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Protein

Mediator of RNA polymerase II transcription subunit 20

Gene

SRB2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. The Mediator complex, having a compact conformation in its free form, is recruited to promoters by direct interactions with regulatory proteins and serves for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. The Mediator complex unfolds to an extended conformation and partially surrounds RNA polymerase II, specifically interacting with the unphosphorylated form of the C-terminal domain (CTD) of RNA polymerase II. The Mediator complex dissociates from the RNA polymerase II holoenzyme and stays at the promoter when transcriptional elongation begins.4 Publications

Miscellaneous

Present with 1720 molecules/cell in log phase SD medium.1 Publication

GO - Molecular functioni

  • protein domain specific binding Source: SGD
  • RNA polymerase II transcription coactivator activity involved in preinitiation complex assembly Source: SGD
  • RNA polymerase II transcription factor activity, TBP-class protein binding, involved in preinitiation complex assembly Source: SGD
  • transcription factor activity, core RNA polymerase II binding Source: SGD
  • transcription factor activity, RNA polymerase II transcription factor recruiting Source: SGD

GO - Biological processi

Keywordsi

Molecular functionActivator
Biological processTranscription, Transcription regulation

Enzyme and pathway databases

BioCyciYEAST:G3O-31100-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Mediator of RNA polymerase II transcription subunit 20
Alternative name(s):
Hyper-recombination suppressor protein 2
Mediator complex subunit 20
Suppressor of RNA polymerase B 2
Gene namesi
Name:SRB2
Synonyms:HRS2, MED20
Ordered Locus Names:YHR041C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome VIII

Organism-specific databases

EuPathDBiFungiDB:YHR041C
SGDiS000001083 SRB2

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi14P → H in SRB2-1; suppresses the phenotypic defects of an RNA polymerase II CTD truncation. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000963741 – 210Mediator of RNA polymerase II transcription subunit 20Add BLAST210

Proteomic databases

MaxQBiP34162
PaxDbiP34162
PRIDEiP34162

Interactioni

Subunit structurei

Component of the Mediator complex, which is composed of at least 21 subunits that form three structurally distinct submodules. The Mediator head module contains MED6, MED8, MED11, SRB4/MED17, SRB5/MED18, ROX3/MED19, SRB2/MED20 and SRB6/MED22, the middle module contains MED1, MED4, NUT1/MED5, MED7, CSE2/MED9, NUT2/MED10, SRB7/MED21 and SOH1/MED31, and the tail module contains MED2, PGD1/MED3, RGR1/MED14, GAL11/MED15 and SIN4/MED16. The head and the middle modules interact directly with RNA polymerase II, whereas the elongated tail module interacts with gene-specific regulatory proteins. MED1 interacts directly with MED4 and MED7. SRB2/MED20 interacts directly with SRB4/MED17 and SRB5/MED18.4 Publications

Binary interactionsi

Show more details

GO - Molecular functioni

  • protein domain specific binding Source: SGD

Protein-protein interaction databases

BioGridi36473, 648 interactors
DIPiDIP-107N
IntActiP34162, 33 interactors
MINTiP34162
STRINGi4932.YHR041C

Structurei

Secondary structure

1210
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi4 – 9Combined sources6
Helixi16 – 24Combined sources9
Helixi25 – 27Combined sources3
Beta strandi30 – 32Combined sources3
Beta strandi35 – 44Combined sources10
Beta strandi51 – 53Combined sources3
Beta strandi56 – 73Combined sources18
Beta strandi76 – 81Combined sources6
Helixi84 – 86Combined sources3
Helixi89 – 93Combined sources5
Helixi104 – 111Combined sources8
Turni113 – 115Combined sources3
Beta strandi116 – 132Combined sources17
Beta strandi135 – 144Combined sources10
Beta strandi147 – 158Combined sources12
Helixi160 – 162Combined sources3
Helixi163 – 176Combined sources14
Beta strandi183 – 185Combined sources3
Helixi196 – 208Combined sources13

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2HZMX-ray2.40A/C/E/G1-210[»]
2HZSX-ray2.70A/C/E/G2-210[»]
3J1Oelectron microscopy16.00M1-210[»]
3RJ1X-ray4.30F/M/T1-210[»]
4GWPX-ray4.20F1-210[»]
4GWQX-ray4.50F1-210[»]
4V1Oelectron microscopy9.70Y2-210[»]
5SVAelectron microscopy15.30R1-210[»]
ProteinModelPortaliP34162
SMRiP34162
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP34162

Family & Domainsi

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi1 – 210Ser/Thr/Tyr-richAdd BLAST210

Sequence similaritiesi

Belongs to the Mediator complex subunit 20 family.Curated

Phylogenomic databases

HOGENOMiHOG000113535
InParanoidiP34162
OMAiKIFRNNP
OrthoDBiEOG092C56VU

Family and domain databases

InterProiView protein in InterPro
IPR016532 Med20
IPR013921 Mediator_Med20
PfamiView protein in Pfam
PF08612 Med20, 1 hit
PIRSFiPIRSF007945 SRB2, 1 hit

Sequencei

Sequence statusi: Complete.

P34162-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGKSAVIFVE RATPATLTEL KDALSNSILS VRDPWSIDFR TYRCSIKNLP
60 70 80 90 100
ADVSKLMYSI TFHHHGRQTV LIKDNSAMVT TAAAADIPPA LVFNGSSTGV
110 120 130 140 150
PESIDTILSS KLSNIWMQRQ LIKGDAGETL ILDGLTVRLV NLFSSTGFKG
160 170 180 190 200
LLIELQADEA GEFETKIAGI EGHLAEIRAK EYKTSSDSLG PDTSNEICDL
210
AYQYVRALEL
Length:210
Mass (Da):22,894
Last modified:February 1, 1994 - v1
Checksum:iC30BF309D43AFB2A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
S36754 Genomic DNA Translation: AAB22223.1
U00062 Genomic DNA Translation: AAB68920.1
BK006934 Genomic DNA Translation: DAA06733.1
PIRiA38105
RefSeqiNP_011907.1, NM_001179171.1

Genome annotation databases

EnsemblFungiiYHR041C; YHR041C; YHR041C
GeneIDi856437
KEGGisce:YHR041C

Similar proteinsi

Entry informationi

Entry nameiMED20_YEAST
AccessioniPrimary (citable) accession number: P34162
Secondary accession number(s): D3DKY9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: February 1, 1994
Last modified: March 28, 2018
This is version 150 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome
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Main funding by: National Institutes of Health