Reviewed, UniProtKB/Swiss-Prot P34225 (YBG0_YEAST)

Names and origin

Protein names

Recommended name:
    PH and SEC7 domain-containing protein YBL060W

Gene names

Ordered Locus Names:	YBL060W
ORF Names:	YBL0507, YBL0517

Organism

Saccharomyces cerevisiae (Baker's yeast) [Complete proteome]

Taxonomic identifier

4932 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	687 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Sequence similarities

Contains 1 PH domain. Contains 1 SEC7 domain.

Ontologies

Keywords
PTM	Phosphoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	actin cortical patch localization Inferred from mutant phenotype. Source: SGD protein localization Inferred from mutant phenotype. Source: SGD regulation of ARF protein signal transduction Inferred from electronic annotation. Source: InterPro
Cellular component	cellular bud neck Inferred from direct assay. Source: SGD cellular bud tip Inferred from direct assay. Source: SGD cytoplasm Inferred from direct assay. Source: SGD
Molecular function	ARF guanyl-nucleotide exchange factor activity Inferred from direct assay. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 687	687	PH and SEC7 domain-containing protein YBL060W		PRO_0000120222
Regions
	Domain	61 – 266	206	SEC7
	Domain	412 – 551	140	PH
Amino acid modifications
	Modified residue	26	1	Phosphoserine Ref.3
	Modified residue	284	1	Phosphoserine Ref.4
	Modified residue	290	1	Phosphothreonine Ref.3 Ref.4
	Modified residue	293	1	Phosphoserine Ref.4
	Modified residue	297	1	Phosphoserine Ref.4
	Modified residue	303	1	Phosphoserine Ref.4
	Modified residue	306	1	Phosphoserine Ref.4

Sequences

	Sequence		Length	Mass (Da)
	P34225-1 [UniParc]. Last modified February 1, 1994. Version 1. Checksum: FBE491AABC5988E0	FASTA	687	78,768
	10 20 30 40 50 60 MCASLNEVKK NDTYGVSQKG YNDNFSESEG VLHGSKSMPT SMKNMLQSPT MVNMCDILQN 70 80 90 100 110 120 KEAANDEKPV IPTTDTATAG TGTEDISSTQ SEETDQNSHL IASEILEGTF KDVSYKEYAN 130 140 150 160 170 180 FLGNDNNNQV LTEFVKLLSP LPSSLLETLF NLSKSIYFIA EAQNIDRILE CLSIEWIACH 190 200 210 220 230 240 PNTHWKSGYK SCHIVLFSLL ILNSDLHNNF QVDHKKIKFS MVAFINNTLR ALREENEYEE 250 260 270 280 290 300 LKIYSREHLI IEELSEYYKT LNETPLPLCT ESRTSINISD NQSSLKRFST LGSREFSTSN 310 320 330 340 350 360 LRSVNSNSTT LYSRDGQVSV REMSAKSNKN FHNNHPMDAL YLKESFDDGL ITENGSSWFM 370 380 390 400 410 420 DDLILISKKS LPRKYSKRDK DQVAAPKMTS KRNKSFFGWL KPSKTTTLIE HTSRRTSLSY 430 440 450 460 470 480 LNKDSEWERV KIQVKEGRIF IFKIKPDVKD IIQSSETDSA TIDYFKDISS SYFAYSLLEA 490 500 510 520 530 540 EAHVVQDNII IGSGAMKSNV CNKNTKRKSG NFTVSFPENI NGPKLVLEFQ TRSVEEAHKF 550 560 570 580 590 600 MDCINFWAGR ISPVPLTQFE AVSNAEYGWS DKILTEHASL NLKNIVVSEW KPLLGLELLY 610 620 630 640 650 660 EDAKDVEMVE LKERLKELMN FTRQLGIWID KHNEIKDKLV EIWSFDDNYF EAVMNNWNSR 670 680 YLYMNNQYKK RLSYLKALQK AMGSVQF

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequencing and functional analysis of a 32,560 bp segment on the left arm of yeast chromosome II. Identification of 26 open reading frames, including the KIP1 and SEC17 genes." Scherens B., el Bakkoury M., Vierendeels F., Dubois E., Messenguy F. Yeast 9:1355-1371(1993) [PubMed: 8154187] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[2]	"Complete DNA sequence of yeast chromosome II." Feldmann H., Aigle M., Aljinovic G., Andre B., Baclet M.C., Barthe C., Baur A., Becam A.-M., Biteau N., Boles E., Brandt T., Brendel M., Brueckner M., Bussereau F., Christiansen C., Contreras R., Crouzet M., Cziepluch C. , Demolis N., Delaveau T., Doignon F., Domdey H., Duesterhus S., Dubois E., Dujon B., El Bakkoury M., Entian K.-D., Feuermann M., Fiers W., Fobo G.M., Fritz C., Gassenhuber J., Glansdorff N., Goffeau A., Grivell L.A., de Haan M., Hein C., Herbert C.J., Hollenberg C.P., Holmstroem K., Jacq C., Jacquet M., Jauniaux J.-C., Jonniaux J.-L., Kallesoee T., Kiesau P., Kirchrath L., Koetter P., Korol S., Liebl S., Logghe M., Lohan A.J.E., Louis E.J., Li Z.Y., Maat M.J., Mallet L., Mannhaupt G., Messenguy F., Miosga T., Molemans F., Mueller S., Nasr F., Obermaier B., Perea J., Pierard A., Piravandi E., Pohl F.M., Pohl T.M., Potier S., Proft M., Purnelle B., Ramezani Rad M., Rieger M., Rose M., Schaaff-Gerstenschlaeger I., Scherens B., Schwarzlose C., Skala J., Slonimski P.P., Smits P.H.M., Souciet J.-L., Steensma H.Y., Stucka R., Urrestarazu L.A., van der Aart Q.J.M., Van Dyck L., Vassarotti A., Vetter I., Vierendeels F., Vissers S., Wagner G., de Wergifosse P., Wolfe K.H., Zagulski M., Zimmermann F.K., Mewes H.-W., Kleine K. EMBO J. 13:5795-5809(1994) [PubMed: 7813418] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[3]	"Proteome-wide identification of in vivo targets of DNA damage checkpoint kinases." Smolka M.B., Albuquerque C.P., Chen S.H., Zhou H. Proc. Natl. Acad. Sci. U.S.A. 104:10364-10369(2007) [PubMed: 17563356] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-26 AND THR-290, MASS SPECTROMETRY.
[4]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed: 18407956] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-284; THR-290; SER-293; SER-297; SER-303 AND SER-306, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	Z23261 Genomic DNA. Translation: CAA80787.1. Z35821 Genomic DNA. Translation: CAA84880.1.
PIR	S39828.
RefSeq	NP_009493.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
IntAct	P34225. 1 interaction.
STRING	P34225.
Genome annotation databases
Ensembl	YBL060W; YBL060W; YBL060W; Saccharomyces cerevisiae. [Genome view]
GeneID	852219.
GenomeReviews	Gene locus YBL060W in contig Y13134_GR.
KEGG	sce:YBL060W.
NMPDR	fig|4932.3.peg.182.
Organism-specific databases
CYGD	YBL060w.
SGD	S000000156. YBL060W.
Phylogenomic databases
HOGENOM	P34225.
OMA	SKSIYFI.
Gene expression databases
ArrayExpress	P34225.
Genevestigator	P34225.
GermOnline	YBL060W. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR001849. Pleckstrin_homology. IPR000904. Sec7. [Graphical view]
Pfam	PF01369. Sec7. 1 hit. [Graphical view]
SMART	SM00233. PH. 1 hit. SM00222. Sec7. 1 hit. [Graphical view]
PROSITE	PS50003. PH_DOMAIN. False negative. PS50190. SEC7. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	970733.

Entry information

Entry name

YBG0_YEAST

Accession

Primary (citable) accession number: P34225

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1994
Last sequence update:	February 1, 1994
Last modified:	November 3, 2009
This is version 67 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)