Reviewed, UniProtKB/Swiss-Prot P34185 (TOP1_SYNE7)

Names and origin

Protein names

Recommended name:
    DNA topoisomerase 1
    EC=5.99.1.2
Alternative name(s):
    DNA topoisomerase I
    Omega-protein
    Relaxing enzyme
    Untwisting enzyme
    Swivelase

Gene names

Name:	topA
Ordered Locus Names:	Synpcc7942_1416

Organism

Synechococcus elongatus (strain PCC 7942) (Anacystis nidulans R2) [Complete proteome] [HAMAP]

Taxonomic identifier

1140 [NCBI]

Taxonomic lineage

Bacteria › Cyanobacteria › Chroococcales › Synechococcus

Protein attributes

Sequence length	883 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	The reaction catalyzed by topoisomerases leads to the conversion of one topological isomer of DNA to another.
Catalytic activity	ATP-independent breakage of single-stranded DNA, followed by passage and rejoining.
Subunit structure	Monomer.
Miscellaneous	When a topoisomerase transiently breaks a DNA backbone bond, it simultaneously forms a protein-DNA link, in which a tyrosyl oxygen in the enzyme is joined to a DNA phosphorus at one end of the enzyme-severed DNA strand.
Sequence similarities	Belongs to the prokaryotic type I/III topoisomerase family.
Sequence caution	The sequence CAA51086.1 differs from that shown. Reason: Frameshift at several positions.

Ontologies

Keywords
Ligand	ATP-binding DNA-binding Nucleotide-binding
Molecular function	Isomerase Topoisomerase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	DNA topological change Inferred from electronic annotation. Source: InterPro DNA unwinding during replication Inferred from electronic annotation. Source: InterPro
Cellular component	chromosome Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW DNA topoisomerase type I activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 883	883	DNA topoisomerase 1		PRO_0000145167
Sites
	Active site	320	1	For DNA cleavage activity By similarity
Experimental info
	Sequence conflict	101 – 102	2	ES → KV in CAA51086. Ref.1
	Sequence conflict	392	1	L → V in CAA51086. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P34185-1 [UniParc]. Last modified April 18, 2006. Version 2. Checksum: C90DAC6EE1202B9A	FASTA	883	98,296
	10 20 30 40 50 60 MPKLVIVESP TKARTIRNYL PKDYRVEASM GHVRDLPQSA SDIPTELKGE KWSNLGVDVE 70 80 90 100 110 120 NNFAPLYIVP KDKKKIVKTL KDALKDADEL ILATDEDREG ESISWHLLQL LQPRVPTKRM 130 140 150 160 170 180 VFHEITQEAI QAALKNCRDV DQRLVHAQET RRILDRLVGY TLSPLLWKKI AWGLSAGRVQ 190 200 210 220 230 240 SVAVRLLVQR ERARRAFRQG SYWDLKAQLT VEAGQFEAKL WTLAGQRLAT GNDFDESTGQ 250 260 270 280 290 300 IIAGRQVCLL DQQEAEALRD RLQTQPWQVK SLEEKPTTRK PAPPFTTSTL QQESNRKLRL 310 320 330 340 350 360 SARETMRVAQ SLYERGFITY MRTDSVHLSQ QAIEAARSCV EQMYGKNYLS PQPRQFTTKS 370 380 390 400 410 420 KNAQEAHEAI RPAGNTFRLP QETGLSGAEF ALYDLIWKRT IASQMAEARQ TMLSVLLEVD 430 440 450 460 470 480 NAEFRASGKR IDFPGFFRAY VEGSDDPDAA LEDREILLPA LKVGDRPTCQ ELAAIGHETQ 490 500 510 520 530 540 PPARYTEASL VKMLENEGIG RPSTYASIIG TIVDRGYAQL VSNTLTPTFT AFAVTALLEQ 550 560 570 580 590 600 HFPDLVDTSF SARMEQSLDD ISNGEVDWLP YLSQFYRGDR GLEEQVKLRE SEIDPAAART 610 620 630 640 650 660 VALEGLPAKV RIGRFGAYLE AEADGEPIKA NLPKELTPAD LDVQRVETLL RQKTEGPDQL 670 680 690 700 710 720 GTHPETDEPI YLLTGAYGPY VQLGAATEEK PKPKRASLPK GMSLETISLE QAVGLLSLPR 730 740 750 760 770 780 TLGEHPETGR RIQAGLGRFG PYVVCDLGGG EKDYRSLKAD DDVLTIDLDR ALELLAQPKK 790 800 810 820 830 840 SRGRGKEPIR EVGLHPDDQA PIQIFEGPYG LYLKHGKVNA SLPEDEKPET ISLETAVAAL 850 860 870 880 AAKAGQAKAK GGRRSTGTPK SGETKARTTK TTKKTTTRRT TSR

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The genomic region of rbcLS in Synechococcus sp. PCC 7942 contains genes involved in the ability to grow under low CO2 concentration and in chlorophyll biosynthesis." Ronen-Tarazi M., Lieman-Hurwitz J., Gabay C., Orus M.I., Kaplan A. Plant Physiol. 108:1461-1469(1995) [PubMed: 7659748] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Complete sequence of chromosome 1 of Synechococcus elongatus PCC 7942." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina T., Hammon N., Israni S., Pitluck S., Schmutz J., Larimer F., Land M., Kyrpides N., Lykidis A., Richardson P. Submitted (AUG-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	X72391 Genomic DNA. Translation: CAA51086.1. Frameshift. CP000100 Genomic DNA. Translation: ABB57446.1.
PIR	S32158.
RefSeq	YP_400433.1.
3D structure databases
HSSP	HSSP built from PDB template 1ECL based on UniProtKB P06612.
ModBase	Search...
Protein-protein interaction databases
STRING	P34185.
Genome annotation databases
GeneID	3774129.
GenomeReviews	Gene locus Synpcc7942_1416 in contig CP000100_GR.
KEGG	syf:Synpcc7942_1416.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	P34185.
Enzyme and pathway databases
BioCyc	SELO1140:SYNPCC7942_1416-MON.
Family and domain databases
InterPro	IPR003601. Topo_IA_2. IPR013497. Topo_IA_cen. IPR013824. Topo_IA_cen_sub1. IPR013826. Topo_IA_cen_sub3. IPR000380. Topo_IA_core. IPR003602. Topo_IA_DNA_bd. IPR005733. TopoI_bac. IPR006154. Toprim_dom_subgr. IPR006171. Toprim_domain. [Graphical view]
Gene3D	G3DSA:1.10.460.10. Topo_IA_cen_sub1. 2 hits. G3DSA:1.10.290.10. Topo_IA_cen_sub3. 1 hit.
PANTHER	PTHR11390. Topo_IA. 1 hit.
Pfam	PF01131. Topoisom_bac. 1 hit. PF01751. Toprim. 1 hit. [Graphical view]
PRINTS	PR00417. PRTPISMRASEI.
SMART	SM00437. TOP1Ac. 1 hit. SM00436. TOP1Bc. 1 hit. SM00493. TOPRIM. 1 hit. [Graphical view]
TIGRFAMs	TIGR01051. topA_bact. 1 hit.
PROSITE	PS00396. TOPOISOMERASE_I_PROK. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

TOP1_SYNE7

Accession

Primary (citable) accession number: P34185
Secondary accession number(s): Q31NC3

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1994
Last sequence update:	April 18, 2006
Last modified:	September 1, 2009
This is version 61 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)