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P34166 (MFA2_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 111. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Mating hormone A-factor 2
Gene names
Name:MFA2
Ordered Locus Names:YNL145W
ORF Names:N1204, N1787
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length38 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

The active factor is excreted into the culture medium by haploid cells of the A mating type and acts on cells of the opposite mating type (type alpha). It mediates the conjugation process between the two types by inhibiting the initiation of DNA synthesis in type alpha cells and synchronizing them with type A.

Subcellular location

Cell membrane; Lipid-anchor; Cytoplasmic side Potential.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Propeptide1 – 2323
PRO_0000021693
Peptide24 – 3512Mating hormone A-factor 2 Ref.9
PRO_0000021694
Propeptide36 – 383Removed in mature form
PRO_0000021695

Amino acid modifications

Modified residue351Cysteine methyl ester Ref.10
Lipidation351S-farnesyl cysteine Ref.10

Sequences

Sequence LengthMass (Da)Tools
P34166 [UniParc].

Last modified February 1, 1994. Version 1.
Checksum: C71466AEA2F133C4

FASTA384,229
        10         20         30 
MQPITTASTQ ATQKDKSSEK KDNYIIKGLF WDPACVIA 

« Hide

References

« Hide 'large scale' references
[1]"Structure of genes encoding precursors of the yeast peptide mating pheromone a-factor."
Brake A.J., Brenner C., Najarian R., Laybourn P., Merryweather J.
(In) Gething M.-J. (eds.); Protein transport and secretion, pp.103-108, Cold Spring Harbor Laboratory Press, New York (1985)
Cited for: NUCLEOTIDE SEQUENCE.
[2]"The a-factor pheromone of Saccharomyces cerevisiae is essential for mating."
Michaelis S., Herskowitz I.
Mol. Cell. Biol. 8:1309-1318(1988) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE.
[3]"3'-UTR-dependent deadenylation by the yeast poly(A) nuclease."
Lowell J.E., Rudner D.Z., Sachs A.B.
Genes Dev. 6:2088-2099(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[4]"The sequence of 36.8 kb from the left arm of chromosome XIV reveals 24 complete open reading frames: 18 correspond to new genes, one of which encodes a protein similar to the human myotonic dystrophy kinase."
Nasr F., Becam A.-M., Herbert C.J.
Yeast 12:169-175(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE.
Strain: ATCC 96604 / S288c / FY1679.
[5]"A 43.5 kb segment of yeast chromosome XIV, which contains MFA2, MEP2, CAP/SRV2, NAM9, FKB1/FPR1/RBP1, MOM22 and CPT1, predicts an adenosine deaminase gene and 14 new open reading frames."
Mallet L., Bussereau F., Jacquet M.
Yeast 11:1195-1209(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[6]"The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV and its evolutionary implications."
Philippsen P., Kleine K., Poehlmann R., Duesterhoeft A., Hamberg K., Hegemann J.H., Obermaier B., Urrestarazu L.A., Aert R., Albermann K., Altmann R., Andre B., Baladron V., Ballesta J.P.G., Becam A.-M., Beinhauer J.D., Boskovic J., Buitrago M.J. expand/collapse author list , Bussereau F., Coster F., Crouzet M., D'Angelo M., Dal Pero F., De Antoni A., del Rey F., Doignon F., Domdey H., Dubois E., Fiedler T.A., Fleig U., Floeth M., Fritz C., Gaillardin C., Garcia-Cantalejo J.M., Glansdorff N., Goffeau A., Gueldener U., Herbert C.J., Heumann K., Heuss-Neitzel D., Hilbert H., Hinni K., Iraqui Houssaini I., Jacquet M., Jimenez A., Jonniaux J.-L., Karpfinger-Hartl L., Lanfranchi G., Lepingle A., Levesque H., Lyck R., Maftahi M., Mallet L., Maurer C.T.C., Messenguy F., Mewes H.-W., Moestl D., Nasr F., Nicaud J.-M., Niedenthal R.K., Pandolfo D., Pierard A., Piravandi E., Planta R.J., Pohl T.M., Purnelle B., Rebischung C., Remacha M.A., Revuelta J.L., Rinke M., Saiz J.E., Sartorello F., Scherens B., Sen-Gupta M., Soler-Mira A., Urbanus J.H.M., Valle G., Van Dyck L., Verhasselt P., Vierendeels F., Vissers S., Voet M., Volckaert G., Wach A., Wambutt R., Wedler H., Zollner A., Hani J.
Nature 387:93-98(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[7]"The reference genome sequence of Saccharomyces cerevisiae: Then and now."
Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R., Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S., Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.
G3 (Bethesda) 4:389-398(2014) [PubMed] [Europe PMC] [Abstract]
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[8]"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae."
Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. expand/collapse author list , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J.
Genome Res. 17:536-543(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[9]"Amino acid sequences of a-factor mating peptides from Saccharomyces cerevisiae."
Betz R., Crabb J.W., Meyer H.E., Wittig R., Duntze W.
J. Biol. Chem. 262:546-548(1987) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 24-35.
[10]"Structure of Saccharomyces cerevisiae mating hormone a-factor. Identification of S-farnesyl cysteine as a structural component."
Anderegg R.J., Betz R., Carr S.A., Crabb J.W., Duntze W.
J. Biol. Chem. 263:18236-18240(1988) [PubMed] [Europe PMC] [Abstract]
Cited for: ISOPRENYLATION AT CYS-35, METHYLATION AT CYS-35.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U20823 Genomic DNA. Translation: AAA68602.1.
U26204 Genomic DNA. Translation: AAA67688.1.
Z46843 Genomic DNA. Translation: CAA86881.1.
S48033 mRNA. Translation: AAD13854.1.
Z71421 Genomic DNA. Translation: CAA96028.1.
AY693189 Genomic DNA. Translation: AAT93208.1.
BK006947 Genomic DNA. Translation: DAA10404.1.
PIRS55139.
RefSeqNP_014254.1. NM_001182983.1.

3D structure databases

ProteinModelPortalP34166.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid35683. 63 interactions.
DIPDIP-2140N.
IntActP34166. 1 interaction.
MINTMINT-527449.
STRING4932.YNL145W.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYNL145W; YNL145W; YNL145W.
GeneID855577.
KEGGsce:YNL145W.

Organism-specific databases

CYGDYNL145w.
SGDS000005089. MFA2.

Phylogenomic databases

KOK11235.
OrthoDBEOG7T1RR3.

Enzyme and pathway databases

BioCycYEAST:G3O-33163-MONOMER.

Gene expression databases

GenevestigatorP34166.

Family and domain databases

ProtoNetSearch...

Other

NextBio979700.

Entry information

Entry nameMFA2_YEAST
AccessionPrimary (citable) accession number: P34166
Secondary accession number(s): D6W138
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: February 1, 1994
Last modified: July 9, 2014
This is version 111 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome XIV

Yeast (Saccharomyces cerevisiae) chromosome XIV: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD