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P34167 (IF4B_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 119. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Eukaryotic translation initiation factor 4B

Short name=eIF-4B
Gene names
Name:TIF3
Synonyms:STM1, TIF42
Ordered Locus Names:YPR163C
ORF Names:P9325.6
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length436 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Involved in translation initiation. May be the homolog of mammalian eIF4B and be part of an RNA helicase. STM1/TIF3 is a non-essential gene.

Miscellaneous

Present with 24000 molecules/cell in log phase SD medium.

Sequence similarities

Contains 1 RRM (RNA recognition motif) domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 436436Eukaryotic translation initiation factor 4B
PRO_0000081618

Regions

Domain101 – 18383RRM
Repeat190 – 210211; approximate
Repeat211 – 232222
Repeat233 – 258263
Repeat259 – 284264
Repeat285 – 310265
Repeat311 – 340306
Repeat341 – 350107; truncated
Region190 – 3501617 X approximate tandem repeats

Amino acid modifications

Modified residue651Phosphoserine Ref.7 Ref.8
Modified residue711Phosphoserine Ref.7 Ref.8

Experimental info

Mutagenesis1061N → G, V or Y: Retention of function.
Mutagenesis1061N → S or P: Loss of function.
Mutagenesis1501A → S or C: Retention of function.
Mutagenesis1501A → Y, F, N or L: Loss of function.

Sequences

Sequence LengthMass (Da)Tools
P34167 [UniParc].

Last modified February 1, 1994. Version 1.
Checksum: 1DFE89D90BF7D677

FASTA43648,522
        10         20         30         40         50         60 
MAPPKKTVKK MDLTSFLNDD TFGSSWAEED VDLNKITIPI ETANANTIPL SELAHAKNNS 

        70         80         90        100        110        120 
NNTRSGGFGG SFGGRSRLDP ALGGGSSDRR EEYPVPDAPP YRAVINNIPW DITPEGVQAW 

       130        140        150        160        170        180 
VEDGLVKPEA VEEVVLPKNL RDPTRLKGNA FVTLKERADL VAVLKFNGTK LNERTVYVSV 

       190        200        210        220        230        240 
AAPRRGGGAD VDWSSARGSN FQGDGREDAP DLDWGAARGS NFRGPRRERE EVDIDWTAAR 

       250        260        270        280        290        300 
GSNFQGSSRP PRREREEVDI DWSAARGSNF QGSSRPPRRE REEPDIDWSA ARGSNFQSSS 

       310        320        330        340        350        360 
RPPRREREEP DIDWSAARGS NFQSSSRPPR REREKEEPAL DWGAARGAQF GKPQQTKNTY 

       370        380        390        400        410        420 
KDRSLTNKKT TDEQPKIQKS VYDVLRTEDD DEDEEAEKQN GDAKENKVDA AVEKLQDKTA 

       430 
QLTVEDGDNW EVVGKK 

« Hide

References

« Hide 'large scale' references
[1]"A Saccharomyces cerevisiae homologue of mammalian translation initiation factor 4B contributes to RNA helicase activity."
Altmann M., Mueller P.P., Wittmer B., Ruchti F., Lanker S., Trachsel H.
EMBO J. 12:3997-4003(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"A new yeast translation initiation factor suppresses a mutation in the eIF-4A RNA helicase."
Coppolecchia R., Buser P., Stotz A., Linder P.
EMBO J. 12:4005-4011(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], MUTAGENESIS.
[3]"The nucleotide sequence of Saccharomyces cerevisiae chromosome XVI."
Bussey H., Storms R.K., Ahmed A., Albermann K., Allen E., Ansorge W., Araujo R., Aparicio A., Barrell B.G., Badcock K., Benes V., Botstein D., Bowman S., Brueckner M., Carpenter J., Cherry J.M., Chung E., Churcher C.M. expand/collapse author list , Coster F., Davis K., Davis R.W., Dietrich F.S., Delius H., DiPaolo T., Dubois E., Duesterhoeft A., Duncan M., Floeth M., Fortin N., Friesen J.D., Fritz C., Goffeau A., Hall J., Hebling U., Heumann K., Hilbert H., Hillier L.W., Hunicke-Smith S., Hyman R.W., Johnston M., Kalman S., Kleine K., Komp C., Kurdi O., Lashkari D., Lew H., Lin A., Lin D., Louis E.J., Marathe R., Messenguy F., Mewes H.-W., Mirtipati S., Moestl D., Mueller-Auer S., Namath A., Nentwich U., Oefner P., Pearson D., Petel F.X., Pohl T.M., Purnelle B., Rajandream M.A., Rechmann S., Rieger M., Riles L., Roberts D., Schaefer M., Scharfe M., Scherens B., Schramm S., Schroeder M., Sdicu A.-M., Tettelin H., Urrestarazu L.A., Ushinsky S., Vierendeels F., Vissers S., Voss H., Walsh S.V., Wambutt R., Wang Y., Wedler E., Wedler H., Winnett E., Zhong W.-W., Zollner A., Vo D.H., Hani J.
Nature 387:103-105(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[4]Saccharomyces Genome Database
Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[5]"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae."
Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. expand/collapse author list , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J.
Genome Res. 17:536-543(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[6]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[7]"A multidimensional chromatography technology for in-depth phosphoproteome analysis."
Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-65 AND SER-71, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[8]"Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-65 AND SER-71, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X71996 Genomic DNA. Translation: CAA50870.1.
U25840 Genomic DNA. Translation: AAB68150.1.
AY693114 Genomic DNA. Translation: AAT93133.1.
BK006949 Genomic DNA. Translation: DAA11580.1.
PIRS37302.
RefSeqNP_015489.1. NM_001184260.1.

3D structure databases

ProteinModelPortalP34167.
SMRP34167. Positions 94-187.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid36336. 41 interactions.
DIPDIP-3806N.
IntActP34167. 5 interactions.
MINTMINT-406545.
STRING4932.YPR163C.

Proteomic databases

PaxDbP34167.
PeptideAtlasP34167.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYPR163C; YPR163C; YPR163C.
GeneID856292.
KEGGsce:YPR163C.

Organism-specific databases

CYGDYPR163c.
SGDS000006367. TIF3.

Phylogenomic databases

eggNOGNOG238591.
HOGENOMHOG000248309.
KOK03258.
OMADWSSARG.
OrthoDBEOG7D59Z7.

Enzyme and pathway databases

BioCycYEAST:G3O-34292-MONOMER.

Gene expression databases

GenevestigatorP34167.

Family and domain databases

Gene3D3.30.70.330. 1 hit.
InterProIPR012677. Nucleotide-bd_a/b_plait.
IPR000504. RRM_dom.
[Graphical view]
SMARTSM00360. RRM. 1 hit.
[Graphical view]
PROSITEPS50102. RRM. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio981635.

Entry information

Entry nameIF4B_YEAST
AccessionPrimary (citable) accession number: P34167
Secondary accession number(s): D6W4G4
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: February 1, 1994
Last modified: April 16, 2014
This is version 119 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome XVI

Yeast (Saccharomyces cerevisiae) chromosome XVI: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families

Translation initiation factors

List of translation initiation factor entries