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Protein

Deoxyribodipyrimidine photo-lyase

Gene

phr

Organism
Carassius auratus (Goldfish)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Involved in repair of UV radiation-induced DNA damage. Catalyzes the light-dependent monomerization (300-600 nm) of cyclobutyl pyrimidine dimers (in cis-syn configuration), which are formed between adjacent bases on the same DNA strand upon exposure to ultraviolet radiation.

Catalytic activityi

Cyclobutadipyrimidine (in DNA) = 2 pyrimidine residues (in DNA).

Cofactori

FADBy similarityNote: Binds 1 FAD per subunit.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei333 – 3331DNABy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi478 – 4803FADBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Lyase

Keywords - Biological processi

DNA damage, DNA repair

Keywords - Ligandi

Chromophore, DNA-binding, FAD, Flavoprotein

Names & Taxonomyi

Protein namesi
Recommended name:
Deoxyribodipyrimidine photo-lyase (EC:4.1.99.3)
Alternative name(s):
DNA photolyase
Photoreactivating enzyme
Gene namesi
Name:phr
OrganismiCarassius auratus (Goldfish)
Taxonomic identifieri7957 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiActinopterygiiNeopterygiiTeleosteiOstariophysiCypriniformesCyprinidaeCarassius

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 556556Deoxyribodipyrimidine photo-lyasePRO_0000085119Add
BLAST

Expressioni

Inductioni

By visible light.

Structurei

3D structure databases

ProteinModelPortaliP34205.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini108 – 240133Photolyase/cryptochrome alpha/betaAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni378 – 3869Interaction with DNABy similarity
Regioni452 – 4532Interaction with DNABy similarity

Sequence similaritiesi

Belongs to the DNA photolyase class-2 family.Curated

Phylogenomic databases

HOVERGENiHBG008186.

Family and domain databases

Gene3Di3.40.50.620. 1 hit.
InterProiIPR005101. Cryptochr/Photolyase_FAD-bd.
IPR008148. DNA_photolyase_2.
IPR032673. DNA_photolyase_2_CS.
IPR006050. DNA_photolyase_N.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PANTHERiPTHR10211:SF0. PTHR10211:SF0. 1 hit.
PfamiPF00875. DNA_photolyase. 1 hit.
[Graphical view]
SUPFAMiSSF48173. SSF48173. 1 hit.
SSF52425. SSF52425. 1 hit.
TIGRFAMsiTIGR00591. phr2. 1 hit.
PROSITEiPS01083. DNA_PHOTOLYASES_2_1. 1 hit.
PS01084. DNA_PHOTOLYASES_2_2. 1 hit.
PS51645. PHR_CRY_ALPHA_BETA. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P34205-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSGECMLHIR LFSTNLYIRS TLLRSVSDPN TLLHYCSMSA NKRKLKRQRE
60 70 80 90 100
SPDSGGGKQP RLAEGRARES GWLLREVNEL RRAAQGCEVN KKRLRYLSDT
110 120 130 140 150
QKIKQGSDGF LYWMSRDQRV QDNWALIYAQ QLALAEKLPL HICFCLVPRY
160 170 180 190 200
LDATYRQYAF MLKGLQEVAK ECKSLDIQFH LLSGEPGQNL PSFVEKWKFG
210 220 230 240 250
AVVTDFNPLR IPLQWIETVK KHLPADVPFI QVDAHNVVPC WEASGKLEYG
260 270 280 290 300
ARTIRGKITK LLPEFLTEIP LVDTHPHSAS RAAEPVDWEE VLSSLEVERS
310 320 330 340 350
VGEVDWAQPG TSGGMNMLES FIDQRLRLFA THRNNPNYDA LSHLSPWIHT
360 370 380 390 400
GQLSAQRVVK QVKREKNASE SVASFIEELV VRRELADNFC FYNPSYDNIS
410 420 430 440 450
GAYDWAKKTL QDHAKDSRQY LYTKEQLENA KTHDQLWNAA QRQLVSEGKM
460 470 480 490 500
HGFLRMYWAK KILEWTASPE EALSIAIYLN DRLSLDGCDP NGYVGCMWSI
510 520 530 540 550
CGIHDQGWAE RPIFGKIRFM NYAGCKRKFD VAQFERKYTA VKENSNKDSK

KSSSKN
Length:556
Mass (Da):63,868
Last modified:February 1, 1994 - v1
Checksum:i28318FB406DC5646
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D11391 mRNA. Translation: BAA01987.1.
PIRiA45098.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D11391 mRNA. Translation: BAA01987.1.
PIRiA45098.

3D structure databases

ProteinModelPortaliP34205.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Phylogenomic databases

HOVERGENiHBG008186.

Family and domain databases

Gene3Di3.40.50.620. 1 hit.
InterProiIPR005101. Cryptochr/Photolyase_FAD-bd.
IPR008148. DNA_photolyase_2.
IPR032673. DNA_photolyase_2_CS.
IPR006050. DNA_photolyase_N.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PANTHERiPTHR10211:SF0. PTHR10211:SF0. 1 hit.
PfamiPF00875. DNA_photolyase. 1 hit.
[Graphical view]
SUPFAMiSSF48173. SSF48173. 1 hit.
SSF52425. SSF52425. 1 hit.
TIGRFAMsiTIGR00591. phr2. 1 hit.
PROSITEiPS01083. DNA_PHOTOLYASES_2_1. 1 hit.
PS01084. DNA_PHOTOLYASES_2_2. 1 hit.
PS51645. PHR_CRY_ALPHA_BETA. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Visible light-inducible photolyase gene from the goldfish Carassius auratus."
    Yasuhira S., Yasui A.
    J. Biol. Chem. 267:25644-25647(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].

Entry informationi

Entry nameiPHR_CARAU
AccessioniPrimary (citable) accession number: P34205
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: February 1, 1994
Last modified: May 11, 2016
This is version 79 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.