P34217 (PIN4_YEAST) Reviewed, UniProtKB/Swiss-Prot
Last modified
May 1, 2013.
Version 110.
History...
Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order
Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize orderNames and origin
| Protein names | Recommended name: RNA-binding protein PIN4 Alternative name(s): Psi inducibility protein 4 | ||||||||
| Gene names |
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| Organism | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome] | ||||||||
| Taxonomic identifier | 559292 [NCBI] | ||||||||
| Taxonomic lineage | Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › ![]() |
Protein attributes
| Sequence length | 668 AA. |
| Sequence status | Complete. |
| Protein existence | Evidence at protein level |
General annotation (Comments)
| Function | Involved in normal G2/M phase transition of the mitotic cell cycle. In association with RAD53, also involved in checkpoint control in response to DNA damage. Ref.6 |
| Subunit structure | Interacts with RAD53. Ref.6 |
| Subcellular location | |
| Post-translational modification | Hyperphosphorylated in response to DNA damage by MEC1. Ref.6 |
| Miscellaneous | Present with 4630 molecules/cell in log phase SD medium. |
| Sequence similarities | Contains 1 RRM (RNA recognition motif) domain. |
Ontologies
| Keywords | |
|---|---|
| Cellular component | Cytoplasm |
| Ligand | RNA-binding |
| PTM | Phosphoprotein |
| Technical term | 3D-structure Complete proteome Reference proteome |
| Gene Ontology (GO) | |
| Biological_process | DNA damage checkpoint Inferred from physical interaction Ref.6. Source: SGD G2/M transition of mitotic cell cycleInferred from mutant phenotype Ref.6. Source: SGD |
| Cellular_component | cytoplasm Inferred from direct assay Ref.4. Source: SGD |
| Molecular_function | RNA binding Inferred from electronic annotation. Source: UniProtKB-KW nucleotide bindingInferred from electronic annotation. Source: InterPro |
| Complete GO annotation... | |
Binary interactions
With | Entry | #Exp. | IntAct | Notes |
|---|---|---|---|---|
| RAD53 | P22216 | 3 | EBI-21256,EBI-17843 |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Chain | 1 – 668 | 668 | RNA-binding protein PIN4 | PRO_0000082028 | |||||
Regions | |||||||||
| Domain | 85 – 163 | 79 | RRM | ||||||
Amino acid modifications | |||||||||
| Modified residue | 56 | 1 | Phosphoserine Ref.11 | ||||||
| Modified residue | 65 | 1 | Phosphoserine Ref.11 | ||||||
| Modified residue | 189 | 1 | Phosphoserine Ref.8 Ref.9 Ref.10 | ||||||
| Modified residue | 190 | 1 | Phosphoserine Ref.9 Ref.11 | ||||||
| Modified residue | 191 | 1 | Phosphoserine Ref.11 | ||||||
| Modified residue | 194 | 1 | Phosphoserine Ref.10 Ref.11 | ||||||
| Modified residue | 197 | 1 | Phosphoserine Ref.11 | ||||||
| Modified residue | 199 | 1 | Phosphoserine Ref.11 | ||||||
| Modified residue | 262 | 1 | Phosphoserine Ref.11 | ||||||
| Modified residue | 305 | 1 | Phosphothreonine; by ATR | ||||||
| Modified residue | 397 | 1 | Phosphoserine Ref.11 | ||||||
| Modified residue | 399 | 1 | Phosphoserine Ref.11 | ||||||
| Modified residue | 400 | 1 | Phosphotyrosine Ref.11 | ||||||
| Modified residue | 402 | 1 | Phosphoserine Ref.11 | ||||||
| Modified residue | 454 | 1 | Phosphoserine Ref.11 | ||||||
| Modified residue | 466 | 1 | Phosphoserine Ref.7 Ref.8 Ref.11 | ||||||
| Modified residue | 526 | 1 | Phosphotyrosine Ref.11 | ||||||
| Modified residue | 541 | 1 | Phosphoserine Ref.7 | ||||||
| Modified residue | 579 | 1 | Phosphoserine Ref.11 | ||||||
| Modified residue | 629 | 1 | Phosphoserine Ref.11 | ||||||
| Modified residue | 636 | 1 | Phosphoserine Ref.10 Ref.11 | ||||||
| Modified residue | 638 | 1 | Phosphoserine Ref.7 Ref.10 Ref.11 | ||||||
| Modified residue | 640 | 1 | Phosphoserine Ref.10 Ref.11 | ||||||
| Modified residue | 653 | 1 | Phosphoserine Ref.10 Ref.11 | ||||||
| Modified residue | 655 | 1 | Phosphoserine Ref.7 Ref.10 Ref.11 | ||||||
Experimental info | |||||||||
| Mutagenesis | 305 | 1 | T → A: No interaction with RAD53. Ref.6 | ||||||
Sequences
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References
| « Hide 'large scale' references | |
| [1] | "Sequencing and functional analysis of a 32,560 bp segment on the left arm of yeast chromosome II. Identification of 26 open reading frames, including the KIP1 and SEC17 genes." Scherens B., el Bakkoury M., Vierendeels F., Dubois E., Messenguy F. Yeast 9:1355-1371(1993) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c. |
| [2] | "Complete DNA sequence of yeast chromosome II." Feldmann H., Aigle M., Aljinovic G., Andre B., Baclet M.C., Barthe C., Baur A., Becam A.-M., Biteau N., Boles E., Brandt T., Brendel M., Brueckner M., Bussereau F., Christiansen C., Contreras R., Crouzet M., Cziepluch C. Kleine K.EMBO J. 13:5795-5809(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204508 / S288c. |
| [3] | Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c. |
| [4] | "Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS]. |
| [5] | "Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS]. |
| [6] | "Mdt1, a novel Rad53 FHA1 domain-interacting protein, modulates DNA damage tolerance and G(2)/M cell cycle progression in Saccharomyces cerevisiae." Pike B.L., Yongkiettrakul S., Tsai M.-D., Heierhorst J. Mol. Cell. Biol. 24:2779-2788(2004) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, INTERACTION WITH RAD53, PHOSPHORYLATION, MUTAGENESIS OF THR-305. |
| [7] | "Quantitative phosphoproteomics applied to the yeast pheromone signaling pathway." Gruhler A., Olsen J.V., Mohammed S., Mortensen P., Faergeman N.J., Mann M., Jensen O.N. Mol. Cell. Proteomics 4:310-327(2005) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-466; SER-541; SER-638 AND SER-655, MASS SPECTROMETRY. Strain: YAL6B. |
| [8] | "Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae." Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P. J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-189 AND SER-466, MASS SPECTROMETRY. Strain: ADR376. |
| [9] | "Analysis of phosphorylation sites on proteins from Saccharomyces cerevisiae by electron transfer dissociation (ETD) mass spectrometry." Chi A., Huttenhower C., Geer L.Y., Coon J.J., Syka J.E.P., Bai D.L., Shabanowitz J., Burke D.J., Troyanskaya O.G., Hunt D.F. Proc. Natl. Acad. Sci. U.S.A. 104:2193-2198(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-189 AND SER-190, MASS SPECTROMETRY. |
| [10] | "Proteome-wide identification of in vivo targets of DNA damage checkpoint kinases." Smolka M.B., Albuquerque C.P., Chen S.H., Zhou H. Proc. Natl. Acad. Sci. U.S.A. 104:10364-10369(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-189; SER-194; SER-636; SER-638; SER-640; SER-653 AND SER-655, MASS SPECTROMETRY. |
| [11] | "A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-56; SER-65; SER-190; SER-191; SER-194; SER-197; SER-199; SER-262; SER-397; SER-399; TYR-400; SER-402; SER-454; SER-466; TYR-526; SER-579; SER-629; SER-636; SER-638; SER-640; SER-653 AND SER-655, MASS SPECTROMETRY. |
| + | Additional computationally mapped references. |
Cross-references
Sequence databases | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| EMBL GenBank DDBJ | Z23261 Genomic DNA. Translation: CAA80795.1. Z35812 Genomic DNA. Translation: CAA84871.1. BK006936 Genomic DNA. Translation: DAA07068.1. | ||||||||||||
| PIR | S39836. | ||||||||||||
| RefSeq | NP_009502.1. NM_001178291.1. | ||||||||||||
3D structure databases | |||||||||||||
| PDBe RCSB PDB PDBj |
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| ProteinModelPortal | P34217. | ||||||||||||
| ModBase | Search... | ||||||||||||
Protein-protein interaction databases | |||||||||||||
| DIP | DIP-1905N. | ||||||||||||
| IntAct | P34217. 42 interactions. | ||||||||||||
| MINT | MINT-388641. | ||||||||||||
| STRING | 4932.YBL051C. | ||||||||||||
Proteomic databases | |||||||||||||
| PaxDb | P34217. | ||||||||||||
| PeptideAtlas | P34217. | ||||||||||||
Protocols and materials databases | |||||||||||||
| StructuralBiologyKnowledgebase | Search... | ||||||||||||
Genome annotation databases | |||||||||||||
| EnsemblFungi | YBL051C; YBL051C; YBL051C. | ||||||||||||
| GeneID | 852229. | ||||||||||||
| KEGG | sce:YBL051C. | ||||||||||||
Organism-specific databases | |||||||||||||
| CYGD | YBL051c. | ||||||||||||
| SGD | S000000147. PIN4. | ||||||||||||
Phylogenomic databases | |||||||||||||
| eggNOG | COG0724. | ||||||||||||
| HOGENOM | HOG000115549. | ||||||||||||
| OMA | KKMLPQA. | ||||||||||||
| OrthoDB | EOG4QG0PB. | ||||||||||||
Gene expression databases | |||||||||||||
| Genevestigator | P34217. | ||||||||||||
| GermOnline | YBL051C. Saccharomyces cerevisiae. | ||||||||||||
Family and domain databases | |||||||||||||
| Gene3D | 3.30.70.330. 1 hit. | ||||||||||||
| InterPro | IPR012677. Nucleotide-bd_a/b_plait. IPR000504. RRM_dom. [Graphical view] | ||||||||||||
| SMART | SM00360. RRM. 1 hit. [Graphical view] | ||||||||||||
| PROSITE | PS50102. RRM. 1 hit. [Graphical view] | ||||||||||||
| ProtoNet | Search... | ||||||||||||
Other | |||||||||||||
| EvolutionaryTrace | P34217. | ||||||||||||
| NextBio | 970760. | ||||||||||||
Entry information
| Entry name | PIN4_YEAST | ||||||||
| Accession | Primary (citable) accession number: P34217 Secondary accession number(s): D6VPU8 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation program | Fungal Protein Annotation Program | ||||||||
Relevant documents
| Yeast Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD |
| Yeast chromosome II Yeast (Saccharomyces cerevisiae) chromosome II: entries and gene names |
| PDB cross-references Index of Protein Data Bank (PDB) cross-references |
| SIMILARITY comments Index of protein domains and families |

Clusters with
