P34156 (COG4_CHIOP) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names	Recommended name: Collagenolytic protease 23 kDa EC=3.4.24.7
Organism	Chionoecetes opilio (Crab-beetle)
Taxonomic identifier	41210 [NCBI]
Taxonomic lineage	Eukaryota › Metazoa › Arthropoda › Crustacea › Malacostraca › Eumalacostraca › Eucarida › Decapoda › Pleocyemata › Brachyura › Eubrachyura › Majoidea › Majidae › Chionoecetes

Protein attributes

Sequence length	20 AA.
Sequence status	Fragment.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	This enzyme is a metal protease capable of degrading the native triple helix of collagen.
Catalytic activity	Cleavage of the triple helix of collagen at about three-quarters of the length of the molecule from the N-terminus, at 775-Gly-|-Ile-776 in the alpha-1(I) chain. Cleaves synthetic substrates and alpha-macroglobulins at bonds where P1' is a hydrophobic residue.
Cofactor	Binds 1 zinc ion per subunit By similarity.
Sequence similarities	Belongs to the peptidase M12A family.

Ontologies

Keywords
Biological process	Collagen degradation
Ligand	Zinc
Molecular function	Hydrolase Metalloprotease Protease
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	collagen catabolic process Inferred from electronic annotation. Source: UniProtKB-KW proteolysis Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	metallopeptidase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – ›20	›20	Collagenolytic protease 23 kDa		PRO_0000078184
Experimental info
	Non-terminal residue	20	1

Sequences

	Sequence		Length	Mass (Da)
	P34156 [UniParc]. Last modified February 1, 1994. Version 1. Checksum: 2BC7A93D022A97D8	FASTA	20	2,110
	10 20 AAILQDEYLX SGGVVPYVFG

References

[1]	"Isolation and characteristics of collagenolytic enzymes from the hepatopancreas of the crab Chionoecetes opilio." Klimova O.A., Vedishcheva Y.V., Strongin A.Y. Dokl. Akad. Nauk SSSR 317:482-484(1991) [PubMed: 1663026] [Abstract] Cited for: PROTEIN SEQUENCE. Tissue: Hepatopancreas.

Cross-references

3D structure databases
ModBase	Search...
Protein family/group databases
MEROPS	M12.001.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
PROSITE	PS00142. ZINC_PROTEASE. Partial match. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

COG4_CHIOP

Accession

Primary (citable) accession number: P34156

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1994
Last sequence update:	February 1, 1994
Last modified:	November 16, 2011
This is version 48 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)