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Protein

Histone acetyltransferase SAS3

Gene

SAS3

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic component of the histone acetyltransferase NuA3 complex, that acetylates Lys-14 of histone H3. Recruitment of NuA3 to nucleosomes requires methylated histone H3. In conjunction with the FACT complex, NuA3 may be involved in transcriptional regulation. In vitro, SAS3 acetylates free histones H3 and H4. It is involved in silencing the HMR locus.5 Publications

Catalytic activityi

Acetyl-CoA + [histone] = CoA + acetyl-[histone].2 Publications

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei452 – 4521Proton donor/acceptorBy similarity
Binding sitei456 – 4561Acetyl-CoABy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri300 – 32526C2HC MYST-typePROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

  • histone acetyltransferase activity Source: SGD
  • metal ion binding Source: UniProtKB-KW

GO - Biological processi

  • chromatin modification Source: SGD
  • chromatin silencing at silent mating-type cassette Source: SGD
  • chromatin silencing at telomere Source: SGD
  • histone acetylation Source: SGD
  • transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Acyltransferase, Chromatin regulator, Transferase

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

BioCyciYEAST:G3O-28951-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Histone acetyltransferase SAS3 (EC:2.3.1.482 Publications)
Alternative name(s):
Something about silencing protein 31 Publication
Gene namesi
Name:SAS3
Ordered Locus Names:YBL052C
ORF Names:YBL0507, YBL0515
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome II

Organism-specific databases

EuPathDBiFungiDB:YBL052C.
SGDiS000000148. SAS3.

Subcellular locationi

GO - Cellular componenti

  • chromosome, telomeric region Source: GOC
  • NuA3a histone acetyltransferase complex Source: SGD
  • NuA3b histone acetyltransferase complex Source: SGD
  • NuA3 histone acetyltransferase complex Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi303 – 3031C → A: Greatly diminishes HAT activity. 2 Publications
Mutagenesisi306 – 3061C → A: Greatly diminishes HAT activity. 2 Publications
Mutagenesisi319 – 3191H → A: Greatly diminishes HAT activity. 2 Publications
Mutagenesisi323 – 3231C → A: Abolishes HAT activity. 2 Publications
Mutagenesisi426 – 4272QR → AA: Loss of function. 1 Publication
Mutagenesisi429 – 4313GYG → AAA: Loss of function. 1 Publication
Mutagenesisi434 – 4352LM → AA: No effect. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 831831Histone acetyltransferase SAS3PRO_0000051579Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei367 – 3671N6-acetyllysine; by autocatalysisBy similarity

Post-translational modificationi

Autoacetylation at Lys-367 is required for proper function.By similarity

Keywords - PTMi

Acetylation

Proteomic databases

MaxQBiP34218.

Interactioni

Subunit structurei

Component of the NuA3 complex, composed of at least NTO1, SAS3, TAF14, YNG1 and EAF6. SAS3 interacts with CDC68/SPT16.2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
TAF14P351893EBI-16484,EBI-18920
YNG1Q084655EBI-16484,EBI-31890

Protein-protein interaction databases

BioGridi32646. 94 interactions.
DIPiDIP-4277N.
IntActiP34218. 25 interactions.
MINTiMINT-509916.

Structurei

3D structure databases

ProteinModelPortaliP34218.
SMRiP34218. Positions 275-573.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini267 – 573307MYST-type HATPROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni419 – 4213Acetyl-CoA bindingBy similarity
Regioni426 – 4327Acetyl-CoA bindingBy similarity

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi736 – 83196Asp/Glu-rich (acidic)Add
BLAST

Sequence similaritiesi

Belongs to the MYST (SAS/MOZ) family.Curated
Contains 1 C2HC MYST-type zinc finger.PROSITE-ProRule annotation
Contains 1 MYST-type HAT (histone acetyltransferase) domain.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri300 – 32526C2HC MYST-typePROSITE-ProRule annotationAdd
BLAST

Keywords - Domaini

Zinc-finger

Phylogenomic databases

GeneTreeiENSGT00730000114398.
InParanoidiP34218.
KOiK11378.
OrthoDBiEOG092C043Q.

Family and domain databases

Gene3Di3.40.630.30. 1 hit.
InterProiIPR016181. Acyl_CoA_acyltransferase.
IPR002717. HAT_MYST-type.
[Graphical view]
PfamiPF01853. MOZ_SAS. 1 hit.
[Graphical view]
SUPFAMiSSF55729. SSF55729. 1 hit.
PROSITEiPS51726. MYST_HAT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P34218-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSLTANDESP KPKKNALLKN LEIDDLIHSQ FVRSDTNGHR TTRRLFNSDA
60 70 80 90 100
SISHRIRGSV RSDKGLNKIK KGLISQQSKL ASENSSQNIV NRDNKMGAVS
110 120 130 140 150
FPIIEPNIEV SEELKVRIKY DSIKFFNFER LISKSSVIAP LVNKNITSSG
160 170 180 190 200
PLIGFQRRVN RLKQTWDLAT ENMEYPYSSD NTPFRDNDSW QWYVPYGGTI
210 220 230 240 250
KKMKDFSTKR TLPTWEDKIK FLTFLENSKS ATYINGNVSL CNHNETDQEN
260 270 280 290 300
EDRKKRKGKV PRIKNKVWFS QIEYIVLRNY EIKPWYTSPF PEHINQNKMV
310 320 330 340 350
FICEFCLKYM TSRYTFYRHQ LKCLTFKPPG NEIYRDGKLS VWEIDGRENV
360 370 380 390 400
LYCQNLCLLA KCFINSKTLY YDVEPFIFYI LTEREDTENH PYQNAAKFHF
410 420 430 440 450
VGYFSKEKFN SNDYNLSCIL TLPIYQRKGY GQFLMEFSYL LSRKESKFGT
460 470 480 490 500
PEKPLSDLGL LTYRTFWKIK CAEVLLKLRD SARRRSNNKN EDTFQQVSLN
510 520 530 540 550
DIAKLTGMIP TDVVFGLEQL QVLYRHKTRS LSSLDDFNYI IKIDSWNRIE
560 570 580 590 600
NIYKTWSSKN YPRVKYDKLL WEPIILGPSF GINGMMNLEP TALADEALTN
610 620 630 640 650
ETMAPVISNN THIENYNNSR AHNKRRRRRR RSSEHKTSKL HVNNIIEPEV
660 670 680 690 700
PATDFFEDTV SSLTEYMCDY KNTNNDRLIY QAEKRVLESI HDRKGIPRSK
710 720 730 740 750
FSTETHWELC FTIKNSETPL GNHAARRNDT GISSLEQDEV ENDVDTELYV
760 770 780 790 800
GENAKEDEDE DEDFTLDDDI EDEQISEEND EEEDTYEEDS DDDEDGKRKG
810 820 830
QEQDENDIES HIRKERVRKR RKITLIEDDE E
Length:831
Mass (Da):97,582
Last modified:February 1, 1994 - v1
Checksum:iACF5B1B225CB4A71
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z23261 Genomic DNA. Translation: CAA80794.1.
Z35814 Genomic DNA. Translation: CAA84873.1.
BK006936 Genomic DNA. Translation: DAA07067.1.
PIRiS39835.
RefSeqiNP_009501.1. NM_001178292.1.

Genome annotation databases

EnsemblFungiiYBL052C; YBL052C; YBL052C.
GeneIDi852228.
KEGGisce:YBL052C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z23261 Genomic DNA. Translation: CAA80794.1.
Z35814 Genomic DNA. Translation: CAA84873.1.
BK006936 Genomic DNA. Translation: DAA07067.1.
PIRiS39835.
RefSeqiNP_009501.1. NM_001178292.1.

3D structure databases

ProteinModelPortaliP34218.
SMRiP34218. Positions 275-573.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi32646. 94 interactions.
DIPiDIP-4277N.
IntActiP34218. 25 interactions.
MINTiMINT-509916.

Proteomic databases

MaxQBiP34218.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYBL052C; YBL052C; YBL052C.
GeneIDi852228.
KEGGisce:YBL052C.

Organism-specific databases

EuPathDBiFungiDB:YBL052C.
SGDiS000000148. SAS3.

Phylogenomic databases

GeneTreeiENSGT00730000114398.
InParanoidiP34218.
KOiK11378.
OrthoDBiEOG092C043Q.

Enzyme and pathway databases

BioCyciYEAST:G3O-28951-MONOMER.

Miscellaneous databases

PROiP34218.

Family and domain databases

Gene3Di3.40.630.30. 1 hit.
InterProiIPR016181. Acyl_CoA_acyltransferase.
IPR002717. HAT_MYST-type.
[Graphical view]
PfamiPF01853. MOZ_SAS. 1 hit.
[Graphical view]
SUPFAMiSSF55729. SSF55729. 1 hit.
PROSITEiPS51726. MYST_HAT. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSAS3_YEAST
AccessioniPrimary (citable) accession number: P34218
Secondary accession number(s): D6VPU7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: February 1, 1994
Last modified: September 7, 2016
This is version 141 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome II
    Yeast (Saccharomyces cerevisiae) chromosome II: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.