Reviewed, UniProtKB/Swiss-Prot P34156 (COG4_CHIOP)

Names and origin

Protein names	Recommended name:     Collagenolytic protease 23 kDa     EC=3.4.24.7
Organism	Chionoecetes opilio (Crab-beetle)
Taxonomic identifier	41210 [NCBI]
Taxonomic lineage	Eukaryota › Metazoa › Arthropoda › Crustacea › Malacostraca › Eumalacostraca › Eucarida › Decapoda › Pleocyemata › Brachyura › Eubrachyura › Majoidea › Majidae › Chionoecetes

Protein attributes

Sequence length	20 AA.
Sequence status	Fragment.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	This enzyme is a metal protease capable of degrading the native triple helix of collagen.
Catalytic activity	Cleavage of the triple helix of collagen at about three-quarters of the length of the molecule from the N-terminus, at 775-Gly-|-Ile-776 in the alpha-1(I) chain. Cleaves synthetic substrates and alpha-macroglobulins at bonds where P1' is a hydrophobic residue.
Cofactor	Binds 1 zinc ion per subunit By similarity.
Sequence similarities	Belongs to the peptidase M12A family.

Ontologies

Keywords
Biological process	Collagen degradation
Ligand	Zinc
Molecular function	Hydrolase Metalloprotease Protease
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	collagen catabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	metallopeptidase activity Inferred from electronic annotation. Source: UniProtKB-KW zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – ›20	›20	Collagenolytic protease 23 kDa		PRO_0000078184
Experimental info
	Non-terminal residue	20	1

Sequences

	Sequence		Length	Mass (Da)
	P34156-1 [UniParc]. Last modified February 1, 1994. Version 1. Checksum: 2BC7A93D022A97D8	FASTA	20	2,110
	10 20 AAILQDEYLX SGGVVPYVFG

References

[1]	"Isolation and characteristics of collagenolytic enzymes from the hepatopancreas of the crab Chionoecetes opilio." Klimova O.A., Vedishcheva Y.V., Strongin A.Y. Dokl. Akad. Nauk SSSR 317:482-484(1991) [PubMed: 1663026] [Abstract] Cited for: PROTEIN SEQUENCE. Tissue: Hepatopancreas.

Cross-references

3D structure databases
HSSP	HSSP built from PDB template 1IAE based on UniProtKB P07584.
ModBase	Search...
Enzyme and pathway databases
BRENDA	3.4.24.7. 299542.
Family and domain databases
InterPro	IPR006025. Pept_M_Zn_BS. [Graphical view]
PROSITE	PS00142. ZINC_PROTEASE. Partial match. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

COG4_CHIOP

Accession

Primary (citable) accession number: P34156

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1994
Last sequence update:	February 1, 1994
Last modified:	June 16, 2009
This is version 43 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)