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P34166

- MFA2_YEAST

UniProt

P34166 - MFA2_YEAST

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Protein

Mating hormone A-factor 2

Gene

MFA2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli

Functioni

The active factor is excreted into the culture medium by haploid cells of the A mating type and acts on cells of the opposite mating type (type alpha). It mediates the conjugation process between the two types by inhibiting the initiation of DNA synthesis in type alpha cells and synchronizing them with type A.

GO - Molecular functioni

  1. mating pheromone activity Source: SGD

GO - Biological processi

  1. pheromone-dependent signal transduction involved in conjugation with cellular fusion Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Pheromone

Enzyme and pathway databases

BioCyciYEAST:G3O-33163-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Mating hormone A-factor 2
Gene namesi
Name:MFA2
Ordered Locus Names:YNL145W
ORF Names:N1204, N1787
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome XIV

Organism-specific databases

CYGDiYNL145w.
SGDiS000005089. MFA2.

Subcellular locationi

Cell membrane Curated; Lipid-anchor Curated; Cytoplasmic side Curated

GO - Cellular componenti

  1. extracellular region Source: SGD
  2. plasma membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Propeptidei1 – 23231 PublicationPRO_0000021693Add
BLAST
Peptidei24 – 3512Mating hormone A-factor 2PRO_0000021694Add
BLAST
Propeptidei36 – 383Removed in mature formPRO_0000021695

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei35 – 351Cysteine methyl ester1 Publication
Lipidationi35 – 351S-farnesyl cysteine1 Publication

Keywords - PTMi

Lipoprotein, Methylation, Prenylation

Expressioni

Gene expression databases

GenevestigatoriP34166.

Interactioni

Protein-protein interaction databases

BioGridi35683. 64 interactions.
DIPiDIP-2140N.
IntActiP34166. 1 interaction.
MINTiMINT-527449.
STRINGi4932.YNL145W.

Structurei

3D structure databases

ProteinModelPortaliP34166.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Phylogenomic databases

InParanoidiP34166.
KOiK11235.
OrthoDBiEOG7T1RR3.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P34166-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30 
MQPITTASTQ ATQKDKSSEK KDNYIIKGLF WDPACVIA
Length:38
Mass (Da):4,229
Last modified:February 1, 1994 - v1
Checksum:iC71466AEA2F133C4
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U20823 Genomic DNA. Translation: AAA68602.1.
U26204 Genomic DNA. Translation: AAA67688.1.
Z46843 Genomic DNA. Translation: CAA86881.1.
S48033 mRNA. Translation: AAD13854.1.
Z71421 Genomic DNA. Translation: CAA96028.1.
AY693189 Genomic DNA. Translation: AAT93208.1.
BK006947 Genomic DNA. Translation: DAA10404.1.
PIRiS55139.
RefSeqiNP_014254.1. NM_001182983.1.

Genome annotation databases

EnsemblFungiiYNL145W; YNL145W; YNL145W.
GeneIDi855577.
KEGGisce:YNL145W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U20823 Genomic DNA. Translation: AAA68602.1 .
U26204 Genomic DNA. Translation: AAA67688.1 .
Z46843 Genomic DNA. Translation: CAA86881.1 .
S48033 mRNA. Translation: AAD13854.1 .
Z71421 Genomic DNA. Translation: CAA96028.1 .
AY693189 Genomic DNA. Translation: AAT93208.1 .
BK006947 Genomic DNA. Translation: DAA10404.1 .
PIRi S55139.
RefSeqi NP_014254.1. NM_001182983.1.

3D structure databases

ProteinModelPortali P34166.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 35683. 64 interactions.
DIPi DIP-2140N.
IntActi P34166. 1 interaction.
MINTi MINT-527449.
STRINGi 4932.YNL145W.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YNL145W ; YNL145W ; YNL145W .
GeneIDi 855577.
KEGGi sce:YNL145W.

Organism-specific databases

CYGDi YNL145w.
SGDi S000005089. MFA2.

Phylogenomic databases

InParanoidi P34166.
KOi K11235.
OrthoDBi EOG7T1RR3.

Enzyme and pathway databases

BioCyci YEAST:G3O-33163-MONOMER.

Miscellaneous databases

NextBioi 979700.

Gene expression databases

Genevestigatori P34166.

Family and domain databases

ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Structure of genes encoding precursors of the yeast peptide mating pheromone a-factor."
    Brake A.J., Brenner C., Najarian R., Laybourn P., Merryweather J.
    (In) Gething M.-J. (eds.); Protein transport and secretion, pp.103-108, Cold Spring Harbor Laboratory Press, New York (1985)
    Cited for: NUCLEOTIDE SEQUENCE.
  2. "The a-factor pheromone of Saccharomyces cerevisiae is essential for mating."
    Michaelis S., Herskowitz I.
    Mol. Cell. Biol. 8:1309-1318(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE.
  3. "3'-UTR-dependent deadenylation by the yeast poly(A) nuclease."
    Lowell J.E., Rudner D.Z., Sachs A.B.
    Genes Dev. 6:2088-2099(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  4. "The sequence of 36.8 kb from the left arm of chromosome XIV reveals 24 complete open reading frames: 18 correspond to new genes, one of which encodes a protein similar to the human myotonic dystrophy kinase."
    Nasr F., Becam A.-M., Herbert C.J.
    Yeast 12:169-175(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE.
    Strain: ATCC 96604 / S288c / FY1679.
  5. "A 43.5 kb segment of yeast chromosome XIV, which contains MFA2, MEP2, CAP/SRV2, NAM9, FKB1/FPR1/RBP1, MOM22 and CPT1, predicts an adenosine deaminase gene and 14 new open reading frames."
    Mallet L., Bussereau F., Jacquet M.
    Yeast 11:1195-1209(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  6. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV and its evolutionary implications."
    Philippsen P., Kleine K., Poehlmann R., Duesterhoeft A., Hamberg K., Hegemann J.H., Obermaier B., Urrestarazu L.A., Aert R., Albermann K., Altmann R., Andre B., Baladron V., Ballesta J.P.G., Becam A.-M., Beinhauer J.D., Boskovic J., Buitrago M.J.
    , Bussereau F., Coster F., Crouzet M., D'Angelo M., Dal Pero F., De Antoni A., del Rey F., Doignon F., Domdey H., Dubois E., Fiedler T.A., Fleig U., Floeth M., Fritz C., Gaillardin C., Garcia-Cantalejo J.M., Glansdorff N., Goffeau A., Gueldener U., Herbert C.J., Heumann K., Heuss-Neitzel D., Hilbert H., Hinni K., Iraqui Houssaini I., Jacquet M., Jimenez A., Jonniaux J.-L., Karpfinger-Hartl L., Lanfranchi G., Lepingle A., Levesque H., Lyck R., Maftahi M., Mallet L., Maurer C.T.C., Messenguy F., Mewes H.-W., Moestl D., Nasr F., Nicaud J.-M., Niedenthal R.K., Pandolfo D., Pierard A., Piravandi E., Planta R.J., Pohl T.M., Purnelle B., Rebischung C., Remacha M.A., Revuelta J.L., Rinke M., Saiz J.E., Sartorello F., Scherens B., Sen-Gupta M., Soler-Mira A., Urbanus J.H.M., Valle G., Van Dyck L., Verhasselt P., Vierendeels F., Vissers S., Voet M., Volckaert G., Wach A., Wambutt R., Wedler H., Zollner A., Hani J.
    Nature 387:93-98(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  7. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  8. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  9. "Amino acid sequences of a-factor mating peptides from Saccharomyces cerevisiae."
    Betz R., Crabb J.W., Meyer H.E., Wittig R., Duntze W.
    J. Biol. Chem. 262:546-548(1987) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 24-35.
  10. "Structure of Saccharomyces cerevisiae mating hormone a-factor. Identification of S-farnesyl cysteine as a structural component."
    Anderegg R.J., Betz R., Carr S.A., Crabb J.W., Duntze W.
    J. Biol. Chem. 263:18236-18240(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: ISOPRENYLATION AT CYS-35, METHYLATION AT CYS-35.

Entry informationi

Entry nameiMFA2_YEAST
AccessioniPrimary (citable) accession number: P34166
Secondary accession number(s): D6W138
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: February 1, 1994
Last modified: October 29, 2014
This is version 113 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  2. Yeast chromosome XIV
    Yeast (Saccharomyces cerevisiae) chromosome XIV: entries and gene names

External Data

Dasty 3