SubmitCancel

Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

P34233

- ASH1_YEAST

UniProt

P34233 - ASH1_YEAST

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein

Transcriptional regulatory protein ASH1

Gene
ASH1, YKL185W
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Component of the RPD3C(L) histone deacetylase complex (HDAC). Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. ASH1 is necessary to repress HO in daughter cells to block mating-type switching through its binding to HO promoter 5'-YTGAT-3' sites. Also involved in pseudohyphal growth.4 Publications

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri499 – 52628GATA-type; atypicalAdd
BLAST

GO - Molecular functioni

  1. core promoter proximal region sequence-specific DNA binding Source: SGD
  2. RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in negative regulation of transcription Source: SGD
  3. zinc ion binding Source: InterPro

GO - Biological processi

  1. chromatin modification Source: UniProtKB-KW
  2. negative regulation of G1/S transition of mitotic cell cycle by negative regulation of transcription from RNA polymerase II promoter Source: SGD
  3. negative regulation of mating type switching by negative regulation of transcription from RNA polymerase II promoter Source: SGD
  4. positive regulation of pseudohyphal growth by positive regulation of transcription from RNA polymerase II promoter Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Chromatin regulator, Repressor

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding, Metal-binding, Zinc

Enzyme and pathway databases

BioCyciYEAST:G3O-31948-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Transcriptional regulatory protein ASH1
Alternative name(s):
Daughter cells HO repressor protein
Gene namesi
Name:ASH1
Ordered Locus Names:YKL185W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome XI

Organism-specific databases

CYGDiYKL185w.
SGDiS000001668. ASH1.

Subcellular locationi

Nucleus
Note: Preferentially accumulates in daughter cell nuclei at the end of anaphase.5 Publications

GO - Cellular componenti

  1. cellular bud Source: SGD
  2. nucleus Source: SGD
  3. Rpd3L complex Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 588588Transcriptional regulatory protein ASH1PRO_0000083492Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei56 – 561Phosphoserine1 Publication
Modified residuei465 – 4651Phosphoserine2 Publications

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP34233.
PaxDbiP34233.

Expressioni

Gene expression databases

GenevestigatoriP34233.

Interactioni

Subunit structurei

Component of the RPD3C(L) complex composed of at least ASH1, CTI6, DEP1, PHO23, RPD3, RXT2, RXT3, SAP30, SDS3, SIN3, UME1 and UME6.1 Publication

Protein-protein interaction databases

BioGridi33937. 76 interactions.
DIPiDIP-8053N.
IntActiP34233. 9 interactions.
MINTiMINT-2783083.
STRINGi4932.YKL185W.

Structurei

3D structure databases

ProteinModelPortaliP34233.
SMRiP34233. Positions 498-535.

Family & Domainsi

Sequence similaritiesi

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri499 – 52628GATA-type; atypicalAdd
BLAST

Keywords - Domaini

Zinc-finger

Phylogenomic databases

eggNOGiNOG44003.
HOGENOMiHOG000034095.
OMAiPERNCEI.
OrthoDBiEOG7M3J86.

Family and domain databases

Gene3Di3.30.50.10. 1 hit.
InterProiIPR000679. Znf_GATA.
IPR013088. Znf_NHR/GATA.
[Graphical view]
PfamiPF00320. GATA. 1 hit.
[Graphical view]
SMARTiSM00401. ZnF_GATA. 1 hit.
[Graphical view]
PROSITEiPS00344. GATA_ZN_FINGER_1. 1 hit.
PS50114. GATA_ZN_FINGER_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P34233-1 [UniParc]FASTAAdd to Basket

« Hide

MSSLYIKTPL HALSAGPDSH ANSSYYDNLL LPSFSNLSSN ISRNNITTDN    50
NINSASPRKY SFHSLNVSPI LSPISLANEI LGKKSNTAPA SPHHMDYNPI 100
SSLTPGNSPE FNKASLSQIS FTNPLNYGSG LGFSSNSQPR LPLLDRLSSV 150
SLSKRPERPQ QSLPSLRHLQ LLPSPLLQEN AARFPDTSKR TSNWKTDLTH 200
WCKDTNYQDY VKIREEVAHF KPLSIPNLTN NQNNDSFNYG KELESTRSSK 250
FHSPSKESFD RTKLIPSILE AKDQFKDLSN NAWSITPPVT PPMSPPTNRT 300
MERTTLRGVE ASFFEGKSSN NDSIFNPIIS EKLVQEVKHQ RQLRGNSFPM 350
PNASHKKTNS FKALQIKKLL ANRDILSNNS KSNVRKPSKN KISKQASNVF 400
GNTARQLVMK LDNASYSSVS ASSSPSPSTP TKSGKMRSRS SSPVRPKAYT 450
PSPRSPNYHR FALDSPPQSP RRSSNSSITK KGSRRSSGSS PTRHTTRVCV 500
SCHSSDSPCW RPSWSPRKQD QLCNSCGLRY KKTHTRCLND LCRKIPTKGE 550
INIMKSNGID KEFVPERNCE IEGYRCLFCN YITETVEN 588
Length:588
Mass (Da):65,685
Last modified:February 1, 1994 - v1
Checksum:i7DFFD1B9392DAC08
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X74151 Genomic DNA. Translation: CAA52253.1.
Z28185 Genomic DNA. Translation: CAA82028.1.
M88605 Genomic DNA. Translation: AAA34830.1.
BK006944 Genomic DNA. Translation: DAA08981.1.
PIRiS34685.
RefSeqiNP_012736.1. NM_001179751.1.

Genome annotation databases

EnsemblFungiiYKL185W; YKL185W; YKL185W.
GeneIDi853650.
KEGGisce:YKL185W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X74151 Genomic DNA. Translation: CAA52253.1 .
Z28185 Genomic DNA. Translation: CAA82028.1 .
M88605 Genomic DNA. Translation: AAA34830.1 .
BK006944 Genomic DNA. Translation: DAA08981.1 .
PIRi S34685.
RefSeqi NP_012736.1. NM_001179751.1.

3D structure databases

ProteinModelPortali P34233.
SMRi P34233. Positions 498-535.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 33937. 76 interactions.
DIPi DIP-8053N.
IntActi P34233. 9 interactions.
MINTi MINT-2783083.
STRINGi 4932.YKL185W.

Proteomic databases

MaxQBi P34233.
PaxDbi P34233.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YKL185W ; YKL185W ; YKL185W .
GeneIDi 853650.
KEGGi sce:YKL185W.

Organism-specific databases

CYGDi YKL185w.
SGDi S000001668. ASH1.

Phylogenomic databases

eggNOGi NOG44003.
HOGENOMi HOG000034095.
OMAi PERNCEI.
OrthoDBi EOG7M3J86.

Enzyme and pathway databases

BioCyci YEAST:G3O-31948-MONOMER.

Miscellaneous databases

NextBioi 974560.

Gene expression databases

Genevestigatori P34233.

Family and domain databases

Gene3Di 3.30.50.10. 1 hit.
InterProi IPR000679. Znf_GATA.
IPR013088. Znf_NHR/GATA.
[Graphical view ]
Pfami PF00320. GATA. 1 hit.
[Graphical view ]
SMARTi SM00401. ZnF_GATA. 1 hit.
[Graphical view ]
PROSITEi PS00344. GATA_ZN_FINGER_1. 1 hit.
PS50114. GATA_ZN_FINGER_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Sequencing and analysis of 51.6 kilobases on the left arm of chromosome XI from Saccharomyces cerevisiae reveals 23 open reading frames including the FAS1 gene."
    Wiemann S., Voss H., Schwager C., Rupp T., Stegemann J., Zimmermann J., Grothues D., Sensen C., Erfle H., Hewitt N., Banrevi A., Ansorge W.
    Yeast 9:1343-1348(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "Complete DNA sequence of yeast chromosome XI."
    Dujon B., Alexandraki D., Andre B., Ansorge W., Baladron V., Ballesta J.P.G., Banrevi A., Bolle P.-A., Bolotin-Fukuhara M., Bossier P., Bou G., Boyer J., Buitrago M.J., Cheret G., Colleaux L., Daignan-Fornier B., del Rey F., Dion C.
    , Domdey H., Duesterhoeft A., Duesterhus S., Entian K.-D., Erfle H., Esteban P.F., Feldmann H., Fernandes L., Fobo G.M., Fritz C., Fukuhara H., Gabel C., Gaillon L., Garcia-Cantalejo J.M., Garcia-Ramirez J.J., Gent M.E., Ghazvini M., Goffeau A., Gonzalez A., Grothues D., Guerreiro P., Hegemann J.H., Hewitt N., Hilger F., Hollenberg C.P., Horaitis O., Indge K.J., Jacquier A., James C.M., Jauniaux J.-C., Jimenez A., Keuchel H., Kirchrath L., Kleine K., Koetter P., Legrain P., Liebl S., Louis E.J., Maia e Silva A., Marck C., Monnier A.-L., Moestl D., Mueller S., Obermaier B., Oliver S.G., Pallier C., Pascolo S., Pfeiffer F., Philippsen P., Planta R.J., Pohl F.M., Pohl T.M., Poehlmann R., Portetelle D., Purnelle B., Puzos V., Ramezani Rad M., Rasmussen S.W., Remacha M.A., Revuelta J.L., Richard G.-F., Rieger M., Rodrigues-Pousada C., Rose M., Rupp T., Santos M.A., Schwager C., Sensen C., Skala J., Soares H., Sor F., Stegemann J., Tettelin H., Thierry A., Tzermia M., Urrestarazu L.A., van Dyck L., van Vliet-Reedijk J.C., Valens M., Vandenbol M., Vilela C., Vissers S., von Wettstein D., Voss H., Wiemann S., Xu G., Zimmermann J., Haasemann M., Becker I., Mewes H.-W.
    Nature 369:371-378(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  4. Cusick M.E.
    Submitted (JUN-1992) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 110-563.
  5. "Asymmetric accumulation of Ash1p in postanaphase nuclei depends on a myosin and restricts yeast mating-type switching to mother cells."
    Bobola N., Jansen R.-P., Shin T.H., Nasmyth K.
    Cell 84:699-709(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION.
  6. "Identification of asymmetrically localized determinant, Ash1p, required for lineage-specific transcription of the yeast HO gene."
    Sil A., Herskowitz I.
    Cell 84:711-722(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION.
  7. "Actin-dependent localization of an RNA encoding a cell-fate determinant in yeast."
    Takizawa P.A., Sil A., Swedlow J.R., Herskowitz I., Vale R.D.
    Nature 389:90-93(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: TRANSPORT AND ASYMMETRIC LOCATION OF MRNA.
  8. "Mating type switching in yeast controlled by asymmetric localization of ASH1 mRNA."
    Long R.M., Singer R.H., Meng X., Gonzalez I., Nasmyth K., Jansen R.-P.
    Science 277:383-387(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: TRANSPORT AND ASYMMETRIC LOCATION OF MRNA.
  9. Cited for: SUBCELLULAR LOCATION, MRNA TRANSPORT.
  10. "Ash1, a daughter cell-specific protein, is required for pseudohyphal growth of Saccharomyces cerevisiae."
    Chandarlapaty S., Errede B.
    Mol. Cell. Biol. 18:2884-2891(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION.
  11. "Ash1p is a site-specific DNA-binding protein that actively represses transcription."
    Maxon M.E., Herskowitz I.
    Proc. Natl. Acad. Sci. U.S.A. 98:1495-1500(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, DOMAIN, DNA-BINDING.
  12. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
  13. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  14. "Stable incorporation of sequence specific repressors Ash1 and Ume6 into the Rpd3L complex."
    Carrozza M.J., Florens L., Swanson S.K., Shia W.-J., Anderson S., Yates J., Washburn M.P., Workman J.L.
    Biochim. Biophys. Acta 1731:77-87(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION IN THE RPD3C(L) COMPLEX, IDENTIFICATION BY MASS SPECTROMETRY.
  15. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
    Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
    Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-465, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  16. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
    Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
    Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-56 AND SER-465, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiASH1_YEAST
AccessioniPrimary (citable) accession number: P34233
Secondary accession number(s): D6VX15
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: February 1, 1994
Last modified: June 11, 2014
This is version 122 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

The ASH1 mRNA is transported to the daughter cell before cytokinesis where translation produces the protein to block mating-type switching. The ASH1 mRNA 3'-UTR and the mRNA localization machinery that are essential to restrict accumulation to the bud.
Present with 1800 molecules/cell in log phase SD medium.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XI
    Yeast (Saccharomyces cerevisiae) chromosome XI: entries and gene names

External Data

Dasty 3

Similar proteinsi