P34232 (MTR2_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
mRNA transport regulator MTR2

Gene names

Name:	MTR2
Ordered Locus Names:	YKL186C

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	184 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Affects mRNA transport from the nucleus to the cytoplasm.
Subunit structure	Interacts with MEX67.
Subcellular location	Nucleus.

Ontologies

Keywords
Biological process	Transport
Cellular component	Nucleus
PTM	Phosphoprotein
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	poly(A)+ mRNA export from nucleus Inferred from mutant phenotype Ref.1. Source: SGD ribosomal large subunit export from nucleus Inferred from mutant phenotype. Source: SGD
Cellular component	nuclear RNA export factor complex Inferred from physical interaction. Source: SGD nuclear pore Inferred from direct assay. Source: SGD
Molecular function	protein binding Inferred from physical interaction. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 184

184

mRNA transport regulator MTR2

PRO_0000096634

Amino acid modifications

Modified residue

125

1

Phosphothreonine Ref.6

Secondary structure

1

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184

Helix Strand Turn

Details...

Helix

15 – 31

17

Helix

39 – 42

4

Turn

43 – 44

2

Beta strand

52 – 54

3

Turn

55 – 56

2

Beta strand

57 – 60

4

Helix

62 – 72

11

Beta strand

77 – 88

12

Turn

89 – 92

4

Beta strand

93 – 103

11

Beta strand

145 – 154

10

Helix

155 – 159

5

Turn

160

1

Turn

163 – 164

2

Beta strand

166 – 175

10

Sequences

	Sequence		Length	Mass (Da)
	P34232 [UniParc]. Last modified February 1, 1994. Version 1. Checksum: D3AE487A11FA2750	FASTA	184	20,784
	10 20 30 40 50 60 MNTNSNTMVM NDANQAQITA TFTKKILAHL DDPDSNKLAQ FVQLFNPNNC RIIFNATPFA 70 80 90 100 110 120 QATVFLQMWQ NQVVQTQHAL TGVDYHAIPG SGTLICNVNC KVRFDESGRD KMGQDATVPI 130 140 150 160 170 180 QPNNTGNRNR PNDMNKPRPL WGPYFGISLQ LIIDDRIFRN DFNGVISGFN YNMVYKPEDS LLKI

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Nuclear mRNA accumulation causes nucleolar fragmentation in yeast mtr2 mutant." Kadowaki T., Hitomi M., Chen S., Tartakoff A.M. Mol. Biol. Cell 5:1253-1263(1994) [PubMed: 7865887] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Sequencing and analysis of 51.6 kilobases on the left arm of chromosome XI from Saccharomyces cerevisiae reveals 23 open reading frames including the FAS1 gene." Wiemann S., Voss H., Schwager C., Rupp T., Stegemann J., Zimmermann J., Grothues D., Sensen C., Erfle H., Hewitt N., Banrevi A., Ansorge W. Yeast 9:1343-1348(1993) [PubMed: 8154185] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"Complete DNA sequence of yeast chromosome XI." Dujon B., Alexandraki D., Andre B., Ansorge W., Baladron V., Ballesta J.P.G., Banrevi A., Bolle P.-A., Bolotin-Fukuhara M., Bossier P., Bou G., Boyer J., Buitrago M.J., Cheret G., Colleaux L., Daignan-Fornier B., del Rey F., Dion C. , Domdey H., Duesterhoeft A., Duesterhus S., Entian K.-D., Erfle H., Esteban P.F., Feldmann H., Fernandes L., Fobo G.M., Fritz C., Fukuhara H., Gabel C., Gaillon L., Garcia-Cantalejo J.M., Garcia-Ramirez J.J., Gent M.E., Ghazvini M., Goffeau A., Gonzalez A., Grothues D., Guerreiro P., Hegemann J.H., Hewitt N., Hilger F., Hollenberg C.P., Horaitis O., Indge K.J., Jacquier A., James C.M., Jauniaux J.-C., Jimenez A., Keuchel H., Kirchrath L., Kleine K., Koetter P., Legrain P., Liebl S., Louis E.J., Maia e Silva A., Marck C., Monnier A.-L., Moestl D., Mueller S., Obermaier B., Oliver S.G., Pallier C., Pascolo S., Pfeiffer F., Philippsen P., Planta R.J., Pohl F.M., Pohl T.M., Poehlmann R., Portetelle D., Purnelle B., Puzos V., Ramezani Rad M., Rasmussen S.W., Remacha M.A., Revuelta J.L., Richard G.-F., Rieger M., Rodrigues-Pousada C., Rose M., Rupp T., Santos M.A., Schwager C., Sensen C., Skala J., Soares H., Sor F., Stegemann J., Tettelin H., Thierry A., Tzermia M., Urrestarazu L.A., van Dyck L., van Vliet-Reedijk J.C., Valens M., Vandenbol M., Vilela C., Vissers S., von Wettstein D., Voss H., Wiemann S., Xu G., Zimmermann J., Haasemann M., Becker I., Mewes H.-W. Nature 369:371-378(1994) [PubMed: 8196765] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[4]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[5]	"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae." Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J. Genome Res. 17:536-543(2007) [PubMed: 17322287] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[6]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed: 18407956] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-125, MASS SPECTROMETRY.
[7]	"Structural similarity in the absence of sequence homology of the messenger RNA export factors Mtr2 and p15." Fribourg S., Conti E. EMBO Rep. 4:699-703(2003) [PubMed: 12835756] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.8 ANGSTROMS) OF 14-178 IN COMPLEX WITH MEX67.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

S77467 Genomic DNA. Translation: AAB33484.1.
X74151 Genomic DNA. Translation: CAA52252.1.
Z28186 Genomic DNA. Translation: CAA82029.1.
AY558555 Genomic DNA. Translation: AAS56881.1.
BK006944 Genomic DNA. Translation: DAA08980.1.

PIR

S34684.

RefSeq

NP_012735.1. NM_001179752.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1OF5	X-ray	2.80	B	1-184	[»]

ProteinModelPortal

P34232.

SMR

P34232. Positions 14-178.

ModBase

Search...

Protein-protein interaction databases

DIP

DIP-4856N.

IntAct

P34232. 6 interactions.

MINT

MINT-496382.

STRING

P34232.

Protein family/group databases

TCDB

9.A.14.1.1. nuclear pore complex (NPC) family.

Proteomic databases

PeptideAtlas

P34232.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblFungi

YKL186C; YKL186C; YKL186C.

GeneID

853649.

KEGG

sce:YKL186C.

NMPDR

fig|4932.3.peg.3712.

Organism-specific databases

CYGD

YKL186c1.

SGD

S000001669. MTR2.

Phylogenomic databases

eggNOG

fuNOG10737.

HOGENOM

HBG398436.

OMA

VNCKVRF.

OrthoDB

EOG4C2MM3.

Gene expression databases

ArrayExpress

P34232.

Genevestigator

P34232.

GermOnline

YKL186C. Saccharomyces cerevisiae.

Family and domain databases

InterPro

IPR019488. Nucl_pore_RNA_shuttling_Mtr2.
[Graphical view]

KO

K14543.

Pfam

PF10429. Mtr2. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

NextBio

974557.

Entry information

Entry name

MTR2_YEAST

Accession

Primary (citable) accession number: P34232
Secondary accession number(s): D6VX14

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1994
Last sequence update:	February 1, 1994
Last modified:	December 14, 2011
This is version 87 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program