P34228 (SEF1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Putative transcription factor SEF1

Alternative name(s):
Suppressor of essential function protein 1
Suppressor protein SEF1

Gene names

Name:	SEF1
Ordered Locus Names:	YBL066C
ORF Names:	YBL0501, YBL0526

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	1148 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Putative transcription factor that seems to be involved in the sporulation process. Suppresses the lethal phenotype of RPM2 deletion. Ref.4
Subcellular location	Nucleus Probable.
Sequence similarities	Contains 1 Zn(2)-C6 fungal-type DNA-binding domain.
Sequence caution	The sequence CAA84885.1 differs from that shown. Reason: Sequencing errors.

Ontologies

Keywords
Biological process	Sporulation Transcription Transcription regulation
Cellular component	Nucleus
Ligand	DNA-binding Metal-binding Zinc
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	sporulation resulting in formation of a cellular spore Inferred from electronic annotation. Source: UniProtKB-KW transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	DNA binding Inferred from electronic annotation. Source: UniProtKB-KW sequence-specific DNA binding transcription factor activity Inferred from electronic annotation. Source: InterPro zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1148	1148	Putative transcription factor SEF1		PRO_0000114977
Regions
	DNA binding	57 – 87	31	Zn(2)-C6 fungal-type
	Compositional bias	278 – 322	45	Thr-rich
	Compositional bias	536 – 539	4	Poly-Asn
Amino acid modifications
	Modified residue	163	1	Phosphoserine Ref.5
	Modified residue	165	1	Phosphoserine Ref.5
	Modified residue	263	1	Phosphoserine Ref.5
	Modified residue	328	1	Phosphoserine Ref.5
	Modified residue	845	1	Phosphothreonine Ref.5
	Modified residue	846	1	Phosphothreonine Ref.5
	Modified residue	848	1	Phosphoserine Ref.5
	Modified residue	968	1	Phosphoserine Ref.5
	Modified residue	970	1	Phosphoserine Ref.5

Sequences

	Sequence		Length	Mass (Da)
	P34228 [UniParc]. Last modified November 28, 2006. Version 4. Checksum: 41553B72ED667645	FASTA	1,148	127,992
	10 20 30 40 50 60 MVKDNRDSDQ DQDFSSAHMK RQPEQQQLQQ HQFPSKKQRI SHHDDSHQIN HRPVTSCTHC 70 80 90 100 110 120 RQHKIKCDAS QNFPHPCSRC EKIGLHCEIN PQFRPKKGSQ LQLLRQDVDE IKSKLDTLLA 130 140 150 160 170 180 NDSVFVHLLQ QIPMGNSLLN KLNLHPTPTP GTIIPNPDSS PSSGSPTSSA AQRDSKVSVQ 190 200 210 220 230 240 TYLSREPQLL QANQGSNTNK FKANNEASSH MTLRASSLAQ DSKGLVATEP NKLPPLLNDS 250 260 270 280 290 300 ALPNNSKESL PPALQMAFYK NNSAGNTPNG PFSPIQKTYS PHTTSTTVTT TTNQPPFAAT 310 320 330 340 350 360 SHVATNNNAD RTKTPVVATT TTMPLLPSPH ANVDEFVLGD ISISIEKANR LHHIFVTRYL 370 380 390 400 410 420 PYFPIMYSNN ATELYSQSQL LFWTVMLTAC LSDPEPTMYC KLSSLIKQLA IETCWIRTPR 430 440 450 460 470 480 STHISQALLI LCIWPLPNQK VLDDCSYRFV GLAKSLSYQL GLHRGEFISE FTRTQTSMPN 490 500 510 520 530 540 AEKWRTRTWL GIFFAELCWA SILGLPPTSQ TDYLLEKALS CGDEESEEDN NDSIDNNNND 550 560 570 580 590 600 KRNKKDEPHV ESKYKLPGSF RRLLSLANFQ AKLSHIIGSS TSSPDGLLEP KYRAETLSIL 610 620 630 640 650 660 GKELDLLAKT LNFQSDDTVN IYFLYVKLTV CCFAFLPETP PTDQIPYVTE AYLTATKIVT 670 680 690 700 710 720 LLNNLLETHQ LIELPIYIRQ AATFSALILF KLQLTPLLPD KYFDSARQSV VTIHRLYRNQ 730 740 750 760 770 780 LTAWATSVEN DISRTASMLE KLNFVLIMHP EVFVEEDGII SRMRSHLTGS LFYDLVWCVH 790 800 810 820 830 840 EARRREMDPE YNKQALEKAA KKRKFSSNGI YNGTSSTGGI TDRKLYPLPL YNHISRDDFE 850 860 870 880 890 900 TVTKTTPSGT TVTTLVPTKN ALKQAEKLAK TNNGDSDGSI MEINGIPLSM LGETGSVKFQ 910 920 930 940 950 960 SLFANTSNSN DYNNNRTLLD ASNDISIPSN SIYPVASVPA SNNNPQSTKV DYYSNGPSVI 970 980 990 1000 1010 1020 PDLSMKRSVS TPVNHFPASV PGLRNHPVGN LSNNVTLGID HPIPREHSNL QNVTMNYNNQ 1030 1040 1050 1060 1070 1080 FSNANAIGRS QSSMSHSRTP IASKSNNMTD LHSVVSDPGS SKSTAYPPLS LFSKSNDINS 1090 1100 1110 1120 1130 1140 NKTNQRFSTG TNTVTSSNFQ TIDNENNVKT PGNKLTDFFQ QQSAGWIEGN SSNDDFFGWF DMNMEQGF

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Complete DNA sequence of yeast chromosome II." Feldmann H., Aigle M., Aljinovic G., Andre B., Baclet M.C., Barthe C., Baur A., Becam A.-M., Biteau N., Boles E., Brandt T., Brendel M., Brueckner M., Bussereau F., Christiansen C., Contreras R., Crouzet M., Cziepluch C. , Demolis N., Delaveau T., Doignon F., Domdey H., Duesterhus S., Dubois E., Dujon B., El Bakkoury M., Entian K.-D., Feuermann M., Fiers W., Fobo G.M., Fritz C., Gassenhuber J., Glansdorff N., Goffeau A., Grivell L.A., de Haan M., Hein C., Herbert C.J., Hollenberg C.P., Holmstroem K., Jacq C., Jacquet M., Jauniaux J.-C., Jonniaux J.-L., Kallesoee T., Kiesau P., Kirchrath L., Koetter P., Korol S., Liebl S., Logghe M., Lohan A.J.E., Louis E.J., Li Z.Y., Maat M.J., Mallet L., Mannhaupt G., Messenguy F., Miosga T., Molemans F., Mueller S., Nasr F., Obermaier B., Perea J., Pierard A., Piravandi E., Pohl F.M., Pohl T.M., Potier S., Proft M., Purnelle B., Ramezani Rad M., Rieger M., Rose M., Schaaff-Gerstenschlaeger I., Scherens B., Schwarzlose C., Skala J., Slonimski P.P., Smits P.H.M., Souciet J.-L., Steensma H.Y., Stucka R., Urrestarazu L.A., van der Aart Q.J.M., Van Dyck L., Vassarotti A., Vetter I., Vierendeels F., Vissers S., Wagner G., de Wergifosse P., Wolfe K.H., Zagulski M., Zimmermann F.K., Mewes H.-W., Kleine K. EMBO J. 13:5795-5809(1994) [PubMed: 7813418] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[2]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[3]	"Sequencing and functional analysis of a 32,560 bp segment on the left arm of yeast chromosome II. Identification of 26 open reading frames, including the KIP1 and SEC17 genes." Scherens B., el Bakkoury M., Vierendeels F., Dubois E., Messenguy F. Yeast 9:1355-1371(1993) [PubMed: 8154187] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-789. Strain: ATCC 204508 / S288c.
[4]	"Kluyveromyces lactis SEF1 and its Saccharomyces cerevisiae homologue bypass the unknown essential function, but not the mitochondrial RNase P function, of the S. cerevisiae RPM2 gene." Groom K.R., Heyman H.C., Steffen M.C., Hawkins L., Martin N.C. Yeast 14:77-87(1998) [PubMed: 9483797] [Abstract] Cited for: FUNCTION.
[5]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed: 18407956] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-163; SER-165; SER-263; SER-328; THR-845; THR-846; SER-848; SER-968 AND SER-970, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Z35827 Genomic DNA. Translation: CAA84885.1. Sequence problems. Z23261 Genomic DNA. Translation: CAA80783.1. BK006936 Genomic DNA. Translation: DAA07055.1.
PIR	S45801.
RefSeq	NP_009487.2. NM_001178306.1.
3D structure databases
ProteinModelPortal	P34228.
SMR	P34228. Positions 52-118.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-2559N.
IntAct	P34228. 5 interactions.
MINT	MINT-426958.
STRING	P34228.
Proteomic databases
PeptideAtlas	P34228.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YBL066C; YBL066C; YBL066C.
GeneID	852214.
KEGG	sce:YBL066C.
NMPDR	fig|4932.3.peg.176.
Organism-specific databases
CYGD	YBL066c.
SGD	S000000162. SEF1.
Phylogenomic databases
eggNOG	fuNOG07960.
GeneTree	EFGT00050000001975.
HOGENOM	HBG397880.
OMA	WIEGNSS.
OrthoDB	EOG4G1QRG.
Gene expression databases
ArrayExpress	P34228.
Genevestigator	P34228.
GermOnline	YBL066C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR007219. Transcription_factor_fun. IPR001138. Zn2-C6_fun-type_DNA-bd. [Graphical view]
Gene3D	G3DSA:4.10.240.10. Zn2-C6_fun-type_DNA-bd. 1 hit.
Pfam	PF04082. Fungal_trans. 1 hit. PF00172. Zn_clus. 1 hit. [Graphical view]
SMART	SM00906. Fungal_trans. 1 hit. SM00066. GAL4. 1 hit. [Graphical view]
SUPFAM	SSF57701. Fungi_TrN. 1 hit.
PROSITE	PS00463. ZN2_CY6_FUNGAL_1. 1 hit. PS50048. ZN2_CY6_FUNGAL_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	970721.

Entry information

Entry name

SEF1_YEAST

Accession

Primary (citable) accession number: P34228
Secondary accession number(s): D6VPT5

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1994
Last sequence update:	November 28, 2006
Last modified:	December 14, 2011
This is version 98 of the entry and version 4 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program