P34166 (MFA2_YEAST) Reviewed, UniProtKB/Swiss-Prot
Last modified
May 29, 2013.
Version 104.
History...
Clusters with 
Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize orderNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize orderNames and origin
| Protein names | Recommended name: Mating hormone A-factor 2 | ||||||
| Gene names |
| ||||||
| Organism | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome] | ||||||
| Taxonomic identifier | 559292 [NCBI] | ||||||
| Taxonomic lineage | Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces ›  |
Protein attributes
| Sequence length | 38 AA. |
| Sequence status | Complete. |
| Sequence processing | The displayed sequence is further processed into a mature form. |
| Protein existence | Evidence at protein level |
General annotation (Comments)
| Function | The active factor is excreted into the culture medium by haploid cells of the A mating type and acts on cells of the opposite mating type (type alpha). It mediates the conjugation process between the two types by inhibiting the initiation of DNA synthesis in type alpha cells and synchronizing them with type A. |
| Subcellular location | Cell membrane; Lipid-anchor; Cytoplasmic side Potential. |
Ontologies
| Keywords | |
|---|---|
| Cellular component | Cell membrane Membrane |
| Molecular function | Pheromone |
| PTM | Lipoprotein Methylation Prenylation |
| Technical term | Complete proteome Direct protein sequencing Reference proteome |
| Gene Ontology (GO) | |
| Biological_process | pheromone-dependent signal transduction involved in conjugation with cellular fusion Inferred from genetic interaction Ref.2. Source: SGD |
| Cellular_component | extracellular region Inferred from direct assay PubMed 2046670. Source: SGD plasma membraneInferred from electronic annotation. Source: UniProtKB-SubCell |
| Molecular_function | mating pheromone activity Inferred from mutant phenotype Ref.2. Source: SGD |
| Complete GO annotation... | |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Propeptide | 1 – 23 | 23 | PRO_0000021693 | ||||||
| Peptide | 24 – 35 | 12 | Mating hormone A-factor 2 Ref.9 | PRO_0000021694 | |||||
| Propeptide | 36 – 38 | 3 | Removed in mature form | PRO_0000021695 | |||||
Amino acid modifications | |||||||||
| Modified residue | 35 | 1 | Cysteine methyl ester Ref.10 | ||||||
| Lipidation | 35 | 1 | S-farnesyl cysteine Ref.10 | ||||||
Sequences
References
| « Hide 'large scale' references | |
| [1] | "Structure of genes encoding precursors of the yeast peptide mating pheromone a-factor." Brake A.J., Brenner C., Najarian R., Laybourn P., Merryweather J. (In) Gething M.-J. (eds.); Protein transport and secretion, pp.103-108, Cold Spring Harbor Laboratory Press, New York (1985) Cited for: NUCLEOTIDE SEQUENCE. |
| [2] | "The a-factor pheromone of Saccharomyces cerevisiae is essential for mating." Michaelis S., Herskowitz I. Mol. Cell. Biol. 8:1309-1318(1988) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE. |
| [3] | "3'-UTR-dependent deadenylation by the yeast poly(A) nuclease." Lowell J.E., Rudner D.Z., Sachs A.B. Genes Dev. 6:2088-2099(1992) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. |
| [4] | "The sequence of 36.8 kb from the left arm of chromosome XIV reveals 24 complete open reading frames: 18 correspond to new genes, one of which encodes a protein similar to the human myotonic dystrophy kinase." Nasr F., Becam A.-M., Herbert C.J. Yeast 12:169-175(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE. Strain: ATCC 96604 / S288c / FY1679. |
| [5] | "A 43.5 kb segment of yeast chromosome XIV, which contains MFA2, MEP2, CAP/SRV2, NAM9, FKB1/FPR1/RBP1, MOM22 and CPT1, predicts an adenosine deaminase gene and 14 new open reading frames." Mallet L., Bussereau F., Jacquet M. Yeast 11:1195-1209(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c. |
| [6] | "The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV and its evolutionary implications." Philippsen P., Kleine K., Poehlmann R., Duesterhoeft A., Hamberg K., Hegemann J.H., Obermaier B., Urrestarazu L.A., Aert R., Albermann K., Altmann R., Andre B., Baladron V., Ballesta J.P.G., Becam A.-M., Beinhauer J.D., Boskovic J., Buitrago M.J.  Hani J.Nature 387:93-98(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679. |
| [7] | Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c. |
| [8] | "Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae." Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. LaBaer J.Genome Res. 17:536-543(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c. |
| [9] | "Amino acid sequences of a-factor mating peptides from Saccharomyces cerevisiae." Betz R., Crabb J.W., Meyer H.E., Wittig R., Duntze W. J. Biol. Chem. 262:546-548(1987) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 24-35. |
| [10] | "Structure of Saccharomyces cerevisiae mating hormone a-factor. Identification of S-farnesyl cysteine as a structural component." Anderegg R.J., Betz R., Carr S.A., Crabb J.W., Duntze W. J. Biol. Chem. 263:18236-18240(1988) [PubMed] [Europe PMC] [Abstract] Cited for: ISOPRENYLATION AT CYS-35, METHYLATION AT CYS-35. |
| + | Additional computationally mapped references. |
Cross-references
Sequence databases | |
|---|---|
| EMBL GenBank DDBJ | U20823 Genomic DNA. Translation: AAA68602.1. U26204 Genomic DNA. Translation: AAA67688.1. Z46843 Genomic DNA. Translation: CAA86881.1. S48033 mRNA. Translation: AAD13854.1. Z71421 Genomic DNA. Translation: CAA96028.1. AY693189 Genomic DNA. Translation: AAT93208.1. BK006947 Genomic DNA. Translation: DAA10404.1. |
| PIR | S55139. |
| RefSeq | NP_014254.1. NM_001182983.1. |
3D structure databases | |
| ProteinModelPortal | P34166. |
| ModBase | Search... |
Protein-protein interaction databases | |
| DIP | DIP-2140N. |
| IntAct | P34166. 1 interaction. |
| MINT | MINT-527449. |
| STRING | 4932.YNL145W. |
Protocols and materials databases | |
| StructuralBiologyKnowledgebase | Search... |
Genome annotation databases | |
| EnsemblFungi | YNL145W; YNL145W; YNL145W. |
| GeneID | 855577. |
| KEGG | sce:YNL145W. |
Organism-specific databases | |
| CYGD | YNL145w. |
| SGD | S000005089. MFA2. |
Phylogenomic databases | |
| GeneTree | ENSGT00390000009247. |
| KO | K11235. |
| OrthoDB | EOG4SXRQ0. |
Enzyme and pathway databases | |
| BioCyc | YEAST:G3O-33163-MONOMER. |
Gene expression databases | |
| Genevestigator | P34166. |
| GermOnline | YNL145W. Saccharomyces cerevisiae. |
Family and domain databases | |
| ProtoNet | Search... |
Other | |
| NextBio | 979700. |
Entry information
| Entry name | MFA2_YEAST | ||||||||
| Accession | Primary (citable) accession number: P34166 Secondary accession number(s): D6W138 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation program | Fungal Protein Annotation Program | ||||||||
Relevant documents
| Yeast Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD |
| Yeast chromosome XIV Yeast (Saccharomyces cerevisiae) chromosome XIV: entries and gene names |