RNA-binding protein PIN4 - Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

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P34217 (PIN4_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 110. History...

Clusters with 100%, 90%, 50% identity |

Documents (4) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
RNA-binding protein PIN4

Alternative name(s):
Psi inducibility protein 4

Gene names

Name:	PIN4
Synonyms:	MDT1
Ordered Locus Names:	YBL051C
ORF Names:	YBL0506, YBL0516

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces ›

Protein attributes

Sequence length	668 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Involved in normal G2/M phase transition of the mitotic cell cycle. In association with RAD53, also involved in checkpoint control in response to DNA damage. Ref.6
Subunit structure	Interacts with RAD53. Ref.6
Subcellular location	Cytoplasm Ref.4.
Post-translational modification	Hyperphosphorylated in response to DNA damage by MEC1. Ref.6
Miscellaneous	Present with 4630 molecules/cell in log phase SD medium.
Sequence similarities	Contains 1 RRM (RNA recognition motif) domain.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	RNA-binding
PTM	Phosphoprotein
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	DNA damage checkpoint Inferred from physical interaction Ref.6. Source: SGD G2/M transition of mitotic cell cycle Inferred from mutant phenotype Ref.6. Source: SGD
Cellular_component	cytoplasm Inferred from direct assay Ref.4. Source: SGD
Molecular_function	RNA binding Inferred from electronic annotation. Source: UniProtKB-KW nucleotide binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
RAD53	P22216	3	EBI-21256,EBI-17843

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 668

668

RNA-binding protein PIN4

PRO_0000082028

Regions

Domain

85 – 163

RRM

Amino acid modifications

Modified residue

Phosphoserine Ref.11

Modified residue

Phosphoserine Ref.11

Modified residue

189

Phosphoserine Ref.8 Ref.9 Ref.10

Modified residue

190

Phosphoserine Ref.9 Ref.11

Modified residue

191

Phosphoserine Ref.11

Modified residue

194

Phosphoserine Ref.10 Ref.11

Modified residue

197

Phosphoserine Ref.11

Modified residue

199

Phosphoserine Ref.11

Modified residue

262

Phosphoserine Ref.11

Modified residue

305

Phosphothreonine; by ATR

Modified residue

397

Phosphoserine Ref.11

Modified residue

399

Phosphoserine Ref.11

Modified residue

400

Phosphotyrosine Ref.11

Modified residue

402

Phosphoserine Ref.11

Modified residue

454

Phosphoserine Ref.11

Modified residue

466

Phosphoserine Ref.7 Ref.8 Ref.11

Modified residue

526

Phosphotyrosine Ref.11

Modified residue

541

Phosphoserine Ref.7

Modified residue

579

Phosphoserine Ref.11

Modified residue

629

Phosphoserine Ref.11

Modified residue

636

Phosphoserine Ref.10 Ref.11

Modified residue

638

Phosphoserine Ref.7 Ref.10 Ref.11

Modified residue

640

Phosphoserine Ref.10 Ref.11

Modified residue

653

Phosphoserine Ref.10 Ref.11

Modified residue

655

Phosphoserine Ref.7 Ref.10 Ref.11

Experimental info

Mutagenesis

305

T → A: No interaction with RAD53. Ref.6

Sequences

Sequence

Length

Mass (Da)

Tools

P34217 [UniParc].

Last modified February 1, 1994. Version 1.
Checksum: EA8EE581286436CB
Show »

FASTA

668

73,776

        10         20         30         40         50         60 
METSSFENAP PAAINDAQDN NINTETNDQE TNQQSIETRD AIDKENGVQT ETGENSAKNA 

        70         80         90        100        110        120 
EQNVSSTNLN NAPTNGALDD DVIPNAIVIK NIPFAIKKEQ LLDIIEEMDL PLPYAFNYHF 

       130        140        150        160        170        180 
DNGIFRGLAF ANFTTPEETT QVITSLNGKE ISGRKLKVEY KKMLPQAERE RIEREKREKR 

       190        200        210        220        230        240 
GQLEEQHRSS SNLSLDSLSK MSGSGNNNTS NNQLFSTLMN GINANSMMNS PMNNTINNNS 

       250        260        270        280        290        300 
SNNNNSGNII LNQPSLSAQH TSSSLYQTNV NNQAQMSTER FYAPLPSTST LPLPPQQLDF 

       310        320        330        340        350        360 
NDPDTLEIYS QLLLFKDREK YYYELAYPMG ISASHKRIIN VLCSYLGLVE VYDPRFIIIR 

       370        380        390        400        410        420 
RKILDHANLQ SHLQQQGQMT SAHPLQPNST GGSMNRSQSY TSLLQAHAAA AANSISNQAV 

       430        440        450        460        470        480 
NNSSNSNTIN SNNGNGNNVI INNNSASSTP KISSQGQFSM QPTLTSPKMN IHHSSQYNSA 

       490        500        510        520        530        540 
DQPQQPQPQT QQNVQSAAQQ QQSFLRQQAT LTPSSRIPSG YSANHYQINS VNPLLRNSQI 

       550        560        570        580        590        600 
SPPNSQIPIN SQTLSQAQPP AQSQTQQRVP VAYQNASLSS QQLYNLNGPS SANSQSQLLP 

       610        620        630        640        650        660 
QHTNGSVHSN FSYQSYHDES MLSAHNLNSA DLIYKSLSHS GLDDGLEQGL NRSLSGLDLQ 


NQNKKNLW

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequencing and functional analysis of a 32,560 bp segment on the left arm of yeast chromosome II. Identification of 26 open reading frames, including the KIP1 and SEC17 genes." Scherens B., el Bakkoury M., Vierendeels F., Dubois E., Messenguy F. Yeast 9:1355-1371(1993) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[2]	"Complete DNA sequence of yeast chromosome II." Feldmann H., Aigle M., Aljinovic G., Andre B., Baclet M.C., Barthe C., Baur A., Becam A.-M., Biteau N., Boles E., Brandt T., Brendel M., Brueckner M., Bussereau F., Christiansen C., Contreras R., Crouzet M., Cziepluch C. Kleine K. EMBO J. 13:5795-5809(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[3]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[4]	"Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[5]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[6]	"Mdt1, a novel Rad53 FHA1 domain-interacting protein, modulates DNA damage tolerance and G(2)/M cell cycle progression in Saccharomyces cerevisiae." Pike B.L., Yongkiettrakul S., Tsai M.-D., Heierhorst J. Mol. Cell. Biol. 24:2779-2788(2004) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, INTERACTION WITH RAD53, PHOSPHORYLATION, MUTAGENESIS OF THR-305.
[7]	"Quantitative phosphoproteomics applied to the yeast pheromone signaling pathway." Gruhler A., Olsen J.V., Mohammed S., Mortensen P., Faergeman N.J., Mann M., Jensen O.N. Mol. Cell. Proteomics 4:310-327(2005) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-466; SER-541; SER-638 AND SER-655, MASS SPECTROMETRY. Strain: YAL6B.
[8]	"Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae." Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P. J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-189 AND SER-466, MASS SPECTROMETRY. Strain: ADR376.
[9]	"Analysis of phosphorylation sites on proteins from Saccharomyces cerevisiae by electron transfer dissociation (ETD) mass spectrometry." Chi A., Huttenhower C., Geer L.Y., Coon J.J., Syka J.E.P., Bai D.L., Shabanowitz J., Burke D.J., Troyanskaya O.G., Hunt D.F. Proc. Natl. Acad. Sci. U.S.A. 104:2193-2198(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-189 AND SER-190, MASS SPECTROMETRY.
[10]	"Proteome-wide identification of in vivo targets of DNA damage checkpoint kinases." Smolka M.B., Albuquerque C.P., Chen S.H., Zhou H. Proc. Natl. Acad. Sci. U.S.A. 104:10364-10369(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-189; SER-194; SER-636; SER-638; SER-640; SER-653 AND SER-655, MASS SPECTROMETRY.
[11]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-56; SER-65; SER-190; SER-191; SER-194; SER-197; SER-199; SER-262; SER-397; SER-399; TYR-400; SER-402; SER-454; SER-466; TYR-526; SER-579; SER-629; SER-636; SER-638; SER-640; SER-653 AND SER-655, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

Z23261 Genomic DNA. Translation: CAA80795.1.
Z35812 Genomic DNA. Translation: CAA84871.1.
BK006936 Genomic DNA. Translation: DAA07068.1.

PIR

S39836.

RefSeq

NP_009502.1. NM_001178291.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2A0T	NMR	-	B	301-310	[»]

ProteinModelPortal

P34217.

ModBase

Search...

Protein-protein interaction databases

DIP

DIP-1905N.

IntAct

P34217. 42 interactions.

MINT

MINT-388641.

STRING

4932.YBL051C.

Proteomic databases

PaxDb

P34217.

PeptideAtlas

P34217.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblFungi

YBL051C; YBL051C; YBL051C.

GeneID

852229.

KEGG

sce:YBL051C.

Organism-specific databases

CYGD

YBL051c.

SGD

S000000147. PIN4.

Phylogenomic databases

eggNOG

COG0724.

HOGENOM

HOG000115549.

OMA

KKMLPQA.

OrthoDB

EOG4QG0PB.

Gene expression databases

Genevestigator

P34217.

GermOnline

YBL051C. Saccharomyces cerevisiae.

Family and domain databases

Gene3D

3.30.70.330. 1 hit.

InterPro

IPR012677. Nucleotide-bd_a/b_plait.
IPR000504. RRM_dom.
[Graphical view]

SMART

SM00360. RRM. 1 hit.
[Graphical view]

PROSITE

PS50102. RRM. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

EvolutionaryTrace

P34217.

NextBio

970760.

Entry information

Entry name

PIN4_YEAST

Accession

Primary (citable) accession number: P34217
Secondary accession number(s): D6VPU8

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1994
Last sequence update:	February 1, 1994
Last modified:	May 1, 2013
This is version 110 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program

Relevant documents

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

Yeast chromosome II

Yeast (Saccharomyces cerevisiae) chromosome II: entries and gene names

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Binary interactions

With

Entry

#Exp.

IntAct

Notes

Sequence annotation (Features)

Molecule processing

Regions

Amino acid modifications

Experimental info

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents