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Protein

Peroxisomal long-chain fatty acid import protein 1

Gene

PXA2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Involved in the import of activated long-chain fatty acids from the cytosol to the peroxisomal matrix.1 Publication

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi505 – 5128ATPPROSITE-ProRule annotation

GO - Molecular functioni

  • ATPase activity, coupled to transmembrane movement of substances Source: SGD
  • ATP binding Source: UniProtKB-KW
  • peroxisomal fatty-acyl-CoA transporter activity Source: UniProtKB

GO - Biological processi

  • fatty acid beta-oxidation Source: UniProtKB
  • fatty acid transmembrane transport Source: SGD
  • fatty-acyl-CoA transport Source: UniProtKB
  • long-chain fatty acid catabolic process Source: UniProtKB
  • peroxisomal long-chain fatty acid import Source: UniProtKB
  • very long-chain fatty acid catabolic process Source: UniProtKB
Complete GO annotation...

Keywords - Biological processi

Transport

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-31951-MONOMER.
BRENDAi3.6.3.47. 984.

Protein family/group databases

TCDBi3.A.1.203.2. the atp-binding cassette (abc) superfamily.
3.A.1.203.6. the atp-binding cassette (abc) superfamily.

Names & Taxonomyi

Protein namesi
Recommended name:
Peroxisomal long-chain fatty acid import protein 1
Alternative name(s):
Peroxisomal ABC transporter 2
Gene namesi
Name:PXA2
Synonyms:PAT1
Ordered Locus Names:YKL188C
ORF Names:YKL741
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XI

Organism-specific databases

EuPathDBiFungiDB:YKL188C.
SGDiS000001671. PXA2.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei122 – 14221HelicalPROSITE-ProRule annotationAdd
BLAST
Transmembranei166 – 18621HelicalPROSITE-ProRule annotationAdd
BLAST
Transmembranei269 – 28921HelicalPROSITE-ProRule annotationAdd
BLAST
Transmembranei364 – 38421HelicalPROSITE-ProRule annotationAdd
BLAST

GO - Cellular componenti

  • integral component of peroxisomal membrane Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Membrane, Peroxisome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 853853Peroxisomal long-chain fatty acid import protein 1PRO_0000093315Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi33 – 331N-linked (GlcNAc...)Sequence analysis
Glycosylationi39 – 391N-linked (GlcNAc...)Sequence analysis
Glycosylationi162 – 1621N-linked (GlcNAc...)Sequence analysis
Glycosylationi230 – 2301N-linked (GlcNAc...)Sequence analysis
Glycosylationi241 – 2411N-linked (GlcNAc...)Sequence analysis
Glycosylationi267 – 2671N-linked (GlcNAc...)Sequence analysis
Glycosylationi295 – 2951N-linked (GlcNAc...)Sequence analysis
Glycosylationi560 – 5601N-linked (GlcNAc...)Sequence analysis
Glycosylationi608 – 6081N-linked (GlcNAc...)Sequence analysis
Glycosylationi620 – 6201N-linked (GlcNAc...)Sequence analysis
Glycosylationi647 – 6471N-linked (GlcNAc...)Sequence analysis
Glycosylationi836 – 8361N-linked (GlcNAc...)Sequence analysis

Keywords - PTMi

Glycoprotein

Proteomic databases

PeptideAtlasiP34230.
TopDownProteomicsiP34230.

Interactioni

Subunit structurei

Forms a heterodimer with PXA1.

Binary interactionsi

WithEntry#Exp.IntActNotes
PXA1P419092EBI-2464632,EBI-14344

Protein-protein interaction databases

BioGridi33934. 76 interactions.
DIPiDIP-741N.
IntActiP34230. 1 interaction.
MINTiMINT-1164061.

Structurei

3D structure databases

ProteinModelPortaliP34230.
SMRiP34230. Positions 480-798.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini127 – 417291ABC transmembrane type-1PROSITE-ProRule annotationAdd
BLAST
Domaini472 – 747276ABC transporterPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 ABC transmembrane type-1 domain.PROSITE-ProRule annotation
Contains 1 ABC transporter domain.PROSITE-ProRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

HOGENOMiHOG000206081.
InParanoidiP34230.
KOiK15628.
OMAiMARMYYH.
OrthoDBiEOG7H4F37.

Family and domain databases

Gene3Di3.40.50.300. 2 hits.
InterProiIPR003593. AAA+_ATPase.
IPR011527. ABC1_TM_dom.
IPR003439. ABC_transporter-like.
IPR005283. FA_transporter.
IPR027417. P-loop_NTPase.
IPR031238. Pxa2.
[Graphical view]
PANTHERiPTHR11384:SF13. PTHR11384:SF13. 3 hits.
PfamiPF06472. ABC_membrane_2. 1 hit.
PF00005. ABC_tran. 1 hit.
[Graphical view]
SMARTiSM00382. AAA. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 2 hits.
SSF90123. SSF90123. 1 hit.
TIGRFAMsiTIGR00954. 3a01203. 1 hit.
PROSITEiPS50929. ABC_TM1F. 1 hit.
PS50893. ABC_TRANSPORTER_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P34230-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MISTASAFYQ KHRVNLLRSS YIILLLATLY NSNSSSSNNK TDKKDSESTV
60 70 80 90 100
LENKKIEEGK ETAVDREEDE SSKEELTIVS KHSTDSEDGA IIIDKESKTN
110 120 130 140 150
HKGGERKGKV DFLFKLLLHD KKCLILFITQ AILLNIRTLL SLRVATLDGQ
160 170 180 190 200
LVSTLVRAQY ANFTKILLGK WMILGIPASF INSLISYTTK LCAVTINRKV
210 220 230 240 250
SDFLLSKYLS NHHTFYSVAS AESVSEIQDN LTKDIYTFSM NSSLLLNQLL
260 270 280 290 300
KPMLDLILCS FKLLTSNTSV MGEGTLALGL IVYASNSLLK LIQPNFTRLT
310 320 330 340 350
MASASLESWF RSLHSNLHSS NEEIALLRGQ KRELENVDYS FYRLVLFLNR
360 370 380 390 400
EIKARAIYDV ATAFVIKYTW GAAGLVLCSI PIFFKNKPSE DTLQLKEPGN
410 420 430 440 450
DMTADFITNR RLLVTASSSI GRFVELKRNI QQLRGIRLRL NKFNDLLDAN
460 470 480 490 500
KGDDEKEPRD ERCIVEYDDS RIKFENIPLI TPANQVLVPE LSFDLKHGNH
510 520 530 540 550
LLIIGPNGCG KSSLFRILGG LWPIRATPNK NHQSKLIMPR RTVDRDCAIF
560 570 580 590 600
YLPQRPYMGN RSTFREQIIY PDSIEQFKER YHNDYDLGDA DLIKILQLLD
610 620 630 640 650
LEDLVTENMS LLLAQRTSKN DSQQLSTEDN QSPCAIKVRD AFSIVRNWSE
660 670 680 690 700
ELTIGVQQRL AMARMYYHKP KFAVLDECTS AVAPEMEQRM YENAQNFGIS
710 720 730 740 750
LISVCHRTSL WHFHNYLLKF DGKGGYQFGP FNPKERLCNE EKLLELNAIL
760 770 780 790 800
DQQVPLWERK LKDLTIAKES NIIRKSETNL NLFEKIEDPK TSKSNALFNA
810 820 830 840 850
NKGQRITSPT GQETSKRLPL FSQPSSSASS NLLRNNKSLN KKVKTKKEEG

KER
Length:853
Mass (Da):97,126
Last modified:February 1, 1994 - v1
Checksum:iBDD37FB2A6A220DB
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U93584 Genomic DNA. Translation: AAB51597.1.
X74151 Genomic DNA. Translation: CAA52250.1.
X76133 Genomic DNA. Translation: CAA53736.1.
Z28188 Genomic DNA. Translation: CAA82031.1.
BK006944 Genomic DNA. Translation: DAA08978.1.
PIRiS34682.
RefSeqiNP_012733.1. NM_001179754.1.

Genome annotation databases

EnsemblFungiiYKL188C; YKL188C; YKL188C.
GeneIDi853647.
KEGGisce:YKL188C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U93584 Genomic DNA. Translation: AAB51597.1.
X74151 Genomic DNA. Translation: CAA52250.1.
X76133 Genomic DNA. Translation: CAA53736.1.
Z28188 Genomic DNA. Translation: CAA82031.1.
BK006944 Genomic DNA. Translation: DAA08978.1.
PIRiS34682.
RefSeqiNP_012733.1. NM_001179754.1.

3D structure databases

ProteinModelPortaliP34230.
SMRiP34230. Positions 480-798.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi33934. 76 interactions.
DIPiDIP-741N.
IntActiP34230. 1 interaction.
MINTiMINT-1164061.

Protein family/group databases

TCDBi3.A.1.203.2. the atp-binding cassette (abc) superfamily.
3.A.1.203.6. the atp-binding cassette (abc) superfamily.

Proteomic databases

PeptideAtlasiP34230.
TopDownProteomicsiP34230.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYKL188C; YKL188C; YKL188C.
GeneIDi853647.
KEGGisce:YKL188C.

Organism-specific databases

EuPathDBiFungiDB:YKL188C.
SGDiS000001671. PXA2.

Phylogenomic databases

HOGENOMiHOG000206081.
InParanoidiP34230.
KOiK15628.
OMAiMARMYYH.
OrthoDBiEOG7H4F37.

Enzyme and pathway databases

BioCyciYEAST:G3O-31951-MONOMER.
BRENDAi3.6.3.47. 984.

Miscellaneous databases

NextBioi974551.
PROiP34230.

Family and domain databases

Gene3Di3.40.50.300. 2 hits.
InterProiIPR003593. AAA+_ATPase.
IPR011527. ABC1_TM_dom.
IPR003439. ABC_transporter-like.
IPR005283. FA_transporter.
IPR027417. P-loop_NTPase.
IPR031238. Pxa2.
[Graphical view]
PANTHERiPTHR11384:SF13. PTHR11384:SF13. 3 hits.
PfamiPF06472. ABC_membrane_2. 1 hit.
PF00005. ABC_tran. 1 hit.
[Graphical view]
SMARTiSM00382. AAA. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 2 hits.
SSF90123. SSF90123. 1 hit.
TIGRFAMsiTIGR00954. 3a01203. 1 hit.
PROSITEiPS50929. ABC_TM1F. 1 hit.
PS50893. ABC_TRANSPORTER_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "A Saccharomyces cerevisiae homolog of the human adrenoleukodystrophy transporter is a heterodimer of two half ATP-binding cassette transporters."
    Shani N., Valle D.
    Proc. Natl. Acad. Sci. U.S.A. 93:11901-11906(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "Sequencing and analysis of 51.6 kilobases on the left arm of chromosome XI from Saccharomyces cerevisiae reveals 23 open reading frames including the FAS1 gene."
    Wiemann S., Voss H., Schwager C., Rupp T., Stegemann J., Zimmermann J., Grothues D., Sensen C., Erfle H., Hewitt N., Banrevi A., Ansorge W.
    Yeast 9:1343-1348(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  3. "The yeast YKL741 gene situated on the left arm of chromosome XI codes for a homologue of the human ALD protein."
    Bossier P., Fernandes L., Vilela C., Rodrigues-Pousada C.
    Yeast 10:681-686(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  4. "Complete DNA sequence of yeast chromosome XI."
    Dujon B., Alexandraki D., Andre B., Ansorge W., Baladron V., Ballesta J.P.G., Banrevi A., Bolle P.-A., Bolotin-Fukuhara M., Bossier P., Bou G., Boyer J., Buitrago M.J., Cheret G., Colleaux L., Daignan-Fornier B., del Rey F., Dion C.
    , Domdey H., Duesterhoeft A., Duesterhus S., Entian K.-D., Erfle H., Esteban P.F., Feldmann H., Fernandes L., Fobo G.M., Fritz C., Fukuhara H., Gabel C., Gaillon L., Garcia-Cantalejo J.M., Garcia-Ramirez J.J., Gent M.E., Ghazvini M., Goffeau A., Gonzalez A., Grothues D., Guerreiro P., Hegemann J.H., Hewitt N., Hilger F., Hollenberg C.P., Horaitis O., Indge K.J., Jacquier A., James C.M., Jauniaux J.-C., Jimenez A., Keuchel H., Kirchrath L., Kleine K., Koetter P., Legrain P., Liebl S., Louis E.J., Maia e Silva A., Marck C., Monnier A.-L., Moestl D., Mueller S., Obermaier B., Oliver S.G., Pallier C., Pascolo S., Pfeiffer F., Philippsen P., Planta R.J., Pohl F.M., Pohl T.M., Poehlmann R., Portetelle D., Purnelle B., Puzos V., Ramezani Rad M., Rasmussen S.W., Remacha M.A., Revuelta J.L., Richard G.-F., Rieger M., Rodrigues-Pousada C., Rose M., Rupp T., Santos M.A., Schwager C., Sensen C., Skala J., Soares H., Sor F., Stegemann J., Tettelin H., Thierry A., Tzermia M., Urrestarazu L.A., van Dyck L., van Vliet-Reedijk J.C., Valens M., Vandenbol M., Vilela C., Vissers S., von Wettstein D., Voss H., Wiemann S., Xu G., Zimmermann J., Haasemann M., Becker I., Mewes H.-W.
    Nature 369:371-378(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  5. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  6. "The ABC transporter proteins Pat1 and Pat2 are required for import of long-chain fatty acids into peroxisomes of Saccharomyces cerevisiae."
    Hettema E.H., van Roermund C.W.T., Distel B., van den Berg M., Vilela C., Rodrigues-Pousada C., Wanders R.J.A., Tabak H.F.
    EMBO J. 15:3813-3822(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.

Entry informationi

Entry nameiPXA2_YEAST
AccessioniPrimary (citable) accession number: P34230
Secondary accession number(s): D6VX12
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: February 1, 1994
Last modified: May 11, 2016
This is version 140 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XI
    Yeast (Saccharomyces cerevisiae) chromosome XI: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.