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P34164

- SIP2_YEAST

UniProt

P34164 - SIP2_YEAST

Protein

SNF1 protein kinase subunit beta-2

Gene

SIP2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 117 (01 Oct 2014)
      Sequence version 3 (23 Jan 2007)
      Previous versions | rss
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    Functioni

    Beta subunit of the SNF1 kinase complex, which is required for transcriptional, metabolic, and developmental adaptations in response to glucose limitation. Has a structural role, mediating heterotrimer formation, and a regulatory role, defining carbon source-regulated subcellular location and substrate specificity of the SNF1 kinase complex. Involved in the regulation of aging. Acts as a negative regulator of nuclear SNF1 activity in young cells by sequestering its activating gamma subunit at the plasma membrane.2 Publications

    GO - Molecular functioni

    1. protein binding Source: IntAct

    GO - Biological processi

    1. cellular response to glucose starvation Source: SGD
    2. invasive growth in response to glucose limitation Source: SGD
    3. protein phosphorylation Source: SGD
    4. regulation of protein complex assembly Source: SGD
    5. replicative cell aging Source: SGD
    6. signal transduction Source: SGD

    Enzyme and pathway databases

    BioCyciYEAST:G3O-30685-MONOMER.
    ReactomeiREACT_209479. Regulation of AMPK activity via LKB1.
    REACT_212098. AMPK inhibits chREBP transcriptional activation activity.

    Protein family/group databases

    CAZyiCBM48. Carbohydrate-Binding Module Family 48.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    SNF1 protein kinase subunit beta-2
    Alternative name(s):
    Protein SPM2
    SNF1-interacting protein 2
    Gene namesi
    Name:SIP2
    Synonyms:SPM2
    Ordered Locus Names:YGL208W
    ORF Names:G1155
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome VII

    Organism-specific databases

    SGDiS000003176. SIP2.

    Subcellular locationi

    Cytoplasm. Cell membrane; Peripheral membrane protein; Cytoplasmic side
    Note: Resides in the cytosol during growth in glucose. Excluded from the nucleus. There is an age-associated shift in localization from the plasma membrane to the cytoplasm.

    GO - Cellular componenti

    1. AMP-activated protein kinase complex Source: SGD
    2. cytoplasm Source: SGD
    3. plasma membrane Source: SGD

    Keywords - Cellular componenti

    Cell membrane, Cytoplasm, Membrane

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi2 – 21G → A: Changes protein distribution from the plasma membrane to the cytoplasm and nucleus and alters the cellular life span. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11Removed
    Chaini2 – 415414SNF1 protein kinase subunit beta-2PRO_0000204375Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Lipidationi2 – 21N-myristoyl glycine1 Publication
    Modified residuei66 – 661Phosphoserine2 Publications
    Modified residuei298 – 2981Phosphoserine2 Publications

    Post-translational modificationi

    Phosphorylated by SNF1 in vitro.2 Publications

    Keywords - PTMi

    Lipoprotein, Myristate, Phosphoprotein

    Proteomic databases

    MaxQBiP34164.
    PaxDbiP34164.
    PeptideAtlasiP34164.

    Expressioni

    Inductioni

    Induced upon shift to nonfermentable carbon sources.1 Publication

    Gene expression databases

    GenevestigatoriP34164.

    Interactioni

    Subunit structurei

    Component of the SNF1 kinase complex, a heterotrimeric complex composed of the catalytic alpha subunit SNF1, one of the three related beta subunits SIP1, SIP2 or GAL83, and the regulatory gamma subunit SNF4. The beta subunit serves as a bridge between the catalytic and the regulatory subunit. Interacts (via KIS domain) with SNF1. Interacts (via ASC domain) with SNF4.5 Publications

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    SNF1P067829EBI-17187,EBI-17516
    SNF4P129046EBI-17187,EBI-17537

    Protein-protein interaction databases

    BioGridi33048. 40 interactions.
    DIPiDIP-865N.
    IntActiP34164. 15 interactions.
    MINTiMINT-404843.
    STRINGi4932.YGL208W.

    Structurei

    Secondary structure

    1
    415
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi164 – 1707
    Beta strandi177 – 1815
    Helixi182 – 1843
    Beta strandi196 – 1983
    Beta strandi202 – 2087
    Beta strandi210 – 21910
    Beta strandi222 – 2243
    Beta strandi231 – 2333
    Beta strandi240 – 2456
    Beta strandi305 – 3073
    Helixi311 – 3133
    Helixi316 – 3249
    Helixi336 – 3383
    Helixi345 – 3473
    Helixi349 – 36517
    Helixi375 – 3773
    Beta strandi380 – 3834
    Beta strandi390 – 39910
    Beta strandi402 – 41110

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    2QLVX-ray2.60B/E161-412[»]
    3T4NX-ray2.30B304-415[»]
    3TDHX-ray2.30B304-415[»]
    3TE5X-ray2.50B304-415[»]
    ProteinModelPortaliP34164.
    SMRiP34164. Positions 161-413.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP34164.

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni154 – 335182Kinase-interacting sequence (KIS); required for interaction with SNF1Add
    BLAST
    Regioni336 – 41580Association with SNF1 kinase complex (ASC) domain; required for interaction with SNF4Add
    BLAST

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiNOG238368.
    GeneTreeiENSGT00390000001416.
    HOGENOMiHOG000093748.
    OrthoDBiEOG7BS4NQ.

    Family and domain databases

    InterProiIPR006828. AMP_prot_kin_bsu_interact-dom.
    IPR014756. Ig_E-set.
    [Graphical view]
    PfamiPF04739. AMPKBI. 1 hit.
    [Graphical view]
    SMARTiSM01010. AMPKBI. 1 hit.
    [Graphical view]
    SUPFAMiSSF81296. SSF81296. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P34164-1 [UniParc]FASTAAdd to Basket

    « Hide

    MGTTTSHPAQ KKQTTKKCRA PIMSDVREKP SNAQGCEPQE MDAVSKKVTE    50
    LSLNKCSDSQ DAGQPSREGS ITKKKSTLLL RDEDEPTMPK LSVMETAVDT 100
    DSGSSSTSDD EEGDIIAQTT EPKQDASPDD DRSGHSSPRE EGQQQIRAKE 150
    ASGGPSEIKS SLMVPVEIRW QQGGSKVYVT GSFTKWRKMI GLIPDSDNNG 200
    SFHVKLRLLP GTHRFRFIVD NELRVSDFLP TATDQMGNFV NYIEVRQPEK 250
    NPTNEKIRSK EADSMRPPTS DRSSIALQIG KDPDDFGDGY TRFHEDLSPR 300
    PPLEYTTDIP AVFTDPSVME RYYYTLDRQQ SNTDTSWLTP PQLPPQLENV 350
    ILNKYYATQD QFNENNSGAL PIPNHVVLNH LVTSSIKHNT LCVASIVRYK 400
    QKYVTQILYT PIESS 415
    Length:415
    Mass (Da):46,405
    Last modified:January 23, 2007 - v3
    Checksum:iCBB4FCE0070A563F
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z14128 Genomic DNA. Translation: CAA78503.1.
    L31592 Genomic DNA. Translation: AAC37420.1.
    Z72730 Genomic DNA. Translation: CAA96922.1.
    U33754 Genomic DNA. Translation: AAC49497.1.
    BK006941 Genomic DNA. Translation: DAA07908.1.
    PIRiS51792.
    RefSeqiNP_011307.1. NM_001181073.2.

    Genome annotation databases

    EnsemblFungiiYGL208W; YGL208W; YGL208W.
    GeneIDi852664.
    KEGGisce:YGL208W.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z14128 Genomic DNA. Translation: CAA78503.1 .
    L31592 Genomic DNA. Translation: AAC37420.1 .
    Z72730 Genomic DNA. Translation: CAA96922.1 .
    U33754 Genomic DNA. Translation: AAC49497.1 .
    BK006941 Genomic DNA. Translation: DAA07908.1 .
    PIRi S51792.
    RefSeqi NP_011307.1. NM_001181073.2.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    2QLV X-ray 2.60 B/E 161-412 [» ]
    3T4N X-ray 2.30 B 304-415 [» ]
    3TDH X-ray 2.30 B 304-415 [» ]
    3TE5 X-ray 2.50 B 304-415 [» ]
    ProteinModelPortali P34164.
    SMRi P34164. Positions 161-413.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 33048. 40 interactions.
    DIPi DIP-865N.
    IntActi P34164. 15 interactions.
    MINTi MINT-404843.
    STRINGi 4932.YGL208W.

    Protein family/group databases

    CAZyi CBM48. Carbohydrate-Binding Module Family 48.

    Proteomic databases

    MaxQBi P34164.
    PaxDbi P34164.
    PeptideAtlasi P34164.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YGL208W ; YGL208W ; YGL208W .
    GeneIDi 852664.
    KEGGi sce:YGL208W.

    Organism-specific databases

    SGDi S000003176. SIP2.

    Phylogenomic databases

    eggNOGi NOG238368.
    GeneTreei ENSGT00390000001416.
    HOGENOMi HOG000093748.
    OrthoDBi EOG7BS4NQ.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-30685-MONOMER.
    Reactomei REACT_209479. Regulation of AMPK activity via LKB1.
    REACT_212098. AMPK inhibits chREBP transcriptional activation activity.

    Miscellaneous databases

    EvolutionaryTracei P34164.
    NextBioi 971954.

    Gene expression databases

    Genevestigatori P34164.

    Family and domain databases

    InterProi IPR006828. AMP_prot_kin_bsu_interact-dom.
    IPR014756. Ig_E-set.
    [Graphical view ]
    Pfami PF04739. AMPKBI. 1 hit.
    [Graphical view ]
    SMARTi SM01010. AMPKBI. 1 hit.
    [Graphical view ]
    SUPFAMi SSF81296. SSF81296. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Suppressors of yeast RNA polymerase II mutations belong to a family of gene products that interact with a protein kinase."
      Drebot M.A., Jansma D., Himmelfarb H.J., Friesen J.D.
      Submitted (JUL-1992) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. "A family of proteins containing a conserved domain that mediates interaction with the yeast SNF1 protein kinase complex."
      Yang X., Jiang R., Carlson M.
      EMBO J. 13:5878-5886(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], INTERACTION WITH SNF1, PHOSPHORYLATION.
      Strain: ATCC 204508 / S288c.
    3. "Analysis of 21.7 kb DNA sequence from the left arm of chromosome VII reveals 11 open reading frames: two correspond to new genes."
      Feuermann M., Simeonava L., Souciet J.-L., Potier S.
      Yeast 13:475-477(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    4. "The nucleotide sequence of Saccharomyces cerevisiae chromosome VII."
      Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J., Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M., Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L., Coblenz A., Coglievina M., Coissac E.
      , Defoor E., Del Bino S., Delius H., Delneri D., de Wergifosse P., Dujon B., Durand P., Entian K.-D., Eraso P., Escribano V., Fabiani L., Fartmann B., Feroli F., Feuermann M., Frontali L., Garcia-Gonzalez M., Garcia-Saez M.I., Goffeau A., Guerreiro P., Hani J., Hansen M., Hebling U., Hernandez K., Heumann K., Hilger F., Hofmann B., Indge K.J., James C.M., Klima R., Koetter P., Kramer B., Kramer W., Lauquin G., Leuther H., Louis E.J., Maillier E., Marconi A., Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K., Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S., Nawrocki A., Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L., Paoluzi S., Plevani P., Portetelle D., Portillo F., Potier S., Purnelle B., Rieger M., Riles L., Rinaldi T., Robben J., Rodrigues-Pousada C., Rodriguez-Belmonte E., Rodriguez-Torres A.M., Rose M., Ruzzi M., Saliola M., Sanchez-Perez M., Schaefer B., Schaefer M., Scharfe M., Schmidheini T., Schreer A., Skala J., Souciet J.-L., Steensma H.Y., Talla E., Thierry A., Vandenbol M., van der Aart Q.J.M., Van Dyck L., Vanoni M., Verhasselt P., Voet M., Volckaert G., Wambutt R., Watson M.D., Weber N., Wedler E., Wedler H., Wipfli P., Wolf K., Wright L.F., Zaccaria P., Zimmermann M., Zollner A., Kleine K.
      Nature 387:81-84(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    5. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    6. "Lambda clone B22 contains a 7676 bp genomic fragment of Saccharomyces cerevisiae chromosome VII spanning the VAM7-SPM2 intergenic region and containing three novel transcribed open reading frames."
      Kail M., Juettner E., Vaux D.
      Yeast 12:799-807(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-284.
      Strain: ATCC 204508 / S288c.
    7. "The Snf1 protein kinase and its activating subunit, Snf4, interact with distinct domains of the Sip1/Sip2/Gal83 component in the kinase complex."
      Jiang R., Carlson M.
      Mol. Cell. Biol. 17:2099-2106(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH SNF1 AND SNF4.
    8. "beta-subunits of Snf1 kinase are required for kinase function and substrate definition."
      Schmidt M.C., McCartney R.R.
      EMBO J. 19:4936-4943(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    9. "Subcellular localization of the Snf1 kinase is regulated by specific beta subunits and a novel glucose signaling mechanism."
      Vincent O., Townley R., Kuchin S., Carlson M.
      Genes Dev. 15:1104-1114(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION, INDUCTION.
    10. "Purification and characterization of Snf1 kinase complexes containing a defined beta subunit composition."
      Nath N., McCartney R.R., Schmidt M.C.
      J. Biol. Chem. 277:50403-50408(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION IN SNF1 KINASE COMPLEX.
    11. "Sip2, an N-myristoylated beta subunit of Snf1 kinase, regulates aging in Saccharomyces cerevisiae by affecting cellular histone kinase activity, recombination at rDNA loci, and silencing."
      Lin S.S., Manchester J.K., Gordon J.I.
      J. Biol. Chem. 278:13390-13397(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, MYRISTOYLATION AT GLY-2, SUBCELLULAR LOCATION, MUTAGENESIS OF GLY-2.
    12. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    13. "Analysis of phosphorylation sites on proteins from Saccharomyces cerevisiae by electron transfer dissociation (ETD) mass spectrometry."
      Chi A., Huttenhower C., Geer L.Y., Coon J.J., Syka J.E.P., Bai D.L., Shabanowitz J., Burke D.J., Troyanskaya O.G., Hunt D.F.
      Proc. Natl. Acad. Sci. U.S.A. 104:2193-2198(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    14. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
      Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
      Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-66 AND SER-298, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    15. "Crystal structure of the heterotrimer core of Saccharomyces cerevisiae AMPK homologue SNF1."
      Amodeo G.A., Rudolph M.J., Tong L.
      Nature 449:492-495(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.6 ANGSTROMS) OF 161-412 IN COMPLEX WITH SNF1 AND SNF4.
    16. Cited for: X-RAY CRYSTALLOGRAPHY (2.30 ANGSTROMS) OF 304-415 IN COMPLEX WITH SNF1 AND SNF4.

    Entry informationi

    Entry nameiSIP2_YEAST
    AccessioniPrimary (citable) accession number: P34164
    Secondary accession number(s): D6VTU7
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1994
    Last sequence update: January 23, 2007
    Last modified: October 1, 2014
    This is version 117 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 300 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families
    3. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    4. Yeast chromosome VII
      Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names

    External Data

    Dasty 3