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P34185 (TOP1_SYNE7) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 98. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
DNA topoisomerase 1

EC=5.99.1.2
Alternative name(s):
DNA topoisomerase I
Omega-protein
Relaxing enzyme
Swivelase
Untwisting enzyme
Gene names
Name:topA
Ordered Locus Names:Synpcc7942_1416
OrganismSynechococcus elongatus (strain PCC 7942) (Anacystis nidulans R2) [Complete proteome] [HAMAP]
Taxonomic identifier1140 [NCBI]
Taxonomic lineageBacteriaCyanobacteriaOscillatoriophycideaeChroococcalesSynechococcus

Protein attributes

Sequence length883 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Releases the supercoiling and torsional tension of DNA, which is introduced during the DNA replication and transcription, by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand then undergoes passage around the unbroken strand, thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone By similarity. HAMAP-Rule MF_00952

Catalytic activity

ATP-independent breakage of single-stranded DNA, followed by passage and rejoining. HAMAP-Rule MF_00952

Cofactor

Magnesium. Binds two Mg2+ per subunit By similarity. HAMAP-Rule MF_00952

Subunit structure

Monomer.

Sequence similarities

Belongs to the type IA topoisomerase family.

Contains 1 Toprim domain.

Sequence caution

The sequence CAA51086.1 differs from that shown. Reason: Frameshift at several positions.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 883883DNA topoisomerase 1 HAMAP-Rule MF_00952
PRO_0000145167

Regions

Domain2 – 126125Toprim
Region175 – 1806Interaction with DNA By similarity

Sites

Active site3201O-(5'-phospho-DNA)-tyrosine intermediate By similarity
Metal binding81Magnesium 1; catalytic By similarity
Metal binding951Magnesium 1; catalytic By similarity
Metal binding951Magnesium 2 By similarity
Metal binding971Magnesium 2 By similarity
Site321Interaction with DNA By similarity
Site1511Interaction with DNA By similarity
Site1521Interaction with DNA By similarity
Site1551Interaction with DNA By similarity
Site1601Interaction with DNA By similarity
Site1671Interaction with DNA By similarity
Site3221Interaction with DNA By similarity
Site5151Interaction with DNA By similarity

Experimental info

Sequence conflict101 – 1022ES → KV in CAA51086. Ref.1
Sequence conflict3921L → V in CAA51086. Ref.1

Sequences

Sequence LengthMass (Da)Tools
P34185 [UniParc].

Last modified April 18, 2006. Version 2.
Checksum: C90DAC6EE1202B9A

FASTA88398,296
        10         20         30         40         50         60 
MPKLVIVESP TKARTIRNYL PKDYRVEASM GHVRDLPQSA SDIPTELKGE KWSNLGVDVE 

        70         80         90        100        110        120 
NNFAPLYIVP KDKKKIVKTL KDALKDADEL ILATDEDREG ESISWHLLQL LQPRVPTKRM 

       130        140        150        160        170        180 
VFHEITQEAI QAALKNCRDV DQRLVHAQET RRILDRLVGY TLSPLLWKKI AWGLSAGRVQ 

       190        200        210        220        230        240 
SVAVRLLVQR ERARRAFRQG SYWDLKAQLT VEAGQFEAKL WTLAGQRLAT GNDFDESTGQ 

       250        260        270        280        290        300 
IIAGRQVCLL DQQEAEALRD RLQTQPWQVK SLEEKPTTRK PAPPFTTSTL QQESNRKLRL 

       310        320        330        340        350        360 
SARETMRVAQ SLYERGFITY MRTDSVHLSQ QAIEAARSCV EQMYGKNYLS PQPRQFTTKS 

       370        380        390        400        410        420 
KNAQEAHEAI RPAGNTFRLP QETGLSGAEF ALYDLIWKRT IASQMAEARQ TMLSVLLEVD 

       430        440        450        460        470        480 
NAEFRASGKR IDFPGFFRAY VEGSDDPDAA LEDREILLPA LKVGDRPTCQ ELAAIGHETQ 

       490        500        510        520        530        540 
PPARYTEASL VKMLENEGIG RPSTYASIIG TIVDRGYAQL VSNTLTPTFT AFAVTALLEQ 

       550        560        570        580        590        600 
HFPDLVDTSF SARMEQSLDD ISNGEVDWLP YLSQFYRGDR GLEEQVKLRE SEIDPAAART 

       610        620        630        640        650        660 
VALEGLPAKV RIGRFGAYLE AEADGEPIKA NLPKELTPAD LDVQRVETLL RQKTEGPDQL 

       670        680        690        700        710        720 
GTHPETDEPI YLLTGAYGPY VQLGAATEEK PKPKRASLPK GMSLETISLE QAVGLLSLPR 

       730        740        750        760        770        780 
TLGEHPETGR RIQAGLGRFG PYVVCDLGGG EKDYRSLKAD DDVLTIDLDR ALELLAQPKK 

       790        800        810        820        830        840 
SRGRGKEPIR EVGLHPDDQA PIQIFEGPYG LYLKHGKVNA SLPEDEKPET ISLETAVAAL 

       850        860        870        880 
AAKAGQAKAK GGRRSTGTPK SGETKARTTK TTKKTTTRRT TSR 

« Hide

References

« Hide 'large scale' references
[1]"The genomic region of rbcLS in Synechococcus sp. PCC 7942 contains genes involved in the ability to grow under low CO2 concentration and in chlorophyll biosynthesis."
Ronen-Tarazi M., Lieman-Hurwitz J., Gabay C., Orus M.I., Kaplan A.
Plant Physiol. 108:1461-1469(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"Complete sequence of chromosome 1 of Synechococcus elongatus PCC 7942."
US DOE Joint Genome Institute
Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina T., Hammon N., Israni S., Pitluck S., Schmutz J., Larimer F., Land M., Kyrpides N., Lykidis A., Richardson P.
Submitted (AUG-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: PCC 7942.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X72391 Genomic DNA. Translation: CAA51086.1. Frameshift.
CP000100 Genomic DNA. Translation: ABB57446.1.
PIRS32158.
RefSeqYP_400433.1. NC_007604.1.

3D structure databases

ProteinModelPortalP34185.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING1140.Synpcc7942_1416.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABB57446; ABB57446; Synpcc7942_1416.
GeneID3774129.
KEGGsyf:Synpcc7942_1416.
PATRIC23788179. VBISynElo51371_1616.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG1754.
HOGENOMHOG000004020.
KOK03168.
OrthoDBEOG6S7XQ9.
ProtClustDBPRK07561.

Enzyme and pathway databases

BioCycSYNEL:SYNPCC7942_1416-MONOMER.

Family and domain databases

Gene3D1.10.460.10. 2 hits.
2.70.20.10. 2 hits.
3.40.50.140. 1 hit.
HAMAPMF_00952. Topoisom_1_prok.
InterProIPR000380. Topo_IA.
IPR003601. Topo_IA_2.
IPR023406. Topo_IA_AS.
IPR013497. Topo_IA_cen.
IPR013824. Topo_IA_cen_sub1.
IPR013825. Topo_IA_cen_sub2.
IPR023405. Topo_IA_core_domain.
IPR003602. Topo_IA_DNA-bd.
IPR005733. TopoI_bac-type.
IPR028612. Topoisom_1_IA.
IPR025589. Toprim_C_rpt.
IPR006171. Toprim_domain.
[Graphical view]
PANTHERPTHR11390. PTHR11390. 1 hit.
PfamPF01131. Topoisom_bac. 1 hit.
PF01751. Toprim. 1 hit.
PF13368. Toprim_C_rpt. 3 hits.
[Graphical view]
PRINTSPR00417. PRTPISMRASEI.
SMARTSM00437. TOP1Ac. 1 hit.
SM00436. TOP1Bc. 1 hit.
SM00493. TOPRIM. 1 hit.
[Graphical view]
SUPFAMSSF56712. SSF56712. 1 hit.
TIGRFAMsTIGR01051. topA_bact. 1 hit.
PROSITEPS00396. TOPOISOMERASE_I_PROK. 1 hit.
PS50880. TOPRIM. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameTOP1_SYNE7
AccessionPrimary (citable) accession number: P34185
Secondary accession number(s): Q31NC3
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: April 18, 2006
Last modified: February 19, 2014
This is version 98 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families