P34137 (PTP1_DICDI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Tyrosine-protein phosphatase 1
EC=3.1.3.48

Alternative name(s):
Protein-tyrosine-phosphate phosphohydrolase 1

Gene names

Name:	ptpA1-1
Synonyms:	ptp1
ORF Names:	DDB_G0273097
AND
Name:	ptpA1-2
Synonyms:	ptp1
ORF Names:	DDB_G0273817

Organism

Dictyostelium discoideum (Slime mold) [Reference proteome]

Taxonomic identifier

44689 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Amoebozoa › Mycetozoa › Dictyosteliida › Dictyostelium

Protein attributes

Sequence length	522 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	May have a role in growth and in the early stages of development. Affects the timing of development.
Catalytic activity	Protein tyrosine phosphate + H2O = protein tyrosine + phosphate.
Subcellular location	Cytoplasm. Cell membrane Probable. Note: Might be tethered to the plasma membrane.
Tissue specificity	Expressed predominantly in anterior-like cells and to a lesser degree in prestalk cells.
Developmental stage	Expressed at a very low level in vegetative cells, induced by 4 hours, maximally expressed at the tight aggregate stage and through the remainder of development.
Miscellaneous	The PTPase domain is interrupted by a PTPase insert which shares no homologies with other PTPase proteins.
Sequence similarities	Belongs to the protein-tyrosine phosphatase family. Non-receptor class subfamily. Contains 1 tyrosine-protein phosphatase domain.
Caution	The gene for this protein is duplicated in strains AX3 and AX4. These strains contain a duplication of a segment of 750 kb of chromosome 2 compared to the corresponding sequence in strain AX2.

Ontologies

Keywords
Cellular component	Cell membrane Cytoplasm Membrane
Molecular function	Hydrolase Protein phosphatase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	peptidyl-tyrosine dephosphorylation Inferred from electronic annotation. Source: GOC
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	protein tyrosine phosphatase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 522	522	Tyrosine-protein phosphatase 1		PRO_0000094886
Regions
	Domain	97 – 471	375	Tyrosine-protein phosphatase
	Region	327 – 426	100	PTPase insert (Asn-rich)
	Compositional bias	1 – 114	114	Ser-rich
	Compositional bias	382 – 400	19	Poly-Asn
Sites
	Active site	310	1	Phosphocysteine intermediate By similarity
Experimental info
	Sequence conflict	423 – 424	2	DL → T in AAA33241. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P34137 [UniParc]. Last modified December 4, 2007. Version 2. Checksum: EFCC04A0F6AEB8BF	FASTA	522	59,554
	10 20 30 40 50 60 MGSVESSNQM NGSIENKTNK IDISVLRPLP TRSNSSISLS SSSHSSFSRM GSLGSLPTNS 70 80 90 100 110 120 GSSSPYYNNS SFDLVDEERI KSSIYNLKNH IKCIHKIKEE FRLLEESVGP SETSEGDKKH 130 140 150 160 170 180 NTSKNRYTNI LPVNHTRVQL KKIQDKEGSD YINANYIDGA YPKQFICTQG PLPNTIADFW 190 200 210 220 230 240 RMVWENRCRI IVMLSRESEN CRIKCDRYWP EQIGGEQFSI YGNGNEVFGT YSVELVEVIQ 250 260 270 280 290 300 DPEREIITRN IRLTFEGETR DITQYQYEGW PDHNIPDHTQ PFRQLLHSIT NRQNQIIPSS 310 320 330 340 350 360 DRNVPIIVHC SAGVGRTGTF CTAVIMMKKL DHYFKQLDAT PIDQVVDPFT HLPITEYQSD 370 380 390 400 410 420 NLDLKGLGYH FKSSIYNSNG INNNNNNNLN NNNNINNNSN GSNNTPQTEP NNEEDDDDAA 430 440 450 460 470 480 ESDLKYAIMD KYNSRIDFNL FSIVLKLREQ RPGMVQQLEQ YLFCYKTILD EIYHRLNCKL 490 500 510 520 GFSLPHVNNI NNYNNYSNTT TTTTSSLAST TIIHPSTNSK LN

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Analysis of a spatially regulated phosphotyrosine phosphatase identifies tyrosine phosphorylation as a key regulatory pathway in Dictyostelium." Howard P.K., Sefton B.M., Firtel R.A. Cell 71:637-647(1992) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Sequence and analysis of chromosome 2 of Dictyostelium discoideum." Gloeckner G., Eichinger L., Szafranski K., Pachebat J.A., Bankier A.T., Dear P.H., Lehmann R., Baumgart C., Parra G., Abril J.F., Guigo R., Kumpf K., Tunggal B., Cox E.C., Quail M.A., Platzer M., Rosenthal A., Noegel A.A. Nature 418:79-85(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: AX4.
[3]	"The genome of the social amoeba Dictyostelium discoideum." Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N. , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A. Nature 435:43-57(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: AX4.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	L07125 Genomic DNA. Translation: AAA33241.1. AAFI02000011 Genomic DNA. Translation: EAL70599.1. AAFI02000009 Genomic DNA. Translation: EAL70766.1.
PIR	A44267.
RefSeq	XP_644525.1. XM_639433.1. XP_644725.1. XM_639633.1.
3D structure databases
ProteinModelPortal	P34137.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblProtists	DDB0185117; DDB0185117; DDB_G0273097. DDB0238491; DDB0238491; DDB_G0273817.
GeneID	8618825. 8619151.
KEGG	ddi:DDB_G0273097. ddi:DDB_G0273817.
Organism-specific databases
dictyBase	DDB_G0273097. ptpA1-1. DDB_G0273817. ptpA1-2.
Phylogenomic databases
eggNOG	COG5599.
OMA	ENCRIKC.
ProtClustDB	CLSZ2431184.
Family and domain databases
InterPro	IPR000387. Tyr/Dual-sp_Pase. IPR016130. Tyr_Pase_AS. IPR000242. Tyr_Pase_rcpt/non-rcpt. [Graphical view]
Pfam	PF00102. Y_phosphatase. 2 hits. [Graphical view]
PRINTS	PR00700. PRTYPHPHTASE.
SMART	SM00194. PTPc. 1 hit. [Graphical view]
PROSITE	PS00383. TYR_PHOSPHATASE_1. 1 hit. PS50056. TYR_PHOSPHATASE_2. 1 hit. PS50055. TYR_PHOSPHATASE_PTP. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PTP1_DICDI

Accession

Primary (citable) accession number: P34137
Secondary accession number(s): Q556U4, Q86AJ9

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1994
Last sequence update:	December 4, 2007
Last modified:	May 1, 2013
This is version 89 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)