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P34185

- TOP1_SYNE7

UniProt

P34185 - TOP1_SYNE7

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Protein
DNA topoisomerase 1
Gene
topA, Synpcc7942_1416
Organism
Synechococcus elongatus (strain PCC 7942) (Anacystis nidulans R2)
Status
Reviewed - Annotation score: 4 out of 5 - Protein inferred from homologyi

Functioni

Releases the supercoiling and torsional tension of DNA, which is introduced during the DNA replication and transcription, by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand then undergoes passage around the unbroken strand, thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone By similarity.UniRule annotation

Catalytic activityi

ATP-independent breakage of single-stranded DNA, followed by passage and rejoining.UniRule annotation

Cofactori

Magnesium. Binds two Mg2+ per subunit By similarity.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi8 – 81Magnesium 1; catalytic By similarity
Sitei32 – 321Interaction with DNA By similarity
Metal bindingi95 – 951Magnesium 1; catalytic By similarity
Metal bindingi95 – 951Magnesium 2 By similarity
Metal bindingi97 – 971Magnesium 2 By similarity
Sitei151 – 1511Interaction with DNA By similarity
Sitei152 – 1521Interaction with DNA By similarity
Sitei155 – 1551Interaction with DNA By similarity
Sitei160 – 1601Interaction with DNA By similarity
Sitei167 – 1671Interaction with DNA By similarity
Active sitei320 – 3201O-(5'-phospho-DNA)-tyrosine intermediate By similarity
Sitei322 – 3221Interaction with DNA By similarity
Sitei515 – 5151Interaction with DNA By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. DNA binding Source: UniProtKB-KW
  3. DNA topoisomerase type I activity Source: UniProtKB-HAMAP
  4. magnesium ion binding Source: UniProtKB-HAMAP
Complete GO annotation...

GO - Biological processi

  1. DNA topological change Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Isomerase, Topoisomerase

Keywords - Ligandi

DNA-binding, Magnesium, Metal-binding

Enzyme and pathway databases

BioCyciSYNEL:SYNPCC7942_1416-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
DNA topoisomerase 1 (EC:5.99.1.2)
Alternative name(s):
DNA topoisomerase I
Omega-protein
Relaxing enzyme
Swivelase
Untwisting enzyme
Gene namesi
Name:topA
Ordered Locus Names:Synpcc7942_1416
OrganismiSynechococcus elongatus (strain PCC 7942) (Anacystis nidulans R2)
Taxonomic identifieri1140 [NCBI]
Taxonomic lineageiBacteriaCyanobacteriaOscillatoriophycideaeChroococcalesSynechococcus
ProteomesiUP000002717: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 883883DNA topoisomerase 1UniRule annotation
PRO_0000145167Add
BLAST

Interactioni

Subunit structurei

Monomer.

Protein-protein interaction databases

STRINGi1140.Synpcc7942_1416.

Structurei

3D structure databases

ProteinModelPortaliP34185.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini2 – 126125Toprim
Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni175 – 1806Interaction with DNA By similarity

Sequence similaritiesi

Contains 1 Toprim domain.

Phylogenomic databases

eggNOGiCOG1754.
HOGENOMiHOG000004020.
KOiK03168.
OrthoDBiEOG6S7XQ9.

Family and domain databases

Gene3Di1.10.460.10. 2 hits.
2.70.20.10. 2 hits.
3.40.50.140. 1 hit.
HAMAPiMF_00952. Topoisom_1_prok.
InterProiIPR000380. Topo_IA.
IPR003601. Topo_IA_2.
IPR023406. Topo_IA_AS.
IPR013497. Topo_IA_cen.
IPR013824. Topo_IA_cen_sub1.
IPR013825. Topo_IA_cen_sub2.
IPR023405. Topo_IA_core_domain.
IPR003602. Topo_IA_DNA-bd.
IPR005733. TopoI_bac-type.
IPR028612. Topoisom_1_IA.
IPR025589. Toprim_C_rpt.
IPR006171. Toprim_domain.
[Graphical view]
PANTHERiPTHR11390. PTHR11390. 1 hit.
PfamiPF01131. Topoisom_bac. 1 hit.
PF01751. Toprim. 1 hit.
PF13368. Toprim_C_rpt. 3 hits.
[Graphical view]
PRINTSiPR00417. PRTPISMRASEI.
SMARTiSM00437. TOP1Ac. 1 hit.
SM00436. TOP1Bc. 1 hit.
SM00493. TOPRIM. 1 hit.
[Graphical view]
SUPFAMiSSF56712. SSF56712. 1 hit.
TIGRFAMsiTIGR01051. topA_bact. 1 hit.
PROSITEiPS00396. TOPOISOMERASE_I_PROK. 1 hit.
PS50880. TOPRIM. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P34185-1 [UniParc]FASTAAdd to Basket

« Hide

MPKLVIVESP TKARTIRNYL PKDYRVEASM GHVRDLPQSA SDIPTELKGE    50
KWSNLGVDVE NNFAPLYIVP KDKKKIVKTL KDALKDADEL ILATDEDREG 100
ESISWHLLQL LQPRVPTKRM VFHEITQEAI QAALKNCRDV DQRLVHAQET 150
RRILDRLVGY TLSPLLWKKI AWGLSAGRVQ SVAVRLLVQR ERARRAFRQG 200
SYWDLKAQLT VEAGQFEAKL WTLAGQRLAT GNDFDESTGQ IIAGRQVCLL 250
DQQEAEALRD RLQTQPWQVK SLEEKPTTRK PAPPFTTSTL QQESNRKLRL 300
SARETMRVAQ SLYERGFITY MRTDSVHLSQ QAIEAARSCV EQMYGKNYLS 350
PQPRQFTTKS KNAQEAHEAI RPAGNTFRLP QETGLSGAEF ALYDLIWKRT 400
IASQMAEARQ TMLSVLLEVD NAEFRASGKR IDFPGFFRAY VEGSDDPDAA 450
LEDREILLPA LKVGDRPTCQ ELAAIGHETQ PPARYTEASL VKMLENEGIG 500
RPSTYASIIG TIVDRGYAQL VSNTLTPTFT AFAVTALLEQ HFPDLVDTSF 550
SARMEQSLDD ISNGEVDWLP YLSQFYRGDR GLEEQVKLRE SEIDPAAART 600
VALEGLPAKV RIGRFGAYLE AEADGEPIKA NLPKELTPAD LDVQRVETLL 650
RQKTEGPDQL GTHPETDEPI YLLTGAYGPY VQLGAATEEK PKPKRASLPK 700
GMSLETISLE QAVGLLSLPR TLGEHPETGR RIQAGLGRFG PYVVCDLGGG 750
EKDYRSLKAD DDVLTIDLDR ALELLAQPKK SRGRGKEPIR EVGLHPDDQA 800
PIQIFEGPYG LYLKHGKVNA SLPEDEKPET ISLETAVAAL AAKAGQAKAK 850
GGRRSTGTPK SGETKARTTK TTKKTTTRRT TSR 883
Length:883
Mass (Da):98,296
Last modified:April 18, 2006 - v2
Checksum:iC90DAC6EE1202B9A
GO

Sequence cautioni

The sequence CAA51086.1 differs from that shown. Reason: Frameshift at several positions.

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti101 – 1022ES → KV in CAA51086. 1 Publication
Sequence conflicti392 – 3921L → V in CAA51086. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X72391 Genomic DNA. Translation: CAA51086.1. Frameshift.
CP000100 Genomic DNA. Translation: ABB57446.1.
PIRiS32158.
RefSeqiYP_400433.1. NC_007604.1.

Genome annotation databases

EnsemblBacteriaiABB57446; ABB57446; Synpcc7942_1416.
GeneIDi3774129.
KEGGisyf:Synpcc7942_1416.
PATRICi23788179. VBISynElo51371_1616.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X72391 Genomic DNA. Translation: CAA51086.1 . Frameshift.
CP000100 Genomic DNA. Translation: ABB57446.1 .
PIRi S32158.
RefSeqi YP_400433.1. NC_007604.1.

3D structure databases

ProteinModelPortali P34185.
ModBasei Search...

Protein-protein interaction databases

STRINGi 1140.Synpcc7942_1416.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ABB57446 ; ABB57446 ; Synpcc7942_1416 .
GeneIDi 3774129.
KEGGi syf:Synpcc7942_1416.
PATRICi 23788179. VBISynElo51371_1616.

Phylogenomic databases

eggNOGi COG1754.
HOGENOMi HOG000004020.
KOi K03168.
OrthoDBi EOG6S7XQ9.

Enzyme and pathway databases

BioCyci SYNEL:SYNPCC7942_1416-MONOMER.

Family and domain databases

Gene3Di 1.10.460.10. 2 hits.
2.70.20.10. 2 hits.
3.40.50.140. 1 hit.
HAMAPi MF_00952. Topoisom_1_prok.
InterProi IPR000380. Topo_IA.
IPR003601. Topo_IA_2.
IPR023406. Topo_IA_AS.
IPR013497. Topo_IA_cen.
IPR013824. Topo_IA_cen_sub1.
IPR013825. Topo_IA_cen_sub2.
IPR023405. Topo_IA_core_domain.
IPR003602. Topo_IA_DNA-bd.
IPR005733. TopoI_bac-type.
IPR028612. Topoisom_1_IA.
IPR025589. Toprim_C_rpt.
IPR006171. Toprim_domain.
[Graphical view ]
PANTHERi PTHR11390. PTHR11390. 1 hit.
Pfami PF01131. Topoisom_bac. 1 hit.
PF01751. Toprim. 1 hit.
PF13368. Toprim_C_rpt. 3 hits.
[Graphical view ]
PRINTSi PR00417. PRTPISMRASEI.
SMARTi SM00437. TOP1Ac. 1 hit.
SM00436. TOP1Bc. 1 hit.
SM00493. TOPRIM. 1 hit.
[Graphical view ]
SUPFAMi SSF56712. SSF56712. 1 hit.
TIGRFAMsi TIGR01051. topA_bact. 1 hit.
PROSITEi PS00396. TOPOISOMERASE_I_PROK. 1 hit.
PS50880. TOPRIM. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The genomic region of rbcLS in Synechococcus sp. PCC 7942 contains genes involved in the ability to grow under low CO2 concentration and in chlorophyll biosynthesis."
    Ronen-Tarazi M., Lieman-Hurwitz J., Gabay C., Orus M.I., Kaplan A.
    Plant Physiol. 108:1461-1469(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "Complete sequence of chromosome 1 of Synechococcus elongatus PCC 7942."
    US DOE Joint Genome Institute
    Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina T., Hammon N., Israni S., Pitluck S., Schmutz J., Larimer F., Land M., Kyrpides N., Lykidis A., Richardson P.
    Submitted (AUG-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: PCC 7942.

Entry informationi

Entry nameiTOP1_SYNE7
AccessioniPrimary (citable) accession number: P34185
Secondary accession number(s): Q31NC3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: April 18, 2006
Last modified: September 3, 2014
This is version 101 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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