Reviewed, UniProtKB/Swiss-Prot P34220 (YBF5_YEAST)

Names and origin

Protein names

Recommended name:
    Deoxyribonuclease Tat-D
    EC=3.1.21.-

Gene names

Ordered Locus Names:	YBL055C
ORF Names:	YBL0512, YBL0511

Organism

Saccharomyces cerevisiae (Baker's yeast) [Complete proteome]

Taxonomic identifier

4932 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	418 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Has both endo- and exonuclease activities. Incises double-stranded DNA without obvious specificity via its endonuclease activity and excises the DNA from the 3'-to 5'-end by its exonuclease activity. May have a role in apoptosis. Ref.6
Cofactor	Divalent metal cations. Has optimal activity with magnesium. Ref.6
Subcellular location	Cytoplasm. Ref.4
Miscellaneous	Present with 1460 molecules/cell in log phase SD medium. Ref.5
Sequence similarities	Belongs to the tatD DNase family.
Biophysicochemical properties	pH dependence: Optimum pH is 5. Temperature dependence: Optimum temperature is 30 degrees Celsius.

Ontologies

Keywords
Cellular component	Cytoplasm
Molecular function	Hydrolase Nuclease
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	DNA fragmentation involved in apoptosis Ref.6 Inferred from mutant phenotype. Source: SGD response to oxidative stress Ref.6 Inferred from mutant phenotype. Source: SGD
Cellular component	cytoplasm Ref.4 Inferred from direct assay. Source: SGD
Molecular function	3'-5'-exodeoxyribonuclease activity Ref.6 Inferred from direct assay. Source: SGD endodeoxyribonuclease activity, producing 5'-phosphomonoesters Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 418	418	Deoxyribonuclease Tat-D		PRO_0000201994
Experimental info
	Mutagenesis	185	1	E → A: Reduces enzymatic activities by 50%. Ref.6
	Mutagenesis	325	1	E → A: Reduces enzymatic activities by 50%. Ref.6
	Mutagenesis	327	1	D → A: Reduces enzymatic activities by almost 95%. Ref.6
	Sequence conflict	278	1	S → P in AAT92645. Ref.3

Sequences

	Sequence		Length	Mass (Da)
	P34220-1 [UniParc]. Last modified February 1, 1994. Version 1. Checksum: F1107C1B8E94CAD1	FASTA	418	47,390
	10 20 30 40 50 60 MWGILLKSSN KSCSRLWKPI LTQYYSMTST ATDSPLKYYD IGLNLTDPMF HGIYNGKQYH 70 80 90 100 110 120 PADYVKLLER AAQRHVKNAL VTGSSIAESQ SAIELVSSVK DLSPLKLYHT IGVHPCCVNE 130 140 150 160 170 180 FADASQGDKA SASIDNPSMD EAYNESLYAK VISNPSFAQG KLKELYDLMN QQAKPHDTSF 190 200 210 220 230 240 RSIGEIGLDY DRFHYSSKEM QKVFFEEQLK ISCLNDKLSS YPLFLHMRSA CDDFVQILER 250 260 270 280 290 300 FIAGFTDERD TFQLQKLGAS SSSGFYKFHP DRKLVVHSFT GSAIDLQKLL NLSPNIFIGV 310 320 330 340 350 360 NGCSLRTEEN LAVVKQIPTE RLLLETDAPW CEIKRTHASF QYLAKYQEVR DFEYPAFKSV 370 380 390 400 410 KKNKLADKLN AEELYMVKGR NEPCNMEQVA IVVSEVKDVD LATLIDTTWK TTCKIFGE

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequencing and functional analysis of a 32,560 bp segment on the left arm of yeast chromosome II. Identification of 26 open reading frames, including the KIP1 and SEC17 genes." Scherens B., el Bakkoury M., Vierendeels F., Dubois E., Messenguy F. Yeast 9:1355-1371(1993) [PubMed: 8154187] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[2]	"Complete DNA sequence of yeast chromosome II." Feldmann H., Aigle M., Aljinovic G., Andre B., Baclet M.C., Barthe C., Baur A., Becam A.-M., Biteau N., Boles E., Brandt T., Brendel M., Brueckner M., Bussereau F., Christiansen C., Contreras R., Crouzet M., Cziepluch C. , Demolis N., Delaveau T., Doignon F., Domdey H., Duesterhus S., Dubois E., Dujon B., El Bakkoury M., Entian K.-D., Feuermann M., Fiers W., Fobo G.M., Fritz C., Gassenhuber J., Glansdorff N., Goffeau A., Grivell L.A., de Haan M., Hein C., Herbert C.J., Hollenberg C.P., Holmstroem K., Jacq C., Jacquet M., Jauniaux J.-C., Jonniaux J.-L., Kallesoee T., Kiesau P., Kirchrath L., Koetter P., Korol S., Liebl S., Logghe M., Lohan A.J.E., Louis E.J., Li Z.Y., Maat M.J., Mallet L., Mannhaupt G., Messenguy F., Miosga T., Molemans F., Mueller S., Nasr F., Obermaier B., Perea J., Pierard A., Piravandi E., Pohl F.M., Pohl T.M., Potier S., Proft M., Purnelle B., Ramezani Rad M., Rieger M., Rose M., Schaaff-Gerstenschlaeger I., Scherens B., Schwarzlose C., Skala J., Slonimski P.P., Smits P.H.M., Souciet J.-L., Steensma H.Y., Stucka R., Urrestarazu L.A., van der Aart Q.J.M., Van Dyck L., Vassarotti A., Vetter I., Vierendeels F., Vissers S., Wagner G., de Wergifosse P., Wolfe K.H., Zagulski M., Zimmermann F.K., Mewes H.-W., Kleine K. EMBO J. 13:5795-5809(1994) [PubMed: 7813418] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[3]	"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae." Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J. Genome Res. 17:536-543(2007) [PubMed: 17322287] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[4]	"Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed: 14562095] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[5]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[6]	"Search for apoptotic nucleases in yeast: role of Tat-D nuclease in apoptotic DNA degradation." Qiu J., Yoon J.H., Shen B. J. Biol. Chem. 280:15370-15379(2005) [PubMed: 15657035] [Abstract] Cited for: FUNCTION, COFACTOR, BIOPHYSICOCHEMICAL PROPERTIES, MUTAGENESIS OF GLU-185; GLU-325 AND ASP-327.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	Z23261 Genomic DNA. Translation: CAA80792.1. Z35816 Genomic DNA. Translation: CAA84875.1. AY692626 Genomic DNA. Translation: AAT92645.1.
PIR	S39833.
RefSeq	NP_009498.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	P34220.
Proteomic databases
PeptideAtlas	P34220.
Genome annotation databases
Ensembl	YBL055C; YBL055C; YBL055C; Saccharomyces cerevisiae. [Genome view]
GeneID	852225.
GenomeReviews	Gene locus YBL055C in contig Y13134_GR.
KEGG	sce:YBL055C.
NMPDR	fig|4932.3.peg.188.
Organism-specific databases
CYGD	YBL055c.
SGD	S000000151. YBL055C.
Phylogenomic databases
HOGENOM	P34220.
OMA	DIGVNFS.
Gene expression databases
ArrayExpress	P34220.
Genevestigator	P34220.
GermOnline	YBL055C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR015992. DNase_TatD. IPR001130. DNase_TatD-rel. IPR018228. DNase_TatD-rel_CS. [Graphical view]
PANTHER	PTHR10060. TatD_DNase. 1 hit.
Pfam	PF01026. TatD_DNase. 1 hit. [Graphical view]
PROSITE	PS01137. TATD_1. False negative. PS01090. TATD_2. 1 hit. PS01091. TATD_3. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	970751.

Entry information

Entry name

YBF5_YEAST

Accession

Primary (citable) accession number: P34220
Secondary accession number(s): Q6B2V4

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1994
Last sequence update:	February 1, 1994
Last modified:	November 3, 2009
This is version 60 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)