P34221 (PP2C3_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Protein phosphatase 2C homolog 3
Short name=PP2C-3
EC=3.1.3.16

Gene names

Name:	PTC3
Ordered Locus Names:	YBL056W
ORF Names:	YBL0511, YBL0513

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › Saccharomyces cerevisiae

Protein attributes

Sequence length	468 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Responsible, together with PTC2, for the dephosphorylation of the cyclin-dependent protein kinase CDC28. Ref.6
Catalytic activity	A phosphoprotein + H2O = a protein + phosphate.
Cofactor	Binds 2 magnesium or manganese ions per subunit By similarity.
Subcellular location	Cytoplasm. Nucleus Ref.7.
Miscellaneous	Present with 19700 molecules/cell in log phase SD medium.
Sequence similarities	Belongs to the PP2C family.

Ontologies

Keywords
Cellular component	Cytoplasm Nucleus
Ligand	Magnesium Manganese Metal-binding
Molecular function	Hydrolase Protein phosphatase
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	inactivation of MAPK activity involved in osmosensory signaling pathway Inferred from genetic interaction PubMed 11113180PubMed 12477803. Source: SGD protein dephosphorylation Inferred from direct assay Ref.6. Source: SGD regulation of cyclin-dependent protein serine/threonine kinase activity Inferred from mutant phenotype Ref.6. Source: SGD
Cellular_component	cytoplasm Inferred from direct assay Ref.7. Source: SGD nucleus Inferred from direct assay Ref.7. Source: SGD
Molecular_function	metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW protein serine/threonine phosphatase activity Inferred from direct assay Ref.6. Source: SGD
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
PAA1	Q12447	7	EBI-12805,EBI-33397

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 468	468	Protein phosphatase 2C homolog 3		PRO_0000057776
Sites
	Metal binding	62	1	Manganese 1 By similarity
	Metal binding	62	1	Manganese 2 By similarity
	Metal binding	63	1	Manganese 1; via carbonyl oxygen By similarity
	Metal binding	234	1	Manganese 2 By similarity
	Metal binding	284	1	Manganese 2 By similarity
Amino acid modifications
	Modified residue	324	1	Phosphoserine Ref.9
	Modified residue	332	1	Phosphoserine Ref.9
Experimental info
	Sequence conflict	369	1	G → D in CAA80791. Ref.2
	Sequence conflict	369	1	G → D in CAA84876. Ref.3

Sequences

	Sequence		Length	Mass (Da)
	P34221 [UniParc]. Last modified July 27, 2011. Version 4. Checksum: 70AC2707C7FA2122	FASTA	468	51,332
	10 20 30 40 50 60 MGQILSNPII DKEHHSGTDC LTAFGLCAMQ GWRMSMEDAH IVEPNLLAES DEEHLAFYGI 70 80 90 100 110 120 FDGHGGSSVA EFCGSKMISI LKKQESFKSG MLEQCLIDTF LATDVELLKD EKLKDDHSGC 130 140 150 160 170 180 TATVILVSQL KKLLICANSG DSRTVLSTGG NSKAMSFDHK PTLLSEKSRI VAADGFVEMD 190 200 210 220 230 240 RVNGNLALSR AIGDFEFKSN TKLGPHEQVV TCVPDIICHN LNYDEDEFVI LACDGIWDCL 250 260 270 280 290 300 TSQECVDLVH YGISQGNMTL SDISSRIVDV CCSPTTEGSG IGCDNMSISI VALLKENESE 310 320 330 340 350 360 SQWFERMRSK NYNIQTSFVQ RRKSIFDFHD FSDDDNEVFA ITTKKLQDRL NRSKDNDDME 370 380 390 400 410 420 IDDLDTELGS SATPSKLSGE DRTGPIDLFS LEALLEAGIQ IRQRPSSDSD GNTSYFHGAS 430 440 450 460 LSDMLASLSN AAAGETEPND ADDNDDNDGE ENGKNENAKK GSKIEEIE

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	Maeda T., Wurlger-Murphy S.M., Saito H. Submitted (SEP-1996) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Sequencing and functional analysis of a 32,560 bp segment on the left arm of yeast chromosome II. Identification of 26 open reading frames, including the KIP1 and SEC17 genes." Scherens B., el Bakkoury M., Vierendeels F., Dubois E., Messenguy F. Yeast 9:1355-1371(1993) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[3]	"Complete DNA sequence of yeast chromosome II." Feldmann H., Aigle M., Aljinovic G., Andre B., Baclet M.C., Barthe C., Baur A., Becam A.-M., Biteau N., Boles E., Brandt T., Brendel M., Brueckner M., Bussereau F., Christiansen C., Contreras R., Crouzet M., Cziepluch C. , Demolis N., Delaveau T., Doignon F., Domdey H., Duesterhus S., Dubois E., Dujon B., El Bakkoury M., Entian K.-D., Feuermann M., Fiers W., Fobo G.M., Fritz C., Gassenhuber J., Glansdorff N., Goffeau A., Grivell L.A., de Haan M., Hein C., Herbert C.J., Hollenberg C.P., Holmstroem K., Jacq C., Jacquet M., Jauniaux J.-C., Jonniaux J.-L., Kallesoee T., Kiesau P., Kirchrath L., Koetter P., Korol S., Liebl S., Logghe M., Lohan A.J.E., Louis E.J., Li Z.Y., Maat M.J., Mallet L., Mannhaupt G., Messenguy F., Miosga T., Molemans F., Mueller S., Nasr F., Obermaier B., Perea J., Pierard A., Piravandi E., Pohl F.M., Pohl T.M., Potier S., Proft M., Purnelle B., Ramezani Rad M., Rieger M., Rose M., Schaaff-Gerstenschlaeger I., Scherens B., Schwarzlose C., Skala J., Slonimski P.P., Smits P.H.M., Souciet J.-L., Steensma H.Y., Stucka R., Urrestarazu L.A., van der Aart Q.J.M., Van Dyck L., Vassarotti A., Vetter I., Vierendeels F., Vissers S., Wagner G., de Wergifosse P., Wolfe K.H., Zagulski M., Zimmermann F.K., Mewes H.-W., Kleine K. EMBO J. 13:5795-5809(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[4]	Saccharomyces Genome Database Submitted (MAR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION, SEQUENCE REVISION TO 369. Strain: ATCC 204508 / S288c.
[5]	"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae." Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J. Genome Res. 17:536-543(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[6]	"Dephosphorylation of cyclin-dependent kinases by type 2C protein phosphatases." Cheng A., Ross K.E., Kaldis P., Solomon M.J. Genes Dev. 13:2946-2957(1999) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
[7]	"Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[8]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[9]	"Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae." Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P. J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-324 AND SER-332, MASS SPECTROMETRY. Strain: ADR376.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U72346 Genomic DNA. Translation: AAB17351.1. Z23261 Genomic DNA. Translation: CAA80791.1. Z35817 Genomic DNA. Translation: CAA84876.1. AY692754 Genomic DNA. Translation: AAT92773.1. BK006936 Genomic DNA. Translation: DAA07064.2.
PIR	S39832.
RefSeq	NP_009497.2. NM_001178296.2.
3D structure databases
ProteinModelPortal	P34221.
SMR	P34221. Positions 1-275.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-3944N.
IntAct	P34221. 26 interactions.
MINT	MINT-514472.
STRING	4932.YBL056W.
Proteomic databases
PaxDb	P34221.
PeptideAtlas	P34221.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YBL056W; YBL056W; YBL056W.
GeneID	852224.
KEGG	sce:YBL056W.
Organism-specific databases
CYGD	YBL056w.
SGD	S000000152. PTC3.
Phylogenomic databases
eggNOG	COG0631.
GeneTree	ENSGT00650000093052.
HOGENOM	HOG000233896.
KO	K14803.
OMA	HAGRING.
OrthoDB	EOG4RFQ28.
Enzyme and pathway databases
BioCyc	YEAST:G3O-28954-MONOMER.
Gene expression databases
Genevestigator	P34221.
GermOnline	YBL056W. Saccharomyces cerevisiae.
Family and domain databases
Gene3D	3.60.40.10. 1 hit.
InterPro	IPR001932. PP2C-like. IPR000222. PP2C_Mn2_Asp60_BS. IPR015655. Protein_Pase_2C. [Graphical view]
PANTHER	PTHR13832. PTHR13832. 1 hit.
Pfam	PF00481. PP2C. 1 hit. [Graphical view]
SMART	SM00331. PP2C_SIG. 1 hit. SM00332. PP2Cc. 1 hit. [Graphical view]
SUPFAM	SSF81606. PP2C-related. 1 hit.
PROSITE	PS01032. PP2C. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	970748.

Entry information

Entry name

PP2C3_YEAST

Accession

Primary (citable) accession number: P34221
Secondary accession number(s): D6VPU4, Q92330

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1994
Last sequence update:	July 27, 2011
Last modified:	May 29, 2013
This is version 122 of the entry and version 4 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program