P34170 (OBP2_BOMMO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names	Recommended name: General odorant-binding protein 2 Short name=GOBP2
Organism	Bombyx mori (Silk moth) [Reference proteome]
Taxonomic identifier	7091 [NCBI]
Taxonomic lineage	cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Protostomia › Ecdysozoa › Panarthropoda › Arthropoda › Mandibulata › Pancrustacea › Hexapoda › Insecta › Dicondylia › Pterygota › Neoptera › Endopterygota › Amphiesmenoptera › Lepidoptera › Glossata › Neolepidoptera › Heteroneura › Ditrysia › Obtectomera › Bombycoidea › Bombyciformes › Bombycidae › Bombycinae › Bombyx

Protein attributes

Sequence length	160 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Present in the aqueous fluid surrounding olfactory sensory dendrites and are thought to aid in the capture and transport of hydrophobic odorants into and through this fluid.
Subunit structure	Homodimer Probable.
Tissue specificity	Detected in antenna (at protein level). Expressed at high levels in antenna. Ref.1 Ref.3
Sequence similarities	Belongs to the PBP/GOBP family.

Ontologies

Keywords
Biological process	Olfaction Sensory transduction Transport
Domain	Signal
PTM	Disulfide bond
Technical term	3D-structure Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	response to stimulus Inferred from electronic annotation. Source: UniProtKB-KW sensory perception of smell Inferred from electronic annotation. Source: UniProtKB-KW transport Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	odorant binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Signal peptide

1 – 20

20

Ref.2

Chain

21 – 160

140

General odorant-binding protein 2

PRO_0000012566

Amino acid modifications

Disulfide bond

38 ↔ 73

By similarity

Disulfide bond

69 ↔ 127

By similarity

Disulfide bond

116 ↔ 136

By similarity

Secondary structure

1

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160

Helix Strand Turn

Details...

Helix

21 – 31

11

Helix

32 – 34

3

Helix

35 – 42

8

Turn

46 – 48

3

Helix

52 – 56

5

Helix

65 – 77

13

Beta strand

85 – 87

3

Helix

89 – 97

9

Turn

100 – 102

3

Helix

103 – 119

17

Turn

120 – 122

3

Helix

126 – 143

18

Helix

150 – 155

6

Turn

156 – 158

3

Sequences

	Sequence		Length	Mass (Da)
	P34170 [UniParc]. Last modified November 1, 1997. Version 2. Checksum: 2DC95D3FD284C81A	FASTA	160	18,145
	10 20 30 40 50 60 MFSFLILVFV ASVADSVIGT AEVMSHVTAH FGKTLEECRE ESGLSVDILD EFKHFWSDDF 70 80 90 100 110 120 DVVHRELGCA IICMSNKFSL MDDDVRMHHV NMDEYIKGFP NGQVLAEKMV KLIHNCEKQF 130 140 150 160 DTETDDCTRV VKVAACFKKD SRKEGIAPEV AMIEAVIEKY

References

[1]	"Binding proteins from the antennae of Bombyx mori." Krieger J., von Nickisch-Rosenegk E., Mameli M., Pelosi P., Breer H. Insect Biochem. Mol. Biol. 26:297-307(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY. Tissue: Antenna.
[2]	"Odorant-binding-protein subfamilies associate with distinct classes of olfactory receptor neurons in insects." Vogt R.G., Prestwich G.D., Lerner M.R. J. Neurobiol. 22:74-84(1991) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 21-41. Tissue: Antenna.
[3]	"Characterisation of Bombyx mori odorant-binding proteins reveals that a general odorant-binding protein discriminates between sex pheromone components." Zhou J.J., Robertson G., He X., Dufour S., Hooper A.M., Pickett J.A., Keep N.H., Field L.M. J. Mol. Biol. 389:529-545(2009) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.4 ANGSTROMS) OF 20-160 IN COMPLEXES WITH BOMBYKOL; BOMBYKAL AND SYNTHETIC ANALOGS, TISSUE SPECIFICITY.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

X94989 mRNA. Translation: CAA64445.1.

RefSeq

NP_001037498.1. NM_001044033.1.

UniGene

Bmo.16.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2WC5	X-ray	1.90	A	20-160	[»]
2WC6	X-ray	1.90	A	20-160	[»]
2WCH	X-ray	1.70	A	20-160	[»]
2WCJ	X-ray	1.40	A	20-160	[»]
2WCK	X-ray	1.61	A	20-160	[»]
2WCL	X-ray	1.61	A	20-160	[»]
2WCM	X-ray	1.50	A	20-160	[»]

ProteinModelPortal

P34170.

ModBase

Search...

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblMetazoa

BGIBMGA012614-RA; BGIBMGA012614-TA; BGIBMGA012614.

GeneID

693052.

Organism-specific databases

CTD

693052.

Family and domain databases

Gene3D

1.10.238.20. 1 hit.

InterPro

IPR006072. Odorant/phero-bd_Lep.
IPR006170. PBP_GOBP.
IPR023316. PBP_GOBP_dom.
[Graphical view]

Pfam

PF01395. PBP_GOBP. 1 hit.
[Graphical view]

PIRSF

PIRSF015604. Odorant/phero_bd. 1 hit.

PRINTS

PR00484. PBPGOBP.

SMART

SM00708. PhBP. 1 hit.
[Graphical view]

SUPFAM

SSF47565. PBP_GOBP. 1 hit.

ProtoNet

Search...

Other

EvolutionaryTrace

P34170.

Entry information

Entry name

OBP2_BOMMO

Accession

Primary (citable) accession number: P34170
Secondary accession number(s): Q17226

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1994
Last sequence update:	November 1, 1997
Last modified:	May 1, 2013
This is version 72 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)