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P34220

- YBF5_YEAST

UniProt

P34220 - YBF5_YEAST

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Protein

Deoxyribonuclease Tat-D

Gene
YBL055C, YBL0511, YBL0512
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Has both endo- and exonuclease activities. Incises double-stranded DNA without obvious specificity via its endonuclease activity and excises the DNA from the 3'-to 5'-end by its exonuclease activity. May have a role in apoptosis.1 Publication

Cofactori

Divalent metal cations. Has optimal activity with magnesium.1 Publication

pH dependencei

Optimum pH is 5.1 Publication

Temperature dependencei

Optimum temperature is 30 degrees Celsius.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi185 – 1851Divalent metal cation 1 By similarity
Metal bindingi185 – 1851Divalent metal cation 2 By similarity
Metal bindingi226 – 2261Divalent metal cation 2 By similarity
Metal bindingi277 – 2771Divalent metal cation 2 By similarity
Metal bindingi327 – 3271Divalent metal cation 1 By similarity

GO - Molecular functioni

  1. 3'-5'-exodeoxyribonuclease activity Source: SGD
  2. endodeoxyribonuclease activity, producing 5'-phosphomonoesters Source: InterPro
  3. endonuclease activity Source: SGD
  4. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. apoptotic DNA fragmentation Source: SGD
  2. cellular response to oxidative stress Source: SGD
  3. DNA catabolic process, exonucleolytic Source: GOC
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Nuclease

Keywords - Ligandi

Metal-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-28953-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Deoxyribonuclease Tat-D (EC:3.1.21.-)
Gene namesi
Ordered Locus Names:YBL055C
ORF Names:YBL0511, YBL0512
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome II

Organism-specific databases

CYGDiYBL055c.
SGDiS000000151. YBL055C.

Subcellular locationi

Cytoplasm 1 Publication

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi185 – 1851E → A: Reduces enzymatic activities by 50%. 1 Publication
Mutagenesisi325 – 3251E → A: Reduces enzymatic activities by 50%. 1 Publication
Mutagenesisi327 – 3271D → A: Reduces enzymatic activities by almost 95%. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 418418Deoxyribonuclease Tat-DPRO_0000201994Add
BLAST

Proteomic databases

MaxQBiP34220.
PaxDbiP34220.
PeptideAtlasiP34220.

Expressioni

Gene expression databases

GenevestigatoriP34220.

Interactioni

Protein-protein interaction databases

BioGridi32644. 24 interactions.
MINTiMINT-2492113.
STRINGi4932.YBL055C.

Structurei

3D structure databases

ProteinModelPortaliP34220.
SMRiP34220. Positions 35-417.

Family & Domainsi

Sequence similaritiesi

Belongs to the TatD DNase family.

Phylogenomic databases

eggNOGiCOG0084.
GeneTreeiENSGT00720000108776.
HOGENOMiHOG000201523.
KOiK03424.
OMAiRYIDIGI.
OrthoDBiEOG7PZS7D.

Family and domain databases

InterProiIPR018228. DNase_TatD-rel_CS.
IPR001130. TatD_family.
[Graphical view]
PANTHERiPTHR10060. PTHR10060. 1 hit.
PfamiPF01026. TatD_DNase. 1 hit.
[Graphical view]
PROSITEiPS01090. TATD_2. 1 hit.
PS01091. TATD_3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P34220-1 [UniParc]FASTAAdd to Basket

« Hide

MWGILLKSSN KSCSRLWKPI LTQYYSMTST ATDSPLKYYD IGLNLTDPMF    50
HGIYNGKQYH PADYVKLLER AAQRHVKNAL VTGSSIAESQ SAIELVSSVK 100
DLSPLKLYHT IGVHPCCVNE FADASQGDKA SASIDNPSMD EAYNESLYAK 150
VISNPSFAQG KLKELYDLMN QQAKPHDTSF RSIGEIGLDY DRFHYSSKEM 200
QKVFFEEQLK ISCLNDKLSS YPLFLHMRSA CDDFVQILER FIAGFTDERD 250
TFQLQKLGAS SSSGFYKFHP DRKLVVHSFT GSAIDLQKLL NLSPNIFIGV 300
NGCSLRTEEN LAVVKQIPTE RLLLETDAPW CEIKRTHASF QYLAKYQEVR 350
DFEYPAFKSV KKNKLADKLN AEELYMVKGR NEPCNMEQVA IVVSEVKDVD 400
LATLIDTTWK TTCKIFGE 418
Length:418
Mass (Da):47,390
Last modified:February 1, 1994 - v1
Checksum:iF1107C1B8E94CAD1
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti278 – 2781S → P in AAT92645. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Z23261 Genomic DNA. Translation: CAA80792.1.
Z35816 Genomic DNA. Translation: CAA84875.1.
AY692626 Genomic DNA. Translation: AAT92645.1.
BK006936 Genomic DNA. Translation: DAA07065.1.
PIRiS39833.
RefSeqiNP_009498.1. NM_001178295.1.

Genome annotation databases

EnsemblFungiiYBL055C; YBL055C; YBL055C.
GeneIDi852225.
KEGGisce:YBL055C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Z23261 Genomic DNA. Translation: CAA80792.1 .
Z35816 Genomic DNA. Translation: CAA84875.1 .
AY692626 Genomic DNA. Translation: AAT92645.1 .
BK006936 Genomic DNA. Translation: DAA07065.1 .
PIRi S39833.
RefSeqi NP_009498.1. NM_001178295.1.

3D structure databases

ProteinModelPortali P34220.
SMRi P34220. Positions 35-417.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 32644. 24 interactions.
MINTi MINT-2492113.
STRINGi 4932.YBL055C.

Proteomic databases

MaxQBi P34220.
PaxDbi P34220.
PeptideAtlasi P34220.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YBL055C ; YBL055C ; YBL055C .
GeneIDi 852225.
KEGGi sce:YBL055C.

Organism-specific databases

CYGDi YBL055c.
SGDi S000000151. YBL055C.

Phylogenomic databases

eggNOGi COG0084.
GeneTreei ENSGT00720000108776.
HOGENOMi HOG000201523.
KOi K03424.
OMAi RYIDIGI.
OrthoDBi EOG7PZS7D.

Enzyme and pathway databases

BioCyci YEAST:G3O-28953-MONOMER.

Miscellaneous databases

NextBioi 970751.
PROi P34220.

Gene expression databases

Genevestigatori P34220.

Family and domain databases

InterProi IPR018228. DNase_TatD-rel_CS.
IPR001130. TatD_family.
[Graphical view ]
PANTHERi PTHR10060. PTHR10060. 1 hit.
Pfami PF01026. TatD_DNase. 1 hit.
[Graphical view ]
PROSITEi PS01090. TATD_2. 1 hit.
PS01091. TATD_3. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Sequencing and functional analysis of a 32,560 bp segment on the left arm of yeast chromosome II. Identification of 26 open reading frames, including the KIP1 and SEC17 genes."
    Scherens B., el Bakkoury M., Vierendeels F., Dubois E., Messenguy F.
    Yeast 9:1355-1371(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  2. "Complete DNA sequence of yeast chromosome II."
    Feldmann H., Aigle M., Aljinovic G., Andre B., Baclet M.C., Barthe C., Baur A., Becam A.-M., Biteau N., Boles E., Brandt T., Brendel M., Brueckner M., Bussereau F., Christiansen C., Contreras R., Crouzet M., Cziepluch C.
    , Demolis N., Delaveau T., Doignon F., Domdey H., Duesterhus S., Dubois E., Dujon B., El Bakkoury M., Entian K.-D., Feuermann M., Fiers W., Fobo G.M., Fritz C., Gassenhuber J., Glansdorff N., Goffeau A., Grivell L.A., de Haan M., Hein C., Herbert C.J., Hollenberg C.P., Holmstroem K., Jacq C., Jacquet M., Jauniaux J.-C., Jonniaux J.-L., Kallesoee T., Kiesau P., Kirchrath L., Koetter P., Korol S., Liebl S., Logghe M., Lohan A.J.E., Louis E.J., Li Z.Y., Maat M.J., Mallet L., Mannhaupt G., Messenguy F., Miosga T., Molemans F., Mueller S., Nasr F., Obermaier B., Perea J., Pierard A., Piravandi E., Pohl F.M., Pohl T.M., Potier S., Proft M., Purnelle B., Ramezani Rad M., Rieger M., Rose M., Schaaff-Gerstenschlaeger I., Scherens B., Schwarzlose C., Skala J., Slonimski P.P., Smits P.H.M., Souciet J.-L., Steensma H.Y., Stucka R., Urrestarazu L.A., van der Aart Q.J.M., Van Dyck L., Vassarotti A., Vetter I., Vierendeels F., Vissers S., Wagner G., de Wergifosse P., Wolfe K.H., Zagulski M., Zimmermann F.K., Mewes H.-W., Kleine K.
    EMBO J. 13:5795-5809(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  4. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  5. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
  6. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  7. "Search for apoptotic nucleases in yeast: role of Tat-D nuclease in apoptotic DNA degradation."
    Qiu J., Yoon J.H., Shen B.
    J. Biol. Chem. 280:15370-15379(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, COFACTOR, BIOPHYSICOCHEMICAL PROPERTIES, MUTAGENESIS OF GLU-185; GLU-325 AND ASP-327.

Entry informationi

Entry nameiYBF5_YEAST
AccessioniPrimary (citable) accession number: P34220
Secondary accession number(s): D6VPU5, Q6B2V4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: February 1, 1994
Last modified: May 14, 2014
This is version 99 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 1460 molecules/cell in log phase SD medium.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome II
    Yeast (Saccharomyces cerevisiae) chromosome II: entries and gene names

External Data

Dasty 3

Similar proteinsi