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P34165

- MFA1_YEAST

UniProt

P34165 - MFA1_YEAST

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Protein
Mating hormone A-factor 1
Gene
MFA1, YDR461W, D8035.5
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at protein leveli

Functioni

The active factor is excreted into the culture medium by haploid cells of the A mating type and acts on cells of the opposite mating type (type alpha). It mediates the conjugation process between the two types by inhibiting the initiation of DNA synthesis in type alpha cells and synchronizing them with type A.

GO - Molecular functioni

  1. mating pheromone activity Source: SGD

GO - Biological processi

  1. pheromone-dependent signal transduction involved in conjugation with cellular fusion Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Pheromone

Enzyme and pathway databases

BioCyciYEAST:G3O-29989-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Mating hormone A-factor 1
Gene namesi
Name:MFA1
Ordered Locus Names:YDR461W
ORF Names:D8035.5
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome IV

Organism-specific databases

CYGDiYDR461w.
SGDiS000002869. MFA1.

Subcellular locationi

GO - Cellular componenti

  1. extracellular region Source: SGD
  2. plasma membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Propeptidei1 – 2121
PRO_0000021690Add
BLAST
Peptidei22 – 3312Mating hormone A-factor 11 Publication
PRO_0000021691Add
BLAST
Propeptidei34 – 363Removed in mature form
PRO_0000021692

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei33 – 331Cysteine methyl ester
Lipidationi33 – 331S-farnesyl cysteine1 Publication

Keywords - PTMi

Lipoprotein, Methylation, Prenylation

Proteomic databases

PaxDbiP34165.

Miscellaneous databases

PMAP-CutDBP34165.

Expressioni

Gene expression databases

GenevestigatoriP34165.

Interactioni

Protein-protein interaction databases

BioGridi32515. 9 interactions.
DIPiDIP-4646N.
MINTiMINT-567416.
STRINGi4932.YDR461W.

Structurei

3D structure databases

ProteinModelPortaliP34165.

Family & Domainsi

Phylogenomic databases

KOiK11235.
OrthoDBiEOG7T1RR3.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P34165-1 [UniParc]FASTAAdd to Basket

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MQPSTATAAP KEKTSSEKKD NYIIKGVFWD PACVIA                  36
Length:36
Mass (Da):3,927
Last modified:February 1, 1994 - v1
Checksum:i03E051D7D6C2522F
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U20817 Genomic DNA. Translation: AAA68601.1.
U26203 Genomic DNA. Translation: AAA67687.1.
U33050 Genomic DNA. Translation: AAB64920.1.
AY557797 Genomic DNA. Translation: AAS56123.1.
BK006938 Genomic DNA. Translation: DAA12295.1.
PIRiS59735.
RefSeqiNP_010749.3. NM_001180769.3.

Genome annotation databases

EnsemblFungiiYDR461W; YDR461W; YDR461W.
GeneIDi852072.
KEGGisce:YDR461W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U20817 Genomic DNA. Translation: AAA68601.1 .
U26203 Genomic DNA. Translation: AAA67687.1 .
U33050 Genomic DNA. Translation: AAB64920.1 .
AY557797 Genomic DNA. Translation: AAS56123.1 .
BK006938 Genomic DNA. Translation: DAA12295.1 .
PIRi S59735.
RefSeqi NP_010749.3. NM_001180769.3.

3D structure databases

ProteinModelPortali P34165.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 32515. 9 interactions.
DIPi DIP-4646N.
MINTi MINT-567416.
STRINGi 4932.YDR461W.

Proteomic databases

PaxDbi P34165.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YDR461W ; YDR461W ; YDR461W .
GeneIDi 852072.
KEGGi sce:YDR461W.

Organism-specific databases

CYGDi YDR461w.
SGDi S000002869. MFA1.

Phylogenomic databases

KOi K11235.
OrthoDBi EOG7T1RR3.

Enzyme and pathway databases

BioCyci YEAST:G3O-29989-MONOMER.

Miscellaneous databases

NextBioi 970367.
PMAP-CutDB P34165.

Gene expression databases

Genevestigatori P34165.

Family and domain databases

ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Structure of genes encoding precursors of the yeast peptide mating pheromone a-factor."
    Brake A.J., Brenner C., Najarian R., Laybourn P., Merryweather J.
    (In) Gething M.-J. (eds.); Protein transport and secretion, pp.103-108, Cold Spring Harbor Laboratory Press, New York (1985)
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "The a-factor pheromone of Saccharomyces cerevisiae is essential for mating."
    Michaelis S., Herskowitz I.
    Mol. Cell. Biol. 8:1309-1318(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  3. "The nucleotide sequence of Saccharomyces cerevisiae chromosome IV."
    Jacq C., Alt-Moerbe J., Andre B., Arnold W., Bahr A., Ballesta J.P.G., Bargues M., Baron L., Becker A., Biteau N., Bloecker H., Blugeon C., Boskovic J., Brandt P., Brueckner M., Buitrago M.J., Coster F., Delaveau T.
    , del Rey F., Dujon B., Eide L.G., Garcia-Cantalejo J.M., Goffeau A., Gomez-Peris A., Granotier C., Hanemann V., Hankeln T., Hoheisel J.D., Jaeger W., Jimenez A., Jonniaux J.-L., Kraemer C., Kuester H., Laamanen P., Legros Y., Louis E.J., Moeller-Rieker S., Monnet A., Moro M., Mueller-Auer S., Nussbaumer B., Paricio N., Paulin L., Perea J., Perez-Alonso M., Perez-Ortin J.E., Pohl T.M., Prydz H., Purnelle B., Rasmussen S.W., Remacha M.A., Revuelta J.L., Rieger M., Salom D., Saluz H.P., Saiz J.E., Saren A.-M., Schaefer M., Scharfe M., Schmidt E.R., Schneider C., Scholler P., Schwarz S., Soler-Mira A., Urrestarazu L.A., Verhasselt P., Vissers S., Voet M., Volckaert G., Wagner G., Wambutt R., Wedler E., Wedler H., Woelfl S., Harris D.E., Bowman S., Brown D., Churcher C.M., Connor R., Dedman K., Gentles S., Hamlin N., Hunt S., Jones L., McDonald S., Murphy L.D., Niblett D., Odell C., Oliver K., Rajandream M.A., Richards C., Shore L., Walsh S.V., Barrell B.G., Dietrich F.S., Mulligan J.T., Allen E., Araujo R., Aviles E., Berno A., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M., Hunicke-Smith S., Hyman R.W., Komp C., Lashkari D., Lew H., Lin D., Mosedale D., Nakahara K., Namath A., Oefner P., Oh C., Petel F.X., Roberts D., Schramm S., Schroeder M., Shogren T., Shroff N., Winant A., Yelton M.A., Botstein D., Davis R.W., Johnston M., Andrews S., Brinkman R., Cooper J., Ding H., Du Z., Favello A., Fulton L., Gattung S., Greco T., Hallsworth K., Hawkins J., Hillier L.W., Jier M., Johnson D., Johnston L., Kirsten J., Kucaba T., Langston Y., Latreille P., Le T., Mardis E., Menezes S., Miller N., Nhan M., Pauley A., Peluso D., Rifkin L., Riles L., Taich A., Trevaskis E., Vignati D., Wilcox L., Wohldman P., Vaudin M., Wilson R., Waterston R., Albermann K., Hani J., Heumann K., Kleine K., Mewes H.-W., Zollner A., Zaccaria P.
    Nature 387:75-78(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  4. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  5. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  6. "Amino acid sequences of a-factor mating peptides from Saccharomyces cerevisiae."
    Betz R., Crabb J.W., Meyer H.E., Wittig R., Duntze W.
    J. Biol. Chem. 262:546-548(1987) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 22-33.
  7. "Structure of Saccharomyces cerevisiae mating hormone a-factor. Identification of S-farnesyl cysteine as a structural component."
    Anderegg R.J., Betz R., Carr S.A., Crabb J.W., Duntze W.
    J. Biol. Chem. 263:18236-18240(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: ISOPRENYLATION AT CYS-33, MTEHYLATION AT CYS-33, IDENTIFICATION BY MASS SPECTROMETRY.
  8. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  9. "The conformation of a-factor is not influenced by the S-prenylation of Cys12."
    Gounarides J.S., Broido M.S., Xue C.-B., Becker J.M., Naider F.R.
    Biochem. Biophys. Res. Commun. 181:1125-1130(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: STRUCTURE BY NMR.

Entry informationi

Entry nameiMFA1_YEAST
AccessioniPrimary (citable) accession number: P34165
Secondary accession number(s): D6VT85
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: February 1, 1994
Last modified: July 9, 2014
This is version 113 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 28900 molecules/cell in log phase SD medium.

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  2. Yeast chromosome IV
    Yeast (Saccharomyces cerevisiae) chromosome IV: entries and gene names

External Data

Dasty 3

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