Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Mitochondrial peroxiredoxin PRX1

Gene

PRX1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Has a thioredoxin peroxidase activity with a role in reduction of hydroperoxides.1 Publication

Catalytic activityi

2 R'-SH + ROOH = R'-S-S-R' + H2O + ROH.

pH dependencei

Optimum pH is 6.5.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei91 – 911By similarity

GO - Molecular functioni

  • thioredoxin peroxidase activity Source: SGD

GO - Biological processi

  • cell redox homeostasis Source: SGD
  • cellular response to oxidative stress Source: SGD
  • response to cadmium ion Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Antioxidant, Oxidoreductase, Peroxidase

Enzyme and pathway databases

BioCyciRETL1328306-WGS:GSTH-1836-MONOMER.
YEAST:G3O-28961-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Mitochondrial peroxiredoxin PRX1 (EC:1.11.1.15)
Alternative name(s):
1-Cys PRX
Mitochondrial thiol peroxidase
Short name:
mTPx
Thioredoxin reductase
Gene namesi
Name:PRX1
Ordered Locus Names:YBL064C
ORF Names:YBL0503, YBL0524
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311 Componenti: Chromosome II

Organism-specific databases

CYGDiYBL064c.
EuPathDBiFungiDB:YBL064C.
SGDiS000000160. PRX1.

Subcellular locationi

GO - Cellular componenti

  • mitochondrion Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi91 – 911C → S: No activity. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 261261Mitochondrial peroxiredoxin PRX1PRO_0000135151Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei53 – 531Phosphoserine1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP34227.
PaxDbiP34227.
PeptideAtlasiP34227.

2D gel databases

UCD-2DPAGEP34227.

Interactioni

Protein-protein interaction databases

BioGridi32635. 51 interactions.
DIPiDIP-6412N.
IntActiP34227. 11 interactions.
MINTiMINT-642862.

Structurei

3D structure databases

ProteinModelPortaliP34227.
SMRiP34227. Positions 56-258.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini49 – 212164ThioredoxinPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the AhpC/TSA family.Curated
Contains 1 thioredoxin domain.PROSITE-ProRule annotation

Keywords - Domaini

Redox-active center

Phylogenomic databases

eggNOGiCOG0450.
GeneTreeiENSGT00550000074794.
HOGENOMiHOG000022346.
InParanoidiP34227.
KOiK03386.
OMAiQEDQPRI.
OrthoDBiEOG7RBZM1.

Family and domain databases

Gene3Di3.40.30.10. 1 hit.
InterProiIPR000866. AhpC/TSA.
IPR024706. Peroxiredoxin_AhpC-typ.
IPR019479. Peroxiredoxin_C.
IPR012336. Thioredoxin-like_fold.
[Graphical view]
PfamiPF10417. 1-cysPrx_C. 1 hit.
PF00578. AhpC-TSA. 1 hit.
[Graphical view]
PIRSFiPIRSF000239. AHPC. 1 hit.
SUPFAMiSSF52833. SSF52833. 1 hit.
PROSITEiPS51352. THIOREDOXIN_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P34227-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MFSRICSAQL KRTAWTLPKQ AHLQSQTIKT FATAPILCKQ FKQSDQPRLR
60 70 80 90 100
INSDAPNFDA DTTVGKINFY DYLGDSWGVL FSHPADFTPV CTTEVSAFAK
110 120 130 140 150
LKPEFDKRNV KLIGLSVEDV ESHEKWIQDI KEIAKVKNVG FPIIGDTFRN
160 170 180 190 200
VAFLYDMVDA EGFKNINDGS LKTVRSVFVI DPKKKIRLIF TYPSTVGRNT
210 220 230 240 250
SEVLRVIDAL QLTDKEGVVT PINWQPADDV IIPPSVSNDE AKAKFGQFNE
260
IKPYLRFTKS K
Length:261
Mass (Da):29,496
Last modified:February 1, 1994 - v1
Checksum:iF611A9ED85E4681A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z23261 Genomic DNA. Translation: CAA80784.1.
Z35825 Genomic DNA. Translation: CAA84884.1.
BK006936 Genomic DNA. Translation: DAA07056.1.
PIRiS39825.
RefSeqiNP_009489.1. NM_001178304.1.

Genome annotation databases

EnsemblFungiiYBL064C; YBL064C; YBL064C.
GeneIDi852215.
KEGGisce:YBL064C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z23261 Genomic DNA. Translation: CAA80784.1.
Z35825 Genomic DNA. Translation: CAA84884.1.
BK006936 Genomic DNA. Translation: DAA07056.1.
PIRiS39825.
RefSeqiNP_009489.1. NM_001178304.1.

3D structure databases

ProteinModelPortaliP34227.
SMRiP34227. Positions 56-258.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi32635. 51 interactions.
DIPiDIP-6412N.
IntActiP34227. 11 interactions.
MINTiMINT-642862.

2D gel databases

UCD-2DPAGEP34227.

Proteomic databases

MaxQBiP34227.
PaxDbiP34227.
PeptideAtlasiP34227.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYBL064C; YBL064C; YBL064C.
GeneIDi852215.
KEGGisce:YBL064C.

Organism-specific databases

CYGDiYBL064c.
EuPathDBiFungiDB:YBL064C.
SGDiS000000160. PRX1.

Phylogenomic databases

eggNOGiCOG0450.
GeneTreeiENSGT00550000074794.
HOGENOMiHOG000022346.
InParanoidiP34227.
KOiK03386.
OMAiQEDQPRI.
OrthoDBiEOG7RBZM1.

Enzyme and pathway databases

BioCyciRETL1328306-WGS:GSTH-1836-MONOMER.
YEAST:G3O-28961-MONOMER.

Miscellaneous databases

NextBioi970724.
PROiP34227.

Family and domain databases

Gene3Di3.40.30.10. 1 hit.
InterProiIPR000866. AhpC/TSA.
IPR024706. Peroxiredoxin_AhpC-typ.
IPR019479. Peroxiredoxin_C.
IPR012336. Thioredoxin-like_fold.
[Graphical view]
PfamiPF10417. 1-cysPrx_C. 1 hit.
PF00578. AhpC-TSA. 1 hit.
[Graphical view]
PIRSFiPIRSF000239. AHPC. 1 hit.
SUPFAMiSSF52833. SSF52833. 1 hit.
PROSITEiPS51352. THIOREDOXIN_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Sequencing and functional analysis of a 32,560 bp segment on the left arm of yeast chromosome II. Identification of 26 open reading frames, including the KIP1 and SEC17 genes."
    Scherens B., el Bakkoury M., Vierendeels F., Dubois E., Messenguy F.
    Yeast 9:1355-1371(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  2. "Complete DNA sequence of yeast chromosome II."
    Feldmann H., Aigle M., Aljinovic G., Andre B., Baclet M.C., Barthe C., Baur A., Becam A.-M., Biteau N., Boles E., Brandt T., Brendel M., Brueckner M., Bussereau F., Christiansen C., Contreras R., Crouzet M., Cziepluch C.
    , Demolis N., Delaveau T., Doignon F., Domdey H., Duesterhus S., Dubois E., Dujon B., El Bakkoury M., Entian K.-D., Feuermann M., Fiers W., Fobo G.M., Fritz C., Gassenhuber J., Glansdorff N., Goffeau A., Grivell L.A., de Haan M., Hein C., Herbert C.J., Hollenberg C.P., Holmstroem K., Jacq C., Jacquet M., Jauniaux J.-C., Jonniaux J.-L., Kallesoee T., Kiesau P., Kirchrath L., Koetter P., Korol S., Liebl S., Logghe M., Lohan A.J.E., Louis E.J., Li Z.Y., Maat M.J., Mallet L., Mannhaupt G., Messenguy F., Miosga T., Molemans F., Mueller S., Nasr F., Obermaier B., Perea J., Pierard A., Piravandi E., Pohl F.M., Pohl T.M., Potier S., Proft M., Purnelle B., Ramezani Rad M., Rieger M., Rose M., Schaaff-Gerstenschlaeger I., Scherens B., Schwarzlose C., Skala J., Slonimski P.P., Smits P.H.M., Souciet J.-L., Steensma H.Y., Stucka R., Urrestarazu L.A., van der Aart Q.J.M., Van Dyck L., Vassarotti A., Vetter I., Vierendeels F., Vissers S., Wagner G., de Wergifosse P., Wolfe K.H., Zagulski M., Zimmermann F.K., Mewes H.-W., Kleine K.
    EMBO J. 13:5795-5809(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  4. "Distinct physiological functions of thiol peroxidase isoenzymes in Saccharomyces cerevisiae."
    Park S.G., Cha M.-K., Jeong W., Kim I.-H.
    J. Biol. Chem. 275:5723-5732(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: CHARACTERIZATION, MUTAGENESIS OF CYS-91.
  5. "Mitochondria of Saccharomyces cerevisiae contain one-conserved cysteine type peroxiredoxin with thioredoxin peroxidase activity."
    Pedrajas J.R., Miranda-Vizuete A., Javanmardy N., Gustafsson J.A., Spyrou G.
    J. Biol. Chem. 275:16296-16301(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION.
  6. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  7. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
    Strain: ATCC 76625 / YPH499.
  8. "Profiling phosphoproteins of yeast mitochondria reveals a role of phosphorylation in assembly of the ATP synthase."
    Reinders J., Wagner K., Zahedi R.P., Stojanovski D., Eyrich B., van der Laan M., Rehling P., Sickmann A., Pfanner N., Meisinger C.
    Mol. Cell. Proteomics 6:1896-1906(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-53, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Strain: ATCC 76625 / YPH499.

Entry informationi

Entry nameiPRX1_YEAST
AccessioniPrimary (citable) accession number: P34227
Secondary accession number(s): D6VPT6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: February 1, 1994
Last modified: July 22, 2015
This is version 128 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 4510 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome II
    Yeast (Saccharomyces cerevisiae) chromosome II: entries and gene names

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.