P34230 (PXA2_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Peroxisomal long-chain fatty acid import protein 1

Alternative name(s):
Peroxisomal ABC transporter 2

Gene names

Name:	PXA2
Synonyms:	PAT1
Ordered Locus Names:	YKL188C
ORF Names:	YKL741

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	853 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Involved in the import of activated long-chain fatty acids from the cytosol to the peroxisomal matrix. Ref.6
Subunit structure	Forms an heterodimer with PXA1.
Subcellular location	Peroxisome membrane; Multi-pass membrane protein.
Sequence similarities	Belongs to the ABC transporter superfamily. ABCD family. Peroxisomal fatty acyl CoA transporter (TC 3.A.1.203) subfamily. [View classification] Contains 1 ABC transmembrane type-1 domain. Contains 1 ABC transporter domain.

Ontologies

Keywords
Biological process	Transport
Cellular component	Membrane Peroxisome
Domain	Transmembrane Transmembrane helix
Ligand	ATP-binding Nucleotide-binding
PTM	Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	fatty acid transport Inferred from direct assay. Source: SGD
Cellular component	integral to peroxisomal membrane Inferred from direct assay. Source: SGD
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW ATPase activity, coupled to transmembrane movement of substances Inferred from direct assay. Source: SGD protein binding Inferred from physical interaction Ref.1. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
PXA1	P41909	2	EBI-2464632,EBI-14344

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 853	853	Peroxisomal long-chain fatty acid import protein 1		PRO_0000093315
Regions
	Transmembrane	122 – 142	21	Helical; Potential
	Transmembrane	166 – 186	21	Helical; Potential
	Transmembrane	269 – 289	21	Helical; Potential
	Transmembrane	364 – 384	21	Helical; Potential
	Domain	127 – 417	291	ABC transmembrane type-1
	Domain	472 – 747	276	ABC transporter
	Nucleotide binding	505 – 512	8	ATP Potential
Amino acid modifications
	Glycosylation	33	1	N-linked (GlcNAc...) Potential
	Glycosylation	39	1	N-linked (GlcNAc...) Potential
	Glycosylation	162	1	N-linked (GlcNAc...) Potential
	Glycosylation	230	1	N-linked (GlcNAc...) Potential
	Glycosylation	241	1	N-linked (GlcNAc...) Potential
	Glycosylation	267	1	N-linked (GlcNAc...) Potential
	Glycosylation	295	1	N-linked (GlcNAc...) Potential
	Glycosylation	560	1	N-linked (GlcNAc...) Potential
	Glycosylation	608	1	N-linked (GlcNAc...) Potential
	Glycosylation	620	1	N-linked (GlcNAc...) Potential
	Glycosylation	647	1	N-linked (GlcNAc...) Potential
	Glycosylation	836	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	P34230 [UniParc]. Last modified February 1, 1994. Version 1. Checksum: BDD37FB2A6A220DB	FASTA	853	97,126
	10 20 30 40 50 60 MISTASAFYQ KHRVNLLRSS YIILLLATLY NSNSSSSNNK TDKKDSESTV LENKKIEEGK 70 80 90 100 110 120 ETAVDREEDE SSKEELTIVS KHSTDSEDGA IIIDKESKTN HKGGERKGKV DFLFKLLLHD 130 140 150 160 170 180 KKCLILFITQ AILLNIRTLL SLRVATLDGQ LVSTLVRAQY ANFTKILLGK WMILGIPASF 190 200 210 220 230 240 INSLISYTTK LCAVTINRKV SDFLLSKYLS NHHTFYSVAS AESVSEIQDN LTKDIYTFSM 250 260 270 280 290 300 NSSLLLNQLL KPMLDLILCS FKLLTSNTSV MGEGTLALGL IVYASNSLLK LIQPNFTRLT 310 320 330 340 350 360 MASASLESWF RSLHSNLHSS NEEIALLRGQ KRELENVDYS FYRLVLFLNR EIKARAIYDV 370 380 390 400 410 420 ATAFVIKYTW GAAGLVLCSI PIFFKNKPSE DTLQLKEPGN DMTADFITNR RLLVTASSSI 430 440 450 460 470 480 GRFVELKRNI QQLRGIRLRL NKFNDLLDAN KGDDEKEPRD ERCIVEYDDS RIKFENIPLI 490 500 510 520 530 540 TPANQVLVPE LSFDLKHGNH LLIIGPNGCG KSSLFRILGG LWPIRATPNK NHQSKLIMPR 550 560 570 580 590 600 RTVDRDCAIF YLPQRPYMGN RSTFREQIIY PDSIEQFKER YHNDYDLGDA DLIKILQLLD 610 620 630 640 650 660 LEDLVTENMS LLLAQRTSKN DSQQLSTEDN QSPCAIKVRD AFSIVRNWSE ELTIGVQQRL 670 680 690 700 710 720 AMARMYYHKP KFAVLDECTS AVAPEMEQRM YENAQNFGIS LISVCHRTSL WHFHNYLLKF 730 740 750 760 770 780 DGKGGYQFGP FNPKERLCNE EKLLELNAIL DQQVPLWERK LKDLTIAKES NIIRKSETNL 790 800 810 820 830 840 NLFEKIEDPK TSKSNALFNA NKGQRITSPT GQETSKRLPL FSQPSSSASS NLLRNNKSLN 850 KKVKTKKEEG KER

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"A Saccharomyces cerevisiae homolog of the human adrenoleukodystrophy transporter is a heterodimer of two half ATP-binding cassette transporters." Shani N., Valle D. Proc. Natl. Acad. Sci. U.S.A. 93:11901-11906(1996) [PubMed: 8876235] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Sequencing and analysis of 51.6 kilobases on the left arm of chromosome XI from Saccharomyces cerevisiae reveals 23 open reading frames including the FAS1 gene." Wiemann S., Voss H., Schwager C., Rupp T., Stegemann J., Zimmermann J., Grothues D., Sensen C., Erfle H., Hewitt N., Banrevi A., Ansorge W. Yeast 9:1343-1348(1993) [PubMed: 8154185] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"The yeast YKL741 gene situated on the left arm of chromosome XI codes for a homologue of the human ALD protein." Bossier P., Fernandes L., Vilela C., Rodrigues-Pousada C. Yeast 10:681-686(1994) [PubMed: 7941751] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[4]	"Complete DNA sequence of yeast chromosome XI." Dujon B., Alexandraki D., Andre B., Ansorge W., Baladron V., Ballesta J.P.G., Banrevi A., Bolle P.-A., Bolotin-Fukuhara M., Bossier P., Bou G., Boyer J., Buitrago M.J., Cheret G., Colleaux L., Daignan-Fornier B., del Rey F., Dion C. , Domdey H., Duesterhoeft A., Duesterhus S., Entian K.-D., Erfle H., Esteban P.F., Feldmann H., Fernandes L., Fobo G.M., Fritz C., Fukuhara H., Gabel C., Gaillon L., Garcia-Cantalejo J.M., Garcia-Ramirez J.J., Gent M.E., Ghazvini M., Goffeau A., Gonzalez A., Grothues D., Guerreiro P., Hegemann J.H., Hewitt N., Hilger F., Hollenberg C.P., Horaitis O., Indge K.J., Jacquier A., James C.M., Jauniaux J.-C., Jimenez A., Keuchel H., Kirchrath L., Kleine K., Koetter P., Legrain P., Liebl S., Louis E.J., Maia e Silva A., Marck C., Monnier A.-L., Moestl D., Mueller S., Obermaier B., Oliver S.G., Pallier C., Pascolo S., Pfeiffer F., Philippsen P., Planta R.J., Pohl F.M., Pohl T.M., Poehlmann R., Portetelle D., Purnelle B., Puzos V., Ramezani Rad M., Rasmussen S.W., Remacha M.A., Revuelta J.L., Richard G.-F., Rieger M., Rodrigues-Pousada C., Rose M., Rupp T., Santos M.A., Schwager C., Sensen C., Skala J., Soares H., Sor F., Stegemann J., Tettelin H., Thierry A., Tzermia M., Urrestarazu L.A., van Dyck L., van Vliet-Reedijk J.C., Valens M., Vandenbol M., Vilela C., Vissers S., von Wettstein D., Voss H., Wiemann S., Xu G., Zimmermann J., Haasemann M., Becker I., Mewes H.-W. Nature 369:371-378(1994) [PubMed: 8196765] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[5]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[6]	"The ABC transporter proteins Pat1 and Pat2 are required for import of long-chain fatty acids into peroxisomes of Saccharomyces cerevisiae." Hettema E.H., van Roermund C.W.T., Distel B., van den Berg M., Vilela C., Rodrigues-Pousada C., Wanders R.J.A., Tabak H.F. EMBO J. 15:3813-3822(1996) [PubMed: 8670886] [Abstract] Cited for: FUNCTION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U93584 Genomic DNA. Translation: AAB51597.1. X74151 Genomic DNA. Translation: CAA52250.1. X76133 Genomic DNA. Translation: CAA53736.1. Z28188 Genomic DNA. Translation: CAA82031.1. BK006944 Genomic DNA. Translation: DAA08978.1.
PIR	S34682.
RefSeq	NP_012733.1. NM_001179754.1.
3D structure databases
ProteinModelPortal	P34230.
SMR	P34230. Positions 496-523, 648-739.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-741N.
IntAct	P34230. 1 interaction.
MINT	MINT-1164061.
STRING	P34230.
Protein family/group databases
TCDB	3.A.1.203.2. ATP-binding cassette (ABC) superfamily. 3.A.1.203.6. ATP-binding cassette (ABC) superfamily.
Proteomic databases
PeptideAtlas	P34230.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YKL188C; YKL188C; YKL188C.
GeneID	853647.
KEGG	sce:YKL188C.
NMPDR	fig|4932.3.peg.3710.
Organism-specific databases
CYGD	YKL188c.
SGD	S000001671. PXA2.
Phylogenomic databases
eggNOG	fuNOG06873.
GeneTree	EFGT00050000001687.
HOGENOM	HBG557888.
OMA	LAMARMY.
OrthoDB	EOG4Z39P6.
Gene expression databases
ArrayExpress	P34230.
Genevestigator	P34230.
GermOnline	YKL188C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR010509. ABC_Ald_N. IPR003439. ABC_transporter-like. IPR017940. ABC_transporter_type1. IPR011527. ABC_transptrTM_dom_typ1. IPR003593. ATPase_AAA+_core. IPR005283. FA_transporter. [Graphical view]
KO	K15628.
Pfam	PF06472. ABC_membrane_2. 1 hit. PF00005. ABC_tran. 1 hit. [Graphical view]
SMART	SM00382. AAA. 1 hit. [Graphical view]
SUPFAM	SSF90123. ABC_TM_1. 1 hit.
TIGRFAMs	TIGR00954. 3a01203. 1 hit.
PROSITE	PS50929. ABC_TM1F. 1 hit. PS00211. ABC_TRANSPORTER_1. False negative. PS50893. ABC_TRANSPORTER_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	974551.

Entry information

Entry name

PXA2_YEAST

Accession

Primary (citable) accession number: P34230
Secondary accession number(s): D6VX12

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1994
Last sequence update:	February 1, 1994
Last modified:	January 25, 2012
This is version 103 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program