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P34221 (PP2C3_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 130. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Protein phosphatase 2C homolog 3

Short name=PP2C-3
EC=3.1.3.16
Gene names
Name:PTC3
Ordered Locus Names:YBL056W
ORF Names:YBL0511, YBL0513
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length468 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Responsible, together with PTC2, for the dephosphorylation of the cyclin-dependent protein kinase CDC28. Ref.6

Catalytic activity

[a protein]-serine/threonine phosphate + H2O = [a protein]-serine/threonine + phosphate.

Cofactor

Binds 2 magnesium or manganese ions per subunit By similarity.

Subcellular location

Cytoplasm. Nucleus Ref.7.

Miscellaneous

Present with 19700 molecules/cell in log phase SD medium.

Sequence similarities

Belongs to the PP2C family.

Binary interactions

With

Entry

#Exp.

IntAct

Notes

PAA1Q124476EBI-12805,EBI-33397

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 468468Protein phosphatase 2C homolog 3
PRO_0000057776

Sites

Metal binding621Manganese 1 By similarity
Metal binding621Manganese 2 By similarity
Metal binding631Manganese 1; via carbonyl oxygen By similarity
Metal binding2341Manganese 2 By similarity
Metal binding2841Manganese 2 By similarity

Amino acid modifications

Modified residue3241Phosphoserine Ref.9 Ref.10
Modified residue3321Phosphoserine Ref.9

Experimental info

Sequence conflict3691G → D in CAA80791. Ref.2
Sequence conflict3691G → D in CAA84876. Ref.3

Sequences

Sequence LengthMass (Da)Tools
P34221 [UniParc].

Last modified July 27, 2011. Version 4.
Checksum: 70AC2707C7FA2122

FASTA46851,332
        10         20         30         40         50         60 
MGQILSNPII DKEHHSGTDC LTAFGLCAMQ GWRMSMEDAH IVEPNLLAES DEEHLAFYGI 

        70         80         90        100        110        120 
FDGHGGSSVA EFCGSKMISI LKKQESFKSG MLEQCLIDTF LATDVELLKD EKLKDDHSGC 

       130        140        150        160        170        180 
TATVILVSQL KKLLICANSG DSRTVLSTGG NSKAMSFDHK PTLLSEKSRI VAADGFVEMD 

       190        200        210        220        230        240 
RVNGNLALSR AIGDFEFKSN TKLGPHEQVV TCVPDIICHN LNYDEDEFVI LACDGIWDCL 

       250        260        270        280        290        300 
TSQECVDLVH YGISQGNMTL SDISSRIVDV CCSPTTEGSG IGCDNMSISI VALLKENESE 

       310        320        330        340        350        360 
SQWFERMRSK NYNIQTSFVQ RRKSIFDFHD FSDDDNEVFA ITTKKLQDRL NRSKDNDDME 

       370        380        390        400        410        420 
IDDLDTELGS SATPSKLSGE DRTGPIDLFS LEALLEAGIQ IRQRPSSDSD GNTSYFHGAS 

       430        440        450        460 
LSDMLASLSN AAAGETEPND ADDNDDNDGE ENGKNENAKK GSKIEEIE 

« Hide

References

« Hide 'large scale' references
[1]Maeda T., Wurlger-Murphy S.M., Saito H.
Submitted (SEP-1996) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"Sequencing and functional analysis of a 32,560 bp segment on the left arm of yeast chromosome II. Identification of 26 open reading frames, including the KIP1 and SEC17 genes."
Scherens B., el Bakkoury M., Vierendeels F., Dubois E., Messenguy F.
Yeast 9:1355-1371(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[3]"Complete DNA sequence of yeast chromosome II."
Feldmann H., Aigle M., Aljinovic G., Andre B., Baclet M.C., Barthe C., Baur A., Becam A.-M., Biteau N., Boles E., Brandt T., Brendel M., Brueckner M., Bussereau F., Christiansen C., Contreras R., Crouzet M., Cziepluch C. expand/collapse author list , Demolis N., Delaveau T., Doignon F., Domdey H., Duesterhus S., Dubois E., Dujon B., El Bakkoury M., Entian K.-D., Feuermann M., Fiers W., Fobo G.M., Fritz C., Gassenhuber J., Glansdorff N., Goffeau A., Grivell L.A., de Haan M., Hein C., Herbert C.J., Hollenberg C.P., Holmstroem K., Jacq C., Jacquet M., Jauniaux J.-C., Jonniaux J.-L., Kallesoee T., Kiesau P., Kirchrath L., Koetter P., Korol S., Liebl S., Logghe M., Lohan A.J.E., Louis E.J., Li Z.Y., Maat M.J., Mallet L., Mannhaupt G., Messenguy F., Miosga T., Molemans F., Mueller S., Nasr F., Obermaier B., Perea J., Pierard A., Piravandi E., Pohl F.M., Pohl T.M., Potier S., Proft M., Purnelle B., Ramezani Rad M., Rieger M., Rose M., Schaaff-Gerstenschlaeger I., Scherens B., Schwarzlose C., Skala J., Slonimski P.P., Smits P.H.M., Souciet J.-L., Steensma H.Y., Stucka R., Urrestarazu L.A., van der Aart Q.J.M., Van Dyck L., Vassarotti A., Vetter I., Vierendeels F., Vissers S., Wagner G., de Wergifosse P., Wolfe K.H., Zagulski M., Zimmermann F.K., Mewes H.-W., Kleine K.
EMBO J. 13:5795-5809(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[4]"The reference genome sequence of Saccharomyces cerevisiae: Then and now."
Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R., Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S., Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.
G3 (Bethesda) 4:389-398(2014) [PubMed] [Europe PMC] [Abstract]
Cited for: GENOME REANNOTATION, SEQUENCE REVISION TO 369.
Strain: ATCC 204508 / S288c.
[5]"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae."
Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. expand/collapse author list , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J.
Genome Res. 17:536-543(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[6]"Dephosphorylation of cyclin-dependent kinases by type 2C protein phosphatases."
Cheng A., Ross K.E., Kaldis P., Solomon M.J.
Genes Dev. 13:2946-2957(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[7]"Global analysis of protein localization in budding yeast."
Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K.
Nature 425:686-691(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[8]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[9]"Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-324 AND SER-332, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Strain: ADR376.
[10]"Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-324, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U72346 Genomic DNA. Translation: AAB17351.1.
Z23261 Genomic DNA. Translation: CAA80791.1.
Z35817 Genomic DNA. Translation: CAA84876.1.
AY692754 Genomic DNA. Translation: AAT92773.1.
BK006936 Genomic DNA. Translation: DAA07064.2.
PIRS39832.
RefSeqNP_009497.2. NM_001178296.2.

3D structure databases

ProteinModelPortalP34221.
SMRP34221. Positions 1-275.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid32643. 46 interactions.
DIPDIP-3944N.
IntActP34221. 20 interactions.
MINTMINT-514472.
STRING4932.YBL056W.

Proteomic databases

MaxQBP34221.
PaxDbP34221.
PeptideAtlasP34221.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYBL056W; YBL056W; YBL056W.
GeneID852224.
KEGGsce:YBL056W.

Organism-specific databases

CYGDYBL056w.
SGDS000000152. PTC3.

Phylogenomic databases

eggNOGCOG0631.
GeneTreeENSGT00740000115384.
HOGENOMHOG000233896.
KOK14803.
OrthoDBEOG79930T.

Enzyme and pathway databases

BioCycYEAST:G3O-28954-MONOMER.

Gene expression databases

GenevestigatorP34221.

Family and domain databases

Gene3D3.60.40.10. 1 hit.
InterProIPR001932. PP2C-like_dom.
IPR000222. PP2C_Mn2_Asp60_BS.
IPR015655. Protein_Pase_2C.
[Graphical view]
PANTHERPTHR13832. PTHR13832. 1 hit.
PfamPF00481. PP2C. 1 hit.
[Graphical view]
SMARTSM00331. PP2C_SIG. 1 hit.
SM00332. PP2Cc. 1 hit.
[Graphical view]
SUPFAMSSF81606. SSF81606. 1 hit.
PROSITEPS01032. PP2C. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio970748.

Entry information

Entry namePP2C3_YEAST
AccessionPrimary (citable) accession number: P34221
Secondary accession number(s): D6VPU4, Q92330
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: July 27, 2011
Last modified: June 11, 2014
This is version 130 of the entry and version 4 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome II

Yeast (Saccharomyces cerevisiae) chromosome II: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families