P34225 (YEL1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Guanine-nucleotide exchange factor YEL1

Alternative name(s):
EFA6-like protein 1

Gene names

Name:	YEL1
Ordered Locus Names:	YBL060W
ORF Names:	YBL0507, YBL0517

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	687 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Guanine-nucleotide exchange factor for ARF3 required for localization of ARF3 to the bud neck and tip and involved in actin patch polarization. Ref.5 Ref.7 Ref.9
Subcellular location	Cytoplasm. Cell membrane; Peripheral membrane protein. Bud neck. Bud tip. Note: Localizes at the cell membrane only at the bud neck and bud tip and this localization is ARF3-dependent. Ref.4 Ref.5 Ref.7 Ref.9
Sequence similarities	Belongs to the YEL1 family. Contains 1 PH domain. Contains 1 SEC7 domain.

Ontologies

Keywords
Cellular component	Cell membrane Cytoplasm Membrane
Molecular function	Guanine-nucleotide releasing factor
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	actin cortical patch localization Inferred from mutant phenotype Ref.7. Source: SGD positive regulation of phosphatidylinositol biosynthetic process Inferred from mutant phenotype Ref.9. Source: SGD protein localization Inferred from mutant phenotype Ref.7. Source: SGD regulation of ARF protein signal transduction Inferred from electronic annotation. Source: InterPro
Cellular component	cellular bud neck Inferred from direct assay Ref.4Ref.5. Source: SGD cellular bud tip Inferred from direct assay Ref.5. Source: SGD cytoplasm Inferred from direct assay Ref.4. Source: SGD plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ARF guanyl-nucleotide exchange factor activity Inferred from direct assay Ref.5. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 687	687	Guanine-nucleotide exchange factor YEL1		PRO_0000120222
Regions
	Domain	61 – 266	206	SEC7
	Domain	412 – 551	140	PH
Amino acid modifications
	Modified residue	26	1	Phosphoserine Ref.6
	Modified residue	284	1	Phosphoserine Ref.8
	Modified residue	290	1	Phosphothreonine Ref.6 Ref.8
	Modified residue	293	1	Phosphoserine Ref.8
	Modified residue	297	1	Phosphoserine Ref.8
	Modified residue	303	1	Phosphoserine Ref.8
	Modified residue	306	1	Phosphoserine Ref.8

Sequences

	Sequence		Length	Mass (Da)
	P34225 [UniParc]. Last modified February 1, 1994. Version 1. Checksum: FBE491AABC5988E0	FASTA	687	78,768
	10 20 30 40 50 60 MCASLNEVKK NDTYGVSQKG YNDNFSESEG VLHGSKSMPT SMKNMLQSPT MVNMCDILQN 70 80 90 100 110 120 KEAANDEKPV IPTTDTATAG TGTEDISSTQ SEETDQNSHL IASEILEGTF KDVSYKEYAN 130 140 150 160 170 180 FLGNDNNNQV LTEFVKLLSP LPSSLLETLF NLSKSIYFIA EAQNIDRILE CLSIEWIACH 190 200 210 220 230 240 PNTHWKSGYK SCHIVLFSLL ILNSDLHNNF QVDHKKIKFS MVAFINNTLR ALREENEYEE 250 260 270 280 290 300 LKIYSREHLI IEELSEYYKT LNETPLPLCT ESRTSINISD NQSSLKRFST LGSREFSTSN 310 320 330 340 350 360 LRSVNSNSTT LYSRDGQVSV REMSAKSNKN FHNNHPMDAL YLKESFDDGL ITENGSSWFM 370 380 390 400 410 420 DDLILISKKS LPRKYSKRDK DQVAAPKMTS KRNKSFFGWL KPSKTTTLIE HTSRRTSLSY 430 440 450 460 470 480 LNKDSEWERV KIQVKEGRIF IFKIKPDVKD IIQSSETDSA TIDYFKDISS SYFAYSLLEA 490 500 510 520 530 540 EAHVVQDNII IGSGAMKSNV CNKNTKRKSG NFTVSFPENI NGPKLVLEFQ TRSVEEAHKF 550 560 570 580 590 600 MDCINFWAGR ISPVPLTQFE AVSNAEYGWS DKILTEHASL NLKNIVVSEW KPLLGLELLY 610 620 630 640 650 660 EDAKDVEMVE LKERLKELMN FTRQLGIWID KHNEIKDKLV EIWSFDDNYF EAVMNNWNSR 670 680 YLYMNNQYKK RLSYLKALQK AMGSVQF

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequencing and functional analysis of a 32,560 bp segment on the left arm of yeast chromosome II. Identification of 26 open reading frames, including the KIP1 and SEC17 genes." Scherens B., el Bakkoury M., Vierendeels F., Dubois E., Messenguy F. Yeast 9:1355-1371(1993) [PubMed: 8154187] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[2]	"Complete DNA sequence of yeast chromosome II." Feldmann H., Aigle M., Aljinovic G., Andre B., Baclet M.C., Barthe C., Baur A., Becam A.-M., Biteau N., Boles E., Brandt T., Brendel M., Brueckner M., Bussereau F., Christiansen C., Contreras R., Crouzet M., Cziepluch C. , Demolis N., Delaveau T., Doignon F., Domdey H., Duesterhus S., Dubois E., Dujon B., El Bakkoury M., Entian K.-D., Feuermann M., Fiers W., Fobo G.M., Fritz C., Gassenhuber J., Glansdorff N., Goffeau A., Grivell L.A., de Haan M., Hein C., Herbert C.J., Hollenberg C.P., Holmstroem K., Jacq C., Jacquet M., Jauniaux J.-C., Jonniaux J.-L., Kallesoee T., Kiesau P., Kirchrath L., Koetter P., Korol S., Liebl S., Logghe M., Lohan A.J.E., Louis E.J., Li Z.Y., Maat M.J., Mallet L., Mannhaupt G., Messenguy F., Miosga T., Molemans F., Mueller S., Nasr F., Obermaier B., Perea J., Pierard A., Piravandi E., Pohl F.M., Pohl T.M., Potier S., Proft M., Purnelle B., Ramezani Rad M., Rieger M., Rose M., Schaaff-Gerstenschlaeger I., Scherens B., Schwarzlose C., Skala J., Slonimski P.P., Smits P.H.M., Souciet J.-L., Steensma H.Y., Stucka R., Urrestarazu L.A., van der Aart Q.J.M., Van Dyck L., Vassarotti A., Vetter I., Vierendeels F., Vissers S., Wagner G., de Wergifosse P., Wolfe K.H., Zagulski M., Zimmermann F.K., Mewes H.-W., Kleine K. EMBO J. 13:5795-5809(1994) [PubMed: 7813418] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[3]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[4]	"Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed: 14562095] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[5]	"Identification of a guanine nucleotide exchange factor for Arf3, the yeast orthologue of mammalian Arf6." Gillingham A.K., Munro S. PLoS ONE 2:E842-E842(2007) [PubMed: 17786213] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION.
[6]	"Proteome-wide identification of in vivo targets of DNA damage checkpoint kinases." Smolka M.B., Albuquerque C.P., Chen S.H., Zhou H. Proc. Natl. Acad. Sci. U.S.A. 104:10364-10369(2007) [PubMed: 17563356] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-26 AND THR-290, MASS SPECTROMETRY.
[7]	"Afi1p functions as an Arf3p polarization-specific docking factor for development of polarity." Tsai P.-C., Lee S.-W., Liu Y.-W., Chu C.-W., Chen K.-Y., Ho J.-C., Lee F.-J. J. Biol. Chem. 283:16915-16927(2008) [PubMed: 18397879] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION.
[8]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed: 18407956] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-284; THR-290; SER-293; SER-297; SER-303 AND SER-306, MASS SPECTROMETRY.
[9]	"Yeast Arf3p modulates plasma membrane PtdIns(4,5)P2 levels to facilitate endocytosis." Smaczynska-de Rooij I.I., Costa R., Ayscough K.R. Traffic 9:559-573(2008) [PubMed: 18208507] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Z23261 Genomic DNA. Translation: CAA80787.1. Z35821 Genomic DNA. Translation: CAA84880.1. BK006936 Genomic DNA. Translation: DAA07059.1.
PIR	S39828.
RefSeq	NP_009493.1. NM_001178300.1.
3D structure databases
ProteinModelPortal	P34225.
SMR	P34225. Positions 75-269.
ModBase	Search...
Protein-protein interaction databases
IntAct	P34225. 1 interaction.
MINT	MINT-2491761.
STRING	P34225.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YBL060W; YBL060W; YBL060W.
GeneID	852219.
KEGG	sce:YBL060W.
NMPDR	fig|4932.3.peg.182.
Organism-specific databases
CYGD	YBL060w.
SGD	S000000156. YEL1.
Phylogenomic databases
eggNOG	fuNOG08985.
GeneTree	EFGT00050000002421.
OMA	SKSIYFI.
OrthoDB	EOG4VX5FD.
Gene expression databases
ArrayExpress	P34225.
Genevestigator	P34225.
GermOnline	YBL060W. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR001849. Pleckstrin_homology. IPR000904. Sec7. IPR023394. SEC7_alpha_orthog. [Graphical view]
Gene3D	G3DSA:1.10.1000.11. G3DSA:1.10.1000.11. 1 hit.
Pfam	PF01369. Sec7. 1 hit. [Graphical view]
SMART	SM00233. PH. 1 hit. SM00222. Sec7. 1 hit. [Graphical view]
SUPFAM	SSF48425. Sec7. 1 hit.
PROSITE	PS50003. PH_DOMAIN. False negative. PS50190. SEC7. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	970733.

Entry information

Entry name

YEL1_YEAST

Accession

Primary (citable) accession number: P34225
Secondary accession number(s): D6VPT9

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1994
Last sequence update:	February 1, 1994
Last modified:	December 14, 2011
This is version 84 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program