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P34162

- MED20_YEAST

UniProt

P34162 - MED20_YEAST

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Protein

Mediator of RNA polymerase II transcription subunit 20

Gene

SRB2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. The Mediator complex, having a compact conformation in its free form, is recruited to promoters by direct interactions with regulatory proteins and serves for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. The Mediator complex unfolds to an extended conformation and partially surrounds RNA polymerase II, specifically interacting with the unphosphorylated form of the C-terminal domain (CTD) of RNA polymerase II. The Mediator complex dissociates from the RNA polymerase II holoenzyme and stays at the promoter when transcriptional elongation begins.4 Publications

GO - Molecular functioni

  1. core RNA polymerase II binding transcription factor activity Source: SGD
  2. protein domain specific binding Source: SGD
  3. RNA polymerase II transcription coactivator activity involved in preinitiation complex assembly Source: SGD
  4. RNA polymerase II transcription factor recruiting transcription factor activity Source: SGD
  5. TBP-class protein binding RNA polymerase II transcription factor activity involved in preinitiation complex assembly Source: SGD

GO - Biological processi

  1. negative regulation of ribosomal protein gene transcription from RNA polymerase II promoter in response to chemical stimulus Source: SGD
  2. negative regulation of ribosomal protein gene transcription from RNA polymerase II promoter in response to nutrient levels Source: SGD
  3. positive regulation of transcription from RNA polymerase II promoter Source: SGD
  4. RNA polymerase II transcriptional preinitiation complex assembly Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Activator

Keywords - Biological processi

Transcription, Transcription regulation

Enzyme and pathway databases

BioCyciYEAST:G3O-31100-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Mediator of RNA polymerase II transcription subunit 20
Alternative name(s):
Hyper-recombination suppressor protein 2
Mediator complex subunit 20
Suppressor of RNA polymerase B 2
Gene namesi
Name:SRB2
Synonyms:HRS2, MED20
Ordered Locus Names:YHR041C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome VIII

Organism-specific databases

CYGDiYHR041c.
SGDiS000001083. SRB2.

Subcellular locationi

Nucleus 1 Publication

GO - Cellular componenti

  1. core mediator complex Source: SGD
  2. mediator complex Source: InterPro
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi14 – 141P → H in SRB2-1; suppresses the phenotypic defects of an RNA polymerase II CTD truncation. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 210210Mediator of RNA polymerase II transcription subunit 20PRO_0000096374Add
BLAST

Proteomic databases

MaxQBiP34162.
PaxDbiP34162.
PeptideAtlasiP34162.

Expressioni

Gene expression databases

GenevestigatoriP34162.

Interactioni

Subunit structurei

Component of the Mediator complex, which is composed of at least 21 subunits that form three structurally distinct submodules. The Mediator head module contains MED6, MED8, MED11, SRB4/MED17, SRB5/MED18, ROX3/MED19, SRB2/MED20 and SRB6/MED22, the middle module contains MED1, MED4, NUT1/MED5, MED7, CSE2/MED9, NUT2/MED10, SRB7/MED21 and SOH1/MED31, and the tail module contains MED2, PGD1/MED3, RGR1/MED14, GAL11/MED15 and SIN4/MED16. The head and the middle modules interact directly with RNA polymerase II, whereas the elongated tail module interacts with gene-specific regulatory proteins. MED1 interacts directly with MED4 and MED7. SRB2/MED20 interacts directly with SRB4/MED17 and SRB5/MED18.4 Publications

Protein-protein interaction databases

BioGridi36473. 304 interactions.
DIPiDIP-107N.
IntActiP34162. 18 interactions.
MINTiMINT-473266.
STRINGi4932.YHR041C.

Structurei

Secondary structure

1
210
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi4 – 96Combined sources
Helixi16 – 249Combined sources
Helixi25 – 273Combined sources
Beta strandi30 – 323Combined sources
Beta strandi35 – 4410Combined sources
Beta strandi51 – 533Combined sources
Beta strandi56 – 7318Combined sources
Beta strandi76 – 816Combined sources
Helixi84 – 863Combined sources
Helixi89 – 935Combined sources
Helixi104 – 1118Combined sources
Turni113 – 1153Combined sources
Beta strandi116 – 13217Combined sources
Beta strandi135 – 14410Combined sources
Beta strandi147 – 15812Combined sources
Helixi160 – 1623Combined sources
Helixi163 – 17614Combined sources
Beta strandi183 – 1853Combined sources
Helixi196 – 20813Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2HZMX-ray2.40A/C/E/G1-210[»]
2HZSX-ray2.70A/C/E/G2-210[»]
3J1Oelectron microscopy16.00M1-210[»]
3RJ1X-ray4.30F/M/T1-210[»]
4GWPX-ray4.20F1-210[»]
4GWQX-ray4.50F1-210[»]
ProteinModelPortaliP34162.
SMRiP34162. Positions 2-210.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP34162.

Family & Domainsi

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi1 – 210210Ser/Thr/Tyr-richAdd
BLAST

Sequence similaritiesi

Belongs to the Mediator complex subunit 20 family.Curated

Phylogenomic databases

eggNOGiNOG41765.
HOGENOMiHOG000113535.
InParanoidiP34162.
OMAiFKIFRNN.
OrthoDBiEOG7GTTFC.

Family and domain databases

InterProiIPR013921. Mediator_Med20.
IPR016532. Mediator_Med20_fun.
[Graphical view]
PfamiPF08612. Med20. 1 hit.
[Graphical view]
PIRSFiPIRSF007945. SRB2. 1 hit.

Sequencei

Sequence statusi: Complete.

P34162-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MGKSAVIFVE RATPATLTEL KDALSNSILS VRDPWSIDFR TYRCSIKNLP
60 70 80 90 100
ADVSKLMYSI TFHHHGRQTV LIKDNSAMVT TAAAADIPPA LVFNGSSTGV
110 120 130 140 150
PESIDTILSS KLSNIWMQRQ LIKGDAGETL ILDGLTVRLV NLFSSTGFKG
160 170 180 190 200
LLIELQADEA GEFETKIAGI EGHLAEIRAK EYKTSSDSLG PDTSNEICDL
210
AYQYVRALEL
Length:210
Mass (Da):22,894
Last modified:February 1, 1994 - v1
Checksum:iC30BF309D43AFB2A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
S36754 Genomic DNA. Translation: AAB22223.1.
U00062 Genomic DNA. Translation: AAB68920.1.
BK006934 Genomic DNA. Translation: DAA06733.1.
PIRiA38105.
RefSeqiNP_011907.1. NM_001179171.1.

Genome annotation databases

EnsemblFungiiYHR041C; YHR041C; YHR041C.
GeneIDi856437.
KEGGisce:YHR041C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
S36754 Genomic DNA. Translation: AAB22223.1 .
U00062 Genomic DNA. Translation: AAB68920.1 .
BK006934 Genomic DNA. Translation: DAA06733.1 .
PIRi A38105.
RefSeqi NP_011907.1. NM_001179171.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
2HZM X-ray 2.40 A/C/E/G 1-210 [» ]
2HZS X-ray 2.70 A/C/E/G 2-210 [» ]
3J1O electron microscopy 16.00 M 1-210 [» ]
3RJ1 X-ray 4.30 F/M/T 1-210 [» ]
4GWP X-ray 4.20 F 1-210 [» ]
4GWQ X-ray 4.50 F 1-210 [» ]
ProteinModelPortali P34162.
SMRi P34162. Positions 2-210.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 36473. 304 interactions.
DIPi DIP-107N.
IntActi P34162. 18 interactions.
MINTi MINT-473266.
STRINGi 4932.YHR041C.

Proteomic databases

MaxQBi P34162.
PaxDbi P34162.
PeptideAtlasi P34162.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YHR041C ; YHR041C ; YHR041C .
GeneIDi 856437.
KEGGi sce:YHR041C.

Organism-specific databases

CYGDi YHR041c.
SGDi S000001083. SRB2.

Phylogenomic databases

eggNOGi NOG41765.
HOGENOMi HOG000113535.
InParanoidi P34162.
OMAi FKIFRNN.
OrthoDBi EOG7GTTFC.

Enzyme and pathway databases

BioCyci YEAST:G3O-31100-MONOMER.

Miscellaneous databases

EvolutionaryTracei P34162.
NextBioi 982039.

Gene expression databases

Genevestigatori P34162.

Family and domain databases

InterProi IPR013921. Mediator_Med20.
IPR016532. Mediator_Med20_fun.
[Graphical view ]
Pfami PF08612. Med20. 1 hit.
[Graphical view ]
PIRSFi PIRSF007945. SRB2. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "A novel transcription factor reveals a functional link between the RNA polymerase II CTD and TFIID."
    Koleske A.J., Buratowski S., Nonet M., Young R.A.
    Cell 69:883-894(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], MUTAGENESIS OF PRO-14.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  4. "A multisubunit complex associated with the RNA polymerase II CTD and TATA-binding protein in yeast."
    Thompson C.M., Koleske A.J., Chao D.M., Young R.A.
    Cell 73:1361-1375(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: CHARACTERIZATION.
  5. "A multiprotein mediator of transcriptional activation and its interaction with the C-terminal repeat domain of RNA polymerase II."
    Kim Y.-J., Bjoerklund S., Li Y., Sayre M.H., Kornberg R.D.
    Cell 77:599-608(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: COMPONENT OF MEDIATOR COMPLEX.
  6. "An activator target in the RNA polymerase II holoenzyme."
    Koh S.S., Ansari A.Z., Ptashne M., Young R.A.
    Mol. Cell 1:895-904(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH SRB4 AND SRB5.
  7. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
  8. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  9. Cited for: NOMENCLATURE.
  10. Cited for: TOPOLOGY OF THE MEDIATOR COMPLEX.
  11. "Preponderance of free mediator in the yeast Saccharomyces cerevisiae."
    Takagi Y., Chadick J.Z., Davis J.A., Asturias F.J.
    J. Biol. Chem. 280:31200-31207(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: CHARACTERIZATION OF THE MEDIATOR COMPLEX.
  12. "Mediator and TFIIH govern carboxyl-terminal domain-dependent transcription in yeast extracts."
    Nair D., Kim Y., Myers L.C.
    J. Biol. Chem. 280:33739-33748(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION OF THE MEDIATOR COMPLEX.
  13. "Mediator expression profiling epistasis reveals a signal transduction pathway with antagonistic submodules and highly specific downstream targets."
    van de Peppel J., Kettelarij N., van Bakel H., Kockelkorn T.T.J.P., van Leenen D., Holstege F.C.P.
    Mol. Cell 19:511-522(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  14. "Mediator as a general transcription factor."
    Takagi Y., Kornberg R.D.
    J. Biol. Chem. 281:80-89(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION OF THE MEDIATOR COMPLEX.
  15. "Med19(Rox3) regulates intermodule interactions in the Saccharomyces cerevisiae mediator complex."
    Baidoobonso S.M., Guidi B.W., Myers L.C.
    J. Biol. Chem. 282:5551-5559(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: CHARACTERIZATION OF THE MEDIATOR COMPLEX, INTERACTION OF THE MEDIATOR COMPLEX WITH RNA POLYMERASE II.
  16. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  17. "Structure of the yeast RNA polymerase II holoenzyme: mediator conformation and polymerase interaction."
    Davis J.A., Takagi Y., Kornberg R.D., Asturias F.J.
    Mol. Cell 10:409-415(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: ELECTRON MICROSCOPY OF MEDIATOR COMPLEX IN COMPLEX WITH RNA POLYMERASE II.
  18. Cited for: ELECTRON MICROSCOPY OF THE MEDIATOR COMPLEX HEAD MODULE, FUNCTION OF THE MEDIATOR COMPLEX HEAD MODULE, INTERACTION OF THE MEDIATOR COMPLEX HEAD MODULE WITH RNA POLYMERASE II AND TFIIF, INTERACTION WITH MED8; SRB4 AND SRB5.
  19. "Structure and TBP binding of the Mediator head subcomplex Med8-Med18-Med20."
    Lariviere L., Geiger S., Hoeppner S., Roether S., Straesser K., Cramer P.
    Nat. Struct. Mol. Biol. 13:895-901(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.7 ANGSTROMS) OF 2-210 IN COMPLEX WITH MED8 AND SRB5, X-RAY CRYSTALLOGRAPHY (2.4 ANGSTROMS) IN COMPLEX WITH SRB5.

Entry informationi

Entry nameiMED20_YEAST
AccessioniPrimary (citable) accession number: P34162
Secondary accession number(s): D3DKY9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: February 1, 1994
Last modified: November 26, 2014
This is version 124 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 1720 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome VIII
    Yeast (Saccharomyces cerevisiae) chromosome VIII: entries and gene names

External Data

Dasty 3