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P34162 (MED20_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 120. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Mediator of RNA polymerase II transcription subunit 20
Alternative name(s):
Hyper-recombination suppressor protein 2
Mediator complex subunit 20
Suppressor of RNA polymerase B 2
Gene names
Name:SRB2
Synonyms:HRS2, MED20
Ordered Locus Names:YHR041C
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length210 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. The Mediator complex, having a compact conformation in its free form, is recruited to promoters by direct interactions with regulatory proteins and serves for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. The Mediator complex unfolds to an extended conformation and partially surrounds RNA polymerase II, specifically interacting with the unphosphorylated form of the C-terminal domain (CTD) of RNA polymerase II. The Mediator complex dissociates from the RNA polymerase II holoenzyme and stays at the promoter when transcriptional elongation begins. Ref.12 Ref.13 Ref.14 Ref.18

Subunit structure

Component of the Mediator complex, which is composed of at least 21 subunits that form three structurally distinct submodules. The Mediator head module contains MED6, MED8, MED11, SRB4/MED17, SRB5/MED18, ROX3/MED19, SRB2/MED20 and SRB6/MED22, the middle module contains MED1, MED4, NUT1/MED5, MED7, CSE2/MED9, NUT2/MED10, SRB7/MED21 and SOH1/MED31, and the tail module contains MED2, PGD1/MED3, RGR1/MED14, GAL11/MED15 and SIN4/MED16. The head and the middle modules interact directly with RNA polymerase II, whereas the elongated tail module interacts with gene-specific regulatory proteins. MED1 interacts directly with MED4 and MED7. SRB2/MED20 interacts directly with SRB4/MED17 and SRB5/MED18. Ref.6 Ref.15 Ref.18

Subcellular location

Nucleus Ref.7.

Miscellaneous

Present with 1720 molecules/cell in log phase SD medium.

Sequence similarities

Belongs to the Mediator complex subunit 20 family.

Ontologies

Keywords
   Biological processTranscription
Transcription regulation
   Cellular componentNucleus
   Molecular functionActivator
   Technical term3D-structure
Complete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processRNA polymerase II transcriptional preinitiation complex assembly

Inferred from direct assay Ref.1. Source: SGD

negative regulation of ribosomal protein gene transcription from RNA polymerase II promoter in response to chemical stimulus

Inferred from mutant phenotype PubMed 18604275. Source: SGD

negative regulation of ribosomal protein gene transcription from RNA polymerase II promoter in response to nutrient levels

Inferred from mutant phenotype PubMed 18604275. Source: SGD

positive regulation of transcription from RNA polymerase II promoter

Inferred from direct assay Ref.5. Source: SGD

   Cellular_componentcore mediator complex

Inferred from direct assay PubMed 9891034. Source: SGD

mediator complex

Inferred from electronic annotation. Source: InterPro

   Molecular_functionRNA polymerase II transcription coactivator activity involved in preinitiation complex assembly

Inferred from direct assay Ref.1. Source: SGD

RNA polymerase II transcription factor recruiting transcription factor activity

Inferred from mutant phenotype PubMed 15831453. Source: SGD

TBP-class protein binding RNA polymerase II transcription factor activity involved in preinitiation complex assembly

Inferred from direct assay Ref.1. Source: SGD

core RNA polymerase II binding transcription factor activity

Inferred from direct assay PubMed 20154708. Source: SGD

protein domain specific binding

Inferred from genetic interaction PubMed 2693207. Source: SGD

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 210210Mediator of RNA polymerase II transcription subunit 20
PRO_0000096374

Regions

Compositional bias1 – 210210Ser/Thr/Tyr-rich

Experimental info

Mutagenesis141P → H in SRB2-1; suppresses the phenotypic defects of an RNA polymerase II CTD truncation. Ref.1

Secondary structure

.................................... 210
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P34162 [UniParc].

Last modified February 1, 1994. Version 1.
Checksum: C30BF309D43AFB2A

FASTA21022,894
        10         20         30         40         50         60 
MGKSAVIFVE RATPATLTEL KDALSNSILS VRDPWSIDFR TYRCSIKNLP ADVSKLMYSI 

        70         80         90        100        110        120 
TFHHHGRQTV LIKDNSAMVT TAAAADIPPA LVFNGSSTGV PESIDTILSS KLSNIWMQRQ 

       130        140        150        160        170        180 
LIKGDAGETL ILDGLTVRLV NLFSSTGFKG LLIELQADEA GEFETKIAGI EGHLAEIRAK 

       190        200        210 
EYKTSSDSLG PDTSNEICDL AYQYVRALEL 

« Hide

References

« Hide 'large scale' references
[1]"A novel transcription factor reveals a functional link between the RNA polymerase II CTD and TFIID."
Koleske A.J., Buratowski S., Nonet M., Young R.A.
Cell 69:883-894(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], MUTAGENESIS OF PRO-14.
[2]"Complete nucleotide sequence of Saccharomyces cerevisiae chromosome VIII."
Johnston M., Andrews S., Brinkman R., Cooper J., Ding H., Dover J., Du Z., Favello A., Fulton L., Gattung S., Geisel C., Kirsten J., Kucaba T., Hillier L.W., Jier M., Johnston L., Langston Y., Latreille P. expand/collapse author list , Louis E.J., Macri C., Mardis E., Menezes S., Mouser L., Nhan M., Rifkin L., Riles L., St Peter H., Trevaskis E., Vaughan K., Vignati D., Wilcox L., Wohldman P., Waterston R., Wilson R., Vaudin M.
Science 265:2077-2082(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[3]Saccharomyces Genome Database
Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[4]"A multisubunit complex associated with the RNA polymerase II CTD and TATA-binding protein in yeast."
Thompson C.M., Koleske A.J., Chao D.M., Young R.A.
Cell 73:1361-1375(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: CHARACTERIZATION.
[5]"A multiprotein mediator of transcriptional activation and its interaction with the C-terminal repeat domain of RNA polymerase II."
Kim Y.-J., Bjoerklund S., Li Y., Sayre M.H., Kornberg R.D.
Cell 77:599-608(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: COMPONENT OF MEDIATOR COMPLEX.
[6]"An activator target in the RNA polymerase II holoenzyme."
Koh S.S., Ansari A.Z., Ptashne M., Young R.A.
Mol. Cell 1:895-904(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH SRB4 AND SRB5.
[7]"Global analysis of protein localization in budding yeast."
Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K.
Nature 425:686-691(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[8]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[9]"A unified nomenclature for protein subunits of mediator complexes linking transcriptional regulators to RNA polymerase II."
Bourbon H.-M., Aguilera A., Ansari A.Z., Asturias F.J., Berk A.J., Bjoerklund S., Blackwell T.K., Borggrefe T., Carey M., Carlson M., Conaway J.W., Conaway R.C., Emmons S.W., Fondell J.D., Freedman L.P., Fukasawa T., Gustafsson C.M., Han M. expand/collapse author list , He X., Herman P.K., Hinnebusch A.G., Holmberg S., Holstege F.C.P., Jaehning J.A., Kim Y.-J., Kuras L., Leutz A., Lis J.T., Meisterernest M., Naeaer A.M., Nasmyth K., Parvin J.D., Ptashne M., Reinberg D., Ronne H., Sadowski I., Sakurai H., Sipiczki M., Sternberg P.W., Stillman D.J., Strich R., Struhl K., Svejstrup J.Q., Tuck S., Winston F., Roeder R.G., Kornberg R.D.
Mol. Cell 14:553-557(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NOMENCLATURE.
[10]"A high resolution protein interaction map of the yeast Mediator complex."
Guglielmi B., van Berkum N.L., Klapholz B., Bijma T., Boube M., Boschiero C., Bourbon H.-M., Holstege F.C.P., Werner M.
Nucleic Acids Res. 32:5379-5391(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: TOPOLOGY OF THE MEDIATOR COMPLEX.
[11]"Preponderance of free mediator in the yeast Saccharomyces cerevisiae."
Takagi Y., Chadick J.Z., Davis J.A., Asturias F.J.
J. Biol. Chem. 280:31200-31207(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: CHARACTERIZATION OF THE MEDIATOR COMPLEX.
[12]"Mediator and TFIIH govern carboxyl-terminal domain-dependent transcription in yeast extracts."
Nair D., Kim Y., Myers L.C.
J. Biol. Chem. 280:33739-33748(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION OF THE MEDIATOR COMPLEX.
[13]"Mediator expression profiling epistasis reveals a signal transduction pathway with antagonistic submodules and highly specific downstream targets."
van de Peppel J., Kettelarij N., van Bakel H., Kockelkorn T.T.J.P., van Leenen D., Holstege F.C.P.
Mol. Cell 19:511-522(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[14]"Mediator as a general transcription factor."
Takagi Y., Kornberg R.D.
J. Biol. Chem. 281:80-89(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION OF THE MEDIATOR COMPLEX.
[15]"Med19(Rox3) regulates intermodule interactions in the Saccharomyces cerevisiae mediator complex."
Baidoobonso S.M., Guidi B.W., Myers L.C.
J. Biol. Chem. 282:5551-5559(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: CHARACTERIZATION OF THE MEDIATOR COMPLEX, INTERACTION OF THE MEDIATOR COMPLEX WITH RNA POLYMERASE II.
[16]"N-terminal acetylome analyses and functional insights of the N-terminal acetyltransferase NatB."
Van Damme P., Lasa M., Polevoda B., Gazquez C., Elosegui-Artola A., Kim D.S., De Juan-Pardo E., Demeyer K., Hole K., Larrea E., Timmerman E., Prieto J., Arnesen T., Sherman F., Gevaert K., Aldabe R.
Proc. Natl. Acad. Sci. U.S.A. 109:12449-12454(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[17]"Structure of the yeast RNA polymerase II holoenzyme: mediator conformation and polymerase interaction."
Davis J.A., Takagi Y., Kornberg R.D., Asturias F.J.
Mol. Cell 10:409-415(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: ELECTRON MICROSCOPY OF MEDIATOR COMPLEX IN COMPLEX WITH RNA POLYMERASE II.
[18]"Head module control of mediator interactions."
Takagi Y., Calero G., Komori H., Brown J.A., Ehrensberger A.H., Hudmon A., Asturias F.J., Kornberg R.D.
Mol. Cell 23:355-364(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: ELECTRON MICROSCOPY OF THE MEDIATOR COMPLEX HEAD MODULE, FUNCTION OF THE MEDIATOR COMPLEX HEAD MODULE, INTERACTION OF THE MEDIATOR COMPLEX HEAD MODULE WITH RNA POLYMERASE II AND TFIIF, INTERACTION WITH MED8; SRB4 AND SRB5.
[19]"Structure and TBP binding of the Mediator head subcomplex Med8-Med18-Med20."
Lariviere L., Geiger S., Hoeppner S., Roether S., Straesser K., Cramer P.
Nat. Struct. Mol. Biol. 13:895-901(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (2.7 ANGSTROMS) OF 2-210 IN COMPLEX WITH MED8 AND SRB5, X-RAY CRYSTALLOGRAPHY (2.4 ANGSTROMS) IN COMPLEX WITH SRB5.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
S36754 Genomic DNA. Translation: AAB22223.1.
U00062 Genomic DNA. Translation: AAB68920.1.
BK006934 Genomic DNA. Translation: DAA06733.1.
PIRA38105.
RefSeqNP_011907.1. NM_001179171.1.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
2HZMX-ray2.40A/C/E/G1-210[»]
2HZSX-ray2.70A/C/E/G2-210[»]
3J1Oelectron microscopy16.00M1-210[»]
3RJ1X-ray4.30F/M/T1-210[»]
4GWPX-ray4.20F1-210[»]
4GWQX-ray4.50F1-210[»]
ProteinModelPortalP34162.
SMRP34162. Positions 2-210.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid36473. 304 interactions.
DIPDIP-107N.
IntActP34162. 18 interactions.
MINTMINT-473266.
STRING4932.YHR041C.

Proteomic databases

PaxDbP34162.
PeptideAtlasP34162.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYHR041C; YHR041C; YHR041C.
GeneID856437.
KEGGsce:YHR041C.

Organism-specific databases

CYGDYHR041c.
SGDS000001083. SRB2.

Phylogenomic databases

eggNOGNOG41765.
HOGENOMHOG000113535.
OMAFKIFRNN.
OrthoDBEOG7GTTFC.

Enzyme and pathway databases

BioCycYEAST:G3O-31100-MONOMER.

Gene expression databases

GenevestigatorP34162.

Family and domain databases

InterProIPR013921. Mediator_Med20.
IPR016532. Mediator_Med20_fun.
[Graphical view]
PfamPF08612. Med20. 1 hit.
[Graphical view]
PIRSFPIRSF007945. SRB2. 1 hit.
ProtoNetSearch...

Other

EvolutionaryTraceP34162.
NextBio982039.

Entry information

Entry nameMED20_YEAST
AccessionPrimary (citable) accession number: P34162
Secondary accession number(s): D3DKY9
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: February 1, 1994
Last modified: April 16, 2014
This is version 120 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome VIII

Yeast (Saccharomyces cerevisiae) chromosome VIII: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references