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P34221

- PP2C3_YEAST

UniProt

P34221 - PP2C3_YEAST

Protein

Protein phosphatase 2C homolog 3

Gene

PTC3

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 131 (01 Oct 2014)
      Sequence version 4 (27 Jul 2011)
      Previous versions | rss
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    Functioni

    Responsible, together with PTC2, for the dephosphorylation of the cyclin-dependent protein kinase CDC28.1 Publication

    Catalytic activityi

    [a protein]-serine/threonine phosphate + H2O = [a protein]-serine/threonine + phosphate.

    Cofactori

    Binds 2 magnesium or manganese ions per subunit.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi62 – 621Manganese 1By similarity
    Metal bindingi62 – 621Manganese 2By similarity
    Metal bindingi63 – 631Manganese 1; via carbonyl oxygenBy similarity
    Metal bindingi234 – 2341Manganese 2By similarity
    Metal bindingi284 – 2841Manganese 2By similarity

    GO - Molecular functioni

    1. metal ion binding Source: UniProtKB-KW
    2. protein binding Source: IntAct
    3. protein serine/threonine phosphatase activity Source: SGD

    GO - Biological processi

    1. inactivation of MAPK activity involved in osmosensory signaling pathway Source: SGD
    2. protein dephosphorylation Source: SGD
    3. regulation of cyclin-dependent protein serine/threonine kinase activity Source: SGD

    Keywords - Molecular functioni

    Hydrolase, Protein phosphatase

    Keywords - Ligandi

    Magnesium, Manganese, Metal-binding

    Enzyme and pathway databases

    BioCyciYEAST:G3O-28954-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Protein phosphatase 2C homolog 3 (EC:3.1.3.16)
    Short name:
    PP2C-3
    Gene namesi
    Name:PTC3
    Ordered Locus Names:YBL056W
    ORF Names:YBL0511, YBL0513
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome II

    Organism-specific databases

    CYGDiYBL056w.
    SGDiS000000152. PTC3.

    Subcellular locationi

    Cytoplasm 1 Publication. Nucleus 1 Publication

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell
    2. nucleus Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm, Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 468468Protein phosphatase 2C homolog 3PRO_0000057776Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei324 – 3241Phosphoserine2 Publications
    Modified residuei332 – 3321Phosphoserine1 Publication

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiP34221.
    PaxDbiP34221.
    PeptideAtlasiP34221.

    Expressioni

    Gene expression databases

    GenevestigatoriP34221.

    Interactioni

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    PAA1Q124476EBI-12805,EBI-33397

    Protein-protein interaction databases

    BioGridi32643. 46 interactions.
    DIPiDIP-3944N.
    IntActiP34221. 20 interactions.
    MINTiMINT-514472.
    STRINGi4932.YBL056W.

    Structurei

    3D structure databases

    ProteinModelPortaliP34221.
    SMRiP34221. Positions 1-275.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the PP2C family.Curated

    Phylogenomic databases

    eggNOGiCOG0631.
    GeneTreeiENSGT00740000115384.
    HOGENOMiHOG000233896.
    KOiK14803.
    OrthoDBiEOG79930T.

    Family and domain databases

    Gene3Di3.60.40.10. 1 hit.
    InterProiIPR001932. PP2C-like_dom.
    IPR000222. PP2C_Mn2_Asp60_BS.
    IPR015655. Protein_Pase_2C.
    [Graphical view]
    PANTHERiPTHR13832. PTHR13832. 1 hit.
    PfamiPF00481. PP2C. 1 hit.
    [Graphical view]
    SMARTiSM00331. PP2C_SIG. 1 hit.
    SM00332. PP2Cc. 1 hit.
    [Graphical view]
    SUPFAMiSSF81606. SSF81606. 1 hit.
    PROSITEiPS01032. PP2C. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P34221-1 [UniParc]FASTAAdd to Basket

    « Hide

    MGQILSNPII DKEHHSGTDC LTAFGLCAMQ GWRMSMEDAH IVEPNLLAES    50
    DEEHLAFYGI FDGHGGSSVA EFCGSKMISI LKKQESFKSG MLEQCLIDTF 100
    LATDVELLKD EKLKDDHSGC TATVILVSQL KKLLICANSG DSRTVLSTGG 150
    NSKAMSFDHK PTLLSEKSRI VAADGFVEMD RVNGNLALSR AIGDFEFKSN 200
    TKLGPHEQVV TCVPDIICHN LNYDEDEFVI LACDGIWDCL TSQECVDLVH 250
    YGISQGNMTL SDISSRIVDV CCSPTTEGSG IGCDNMSISI VALLKENESE 300
    SQWFERMRSK NYNIQTSFVQ RRKSIFDFHD FSDDDNEVFA ITTKKLQDRL 350
    NRSKDNDDME IDDLDTELGS SATPSKLSGE DRTGPIDLFS LEALLEAGIQ 400
    IRQRPSSDSD GNTSYFHGAS LSDMLASLSN AAAGETEPND ADDNDDNDGE 450
    ENGKNENAKK GSKIEEIE 468
    Length:468
    Mass (Da):51,332
    Last modified:July 27, 2011 - v4
    Checksum:i70AC2707C7FA2122
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti369 – 3691G → D in CAA80791. (PubMed:8154187)Curated
    Sequence conflicti369 – 3691G → D in CAA84876. (PubMed:7813418)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U72346 Genomic DNA. Translation: AAB17351.1.
    Z23261 Genomic DNA. Translation: CAA80791.1.
    Z35817 Genomic DNA. Translation: CAA84876.1.
    AY692754 Genomic DNA. Translation: AAT92773.1.
    BK006936 Genomic DNA. Translation: DAA07064.2.
    PIRiS39832.
    RefSeqiNP_009497.2. NM_001178296.2.

    Genome annotation databases

    EnsemblFungiiYBL056W; YBL056W; YBL056W.
    GeneIDi852224.
    KEGGisce:YBL056W.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U72346 Genomic DNA. Translation: AAB17351.1 .
    Z23261 Genomic DNA. Translation: CAA80791.1 .
    Z35817 Genomic DNA. Translation: CAA84876.1 .
    AY692754 Genomic DNA. Translation: AAT92773.1 .
    BK006936 Genomic DNA. Translation: DAA07064.2 .
    PIRi S39832.
    RefSeqi NP_009497.2. NM_001178296.2.

    3D structure databases

    ProteinModelPortali P34221.
    SMRi P34221. Positions 1-275.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 32643. 46 interactions.
    DIPi DIP-3944N.
    IntActi P34221. 20 interactions.
    MINTi MINT-514472.
    STRINGi 4932.YBL056W.

    Proteomic databases

    MaxQBi P34221.
    PaxDbi P34221.
    PeptideAtlasi P34221.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YBL056W ; YBL056W ; YBL056W .
    GeneIDi 852224.
    KEGGi sce:YBL056W.

    Organism-specific databases

    CYGDi YBL056w.
    SGDi S000000152. PTC3.

    Phylogenomic databases

    eggNOGi COG0631.
    GeneTreei ENSGT00740000115384.
    HOGENOMi HOG000233896.
    KOi K14803.
    OrthoDBi EOG79930T.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-28954-MONOMER.

    Miscellaneous databases

    NextBioi 970748.

    Gene expression databases

    Genevestigatori P34221.

    Family and domain databases

    Gene3Di 3.60.40.10. 1 hit.
    InterProi IPR001932. PP2C-like_dom.
    IPR000222. PP2C_Mn2_Asp60_BS.
    IPR015655. Protein_Pase_2C.
    [Graphical view ]
    PANTHERi PTHR13832. PTHR13832. 1 hit.
    Pfami PF00481. PP2C. 1 hit.
    [Graphical view ]
    SMARTi SM00331. PP2C_SIG. 1 hit.
    SM00332. PP2Cc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF81606. SSF81606. 1 hit.
    PROSITEi PS01032. PP2C. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Maeda T., Wurlger-Murphy S.M., Saito H.
      Submitted (SEP-1996) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. "Sequencing and functional analysis of a 32,560 bp segment on the left arm of yeast chromosome II. Identification of 26 open reading frames, including the KIP1 and SEC17 genes."
      Scherens B., el Bakkoury M., Vierendeels F., Dubois E., Messenguy F.
      Yeast 9:1355-1371(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    3. "Complete DNA sequence of yeast chromosome II."
      Feldmann H., Aigle M., Aljinovic G., Andre B., Baclet M.C., Barthe C., Baur A., Becam A.-M., Biteau N., Boles E., Brandt T., Brendel M., Brueckner M., Bussereau F., Christiansen C., Contreras R., Crouzet M., Cziepluch C.
      , Demolis N., Delaveau T., Doignon F., Domdey H., Duesterhus S., Dubois E., Dujon B., El Bakkoury M., Entian K.-D., Feuermann M., Fiers W., Fobo G.M., Fritz C., Gassenhuber J., Glansdorff N., Goffeau A., Grivell L.A., de Haan M., Hein C., Herbert C.J., Hollenberg C.P., Holmstroem K., Jacq C., Jacquet M., Jauniaux J.-C., Jonniaux J.-L., Kallesoee T., Kiesau P., Kirchrath L., Koetter P., Korol S., Liebl S., Logghe M., Lohan A.J.E., Louis E.J., Li Z.Y., Maat M.J., Mallet L., Mannhaupt G., Messenguy F., Miosga T., Molemans F., Mueller S., Nasr F., Obermaier B., Perea J., Pierard A., Piravandi E., Pohl F.M., Pohl T.M., Potier S., Proft M., Purnelle B., Ramezani Rad M., Rieger M., Rose M., Schaaff-Gerstenschlaeger I., Scherens B., Schwarzlose C., Skala J., Slonimski P.P., Smits P.H.M., Souciet J.-L., Steensma H.Y., Stucka R., Urrestarazu L.A., van der Aart Q.J.M., Van Dyck L., Vassarotti A., Vetter I., Vierendeels F., Vissers S., Wagner G., de Wergifosse P., Wolfe K.H., Zagulski M., Zimmermann F.K., Mewes H.-W., Kleine K.
      EMBO J. 13:5795-5809(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    4. Cited for: GENOME REANNOTATION, SEQUENCE REVISION TO 369.
      Strain: ATCC 204508 / S288c.
    5. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    6. "Dephosphorylation of cyclin-dependent kinases by type 2C protein phosphatases."
      Cheng A., Ross K.E., Kaldis P., Solomon M.J.
      Genes Dev. 13:2946-2957(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    7. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
    8. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    9. "Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
      Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
      J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-324 AND SER-332, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Strain: ADR376.
    10. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
      Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
      Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-324, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiPP2C3_YEAST
    AccessioniPrimary (citable) accession number: P34221
    Secondary accession number(s): D6VPU4, Q92330
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1994
    Last sequence update: July 27, 2011
    Last modified: October 1, 2014
    This is version 131 of the entry and version 4 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 19700 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    3. Yeast chromosome II
      Yeast (Saccharomyces cerevisiae) chromosome II: entries and gene names

    External Data

    Dasty 3