UBX domain-containing protein 1 - Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

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P34223 (UBX1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified May 29, 2013. Version 115. History...

Clusters with 100%, 90%, 50% identity |

Documents (3) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
UBX domain-containing protein 1

Alternative name(s):
Suppressor of high-copy PP1 protein

Gene names

Name:	SHP1
Synonyms:	UBX1
Ordered Locus Names:	YBL058W
ORF Names:	YBL0509, YBL0515

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces ›

Protein attributes

Sequence length	423 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Involved in CDC48-dependent protein degradation through the ubiquitin/proteasome pathway. Direct or indirect positive regulator of GLC7 activity. Ref.9
Subunit structure	Interacts with CDC48. Ref.9
Subcellular location	Nucleus. Cytoplasm Ref.7.
Miscellaneous	Present with 3200 molecules/cell in log phase SD medium.
Sequence similarities	Contains 1 SEP domain. Contains 1 UBX domain.

Ontologies

Keywords
Biological process	Ubl conjugation pathway
Cellular component	Cytoplasm Nucleus
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	ER-associated protein catabolic process Inferred from mutant phenotype PubMed 21148305. Source: SGD ascospore formation Inferred from mutant phenotype Ref.5. Source: SGD autophagic vacuole assembly Inferred from mutant phenotype PubMed 20855502. Source: SGD glycogen metabolic process Inferred from mutant phenotype Ref.5. Source: SGD piecemeal microautophagy of nucleus Inferred from mutant phenotype PubMed 20855502. Source: SGD sister chromatid biorientation Inferred from mutant phenotype PubMed 20483956. Source: SGD
Cellular_component	cytoplasm Inferred from direct assay Ref.7. Source: SGD nucleus Inferred from direct assay Ref.7. Source: SGD
Molecular_function	protein phosphatase type 1 regulator activity Inferred from mutant phenotype Ref.5. Source: SGD
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
CDC48	P25694	9	EBI-17093,EBI-4308

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 423

423

UBX domain-containing protein 1

PRO_0000210998

Regions

Domain

232 – 297

SEP

Domain

344 – 421

UBX

Amino acid modifications

Modified residue

Phosphoserine Ref.13

Modified residue

Phosphoserine Ref.13

Modified residue

Phosphoserine Ref.13

Modified residue

106

Phosphoserine Ref.6 Ref.13

Modified residue

108

Phosphoserine Ref.6 Ref.12 Ref.13

Modified residue

155

Phosphoserine Ref.13

Modified residue

210

Phosphoserine Ref.13

Modified residue

226

Phosphoserine Ref.13

Modified residue

315

Phosphoserine Ref.10 Ref.11 Ref.12 Ref.13

Modified residue

321

Phosphoserine Ref.12 Ref.13

Modified residue

322

Phosphoserine Ref.11 Ref.13

Experimental info

Sequence conflict

223

T → A in AAS56043. Ref.4

Sequences

Sequence

Length

Mass (Da)

Tools

P34223 [UniParc].

Last modified February 1, 1994. Version 1.
Checksum: AB46C88D7ED4F11C
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FASTA

423

46,987

        10         20         30         40         50         60 
MAEIPDETIQ QFMALTNVSH NIAVQYLSEF GDLNEALNSY YASQTDDQKD RREEAHWNRQ 

        70         80         90        100        110        120 
QEKALKQEAF STNSSNKAIN TEHVGGLCPK PGSSQGSNEY LKRKGSTSPE PTKGSSRSGS 

       130        140        150        160        170        180 
GNNSRFMSFS DMVRGQADDD DEDQPRNTFA GGETSGLEVT DPSDPNSLLK DLLEKARRGG 

       190        200        210        220        230        240 
QMGAENGFRD DEDHEMGANR FTGRGFRLGS TIDAADEVVE DNTSQSQRRP EKVTREITFW 

       250        260        270        280        290        300 
KEGFQVADGP LYRYDDPANS FYLSELNQGR APLKLLDVQF GQEVEVNVYK KLDESYKAPT 

       310        320        330        340        350        360 
RKLGGFSGQG QRLGSPIPGE SSPAEVPKNE TPAAQEQPMP DNEPKQGDTS IQIRYANGKR 

       370        380        390        400        410        420 
EVLHCNSTDT VKFLYEHVTS NANTDPSRNF TLNYAFPIKP ISNDETTLKD ADLLNSVVVQ 


RWA

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References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequencing and functional analysis of a 32,560 bp segment on the left arm of yeast chromosome II. Identification of 26 open reading frames, including the KIP1 and SEC17 genes." Scherens B., el Bakkoury M., Vierendeels F., Dubois E., Messenguy F. Yeast 9:1355-1371(1993) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[2]	"Complete DNA sequence of yeast chromosome II." Feldmann H., Aigle M., Aljinovic G., Andre B., Baclet M.C., Barthe C., Baur A., Becam A.-M., Biteau N., Boles E., Brandt T., Brendel M., Brueckner M., Bussereau F., Christiansen C., Contreras R., Crouzet M., Cziepluch C. Kleine K. EMBO J. 13:5795-5809(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[3]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[4]	"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae." Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. LaBaer J. Genome Res. 17:536-543(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[5]	"The Saccharomyces SHP1 gene, which encodes a regulator of phosphoprotein phosphatase 1 with differential effects on glycogen metabolism, meiotic differentiation, and mitotic cell cycle progression." Zhang S., Guha S., Volkert F.C. Mol. Cell. Biol. 15:2037-2050(1995) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION.
[6]	"Phosphoproteome analysis by mass spectrometry and its application to Saccharomyces cerevisiae." Ficarro S.B., McCleland M.L., Stukenberg P.T., Burke D.J., Ross M.M., Shabanowitz J., Hunt D.F., White F.M. Nat. Biotechnol. 20:301-305(2002) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-106 AND SER-108, MASS SPECTROMETRY. Strain: 2124.
[7]	"Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[8]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[9]	"Shp1 and Ubx2 are adaptors of Cdc48 involved in ubiquitin-dependent protein degradation." Schuberth C., Richly H., Rumpf S., Buchberger A. EMBO Rep. 5:818-824(2004) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, INTERACTION WITH CDC48.
[10]	"Quantitative phosphoproteomics applied to the yeast pheromone signaling pathway." Gruhler A., Olsen J.V., Mohammed S., Mortensen P., Faergeman N.J., Mann M., Jensen O.N. Mol. Cell. Proteomics 4:310-327(2005) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-315, MASS SPECTROMETRY. Strain: YAL6B.
[11]	"Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae." Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P. J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-315 AND SER-322, MASS SPECTROMETRY. Strain: ADR376.
[12]	"Proteome-wide identification of in vivo targets of DNA damage checkpoint kinases." Smolka M.B., Albuquerque C.P., Chen S.H., Zhou H. Proc. Natl. Acad. Sci. U.S.A. 104:10364-10369(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-108; SER-315 AND SER-321, MASS SPECTROMETRY.
[13]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-93; SER-94; SER-97; SER-106; SER-108; SER-155; SER-210; SER-226; SER-315; SER-321 AND SER-322, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Z23261 Genomic DNA. Translation: CAA80789.1. Z35819 Genomic DNA. Translation: CAA84878.1. AY557717 Genomic DNA. Translation: AAS56043.1. BK006936 Genomic DNA. Translation: DAA07062.1.
PIR	S39830.
RefSeq	NP_009495.1. NM_001178298.1.
3D structure databases
ProteinModelPortal	P34223.
SMR	P34223. Positions 3-46, 223-421.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-2789N.
IntAct	P34223. 10 interactions.
MINT	MINT-656846.
STRING	4932.YBL058W.
Proteomic databases
PaxDb	P34223.
PeptideAtlas	P34223.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YBL058W; YBL058W; YBL058W.
GeneID	852222.
KEGG	sce:YBL058W.
Organism-specific databases
CYGD	YBL058w.
SGD	S000000154. SHP1.
Phylogenomic databases
eggNOG	NOG252689.
GeneTree	ENSGT00520000055567.
HOGENOM	HOG000159961.
KO	K14012.
OMA	SFRDLMH.
OrthoDB	EOG41CB5C.
Enzyme and pathway databases
BioCyc	YEAST:G3O-28956-MONOMER.
Gene expression databases
Genevestigator	P34223.
GermOnline	YBL058W. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR012989. SEP_domain. IPR009060. UBA-like. IPR001012. UBX. [Graphical view]
Pfam	PF08059. SEP. 1 hit. PF00789. UBX. 1 hit. [Graphical view]
SMART	SM00553. SEP. 1 hit. SM00166. UBX. 1 hit. [Graphical view]
SUPFAM	SSF102848. SEP. 1 hit. SSF46934. UBA_like. 1 hit.
PROSITE	PS51399. SEP. 1 hit. PS50033. UBX. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	970742.

Entry information

Entry name

UBX1_YEAST

Accession

Primary (citable) accession number: P34223
Secondary accession number(s): D6VPU2, E9P8S9

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1994
Last sequence update:	February 1, 1994
Last modified:	May 29, 2013
This is version 115 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program

Relevant documents

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

Yeast chromosome II

Yeast (Saccharomyces cerevisiae) chromosome II: entries and gene names

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Binary interactions

With

Entry

#Exp.

IntAct

Notes

Sequence annotation (Features)

Molecule processing

Regions

Amino acid modifications

Experimental info

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents