Reviewed, UniProtKB/Swiss-Prot P34221 (PP2C3_YEAST)

Names and origin

Protein names

Recommended name:
    Protein phosphatase 2C homolog 3
      Short name=PP2C-3
    EC=3.1.3.16

Gene names

Name:	PTC3
Ordered Locus Names:	YBL056W
ORF Names:	YBL0511, YBL0513

Organism

Saccharomyces cerevisiae (Baker's yeast) [Complete proteome]

Taxonomic identifier

4932 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	468 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Responsible, together with PTC2, for the dephosphorylation of the cyclin-dependent protein kinase CDC28. Ref.5
Catalytic activity	A phosphoprotein + H2O = a protein + phosphate.
Cofactor	Binds 2 magnesium or manganese ions per subunit By similarity.
Miscellaneous	Present with 19700 molecules/cell in log phase SD medium. Ref.6
Sequence similarities	Belongs to the PP2C family.

Ontologies

Keywords
Ligand	Magnesium Manganese Metal-binding
Molecular function	Hydrolase Protein phosphatase
PTM	Phosphoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	inactivation of MAPK activity involved in osmosensory signaling pathway Inferred from genetic interaction. Source: SGD protein amino acid dephosphorylation Ref.5 Inferred from direct assay. Source: SGD regulation of cyclin-dependent protein kinase activity Ref.5 Inferred from mutant phenotype. Source: SGD
Cellular component	cytoplasm Inferred from direct assay. Source: SGD nucleus Inferred from direct assay. Source: SGD protein serine/threonine phosphatase complex Inferred from electronic annotation. Source: InterPro
Molecular function	magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW manganese ion binding Inferred from electronic annotation. Source: UniProtKB-KW protein binding Inferred from physical interaction. Source: IntAct protein serine/threonine phosphatase activity Ref.5 Inferred from direct assay. Source: SGD
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
	P42938	1	EBI-12805,EBI-23474
PAA1	Q12447	2	EBI-12805,EBI-33397
UBA1	P22515	1	EBI-12805,EBI-19703

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 468	468	Protein phosphatase 2C homolog 3		PRO_0000057776
Sites
	Metal binding	62	1	Manganese 1 By similarity
	Metal binding	62	1	Manganese 2 By similarity
	Metal binding	63	1	Manganese 1; via carbonyl oxygen By similarity
	Metal binding	234	1	Manganese 2 By similarity
	Metal binding	284	1	Manganese 2 By similarity
Amino acid modifications
	Modified residue	324	1	Phosphoserine Ref.7
	Modified residue	332	1	Phosphoserine Ref.7
Experimental info
	Sequence conflict	369	1	D → G Ref.1
	Sequence conflict	369	1	D → G Ref.4

Sequences

	Sequence		Length	Mass (Da)
	P34221-1 [UniParc]. Last modified December 20, 2005. Version 3. Checksum: DC5C18053828DC0D	FASTA	468	51,390
	10 20 30 40 50 60 MGQILSNPII DKEHHSGTDC LTAFGLCAMQ GWRMSMEDAH IVEPNLLAES DEEHLAFYGI 70 80 90 100 110 120 FDGHGGSSVA EFCGSKMISI LKKQESFKSG MLEQCLIDTF LATDVELLKD EKLKDDHSGC 130 140 150 160 170 180 TATVILVSQL KKLLICANSG DSRTVLSTGG NSKAMSFDHK PTLLSEKSRI VAADGFVEMD 190 200 210 220 230 240 RVNGNLALSR AIGDFEFKSN TKLGPHEQVV TCVPDIICHN LNYDEDEFVI LACDGIWDCL 250 260 270 280 290 300 TSQECVDLVH YGISQGNMTL SDISSRIVDV CCSPTTEGSG IGCDNMSISI VALLKENESE 310 320 330 340 350 360 SQWFERMRSK NYNIQTSFVQ RRKSIFDFHD FSDDDNEVFA ITTKKLQDRL NRSKDNDDME 370 380 390 400 410 420 IDDLDTELDS SATPSKLSGE DRTGPIDLFS LEALLEAGIQ IRQRPSSDSD GNTSYFHGAS 430 440 450 460 LSDMLASLSN AAAGETEPND ADDNDDNDGE ENGKNENAKK GSKIEEIE

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	Maeda T., Wurlger-Murphy S.M., Saito H. Submitted (SEP-1996) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Sequencing and functional analysis of a 32,560 bp segment on the left arm of yeast chromosome II. Identification of 26 open reading frames, including the KIP1 and SEC17 genes." Scherens B., el Bakkoury M., Vierendeels F., Dubois E., Messenguy F. Yeast 9:1355-1371(1993) [PubMed: 8154187] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[3]	"Complete DNA sequence of yeast chromosome II." Feldmann H., Aigle M., Aljinovic G., Andre B., Baclet M.C., Barthe C., Baur A., Becam A.-M., Biteau N., Boles E., Brandt T., Brendel M., Brueckner M., Bussereau F., Christiansen C., Contreras R., Crouzet M., Cziepluch C. , Demolis N., Delaveau T., Doignon F., Domdey H., Duesterhus S., Dubois E., Dujon B., El Bakkoury M., Entian K.-D., Feuermann M., Fiers W., Fobo G.M., Fritz C., Gassenhuber J., Glansdorff N., Goffeau A., Grivell L.A., de Haan M., Hein C., Herbert C.J., Hollenberg C.P., Holmstroem K., Jacq C., Jacquet M., Jauniaux J.-C., Jonniaux J.-L., Kallesoee T., Kiesau P., Kirchrath L., Koetter P., Korol S., Liebl S., Logghe M., Lohan A.J.E., Louis E.J., Li Z.Y., Maat M.J., Mallet L., Mannhaupt G., Messenguy F., Miosga T., Molemans F., Mueller S., Nasr F., Obermaier B., Perea J., Pierard A., Piravandi E., Pohl F.M., Pohl T.M., Potier S., Proft M., Purnelle B., Ramezani Rad M., Rieger M., Rose M., Schaaff-Gerstenschlaeger I., Scherens B., Schwarzlose C., Skala J., Slonimski P.P., Smits P.H.M., Souciet J.-L., Steensma H.Y., Stucka R., Urrestarazu L.A., van der Aart Q.J.M., Van Dyck L., Vassarotti A., Vetter I., Vierendeels F., Vissers S., Wagner G., de Wergifosse P., Wolfe K.H., Zagulski M., Zimmermann F.K., Mewes H.-W., Kleine K. EMBO J. 13:5795-5809(1994) [PubMed: 7813418] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[4]	"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae." Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J. Genome Res. 17:536-543(2007) [PubMed: 17322287] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[5]	"Dephosphorylation of cyclin-dependent kinases by type 2C protein phosphatases." Cheng A., Ross K.E., Kaldis P., Solomon M.J. Genes Dev. 13:2946-2957(1999) [PubMed: 10580002] [Abstract] Cited for: FUNCTION.
[6]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[7]	"Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae." Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P. J. Proteome Res. 6:1190-1197(2007) [PubMed: 17330950] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-324 AND SER-332, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	U72346 Genomic DNA. Translation: AAB17351.1. Z23261 Genomic DNA. Translation: CAA80791.1. Z35817 Genomic DNA. Translation: CAA84876.1. AY692754 Genomic DNA. Translation: AAT92773.1.
PIR	S39832.
RefSeq	NP_009497.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
DIP	DIP:3944N.
IntAct	P34221. 28 interactions.
STRING	P34221.
Proteomic databases
PeptideAtlas	P34221.
Genome annotation databases
Ensembl	YBL056W; YBL056W; YBL056W; Saccharomyces cerevisiae. [Genome view]
GeneID	852224.
KEGG	sce:YBL056W.
NMPDR	fig|4932.3.peg.187.
Organism-specific databases
CYGD	YBL056w.
SGD	S000000152. PTC3.
Phylogenomic databases
HOGENOM	P34221.
OMA	EDAHIVE
OrthoDB	EOG966X32
Enzyme and pathway databases
BRENDA	3.1.3.16. 250.
Gene expression databases
ArrayExpress	P34221.
Genevestigator	P34221.
GermOnline	YBL056W. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR015655. PP2C. IPR001932. PP2C-related. IPR000222. PP2C_Mn2_Asp60_BS. IPR014045. PP2C_N. [Graphical view]
Gene3D	G3DSA:3.60.40.10. PP2C-related. 1 hit.
PANTHER	PTHR13832. PP2C. 1 hit.
Pfam	PF00481. PP2C. 1 hit. [Graphical view]
SMART	SM00331. PP2C_SIG. 1 hit. SM00332. PP2Cc. 1 hit. [Graphical view]
PROSITE	PS01032. PP2C. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	970748.

Entry information

Entry name

PP2C3_YEAST

Accession

Primary (citable) accession number: P34221
Secondary accession number(s): Q92330

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1994
Last sequence update:	December 20, 2005
Last modified:	November 24, 2009
This is version 87 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)