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P34162

- MED20_YEAST

UniProt

P34162 - MED20_YEAST

Protein

Mediator of RNA polymerase II transcription subunit 20

Gene

SRB2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 122 (01 Oct 2014)
      Sequence version 1 (01 Feb 1994)
      Previous versions | rss
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    Functioni

    Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. The Mediator complex, having a compact conformation in its free form, is recruited to promoters by direct interactions with regulatory proteins and serves for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. The Mediator complex unfolds to an extended conformation and partially surrounds RNA polymerase II, specifically interacting with the unphosphorylated form of the C-terminal domain (CTD) of RNA polymerase II. The Mediator complex dissociates from the RNA polymerase II holoenzyme and stays at the promoter when transcriptional elongation begins.4 Publications

    GO - Molecular functioni

    1. core RNA polymerase II binding transcription factor activity Source: SGD
    2. protein domain specific binding Source: SGD
    3. RNA polymerase II transcription coactivator activity involved in preinitiation complex assembly Source: SGD
    4. RNA polymerase II transcription factor recruiting transcription factor activity Source: SGD
    5. TBP-class protein binding RNA polymerase II transcription factor activity involved in preinitiation complex assembly Source: SGD

    GO - Biological processi

    1. negative regulation of ribosomal protein gene transcription from RNA polymerase II promoter in response to chemical stimulus Source: SGD
    2. negative regulation of ribosomal protein gene transcription from RNA polymerase II promoter in response to nutrient levels Source: SGD
    3. positive regulation of transcription from RNA polymerase II promoter Source: SGD
    4. RNA polymerase II transcriptional preinitiation complex assembly Source: SGD

    Keywords - Molecular functioni

    Activator

    Keywords - Biological processi

    Transcription, Transcription regulation

    Enzyme and pathway databases

    BioCyciYEAST:G3O-31100-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Mediator of RNA polymerase II transcription subunit 20
    Alternative name(s):
    Hyper-recombination suppressor protein 2
    Mediator complex subunit 20
    Suppressor of RNA polymerase B 2
    Gene namesi
    Name:SRB2
    Synonyms:HRS2, MED20
    Ordered Locus Names:YHR041C
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome VIII

    Organism-specific databases

    CYGDiYHR041c.
    SGDiS000001083. SRB2.

    Subcellular locationi

    Nucleus 1 Publication

    GO - Cellular componenti

    1. core mediator complex Source: SGD
    2. mediator complex Source: InterPro

    Keywords - Cellular componenti

    Nucleus

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi14 – 141P → H in SRB2-1; suppresses the phenotypic defects of an RNA polymerase II CTD truncation. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 210210Mediator of RNA polymerase II transcription subunit 20PRO_0000096374Add
    BLAST

    Proteomic databases

    MaxQBiP34162.
    PaxDbiP34162.
    PeptideAtlasiP34162.

    Expressioni

    Gene expression databases

    GenevestigatoriP34162.

    Interactioni

    Subunit structurei

    Component of the Mediator complex, which is composed of at least 21 subunits that form three structurally distinct submodules. The Mediator head module contains MED6, MED8, MED11, SRB4/MED17, SRB5/MED18, ROX3/MED19, SRB2/MED20 and SRB6/MED22, the middle module contains MED1, MED4, NUT1/MED5, MED7, CSE2/MED9, NUT2/MED10, SRB7/MED21 and SOH1/MED31, and the tail module contains MED2, PGD1/MED3, RGR1/MED14, GAL11/MED15 and SIN4/MED16. The head and the middle modules interact directly with RNA polymerase II, whereas the elongated tail module interacts with gene-specific regulatory proteins. MED1 interacts directly with MED4 and MED7. SRB2/MED20 interacts directly with SRB4/MED17 and SRB5/MED18.4 Publications

    Protein-protein interaction databases

    BioGridi36473. 304 interactions.
    DIPiDIP-107N.
    IntActiP34162. 18 interactions.
    MINTiMINT-473266.
    STRINGi4932.YHR041C.

    Structurei

    Secondary structure

    1
    210
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi4 – 96
    Helixi16 – 249
    Helixi25 – 273
    Beta strandi30 – 323
    Beta strandi35 – 4410
    Beta strandi51 – 533
    Beta strandi56 – 7318
    Beta strandi76 – 816
    Helixi84 – 863
    Helixi89 – 935
    Helixi104 – 1118
    Turni113 – 1153
    Beta strandi116 – 13217
    Beta strandi135 – 14410
    Beta strandi147 – 15812
    Helixi160 – 1623
    Helixi163 – 17614
    Beta strandi183 – 1853
    Helixi196 – 20813

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    2HZMX-ray2.40A/C/E/G1-210[»]
    2HZSX-ray2.70A/C/E/G2-210[»]
    3J1Oelectron microscopy16.00M1-210[»]
    3RJ1X-ray4.30F/M/T1-210[»]
    4GWPX-ray4.20F1-210[»]
    4GWQX-ray4.50F1-210[»]
    ProteinModelPortaliP34162.
    SMRiP34162. Positions 2-210.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP34162.

    Family & Domainsi

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi1 – 210210Ser/Thr/Tyr-richAdd
    BLAST

    Sequence similaritiesi

    Belongs to the Mediator complex subunit 20 family.Curated

    Phylogenomic databases

    eggNOGiNOG41765.
    HOGENOMiHOG000113535.
    OMAiFKIFRNN.
    OrthoDBiEOG7GTTFC.

    Family and domain databases

    InterProiIPR013921. Mediator_Med20.
    IPR016532. Mediator_Med20_fun.
    [Graphical view]
    PfamiPF08612. Med20. 1 hit.
    [Graphical view]
    PIRSFiPIRSF007945. SRB2. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    P34162-1 [UniParc]FASTAAdd to Basket

    « Hide

    MGKSAVIFVE RATPATLTEL KDALSNSILS VRDPWSIDFR TYRCSIKNLP    50
    ADVSKLMYSI TFHHHGRQTV LIKDNSAMVT TAAAADIPPA LVFNGSSTGV 100
    PESIDTILSS KLSNIWMQRQ LIKGDAGETL ILDGLTVRLV NLFSSTGFKG 150
    LLIELQADEA GEFETKIAGI EGHLAEIRAK EYKTSSDSLG PDTSNEICDL 200
    AYQYVRALEL 210
    Length:210
    Mass (Da):22,894
    Last modified:February 1, 1994 - v1
    Checksum:iC30BF309D43AFB2A
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    S36754 Genomic DNA. Translation: AAB22223.1.
    U00062 Genomic DNA. Translation: AAB68920.1.
    BK006934 Genomic DNA. Translation: DAA06733.1.
    PIRiA38105.
    RefSeqiNP_011907.1. NM_001179171.1.

    Genome annotation databases

    EnsemblFungiiYHR041C; YHR041C; YHR041C.
    GeneIDi856437.
    KEGGisce:YHR041C.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    S36754 Genomic DNA. Translation: AAB22223.1 .
    U00062 Genomic DNA. Translation: AAB68920.1 .
    BK006934 Genomic DNA. Translation: DAA06733.1 .
    PIRi A38105.
    RefSeqi NP_011907.1. NM_001179171.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    2HZM X-ray 2.40 A/C/E/G 1-210 [» ]
    2HZS X-ray 2.70 A/C/E/G 2-210 [» ]
    3J1O electron microscopy 16.00 M 1-210 [» ]
    3RJ1 X-ray 4.30 F/M/T 1-210 [» ]
    4GWP X-ray 4.20 F 1-210 [» ]
    4GWQ X-ray 4.50 F 1-210 [» ]
    ProteinModelPortali P34162.
    SMRi P34162. Positions 2-210.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 36473. 304 interactions.
    DIPi DIP-107N.
    IntActi P34162. 18 interactions.
    MINTi MINT-473266.
    STRINGi 4932.YHR041C.

    Proteomic databases

    MaxQBi P34162.
    PaxDbi P34162.
    PeptideAtlasi P34162.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YHR041C ; YHR041C ; YHR041C .
    GeneIDi 856437.
    KEGGi sce:YHR041C.

    Organism-specific databases

    CYGDi YHR041c.
    SGDi S000001083. SRB2.

    Phylogenomic databases

    eggNOGi NOG41765.
    HOGENOMi HOG000113535.
    OMAi FKIFRNN.
    OrthoDBi EOG7GTTFC.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-31100-MONOMER.

    Miscellaneous databases

    EvolutionaryTracei P34162.
    NextBioi 982039.

    Gene expression databases

    Genevestigatori P34162.

    Family and domain databases

    InterProi IPR013921. Mediator_Med20.
    IPR016532. Mediator_Med20_fun.
    [Graphical view ]
    Pfami PF08612. Med20. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF007945. SRB2. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "A novel transcription factor reveals a functional link between the RNA polymerase II CTD and TFIID."
      Koleske A.J., Buratowski S., Nonet M., Young R.A.
      Cell 69:883-894(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], MUTAGENESIS OF PRO-14.
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    3. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    4. "A multisubunit complex associated with the RNA polymerase II CTD and TATA-binding protein in yeast."
      Thompson C.M., Koleske A.J., Chao D.M., Young R.A.
      Cell 73:1361-1375(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: CHARACTERIZATION.
    5. "A multiprotein mediator of transcriptional activation and its interaction with the C-terminal repeat domain of RNA polymerase II."
      Kim Y.-J., Bjoerklund S., Li Y., Sayre M.H., Kornberg R.D.
      Cell 77:599-608(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: COMPONENT OF MEDIATOR COMPLEX.
    6. "An activator target in the RNA polymerase II holoenzyme."
      Koh S.S., Ansari A.Z., Ptashne M., Young R.A.
      Mol. Cell 1:895-904(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH SRB4 AND SRB5.
    7. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
    8. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    9. Cited for: NOMENCLATURE.
    10. Cited for: TOPOLOGY OF THE MEDIATOR COMPLEX.
    11. "Preponderance of free mediator in the yeast Saccharomyces cerevisiae."
      Takagi Y., Chadick J.Z., Davis J.A., Asturias F.J.
      J. Biol. Chem. 280:31200-31207(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: CHARACTERIZATION OF THE MEDIATOR COMPLEX.
    12. "Mediator and TFIIH govern carboxyl-terminal domain-dependent transcription in yeast extracts."
      Nair D., Kim Y., Myers L.C.
      J. Biol. Chem. 280:33739-33748(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION OF THE MEDIATOR COMPLEX.
    13. "Mediator expression profiling epistasis reveals a signal transduction pathway with antagonistic submodules and highly specific downstream targets."
      van de Peppel J., Kettelarij N., van Bakel H., Kockelkorn T.T.J.P., van Leenen D., Holstege F.C.P.
      Mol. Cell 19:511-522(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    14. "Mediator as a general transcription factor."
      Takagi Y., Kornberg R.D.
      J. Biol. Chem. 281:80-89(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION OF THE MEDIATOR COMPLEX.
    15. "Med19(Rox3) regulates intermodule interactions in the Saccharomyces cerevisiae mediator complex."
      Baidoobonso S.M., Guidi B.W., Myers L.C.
      J. Biol. Chem. 282:5551-5559(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: CHARACTERIZATION OF THE MEDIATOR COMPLEX, INTERACTION OF THE MEDIATOR COMPLEX WITH RNA POLYMERASE II.
    16. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    17. "Structure of the yeast RNA polymerase II holoenzyme: mediator conformation and polymerase interaction."
      Davis J.A., Takagi Y., Kornberg R.D., Asturias F.J.
      Mol. Cell 10:409-415(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: ELECTRON MICROSCOPY OF MEDIATOR COMPLEX IN COMPLEX WITH RNA POLYMERASE II.
    18. Cited for: ELECTRON MICROSCOPY OF THE MEDIATOR COMPLEX HEAD MODULE, FUNCTION OF THE MEDIATOR COMPLEX HEAD MODULE, INTERACTION OF THE MEDIATOR COMPLEX HEAD MODULE WITH RNA POLYMERASE II AND TFIIF, INTERACTION WITH MED8; SRB4 AND SRB5.
    19. "Structure and TBP binding of the Mediator head subcomplex Med8-Med18-Med20."
      Lariviere L., Geiger S., Hoeppner S., Roether S., Straesser K., Cramer P.
      Nat. Struct. Mol. Biol. 13:895-901(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.7 ANGSTROMS) OF 2-210 IN COMPLEX WITH MED8 AND SRB5, X-RAY CRYSTALLOGRAPHY (2.4 ANGSTROMS) IN COMPLEX WITH SRB5.

    Entry informationi

    Entry nameiMED20_YEAST
    AccessioniPrimary (citable) accession number: P34162
    Secondary accession number(s): D3DKY9
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1994
    Last sequence update: February 1, 1994
    Last modified: October 1, 2014
    This is version 122 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 1720 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families
    3. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    4. Yeast chromosome VIII
      Yeast (Saccharomyces cerevisiae) chromosome VIII: entries and gene names

    External Data

    Dasty 3