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Protein

Eukaryotic translation initiation factor 4B

Gene

TIF3

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Involved in translation initiation. May be the homolog of mammalian eIF4B and be part of an RNA helicase. STM1/TIF3 is a non-essential gene.

Miscellaneous

Present with 24000 molecules/cell in log phase SD medium.1 Publication

GO - Molecular functioni

  • ribosomal small subunit binding Source: SGD
  • RNA binding Source: SGD
  • RNA strand annealing activity Source: SGD
  • RNA strand-exchange activity Source: SGD
  • single-stranded RNA binding Source: SGD
  • translation initiation factor activity Source: GO_Central

GO - Biological processi

  • cytoplasmic translation Source: SGD
  • eukaryotic translation initiation factor 4F complex assembly Source: SGD
  • formation of translation preinitiation complex Source: SGD
  • translational initiation Source: SGD

Keywordsi

Molecular functionInitiation factor, RNA-binding
Biological processProtein biosynthesis

Enzyme and pathway databases

BioCyciYEAST:G3O-34292-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Eukaryotic translation initiation factor 4B
Short name:
eIF-4B
Gene namesi
Name:TIF3
Synonyms:STM1, TIF42
Ordered Locus Names:YPR163C
ORF Names:P9325.6
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XVI

Organism-specific databases

EuPathDBiFungiDB:YPR163C.
SGDiS000006367. TIF3.

Subcellular locationi

GO - Cellular componenti

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi106N → G, V or Y: Retention of function. 1 Publication1
Mutagenesisi106N → S or P: Loss of function. 1 Publication1
Mutagenesisi150A → S or C: Retention of function. 1 Publication1
Mutagenesisi150A → Y, F, N or L: Loss of function. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000816181 – 436Eukaryotic translation initiation factor 4BAdd BLAST436

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei65PhosphoserineCombined sources1
Modified residuei71PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP34167.
PRIDEiP34167.
TopDownProteomicsiP34167.

PTM databases

iPTMnetiP34167.

Interactioni

Protein-protein interaction databases

BioGridi36336. 45 interactors.
DIPiDIP-3806N.
IntActiP34167. 6 interactors.
MINTiMINT-406545.
STRINGi4932.YPR163C.

Structurei

3D structure databases

ProteinModelPortaliP34167.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini101 – 183RRMPROSITE-ProRule annotationAdd BLAST83
Repeati190 – 2101; approximateAdd BLAST21
Repeati211 – 2322Add BLAST22
Repeati233 – 2583Add BLAST26
Repeati259 – 2844Add BLAST26
Repeati285 – 3105Add BLAST26
Repeati311 – 3406Add BLAST30
Repeati341 – 3507; truncated10

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni190 – 3507 X approximate tandem repeatsAdd BLAST161

Keywords - Domaini

Repeat

Phylogenomic databases

HOGENOMiHOG000248309.
InParanoidiP34167.
KOiK03258.
OMAiEEPNLDW.
OrthoDBiEOG092C33YN.

Family and domain databases

InterProiView protein in InterPro
IPR000504. RRM_dom.
SMARTiView protein in SMART
SM00360. RRM. 1 hit.
SUPFAMiSSF54928. SSF54928. 1 hit.
PROSITEiView protein in PROSITE
PS50102. RRM. 1 hit.

Sequencei

Sequence statusi: Complete.

P34167-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAPPKKTVKK MDLTSFLNDD TFGSSWAEED VDLNKITIPI ETANANTIPL
60 70 80 90 100
SELAHAKNNS NNTRSGGFGG SFGGRSRLDP ALGGGSSDRR EEYPVPDAPP
110 120 130 140 150
YRAVINNIPW DITPEGVQAW VEDGLVKPEA VEEVVLPKNL RDPTRLKGNA
160 170 180 190 200
FVTLKERADL VAVLKFNGTK LNERTVYVSV AAPRRGGGAD VDWSSARGSN
210 220 230 240 250
FQGDGREDAP DLDWGAARGS NFRGPRRERE EVDIDWTAAR GSNFQGSSRP
260 270 280 290 300
PRREREEVDI DWSAARGSNF QGSSRPPRRE REEPDIDWSA ARGSNFQSSS
310 320 330 340 350
RPPRREREEP DIDWSAARGS NFQSSSRPPR REREKEEPAL DWGAARGAQF
360 370 380 390 400
GKPQQTKNTY KDRSLTNKKT TDEQPKIQKS VYDVLRTEDD DEDEEAEKQN
410 420 430
GDAKENKVDA AVEKLQDKTA QLTVEDGDNW EVVGKK
Length:436
Mass (Da):48,522
Last modified:February 1, 1994 - v1
Checksum:i1DFE89D90BF7D677
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X71996 Genomic DNA. Translation: CAA50870.1.
U25840 Genomic DNA. Translation: AAB68150.1.
AY693114 Genomic DNA. Translation: AAT93133.1.
BK006949 Genomic DNA. Translation: DAA11580.1.
PIRiS37302.
RefSeqiNP_015489.1. NM_001184260.1.

Genome annotation databases

EnsemblFungiiYPR163C; YPR163C; YPR163C.
GeneIDi856292.
KEGGisce:YPR163C.

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Entry informationi

Entry nameiIF4B_YEAST
AccessioniPrimary (citable) accession number: P34167
Secondary accession number(s): D6W4G4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: February 1, 1994
Last modified: July 5, 2017
This is version 149 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Translation initiation factors
    List of translation initiation factor entries
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XVI
    Yeast (Saccharomyces cerevisiae) chromosome XVI: entries and gene names