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P34222 (PTH2_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 95. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Peptidyl-tRNA hydrolase 2

Short name=PTH 2
EC=3.1.1.29
Gene names
Name:PTH2
Ordered Locus Names:YBL057C
ORF Names:YBL0510, YBL0514
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length208 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

The natural substrate for this enzyme may be peptidyl-tRNAs which drop off the ribosome during protein synthesis By similarity. Ref.5 Ref.7

Catalytic activity

N-substituted aminoacyl-tRNA + H2O = N-substituted amino acid + tRNA.

Subcellular location

Cytoplasm.

Miscellaneous

Present with 2190 molecules/cell in log phase SD medium. Ref.6

Sequence similarities

Belongs to the PTH2 family.

Sequence caution

The sequence AAT92782.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.

The sequence CAA80790.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.

The sequence CAA84877.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.

Ontologies

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 208208Peptidyl-tRNA hydrolase 2
PRO_0000120284

Amino acid modifications

Cross-link152Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) Ref.8

Sequences

Sequence LengthMass (Da)Tools
P34222 [UniParc].

Last modified July 27, 2011. Version 2.
Checksum: A77F3A5BAC578943

FASTA20822,435
        10         20         30         40         50         60 
MEKMTVSSNY TIALWATFTA ISFAVGYQLG TSNASSTKKS SATLLRSKEM KEGKLHNDTD 

        70         80         90        100        110        120 
EEESESEDES DEDEDIESTS LNDIPGEVRM ALVIRQDLGM TKGKIAAQCC HAALSCFRHI 

       130        140        150        160        170        180 
ATNPARASYN PIMTQRWLNA GQAKITLKCP DKFTMDELYA KAISLGVNAA VIHDAGRTQI 

       190        200 
AAGSATVLGL GPAPKAVLDQ ITGDLKLY 

« Hide

References

« Hide 'large scale' references
[1]"Sequencing and functional analysis of a 32,560 bp segment on the left arm of yeast chromosome II. Identification of 26 open reading frames, including the KIP1 and SEC17 genes."
Scherens B., el Bakkoury M., Vierendeels F., Dubois E., Messenguy F.
Yeast 9:1355-1371(1993) [PubMed: 8154187] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[2]"Complete DNA sequence of yeast chromosome II."
Feldmann H., Aigle M., Aljinovic G., Andre B., Baclet M.C., Barthe C., Baur A., Becam A.-M., Biteau N., Boles E., Brandt T., Brendel M., Brueckner M., Bussereau F., Christiansen C., Contreras R., Crouzet M., Cziepluch C. expand/collapse author list , Demolis N., Delaveau T., Doignon F., Domdey H., Duesterhus S., Dubois E., Dujon B., El Bakkoury M., Entian K.-D., Feuermann M., Fiers W., Fobo G.M., Fritz C., Gassenhuber J., Glansdorff N., Goffeau A., Grivell L.A., de Haan M., Hein C., Herbert C.J., Hollenberg C.P., Holmstroem K., Jacq C., Jacquet M., Jauniaux J.-C., Jonniaux J.-L., Kallesoee T., Kiesau P., Kirchrath L., Koetter P., Korol S., Liebl S., Logghe M., Lohan A.J.E., Louis E.J., Li Z.Y., Maat M.J., Mallet L., Mannhaupt G., Messenguy F., Miosga T., Molemans F., Mueller S., Nasr F., Obermaier B., Perea J., Pierard A., Piravandi E., Pohl F.M., Pohl T.M., Potier S., Proft M., Purnelle B., Ramezani Rad M., Rieger M., Rose M., Schaaff-Gerstenschlaeger I., Scherens B., Schwarzlose C., Skala J., Slonimski P.P., Smits P.H.M., Souciet J.-L., Steensma H.Y., Stucka R., Urrestarazu L.A., van der Aart Q.J.M., Van Dyck L., Vassarotti A., Vetter I., Vierendeels F., Vissers S., Wagner G., de Wergifosse P., Wolfe K.H., Zagulski M., Zimmermann F.K., Mewes H.-W., Kleine K.
EMBO J. 13:5795-5809(1994) [PubMed: 7813418] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[3]Saccharomyces Genome Database
Submitted (MAR-2011) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[4]"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae."
Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. expand/collapse author list , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J.
Genome Res. 17:536-543(2007) [PubMed: 17322287] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[5]"Orthologs of a novel archaeal and of the bacterial peptidyl-tRNA hydrolase are nonessential in yeast."
Rosas-Sandoval G., Ambrogelly A., Rinehart J., Wei D., Cruz-Vera L.R., Graham D.E., Stetter K.O., Guarneros G., Soell D.
Proc. Natl. Acad. Sci. U.S.A. 99:16707-16712(2002) [PubMed: 12475929] [Abstract]
Cited for: FUNCTION.
[6]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed: 14562106] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[7]"Peptidyl-tRNA hydrolase from Sulfolobus solfataricus."
Fromant M., Ferri-Fioni M.-L., Plateau P., Blanquet S.
Nucleic Acids Res. 31:3227-3235(2003) [PubMed: 12799450] [Abstract]
Cited for: FUNCTION.
[8]"A subset of membrane-associated proteins is ubiquitinated in response to mutations in the endoplasmic reticulum degradation machinery."
Hitchcock A.L., Auld K., Gygi S.P., Silver P.A.
Proc. Natl. Acad. Sci. U.S.A. 100:12735-12740(2003) [PubMed: 14557538] [Abstract]
Cited for: UBIQUITINATION [LARGE SCALE ANALYSIS] AT LYS-152, MASS SPECTROMETRY.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
Z23261 Genomic DNA. Translation: CAA80790.1. Different initiation.
Z35818 Genomic DNA. Translation: CAA84877.1. Different initiation.
AY692763 Genomic DNA. Translation: AAT92782.1. Different initiation.
BK006936 Genomic DNA. Translation: DAA07063.2.
PIRS39831.
RefSeqNP_009496.2. NM_001178297.2.

3D structure databases

ProteinModelPortalP34222.
SMRP34222. Positions 86-208.
ModBaseSearch...

Protein-protein interaction databases

IntActP34222. 1 interaction.
MINTMINT-2732035.
STRINGP34222.

Proteomic databases

PeptideAtlasP34222.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID852223.
KEGGsce:YBL057C.
NMPDRfig|4932.3.peg.186.

Organism-specific databases

CYGDYBL057c.
SGDS000000153. PTH2.

Phylogenomic databases

eggNOGfuNOG09818.
GeneTreeEFGT00050000005903.
HOGENOMHBG526257.
OMACARVIQD.
OrthoDBEOG49GPRW.

Gene expression databases

ArrayExpressP34222.
GenevestigatorP34222.
GermOnlineYBL057C. Saccharomyces cerevisiae.

Family and domain databases

InterProIPR023476. Pep_tRNA_hydro_II_dom.
IPR002833. Pep_tRNA_hydro_PTH2.
[Graphical view]
Gene3DG3DSA:3.40.1490.10. G3DSA:3.40.1490.10. 1 hit.
KOK04794.
PfamPF01981. PTH2. 1 hit.
[Graphical view]
SUPFAMSSF102462. SSF102462. 1 hit.
TIGRFAMsTIGR00283. Arch_pth2. 1 hit.
ProtoNetSearch...

Other

NextBio970745.

Entry information

Entry namePTH2_YEAST
AccessionPrimary (citable) accession number: P34222
Secondary accession number(s): D6VPU3
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: July 27, 2011
Last modified: January 25, 2012
This is version 95 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

Yeast chromosome II

Yeast (Saccharomyces cerevisiae) chromosome II: entries and gene names

SIMILARITY comments

Index of protein domains and families