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Protein

Sterol esterase TGL1

Gene

TGL1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Mediates the hydrolysis of steryl esters. Required for mobilization of steryl ester, thereby playing a central role in lipid metabolism. May have weak lipase activity toward triglycerides upon some conditions, however, the relevance of such activity is unclear in vivo.4 Publications

Catalytic activityi

A steryl ester + H2O = a sterol + a fatty acid.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei201 – 2011NucleophileBy similarity
Active sitei369 – 3691Charge relay systemBy similarity
Active sitei396 – 3961Charge relay systemBy similarity

GO - Molecular functioni

  • lipase activity Source: SGD
  • sterol esterase activity Source: SGD

GO - Biological processi

  • cellular lipid metabolic process Source: SGD
  • lipid catabolic process Source: UniProtKB-KW
  • sterol metabolic process Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Lipid degradation, Lipid metabolism

Enzyme and pathway databases

BioCyciMetaCyc:YKL140W-MONOMER.
YEAST:YKL140W-MONOMER.
BRENDAi3.1.1.13. 984.
ReactomeiR-SCE-192456. Digestion of dietary lipid.

Protein family/group databases

ESTHERiyeast-tgl1. Acidic_Lipase.

Names & Taxonomyi

Protein namesi
Recommended name:
Sterol esterase TGL1 (EC:3.1.1.13)
Alternative name(s):
Triglyceride lipase-cholesterol esterase 1
Gene namesi
Name:TGL1
Ordered Locus Names:YKL140W
ORF Names:YKL5
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XI

Organism-specific databases

EuPathDBiFungiDB:YKL140W.
SGDiS000001623. TGL1.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 1313LumenalSequence analysisAdd
BLAST
Transmembranei14 – 3421Helical; Signal-anchor for type III membrane proteinSequence analysisAdd
BLAST
Topological domaini35 – 548514CytoplasmicSequence analysisAdd
BLAST

GO - Cellular componenti

  • integral component of membrane Source: SGD
  • lipid particle Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Lipid droplet, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 548548Sterol esterase TGL1PRO_0000090379Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Cross-linki246 – 246Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)Combined sources
Modified residuei462 – 4621PhosphoserineCombined sources
Modified residuei466 – 4661PhosphoserineCombined sources
Modified residuei521 – 5211PhosphoserineCombined sources
Modified residuei538 – 5381PhosphoserineCombined sources
Modified residuei539 – 5391PhosphothreonineCombined sources

Post-translational modificationi

Not N-glycosylated.

Keywords - PTMi

Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

MaxQBiP34163.
TopDownProteomicsiP34163.

PTM databases

iPTMnetiP34163.

Interactioni

Protein-protein interaction databases

BioGridi33996. 37 interactions.
DIPiDIP-6306N.
IntActiP34163. 11 interactions.
MINTiMINT-4493007.

Structurei

3D structure databases

ProteinModelPortaliP34163.
SMRiP34163. Positions 66-417.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the AB hydrolase superfamily.Curated

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

GeneTreeiENSGT00550000074328.
HOGENOMiHOG000173756.
InParanoidiP34163.
KOiK01052.
OMAiCIDIANK.
OrthoDBiEOG092C1FMW.

Family and domain databases

Gene3Di3.40.50.1820. 1 hit.
InterProiIPR029058. AB_hydrolase.
IPR000073. AB_hydrolase_1.
[Graphical view]
PfamiPF00561. Abhydrolase_1. 1 hit.
[Graphical view]
SUPFAMiSSF53474. SSF53474. 1 hit.

Sequencei

Sequence statusi: Complete.

P34163-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MYFPFLGRLS ITDYIIVVLV YIESIISSVL KLIPQPMINL FEWLINFSTS
60 70 80 90 100
SDDNTIEEKL RSAPTIHEMC AIFDISVEDH LVRTEDNYIL TLHRIPPISK
110 120 130 140 150
NRFNNKVVYL HHGLLMCSDV WCCNIERHKN LPFVLHDLGY DVWMGNNRGN
160 170 180 190 200
KYSTAHLNKP PKSNKFWDFS IDEFAFFDIP NSIEFILDIT KVDKVICIGF
210 220 230 240 250
SQGSAQMFAA FSLSEKLNRK VSHFIAIAPA MTPKGLHNRI VDTLAKSSPG
260 270 280 290 300
FMYLFFGRKI VLPSAVIWQR TLHPTLFNLC IDIANKILFN WKSFNILPRQ
310 320 330 340 350
KIASYAKLYS TTSVKSIVHW FQILRSQKFQ MFEESDNMLN SLTRPYQIAN
360 370 380 390 400
FPTRTNIKIP ILLIYGGIDS LVDIDVMKKN LPFNSVFDVK VDNYEHLDLI
410 420 430 440 450
WGKDADTLVI AKVLRFIEFF NPGNVSVKTN QLLPSASLVE ELPSTTWKTT
460 470 480 490 500
HPTHGLSYRT HSADRSPLSV QADEADEVHN ADNSRFLRRV FSTSAIDEDN
510 520 530 540
ENEHQDDTED QIHKEQQRRL SAYLESSKDL RQLDANSSTT ALDALNKE
Length:548
Mass (Da):62,979
Last modified:February 1, 1994 - v1
Checksum:i32D1F230701CB083
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z25464 Genomic DNA. Translation: CAA80958.1.
Z28140 Genomic DNA. Translation: CAA81981.1.
BK006944 Genomic DNA. Translation: DAA09022.1.
PIRiS37969.
RefSeqiNP_012782.1. NM_001179706.1.

Genome annotation databases

EnsemblFungiiYKL140W; YKL140W; YKL140W.
GeneIDi853717.
KEGGisce:YKL140W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z25464 Genomic DNA. Translation: CAA80958.1.
Z28140 Genomic DNA. Translation: CAA81981.1.
BK006944 Genomic DNA. Translation: DAA09022.1.
PIRiS37969.
RefSeqiNP_012782.1. NM_001179706.1.

3D structure databases

ProteinModelPortaliP34163.
SMRiP34163. Positions 66-417.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi33996. 37 interactions.
DIPiDIP-6306N.
IntActiP34163. 11 interactions.
MINTiMINT-4493007.

Protein family/group databases

ESTHERiyeast-tgl1. Acidic_Lipase.

PTM databases

iPTMnetiP34163.

Proteomic databases

MaxQBiP34163.
TopDownProteomicsiP34163.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYKL140W; YKL140W; YKL140W.
GeneIDi853717.
KEGGisce:YKL140W.

Organism-specific databases

EuPathDBiFungiDB:YKL140W.
SGDiS000001623. TGL1.

Phylogenomic databases

GeneTreeiENSGT00550000074328.
HOGENOMiHOG000173756.
InParanoidiP34163.
KOiK01052.
OMAiCIDIANK.
OrthoDBiEOG092C1FMW.

Enzyme and pathway databases

BioCyciMetaCyc:YKL140W-MONOMER.
YEAST:YKL140W-MONOMER.
BRENDAi3.1.1.13. 984.
ReactomeiR-SCE-192456. Digestion of dietary lipid.

Miscellaneous databases

PROiP34163.

Family and domain databases

Gene3Di3.40.50.1820. 1 hit.
InterProiIPR029058. AB_hydrolase.
IPR000073. AB_hydrolase_1.
[Graphical view]
PfamiPF00561. Abhydrolase_1. 1 hit.
[Graphical view]
SUPFAMiSSF53474. SSF53474. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiTGL1_YEAST
AccessioniPrimary (citable) accession number: P34163
Secondary accession number(s): D6VX56
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: February 1, 1994
Last modified: September 7, 2016
This is version 138 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 1470 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XI
    Yeast (Saccharomyces cerevisiae) chromosome XI: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.