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P34165 (MFA1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 110. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Mating hormone A-factor 1
Gene names
Name:MFA1
Ordered Locus Names:YDR461W
ORF Names:D8035.5
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length36 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

The active factor is excreted into the culture medium by haploid cells of the A mating type and acts on cells of the opposite mating type (type alpha). It mediates the conjugation process between the two types by inhibiting the initiation of DNA synthesis in type alpha cells and synchronizing them with type A.

Subcellular location

Cell membrane; Lipid-anchor; Cytoplasmic side Potential.

Miscellaneous

Present with 28900 molecules/cell in log phase SD medium.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Propeptide1 – 2121
PRO_0000021690
Peptide22 – 3312Mating hormone A-factor 1 Ref.6
PRO_0000021691
Propeptide34 – 363Removed in mature form
PRO_0000021692

Amino acid modifications

Modified residue331Cysteine methyl ester
Lipidation331S-farnesyl cysteine Ref.7

Sequences

Sequence LengthMass (Da)Tools
P34165 [UniParc].

Last modified February 1, 1994. Version 1.
Checksum: 03E051D7D6C2522F

FASTA363,927
        10         20         30 
MQPSTATAAP KEKTSSEKKD NYIIKGVFWD PACVIA 

« Hide

References

« Hide 'large scale' references
[1]"Structure of genes encoding precursors of the yeast peptide mating pheromone a-factor."
Brake A.J., Brenner C., Najarian R., Laybourn P., Merryweather J.
(In) Gething M.-J. (eds.); Protein transport and secretion, pp.103-108, Cold Spring Harbor Laboratory Press, New York (1985)
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"The a-factor pheromone of Saccharomyces cerevisiae is essential for mating."
Michaelis S., Herskowitz I.
Mol. Cell. Biol. 8:1309-1318(1988) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]"The nucleotide sequence of Saccharomyces cerevisiae chromosome IV."
Jacq C., Alt-Moerbe J., Andre B., Arnold W., Bahr A., Ballesta J.P.G., Bargues M., Baron L., Becker A., Biteau N., Bloecker H., Blugeon C., Boskovic J., Brandt P., Brueckner M., Buitrago M.J., Coster F., Delaveau T. expand/collapse author list , del Rey F., Dujon B., Eide L.G., Garcia-Cantalejo J.M., Goffeau A., Gomez-Peris A., Granotier C., Hanemann V., Hankeln T., Hoheisel J.D., Jaeger W., Jimenez A., Jonniaux J.-L., Kraemer C., Kuester H., Laamanen P., Legros Y., Louis E.J., Moeller-Rieker S., Monnet A., Moro M., Mueller-Auer S., Nussbaumer B., Paricio N., Paulin L., Perea J., Perez-Alonso M., Perez-Ortin J.E., Pohl T.M., Prydz H., Purnelle B., Rasmussen S.W., Remacha M.A., Revuelta J.L., Rieger M., Salom D., Saluz H.P., Saiz J.E., Saren A.-M., Schaefer M., Scharfe M., Schmidt E.R., Schneider C., Scholler P., Schwarz S., Soler-Mira A., Urrestarazu L.A., Verhasselt P., Vissers S., Voet M., Volckaert G., Wagner G., Wambutt R., Wedler E., Wedler H., Woelfl S., Harris D.E., Bowman S., Brown D., Churcher C.M., Connor R., Dedman K., Gentles S., Hamlin N., Hunt S., Jones L., McDonald S., Murphy L.D., Niblett D., Odell C., Oliver K., Rajandream M.A., Richards C., Shore L., Walsh S.V., Barrell B.G., Dietrich F.S., Mulligan J.T., Allen E., Araujo R., Aviles E., Berno A., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M., Hunicke-Smith S., Hyman R.W., Komp C., Lashkari D., Lew H., Lin D., Mosedale D., Nakahara K., Namath A., Oefner P., Oh C., Petel F.X., Roberts D., Schramm S., Schroeder M., Shogren T., Shroff N., Winant A., Yelton M.A., Botstein D., Davis R.W., Johnston M., Andrews S., Brinkman R., Cooper J., Ding H., Du Z., Favello A., Fulton L., Gattung S., Greco T., Hallsworth K., Hawkins J., Hillier L.W., Jier M., Johnson D., Johnston L., Kirsten J., Kucaba T., Langston Y., Latreille P., Le T., Mardis E., Menezes S., Miller N., Nhan M., Pauley A., Peluso D., Rifkin L., Riles L., Taich A., Trevaskis E., Vignati D., Wilcox L., Wohldman P., Vaudin M., Wilson R., Waterston R., Albermann K., Hani J., Heumann K., Kleine K., Mewes H.-W., Zollner A., Zaccaria P.
Nature 387:75-78(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[4]Saccharomyces Genome Database
Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[5]"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae."
Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. expand/collapse author list , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J.
Genome Res. 17:536-543(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[6]"Amino acid sequences of a-factor mating peptides from Saccharomyces cerevisiae."
Betz R., Crabb J.W., Meyer H.E., Wittig R., Duntze W.
J. Biol. Chem. 262:546-548(1987) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 22-33.
[7]"Structure of Saccharomyces cerevisiae mating hormone a-factor. Identification of S-farnesyl cysteine as a structural component."
Anderegg R.J., Betz R., Carr S.A., Crabb J.W., Duntze W.
J. Biol. Chem. 263:18236-18240(1988) [PubMed] [Europe PMC] [Abstract]
Cited for: ISOPRENYLATION AT CYS-33, IDENTIFICATION BY MASS SPECTROMETRY.
[8]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[9]"The conformation of a-factor is not influenced by the S-prenylation of Cys12."
Gounarides J.S., Broido M.S., Xue C.-B., Becker J.M., Naider F.R.
Biochem. Biophys. Res. Commun. 181:1125-1130(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: STRUCTURE BY NMR.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U20817 Genomic DNA. Translation: AAA68601.1.
U26203 Genomic DNA. Translation: AAA67687.1.
U33050 Genomic DNA. Translation: AAB64920.1.
AY557797 Genomic DNA. Translation: AAS56123.1.
BK006938 Genomic DNA. Translation: DAA12295.1.
PIRS59735.
RefSeqNP_010749.3. NM_001180769.3.

3D structure databases

ProteinModelPortalP34165.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid32515. 8 interactions.
DIPDIP-4646N.
MINTMINT-567416.
STRING4932.YDR461W.

Proteomic databases

PaxDbP34165.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYDR461W; YDR461W; YDR461W.
GeneID852072.
KEGGsce:YDR461W.

Organism-specific databases

CYGDYDR461w.
SGDS000002869. MFA1.

Phylogenomic databases

KOK11235.
OrthoDBEOG7T1RR3.

Enzyme and pathway databases

BioCycYEAST:G3O-29989-MONOMER.

Gene expression databases

GenevestigatorP34165.

Family and domain databases

ProtoNetSearch...

Other

NextBio970367.
PMAP-CutDBP34165.

Entry information

Entry nameMFA1_YEAST
AccessionPrimary (citable) accession number: P34165
Secondary accession number(s): D6VT85
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: February 1, 1994
Last modified: April 16, 2014
This is version 110 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome IV

Yeast (Saccharomyces cerevisiae) chromosome IV: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD