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P34221

- PP2C3_YEAST

UniProt

P34221 - PP2C3_YEAST

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Protein

Protein phosphatase 2C homolog 3

Gene

PTC3

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Responsible, together with PTC2, for the dephosphorylation of the cyclin-dependent protein kinase CDC28.1 Publication

Catalytic activityi

[a protein]-serine/threonine phosphate + H2O = [a protein]-serine/threonine + phosphate.

Cofactori

Binds 2 magnesium or manganese ions per subunit.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi62 – 621Manganese 1By similarity
Metal bindingi62 – 621Manganese 2By similarity
Metal bindingi63 – 631Manganese 1; via carbonyl oxygenBy similarity
Metal bindingi234 – 2341Manganese 2By similarity
Metal bindingi284 – 2841Manganese 2By similarity

GO - Molecular functioni

  1. metal ion binding Source: UniProtKB-KW
  2. protein serine/threonine phosphatase activity Source: SGD

GO - Biological processi

  1. inactivation of MAPK activity involved in osmosensory signaling pathway Source: SGD
  2. protein dephosphorylation Source: SGD
  3. regulation of cyclin-dependent protein serine/threonine kinase activity Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protein phosphatase

Keywords - Ligandi

Magnesium, Manganese, Metal-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-28954-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Protein phosphatase 2C homolog 3 (EC:3.1.3.16)
Short name:
PP2C-3
Gene namesi
Name:PTC3
Ordered Locus Names:YBL056W
ORF Names:YBL0511, YBL0513
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome II

Organism-specific databases

CYGDiYBL056w.
SGDiS000000152. PTC3.

Subcellular locationi

Cytoplasm 1 Publication. Nucleus 1 Publication

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-KW
  2. nucleus Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 468468Protein phosphatase 2C homolog 3PRO_0000057776Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei324 – 3241Phosphoserine2 Publications
Modified residuei332 – 3321Phosphoserine1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP34221.
PaxDbiP34221.
PeptideAtlasiP34221.

Expressioni

Gene expression databases

GenevestigatoriP34221.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
PAA1Q124476EBI-12805,EBI-33397

Protein-protein interaction databases

BioGridi32643. 49 interactions.
DIPiDIP-3944N.
IntActiP34221. 20 interactions.
MINTiMINT-514472.
STRINGi4932.YBL056W.

Structurei

3D structure databases

ProteinModelPortaliP34221.
SMRiP34221. Positions 1-275.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the PP2C family.Curated

Phylogenomic databases

eggNOGiCOG0631.
GeneTreeiENSGT00740000115384.
HOGENOMiHOG000233896.
KOiK14803.
OrthoDBiEOG79930T.

Family and domain databases

Gene3Di3.60.40.10. 1 hit.
InterProiIPR001932. PP2C-like_dom.
IPR000222. PP2C_Mn2_Asp60_BS.
IPR015655. Protein_Pase_2C.
[Graphical view]
PANTHERiPTHR13832. PTHR13832. 1 hit.
PfamiPF00481. PP2C. 1 hit.
[Graphical view]
SMARTiSM00331. PP2C_SIG. 1 hit.
SM00332. PP2Cc. 1 hit.
[Graphical view]
SUPFAMiSSF81606. SSF81606. 1 hit.
PROSITEiPS01032. PP2C. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P34221-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MGQILSNPII DKEHHSGTDC LTAFGLCAMQ GWRMSMEDAH IVEPNLLAES
60 70 80 90 100
DEEHLAFYGI FDGHGGSSVA EFCGSKMISI LKKQESFKSG MLEQCLIDTF
110 120 130 140 150
LATDVELLKD EKLKDDHSGC TATVILVSQL KKLLICANSG DSRTVLSTGG
160 170 180 190 200
NSKAMSFDHK PTLLSEKSRI VAADGFVEMD RVNGNLALSR AIGDFEFKSN
210 220 230 240 250
TKLGPHEQVV TCVPDIICHN LNYDEDEFVI LACDGIWDCL TSQECVDLVH
260 270 280 290 300
YGISQGNMTL SDISSRIVDV CCSPTTEGSG IGCDNMSISI VALLKENESE
310 320 330 340 350
SQWFERMRSK NYNIQTSFVQ RRKSIFDFHD FSDDDNEVFA ITTKKLQDRL
360 370 380 390 400
NRSKDNDDME IDDLDTELGS SATPSKLSGE DRTGPIDLFS LEALLEAGIQ
410 420 430 440 450
IRQRPSSDSD GNTSYFHGAS LSDMLASLSN AAAGETEPND ADDNDDNDGE
460
ENGKNENAKK GSKIEEIE
Length:468
Mass (Da):51,332
Last modified:July 27, 2011 - v4
Checksum:i70AC2707C7FA2122
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti369 – 3691G → D in CAA80791. (PubMed:8154187)Curated
Sequence conflicti369 – 3691G → D in CAA84876. (PubMed:7813418)Curated

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U72346 Genomic DNA. Translation: AAB17351.1.
Z23261 Genomic DNA. Translation: CAA80791.1.
Z35817 Genomic DNA. Translation: CAA84876.1.
AY692754 Genomic DNA. Translation: AAT92773.1.
BK006936 Genomic DNA. Translation: DAA07064.2.
PIRiS39832.
RefSeqiNP_009497.2. NM_001178296.2.

Genome annotation databases

EnsemblFungiiYBL056W; YBL056W; YBL056W.
GeneIDi852224.
KEGGisce:YBL056W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U72346 Genomic DNA. Translation: AAB17351.1 .
Z23261 Genomic DNA. Translation: CAA80791.1 .
Z35817 Genomic DNA. Translation: CAA84876.1 .
AY692754 Genomic DNA. Translation: AAT92773.1 .
BK006936 Genomic DNA. Translation: DAA07064.2 .
PIRi S39832.
RefSeqi NP_009497.2. NM_001178296.2.

3D structure databases

ProteinModelPortali P34221.
SMRi P34221. Positions 1-275.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 32643. 49 interactions.
DIPi DIP-3944N.
IntActi P34221. 20 interactions.
MINTi MINT-514472.
STRINGi 4932.YBL056W.

Proteomic databases

MaxQBi P34221.
PaxDbi P34221.
PeptideAtlasi P34221.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YBL056W ; YBL056W ; YBL056W .
GeneIDi 852224.
KEGGi sce:YBL056W.

Organism-specific databases

CYGDi YBL056w.
SGDi S000000152. PTC3.

Phylogenomic databases

eggNOGi COG0631.
GeneTreei ENSGT00740000115384.
HOGENOMi HOG000233896.
KOi K14803.
OrthoDBi EOG79930T.

Enzyme and pathway databases

BioCyci YEAST:G3O-28954-MONOMER.

Miscellaneous databases

NextBioi 970748.

Gene expression databases

Genevestigatori P34221.

Family and domain databases

Gene3Di 3.60.40.10. 1 hit.
InterProi IPR001932. PP2C-like_dom.
IPR000222. PP2C_Mn2_Asp60_BS.
IPR015655. Protein_Pase_2C.
[Graphical view ]
PANTHERi PTHR13832. PTHR13832. 1 hit.
Pfami PF00481. PP2C. 1 hit.
[Graphical view ]
SMARTi SM00331. PP2C_SIG. 1 hit.
SM00332. PP2Cc. 1 hit.
[Graphical view ]
SUPFAMi SSF81606. SSF81606. 1 hit.
PROSITEi PS01032. PP2C. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Maeda T., Wurlger-Murphy S.M., Saito H.
    Submitted (SEP-1996) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "Sequencing and functional analysis of a 32,560 bp segment on the left arm of yeast chromosome II. Identification of 26 open reading frames, including the KIP1 and SEC17 genes."
    Scherens B., el Bakkoury M., Vierendeels F., Dubois E., Messenguy F.
    Yeast 9:1355-1371(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. "Complete DNA sequence of yeast chromosome II."
    Feldmann H., Aigle M., Aljinovic G., Andre B., Baclet M.C., Barthe C., Baur A., Becam A.-M., Biteau N., Boles E., Brandt T., Brendel M., Brueckner M., Bussereau F., Christiansen C., Contreras R., Crouzet M., Cziepluch C.
    , Demolis N., Delaveau T., Doignon F., Domdey H., Duesterhus S., Dubois E., Dujon B., El Bakkoury M., Entian K.-D., Feuermann M., Fiers W., Fobo G.M., Fritz C., Gassenhuber J., Glansdorff N., Goffeau A., Grivell L.A., de Haan M., Hein C., Herbert C.J., Hollenberg C.P., Holmstroem K., Jacq C., Jacquet M., Jauniaux J.-C., Jonniaux J.-L., Kallesoee T., Kiesau P., Kirchrath L., Koetter P., Korol S., Liebl S., Logghe M., Lohan A.J.E., Louis E.J., Li Z.Y., Maat M.J., Mallet L., Mannhaupt G., Messenguy F., Miosga T., Molemans F., Mueller S., Nasr F., Obermaier B., Perea J., Pierard A., Piravandi E., Pohl F.M., Pohl T.M., Potier S., Proft M., Purnelle B., Ramezani Rad M., Rieger M., Rose M., Schaaff-Gerstenschlaeger I., Scherens B., Schwarzlose C., Skala J., Slonimski P.P., Smits P.H.M., Souciet J.-L., Steensma H.Y., Stucka R., Urrestarazu L.A., van der Aart Q.J.M., Van Dyck L., Vassarotti A., Vetter I., Vierendeels F., Vissers S., Wagner G., de Wergifosse P., Wolfe K.H., Zagulski M., Zimmermann F.K., Mewes H.-W., Kleine K.
    EMBO J. 13:5795-5809(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  4. Cited for: GENOME REANNOTATION, SEQUENCE REVISION TO 369.
    Strain: ATCC 204508 / S288c.
  5. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  6. "Dephosphorylation of cyclin-dependent kinases by type 2C protein phosphatases."
    Cheng A., Ross K.E., Kaldis P., Solomon M.J.
    Genes Dev. 13:2946-2957(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  7. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
  8. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  9. "Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
    Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
    J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-324 AND SER-332, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Strain: ADR376.
  10. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
    Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
    Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-324, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiPP2C3_YEAST
AccessioniPrimary (citable) accession number: P34221
Secondary accession number(s): D6VPU4, Q92330
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: July 27, 2011
Last modified: October 29, 2014
This is version 132 of the entry and version 4 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 19700 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome II
    Yeast (Saccharomyces cerevisiae) chromosome II: entries and gene names

External Data

Dasty 3