Reviewed, UniProtKB/Swiss-Prot P34184 (TOP1_BACPF)

Names and origin

Protein names

Recommended name:
    DNA topoisomerase 1
    EC=5.99.1.2
Alternative name(s):
    DNA topoisomerase I
    Omega-protein
    Relaxing enzyme
    Untwisting enzyme
    Swivelase

Gene names

Name:

topA

Organism

Bacillus pseudofirmus

Taxonomic identifier

79885 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

Protein attributes

Sequence length	505 AA.
Sequence status	Fragment.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	The reaction catalyzed by topoisomerases leads to the conversion of one topological isomer of DNA to another.
Catalytic activity	ATP-independent breakage of single-stranded DNA, followed by passage and rejoining.
Subunit structure	Monomer By similarity.
Miscellaneous	When a topoisomerase transiently breaks a DNA backbone bond, it simultaneously forms a protein-DNA link, in which a tyrosyl oxygen in the enzyme is joined to a DNA phosphorus at one end of the enzyme-severed DNA strand. Readthrough of the terminator UGA may occur between codons for Tyr-262 and Leu-263.
Sequence similarities	Belongs to the prokaryotic type I/III topoisomerase family.

Ontologies

Keywords
Ligand	ATP-binding DNA-binding Nucleotide-binding
Molecular function	Isomerase Topoisomerase
Gene Ontology (GO)
Biological process	DNA topological change Inferred from electronic annotation. Source: InterPro DNA unwinding during replication Inferred from electronic annotation. Source: InterPro
Cellular component	chromosome Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW DNA topoisomerase type I activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	‹1 – 505	›505	DNA topoisomerase 1		PRO_0000145140
Sites
	Active site	296	1	For DNA cleavage activity By similarity
Experimental info
	Non-terminal residue	1	1

Sequences

	Sequence		Length	Mass (Da)
	P34184-1 [UniParc]. Last modified February 1, 1994. Version 1. Checksum: 2E7A646B6395BDDD	FASTA	505	57,454
	10 20 30 40 50 60 IYVAPPSSLL PEGALICAAV GHILEFLEPG ELNEKYKSYS LDSLPIIIDL FQYKVVSDKK 70 80 90 100 110 120 EVLQRIKDTI FDKRVKTIIL ATDAAAEGEY IGRNILYRLH CKKTIKRLWT SSMTAFSIQK 130 140 150 160 170 180 AFSQLKADAE TLPLYYQAKA RAESDYMIGL TLSRAYGILL KEQGIVPHNT TISLGRVQTP 190 200 210 220 230 240 LLAEIVKRER LLEQFTAENF WTVKATFNNQ GNVYEGEWFH EKENRIFTEE QDEQLCELVR 250 260 270 280 290 300 NQSSTIMEMK EEMRTYQPPL LYLSTLQMDA GNAFGFKPAE TLKYAQSLYD KGYLSYPRTQ 310 320 330 340 350 360 DERITESDAR ELENNIQFLS GHDTSGALFP LPVSTLMNNK RYIGEVTDHH ALLITDKIPK 370 380 390 400 410 420 DKDLSEDEKS IYHLVVKRIL AAHYPDVAMS HKEIITKVMD RFTFRSKGKE LLSKGRHHII 430 440 450 460 470 480 PPTNENDIML PTLLKGSEGV VTDTLTTKSK TKPPNRYTSS SLIGFMKNAA QAIEDEDRKS 490 500 ISNLPLGTEA TRAGLTHLVR IEKIY

References

[1]

"A 1.6 kb region of Bacillus firmus OF4 DNA encodes a homolog of Escherichia coli and yeast DNA topoisomerases and may contain a translational readthrough of UGA." Ivey D.M., Cheng J., Krulwich T.A. Nucleic Acids Res. 20:4928-4928(1992) [PubMed: 1329032] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: OF4.

Cross-references

Sequence databases
EMBLGenBankDDBJ	Z14112 Genomic DNA. Translation: CAA78484.1. Different termination. Z14112 Genomic DNA. Translation: CAA78485.1. Different initiation.
PIR	S23866.
3D structure databases
ModBase	Search...
Enzyme and pathway databases
BRENDA	5.99.1.2. 291073.
Family and domain databases
InterPro	IPR003601. Topo_IA_2. IPR013497. Topo_IA_cen. IPR013824. Topo_IA_cen_sub1. IPR013826. Topo_IA_cen_sub3. IPR000380. Topo_IA_core. IPR003602. Topo_IA_DNA_bd. [Graphical view]
Gene3D	G3DSA:1.10.460.10. Topo_IA_cen_sub1. 1 hit. G3DSA:1.10.290.10. Topo_IA_cen_sub3. 1 hit.
PANTHER	PTHR11390. Topo_IA. 1 hit.
Pfam	PF01131. Topoisom_bac. 1 hit. [Graphical view]
PRINTS	PR00417. PRTPISMRASEI.
SMART	SM00437. TOP1Ac. 1 hit. SM00436. TOP1Bc. 1 hit. [Graphical view]
PROSITE	PS00396. TOPOISOMERASE_I_PROK. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

TOP1_BACPF

Accession

Primary (citable) accession number: P34184

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1994
Last sequence update:	February 1, 1994
Last modified:	June 16, 2009
This is version 55 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)