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P34223

- UBX1_YEAST

UniProt

P34223 - UBX1_YEAST

Protein

UBX domain-containing protein 1

Gene

SHP1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 126 (01 Oct 2014)
      Sequence version 1 (01 Feb 1994)
      Previous versions | rss
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    Functioni

    Involved in CDC48-dependent protein degradation through the ubiquitin/proteasome pathway. Direct or indirect positive regulator of GLC7 activity.1 Publication

    GO - Molecular functioni

    1. protein binding Source: IntAct
    2. protein phosphatase type 1 regulator activity Source: SGD

    GO - Biological processi

    1. ascospore formation Source: SGD
    2. autophagic vacuole assembly Source: SGD
    3. ER-associated ubiquitin-dependent protein catabolic process Source: SGD
    4. glycogen metabolic process Source: SGD
    5. piecemeal microautophagy of nucleus Source: SGD
    6. proteasome-mediated ubiquitin-dependent protein catabolic process Source: SGD
    7. regulation of catalytic activity Source: GOC
    8. sister chromatid biorientation Source: SGD

    Keywords - Biological processi

    Ubl conjugation pathway

    Enzyme and pathway databases

    BioCyciYEAST:G3O-28956-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    UBX domain-containing protein 1
    Alternative name(s):
    Suppressor of high-copy PP1 protein
    Gene namesi
    Name:SHP1
    Synonyms:UBX1
    Ordered Locus Names:YBL058W
    ORF Names:YBL0509, YBL0515
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome II

    Organism-specific databases

    CYGDiYBL058w.
    SGDiS000000154. SHP1.

    Subcellular locationi

    Nucleus 1 Publication. Cytoplasm 1 Publication

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell
    2. nucleus Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm, Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 423423UBX domain-containing protein 1PRO_0000210998Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei128 – 1281Phosphoserine1 Publication
    Modified residuei210 – 2101Phosphoserine1 Publication
    Modified residuei224 – 2241Phosphoserine1 Publication
    Modified residuei315 – 3151Phosphoserine4 Publications
    Modified residuei321 – 3211Phosphoserine1 Publication
    Modified residuei322 – 3221Phosphoserine2 Publications
    Modified residuei331 – 3311Phosphothreonine1 Publication

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiP34223.
    PaxDbiP34223.
    PeptideAtlasiP34223.

    Expressioni

    Gene expression databases

    GenevestigatoriP34223.

    Interactioni

    Subunit structurei

    Interacts with CDC48.1 Publication

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    CDC48P256949EBI-17093,EBI-4308
    DFM1Q127432EBI-17093,EBI-33192

    Protein-protein interaction databases

    BioGridi32641. 289 interactions.
    DIPiDIP-2789N.
    IntActiP34223. 9 interactions.
    MINTiMINT-656846.
    STRINGi4932.YBL058W.

    Structurei

    3D structure databases

    ProteinModelPortaliP34223.
    SMRiP34223. Positions 223-421.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini232 – 29766SEPPROSITE-ProRule annotationAdd
    BLAST
    Domaini344 – 42178UBXPROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Contains 1 SEP domain.PROSITE-ProRule annotation
    Contains 1 UBX domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiNOG252689.
    GeneTreeiENSGT00520000055567.
    HOGENOMiHOG000159961.
    KOiK14012.
    OMAiFILTHAF.
    OrthoDBiEOG7647C5.

    Family and domain databases

    InterProiIPR012989. SEP_domain.
    IPR029071. Ubiquitin-rel_dom.
    IPR001012. UBX_dom.
    [Graphical view]
    PfamiPF08059. SEP. 1 hit.
    PF00789. UBX. 1 hit.
    [Graphical view]
    SMARTiSM00553. SEP. 1 hit.
    SM00166. UBX. 1 hit.
    [Graphical view]
    SUPFAMiSSF102848. SSF102848. 1 hit.
    SSF54236. SSF54236. 1 hit.
    PROSITEiPS51399. SEP. 1 hit.
    PS50033. UBX. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P34223-1 [UniParc]FASTAAdd to Basket

    « Hide

    MAEIPDETIQ QFMALTNVSH NIAVQYLSEF GDLNEALNSY YASQTDDQKD    50
    RREEAHWNRQ QEKALKQEAF STNSSNKAIN TEHVGGLCPK PGSSQGSNEY 100
    LKRKGSTSPE PTKGSSRSGS GNNSRFMSFS DMVRGQADDD DEDQPRNTFA 150
    GGETSGLEVT DPSDPNSLLK DLLEKARRGG QMGAENGFRD DEDHEMGANR 200
    FTGRGFRLGS TIDAADEVVE DNTSQSQRRP EKVTREITFW KEGFQVADGP 250
    LYRYDDPANS FYLSELNQGR APLKLLDVQF GQEVEVNVYK KLDESYKAPT 300
    RKLGGFSGQG QRLGSPIPGE SSPAEVPKNE TPAAQEQPMP DNEPKQGDTS 350
    IQIRYANGKR EVLHCNSTDT VKFLYEHVTS NANTDPSRNF TLNYAFPIKP 400
    ISNDETTLKD ADLLNSVVVQ RWA 423
    Length:423
    Mass (Da):46,987
    Last modified:February 1, 1994 - v1
    Checksum:iAB46C88D7ED4F11C
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti223 – 2231T → A in AAS56043. (PubMed:17322287)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z23261 Genomic DNA. Translation: CAA80789.1.
    Z35819 Genomic DNA. Translation: CAA84878.1.
    AY557717 Genomic DNA. Translation: AAS56043.1.
    BK006936 Genomic DNA. Translation: DAA07062.1.
    PIRiS39830.
    RefSeqiNP_009495.1. NM_001178298.1.

    Genome annotation databases

    EnsemblFungiiYBL058W; YBL058W; YBL058W.
    GeneIDi852222.
    KEGGisce:YBL058W.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z23261 Genomic DNA. Translation: CAA80789.1 .
    Z35819 Genomic DNA. Translation: CAA84878.1 .
    AY557717 Genomic DNA. Translation: AAS56043.1 .
    BK006936 Genomic DNA. Translation: DAA07062.1 .
    PIRi S39830.
    RefSeqi NP_009495.1. NM_001178298.1.

    3D structure databases

    ProteinModelPortali P34223.
    SMRi P34223. Positions 223-421.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 32641. 289 interactions.
    DIPi DIP-2789N.
    IntActi P34223. 9 interactions.
    MINTi MINT-656846.
    STRINGi 4932.YBL058W.

    Proteomic databases

    MaxQBi P34223.
    PaxDbi P34223.
    PeptideAtlasi P34223.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YBL058W ; YBL058W ; YBL058W .
    GeneIDi 852222.
    KEGGi sce:YBL058W.

    Organism-specific databases

    CYGDi YBL058w.
    SGDi S000000154. SHP1.

    Phylogenomic databases

    eggNOGi NOG252689.
    GeneTreei ENSGT00520000055567.
    HOGENOMi HOG000159961.
    KOi K14012.
    OMAi FILTHAF.
    OrthoDBi EOG7647C5.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-28956-MONOMER.

    Miscellaneous databases

    NextBioi 970742.
    PROi P34223.

    Gene expression databases

    Genevestigatori P34223.

    Family and domain databases

    InterProi IPR012989. SEP_domain.
    IPR029071. Ubiquitin-rel_dom.
    IPR001012. UBX_dom.
    [Graphical view ]
    Pfami PF08059. SEP. 1 hit.
    PF00789. UBX. 1 hit.
    [Graphical view ]
    SMARTi SM00553. SEP. 1 hit.
    SM00166. UBX. 1 hit.
    [Graphical view ]
    SUPFAMi SSF102848. SSF102848. 1 hit.
    SSF54236. SSF54236. 1 hit.
    PROSITEi PS51399. SEP. 1 hit.
    PS50033. UBX. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Sequencing and functional analysis of a 32,560 bp segment on the left arm of yeast chromosome II. Identification of 26 open reading frames, including the KIP1 and SEC17 genes."
      Scherens B., el Bakkoury M., Vierendeels F., Dubois E., Messenguy F.
      Yeast 9:1355-1371(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    2. "Complete DNA sequence of yeast chromosome II."
      Feldmann H., Aigle M., Aljinovic G., Andre B., Baclet M.C., Barthe C., Baur A., Becam A.-M., Biteau N., Boles E., Brandt T., Brendel M., Brueckner M., Bussereau F., Christiansen C., Contreras R., Crouzet M., Cziepluch C.
      , Demolis N., Delaveau T., Doignon F., Domdey H., Duesterhus S., Dubois E., Dujon B., El Bakkoury M., Entian K.-D., Feuermann M., Fiers W., Fobo G.M., Fritz C., Gassenhuber J., Glansdorff N., Goffeau A., Grivell L.A., de Haan M., Hein C., Herbert C.J., Hollenberg C.P., Holmstroem K., Jacq C., Jacquet M., Jauniaux J.-C., Jonniaux J.-L., Kallesoee T., Kiesau P., Kirchrath L., Koetter P., Korol S., Liebl S., Logghe M., Lohan A.J.E., Louis E.J., Li Z.Y., Maat M.J., Mallet L., Mannhaupt G., Messenguy F., Miosga T., Molemans F., Mueller S., Nasr F., Obermaier B., Perea J., Pierard A., Piravandi E., Pohl F.M., Pohl T.M., Potier S., Proft M., Purnelle B., Ramezani Rad M., Rieger M., Rose M., Schaaff-Gerstenschlaeger I., Scherens B., Schwarzlose C., Skala J., Slonimski P.P., Smits P.H.M., Souciet J.-L., Steensma H.Y., Stucka R., Urrestarazu L.A., van der Aart Q.J.M., Van Dyck L., Vassarotti A., Vetter I., Vierendeels F., Vissers S., Wagner G., de Wergifosse P., Wolfe K.H., Zagulski M., Zimmermann F.K., Mewes H.-W., Kleine K.
      EMBO J. 13:5795-5809(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    3. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    4. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    5. "The Saccharomyces SHP1 gene, which encodes a regulator of phosphoprotein phosphatase 1 with differential effects on glycogen metabolism, meiotic differentiation, and mitotic cell cycle progression."
      Zhang S., Guha S., Volkert F.C.
      Mol. Cell. Biol. 15:2037-2050(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION.
    6. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
    7. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    8. "Shp1 and Ubx2 are adaptors of Cdc48 involved in ubiquitin-dependent protein degradation."
      Schuberth C., Richly H., Rumpf S., Buchberger A.
      EMBO Rep. 5:818-824(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, INTERACTION WITH CDC48.
    9. "Quantitative phosphoproteomics applied to the yeast pheromone signaling pathway."
      Gruhler A., Olsen J.V., Mohammed S., Mortensen P., Faergeman N.J., Mann M., Jensen O.N.
      Mol. Cell. Proteomics 4:310-327(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-315, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Strain: YAL6B.
    10. "Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
      Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
      J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-315 AND SER-322, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Strain: ADR376.
    11. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
      Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
      Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-315, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    12. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
      Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
      Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-128; SER-210; SER-224; SER-315; SER-321; SER-322 AND THR-331, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    13. "Sites of ubiquitin attachment in Saccharomyces cerevisiae."
      Starita L.M., Lo R.S., Eng J.K., von Haller P.D., Fields S.
      Proteomics 12:236-240(2012) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiUBX1_YEAST
    AccessioniPrimary (citable) accession number: P34223
    Secondary accession number(s): D6VPU2, E9P8S9
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1994
    Last sequence update: February 1, 1994
    Last modified: October 1, 2014
    This is version 126 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 3200 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    3. Yeast chromosome II
      Yeast (Saccharomyces cerevisiae) chromosome II: entries and gene names

    External Data

    Dasty 3