Homeobox protein YOX1 - Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

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P34161 (YOX1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified May 29, 2013. Version 110. History...

Clusters with 100%, 90%, 50% identity |

Documents (3) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Homeobox protein YOX1

Gene names

Name:	YOX1
Ordered Locus Names:	YML027W

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces ›

Protein attributes

Sequence length	385 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Transcriptional repressor required to restrict transcription of ECB-dependent genes to the G1/M phase by repressing their transcription during the interval from late G1 to M phases. Genes that contain a ECB (early cell box) element in their transcription regulatory region are transcribed only during G1/M phases. In vitro, is capable of binding to the DNA of the leucine tRNA gene. Ref.5
Subunit structure	Interacts with MCM1. Ref.5
Subcellular location	Nucleus.
Developmental stage	Expressed from late G1 phase. Ref.5
Induction	Transcriptionally activated by SWI4-SWI6 heterodimer.
Miscellaneous	Present with 861 molecules/cell in log phase SD medium.
Sequence similarities	Contains 1 homeobox DNA-binding domain.

Ontologies

Keywords
Biological process	Cell cycle Transcription Transcription regulation
Cellular component	Nucleus
Domain	Homeobox
Ligand	DNA-binding
Molecular function	Repressor
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	negative regulation of transcription involved in G1 phase of mitotic cell cycle Inferred from mutant phenotype Ref.5 PubMed 17314407. Source: SGD
Cellular_component	cytoplasm Inferred from direct assay PubMed 22842922. Source: SGD nuclear chromatin Inferred from direct assay PubMed 12464632. Source: SGD
Molecular_function	RNA polymerase II regulatory region sequence-specific DNA binding Inferred from direct assay Ref.5 PubMed 20385087. Source: SGD RNA polymerase II transcription factor binding transcription factor activity involved in negative regulation of transcription Inferred from mutant phenotype PubMed 20385087. Source: SGD sequence-specific DNA binding transcription factor activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 385

385

Homeobox protein YOX1

PRO_0000049387

Regions

DNA binding

176 – 235

Homeobox Ref.5

Amino acid modifications

Modified residue

358

Phosphoserine Ref.7

Experimental info

Sequence conflict

291 – 296

PRPHHS → QGLIIP Ref.1

Sequences

Sequence

Length

Mass (Da)

Tools

P34161 [UniParc].

Last modified October 1, 1996. Version 2.
Checksum: F21A295CDD97A282
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FASTA

385

42,739

        10         20         30         40         50         60 
MSQETKMLPS LSSLLSGTEI SSSPVSPSFT NPRTSFHLDD RGTIKLPPLN TSINRPRSVE 

        70         80         90        100        110        120 
SALRHTVTSL HENSSAYGDD MLKHTQSDSA LSSQLNSSQE TVDESHENLL LTPLNSKKRD 

       130        140        150        160        170        180 
YSVSSKKNDI LTPLSAAKSI IIPSASKEKR RAFAFITHSQ ETFPKKEPKI DNAPLARRKR 

       190        200        210        220        230        240 
RRTSSQELSI LQAEFEKCPA PSKEKRIELA ESCHMTEKAV QIWFQNKRQA VKRQRIATSK 

       250        260        270        280        290        300 
STTIIQTVSP PSPPLDVHAT PLASRVKADI LRDGSSCSRS SSSSPLENTP PRPHHSLNRR 

       310        320        330        340        350        360 
SSTPSIKRSQ ALTFHLNPQK KTLTPVKTSP NSRVNKLINS IDHSPSKAKR PVSNPSGSPK 

       370        380 
RKRKFGFKIV DQQPLKDLDP NAFRG

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XIII." Bowman S., Churcher C.M., Badcock K., Brown D., Chillingworth T., Connor R., Dedman K., Devlin K., Gentles S., Hamlin N., Hunt S., Jagels K., Lye G., Moule S., Odell C., Pearson D., Rajandream M.A., Rice P. Barrell B.G. Nature 387:90-93(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[2]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[3]	"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae." Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. LaBaer J. Genome Res. 17:536-543(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[4]	"In vitro binding to the leucine tRNA gene identifies a novel yeast homeobox gene." Kaufmann E. Chromosoma 102:174-179(1993) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-296.
[5]	"Conserved homeodomain proteins interact with MADS box protein Mcm1 to restrict ECB-dependent transcription to the M/G1 phase of the cell cycle." Pramila T., Miles S., GuhaThakurta D., Jemiolo D., Breeden L.L. Genes Dev. 16:3034-3045(2002) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, DNA-BINDING, DEVELOPMENTAL STAGE, INTERACTION WITH MCM1.
[6]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[7]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-358, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Z46659 Genomic DNA. Translation: CAA86628.1. AY693100 Genomic DNA. Translation: AAT93119.1. X62392 Genomic DNA. Translation: CAA44264.1. BK006946 Genomic DNA. Translation: DAA09871.1.
PIR	S49752.
RefSeq	NP_013685.1. NM_001182385.1.
3D structure databases
ProteinModelPortal	P34161.
SMR	P34161. Positions 177-236.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-4566N.
IntAct	P34161. 7 interactions.
MINT	MINT-557333.
STRING	4932.YML027W.
Proteomic databases
PaxDb	P34161.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YML027W; YML027W; YML027W.
GeneID	854981.
KEGG	sce:YML027W.
Organism-specific databases
CYGD	YML027w.
SGD	S000004489. YOX1.
Phylogenomic databases
eggNOG	COG5576.
GeneTree	ENSGT00530000068906.
HOGENOM	HOG000000747.
KO	K12411.
OrthoDB	EOG4NKG55.
Enzyme and pathway databases
BioCyc	YEAST:G3O-32629-MONOMER.
Gene expression databases
Genevestigator	P34161.
GermOnline	YML027W. Saccharomyces cerevisiae.
Family and domain databases
Gene3D	1.10.10.60. 1 hit.
InterPro	IPR017970. Homeobox_CS. IPR001356. Homeodomain. IPR009057. Homeodomain-like. [Graphical view]
Pfam	PF00046. Homeobox. 1 hit. [Graphical view]
SMART	SM00389. HOX. 1 hit. [Graphical view]
SUPFAM	SSF46689. Homeodomain_like. 1 hit.
PROSITE	PS00027. HOMEOBOX_1. 1 hit. PS50071. HOMEOBOX_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	978100.

Entry information

Entry name

YOX1_YEAST

Accession

Primary (citable) accession number: P34161
Secondary accession number(s): D6VZE7

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1994
Last sequence update:	October 1, 1996
Last modified:	May 29, 2013
This is version 110 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program

Relevant documents

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

Yeast chromosome XIII

Yeast (Saccharomyces cerevisiae) chromosome XIII: entries and gene names

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Amino acid modifications

Experimental info

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents