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Protein

Transcriptional regulatory protein ASH1

Gene

ASH1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Component of the RPD3C(L) histone deacetylase complex (HDAC). Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. ASH1 is necessary to repress HO in daughter cells to block mating-type switching through its binding to HO promoter 5'-YTGAT-3' sites. Also involved in pseudohyphal growth.4 Publications

Miscellaneous

The ASH1 mRNA is transported to the daughter cell before cytokinesis where translation produces the protein to block mating-type switching. The ASH1 mRNA 3'-UTR and the mRNA localization machinery that are essential to restrict accumulation to the bud.
Present with 1800 molecules/cell in log phase SD medium.1 Publication

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri499 – 526GATA-type; atypicalPROSITE-ProRule annotationAdd BLAST28

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionChromatin regulator, DNA-binding, Repressor
Biological processTranscription, Transcription regulation
LigandMetal-binding, Zinc

Enzyme and pathway databases

BioCyciYEAST:G3O-31948-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Transcriptional regulatory protein ASH1
Alternative name(s):
Daughter cells HO repressor protein
Gene namesi
Name:ASH1
Ordered Locus Names:YKL185W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XI

Organism-specific databases

EuPathDBiFungiDB:YKL185W
SGDiS000001668 ASH1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000834921 – 588Transcriptional regulatory protein ASH1Add BLAST588

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei56PhosphoserineCombined sources1
Modified residuei465PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP34233
PaxDbiP34233
PRIDEiP34233

PTM databases

iPTMnetiP34233

Interactioni

Subunit structurei

Component of the RPD3C(L) complex composed of at least ASH1, CTI6, DEP1, PHO23, RPD3, RXT2, RXT3, SAP30, SDS3, SIN3, UME1 and UME6.1 Publication

GO - Molecular functioni

Protein-protein interaction databases

BioGridi33937, 150 interactors
DIPiDIP-8053N
IntActiP34233, 9 interactors
MINTiP34233
STRINGi4932.YKL185W

Structurei

3D structure databases

ProteinModelPortaliP34233
SMRiP34233
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri499 – 526GATA-type; atypicalPROSITE-ProRule annotationAdd BLAST28

Keywords - Domaini

Zinc-finger

Phylogenomic databases

HOGENOMiHOG000034095
InParanoidiP34233
KOiK18658
OMAiKKTHTRC
OrthoDBiEOG092C4M94

Family and domain databases

Gene3Di3.30.50.10, 1 hit
InterProiView protein in InterPro
IPR000679 Znf_GATA
IPR013088 Znf_NHR/GATA
PfamiView protein in Pfam
PF00320 GATA, 1 hit
SMARTiView protein in SMART
SM00401 ZnF_GATA, 1 hit
PROSITEiView protein in PROSITE
PS00344 GATA_ZN_FINGER_1, 1 hit
PS50114 GATA_ZN_FINGER_2, 1 hit

Sequencei

Sequence statusi: Complete.

P34233-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSSLYIKTPL HALSAGPDSH ANSSYYDNLL LPSFSNLSSN ISRNNITTDN
60 70 80 90 100
NINSASPRKY SFHSLNVSPI LSPISLANEI LGKKSNTAPA SPHHMDYNPI
110 120 130 140 150
SSLTPGNSPE FNKASLSQIS FTNPLNYGSG LGFSSNSQPR LPLLDRLSSV
160 170 180 190 200
SLSKRPERPQ QSLPSLRHLQ LLPSPLLQEN AARFPDTSKR TSNWKTDLTH
210 220 230 240 250
WCKDTNYQDY VKIREEVAHF KPLSIPNLTN NQNNDSFNYG KELESTRSSK
260 270 280 290 300
FHSPSKESFD RTKLIPSILE AKDQFKDLSN NAWSITPPVT PPMSPPTNRT
310 320 330 340 350
MERTTLRGVE ASFFEGKSSN NDSIFNPIIS EKLVQEVKHQ RQLRGNSFPM
360 370 380 390 400
PNASHKKTNS FKALQIKKLL ANRDILSNNS KSNVRKPSKN KISKQASNVF
410 420 430 440 450
GNTARQLVMK LDNASYSSVS ASSSPSPSTP TKSGKMRSRS SSPVRPKAYT
460 470 480 490 500
PSPRSPNYHR FALDSPPQSP RRSSNSSITK KGSRRSSGSS PTRHTTRVCV
510 520 530 540 550
SCHSSDSPCW RPSWSPRKQD QLCNSCGLRY KKTHTRCLND LCRKIPTKGE
560 570 580
INIMKSNGID KEFVPERNCE IEGYRCLFCN YITETVEN
Length:588
Mass (Da):65,685
Last modified:February 1, 1994 - v1
Checksum:i7DFFD1B9392DAC08
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X74151 Genomic DNA Translation: CAA52253.1
Z28185 Genomic DNA Translation: CAA82028.1
M88605 Genomic DNA Translation: AAA34830.1
BK006944 Genomic DNA Translation: DAA08981.1
PIRiS34685
RefSeqiNP_012736.1, NM_001179751.1

Genome annotation databases

EnsemblFungiiYKL185W; YKL185W; YKL185W
GeneIDi853650
KEGGisce:YKL185W

Similar proteinsi

Entry informationi

Entry nameiASH1_YEAST
AccessioniPrimary (citable) accession number: P34233
Secondary accession number(s): D6VX15
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: February 1, 1994
Last modified: May 23, 2018
This is version 154 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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