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P34218

- SAS3_YEAST

UniProt

P34218 - SAS3_YEAST

Protein

Histone acetyltransferase SAS3

Gene

SAS3

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 127 (01 Oct 2014)
      Sequence version 1 (01 Feb 1994)
      Previous versions | rss
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    Functioni

    Catalytic component of the histone acetyltransferase NuA3 complex, that acetylates Lys-14 of histone H3. Recruitment of NuA3 to nucleosomes requires methylated histone H3. In conjunction with the FACT complex, NuA3 may be involved in transcriptional regulation. In vitro, SAS3 acetylates free histones H3 and H4. It is involved in silencing the HMR locus.5 Publications

    Catalytic activityi

    Acetyl-CoA + [histone] = CoA + acetyl-[histone].

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei367 – 3671By similarity
    Active sitei418 – 4181NucleophileBy similarity
    Binding sitei421 – 4211Acetyl-CoABy similarity
    Binding sitei456 – 4561Acetyl-CoABy similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri301 – 32323C2HC-typeAdd
    BLAST

    GO - Molecular functioni

    1. histone acetyltransferase activity Source: SGD
    2. metal ion binding Source: UniProtKB-KW
    3. protein binding Source: IntAct

    GO - Biological processi

    1. chromatin modification Source: SGD
    2. chromatin silencing at silent mating-type cassette Source: SGD
    3. chromatin silencing at telomere Source: SGD
    4. histone acetylation Source: SGD
    5. transcription, DNA-templated Source: UniProtKB-KW

    Keywords - Molecular functioni

    Acyltransferase, Chromatin regulator, Transferase

    Keywords - Biological processi

    Transcription, Transcription regulation

    Keywords - Ligandi

    Metal-binding, Zinc

    Enzyme and pathway databases

    BioCyciYEAST:G3O-28951-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Histone acetyltransferase SAS3 (EC:2.3.1.48)
    Alternative name(s):
    Something about silencing protein 3
    Gene namesi
    Name:SAS3
    Ordered Locus Names:YBL052C
    ORF Names:YBL0507, YBL0515
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome II

    Organism-specific databases

    CYGDiYBL052c.
    SGDiS000000148. SAS3.

    Subcellular locationi

    Nucleus 1 Publication

    GO - Cellular componenti

    1. NuA3 histone acetyltransferase complex Source: SGD

    Keywords - Cellular componenti

    Nucleus

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi303 – 3031C → A: Greatly diminishes HAT activity. 2 Publications
    Mutagenesisi306 – 3061C → A: Greatly diminishes HAT activity. 2 Publications
    Mutagenesisi319 – 3191H → A: Greatly diminishes HAT activity. 2 Publications
    Mutagenesisi323 – 3231C → A: Abolishes HAT activity. 2 Publications
    Mutagenesisi426 – 4272QR → AA: Loss of function. 1 Publication
    Mutagenesisi429 – 4313GYG → AAA: Loss of function. 1 Publication
    Mutagenesisi434 – 4352LM → AA: No effect. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 831831Histone acetyltransferase SAS3PRO_0000051579Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei367 – 3671N6-acetyllysine; by autocatalysisBy similarity

    Post-translational modificationi

    Autoacetylation at Lys-367 is required for proper function.By similarity

    Keywords - PTMi

    Acetylation

    Proteomic databases

    MaxQBiP34218.
    PaxDbiP34218.

    Expressioni

    Gene expression databases

    GenevestigatoriP34218.

    Interactioni

    Subunit structurei

    Component of the NuA3 complex, composed of at least NTO1, SAS3, TAF14, YNG1 and EAF6. SAS3 interacts with CDC68/SPT16.2 Publications

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    TAF14P351892EBI-16484,EBI-18920
    YNG1Q084654EBI-16484,EBI-31890

    Protein-protein interaction databases

    BioGridi32646. 93 interactions.
    DIPiDIP-4277N.
    IntActiP34218. 20 interactions.
    MINTiMINT-509916.
    STRINGi4932.YBL052C.

    Structurei

    3D structure databases

    ProteinModelPortaliP34218.
    SMRiP34218. Positions 275-573.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini267 – 573307MYST-type HATAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni426 – 4327Acetyl-CoA bindingBy similarity

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi736 – 83196Asp/Glu-rich (acidic)Add
    BLAST

    Sequence similaritiesi

    Belongs to the MYST (SAS/MOZ) family.Curated
    Contains 1 C2HC-type zinc finger.Curated

    Zinc finger

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri301 – 32323C2HC-typeAdd
    BLAST

    Keywords - Domaini

    Zinc-finger

    Phylogenomic databases

    eggNOGiCOG5027.
    GeneTreeiENSGT00730000114398.
    KOiK11378.
    OrthoDBiEOG7RFTRR.

    Family and domain databases

    Gene3Di3.40.630.30. 1 hit.
    InterProiIPR016181. Acyl_CoA_acyltransferase.
    IPR002717. MOZ_SAS.
    [Graphical view]
    PfamiPF01853. MOZ_SAS. 1 hit.
    [Graphical view]
    SUPFAMiSSF55729. SSF55729. 1 hit.
    PROSITEiPS51726. MYST_HAT. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P34218-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSLTANDESP KPKKNALLKN LEIDDLIHSQ FVRSDTNGHR TTRRLFNSDA    50
    SISHRIRGSV RSDKGLNKIK KGLISQQSKL ASENSSQNIV NRDNKMGAVS 100
    FPIIEPNIEV SEELKVRIKY DSIKFFNFER LISKSSVIAP LVNKNITSSG 150
    PLIGFQRRVN RLKQTWDLAT ENMEYPYSSD NTPFRDNDSW QWYVPYGGTI 200
    KKMKDFSTKR TLPTWEDKIK FLTFLENSKS ATYINGNVSL CNHNETDQEN 250
    EDRKKRKGKV PRIKNKVWFS QIEYIVLRNY EIKPWYTSPF PEHINQNKMV 300
    FICEFCLKYM TSRYTFYRHQ LKCLTFKPPG NEIYRDGKLS VWEIDGRENV 350
    LYCQNLCLLA KCFINSKTLY YDVEPFIFYI LTEREDTENH PYQNAAKFHF 400
    VGYFSKEKFN SNDYNLSCIL TLPIYQRKGY GQFLMEFSYL LSRKESKFGT 450
    PEKPLSDLGL LTYRTFWKIK CAEVLLKLRD SARRRSNNKN EDTFQQVSLN 500
    DIAKLTGMIP TDVVFGLEQL QVLYRHKTRS LSSLDDFNYI IKIDSWNRIE 550
    NIYKTWSSKN YPRVKYDKLL WEPIILGPSF GINGMMNLEP TALADEALTN 600
    ETMAPVISNN THIENYNNSR AHNKRRRRRR RSSEHKTSKL HVNNIIEPEV 650
    PATDFFEDTV SSLTEYMCDY KNTNNDRLIY QAEKRVLESI HDRKGIPRSK 700
    FSTETHWELC FTIKNSETPL GNHAARRNDT GISSLEQDEV ENDVDTELYV 750
    GENAKEDEDE DEDFTLDDDI EDEQISEEND EEEDTYEEDS DDDEDGKRKG 800
    QEQDENDIES HIRKERVRKR RKITLIEDDE E 831
    Length:831
    Mass (Da):97,582
    Last modified:February 1, 1994 - v1
    Checksum:iACF5B1B225CB4A71
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z23261 Genomic DNA. Translation: CAA80794.1.
    Z35814 Genomic DNA. Translation: CAA84873.1.
    BK006936 Genomic DNA. Translation: DAA07067.1.
    PIRiS39835.
    RefSeqiNP_009501.1. NM_001178292.1.

    Genome annotation databases

    EnsemblFungiiYBL052C; YBL052C; YBL052C.
    GeneIDi852228.
    KEGGisce:YBL052C.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z23261 Genomic DNA. Translation: CAA80794.1 .
    Z35814 Genomic DNA. Translation: CAA84873.1 .
    BK006936 Genomic DNA. Translation: DAA07067.1 .
    PIRi S39835.
    RefSeqi NP_009501.1. NM_001178292.1.

    3D structure databases

    ProteinModelPortali P34218.
    SMRi P34218. Positions 275-573.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 32646. 93 interactions.
    DIPi DIP-4277N.
    IntActi P34218. 20 interactions.
    MINTi MINT-509916.
    STRINGi 4932.YBL052C.

    Proteomic databases

    MaxQBi P34218.
    PaxDbi P34218.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YBL052C ; YBL052C ; YBL052C .
    GeneIDi 852228.
    KEGGi sce:YBL052C.

    Organism-specific databases

    CYGDi YBL052c.
    SGDi S000000148. SAS3.

    Phylogenomic databases

    eggNOGi COG5027.
    GeneTreei ENSGT00730000114398.
    KOi K11378.
    OrthoDBi EOG7RFTRR.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-28951-MONOMER.

    Miscellaneous databases

    NextBioi 970757.

    Gene expression databases

    Genevestigatori P34218.

    Family and domain databases

    Gene3Di 3.40.630.30. 1 hit.
    InterProi IPR016181. Acyl_CoA_acyltransferase.
    IPR002717. MOZ_SAS.
    [Graphical view ]
    Pfami PF01853. MOZ_SAS. 1 hit.
    [Graphical view ]
    SUPFAMi SSF55729. SSF55729. 1 hit.
    PROSITEi PS51726. MYST_HAT. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Sequencing and functional analysis of a 32,560 bp segment on the left arm of yeast chromosome II. Identification of 26 open reading frames, including the KIP1 and SEC17 genes."
      Scherens B., el Bakkoury M., Vierendeels F., Dubois E., Messenguy F.
      Yeast 9:1355-1371(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    2. "Complete DNA sequence of yeast chromosome II."
      Feldmann H., Aigle M., Aljinovic G., Andre B., Baclet M.C., Barthe C., Baur A., Becam A.-M., Biteau N., Boles E., Brandt T., Brendel M., Brueckner M., Bussereau F., Christiansen C., Contreras R., Crouzet M., Cziepluch C.
      , Demolis N., Delaveau T., Doignon F., Domdey H., Duesterhus S., Dubois E., Dujon B., El Bakkoury M., Entian K.-D., Feuermann M., Fiers W., Fobo G.M., Fritz C., Gassenhuber J., Glansdorff N., Goffeau A., Grivell L.A., de Haan M., Hein C., Herbert C.J., Hollenberg C.P., Holmstroem K., Jacq C., Jacquet M., Jauniaux J.-C., Jonniaux J.-L., Kallesoee T., Kiesau P., Kirchrath L., Koetter P., Korol S., Liebl S., Logghe M., Lohan A.J.E., Louis E.J., Li Z.Y., Maat M.J., Mallet L., Mannhaupt G., Messenguy F., Miosga T., Molemans F., Mueller S., Nasr F., Obermaier B., Perea J., Pierard A., Piravandi E., Pohl F.M., Pohl T.M., Potier S., Proft M., Purnelle B., Ramezani Rad M., Rieger M., Rose M., Schaaff-Gerstenschlaeger I., Scherens B., Schwarzlose C., Skala J., Slonimski P.P., Smits P.H.M., Souciet J.-L., Steensma H.Y., Stucka R., Urrestarazu L.A., van der Aart Q.J.M., Van Dyck L., Vassarotti A., Vetter I., Vierendeels F., Vissers S., Wagner G., de Wergifosse P., Wolfe K.H., Zagulski M., Zimmermann F.K., Mewes H.-W., Kleine K.
      EMBO J. 13:5795-5809(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    3. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    4. "The something about silencing protein, Sas3, is the catalytic subunit of NuA3, a yTAF(II)30-containing HAT complex that interacts with the Spt16 subunit of the yeast CP (Cdc68/Pob3)-FACT complex."
      John S., Howe L., Tafrov S.T., Grant P.A., Sternglanz R., Workman J.L.
      Genes Dev. 14:1196-1208(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 799-813, FUNCTION, INTERACTION WITH SPT16, MUTAGENESIS OF 426-GLN-ARG-427; 429-GLY--GLY-431 AND 434-LEU-MET-435.
    5. "Yeast SAS silencing genes and human genes associated with AML and HIV-1 Tat interactions are homologous with acetyltransferases."
      Reifsnyder C., Lowell J., Clarke A., Pillus L.
      Nat. Genet. 14:42-49(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: CHARACTERIZATION.
    6. Erratum
      Reifsnyder C., Lowell J., Clarke A., Pillus L.
      Nat. Genet. 16:109-109(1997) [PubMed] [Europe PMC] [Abstract]
    7. "Sas3 is a histone acetyltransferase and requires a zinc finger motif."
      Takechi S., Nakayama T.
      Biochem. Biophys. Res. Commun. 266:405-410(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, MUTAGENESIS OF CYS-303; CYS-306; HIS-319 AND CYS-323.
    8. "Histone H3 specific acetyltransferases are essential for cell cycle progression."
      Howe L., Auston D., Grant P., John S., Cook R.G., Workman J.L., Pillus L.
      Genes Dev. 15:3144-3154(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    9. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
    10. "Methylation of histone H3 mediates the association of the NuA3 histone acetyltransferase with chromatin."
      Martin D.G., Grimes D.E., Baetz K., Howe L.
      Mol. Cell. Biol. 26:3018-3028(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION OF THE NUA3 COMPLEX.
    11. "Yng1 PHD finger binding to H3 trimethylated at K4 promotes NuA3 HAT activity at K14 of H3 and transcription at a subset of targeted ORFs."
      Taverna S.D., Ilin S., Rogers R.S., Tanny J.C., Lavender H., Li H., Baker L., Boyle J., Blair L.P., Chait B.T., Patel D.J., Aitchison J.D., Tackett A.J., Allis C.D.
      Mol. Cell 24:785-796(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION OF THE NUA3 COMPLEX, IDENTIFICATION IN THE NUA3 COMPLEX, IDENTIFICATION BY MASS SPECTROMETRY.

    Entry informationi

    Entry nameiSAS3_YEAST
    AccessioniPrimary (citable) accession number: P34218
    Secondary accession number(s): D6VPU7
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1994
    Last sequence update: February 1, 1994
    Last modified: October 1, 2014
    This is version 127 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    3. Yeast chromosome II
      Yeast (Saccharomyces cerevisiae) chromosome II: entries and gene names

    External Data

    Dasty 3