Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

P34227

- PRX1_YEAST

UniProt

P34227 - PRX1_YEAST

Protein

Mitochondrial peroxiredoxin PRX1

Gene

PRX1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 122 (01 Oct 2014)
      Sequence version 1 (01 Feb 1994)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Has a thioredoxin peroxidase activity with a role in reduction of hydroperoxides.1 Publication

    Catalytic activityi

    2 R'-SH + ROOH = R'-S-S-R' + H2O + ROH.

    pH dependencei

    Optimum pH is 6.5.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei91 – 911By similarity

    GO - Molecular functioni

    1. thioredoxin peroxidase activity Source: SGD

    GO - Biological processi

    1. cell redox homeostasis Source: SGD
    2. cellular response to oxidative stress Source: SGD
    3. response to cadmium ion Source: SGD

    Keywords - Molecular functioni

    Antioxidant, Oxidoreductase, Peroxidase

    Enzyme and pathway databases

    BioCyciRETL1328306-WGS:GSTH-1836-MONOMER.
    YEAST:G3O-28961-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Mitochondrial peroxiredoxin PRX1 (EC:1.11.1.15)
    Alternative name(s):
    1-Cys PRX
    Mitochondrial thiol peroxidase
    Short name:
    mTPx
    Thioredoxin reductase
    Gene namesi
    Name:PRX1
    Ordered Locus Names:YBL064C
    ORF Names:YBL0503, YBL0524
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome II

    Organism-specific databases

    CYGDiYBL064c.
    SGDiS000000160. PRX1.

    Subcellular locationi

    Mitochondrion 2 Publications

    GO - Cellular componenti

    1. mitochondrion Source: SGD

    Keywords - Cellular componenti

    Mitochondrion

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi91 – 911C → S: No activity. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 261261Mitochondrial peroxiredoxin PRX1PRO_0000135151Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei53 – 531Phosphoserine1 Publication

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiP34227.
    PaxDbiP34227.
    PeptideAtlasiP34227.

    2D gel databases

    UCD-2DPAGEP34227.

    Expressioni

    Gene expression databases

    GenevestigatoriP34227.

    Interactioni

    Protein-protein interaction databases

    BioGridi32635. 48 interactions.
    DIPiDIP-6412N.
    IntActiP34227. 11 interactions.
    MINTiMINT-642862.
    STRINGi4932.YBL064C.

    Structurei

    3D structure databases

    ProteinModelPortaliP34227.
    SMRiP34227. Positions 56-258.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini49 – 212164ThioredoxinPROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the AhpC/TSA family.Curated
    Contains 1 thioredoxin domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Redox-active center

    Phylogenomic databases

    eggNOGiCOG0450.
    GeneTreeiENSGT00550000074794.
    HOGENOMiHOG000022346.
    KOiK03386.
    OMAiKAMLVYP.
    OrthoDBiEOG7RBZM1.

    Family and domain databases

    Gene3Di3.40.30.10. 1 hit.
    InterProiIPR000866. AhpC/TSA.
    IPR024706. Peroxiredoxin_AhpC-typ.
    IPR019479. Peroxiredoxin_C.
    IPR012336. Thioredoxin-like_fold.
    [Graphical view]
    PfamiPF10417. 1-cysPrx_C. 1 hit.
    PF00578. AhpC-TSA. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000239. AHPC. 1 hit.
    SUPFAMiSSF52833. SSF52833. 1 hit.
    PROSITEiPS51352. THIOREDOXIN_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P34227-1 [UniParc]FASTAAdd to Basket

    « Hide

    MFSRICSAQL KRTAWTLPKQ AHLQSQTIKT FATAPILCKQ FKQSDQPRLR    50
    INSDAPNFDA DTTVGKINFY DYLGDSWGVL FSHPADFTPV CTTEVSAFAK 100
    LKPEFDKRNV KLIGLSVEDV ESHEKWIQDI KEIAKVKNVG FPIIGDTFRN 150
    VAFLYDMVDA EGFKNINDGS LKTVRSVFVI DPKKKIRLIF TYPSTVGRNT 200
    SEVLRVIDAL QLTDKEGVVT PINWQPADDV IIPPSVSNDE AKAKFGQFNE 250
    IKPYLRFTKS K 261
    Length:261
    Mass (Da):29,496
    Last modified:February 1, 1994 - v1
    Checksum:iF611A9ED85E4681A
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z23261 Genomic DNA. Translation: CAA80784.1.
    Z35825 Genomic DNA. Translation: CAA84884.1.
    BK006936 Genomic DNA. Translation: DAA07056.1.
    PIRiS39825.
    RefSeqiNP_009489.1. NM_001178304.1.

    Genome annotation databases

    EnsemblFungiiYBL064C; YBL064C; YBL064C.
    GeneIDi852215.
    KEGGisce:YBL064C.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z23261 Genomic DNA. Translation: CAA80784.1 .
    Z35825 Genomic DNA. Translation: CAA84884.1 .
    BK006936 Genomic DNA. Translation: DAA07056.1 .
    PIRi S39825.
    RefSeqi NP_009489.1. NM_001178304.1.

    3D structure databases

    ProteinModelPortali P34227.
    SMRi P34227. Positions 56-258.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 32635. 48 interactions.
    DIPi DIP-6412N.
    IntActi P34227. 11 interactions.
    MINTi MINT-642862.
    STRINGi 4932.YBL064C.

    2D gel databases

    UCD-2DPAGE P34227.

    Proteomic databases

    MaxQBi P34227.
    PaxDbi P34227.
    PeptideAtlasi P34227.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YBL064C ; YBL064C ; YBL064C .
    GeneIDi 852215.
    KEGGi sce:YBL064C.

    Organism-specific databases

    CYGDi YBL064c.
    SGDi S000000160. PRX1.

    Phylogenomic databases

    eggNOGi COG0450.
    GeneTreei ENSGT00550000074794.
    HOGENOMi HOG000022346.
    KOi K03386.
    OMAi KAMLVYP.
    OrthoDBi EOG7RBZM1.

    Enzyme and pathway databases

    BioCyci RETL1328306-WGS:GSTH-1836-MONOMER.
    YEAST:G3O-28961-MONOMER.

    Miscellaneous databases

    NextBioi 970724.
    PROi P34227.

    Gene expression databases

    Genevestigatori P34227.

    Family and domain databases

    Gene3Di 3.40.30.10. 1 hit.
    InterProi IPR000866. AhpC/TSA.
    IPR024706. Peroxiredoxin_AhpC-typ.
    IPR019479. Peroxiredoxin_C.
    IPR012336. Thioredoxin-like_fold.
    [Graphical view ]
    Pfami PF10417. 1-cysPrx_C. 1 hit.
    PF00578. AhpC-TSA. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF000239. AHPC. 1 hit.
    SUPFAMi SSF52833. SSF52833. 1 hit.
    PROSITEi PS51352. THIOREDOXIN_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Sequencing and functional analysis of a 32,560 bp segment on the left arm of yeast chromosome II. Identification of 26 open reading frames, including the KIP1 and SEC17 genes."
      Scherens B., el Bakkoury M., Vierendeels F., Dubois E., Messenguy F.
      Yeast 9:1355-1371(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    2. "Complete DNA sequence of yeast chromosome II."
      Feldmann H., Aigle M., Aljinovic G., Andre B., Baclet M.C., Barthe C., Baur A., Becam A.-M., Biteau N., Boles E., Brandt T., Brendel M., Brueckner M., Bussereau F., Christiansen C., Contreras R., Crouzet M., Cziepluch C.
      , Demolis N., Delaveau T., Doignon F., Domdey H., Duesterhus S., Dubois E., Dujon B., El Bakkoury M., Entian K.-D., Feuermann M., Fiers W., Fobo G.M., Fritz C., Gassenhuber J., Glansdorff N., Goffeau A., Grivell L.A., de Haan M., Hein C., Herbert C.J., Hollenberg C.P., Holmstroem K., Jacq C., Jacquet M., Jauniaux J.-C., Jonniaux J.-L., Kallesoee T., Kiesau P., Kirchrath L., Koetter P., Korol S., Liebl S., Logghe M., Lohan A.J.E., Louis E.J., Li Z.Y., Maat M.J., Mallet L., Mannhaupt G., Messenguy F., Miosga T., Molemans F., Mueller S., Nasr F., Obermaier B., Perea J., Pierard A., Piravandi E., Pohl F.M., Pohl T.M., Potier S., Proft M., Purnelle B., Ramezani Rad M., Rieger M., Rose M., Schaaff-Gerstenschlaeger I., Scherens B., Schwarzlose C., Skala J., Slonimski P.P., Smits P.H.M., Souciet J.-L., Steensma H.Y., Stucka R., Urrestarazu L.A., van der Aart Q.J.M., Van Dyck L., Vassarotti A., Vetter I., Vierendeels F., Vissers S., Wagner G., de Wergifosse P., Wolfe K.H., Zagulski M., Zimmermann F.K., Mewes H.-W., Kleine K.
      EMBO J. 13:5795-5809(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    3. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    4. "Distinct physiological functions of thiol peroxidase isoenzymes in Saccharomyces cerevisiae."
      Park S.G., Cha M.-K., Jeong W., Kim I.-H.
      J. Biol. Chem. 275:5723-5732(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: CHARACTERIZATION, MUTAGENESIS OF CYS-91.
    5. "Mitochondria of Saccharomyces cerevisiae contain one-conserved cysteine type peroxiredoxin with thioredoxin peroxidase activity."
      Pedrajas J.R., Miranda-Vizuete A., Javanmardy N., Gustafsson J.A., Spyrou G.
      J. Biol. Chem. 275:16296-16301(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, SUBCELLULAR LOCATION.
    6. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    7. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
      Strain: ATCC 76625 / YPH499.
    8. "Profiling phosphoproteins of yeast mitochondria reveals a role of phosphorylation in assembly of the ATP synthase."
      Reinders J., Wagner K., Zahedi R.P., Stojanovski D., Eyrich B., van der Laan M., Rehling P., Sickmann A., Pfanner N., Meisinger C.
      Mol. Cell. Proteomics 6:1896-1906(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-53, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Strain: ATCC 76625 / YPH499.

    Entry informationi

    Entry nameiPRX1_YEAST
    AccessioniPrimary (citable) accession number: P34227
    Secondary accession number(s): D6VPT6
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1994
    Last sequence update: February 1, 1994
    Last modified: October 1, 2014
    This is version 122 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 4510 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    3. Yeast chromosome II
      Yeast (Saccharomyces cerevisiae) chromosome II: entries and gene names

    External Data

    Dasty 3