N., Banrevi A., Ansorge W. Yeast 9:1343-1348(1993) [PubMed: 8154185] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Complete DNA sequence of yeast chromosome XI." Dujon B., Alexandraki D., Andre B., Ansorge W., Baladron V., Ballesta J.P.G., Banrevi A., Bolle P.-A., Bolotin-Fukuhara M., Bossier P., Bou G., Boyer J., Buitrago M.J., Cheret G., Colleaux L., Daignan-Fornier B., del Rey F., Dion C. , Domdey H., Duesterhoeft A., Duesterhus S., Entian K.-D., Erfle H., Esteban P.F., Feldmann H., Fernandes L., Fobo G.M., Fritz C., Fukuhara H., Gabel C., Gaillon L., Garcia-Cantalejo J.M., Garcia-Ramirez J.J., Gent M.E., Ghazvini M., Goffeau A., Gonzalez A., Grothues D., Guerreiro P., Hegemann J.H., Hewitt N., Hilger F., Hollenberg C.P., Horaitis O., Indge K.J., Jacquier A., James C.M., Jauniaux J.-C., Jimenez A., Keuchel H., Kirchrath L., Kleine K., Koetter P., Legrain P., Liebl S., Louis E.J., Maia e Silva A., Marck C., Monnier A.-L., Moestl D., Mueller S., Obermaier B., Oliver S.G., Pallier C., Pascolo S., Pfeiffer F., Philippsen P., Planta R.J., Pohl F.M., Pohl T.M., Poehlmann R., Portetelle D., Purnelle B., Puzos V., Ramezani Rad M., Rasmussen S.W., Remacha M.A., Revuelta J.L., Richard G.-F., Rieger M., Rodrigues-Pousada C., Rose M., Rupp T., Santos M.A., Schwager C., Sensen C., Skala J., Soares H., Sor F., Stegemann J., Tettelin H., Thierry A., Tzermia M., Urrestarazu L.A., van Dyck L., van Vliet-Reedijk J.C., Valens M., Vandenbol M., Vilela C., Vissers S., von Wettstein D., Voss H., Wiemann S., Xu G., Zimmermann J., Haasemann M., Becker I., Mewes H.-W. Nature 369:371-378(1994) [PubMed: 8196765] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[3]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[4]	"Sequencing and analysis of a 20.5 kb DNA segment located on the left arm of yeast chromosome XI." Vandenbol M., Bolle P.-A., Dion C., Portetelle D., Hilger F. Yeast 10:S25-S33(1994) [PubMed: 8091858] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-306. Strain: ATCC 204508 / S288c.
[5]	"Control of amino acid permease sorting in the late secretory pathway of Saccharomyces cerevisiae by SEC13, LST4, LST7 and LST8." Roberg K.J., Bickel S., Rowley N., Kaiser C.A. Genetics 147:1569-1584(1997) [PubMed: 9409822] [Abstract] Cited for: FUNCTION.
[6]	"Components of a ubiquitin ligase complex specify polyubiquitination and intracellular trafficking of the general amino acid permease." Helliwell S.B., Losko S., Kaiser C.A. J. Cell Biol. 153:649-662(2001) [PubMed: 11352928] [Abstract] Cited for: FUNCTION.
[7]	"Amino acids regulate retrieval of the yeast general amino acid permease from the vacuolar targeting pathway." Rubio-Texeira M., Kaiser C.A. Mol. Biol. Cell 17:3031-3050(2006) [PubMed: 16641373] [Abstract] Cited for: FUNCTION.
[8]	"Proteome-wide identification of in vivo targets of DNA damage checkpoint kinases." Smolka M.B., Albuquerque C.P., Chen S.H., Zhou H. Proc. Natl. Acad. Sci. U.S.A. 104:10364-10369(2007) [PubMed: 17563356] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-418, MASS SPECTROMETRY.
[9]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed: 18407956] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-547, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X74151 Genomic DNA. Translation: CAA52262.1. Z26878 Genomic DNA. Translation: CAA81509.1. Z28176 Genomic DNA. Translation: CAA82018.1. BK006944 Genomic DNA. Translation: DAA08990.1.
PIR	S34695.
RefSeq	NP_012745.1. NM_001179742.1.
3D structure databases
ProteinModelPortal	P34239.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-2882N.
IntAct	P34239. 2 interactions.
MINT	MINT-681267.
STRING	P34239.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YKL176C; YKL176C; YKL176C.
GeneID	853678.
KEGG	sce:YKL176C.
NMPDR	fig|4932.3.peg.3723.
Organism-specific databases
CYGD	YKL176c.
SGD	S000001659. LST4.
Phylogenomic databases
eggNOG	fuNOG08171.
HOGENOM	HBG202403.
OMA	GSYGSWF.
OrthoDB	EOG4HMNK7.
Gene expression databases
ArrayExpress	P34239.
Genevestigator	P34239.
GermOnline	YKL176C. Saccharomyces cerevisiae.
Family and domain databases
ProtoNet	Search...
Other
NextBio	974634.

Entry information

Entry name

LST4_YEAST

Accession

Primary (citable) accession number: P34239
Secondary accession number(s): D6VX24

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1994
Last sequence update:	February 1, 1994
Last modified:	December 14, 2011
This is version 73 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program