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P34223 (UBX1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 125. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
UBX domain-containing protein 1
Alternative name(s):
Suppressor of high-copy PP1 protein
Gene names
Name:SHP1
Synonyms:UBX1
Ordered Locus Names:YBL058W
ORF Names:YBL0509, YBL0515
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length423 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Involved in CDC48-dependent protein degradation through the ubiquitin/proteasome pathway. Direct or indirect positive regulator of GLC7 activity. Ref.8

Subunit structure

Interacts with CDC48. Ref.8

Subcellular location

Nucleus. Cytoplasm Ref.6.

Miscellaneous

Present with 3200 molecules/cell in log phase SD medium.

Sequence similarities

Contains 1 SEP domain.

Contains 1 UBX domain.

Binary interactions

With

Entry

#Exp.

IntAct

Notes

CDC48P256949EBI-17093,EBI-4308
DFM1Q127432EBI-17093,EBI-33192

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 423423UBX domain-containing protein 1
PRO_0000210998

Regions

Domain232 – 29766SEP
Domain344 – 42178UBX

Amino acid modifications

Modified residue1281Phosphoserine Ref.12
Modified residue2101Phosphoserine Ref.12
Modified residue2241Phosphoserine Ref.12
Modified residue3151Phosphoserine Ref.9 Ref.10 Ref.11 Ref.12
Modified residue3211Phosphoserine Ref.12
Modified residue3221Phosphoserine Ref.10 Ref.12
Modified residue3311Phosphothreonine Ref.12

Experimental info

Sequence conflict2231T → A in AAS56043. Ref.4

Sequences

Sequence LengthMass (Da)Tools
P34223 [UniParc].

Last modified February 1, 1994. Version 1.
Checksum: AB46C88D7ED4F11C

FASTA42346,987
        10         20         30         40         50         60 
MAEIPDETIQ QFMALTNVSH NIAVQYLSEF GDLNEALNSY YASQTDDQKD RREEAHWNRQ 

        70         80         90        100        110        120 
QEKALKQEAF STNSSNKAIN TEHVGGLCPK PGSSQGSNEY LKRKGSTSPE PTKGSSRSGS 

       130        140        150        160        170        180 
GNNSRFMSFS DMVRGQADDD DEDQPRNTFA GGETSGLEVT DPSDPNSLLK DLLEKARRGG 

       190        200        210        220        230        240 
QMGAENGFRD DEDHEMGANR FTGRGFRLGS TIDAADEVVE DNTSQSQRRP EKVTREITFW 

       250        260        270        280        290        300 
KEGFQVADGP LYRYDDPANS FYLSELNQGR APLKLLDVQF GQEVEVNVYK KLDESYKAPT 

       310        320        330        340        350        360 
RKLGGFSGQG QRLGSPIPGE SSPAEVPKNE TPAAQEQPMP DNEPKQGDTS IQIRYANGKR 

       370        380        390        400        410        420 
EVLHCNSTDT VKFLYEHVTS NANTDPSRNF TLNYAFPIKP ISNDETTLKD ADLLNSVVVQ 


RWA 

« Hide

References

« Hide 'large scale' references
[1]"Sequencing and functional analysis of a 32,560 bp segment on the left arm of yeast chromosome II. Identification of 26 open reading frames, including the KIP1 and SEC17 genes."
Scherens B., el Bakkoury M., Vierendeels F., Dubois E., Messenguy F.
Yeast 9:1355-1371(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[2]"Complete DNA sequence of yeast chromosome II."
Feldmann H., Aigle M., Aljinovic G., Andre B., Baclet M.C., Barthe C., Baur A., Becam A.-M., Biteau N., Boles E., Brandt T., Brendel M., Brueckner M., Bussereau F., Christiansen C., Contreras R., Crouzet M., Cziepluch C. expand/collapse author list , Demolis N., Delaveau T., Doignon F., Domdey H., Duesterhus S., Dubois E., Dujon B., El Bakkoury M., Entian K.-D., Feuermann M., Fiers W., Fobo G.M., Fritz C., Gassenhuber J., Glansdorff N., Goffeau A., Grivell L.A., de Haan M., Hein C., Herbert C.J., Hollenberg C.P., Holmstroem K., Jacq C., Jacquet M., Jauniaux J.-C., Jonniaux J.-L., Kallesoee T., Kiesau P., Kirchrath L., Koetter P., Korol S., Liebl S., Logghe M., Lohan A.J.E., Louis E.J., Li Z.Y., Maat M.J., Mallet L., Mannhaupt G., Messenguy F., Miosga T., Molemans F., Mueller S., Nasr F., Obermaier B., Perea J., Pierard A., Piravandi E., Pohl F.M., Pohl T.M., Potier S., Proft M., Purnelle B., Ramezani Rad M., Rieger M., Rose M., Schaaff-Gerstenschlaeger I., Scherens B., Schwarzlose C., Skala J., Slonimski P.P., Smits P.H.M., Souciet J.-L., Steensma H.Y., Stucka R., Urrestarazu L.A., van der Aart Q.J.M., Van Dyck L., Vassarotti A., Vetter I., Vierendeels F., Vissers S., Wagner G., de Wergifosse P., Wolfe K.H., Zagulski M., Zimmermann F.K., Mewes H.-W., Kleine K.
EMBO J. 13:5795-5809(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[3]"The reference genome sequence of Saccharomyces cerevisiae: Then and now."
Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R., Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S., Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.
G3 (Bethesda) 4:389-398(2014) [PubMed] [Europe PMC] [Abstract]
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[4]"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae."
Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. expand/collapse author list , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J.
Genome Res. 17:536-543(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[5]"The Saccharomyces SHP1 gene, which encodes a regulator of phosphoprotein phosphatase 1 with differential effects on glycogen metabolism, meiotic differentiation, and mitotic cell cycle progression."
Zhang S., Guha S., Volkert F.C.
Mol. Cell. Biol. 15:2037-2050(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION.
[6]"Global analysis of protein localization in budding yeast."
Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K.
Nature 425:686-691(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[7]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[8]"Shp1 and Ubx2 are adaptors of Cdc48 involved in ubiquitin-dependent protein degradation."
Schuberth C., Richly H., Rumpf S., Buchberger A.
EMBO Rep. 5:818-824(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, INTERACTION WITH CDC48.
[9]"Quantitative phosphoproteomics applied to the yeast pheromone signaling pathway."
Gruhler A., Olsen J.V., Mohammed S., Mortensen P., Faergeman N.J., Mann M., Jensen O.N.
Mol. Cell. Proteomics 4:310-327(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-315, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Strain: YAL6B.
[10]"Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-315 AND SER-322, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Strain: ADR376.
[11]"A multidimensional chromatography technology for in-depth phosphoproteome analysis."
Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-315, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[12]"Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-128; SER-210; SER-224; SER-315; SER-321; SER-322 AND THR-331, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[13]"Sites of ubiquitin attachment in Saccharomyces cerevisiae."
Starita L.M., Lo R.S., Eng J.K., von Haller P.D., Fields S.
Proteomics 12:236-240(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
Z23261 Genomic DNA. Translation: CAA80789.1.
Z35819 Genomic DNA. Translation: CAA84878.1.
AY557717 Genomic DNA. Translation: AAS56043.1.
BK006936 Genomic DNA. Translation: DAA07062.1.
PIRS39830.
RefSeqNP_009495.1. NM_001178298.1.

3D structure databases

ProteinModelPortalP34223.
SMRP34223. Positions 223-421.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid32641. 289 interactions.
DIPDIP-2789N.
IntActP34223. 9 interactions.
MINTMINT-656846.
STRING4932.YBL058W.

Proteomic databases

MaxQBP34223.
PaxDbP34223.
PeptideAtlasP34223.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYBL058W; YBL058W; YBL058W.
GeneID852222.
KEGGsce:YBL058W.

Organism-specific databases

CYGDYBL058w.
SGDS000000154. SHP1.

Phylogenomic databases

eggNOGNOG252689.
GeneTreeENSGT00520000055567.
HOGENOMHOG000159961.
KOK14012.
OMAFILTHAF.
OrthoDBEOG7647C5.

Enzyme and pathway databases

BioCycYEAST:G3O-28956-MONOMER.

Gene expression databases

GenevestigatorP34223.

Family and domain databases

InterProIPR012989. SEP_domain.
IPR029071. Ubiquitin-rel_dom.
IPR001012. UBX_dom.
[Graphical view]
PfamPF08059. SEP. 1 hit.
PF00789. UBX. 1 hit.
[Graphical view]
SMARTSM00553. SEP. 1 hit.
SM00166. UBX. 1 hit.
[Graphical view]
SUPFAMSSF102848. SSF102848. 1 hit.
SSF54236. SSF54236. 1 hit.
PROSITEPS51399. SEP. 1 hit.
PS50033. UBX. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio970742.
PROP34223.

Entry information

Entry nameUBX1_YEAST
AccessionPrimary (citable) accession number: P34223
Secondary accession number(s): D6VPU2, E9P8S9
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: February 1, 1994
Last modified: June 11, 2014
This is version 125 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome II

Yeast (Saccharomyces cerevisiae) chromosome II: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families