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Protein

Deoxyribonuclease Tat-D

Gene

YBL055C

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Has both endo- and exonuclease activities. Incises double-stranded DNA without obvious specificity via its endonuclease activity and excises the DNA from the 3'-to 5'-end by its exonuclease activity. May have a role in apoptosis.1 Publication

Cofactori

Mg2+1 PublicationNote: Divalent metal cations. Has optimal activity with Mg2+.1 Publication

pH dependencei

Optimum pH is 5.1 Publication

Temperature dependencei

Optimum temperature is 30 degrees Celsius.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi185Divalent metal cation 1By similarity1
Metal bindingi185Divalent metal cation 2By similarity1
Metal bindingi226Divalent metal cation 2By similarity1
Metal bindingi277Divalent metal cation 2By similarity1
Metal bindingi327Divalent metal cation 1By similarity1

GO - Molecular functioni

  • 3'-5'-exodeoxyribonuclease activity Source: SGD
  • endodeoxyribonuclease activity, producing 5'-phosphomonoesters Source: InterPro
  • endonuclease activity Source: SGD
  • metal ion binding Source: UniProtKB-KW

GO - Biological processi

  • apoptotic DNA fragmentation Source: SGD
  • cellular response to oxidative stress Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Nuclease

Keywords - Ligandi

Metal-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-28953-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Deoxyribonuclease Tat-D (EC:3.1.21.-)
Gene namesi
Ordered Locus Names:YBL055C
ORF Names:YBL0511, YBL0512
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome II

Organism-specific databases

EuPathDBiFungiDB:YBL055C.
SGDiS000000151. YBL055C.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi185E → A: Reduces enzymatic activities by 50%. 1 Publication1
Mutagenesisi325E → A: Reduces enzymatic activities by 50%. 1 Publication1
Mutagenesisi327D → A: Reduces enzymatic activities by almost 95%. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002019941 – 418Deoxyribonuclease Tat-DAdd BLAST418

Proteomic databases

MaxQBiP34220.
PRIDEiP34220.
TopDownProteomicsiP34220.

Interactioni

Protein-protein interaction databases

BioGridi32644. 26 interactors.
MINTiMINT-2492113.

Structurei

3D structure databases

ProteinModelPortaliP34220.
SMRiP34220.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the TatD-type hydrolase family.Curated

Phylogenomic databases

GeneTreeiENSGT00720000108776.
HOGENOMiHOG000201523.
KOiK03424.
OMAiTHASKAH.
OrthoDBiEOG092C3EIV.

Family and domain databases

InterProiIPR018228. DNase_TatD-rel_CS.
IPR032466. Metal_Hydrolase.
IPR001130. TatD_family.
[Graphical view]
PANTHERiPTHR10060. PTHR10060. 4 hits.
PfamiPF01026. TatD_DNase. 1 hit.
[Graphical view]
SUPFAMiSSF51556. SSF51556. 4 hits.
PROSITEiPS01090. TATD_2. 1 hit.
PS01091. TATD_3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P34220-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MWGILLKSSN KSCSRLWKPI LTQYYSMTST ATDSPLKYYD IGLNLTDPMF
60 70 80 90 100
HGIYNGKQYH PADYVKLLER AAQRHVKNAL VTGSSIAESQ SAIELVSSVK
110 120 130 140 150
DLSPLKLYHT IGVHPCCVNE FADASQGDKA SASIDNPSMD EAYNESLYAK
160 170 180 190 200
VISNPSFAQG KLKELYDLMN QQAKPHDTSF RSIGEIGLDY DRFHYSSKEM
210 220 230 240 250
QKVFFEEQLK ISCLNDKLSS YPLFLHMRSA CDDFVQILER FIAGFTDERD
260 270 280 290 300
TFQLQKLGAS SSSGFYKFHP DRKLVVHSFT GSAIDLQKLL NLSPNIFIGV
310 320 330 340 350
NGCSLRTEEN LAVVKQIPTE RLLLETDAPW CEIKRTHASF QYLAKYQEVR
360 370 380 390 400
DFEYPAFKSV KKNKLADKLN AEELYMVKGR NEPCNMEQVA IVVSEVKDVD
410
LATLIDTTWK TTCKIFGE
Length:418
Mass (Da):47,390
Last modified:February 1, 1994 - v1
Checksum:iF1107C1B8E94CAD1
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti278S → P in AAT92645 (PubMed:17322287).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z23261 Genomic DNA. Translation: CAA80792.1.
Z35816 Genomic DNA. Translation: CAA84875.1.
AY692626 Genomic DNA. Translation: AAT92645.1.
BK006936 Genomic DNA. Translation: DAA07065.1.
PIRiS39833.
RefSeqiNP_009498.1. NM_001178295.1.

Genome annotation databases

EnsemblFungiiYBL055C; YBL055C; YBL055C.
GeneIDi852225.
KEGGisce:YBL055C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z23261 Genomic DNA. Translation: CAA80792.1.
Z35816 Genomic DNA. Translation: CAA84875.1.
AY692626 Genomic DNA. Translation: AAT92645.1.
BK006936 Genomic DNA. Translation: DAA07065.1.
PIRiS39833.
RefSeqiNP_009498.1. NM_001178295.1.

3D structure databases

ProteinModelPortaliP34220.
SMRiP34220.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi32644. 26 interactors.
MINTiMINT-2492113.

Proteomic databases

MaxQBiP34220.
PRIDEiP34220.
TopDownProteomicsiP34220.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYBL055C; YBL055C; YBL055C.
GeneIDi852225.
KEGGisce:YBL055C.

Organism-specific databases

EuPathDBiFungiDB:YBL055C.
SGDiS000000151. YBL055C.

Phylogenomic databases

GeneTreeiENSGT00720000108776.
HOGENOMiHOG000201523.
KOiK03424.
OMAiTHASKAH.
OrthoDBiEOG092C3EIV.

Enzyme and pathway databases

BioCyciYEAST:G3O-28953-MONOMER.

Miscellaneous databases

PROiP34220.

Family and domain databases

InterProiIPR018228. DNase_TatD-rel_CS.
IPR032466. Metal_Hydrolase.
IPR001130. TatD_family.
[Graphical view]
PANTHERiPTHR10060. PTHR10060. 4 hits.
PfamiPF01026. TatD_DNase. 1 hit.
[Graphical view]
SUPFAMiSSF51556. SSF51556. 4 hits.
PROSITEiPS01090. TATD_2. 1 hit.
PS01091. TATD_3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiYBF5_YEAST
AccessioniPrimary (citable) accession number: P34220
Secondary accession number(s): D6VPU5, Q6B2V4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: February 1, 1994
Last modified: November 2, 2016
This is version 118 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 1460 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome II
    Yeast (Saccharomyces cerevisiae) chromosome II: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.