Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

P34223

- UBX1_YEAST

UniProt

P34223 - UBX1_YEAST

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein

UBX domain-containing protein 1

Gene

SHP1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Involved in CDC48-dependent protein degradation through the ubiquitin/proteasome pathway. Direct or indirect positive regulator of GLC7 activity.1 Publication

GO - Molecular functioni

  1. protein phosphatase type 1 regulator activity Source: SGD

GO - Biological processi

  1. ascospore formation Source: SGD
  2. autophagic vacuole assembly Source: SGD
  3. ER-associated ubiquitin-dependent protein catabolic process Source: SGD
  4. glycogen metabolic process Source: SGD
  5. piecemeal microautophagy of nucleus Source: SGD
  6. proteasome-mediated ubiquitin-dependent protein catabolic process Source: SGD
  7. regulation of catalytic activity Source: GOC
  8. sister chromatid biorientation Source: SGD
Complete GO annotation...

Keywords - Biological processi

Ubl conjugation pathway

Enzyme and pathway databases

BioCyciYEAST:G3O-28956-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
UBX domain-containing protein 1
Alternative name(s):
Suppressor of high-copy PP1 protein
Gene namesi
Name:SHP1
Synonyms:UBX1
Ordered Locus Names:YBL058W
ORF Names:YBL0509, YBL0515
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome II

Organism-specific databases

CYGDiYBL058w.
SGDiS000000154. SHP1.

Subcellular locationi

Nucleus 1 Publication. Cytoplasm 1 Publication

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-KW
  2. nucleus Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 423423UBX domain-containing protein 1PRO_0000210998Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei128 – 1281Phosphoserine1 Publication
Modified residuei210 – 2101Phosphoserine1 Publication
Modified residuei224 – 2241Phosphoserine1 Publication
Modified residuei315 – 3151Phosphoserine4 Publications
Modified residuei321 – 3211Phosphoserine1 Publication
Modified residuei322 – 3221Phosphoserine2 Publications
Modified residuei331 – 3311Phosphothreonine1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP34223.
PaxDbiP34223.
PeptideAtlasiP34223.

Expressioni

Gene expression databases

GenevestigatoriP34223.

Interactioni

Subunit structurei

Interacts with CDC48.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
CDC48P256949EBI-17093,EBI-4308
DFM1Q127432EBI-17093,EBI-33192

Protein-protein interaction databases

BioGridi32641. 290 interactions.
DIPiDIP-2789N.
IntActiP34223. 9 interactions.
MINTiMINT-656846.
STRINGi4932.YBL058W.

Structurei

3D structure databases

ProteinModelPortaliP34223.
SMRiP34223. Positions 223-421.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini232 – 29766SEPPROSITE-ProRule annotationAdd
BLAST
Domaini344 – 42178UBXPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 SEP domain.PROSITE-ProRule annotation
Contains 1 UBX domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiNOG252689.
GeneTreeiENSGT00520000055567.
HOGENOMiHOG000159961.
KOiK14012.
OMAiFILTHAF.
OrthoDBiEOG7647C5.

Family and domain databases

InterProiIPR012989. SEP_domain.
IPR029071. Ubiquitin-rel_dom.
IPR001012. UBX_dom.
[Graphical view]
PfamiPF08059. SEP. 1 hit.
PF00789. UBX. 1 hit.
[Graphical view]
SMARTiSM00553. SEP. 1 hit.
SM00166. UBX. 1 hit.
[Graphical view]
SUPFAMiSSF102848. SSF102848. 1 hit.
SSF54236. SSF54236. 1 hit.
PROSITEiPS51399. SEP. 1 hit.
PS50033. UBX. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P34223-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MAEIPDETIQ QFMALTNVSH NIAVQYLSEF GDLNEALNSY YASQTDDQKD
60 70 80 90 100
RREEAHWNRQ QEKALKQEAF STNSSNKAIN TEHVGGLCPK PGSSQGSNEY
110 120 130 140 150
LKRKGSTSPE PTKGSSRSGS GNNSRFMSFS DMVRGQADDD DEDQPRNTFA
160 170 180 190 200
GGETSGLEVT DPSDPNSLLK DLLEKARRGG QMGAENGFRD DEDHEMGANR
210 220 230 240 250
FTGRGFRLGS TIDAADEVVE DNTSQSQRRP EKVTREITFW KEGFQVADGP
260 270 280 290 300
LYRYDDPANS FYLSELNQGR APLKLLDVQF GQEVEVNVYK KLDESYKAPT
310 320 330 340 350
RKLGGFSGQG QRLGSPIPGE SSPAEVPKNE TPAAQEQPMP DNEPKQGDTS
360 370 380 390 400
IQIRYANGKR EVLHCNSTDT VKFLYEHVTS NANTDPSRNF TLNYAFPIKP
410 420
ISNDETTLKD ADLLNSVVVQ RWA
Length:423
Mass (Da):46,987
Last modified:February 1, 1994 - v1
Checksum:iAB46C88D7ED4F11C
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti223 – 2231T → A in AAS56043. (PubMed:17322287)Curated

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Z23261 Genomic DNA. Translation: CAA80789.1.
Z35819 Genomic DNA. Translation: CAA84878.1.
AY557717 Genomic DNA. Translation: AAS56043.1.
BK006936 Genomic DNA. Translation: DAA07062.1.
PIRiS39830.
RefSeqiNP_009495.1. NM_001178298.1.

Genome annotation databases

EnsemblFungiiYBL058W; YBL058W; YBL058W.
GeneIDi852222.
KEGGisce:YBL058W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Z23261 Genomic DNA. Translation: CAA80789.1 .
Z35819 Genomic DNA. Translation: CAA84878.1 .
AY557717 Genomic DNA. Translation: AAS56043.1 .
BK006936 Genomic DNA. Translation: DAA07062.1 .
PIRi S39830.
RefSeqi NP_009495.1. NM_001178298.1.

3D structure databases

ProteinModelPortali P34223.
SMRi P34223. Positions 223-421.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 32641. 290 interactions.
DIPi DIP-2789N.
IntActi P34223. 9 interactions.
MINTi MINT-656846.
STRINGi 4932.YBL058W.

Proteomic databases

MaxQBi P34223.
PaxDbi P34223.
PeptideAtlasi P34223.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YBL058W ; YBL058W ; YBL058W .
GeneIDi 852222.
KEGGi sce:YBL058W.

Organism-specific databases

CYGDi YBL058w.
SGDi S000000154. SHP1.

Phylogenomic databases

eggNOGi NOG252689.
GeneTreei ENSGT00520000055567.
HOGENOMi HOG000159961.
KOi K14012.
OMAi FILTHAF.
OrthoDBi EOG7647C5.

Enzyme and pathway databases

BioCyci YEAST:G3O-28956-MONOMER.

Miscellaneous databases

NextBioi 970742.
PROi P34223.

Gene expression databases

Genevestigatori P34223.

Family and domain databases

InterProi IPR012989. SEP_domain.
IPR029071. Ubiquitin-rel_dom.
IPR001012. UBX_dom.
[Graphical view ]
Pfami PF08059. SEP. 1 hit.
PF00789. UBX. 1 hit.
[Graphical view ]
SMARTi SM00553. SEP. 1 hit.
SM00166. UBX. 1 hit.
[Graphical view ]
SUPFAMi SSF102848. SSF102848. 1 hit.
SSF54236. SSF54236. 1 hit.
PROSITEi PS51399. SEP. 1 hit.
PS50033. UBX. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Sequencing and functional analysis of a 32,560 bp segment on the left arm of yeast chromosome II. Identification of 26 open reading frames, including the KIP1 and SEC17 genes."
    Scherens B., el Bakkoury M., Vierendeels F., Dubois E., Messenguy F.
    Yeast 9:1355-1371(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  2. "Complete DNA sequence of yeast chromosome II."
    Feldmann H., Aigle M., Aljinovic G., Andre B., Baclet M.C., Barthe C., Baur A., Becam A.-M., Biteau N., Boles E., Brandt T., Brendel M., Brueckner M., Bussereau F., Christiansen C., Contreras R., Crouzet M., Cziepluch C.
    , Demolis N., Delaveau T., Doignon F., Domdey H., Duesterhus S., Dubois E., Dujon B., El Bakkoury M., Entian K.-D., Feuermann M., Fiers W., Fobo G.M., Fritz C., Gassenhuber J., Glansdorff N., Goffeau A., Grivell L.A., de Haan M., Hein C., Herbert C.J., Hollenberg C.P., Holmstroem K., Jacq C., Jacquet M., Jauniaux J.-C., Jonniaux J.-L., Kallesoee T., Kiesau P., Kirchrath L., Koetter P., Korol S., Liebl S., Logghe M., Lohan A.J.E., Louis E.J., Li Z.Y., Maat M.J., Mallet L., Mannhaupt G., Messenguy F., Miosga T., Molemans F., Mueller S., Nasr F., Obermaier B., Perea J., Pierard A., Piravandi E., Pohl F.M., Pohl T.M., Potier S., Proft M., Purnelle B., Ramezani Rad M., Rieger M., Rose M., Schaaff-Gerstenschlaeger I., Scherens B., Schwarzlose C., Skala J., Slonimski P.P., Smits P.H.M., Souciet J.-L., Steensma H.Y., Stucka R., Urrestarazu L.A., van der Aart Q.J.M., Van Dyck L., Vassarotti A., Vetter I., Vierendeels F., Vissers S., Wagner G., de Wergifosse P., Wolfe K.H., Zagulski M., Zimmermann F.K., Mewes H.-W., Kleine K.
    EMBO J. 13:5795-5809(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  4. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  5. "The Saccharomyces SHP1 gene, which encodes a regulator of phosphoprotein phosphatase 1 with differential effects on glycogen metabolism, meiotic differentiation, and mitotic cell cycle progression."
    Zhang S., Guha S., Volkert F.C.
    Mol. Cell. Biol. 15:2037-2050(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION.
  6. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
  7. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  8. "Shp1 and Ubx2 are adaptors of Cdc48 involved in ubiquitin-dependent protein degradation."
    Schuberth C., Richly H., Rumpf S., Buchberger A.
    EMBO Rep. 5:818-824(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH CDC48.
  9. "Quantitative phosphoproteomics applied to the yeast pheromone signaling pathway."
    Gruhler A., Olsen J.V., Mohammed S., Mortensen P., Faergeman N.J., Mann M., Jensen O.N.
    Mol. Cell. Proteomics 4:310-327(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-315, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Strain: YAL6B.
  10. "Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
    Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
    J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-315 AND SER-322, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Strain: ADR376.
  11. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
    Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
    Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-315, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  12. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
    Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
    Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-128; SER-210; SER-224; SER-315; SER-321; SER-322 AND THR-331, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  13. "Sites of ubiquitin attachment in Saccharomyces cerevisiae."
    Starita L.M., Lo R.S., Eng J.K., von Haller P.D., Fields S.
    Proteomics 12:236-240(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiUBX1_YEAST
AccessioniPrimary (citable) accession number: P34223
Secondary accession number(s): D6VPU2, E9P8S9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: February 1, 1994
Last modified: October 29, 2014
This is version 127 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 3200 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome II
    Yeast (Saccharomyces cerevisiae) chromosome II: entries and gene names

External Data

Dasty 3