P34226 (SKT5_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Protein SKT5

Gene names

Name:	SKT5
Synonyms:	CAL2, CHS4, CSD4
Ordered Locus Names:	YBL061C
ORF Names:	YBL0506, YBL0519

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	696 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Possible role in protoplast regeneration and killer toxin of K.lactis (PGKL) resistance.
Subunit structure	May interact with CHS3 and seems to be an adaptor (along with BNI4) to link CHS3 to septins. Ref.6
Subcellular location	Cell membrane; Lipid-anchor; Cytoplasmic side Potential Ref.6.
Miscellaneous	Present with 2580 molecules/cell in log phase SD medium. Ref.7
Sequence similarities	Belongs to the SKT5 family. Contains 7 Sel1-like repeats.
Caution	It is uncertain whether Met-1 or Met-15 is the initiator.
Sequence caution	The sequence AAC60564.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Cellular component	Cell membrane Membrane
Domain	Repeat
PTM	Lipoprotein Methylation Phosphoprotein Prenylation
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	cytokinesis Traceable author statement. Source: SGD fungal-type cell wall chitin biosynthetic process Inferred from mutant phenotype. Source: SGD response to osmotic stress Traceable author statement. Source: SGD
Cellular component	cellular bud neck Inferred from direct assay Ref.6. Source: SGD incipient cellular bud site Inferred from direct assay. Source: SGD plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	binding Inferred from electronic annotation. Source: InterPro enzyme activator activity Inferred from mutant phenotype. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 693	693	Protein SKT5		PRO_0000071940
	Propeptide	694 – 696	3	Removed in mature form By similarity		PRO_0000396725
Regions
	Repeat	271 – 306	36	Sel1-like 1
	Repeat	307 – 342	36	Sel1-like 2
	Repeat	343 – 382	40	Sel1-like 3
	Repeat	386 – 423	38	Sel1-like 4
	Repeat	424 – 460	37	Sel1-like 5
	Repeat	461 – 498	38	Sel1-like 6
	Repeat	499 – 534	36	Sel1-like 7
Amino acid modifications
	Modified residue	32	1	Phosphoserine Ref.9
	Modified residue	107	1	Phosphoserine Ref.11
	Modified residue	136	1	Phosphoserine Ref.11
	Modified residue	148	1	Phosphoserine Ref.11
	Modified residue	171	1	Phosphoserine Ref.11
	Modified residue	561	1	Phosphoserine Ref.8
	Modified residue	588	1	Phosphoserine Ref.11
	Modified residue	624	1	Phosphothreonine Ref.10
	Modified residue	693	1	Cysteine methyl ester By similarity
	Lipidation	693	1	S-farnesyl cysteine By similarity
Experimental info
	Sequence conflict	350	1	G → E in AAC60564. Ref.4
	Sequence conflict	643	1	Q → T in AAC60564. Ref.4

Sequences

	Sequence		Length	Mass (Da)
	P34226 [UniParc]. Last modified February 1, 1994. Version 1. Checksum: D50DE825E175D165	FASTA	696	77,066
	10 20 30 40 50 60 MASSPQVHPY KKHLMQSQHI NFDNRGLQFQ NSSLKVGQDF SDNKENRENR DNEDFSTADL 70 80 90 100 110 120 PKRSANQPLI NEHLRAASVP LLSNDIGNSQ EEDFVPVPPP QLHLNNSNNT SLSSLGSTPT 130 140 150 160 170 180 NSPSPGALRQ TNSSTSLTKE QIKKRTRSVD LSHMYLLNGS SDTQLTATNE SVADLSHQMI 190 200 210 220 230 240 SRYLGGKNNT SLVPRLKTIE MYRQNVKKSK DPEVLFQYAQ YMLQTALTIE SSNALVQDSD 250 260 270 280 290 300 KEGNVSQSDL KLQFLKEAQS YLKKLSIKGY SDAQYLLADG YSSGAFGKIE NKEAFVLFQA 310 320 330 340 350 360 AAKHGHIESA YRASHCLEEG LGTTRDSRKS VNFLKFAASR NHPSAMYKLG LYSFYGRMGL 370 380 390 400 410 420 PTDVNTKLNG VKWLSRAAAR ANELTAAAPY ELAKIYHEGF LDVVIPDEKY AMELYIQAAS 430 440 450 460 470 480 LGHVPSATLL AQIYETGNDT VGQDTSLSVH YYTQAALKGD SVAMLGLCAW YLLGAEPAFE 490 500 510 520 530 540 KDENEAFQWA LRAANAGLPK AQFTLGYFYE HGKGCDRNME YAWKWYEKAA GNEDKRAINK 550 560 570 580 590 600 LRSRDGGLAS IGKKQHKKNK SISTLNLFST VDSQTSNVGS NSRVSSKSET FFTGNPKRDR 610 620 630 640 650 660 EPQGLQINMN SNTNRNGIKT GSDTSIRKSS SSAKGMSREV AEQSMAAKQE VSLSNMGSSN 670 680 690 MIRKDFPAVK TESKKPTSLK NKKDKQGKKK KDCVIM

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequencing and functional analysis of a 32,560 bp segment on the left arm of yeast chromosome II. Identification of 26 open reading frames, including the KIP1 and SEC17 genes." Scherens B., el Bakkoury M., Vierendeels F., Dubois E., Messenguy F. Yeast 9:1355-1371(1993) [PubMed: 8154187] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[2]	"Complete DNA sequence of yeast chromosome II." Feldmann H., Aigle M., Aljinovic G., Andre B., Baclet M.C., Barthe C., Baur A., Becam A.-M., Biteau N., Boles E., Brandt T., Brendel M., Brueckner M., Bussereau F., Christiansen C., Contreras R., Crouzet M., Cziepluch C. , Demolis N., Delaveau T., Doignon F., Domdey H., Duesterhus S., Dubois E., Dujon B., El Bakkoury M., Entian K.-D., Feuermann M., Fiers W., Fobo G.M., Fritz C., Gassenhuber J., Glansdorff N., Goffeau A., Grivell L.A., de Haan M., Hein C., Herbert C.J., Hollenberg C.P., Holmstroem K., Jacq C., Jacquet M., Jauniaux J.-C., Jonniaux J.-L., Kallesoee T., Kiesau P., Kirchrath L., Koetter P., Korol S., Liebl S., Logghe M., Lohan A.J.E., Louis E.J., Li Z.Y., Maat M.J., Mallet L., Mannhaupt G., Messenguy F., Miosga T., Molemans F., Mueller S., Nasr F., Obermaier B., Perea J., Pierard A., Piravandi E., Pohl F.M., Pohl T.M., Potier S., Proft M., Purnelle B., Ramezani Rad M., Rieger M., Rose M., Schaaff-Gerstenschlaeger I., Scherens B., Schwarzlose C., Skala J., Slonimski P.P., Smits P.H.M., Souciet J.-L., Steensma H.Y., Stucka R., Urrestarazu L.A., van der Aart Q.J.M., Van Dyck L., Vassarotti A., Vetter I., Vierendeels F., Vissers S., Wagner G., de Wergifosse P., Wolfe K.H., Zagulski M., Zimmermann F.K., Mewes H.-W., Kleine K. EMBO J. 13:5795-5809(1994) [PubMed: 7813418] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[3]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[4]	"A mutant allele skt5 affecting protoplast regeneration and killer toxin resistance has double mutations in its wild-type structural gene in Saccharomyces cerevisiae." Kawamoto S., Sasaki T., Itahashi S., Hatsuyama Y., Ohno T. Biosci. Biotechnol. Biochem. 57:1391-1393(1993) [PubMed: 7764021] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-678. Strain: KR13.
[5]	"Cloning and characterization of SKT5, a Saccharomyces cerevisiae gene that affects protoplast regeneration and resistance to killer toxin of Kluyveromyces lactis." Kawamoto S., Nomura M., Ohno T. J. Ferment. Bioeng. 74:199-208(1992) Cited for: PRELIMINARY PARTIAL NUCLEOTIDE SEQUENCE.
[6]	"A septin-based hierarchy of proteins required for localized deposition of chitin in the Saccharomyces cerevisiae cell wall." DeMarini D.J., Adams A.E., Fares H., De Virgilio C., Valle G., Chuang J.S., Pringle J.R. J. Cell Biol. 139:75-93(1997) [PubMed: 9314530] [Abstract] Cited for: SUBUNIT, SUBCELLULAR LOCATION.
[7]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[8]	"Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae." Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P. J. Proteome Res. 6:1190-1197(2007) [PubMed: 17330950] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-561, MASS SPECTROMETRY. Strain: ADR376.
[9]	"Analysis of phosphorylation sites on proteins from Saccharomyces cerevisiae by electron transfer dissociation (ETD) mass spectrometry." Chi A., Huttenhower C., Geer L.Y., Coon J.J., Syka J.E.P., Bai D.L., Shabanowitz J., Burke D.J., Troyanskaya O.G., Hunt D.F. Proc. Natl. Acad. Sci. U.S.A. 104:2193-2198(2007) [PubMed: 17287358] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-32, MASS SPECTROMETRY.
[10]	"Proteome-wide identification of in vivo targets of DNA damage checkpoint kinases." Smolka M.B., Albuquerque C.P., Chen S.H., Zhou H. Proc. Natl. Acad. Sci. U.S.A. 104:10364-10369(2007) [PubMed: 17563356] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-624, MASS SPECTROMETRY.
[11]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed: 18407956] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-107; SER-136; SER-148; SER-171 AND SER-588, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Z23261 Genomic DNA. Translation: CAA80786.1. Z35823 Genomic DNA. Translation: CAA84882.1. S65415 Genomic DNA. Translation: AAC60564.1. Different initiation. BK006936 Genomic DNA. Translation: DAA07058.1.
PIR	S39827.
RefSeq	NP_009492.1. NM_001178301.1.
3D structure databases
ProteinModelPortal	P34226.
SMR	P34226. Positions 254-688.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-2481N.
IntAct	P34226. 15 interactions.
MINT	MINT-584159.
STRING	P34226.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YBL061C; YBL061C; YBL061C.
GeneID	852218.
KEGG	sce:YBL061C.
NMPDR	fig|4932.3.peg.181.
Organism-specific databases
CYGD	YBL061c.
SGD	S000000157. SKT5.
Phylogenomic databases
eggNOG	fuNOG06114.
GeneTree	EFGT00050000000230.
HOGENOM	HBG204244.
OMA	WALRAAN.
OrthoDB	EOG4XWK6F.
Gene expression databases
ArrayExpress	P34226.
Genevestigator	P34226.
GermOnline	YBL061C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR006597. Sel1-like. IPR011990. TPR-like_helical. [Graphical view]
Gene3D	G3DSA:1.25.40.10. TPR-like_helical. 1 hit.
KO	K07126.
Pfam	PF08238. Sel1. 7 hits. [Graphical view]
SMART	SM00671. SEL1. 7 hits. [Graphical view]
ProtoNet	Search...
Other
NextBio	970730.

Entry information

Entry name

SKT5_YEAST

Accession

Primary (citable) accession number: P34226
Secondary accession number(s): D6VPT8, Q02215

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1994
Last sequence update:	February 1, 1994
Last modified:	January 25, 2012
This is version 107 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program