P34165 (MFA1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Mating hormone A-factor 1

Gene names

Name:	MFA1
Ordered Locus Names:	YDR461W
ORF Names:	D8035.5

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › Saccharomyces cerevisiae

Protein attributes

Sequence length	36 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	The active factor is excreted into the culture medium by haploid cells of the A mating type and acts on cells of the opposite mating type (type alpha). It mediates the conjugation process between the two types by inhibiting the initiation of DNA synthesis in type alpha cells and synchronizing them with type A.
Subcellular location	Cell membrane; Lipid-anchor; Cytoplasmic side Potential.
Miscellaneous	Present with 28900 molecules/cell in log phase SD medium.

Ontologies

Keywords
Cellular component	Cell membrane Membrane
Molecular function	Pheromone
PTM	Lipoprotein Methylation Prenylation
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	pheromone-dependent signal transduction involved in conjugation with cellular fusion Inferred from mutant phenotype Ref.2. Source: SGD
Cellular_component	extracellular region Inferred from direct assay PubMed 10022942. Source: SGD plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	mating pheromone activity Inferred from mutant phenotype Ref.2. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Propeptide	1 – 21	21			PRO_0000021690
	Peptide	22 – 33	12	Mating hormone A-factor 1 Ref.6		PRO_0000021691
	Propeptide	34 – 36	3	Removed in mature form		PRO_0000021692
Amino acid modifications
	Modified residue	33	1	Cysteine methyl ester
	Lipidation	33	1	S-farnesyl cysteine Ref.7

Sequences

	Sequence		Length	Mass (Da)
	P34165 [UniParc]. Last modified February 1, 1994. Version 1. Checksum: 03E051D7D6C2522F	FASTA	36	3,927
	10 20 30 MQPSTATAAP KEKTSSEKKD NYIIKGVFWD PACVIA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Structure of genes encoding precursors of the yeast peptide mating pheromone a-factor." Brake A.J., Brenner C., Najarian R., Laybourn P., Merryweather J. (In) Gething M.-J. (eds.); Protein transport and secretion, pp.103-108, Cold Spring Harbor Laboratory Press, New York (1985) Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"The a-factor pheromone of Saccharomyces cerevisiae is essential for mating." Michaelis S., Herskowitz I. Mol. Cell. Biol. 8:1309-1318(1988) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome IV." Jacq C., Alt-Moerbe J., Andre B., Arnold W., Bahr A., Ballesta J.P.G., Bargues M., Baron L., Becker A., Biteau N., Bloecker H., Blugeon C., Boskovic J., Brandt P., Brueckner M., Buitrago M.J., Coster F., Delaveau T. , del Rey F., Dujon B., Eide L.G., Garcia-Cantalejo J.M., Goffeau A., Gomez-Peris A., Granotier C., Hanemann V., Hankeln T., Hoheisel J.D., Jaeger W., Jimenez A., Jonniaux J.-L., Kraemer C., Kuester H., Laamanen P., Legros Y., Louis E.J., Moeller-Rieker S., Monnet A., Moro M., Mueller-Auer S., Nussbaumer B., Paricio N., Paulin L., Perea J., Perez-Alonso M., Perez-Ortin J.E., Pohl T.M., Prydz H., Purnelle B., Rasmussen S.W., Remacha M.A., Revuelta J.L., Rieger M., Salom D., Saluz H.P., Saiz J.E., Saren A.-M., Schaefer M., Scharfe M., Schmidt E.R., Schneider C., Scholler P., Schwarz S., Soler-Mira A., Urrestarazu L.A., Verhasselt P., Vissers S., Voet M., Volckaert G., Wagner G., Wambutt R., Wedler E., Wedler H., Woelfl S., Harris D.E., Bowman S., Brown D., Churcher C.M., Connor R., Dedman K., Gentles S., Hamlin N., Hunt S., Jones L., McDonald S., Murphy L.D., Niblett D., Odell C., Oliver K., Rajandream M.A., Richards C., Shore L., Walsh S.V., Barrell B.G., Dietrich F.S., Mulligan J.T., Allen E., Araujo R., Aviles E., Berno A., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M., Hunicke-Smith S., Hyman R.W., Komp C., Lashkari D., Lew H., Lin D., Mosedale D., Nakahara K., Namath A., Oefner P., Oh C., Petel F.X., Roberts D., Schramm S., Schroeder M., Shogren T., Shroff N., Winant A., Yelton M.A., Botstein D., Davis R.W., Johnston M., Andrews S., Brinkman R., Cooper J., Ding H., Du Z., Favello A., Fulton L., Gattung S., Greco T., Hallsworth K., Hawkins J., Hillier L.W., Jier M., Johnson D., Johnston L., Kirsten J., Kucaba T., Langston Y., Latreille P., Le T., Mardis E., Menezes S., Miller N., Nhan M., Pauley A., Peluso D., Rifkin L., Riles L., Taich A., Trevaskis E., Vignati D., Wilcox L., Wohldman P., Vaudin M., Wilson R., Waterston R., Albermann K., Hani J., Heumann K., Kleine K., Mewes H.-W., Zollner A., Zaccaria P. Nature 387:75-78(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[4]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[5]	"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae." Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J. Genome Res. 17:536-543(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[6]	"Amino acid sequences of a-factor mating peptides from Saccharomyces cerevisiae." Betz R., Crabb J.W., Meyer H.E., Wittig R., Duntze W. J. Biol. Chem. 262:546-548(1987) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 22-33.
[7]	"Structure of Saccharomyces cerevisiae mating hormone a-factor. Identification of S-farnesyl cysteine as a structural component." Anderegg R.J., Betz R., Carr S.A., Crabb J.W., Duntze W. J. Biol. Chem. 263:18236-18240(1988) [PubMed] [Europe PMC] [Abstract] Cited for: ISOPRENYLATION AT CYS-33, MASS SPECTROMETRY.
[8]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[9]	"The conformation of a-factor is not influenced by the S-prenylation of Cys12." Gounarides J.S., Broido M.S., Xue C.-B., Becker J.M., Naider F.R. Biochem. Biophys. Res. Commun. 181:1125-1130(1991) [PubMed] [Europe PMC] [Abstract] Cited for: STRUCTURE BY NMR.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U20817 Genomic DNA. Translation: AAA68601.1. U26203 Genomic DNA. Translation: AAA67687.1. U33050 Genomic DNA. Translation: AAB64920.1. AY557797 Genomic DNA. Translation: AAS56123.1. BK006938 Genomic DNA. Translation: DAA12295.1.
PIR	S59735.
RefSeq	NP_010749.3. NM_001180769.3.
3D structure databases
ProteinModelPortal	P34165.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-4646N.
MINT	MINT-567416.
STRING	4932.YDR461W.
Proteomic databases
PaxDb	P34165.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YDR461W; YDR461W; YDR461W.
GeneID	852072.
KEGG	sce:YDR461W. sce:YDR464W.
Organism-specific databases
CYGD	YDR461w.
SGD	S000002869. MFA1.
Phylogenomic databases
GeneTree	ENSGT00390000009247.
KO	K11235.
OrthoDB	EOG4SXRQ0.
Gene expression databases
Genevestigator	P34165.
GermOnline	YDR461W. Saccharomyces cerevisiae.
Family and domain databases
ProtoNet	Search...
Other
NextBio	970367.
PMAP-CutDB	P34165.

Entry information

Entry name

MFA1_YEAST

Accession

Primary (citable) accession number: P34165
Secondary accession number(s): D6VT85

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1994
Last sequence update:	February 1, 1994
Last modified:	May 1, 2013
This is version 105 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program