P34167 (IF4B_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Eukaryotic translation initiation factor 4B
Short name=eIF-4B

Gene names

Name:	TIF3
Synonyms:	STM1, TIF42
Ordered Locus Names:	YPR163C
ORF Names:	P9325.6

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › Saccharomyces cerevisiae

Protein attributes

Sequence length	436 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Involved in translation initiation. May be the homolog of mammalian eIF4B and be part of a RNA helicase. STM1/TIF3 is a non-essential gene.
Miscellaneous	Present with 24000 molecules/cell in log phase SD medium.
Sequence similarities	Contains 1 RRM (RNA recognition motif) domain.

Ontologies

Keywords
Biological process	Protein biosynthesis
Domain	Repeat
Ligand	RNA-binding
Molecular function	Initiation factor
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cytoplasmic translation Inferred from mutant phenotype Ref.1. Source: SGD
Cellular_component	ribosome Traceable author statement PubMed 9841679. Source: SGD
Molecular_function	RNA strand annealing activity Inferred from direct assay PubMed 7543843. Source: SGD nucleotide binding Inferred from electronic annotation. Source: InterPro translation initiation factor activity Inferred from sequence or structural similarity Ref.2. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 436	436	Eukaryotic translation initiation factor 4B		PRO_0000081618
Regions
	Domain	101 – 183	83	RRM
	Repeat	190 – 210	21	1; approximate
	Repeat	211 – 232	22	2
	Repeat	233 – 258	26	3
	Repeat	259 – 284	26	4
	Repeat	285 – 310	26	5
	Repeat	311 – 340	30	6
	Repeat	341 – 350	10	7; truncated
	Region	190 – 350	161	7 X approximate tandem repeats
Amino acid modifications
	Modified residue	65	1	Phosphoserine Ref.8
	Modified residue	71	1	Phosphoserine Ref.7 Ref.8
	Modified residue	370	1	Phosphothreonine Ref.8
Experimental info
	Mutagenesis	106	1	N → G, V or Y: Retention of function.
	Mutagenesis	106	1	N → S or P: Loss of function.
	Mutagenesis	150	1	A → S or C: Retention of function.
	Mutagenesis	150	1	A → Y, F, N or L: Loss of function.

Sequences

	Sequence		Length	Mass (Da)
	P34167 [UniParc]. Last modified February 1, 1994. Version 1. Checksum: 1DFE89D90BF7D677	FASTA	436	48,522
	10 20 30 40 50 60 MAPPKKTVKK MDLTSFLNDD TFGSSWAEED VDLNKITIPI ETANANTIPL SELAHAKNNS 70 80 90 100 110 120 NNTRSGGFGG SFGGRSRLDP ALGGGSSDRR EEYPVPDAPP YRAVINNIPW DITPEGVQAW 130 140 150 160 170 180 VEDGLVKPEA VEEVVLPKNL RDPTRLKGNA FVTLKERADL VAVLKFNGTK LNERTVYVSV 190 200 210 220 230 240 AAPRRGGGAD VDWSSARGSN FQGDGREDAP DLDWGAARGS NFRGPRRERE EVDIDWTAAR 250 260 270 280 290 300 GSNFQGSSRP PRREREEVDI DWSAARGSNF QGSSRPPRRE REEPDIDWSA ARGSNFQSSS 310 320 330 340 350 360 RPPRREREEP DIDWSAARGS NFQSSSRPPR REREKEEPAL DWGAARGAQF GKPQQTKNTY 370 380 390 400 410 420 KDRSLTNKKT TDEQPKIQKS VYDVLRTEDD DEDEEAEKQN GDAKENKVDA AVEKLQDKTA 430 QLTVEDGDNW EVVGKK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"A Saccharomyces cerevisiae homologue of mammalian translation initiation factor 4B contributes to RNA helicase activity." Altmann M., Mueller P.P., Wittmer B., Ruchti F., Lanker S., Trachsel H. EMBO J. 12:3997-4003(1993) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"A new yeast translation initiation factor suppresses a mutation in the eIF-4A RNA helicase." Coppolecchia R., Buser P., Stotz A., Linder P. EMBO J. 12:4005-4011(1993) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], MUTAGENESIS.
[3]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XVI." Bussey H., Storms R.K., Ahmed A., Albermann K., Allen E., Ansorge W., Araujo R., Aparicio A., Barrell B.G., Badcock K., Benes V., Botstein D., Bowman S., Brueckner M., Carpenter J., Cherry J.M., Chung E., Churcher C.M. , Coster F., Davis K., Davis R.W., Dietrich F.S., Delius H., DiPaolo T., Dubois E., Duesterhoeft A., Duncan M., Floeth M., Fortin N., Friesen J.D., Fritz C., Goffeau A., Hall J., Hebling U., Heumann K., Hilbert H., Hillier L.W., Hunicke-Smith S., Hyman R.W., Johnston M., Kalman S., Kleine K., Komp C., Kurdi O., Lashkari D., Lew H., Lin A., Lin D., Louis E.J., Marathe R., Messenguy F., Mewes H.-W., Mirtipati S., Moestl D., Mueller-Auer S., Namath A., Nentwich U., Oefner P., Pearson D., Petel F.X., Pohl T.M., Purnelle B., Rajandream M.A., Rechmann S., Rieger M., Riles L., Roberts D., Schaefer M., Scharfe M., Scherens B., Schramm S., Schroeder M., Sdicu A.-M., Tettelin H., Urrestarazu L.A., Ushinsky S., Vierendeels F., Vissers S., Voss H., Walsh S.V., Wambutt R., Wang Y., Wedler E., Wedler H., Winnett E., Zhong W.-W., Zollner A., Vo D.H., Hani J. Nature 387:103-105(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[4]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[5]	"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae." Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J. Genome Res. 17:536-543(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[6]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[7]	"Quantitative phosphoproteomics applied to the yeast pheromone signaling pathway." Gruhler A., Olsen J.V., Mohammed S., Mortensen P., Faergeman N.J., Mann M., Jensen O.N. Mol. Cell. Proteomics 4:310-327(2005) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-71, MASS SPECTROMETRY. Strain: YAL6B.
[8]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-65; SER-71 AND THR-370, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X71996 Genomic DNA. Translation: CAA50870.1. U25840 Genomic DNA. Translation: AAB68150.1. AY693114 Genomic DNA. Translation: AAT93133.1. BK006949 Genomic DNA. Translation: DAA11580.1.
PIR	S37302.
RefSeq	NP_015489.1. NM_001184260.1.
3D structure databases
ProteinModelPortal	P34167.
SMR	P34167. Positions 96-186.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-3806N.
IntAct	P34167. 5 interactions.
MINT	MINT-406545.
STRING	4932.YPR163C.
Proteomic databases
PaxDb	P34167.
PeptideAtlas	P34167.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YPR163C; YPR163C; YPR163C.
GeneID	856292.
KEGG	sce:YPR163C.
Organism-specific databases
CYGD	YPR163c.
SGD	S000006367. TIF3.
Phylogenomic databases
eggNOG	NOG238591.
HOGENOM	HOG000248309.
KO	K03258.
OMA	DWSSARG.
OrthoDB	EOG47DDR7.
Gene expression databases
Genevestigator	P34167.
GermOnline	YPR163C. Saccharomyces cerevisiae.
Family and domain databases
Gene3D	3.30.70.330. 1 hit.
InterPro	IPR012677. Nucleotide-bd_a/b_plait. IPR000504. RRM_dom. [Graphical view]
SMART	SM00360. RRM. 1 hit. [Graphical view]
PROSITE	PS50102. RRM. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	981635.

Entry information

Entry name

IF4B_YEAST

Accession

Primary (citable) accession number: P34167
Secondary accession number(s): D6W4G4

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1994
Last sequence update:	February 1, 1994
Last modified:	May 1, 2013
This is version 111 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program