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P34167

- IF4B_YEAST

UniProt

P34167 - IF4B_YEAST

Protein

Eukaryotic translation initiation factor 4B

Gene

TIF3

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 124 (01 Oct 2014)
      Sequence version 1 (01 Feb 1994)
      Previous versions | rss
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    Functioni

    Involved in translation initiation. May be the homolog of mammalian eIF4B and be part of an RNA helicase. STM1/TIF3 is a non-essential gene.

    GO - Molecular functioni

    1. nucleotide binding Source: InterPro
    2. ribosomal small subunit binding Source: SGD
    3. RNA binding Source: SGD
    4. RNA strand annealing activity Source: SGD
    5. RNA strand-exchange activity Source: SGD
    6. single-stranded RNA binding Source: SGD
    7. translation initiation factor activity Source: UniProtKB-KW

    GO - Biological processi

    1. cytoplasmic translation Source: SGD
    2. eukaryotic translation initiation factor 4F complex assembly Source: SGD
    3. formation of translation preinitiation complex Source: SGD
    4. translational initiation Source: SGD

    Keywords - Molecular functioni

    Initiation factor

    Keywords - Biological processi

    Protein biosynthesis

    Keywords - Ligandi

    RNA-binding

    Enzyme and pathway databases

    BioCyciYEAST:G3O-34292-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Eukaryotic translation initiation factor 4B
    Short name:
    eIF-4B
    Gene namesi
    Name:TIF3
    Synonyms:STM1, TIF42
    Ordered Locus Names:YPR163C
    ORF Names:P9325.6
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome XVI

    Organism-specific databases

    CYGDiYPR163c.
    SGDiS000006367. TIF3.

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi106 – 1061N → G, V or Y: Retention of function. 1 Publication
    Mutagenesisi106 – 1061N → S or P: Loss of function. 1 Publication
    Mutagenesisi150 – 1501A → S or C: Retention of function. 1 Publication
    Mutagenesisi150 – 1501A → Y, F, N or L: Loss of function. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 436436Eukaryotic translation initiation factor 4BPRO_0000081618Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei65 – 651Phosphoserine2 Publications
    Modified residuei71 – 711Phosphoserine2 Publications

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiP34167.
    PaxDbiP34167.
    PeptideAtlasiP34167.

    Expressioni

    Gene expression databases

    GenevestigatoriP34167.

    Interactioni

    Protein-protein interaction databases

    BioGridi36336. 42 interactions.
    DIPiDIP-3806N.
    IntActiP34167. 5 interactions.
    MINTiMINT-406545.
    STRINGi4932.YPR163C.

    Structurei

    3D structure databases

    ProteinModelPortaliP34167.
    SMRiP34167. Positions 94-187.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini101 – 18383RRMPROSITE-ProRule annotationAdd
    BLAST
    Repeati190 – 210211; approximateAdd
    BLAST
    Repeati211 – 232222Add
    BLAST
    Repeati233 – 258263Add
    BLAST
    Repeati259 – 284264Add
    BLAST
    Repeati285 – 310265Add
    BLAST
    Repeati311 – 340306Add
    BLAST
    Repeati341 – 350107; truncated

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni190 – 3501617 X approximate tandem repeatsAdd
    BLAST

    Sequence similaritiesi

    Contains 1 RRM (RNA recognition motif) domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Repeat

    Phylogenomic databases

    eggNOGiNOG238591.
    HOGENOMiHOG000248309.
    KOiK03258.
    OMAiDWSSARG.
    OrthoDBiEOG7D59Z7.

    Family and domain databases

    Gene3Di3.30.70.330. 1 hit.
    InterProiIPR012677. Nucleotide-bd_a/b_plait.
    IPR000504. RRM_dom.
    [Graphical view]
    SMARTiSM00360. RRM. 1 hit.
    [Graphical view]
    PROSITEiPS50102. RRM. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P34167-1 [UniParc]FASTAAdd to Basket

    « Hide

    MAPPKKTVKK MDLTSFLNDD TFGSSWAEED VDLNKITIPI ETANANTIPL    50
    SELAHAKNNS NNTRSGGFGG SFGGRSRLDP ALGGGSSDRR EEYPVPDAPP 100
    YRAVINNIPW DITPEGVQAW VEDGLVKPEA VEEVVLPKNL RDPTRLKGNA 150
    FVTLKERADL VAVLKFNGTK LNERTVYVSV AAPRRGGGAD VDWSSARGSN 200
    FQGDGREDAP DLDWGAARGS NFRGPRRERE EVDIDWTAAR GSNFQGSSRP 250
    PRREREEVDI DWSAARGSNF QGSSRPPRRE REEPDIDWSA ARGSNFQSSS 300
    RPPRREREEP DIDWSAARGS NFQSSSRPPR REREKEEPAL DWGAARGAQF 350
    GKPQQTKNTY KDRSLTNKKT TDEQPKIQKS VYDVLRTEDD DEDEEAEKQN 400
    GDAKENKVDA AVEKLQDKTA QLTVEDGDNW EVVGKK 436
    Length:436
    Mass (Da):48,522
    Last modified:February 1, 1994 - v1
    Checksum:i1DFE89D90BF7D677
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X71996 Genomic DNA. Translation: CAA50870.1.
    U25840 Genomic DNA. Translation: AAB68150.1.
    AY693114 Genomic DNA. Translation: AAT93133.1.
    BK006949 Genomic DNA. Translation: DAA11580.1.
    PIRiS37302.
    RefSeqiNP_015489.1. NM_001184260.1.

    Genome annotation databases

    EnsemblFungiiYPR163C; YPR163C; YPR163C.
    GeneIDi856292.
    KEGGisce:YPR163C.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X71996 Genomic DNA. Translation: CAA50870.1 .
    U25840 Genomic DNA. Translation: AAB68150.1 .
    AY693114 Genomic DNA. Translation: AAT93133.1 .
    BK006949 Genomic DNA. Translation: DAA11580.1 .
    PIRi S37302.
    RefSeqi NP_015489.1. NM_001184260.1.

    3D structure databases

    ProteinModelPortali P34167.
    SMRi P34167. Positions 94-187.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 36336. 42 interactions.
    DIPi DIP-3806N.
    IntActi P34167. 5 interactions.
    MINTi MINT-406545.
    STRINGi 4932.YPR163C.

    Proteomic databases

    MaxQBi P34167.
    PaxDbi P34167.
    PeptideAtlasi P34167.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YPR163C ; YPR163C ; YPR163C .
    GeneIDi 856292.
    KEGGi sce:YPR163C.

    Organism-specific databases

    CYGDi YPR163c.
    SGDi S000006367. TIF3.

    Phylogenomic databases

    eggNOGi NOG238591.
    HOGENOMi HOG000248309.
    KOi K03258.
    OMAi DWSSARG.
    OrthoDBi EOG7D59Z7.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-34292-MONOMER.

    Miscellaneous databases

    NextBioi 981635.

    Gene expression databases

    Genevestigatori P34167.

    Family and domain databases

    Gene3Di 3.30.70.330. 1 hit.
    InterProi IPR012677. Nucleotide-bd_a/b_plait.
    IPR000504. RRM_dom.
    [Graphical view ]
    SMARTi SM00360. RRM. 1 hit.
    [Graphical view ]
    PROSITEi PS50102. RRM. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "A Saccharomyces cerevisiae homologue of mammalian translation initiation factor 4B contributes to RNA helicase activity."
      Altmann M., Mueller P.P., Wittmer B., Ruchti F., Lanker S., Trachsel H.
      EMBO J. 12:3997-4003(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. "A new yeast translation initiation factor suppresses a mutation in the eIF-4A RNA helicase."
      Coppolecchia R., Buser P., Stotz A., Linder P.
      EMBO J. 12:4005-4011(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], MUTAGENESIS.
    3. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XVI."
      Bussey H., Storms R.K., Ahmed A., Albermann K., Allen E., Ansorge W., Araujo R., Aparicio A., Barrell B.G., Badcock K., Benes V., Botstein D., Bowman S., Brueckner M., Carpenter J., Cherry J.M., Chung E., Churcher C.M.
      , Coster F., Davis K., Davis R.W., Dietrich F.S., Delius H., DiPaolo T., Dubois E., Duesterhoeft A., Duncan M., Floeth M., Fortin N., Friesen J.D., Fritz C., Goffeau A., Hall J., Hebling U., Heumann K., Hilbert H., Hillier L.W., Hunicke-Smith S., Hyman R.W., Johnston M., Kalman S., Kleine K., Komp C., Kurdi O., Lashkari D., Lew H., Lin A., Lin D., Louis E.J., Marathe R., Messenguy F., Mewes H.-W., Mirtipati S., Moestl D., Mueller-Auer S., Namath A., Nentwich U., Oefner P., Pearson D., Petel F.X., Pohl T.M., Purnelle B., Rajandream M.A., Rechmann S., Rieger M., Riles L., Roberts D., Schaefer M., Scharfe M., Scherens B., Schramm S., Schroeder M., Sdicu A.-M., Tettelin H., Urrestarazu L.A., Ushinsky S., Vierendeels F., Vissers S., Voss H., Walsh S.V., Wambutt R., Wang Y., Wedler E., Wedler H., Winnett E., Zhong W.-W., Zollner A., Vo D.H., Hani J.
      Nature 387:103-105(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    4. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    5. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    6. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    7. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
      Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
      Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-65 AND SER-71, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    8. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
      Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
      Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-65 AND SER-71, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiIF4B_YEAST
    AccessioniPrimary (citable) accession number: P34167
    Secondary accession number(s): D6W4G4
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1994
    Last sequence update: February 1, 1994
    Last modified: October 1, 2014
    This is version 124 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 24000 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Translation initiation factors
      List of translation initiation factor entries
    2. SIMILARITY comments
      Index of protein domains and families
    3. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    4. Yeast chromosome XVI
      Yeast (Saccharomyces cerevisiae) chromosome XVI: entries and gene names

    External Data

    Dasty 3