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P34166

- MFA2_YEAST

UniProt

P34166 - MFA2_YEAST

Protein

Mating hormone A-factor 2

Gene

MFA2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
  1. Functioni

    The active factor is excreted into the culture medium by haploid cells of the A mating type and acts on cells of the opposite mating type (type alpha). It mediates the conjugation process between the two types by inhibiting the initiation of DNA synthesis in type alpha cells and synchronizing them with type A.

    GO - Molecular functioni

    1. mating pheromone activity Source: SGD

    GO - Biological processi

    1. pheromone-dependent signal transduction involved in conjugation with cellular fusion Source: SGD

    Keywords - Molecular functioni

    Pheromone

    Enzyme and pathway databases

    BioCyciYEAST:G3O-33163-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Mating hormone A-factor 2
    Gene namesi
    Name:MFA2
    Ordered Locus Names:YNL145W
    ORF Names:N1204, N1787
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome XIV

    Organism-specific databases

    CYGDiYNL145w.
    SGDiS000005089. MFA2.

    Subcellular locationi

    Cell membrane Curated; Lipid-anchor Curated; Cytoplasmic side Curated

    GO - Cellular componenti

    1. extracellular region Source: SGD
    2. plasma membrane Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cell membrane, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Propeptidei1 – 23231 PublicationPRO_0000021693Add
    BLAST
    Peptidei24 – 3512Mating hormone A-factor 2PRO_0000021694Add
    BLAST
    Propeptidei36 – 383Removed in mature formPRO_0000021695

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei35 – 351Cysteine methyl ester1 Publication
    Lipidationi35 – 351S-farnesyl cysteine1 Publication

    Keywords - PTMi

    Lipoprotein, Methylation, Prenylation

    Expressioni

    Gene expression databases

    GenevestigatoriP34166.

    Interactioni

    Protein-protein interaction databases

    BioGridi35683. 63 interactions.
    DIPiDIP-2140N.
    IntActiP34166. 1 interaction.
    MINTiMINT-527449.
    STRINGi4932.YNL145W.

    Structurei

    3D structure databases

    ProteinModelPortaliP34166.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Phylogenomic databases

    KOiK11235.
    OrthoDBiEOG7T1RR3.

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P34166-1 [UniParc]FASTAAdd to Basket

    « Hide

    MQPITTASTQ ATQKDKSSEK KDNYIIKGLF WDPACVIA                38
    Length:38
    Mass (Da):4,229
    Last modified:February 1, 1994 - v1
    Checksum:iC71466AEA2F133C4
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U20823 Genomic DNA. Translation: AAA68602.1.
    U26204 Genomic DNA. Translation: AAA67688.1.
    Z46843 Genomic DNA. Translation: CAA86881.1.
    S48033 mRNA. Translation: AAD13854.1.
    Z71421 Genomic DNA. Translation: CAA96028.1.
    AY693189 Genomic DNA. Translation: AAT93208.1.
    BK006947 Genomic DNA. Translation: DAA10404.1.
    PIRiS55139.
    RefSeqiNP_014254.1. NM_001182983.1.

    Genome annotation databases

    EnsemblFungiiYNL145W; YNL145W; YNL145W.
    GeneIDi855577.
    KEGGisce:YNL145W.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U20823 Genomic DNA. Translation: AAA68602.1 .
    U26204 Genomic DNA. Translation: AAA67688.1 .
    Z46843 Genomic DNA. Translation: CAA86881.1 .
    S48033 mRNA. Translation: AAD13854.1 .
    Z71421 Genomic DNA. Translation: CAA96028.1 .
    AY693189 Genomic DNA. Translation: AAT93208.1 .
    BK006947 Genomic DNA. Translation: DAA10404.1 .
    PIRi S55139.
    RefSeqi NP_014254.1. NM_001182983.1.

    3D structure databases

    ProteinModelPortali P34166.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 35683. 63 interactions.
    DIPi DIP-2140N.
    IntActi P34166. 1 interaction.
    MINTi MINT-527449.
    STRINGi 4932.YNL145W.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YNL145W ; YNL145W ; YNL145W .
    GeneIDi 855577.
    KEGGi sce:YNL145W.

    Organism-specific databases

    CYGDi YNL145w.
    SGDi S000005089. MFA2.

    Phylogenomic databases

    KOi K11235.
    OrthoDBi EOG7T1RR3.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-33163-MONOMER.

    Miscellaneous databases

    NextBioi 979700.

    Gene expression databases

    Genevestigatori P34166.

    Family and domain databases

    ProtoNeti Search...

    Publicationsi

    1. "Structure of genes encoding precursors of the yeast peptide mating pheromone a-factor."
      Brake A.J., Brenner C., Najarian R., Laybourn P., Merryweather J.
      (In) Gething M.-J. (eds.); Protein transport and secretion, pp.103-108, Cold Spring Harbor Laboratory Press, New York (1985)
      Cited for: NUCLEOTIDE SEQUENCE.
    2. "The a-factor pheromone of Saccharomyces cerevisiae is essential for mating."
      Michaelis S., Herskowitz I.
      Mol. Cell. Biol. 8:1309-1318(1988) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE.
    3. "3'-UTR-dependent deadenylation by the yeast poly(A) nuclease."
      Lowell J.E., Rudner D.Z., Sachs A.B.
      Genes Dev. 6:2088-2099(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    4. "The sequence of 36.8 kb from the left arm of chromosome XIV reveals 24 complete open reading frames: 18 correspond to new genes, one of which encodes a protein similar to the human myotonic dystrophy kinase."
      Nasr F., Becam A.-M., Herbert C.J.
      Yeast 12:169-175(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE.
      Strain: ATCC 96604 / S288c / FY1679.
    5. "A 43.5 kb segment of yeast chromosome XIV, which contains MFA2, MEP2, CAP/SRV2, NAM9, FKB1/FPR1/RBP1, MOM22 and CPT1, predicts an adenosine deaminase gene and 14 new open reading frames."
      Mallet L., Bussereau F., Jacquet M.
      Yeast 11:1195-1209(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    6. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV and its evolutionary implications."
      Philippsen P., Kleine K., Poehlmann R., Duesterhoeft A., Hamberg K., Hegemann J.H., Obermaier B., Urrestarazu L.A., Aert R., Albermann K., Altmann R., Andre B., Baladron V., Ballesta J.P.G., Becam A.-M., Beinhauer J.D., Boskovic J., Buitrago M.J.
      , Bussereau F., Coster F., Crouzet M., D'Angelo M., Dal Pero F., De Antoni A., del Rey F., Doignon F., Domdey H., Dubois E., Fiedler T.A., Fleig U., Floeth M., Fritz C., Gaillardin C., Garcia-Cantalejo J.M., Glansdorff N., Goffeau A., Gueldener U., Herbert C.J., Heumann K., Heuss-Neitzel D., Hilbert H., Hinni K., Iraqui Houssaini I., Jacquet M., Jimenez A., Jonniaux J.-L., Karpfinger-Hartl L., Lanfranchi G., Lepingle A., Levesque H., Lyck R., Maftahi M., Mallet L., Maurer C.T.C., Messenguy F., Mewes H.-W., Moestl D., Nasr F., Nicaud J.-M., Niedenthal R.K., Pandolfo D., Pierard A., Piravandi E., Planta R.J., Pohl T.M., Purnelle B., Rebischung C., Remacha M.A., Revuelta J.L., Rinke M., Saiz J.E., Sartorello F., Scherens B., Sen-Gupta M., Soler-Mira A., Urbanus J.H.M., Valle G., Van Dyck L., Verhasselt P., Vierendeels F., Vissers S., Voet M., Volckaert G., Wach A., Wambutt R., Wedler H., Zollner A., Hani J.
      Nature 387:93-98(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    7. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    8. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    9. "Amino acid sequences of a-factor mating peptides from Saccharomyces cerevisiae."
      Betz R., Crabb J.W., Meyer H.E., Wittig R., Duntze W.
      J. Biol. Chem. 262:546-548(1987) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 24-35.
    10. "Structure of Saccharomyces cerevisiae mating hormone a-factor. Identification of S-farnesyl cysteine as a structural component."
      Anderegg R.J., Betz R., Carr S.A., Crabb J.W., Duntze W.
      J. Biol. Chem. 263:18236-18240(1988) [PubMed] [Europe PMC] [Abstract]
      Cited for: ISOPRENYLATION AT CYS-35, METHYLATION AT CYS-35.

    Entry informationi

    Entry nameiMFA2_YEAST
    AccessioniPrimary (citable) accession number: P34166
    Secondary accession number(s): D6W138
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1994
    Last sequence update: February 1, 1994
    Last modified: October 1, 2014
    This is version 112 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    2. Yeast chromosome XIV
      Yeast (Saccharomyces cerevisiae) chromosome XIV: entries and gene names

    External Data

    Dasty 3