Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

P34165

- MFA1_YEAST

UniProt

P34165 - MFA1_YEAST

Protein

Mating hormone A-factor 1

Gene

MFA1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
  1. Functioni

    The active factor is excreted into the culture medium by haploid cells of the A mating type and acts on cells of the opposite mating type (type alpha). It mediates the conjugation process between the two types by inhibiting the initiation of DNA synthesis in type alpha cells and synchronizing them with type A.

    GO - Molecular functioni

    1. mating pheromone activity Source: SGD

    GO - Biological processi

    1. pheromone-dependent signal transduction involved in conjugation with cellular fusion Source: SGD

    Keywords - Molecular functioni

    Pheromone

    Enzyme and pathway databases

    BioCyciYEAST:G3O-29989-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Mating hormone A-factor 1
    Gene namesi
    Name:MFA1
    Ordered Locus Names:YDR461W
    ORF Names:D8035.5
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome IV

    Organism-specific databases

    CYGDiYDR461w.
    SGDiS000002869. MFA1.

    Subcellular locationi

    Cell membrane Curated; Lipid-anchor Curated; Cytoplasmic side Curated

    GO - Cellular componenti

    1. extracellular region Source: SGD
    2. plasma membrane Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cell membrane, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Propeptidei1 – 21211 PublicationPRO_0000021690Add
    BLAST
    Peptidei22 – 3312Mating hormone A-factor 1PRO_0000021691Add
    BLAST
    Propeptidei34 – 363Removed in mature formPRO_0000021692

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei33 – 331Cysteine methyl ester
    Lipidationi33 – 331S-farnesyl cysteine1 Publication

    Keywords - PTMi

    Lipoprotein, Methylation, Prenylation

    Proteomic databases

    PaxDbiP34165.

    Miscellaneous databases

    PMAP-CutDBP34165.

    Expressioni

    Gene expression databases

    GenevestigatoriP34165.

    Interactioni

    Protein-protein interaction databases

    BioGridi32515. 9 interactions.
    DIPiDIP-4646N.
    MINTiMINT-567416.
    STRINGi4932.YDR461W.

    Structurei

    3D structure databases

    ProteinModelPortaliP34165.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Phylogenomic databases

    KOiK11235.
    OrthoDBiEOG7T1RR3.

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P34165-1 [UniParc]FASTAAdd to Basket

    « Hide

    MQPSTATAAP KEKTSSEKKD NYIIKGVFWD PACVIA                  36
    Length:36
    Mass (Da):3,927
    Last modified:February 1, 1994 - v1
    Checksum:i03E051D7D6C2522F
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U20817 Genomic DNA. Translation: AAA68601.1.
    U26203 Genomic DNA. Translation: AAA67687.1.
    U33050 Genomic DNA. Translation: AAB64920.1.
    AY557797 Genomic DNA. Translation: AAS56123.1.
    BK006938 Genomic DNA. Translation: DAA12295.1.
    PIRiS59735.
    RefSeqiNP_010749.3. NM_001180769.3.

    Genome annotation databases

    EnsemblFungiiYDR461W; YDR461W; YDR461W.
    GeneIDi852072.
    KEGGisce:YDR461W.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U20817 Genomic DNA. Translation: AAA68601.1 .
    U26203 Genomic DNA. Translation: AAA67687.1 .
    U33050 Genomic DNA. Translation: AAB64920.1 .
    AY557797 Genomic DNA. Translation: AAS56123.1 .
    BK006938 Genomic DNA. Translation: DAA12295.1 .
    PIRi S59735.
    RefSeqi NP_010749.3. NM_001180769.3.

    3D structure databases

    ProteinModelPortali P34165.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 32515. 9 interactions.
    DIPi DIP-4646N.
    MINTi MINT-567416.
    STRINGi 4932.YDR461W.

    Proteomic databases

    PaxDbi P34165.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YDR461W ; YDR461W ; YDR461W .
    GeneIDi 852072.
    KEGGi sce:YDR461W.

    Organism-specific databases

    CYGDi YDR461w.
    SGDi S000002869. MFA1.

    Phylogenomic databases

    KOi K11235.
    OrthoDBi EOG7T1RR3.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-29989-MONOMER.

    Miscellaneous databases

    NextBioi 970367.
    PMAP-CutDB P34165.

    Gene expression databases

    Genevestigatori P34165.

    Family and domain databases

    ProtoNeti Search...

    Publicationsi

    1. "Structure of genes encoding precursors of the yeast peptide mating pheromone a-factor."
      Brake A.J., Brenner C., Najarian R., Laybourn P., Merryweather J.
      (In) Gething M.-J. (eds.); Protein transport and secretion, pp.103-108, Cold Spring Harbor Laboratory Press, New York (1985)
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. "The a-factor pheromone of Saccharomyces cerevisiae is essential for mating."
      Michaelis S., Herskowitz I.
      Mol. Cell. Biol. 8:1309-1318(1988) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    3. "The nucleotide sequence of Saccharomyces cerevisiae chromosome IV."
      Jacq C., Alt-Moerbe J., Andre B., Arnold W., Bahr A., Ballesta J.P.G., Bargues M., Baron L., Becker A., Biteau N., Bloecker H., Blugeon C., Boskovic J., Brandt P., Brueckner M., Buitrago M.J., Coster F., Delaveau T.
      , del Rey F., Dujon B., Eide L.G., Garcia-Cantalejo J.M., Goffeau A., Gomez-Peris A., Granotier C., Hanemann V., Hankeln T., Hoheisel J.D., Jaeger W., Jimenez A., Jonniaux J.-L., Kraemer C., Kuester H., Laamanen P., Legros Y., Louis E.J., Moeller-Rieker S., Monnet A., Moro M., Mueller-Auer S., Nussbaumer B., Paricio N., Paulin L., Perea J., Perez-Alonso M., Perez-Ortin J.E., Pohl T.M., Prydz H., Purnelle B., Rasmussen S.W., Remacha M.A., Revuelta J.L., Rieger M., Salom D., Saluz H.P., Saiz J.E., Saren A.-M., Schaefer M., Scharfe M., Schmidt E.R., Schneider C., Scholler P., Schwarz S., Soler-Mira A., Urrestarazu L.A., Verhasselt P., Vissers S., Voet M., Volckaert G., Wagner G., Wambutt R., Wedler E., Wedler H., Woelfl S., Harris D.E., Bowman S., Brown D., Churcher C.M., Connor R., Dedman K., Gentles S., Hamlin N., Hunt S., Jones L., McDonald S., Murphy L.D., Niblett D., Odell C., Oliver K., Rajandream M.A., Richards C., Shore L., Walsh S.V., Barrell B.G., Dietrich F.S., Mulligan J.T., Allen E., Araujo R., Aviles E., Berno A., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M., Hunicke-Smith S., Hyman R.W., Komp C., Lashkari D., Lew H., Lin D., Mosedale D., Nakahara K., Namath A., Oefner P., Oh C., Petel F.X., Roberts D., Schramm S., Schroeder M., Shogren T., Shroff N., Winant A., Yelton M.A., Botstein D., Davis R.W., Johnston M., Andrews S., Brinkman R., Cooper J., Ding H., Du Z., Favello A., Fulton L., Gattung S., Greco T., Hallsworth K., Hawkins J., Hillier L.W., Jier M., Johnson D., Johnston L., Kirsten J., Kucaba T., Langston Y., Latreille P., Le T., Mardis E., Menezes S., Miller N., Nhan M., Pauley A., Peluso D., Rifkin L., Riles L., Taich A., Trevaskis E., Vignati D., Wilcox L., Wohldman P., Vaudin M., Wilson R., Waterston R., Albermann K., Hani J., Heumann K., Kleine K., Mewes H.-W., Zollner A., Zaccaria P.
      Nature 387:75-78(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    4. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    5. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    6. "Amino acid sequences of a-factor mating peptides from Saccharomyces cerevisiae."
      Betz R., Crabb J.W., Meyer H.E., Wittig R., Duntze W.
      J. Biol. Chem. 262:546-548(1987) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 22-33.
    7. "Structure of Saccharomyces cerevisiae mating hormone a-factor. Identification of S-farnesyl cysteine as a structural component."
      Anderegg R.J., Betz R., Carr S.A., Crabb J.W., Duntze W.
      J. Biol. Chem. 263:18236-18240(1988) [PubMed] [Europe PMC] [Abstract]
      Cited for: ISOPRENYLATION AT CYS-33, MTEHYLATION AT CYS-33, IDENTIFICATION BY MASS SPECTROMETRY.
    8. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    9. "The conformation of a-factor is not influenced by the S-prenylation of Cys12."
      Gounarides J.S., Broido M.S., Xue C.-B., Becker J.M., Naider F.R.
      Biochem. Biophys. Res. Commun. 181:1125-1130(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: STRUCTURE BY NMR.

    Entry informationi

    Entry nameiMFA1_YEAST
    AccessioniPrimary (citable) accession number: P34165
    Secondary accession number(s): D6VT85
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1994
    Last sequence update: February 1, 1994
    Last modified: October 1, 2014
    This is version 114 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 28900 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    2. Yeast chromosome IV
      Yeast (Saccharomyces cerevisiae) chromosome IV: entries and gene names

    External Data

    Dasty 3