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Protein

Serine/threonine-protein kinase chk1

Gene

chk1

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Serine/threonine-protein kinase which is required for checkpoint-mediated cell cycle arrest and activation of DNA repair in response to the presence of DNA damage or unreplicated DNA (PubMed:8497322). May also negatively regulate cell cycle progression during unperturbed cell cycles. Binds to and phosphorylates CDC25. This leads to negative regulation of CDC25 and prevents mitotic entry (PubMed:9278510).2 Publications

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei38 – 381ATPPROSITE-ProRule annotation
Active sitei137 – 1371Proton acceptorPROSITE-ProRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi16 – 249ATPPROSITE-ProRule annotation

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • protein serine/threonine kinase activity Source: PomBase

GO - Biological processi

  • mitotic DNA damage checkpoint Source: PomBase
  • mitotic G2 DNA damage checkpoint Source: PomBase
  • negative regulation of G2/M transition of mitotic cell cycle Source: PomBase
  • negative regulation of transcription from RNA polymerase II promoter by transcription factor localization involved in response to DNA damage checkpoint signaling Source: PomBase
  • peptidyl-serine phosphorylation Source: PomBase
  • signal transduction involved in mitotic DNA damage checkpoint Source: PomBase
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Biological processi

Cell cycle, DNA damage

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi2.7.11.1. 5613.
ReactomeiR-SPO-176187. Activation of ATR in response to replication stress.
R-SPO-5693616. Presynaptic phase of homologous DNA pairing and strand exchange.
R-SPO-69601. Ubiquitin Mediated Degradation of Phosphorylated Cdc25A.

Names & Taxonomyi

Protein namesi
Recommended name:
Serine/threonine-protein kinase chk1 (EC:2.7.11.1)
Alternative name(s):
Checkpoint kinase 1
Gene namesi
Name:chk1
Synonyms:rad27
ORF Names:SPCC1259.13
OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifieri284812 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
Proteomesi
  • UP000002485 Componenti: Chromosome III

Organism-specific databases

EuPathDBiFungiDB:SPCC1259.13.
PomBaseiSPCC1259.13. chk1.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: PomBase
  • cytosol Source: PomBase
  • nuclear chromatin Source: PomBase
  • nucleoplasm Source: PomBase
  • nucleus Source: PomBase
  • site of double-strand break Source: PomBase
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 496496Serine/threonine-protein kinase chk1PRO_0000085856Add
BLAST

Post-translational modificationi

Phosphorylated.

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP34208.

PTM databases

iPTMnetiP34208.

Interactioni

Subunit structurei

Interacts with crb2 (PubMed:9407031, PubMed:14739927, PubMed:22792081). Interacts with rad3 (PubMed:14739927). Interacts with rfp1 (PubMed:17502373).4 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
crb2P870744EBI-768535,EBI-768448
rfp1O138263EBI-768535,EBI-3647269

Protein-protein interaction databases

BioGridi275659. 97 interactions.
IntActiP34208. 9 interactions.
MINTiMINT-1179497.

Structurei

3D structure databases

ProteinModelPortaliP34208.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini10 – 272263Protein kinasePROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 protein kinase domain.PROSITE-ProRule annotation

Phylogenomic databases

HOGENOMiHOG000216658.
InParanoidiP34208.
KOiK02216.
OMAiPSMSQFS.
OrthoDBiEOG7VB2Q7.
PhylomeDBiP34208.

Family and domain databases

InterProiIPR020636. Ca/CaM-dep_Ca-dep_prot_Kinase.
IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PANTHERiPTHR24347. PTHR24347. 2 hits.
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P34208-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAQKLDNFPY HIGREIGTGA FASVRLCYDD NAKIYAVKFV NKKHATSCMN
60 70 80 90 100
AGVWARRMAS EIQLHKLCNG HKNIIHFYNT AENPQWRWVV LEFAQGGDLF
110 120 130 140 150
DKIEPDVGID EDVAQFYFAQ LMEGISFMHS KGVAHRDLKP ENILLDYNGN
160 170 180 190 200
LKISDFGFAS LFSYKGKSRL LNSPVGSPPY AAPEITQQYD GSKVDVWSCG
210 220 230 240 250
IILFALLLGN TPWDEAISNT GDYLLYKKQC ERPSYHPWNL LSPGAYSIIT
260 270 280 290 300
GMLRSDPFKR YSVKHVVQHP WLTSSTPFRT KNGNCADPVA LASRLMLKLR
310 320 330 340 350
IDLDKPRLAS SRASQNDSGF SMTQPAFKKN DQKELDRVEV YGALSQPVQL
360 370 380 390 400
NKNIDVTEIL EKDPSLSQFC ENEGFIKRLA KKAKNFYEIC PPERLTRFYS
410 420 430 440 450
RASRETIIDH LYDSLRLLAI SVTMKYVRNQ TILYVNLHDK RKCLLQGVIE
460 470 480 490
LTNLGHNLEL INFIKRNGDP LEWRKFFKNV VSSIGKPIVL TDVSQN
Length:496
Mass (Da):56,506
Last modified:June 1, 1994 - v2
Checksum:i0DEB8E10541F7942
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L13742 Genomic DNA. Translation: AAA79758.1.
U37421 mRNA. Translation: AAA80539.1.
CU329672 Genomic DNA. Translation: CAA22551.1.
D85545 Genomic DNA. Translation: BAA20374.1.
PIRiS33597.
RefSeqiNP_588070.1. NM_001023062.2.

Genome annotation databases

EnsemblFungiiSPCC1259.13.1; SPCC1259.13.1:pep; SPCC1259.13.
GeneIDi2539087.
KEGGispo:SPCC1259.13.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L13742 Genomic DNA. Translation: AAA79758.1.
U37421 mRNA. Translation: AAA80539.1.
CU329672 Genomic DNA. Translation: CAA22551.1.
D85545 Genomic DNA. Translation: BAA20374.1.
PIRiS33597.
RefSeqiNP_588070.1. NM_001023062.2.

3D structure databases

ProteinModelPortaliP34208.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi275659. 97 interactions.
IntActiP34208. 9 interactions.
MINTiMINT-1179497.

PTM databases

iPTMnetiP34208.

Proteomic databases

MaxQBiP34208.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiSPCC1259.13.1; SPCC1259.13.1:pep; SPCC1259.13.
GeneIDi2539087.
KEGGispo:SPCC1259.13.

Organism-specific databases

EuPathDBiFungiDB:SPCC1259.13.
PomBaseiSPCC1259.13. chk1.

Phylogenomic databases

HOGENOMiHOG000216658.
InParanoidiP34208.
KOiK02216.
OMAiPSMSQFS.
OrthoDBiEOG7VB2Q7.
PhylomeDBiP34208.

Enzyme and pathway databases

BRENDAi2.7.11.1. 5613.
ReactomeiR-SPO-176187. Activation of ATR in response to replication stress.
R-SPO-5693616. Presynaptic phase of homologous DNA pairing and strand exchange.
R-SPO-69601. Ubiquitin Mediated Degradation of Phosphorylated Cdc25A.

Miscellaneous databases

NextBioi20800259.
PROiP34208.

Family and domain databases

InterProiIPR020636. Ca/CaM-dep_Ca-dep_prot_Kinase.
IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PANTHERiPTHR24347. PTHR24347. 2 hits.
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Fission yeast chk1 protein kinase links the rad checkpoint pathway to cdc2."
    Walworth N., Davey S., Beach D.
    Nature 363:368-371(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA], FUNCTION.
  2. "Identification and characterization of new elements involved in checkpoint and feedback controls in fission yeast."
    Al-Khodairy F., Fotou E., Sheldrick K.S., Griffiths D.J.F., Lehmann A.R., Carr A.M.
    Mol. Biol. Cell 5:147-160(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA].
  3. "The genome sequence of Schizosaccharomyces pombe."
    Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.
    , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P.
    Nature 415:871-880(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 972 / ATCC 24843.
  4. "A ubiquitin-conjugating enzyme in fission yeast that is essential for the onset of anaphase in mitosis."
    Osaka F., Seino H., Seno T., Yamao F.
    Mol. Cell. Biol. 17:3388-3397(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-11.
    Strain: 972 / ATCC 24843.
  5. "Damage and replication checkpoint control in fission yeast is ensured by interactions of Crb2, a protein with BRCT motif, with Cut5 and Chk1."
    Saka Y., Esashi F., Matsusaka T., Mochida S., Yanagida M.
    Genes Dev. 11:3387-3400(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH CRB2.
    Strain: 972 / ATCC 24843.
  6. "Cdc25 mitotic inducer targeted by chk1 DNA damage checkpoint kinase."
    Furnari B., Rhind N., Russell P.
    Science 277:1495-1497(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  7. "Regulation of checkpoint kinases through dynamic interaction with Crb2."
    Mochida S., Esashi F., Aono N., Tamai K., O'Connell M.J., Yanagida M.
    EMBO J. 23:418-428(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH CRB2 AND RAD3.
  8. "Fission yeast Rnf4 homologs are required for DNA repair."
    Kosoy A., Calonge T.M., Outwin E.A., O'Connell M.J.
    J. Biol. Chem. 282:20388-20394(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH RFP1.
  9. "Phosphorylation-dependent interactions between Crb2 and Chk1 are essential for DNA damage checkpoint."
    Qu M., Yang B., Tao L., Yates J.R., Russell P., Dong M.Q., Du L.L.
    PLoS Genet. 8:E1002817-E1002817(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH CRB2.

Entry informationi

Entry nameiCHK1_SCHPO
AccessioniPrimary (citable) accession number: P34208
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: June 1, 1994
Last modified: May 11, 2016
This is version 144 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Schizosaccharomyces pombe
    Schizosaccharomyces pombe: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.