Reviewed,
UniProtKB/Swiss-Prot P34221 (PP2C3_YEAST)
Last modified
November 24, 2009.
Version 87.
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Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Binary interactions · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents
Names and origin
| Protein names | Recommended name: Protein phosphatase 2C homolog 3 Short name=PP2C-3 EC=3.1.3.16 | ||||||
| Gene names |
| ||||||
| Organism | Saccharomyces cerevisiae (Baker's yeast) [Complete proteome] | ||||||
| Taxonomic identifier | 4932 [NCBI] | ||||||
| Taxonomic lineage | Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces |
Protein attributes
| Sequence length | 468 AA. |
| Sequence status | Complete. |
| Sequence processing | The displayed sequence is not processed. |
| Protein existence | Evidence at protein level. |
General annotation (Comments)
| Function | Responsible, together with PTC2, for the dephosphorylation of the cyclin-dependent protein kinase CDC28. Ref.5 |
| Catalytic activity | A phosphoprotein + H2O = a protein + phosphate. |
| Cofactor | Binds 2 magnesium or manganese ions per subunit By similarity. |
| Miscellaneous | Present with 19700 molecules/cell in log phase SD medium. Ref.6 |
| Sequence similarities | Belongs to the PP2C family. |
Ontologies
Binary interactions
With | Entry | #Exp. | IntAct | Notes |
|---|---|---|---|---|
| P42938 | 1 | EBI-12805,EBI-23474 | ||
| PAA1 | Q12447 | 2 | EBI-12805,EBI-33397 | |
| UBA1 | P22515 | 1 | EBI-12805,EBI-19703 |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Chain | 1 – 468 | 468 | Protein phosphatase 2C homolog 3 | PRO_0000057776 | |||||
Sites | |||||||||
| Metal binding | 62 | 1 | Manganese 1 By similarity | ||||||
| Metal binding | 62 | 1 | Manganese 2 By similarity | ||||||
| Metal binding | 63 | 1 | Manganese 1; via carbonyl oxygen By similarity | ||||||
| Metal binding | 234 | 1 | Manganese 2 By similarity | ||||||
| Metal binding | 284 | 1 | Manganese 2 By similarity | ||||||
Amino acid modifications | |||||||||
| Modified residue | 324 | 1 | Phosphoserine Ref.7 | ||||||
| Modified residue | 332 | 1 | Phosphoserine Ref.7 | ||||||
Experimental info | |||||||||
| Sequence conflict | 369 | 1 | D → G Ref.1 | ||||||
| Sequence conflict | 369 | 1 | D → G Ref.4 | ||||||
Sequences
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References
| « Hide 'large scale' references | |
| [1] | Maeda T., Wurlger-Murphy S.M., Saito H. Submitted (SEP-1996) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. |
| [2] | "Sequencing and functional analysis of a 32,560 bp segment on the left arm of yeast chromosome II. Identification of 26 open reading frames, including the KIP1 and SEC17 genes." Scherens B., el Bakkoury M., Vierendeels F., Dubois E., Messenguy F. Yeast 9:1355-1371(1993) [PubMed: 8154187] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c. |
| [3] | "Complete DNA sequence of yeast chromosome II." Feldmann H., Aigle M., Aljinovic G., Andre B., Baclet M.C., Barthe C., Baur A., Becam A.-M., Biteau N., Boles E., Brandt T., Brendel M., Brueckner M., Bussereau F., Christiansen C., Contreras R., Crouzet M., Cziepluch C. Kleine K.EMBO J. 13:5795-5809(1994) [PubMed: 7813418] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204508 / S288c. |
| [4] | "Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae." Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. LaBaer J.Genome Res. 17:536-543(2007) [PubMed: 17322287] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c. |
| [5] | "Dephosphorylation of cyclin-dependent kinases by type 2C protein phosphatases." Cheng A., Ross K.E., Kaldis P., Solomon M.J. Genes Dev. 13:2946-2957(1999) [PubMed: 10580002] [Abstract] Cited for: FUNCTION. |
| [6] | "Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS]. |
| [7] | "Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae." Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P. J. Proteome Res. 6:1190-1197(2007) [PubMed: 17330950] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-324 AND SER-332, MASS SPECTROMETRY. |
| + | Additional computationally mapped references. |
Cross-references
Sequence databases | |
|---|---|
| U72346 Genomic DNA. Translation: AAB17351.1. Z23261 Genomic DNA. Translation: CAA80791.1. Z35817 Genomic DNA. Translation: CAA84876.1. AY692754 Genomic DNA. Translation: AAT92773.1. | |
| PIR | S39832. |
| RefSeq | NP_009497.1. |
3D structure databases | |
| ModBase | Search... |
Protein-protein interaction databases | |
| DIP | DIP:3944N. |
| IntAct | P34221. 28 interactions. |
| STRING | P34221. |
Proteomic databases | |
| PeptideAtlas | P34221. |
Genome annotation databases | |
| Ensembl | YBL056W; YBL056W; YBL056W; Saccharomyces cerevisiae. [Genome view] |
| GeneID | 852224. |
| KEGG | sce:YBL056W. |
| NMPDR | fig|4932.3.peg.187. |
Organism-specific databases | |
| CYGD | YBL056w. |
| SGD | S000000152. PTC3. |
Phylogenomic databases | |
| HOGENOM | P34221. |
| OMA | EDAHIVE |
| OrthoDB | EOG966X32 |
Enzyme and pathway databases | |
| BRENDA | 3.1.3.16. 250. |
Gene expression databases | |
| ArrayExpress | P34221. |
| Genevestigator | P34221. |
| GermOnline | YBL056W. Saccharomyces cerevisiae. |
Family and domain databases | |
| InterPro | IPR015655. PP2C. IPR001932. PP2C-related. IPR000222. PP2C_Mn2_Asp60_BS. IPR014045. PP2C_N. [Graphical view] |
| Gene3D | G3DSA:3.60.40.10. PP2C-related. 1 hit. |
| PANTHER | PTHR13832. PP2C. 1 hit. |
| Pfam | PF00481. PP2C. 1 hit. [Graphical view] |
| SMART | SM00331. PP2C_SIG. 1 hit. SM00332. PP2Cc. 1 hit. [Graphical view] |
| PROSITE | PS01032. PP2C. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Other Resources | |
| NextBio | 970748. |
Entry information
| Entry name | PP2C3_YEAST | ||||||||
| Accession | Primary (citable) accession number: P34221 Secondary accession number(s): Q92330 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | FPAP (Fungal Proteome Annotation Project) | ||||||||
Relevant documents
| SIMILARITY comments Index of protein domains and families |
| Yeast Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD |
| Yeast chromosome II Yeast (Saccharomyces cerevisiae) chromosome II: entries and gene names |

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