RNA-binding protein PIN4 - Saccharomyces cerevisiae (Baker's yeast)

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Reviewed, UniProtKB/Swiss-Prot P34217 (PIN4_YEAST)

Last modified November 3, 2009. Version 82. History...

Clusters with 100%, 90%, 50% identity |

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Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Binary interactions · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein names

Recommended name:
RNA-binding protein PIN4
Alternative name(s):
Psi inducibility protein 4

Gene names

Name:	PIN4
Synonyms:	MDT1
Ordered Locus Names:	YBL051C
ORF Names:	YBL0516, YBL0506

Organism

Saccharomyces cerevisiae (Baker's yeast) [Complete proteome]

Taxonomic identifier

4932 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

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Protein attributes

Sequence length	668 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

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General annotation (Comments)

Function	Involved in normal G2/M phase transition of the mitotic cell cycle. In association with RAD53, also involved in checkpoint control in response to DNA damage. Ref.5
Subunit structure	Interacts with RAD53. Ref.5
Subcellular location	Cytoplasm. Ref.3
Post-translational modification	Hyperphosphorylated in response to DNA damage by MEC1. Ref.5 Ref.6 Ref.7 Ref.8 Ref.9 Ref.10
Miscellaneous	Present with 4630 molecules/cell in log phase SD medium. Ref.4
Sequence similarities	Contains 1 RRM (RNA recognition motif) domain.

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Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	RNA-binding
PTM	Phosphoprotein
Technical term	3D-structure Complete proteome
Gene Ontology (GO)
Biological process	DNA damage checkpoint Ref.5 Inferred from genetic interaction. Source: SGD G2/M transition of mitotic cell cycle Ref.5 Inferred from mutant phenotype. Source: SGD
Cellular component	cytoplasm Ref.3 Inferred from direct assay. Source: SGD
Molecular function	RNA binding Inferred from electronic annotation. Source: UniProtKB-KW nucleotide binding Inferred from electronic annotation. Source: InterPro protein binding Ref.5 Inferred from physical interaction. Source: IntAct
Complete GO annotation...

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Binary interactions

With	Entry	#Exp.	IntAct
CAF40	P53829	1	EBI-21256,EBI-28306
MEC1	P38111	1	EBI-21256,EBI-6668
NOT1	P25655	1	EBI-21256,EBI-12139
NOT2	P06100	1	EBI-21256,EBI-12153
NOT4	P34909	1	EBI-21256,EBI-12174
RAD53	P22216	3	EBI-21256,EBI-17843

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Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 668

668

RNA-binding protein PIN4

PRO_0000082028

Regions

Domain

85 – 163

RRM

Amino acid modifications

Modified residue

Phosphoserine Ref.10

Modified residue

Phosphoserine Ref.10

Modified residue

189

Phosphoserine Ref.7 Ref.8 Ref.9

Modified residue

190

Phosphoserine Ref.8 Ref.10

Modified residue

191

Phosphoserine Ref.10

Modified residue

194

Phosphoserine Ref.9 Ref.10

Modified residue

197

Phosphoserine Ref.10

Modified residue

199

Phosphoserine Ref.10

Modified residue

262

Phosphoserine Ref.10

Modified residue

305

Phosphothreonine; by ATR

Modified residue

397

Phosphoserine Ref.10

Modified residue

399

Phosphoserine Ref.10

Modified residue

400

Phosphotyrosine Ref.10

Modified residue

402

Phosphoserine Ref.10

Modified residue

454

Phosphoserine Ref.10

Modified residue

466

Phosphoserine Ref.6 Ref.7 Ref.10

Modified residue

526

Phosphotyrosine Ref.10

Modified residue

541

Phosphoserine Ref.6

Modified residue

579

Phosphoserine Ref.10

Modified residue

629

Phosphoserine Ref.10

Modified residue

636

Phosphoserine Ref.9 Ref.10

Modified residue

638

Phosphoserine Ref.6 Ref.9 Ref.10

Modified residue

640

Phosphoserine Ref.9 Ref.10

Modified residue

653

Phosphoserine Ref.9 Ref.10

Modified residue

655

Phosphoserine Ref.6 Ref.9 Ref.10

Experimental info

Mutagenesis

305

T → A: No interaction with RAD53. Ref.5

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Sequences

Sequence

Length

Mass (Da)

Tools

P34217-1 [UniParc].

Last modified February 1, 1994. Version 1.
Checksum: EA8EE581286436CB
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FASTA

668

73,776

        10         20         30         40         50         60 
METSSFENAP PAAINDAQDN NINTETNDQE TNQQSIETRD AIDKENGVQT ETGENSAKNA 

        70         80         90        100        110        120 
EQNVSSTNLN NAPTNGALDD DVIPNAIVIK NIPFAIKKEQ LLDIIEEMDL PLPYAFNYHF 

       130        140        150        160        170        180 
DNGIFRGLAF ANFTTPEETT QVITSLNGKE ISGRKLKVEY KKMLPQAERE RIEREKREKR 

       190        200        210        220        230        240 
GQLEEQHRSS SNLSLDSLSK MSGSGNNNTS NNQLFSTLMN GINANSMMNS PMNNTINNNS 

       250        260        270        280        290        300 
SNNNNSGNII LNQPSLSAQH TSSSLYQTNV NNQAQMSTER FYAPLPSTST LPLPPQQLDF 

       310        320        330        340        350        360 
NDPDTLEIYS QLLLFKDREK YYYELAYPMG ISASHKRIIN VLCSYLGLVE VYDPRFIIIR 

       370        380        390        400        410        420 
RKILDHANLQ SHLQQQGQMT SAHPLQPNST GGSMNRSQSY TSLLQAHAAA AANSISNQAV 

       430        440        450        460        470        480 
NNSSNSNTIN SNNGNGNNVI INNNSASSTP KISSQGQFSM QPTLTSPKMN IHHSSQYNSA 

       490        500        510        520        530        540 
DQPQQPQPQT QQNVQSAAQQ QQSFLRQQAT LTPSSRIPSG YSANHYQINS VNPLLRNSQI 

       550        560        570        580        590        600 
SPPNSQIPIN SQTLSQAQPP AQSQTQQRVP VAYQNASLSS QQLYNLNGPS SANSQSQLLP 

       610        620        630        640        650        660 
QHTNGSVHSN FSYQSYHDES MLSAHNLNSA DLIYKSLSHS GLDDGLEQGL NRSLSGLDLQ 


NQNKKNLW

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References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequencing and functional analysis of a 32,560 bp segment on the left arm of yeast chromosome II. Identification of 26 open reading frames, including the KIP1 and SEC17 genes." Scherens B., el Bakkoury M., Vierendeels F., Dubois E., Messenguy F. Yeast 9:1355-1371(1993) [PubMed: 8154187] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[2]	"Complete DNA sequence of yeast chromosome II." Feldmann H., Aigle M., Aljinovic G., Andre B., Baclet M.C., Barthe C., Baur A., Becam A.-M., Biteau N., Boles E., Brandt T., Brendel M., Brueckner M., Bussereau F., Christiansen C., Contreras R., Crouzet M., Cziepluch C. Kleine K. EMBO J. 13:5795-5809(1994) [PubMed: 7813418] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[3]	"Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed: 14562095] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[4]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[5]	"Mdt1, a novel Rad53 FHA1 domain-interacting protein, modulates DNA damage tolerance and G(2)/M cell cycle progression in Saccharomyces cerevisiae." Pike B.L., Yongkiettrakul S., Tsai M.-D., Heierhorst J. Mol. Cell. Biol. 24:2779-2788(2004) [PubMed: 15024067] [Abstract] Cited for: FUNCTION, INTERACTION WITH RAD53, PHOSPHORYLATION, MUTAGENESIS OF THR-305.
[6]	"Quantitative phosphoproteomics applied to the yeast pheromone signaling pathway." Gruhler A., Olsen J.V., Mohammed S., Mortensen P., Faergeman N.J., Mann M., Jensen O.N. Mol. Cell. Proteomics 4:310-327(2005) [PubMed: 15665377] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-466; SER-541; SER-638 AND SER-655, MASS SPECTROMETRY.
[7]	"Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae." Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P. J. Proteome Res. 6:1190-1197(2007) [PubMed: 17330950] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-189 AND SER-466, MASS SPECTROMETRY.
[8]	"Analysis of phosphorylation sites on proteins from Saccharomyces cerevisiae by electron transfer dissociation (ETD) mass spectrometry." Chi A., Huttenhower C., Geer L.Y., Coon J.J., Syka J.E.P., Bai D.L., Shabanowitz J., Burke D.J., Troyanskaya O.G., Hunt D.F. Proc. Natl. Acad. Sci. U.S.A. 104:2193-2198(2007) [PubMed: 17287358] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-189 AND SER-190, MASS SPECTROMETRY.
[9]	"Proteome-wide identification of in vivo targets of DNA damage checkpoint kinases." Smolka M.B., Albuquerque C.P., Chen S.H., Zhou H. Proc. Natl. Acad. Sci. U.S.A. 104:10364-10369(2007) [PubMed: 17563356] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-189; SER-194; SER-636; SER-638; SER-640; SER-653 AND SER-655, MASS SPECTROMETRY.
[10]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed: 18407956] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-56; SER-65; SER-190; SER-191; SER-194; SER-197; SER-199; SER-262; SER-397; SER-399; TYR-400; SER-402; SER-454; SER-466; TYR-526; SER-579; SER-629; SER-636; SER-638; SER-640; SER-653 AND SER-655, MASS SPECTROMETRY.
+	Additional computationally mapped references.

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Cross-references

Sequence databases

Z23261 Genomic DNA. Translation: CAA80795.1.
Z35812 Genomic DNA. Translation: CAA84871.1.

PIR

S39836.

RefSeq

NP_009502.1.

3D structure databases

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2A0T	NMR	-	B	301-310	[»]

ModBase

Search...

Protein-protein interaction databases

DIP

DIP:1905N.

IntAct

P34217. 46 interactions.

STRING

P34217.

Proteomic databases

PeptideAtlas

P34217.

Genome annotation databases

Ensembl

YBL051C; YBL051C; YBL051C; Saccharomyces cerevisiae. [Genome view]

GeneID

852229.

GenomeReviews

Gene locus YBL051C in contig Y13134_GR.

KEGG

sce:YBL051C.

NMPDR

fig|4932.3.peg.192.

Organism-specific databases

CYGD

YBL051c.

SGD

S000000147. PIN4.

Phylogenomic databases

HOGENOM

P34217.

OMA

MLPQAER.

Gene expression databases

ArrayExpress

P34217.

Genevestigator

P34217.

GermOnline

YBL051C. Saccharomyces cerevisiae.

Family and domain databases

InterPro

IPR012677. a_b_plait_nuc_bd.
IPR000504. RRM_RNP1.
[Graphical view]

Gene3D

G3DSA:3.30.70.330. a_b_plait_nuc_bd. 1 hit.

Pfam

PF00076. RRM_1. 1 hit.
[Graphical view]

SMART

SM00360. RRM. 1 hit.
[Graphical view]

PROSITE

PS50102. RRM. 1 hit.
[Graphical view]

ProtoNet

Search...

Other Resources

NextBio

970760.

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Entry information

Entry name

PIN4_YEAST

Accession

Primary (citable) accession number: P34217

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1994
Last sequence update:	February 1, 1994
Last modified:	November 3, 2009
This is version 82 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)

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Relevant documents

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

Yeast chromosome II

Yeast (Saccharomyces cerevisiae) chromosome II: entries and gene names

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Binary interactions

With

Entry

#Exp.

IntAct

Notes

Sequence annotation (Features)

Molecule processing

Regions

Amino acid modifications

Experimental info

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Proteomic databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Other Resources

Entry information

Relevant documents