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Protein

SNF1 protein kinase subunit beta-2

Gene

SIP2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Beta subunit of the SNF1 kinase complex, which is required for transcriptional, metabolic, and developmental adaptations in response to glucose limitation. Has a structural role, mediating heterotrimer formation, and a regulatory role, defining carbon source-regulated subcellular location and substrate specificity of the SNF1 kinase complex. Involved in the regulation of aging. Acts as a negative regulator of nuclear SNF1 activity in young cells by sequestering its activating gamma subunit at the plasma membrane.2 Publications

GO - Biological processi

  • cellular response to glucose starvation Source: SGD
  • invasive growth in response to glucose limitation Source: SGD
  • protein phosphorylation Source: SGD
  • regulation of protein complex assembly Source: SGD
  • replicative cell aging Source: SGD
  • signal transduction Source: SGD
Complete GO annotation...

Enzyme and pathway databases

BioCyciYEAST:G3O-30685-MONOMER.
ReactomeiR-SCE-163680. AMPK inhibits chREBP transcriptional activation activity.
R-SCE-200425. Import of palmitoyl-CoA into the mitochondrial matrix.
R-SCE-380972. Energy dependent regulation of mTOR by LKB1-AMPK.

Protein family/group databases

CAZyiCBM48. Carbohydrate-Binding Module Family 48.

Names & Taxonomyi

Protein namesi
Recommended name:
SNF1 protein kinase subunit beta-2
Alternative name(s):
Protein SPM2
SNF1-interacting protein 2
Gene namesi
Name:SIP2
Synonyms:SPM2
Ordered Locus Names:YGL208W
ORF Names:G1155
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome VII

Organism-specific databases

EuPathDBiFungiDB:YGL208W.
SGDiS000003176. SIP2.

Subcellular locationi

  • Cytoplasm 1 Publication
  • Cell membrane 1 Publication; Peripheral membrane protein 1 Publication; Cytoplasmic side 1 Publication

  • Note: Resides in the cytosol during growth in glucose. Excluded from the nucleus. There is an age-associated shift in localization from the plasma membrane to the cytoplasm.1 Publication

GO - Cellular componenti

  • cytoplasm Source: SGD
  • nucleotide-activated protein kinase complex Source: SGD
  • plasma membrane Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Cytoplasm, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi2 – 21G → A: Changes protein distribution from the plasma membrane to the cytoplasm and nucleus and alters the cellular life span. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemoved
Chaini2 – 415414SNF1 protein kinase subunit beta-2PRO_0000204375Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Lipidationi2 – 21N-myristoyl glycine1 Publication
Modified residuei66 – 661PhosphoserineCombined sources
Modified residuei298 – 2981PhosphoserineCombined sources

Post-translational modificationi

Phosphorylated by SNF1 in vitro.1 Publication

Keywords - PTMi

Lipoprotein, Myristate, Phosphoprotein

Proteomic databases

MaxQBiP34164.

PTM databases

iPTMnetiP34164.

Expressioni

Inductioni

Induced upon shift to nonfermentable carbon sources.1 Publication

Interactioni

Subunit structurei

Component of the SNF1 kinase complex, a heterotrimeric complex composed of the catalytic alpha subunit SNF1, one of the three related beta subunits SIP1, SIP2 or GAL83, and the regulatory gamma subunit SNF4. The beta subunit serves as a bridge between the catalytic and the regulatory subunit. Interacts (via KIS domain) with SNF1. Interacts (via ASC domain) with SNF4.5 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
SNF1P067829EBI-17187,EBI-17516
SNF4P129046EBI-17187,EBI-17537

Protein-protein interaction databases

BioGridi33048. 42 interactions.
DIPiDIP-865N.
IntActiP34164. 16 interactions.
MINTiMINT-404843.

Structurei

Secondary structure

1
415
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi164 – 1707Combined sources
Beta strandi177 – 1815Combined sources
Helixi182 – 1843Combined sources
Beta strandi196 – 1983Combined sources
Beta strandi202 – 2087Combined sources
Beta strandi210 – 21910Combined sources
Beta strandi222 – 2243Combined sources
Beta strandi231 – 2333Combined sources
Beta strandi240 – 2456Combined sources
Beta strandi305 – 3073Combined sources
Helixi311 – 3133Combined sources
Helixi316 – 3249Combined sources
Helixi336 – 3383Combined sources
Helixi345 – 3473Combined sources
Helixi349 – 36517Combined sources
Helixi375 – 3773Combined sources
Beta strandi380 – 3834Combined sources
Beta strandi390 – 39910Combined sources
Beta strandi402 – 41110Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2QLVX-ray2.60B/E161-412[»]
3T4NX-ray2.30B304-415[»]
3TDHX-ray2.30B304-415[»]
3TE5X-ray2.50B304-415[»]
ProteinModelPortaliP34164.
SMRiP34164. Positions 161-413.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP34164.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni154 – 335182Kinase-interacting sequence (KIS); required for interaction with SNF1Add
BLAST
Regioni336 – 41580Association with SNF1 kinase complex (ASC) domain; required for interaction with SNF4Add
BLAST

Sequence similaritiesi

Phylogenomic databases

GeneTreeiENSGT00390000001416.
HOGENOMiHOG000093748.
InParanoidiP34164.
OrthoDBiEOG092C4NLT.

Family and domain databases

InterProiIPR032640. AMPK1_CBM.
IPR006828. ASC_dom.
IPR014756. Ig_E-set.
[Graphical view]
PfamiPF16561. AMPK1_CBM. 1 hit.
PF04739. AMPKBI. 1 hit.
[Graphical view]
SMARTiSM01010. AMPKBI. 1 hit.
[Graphical view]
SUPFAMiSSF81296. SSF81296. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P34164-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGTTTSHPAQ KKQTTKKCRA PIMSDVREKP SNAQGCEPQE MDAVSKKVTE
60 70 80 90 100
LSLNKCSDSQ DAGQPSREGS ITKKKSTLLL RDEDEPTMPK LSVMETAVDT
110 120 130 140 150
DSGSSSTSDD EEGDIIAQTT EPKQDASPDD DRSGHSSPRE EGQQQIRAKE
160 170 180 190 200
ASGGPSEIKS SLMVPVEIRW QQGGSKVYVT GSFTKWRKMI GLIPDSDNNG
210 220 230 240 250
SFHVKLRLLP GTHRFRFIVD NELRVSDFLP TATDQMGNFV NYIEVRQPEK
260 270 280 290 300
NPTNEKIRSK EADSMRPPTS DRSSIALQIG KDPDDFGDGY TRFHEDLSPR
310 320 330 340 350
PPLEYTTDIP AVFTDPSVME RYYYTLDRQQ SNTDTSWLTP PQLPPQLENV
360 370 380 390 400
ILNKYYATQD QFNENNSGAL PIPNHVVLNH LVTSSIKHNT LCVASIVRYK
410
QKYVTQILYT PIESS
Length:415
Mass (Da):46,405
Last modified:January 23, 2007 - v3
Checksum:iCBB4FCE0070A563F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z14128 Genomic DNA. Translation: CAA78503.1.
L31592 Genomic DNA. Translation: AAC37420.1.
Z72730 Genomic DNA. Translation: CAA96922.1.
U33754 Genomic DNA. Translation: AAC49497.1.
BK006941 Genomic DNA. Translation: DAA07908.1.
PIRiS51792.
RefSeqiNP_011307.1. NM_001181073.2.

Genome annotation databases

EnsemblFungiiYGL208W; YGL208W; YGL208W.
GeneIDi852664.
KEGGisce:YGL208W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z14128 Genomic DNA. Translation: CAA78503.1.
L31592 Genomic DNA. Translation: AAC37420.1.
Z72730 Genomic DNA. Translation: CAA96922.1.
U33754 Genomic DNA. Translation: AAC49497.1.
BK006941 Genomic DNA. Translation: DAA07908.1.
PIRiS51792.
RefSeqiNP_011307.1. NM_001181073.2.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2QLVX-ray2.60B/E161-412[»]
3T4NX-ray2.30B304-415[»]
3TDHX-ray2.30B304-415[»]
3TE5X-ray2.50B304-415[»]
ProteinModelPortaliP34164.
SMRiP34164. Positions 161-413.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi33048. 42 interactions.
DIPiDIP-865N.
IntActiP34164. 16 interactions.
MINTiMINT-404843.

Protein family/group databases

CAZyiCBM48. Carbohydrate-Binding Module Family 48.

PTM databases

iPTMnetiP34164.

Proteomic databases

MaxQBiP34164.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYGL208W; YGL208W; YGL208W.
GeneIDi852664.
KEGGisce:YGL208W.

Organism-specific databases

EuPathDBiFungiDB:YGL208W.
SGDiS000003176. SIP2.

Phylogenomic databases

GeneTreeiENSGT00390000001416.
HOGENOMiHOG000093748.
InParanoidiP34164.
OrthoDBiEOG092C4NLT.

Enzyme and pathway databases

BioCyciYEAST:G3O-30685-MONOMER.
ReactomeiR-SCE-163680. AMPK inhibits chREBP transcriptional activation activity.
R-SCE-200425. Import of palmitoyl-CoA into the mitochondrial matrix.
R-SCE-380972. Energy dependent regulation of mTOR by LKB1-AMPK.

Miscellaneous databases

EvolutionaryTraceiP34164.
PROiP34164.

Family and domain databases

InterProiIPR032640. AMPK1_CBM.
IPR006828. ASC_dom.
IPR014756. Ig_E-set.
[Graphical view]
PfamiPF16561. AMPK1_CBM. 1 hit.
PF04739. AMPKBI. 1 hit.
[Graphical view]
SMARTiSM01010. AMPKBI. 1 hit.
[Graphical view]
SUPFAMiSSF81296. SSF81296. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiSIP2_YEAST
AccessioniPrimary (citable) accession number: P34164
Secondary accession number(s): D6VTU7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: January 23, 2007
Last modified: September 7, 2016
This is version 135 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 300 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome VII
    Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.