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Protein

Transcriptional regulatory protein ASH1

Gene

ASH1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Component of the RPD3C(L) histone deacetylase complex (HDAC). Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. ASH1 is necessary to repress HO in daughter cells to block mating-type switching through its binding to HO promoter 5'-YTGAT-3' sites. Also involved in pseudohyphal growth.4 Publications

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri499 – 526GATA-type; atypicalPROSITE-ProRule annotationAdd BLAST28

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Chromatin regulator, Repressor

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding, Metal-binding, Zinc

Enzyme and pathway databases

BioCyciYEAST:G3O-31948-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Transcriptional regulatory protein ASH1
Alternative name(s):
Daughter cells HO repressor protein
Gene namesi
Name:ASH1
Ordered Locus Names:YKL185W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XI

Organism-specific databases

EuPathDBiFungiDB:YKL185W.
SGDiS000001668. ASH1.

Subcellular locationi

GO - Cellular componenti

  • cellular bud Source: SGD
  • nucleus Source: SGD
  • Rpd3L complex Source: SGD
  • transcription factor complex Source: GO_Central
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000834921 – 588Transcriptional regulatory protein ASH1Add BLAST588

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei56PhosphoserineCombined sources1
Modified residuei465PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP34233.
PRIDEiP34233.

PTM databases

iPTMnetiP34233.

Interactioni

Subunit structurei

Component of the RPD3C(L) complex composed of at least ASH1, CTI6, DEP1, PHO23, RPD3, RXT2, RXT3, SAP30, SDS3, SIN3, UME1 and UME6.1 Publication

GO - Molecular functioni

Protein-protein interaction databases

BioGridi33937. 75 interactors.
DIPiDIP-8053N.
IntActiP34233. 9 interactors.
MINTiMINT-2783083.

Structurei

3D structure databases

ProteinModelPortaliP34233.
SMRiP34233.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Contains 1 GATA-type zinc finger.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri499 – 526GATA-type; atypicalPROSITE-ProRule annotationAdd BLAST28

Keywords - Domaini

Zinc-finger

Phylogenomic databases

HOGENOMiHOG000034095.
InParanoidiP34233.
KOiK18658.
OMAiGYRCLFC.
OrthoDBiEOG092C4M94.

Family and domain databases

Gene3Di3.30.50.10. 1 hit.
InterProiIPR000679. Znf_GATA.
IPR013088. Znf_NHR/GATA.
[Graphical view]
PfamiPF00320. GATA. 1 hit.
[Graphical view]
SMARTiSM00401. ZnF_GATA. 1 hit.
[Graphical view]
PROSITEiPS00344. GATA_ZN_FINGER_1. 1 hit.
PS50114. GATA_ZN_FINGER_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P34233-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSSLYIKTPL HALSAGPDSH ANSSYYDNLL LPSFSNLSSN ISRNNITTDN
60 70 80 90 100
NINSASPRKY SFHSLNVSPI LSPISLANEI LGKKSNTAPA SPHHMDYNPI
110 120 130 140 150
SSLTPGNSPE FNKASLSQIS FTNPLNYGSG LGFSSNSQPR LPLLDRLSSV
160 170 180 190 200
SLSKRPERPQ QSLPSLRHLQ LLPSPLLQEN AARFPDTSKR TSNWKTDLTH
210 220 230 240 250
WCKDTNYQDY VKIREEVAHF KPLSIPNLTN NQNNDSFNYG KELESTRSSK
260 270 280 290 300
FHSPSKESFD RTKLIPSILE AKDQFKDLSN NAWSITPPVT PPMSPPTNRT
310 320 330 340 350
MERTTLRGVE ASFFEGKSSN NDSIFNPIIS EKLVQEVKHQ RQLRGNSFPM
360 370 380 390 400
PNASHKKTNS FKALQIKKLL ANRDILSNNS KSNVRKPSKN KISKQASNVF
410 420 430 440 450
GNTARQLVMK LDNASYSSVS ASSSPSPSTP TKSGKMRSRS SSPVRPKAYT
460 470 480 490 500
PSPRSPNYHR FALDSPPQSP RRSSNSSITK KGSRRSSGSS PTRHTTRVCV
510 520 530 540 550
SCHSSDSPCW RPSWSPRKQD QLCNSCGLRY KKTHTRCLND LCRKIPTKGE
560 570 580
INIMKSNGID KEFVPERNCE IEGYRCLFCN YITETVEN
Length:588
Mass (Da):65,685
Last modified:February 1, 1994 - v1
Checksum:i7DFFD1B9392DAC08
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X74151 Genomic DNA. Translation: CAA52253.1.
Z28185 Genomic DNA. Translation: CAA82028.1.
M88605 Genomic DNA. Translation: AAA34830.1.
BK006944 Genomic DNA. Translation: DAA08981.1.
PIRiS34685.
RefSeqiNP_012736.1. NM_001179751.1.

Genome annotation databases

EnsemblFungiiYKL185W; YKL185W; YKL185W.
GeneIDi853650.
KEGGisce:YKL185W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X74151 Genomic DNA. Translation: CAA52253.1.
Z28185 Genomic DNA. Translation: CAA82028.1.
M88605 Genomic DNA. Translation: AAA34830.1.
BK006944 Genomic DNA. Translation: DAA08981.1.
PIRiS34685.
RefSeqiNP_012736.1. NM_001179751.1.

3D structure databases

ProteinModelPortaliP34233.
SMRiP34233.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi33937. 75 interactors.
DIPiDIP-8053N.
IntActiP34233. 9 interactors.
MINTiMINT-2783083.

PTM databases

iPTMnetiP34233.

Proteomic databases

MaxQBiP34233.
PRIDEiP34233.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYKL185W; YKL185W; YKL185W.
GeneIDi853650.
KEGGisce:YKL185W.

Organism-specific databases

EuPathDBiFungiDB:YKL185W.
SGDiS000001668. ASH1.

Phylogenomic databases

HOGENOMiHOG000034095.
InParanoidiP34233.
KOiK18658.
OMAiGYRCLFC.
OrthoDBiEOG092C4M94.

Enzyme and pathway databases

BioCyciYEAST:G3O-31948-MONOMER.

Miscellaneous databases

PROiP34233.

Family and domain databases

Gene3Di3.30.50.10. 1 hit.
InterProiIPR000679. Znf_GATA.
IPR013088. Znf_NHR/GATA.
[Graphical view]
PfamiPF00320. GATA. 1 hit.
[Graphical view]
SMARTiSM00401. ZnF_GATA. 1 hit.
[Graphical view]
PROSITEiPS00344. GATA_ZN_FINGER_1. 1 hit.
PS50114. GATA_ZN_FINGER_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiASH1_YEAST
AccessioniPrimary (citable) accession number: P34233
Secondary accession number(s): D6VX15
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: February 1, 1994
Last modified: November 2, 2016
This is version 141 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

The ASH1 mRNA is transported to the daughter cell before cytokinesis where translation produces the protein to block mating-type switching. The ASH1 mRNA 3'-UTR and the mRNA localization machinery that are essential to restrict accumulation to the bud.
Present with 1800 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XI
    Yeast (Saccharomyces cerevisiae) chromosome XI: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.