Reviewed, UniProtKB/Swiss-Prot P34154 (COG2_CHIOP)

Names and origin

Protein names	Recommended name:     Collagenolytic protease 35 kDa 2     EC=3.4.21.32 Alternative name(s):     Collagenolytic protease 35 kDa II
Organism	Chionoecetes opilio (Crab-beetle)
Taxonomic identifier	41210 [NCBI]
Taxonomic lineage	Eukaryota › Metazoa › Arthropoda › Crustacea › Malacostraca › Eumalacostraca › Eucarida › Decapoda › Pleocyemata › Brachyura › Eubrachyura › Majoidea › Majidae › Chionoecetes

Protein attributes

Sequence length	20 AA.
Sequence status	Fragment.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	This enzyme is a serine protease capable of degrading the native triple helix of collagen.
Catalytic activity	Hydrolysis of proteins, with broad specificity for peptide bonds. Native collagen is cleaved about 75% of the length of the molecule from the N-terminus. Low activity on small molecule substrates of both trypsin and chymotrypsin.
Sequence similarities	Belongs to the peptidase S1 family. Contains 1 peptidase S1 domain.

Ontologies

Keywords
Biological process	Collagen degradation
Molecular function	Hydrolase Protease Serine protease
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	collagen catabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	serine-type peptidase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – ›20	›20	Collagenolytic protease 35 kDa 2		PRO_0000088671
Regions
	Domain	1 – ›20	›20	Peptidase S1
Experimental info
	Non-terminal residue	20	1

Sequences

	Sequence		Length	Mass (Da)
	P34154-1 [UniParc]. Last modified February 1, 1994. Version 1. Checksum: 45A17C921B801016	FASTA	20	2,150
	10 20 IVGGTEVTPG EIPYQLSFQD

References

[1]	"Isolation and characteristics of collagenolytic enzymes from the hepatopancreas of the crab Chionoecetes opilio." Klimova O.A., Vedishcheva Y.V., Strongin A.Y. Dokl. Akad. Nauk SSSR 317:482-484(1991) [PubMed: 1663026] [Abstract] Cited for: PROTEIN SEQUENCE. Tissue: Hepatopancreas.

Cross-references

3D structure databases
ModBase	Search...
Enzyme and pathway databases
BRENDA	3.4.21.32. 299542.
Family and domain databases
InterPro	IPR018114. Peptidase_S1/S6_AS. [Graphical view]
PROSITE	PS50240. TRYPSIN_DOM. Partial match. PS00134. TRYPSIN_HIS. Partial match. PS00135. TRYPSIN_SER. Partial match. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

COG2_CHIOP

Accession

Primary (citable) accession number: P34154

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1994
Last sequence update:	February 1, 1994
Last modified:	March 3, 2009
This is version 45 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)