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Protein

Serine/threonine-protein kinase chk1

Gene

chk1

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Serine/threonine-protein kinase which is required for checkpoint-mediated cell cycle arrest and activation of DNA repair in response to the presence of DNA damage or unreplicated DNA (PubMed:8497322). May also negatively regulate cell cycle progression during unperturbed cell cycles. Binds to and phosphorylates CDC25. This leads to negative regulation of CDC25 and prevents mitotic entry (PubMed:9278510).2 Publications

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei38ATPPROSITE-ProRule annotation1
Active sitei137Proton acceptorPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi16 – 24ATPPROSITE-ProRule annotation9

GO - Molecular functioni

GO - Biological processi

  • meiotic recombination checkpoint Source: PomBase
  • mitotic DNA replication checkpoint Source: PomBase
  • mitotic G2 DNA damage checkpoint Source: PomBase
  • negative regulation of G2/M transition of mitotic cell cycle Source: PomBase
  • negative regulation of transcription from RNA polymerase II promoter by transcription factor localization involved in response to DNA damage checkpoint signaling Source: PomBase
  • peptidyl-serine phosphorylation Source: GO_Central
  • peptidyl-threonine phosphorylation Source: GO_Central
  • signal transduction involved in mitotic DNA damage checkpoint Source: PomBase
  • signal transduction involved in mitotic G2 DNA damage checkpoint Source: PomBase

Keywordsi

Molecular functionKinase, Serine/threonine-protein kinase, Transferase
Biological processCell cycle, DNA damage
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi2.7.11.1 5613
ReactomeiR-SPO-176187 Activation of ATR in response to replication stress
R-SPO-5693616 Presynaptic phase of homologous DNA pairing and strand exchange
R-SPO-69601 Ubiquitin Mediated Degradation of Phosphorylated Cdc25A

Names & Taxonomyi

Protein namesi
Recommended name:
Serine/threonine-protein kinase chk1 (EC:2.7.11.1)
Alternative name(s):
Checkpoint kinase 1
Gene namesi
Name:chk1
Synonyms:rad27
ORF Names:SPCC1259.13
OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifieri284812 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
Proteomesi
  • UP000002485 Componenti: Chromosome III

Organism-specific databases

EuPathDBiFungiDB:SPCC1259.13
PomBaseiSPCC1259.13 chk1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000858561 – 496Serine/threonine-protein kinase chk1Add BLAST496

Post-translational modificationi

Phosphorylated.

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP34208
PaxDbiP34208
PRIDEiP34208

PTM databases

iPTMnetiP34208

Interactioni

Subunit structurei

Interacts with crb2 (PubMed:9407031, PubMed:14739927, PubMed:22792081). Interacts with rad3 (PubMed:14739927). Interacts with rfp1 (PubMed:17502373).4 Publications

Binary interactionsi

Show more details

Protein-protein interaction databases

BioGridi275659, 101 interactors
IntActiP34208, 9 interactors
STRINGi4896.SPCC1259.13.1

Structurei

3D structure databases

ProteinModelPortaliP34208
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini10 – 272Protein kinasePROSITE-ProRule annotationAdd BLAST263

Sequence similaritiesi

Phylogenomic databases

HOGENOMiHOG000216658
InParanoidiP34208
KOiK02216
OMAiFAVKFIH
OrthoDBiEOG092C35VZ
PhylomeDBiP34208

Family and domain databases

CDDicd14069 STKc_Chk1, 1 hit
InterProiView protein in InterPro
IPR034670 Chk1_catalytic_dom
IPR011009 Kinase-like_dom_sf
IPR000719 Prot_kinase_dom
IPR017441 Protein_kinase_ATP_BS
IPR008271 Ser/Thr_kinase_AS
PfamiView protein in Pfam
PF00069 Pkinase, 1 hit
SMARTiView protein in SMART
SM00220 S_TKc, 1 hit
SUPFAMiSSF56112 SSF56112, 1 hit
PROSITEiView protein in PROSITE
PS00107 PROTEIN_KINASE_ATP, 1 hit
PS50011 PROTEIN_KINASE_DOM, 1 hit
PS00108 PROTEIN_KINASE_ST, 1 hit

Sequencei

Sequence statusi: Complete.

P34208-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAQKLDNFPY HIGREIGTGA FASVRLCYDD NAKIYAVKFV NKKHATSCMN
60 70 80 90 100
AGVWARRMAS EIQLHKLCNG HKNIIHFYNT AENPQWRWVV LEFAQGGDLF
110 120 130 140 150
DKIEPDVGID EDVAQFYFAQ LMEGISFMHS KGVAHRDLKP ENILLDYNGN
160 170 180 190 200
LKISDFGFAS LFSYKGKSRL LNSPVGSPPY AAPEITQQYD GSKVDVWSCG
210 220 230 240 250
IILFALLLGN TPWDEAISNT GDYLLYKKQC ERPSYHPWNL LSPGAYSIIT
260 270 280 290 300
GMLRSDPFKR YSVKHVVQHP WLTSSTPFRT KNGNCADPVA LASRLMLKLR
310 320 330 340 350
IDLDKPRLAS SRASQNDSGF SMTQPAFKKN DQKELDRVEV YGALSQPVQL
360 370 380 390 400
NKNIDVTEIL EKDPSLSQFC ENEGFIKRLA KKAKNFYEIC PPERLTRFYS
410 420 430 440 450
RASRETIIDH LYDSLRLLAI SVTMKYVRNQ TILYVNLHDK RKCLLQGVIE
460 470 480 490
LTNLGHNLEL INFIKRNGDP LEWRKFFKNV VSSIGKPIVL TDVSQN
Length:496
Mass (Da):56,506
Last modified:June 1, 1994 - v2
Checksum:i0DEB8E10541F7942
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L13742 Genomic DNA Translation: AAA79758.1
U37421 mRNA Translation: AAA80539.1
CU329672 Genomic DNA Translation: CAA22551.1
D85545 Genomic DNA Translation: BAA20374.1
PIRiS33597
RefSeqiNP_588070.1, NM_001023062.2

Genome annotation databases

EnsemblFungiiSPCC1259.13.1; SPCC1259.13.1:pep; SPCC1259.13
KEGGispo:SPCC1259.13

Similar proteinsi

Entry informationi

Entry nameiCHK1_SCHPO
AccessioniPrimary (citable) accession number: P34208
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: June 1, 1994
Last modified: June 20, 2018
This is version 161 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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