P34135 (HMDH1_DICDI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
3-hydroxy-3-methylglutaryl-coenzyme A reductase 1
Short name=HMG-CoA reductase 1
EC=1.1.1.34

Gene names

Name:	hmgA
ORF Names:	DDB_G0269142

Organism

Dictyostelium discoideum (Slime mold)

Taxonomic identifier

44689 [NCBI]

Taxonomic lineage

Eukaryota › Amoebozoa › Mycetozoa › Dictyosteliida › Dictyostelium

Protein attributes

Sequence length	552 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	This transmembrane glycoprotein is involved in the control of cholesterol biosynthesis. It is the rate-limiting enzyme of the sterol biosynthesis.
Catalytic activity	(R)-mevalonate + CoA + 2 NADP+ = (S)-3-hydroxy-3-methylglutaryl-CoA + 2 NADPH.
Pathway	Metabolic intermediate biosynthesis; (R)-mevalonate biosynthesis; (R)-mevalonate from acetyl-CoA: step 3/3.
Subcellular location	Endoplasmic reticulum membrane.
Sequence similarities	Belongs to the HMG-CoA reductase family.

Ontologies

Keywords
Biological process	Cholesterol biosynthesis Lipid synthesis Steroid biosynthesis Sterol biosynthesis
Cellular component	Endoplasmic reticulum Membrane
Ligand	NADP
Molecular function	Oxidoreductase
PTM	Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	cholesterol biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW coenzyme A metabolic process Inferred from electronic annotation. Source: InterPro isoprenoid biosynthetic process Inferred from electronic annotation. Source: InterPro
Cellular component	endoplasmic reticulum membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: InterPro
Molecular function	NADP binding Inferred from electronic annotation. Source: InterPro hydroxymethylglutaryl-CoA reductase (NADPH) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 552	552	3-hydroxy-3-methylglutaryl-coenzyme A reductase 1		PRO_0000114427
Sites
	Active site	237	1	Charge relay system By similarity
	Active site	369	1	Charge relay system By similarity
	Active site	445	1	Charge relay system By similarity
	Active site	543	1	Proton donor By similarity
Amino acid modifications
	Glycosylation	288	1	N-linked (GlcNAc...) Potential
	Glycosylation	375	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	P34135 [UniParc]. Last modified February 1, 1994. Version 1. Checksum: 3535CF268CDCE693	FASTA	552	60,387
	10 20 30 40 50 60 MLFAPPNLET KELFWIIYIL ILIPKVFAKV MSVRELFPFF KWGFNIRRSN FLVPILSNNV 70 80 90 100 110 120 IVTGEEAVQY EKPLPYIPQH NQQQQQKQQP SQDYIQQPQN DNNINSGKEQ EQQQQQQQQQ 130 140 150 160 170 180 QQTPDITNQP TKTNKKIPIK ELSNEEILIK LEKGEVLAYR LENELGDCSR AVEIRRMLLE 190 200 210 220 230 240 KQLSKKIEPI PHEGFDFAKV QGQCCENVIG YVPIPVGTAG PIQLNGQLVT IPMATTEGCL 250 260 270 280 290 300 VASTHRGCKA ITESGGAKCT ITSRGMTRAP VVRFSDIVKA SEFVSWINDT DNYQALKAVF 310 320 330 340 350 360 DSTSRFARLS AIKCTIAGRS VYIRFKCDTG DAMGMNMVSK GVEAVLEHLK IIFDDMTLLS 370 380 390 400 410 420 ISGNMCTDKK PSSINWTEGR GRSVVCEAMI TGDVVQRVLK TNVQALVDLN IAKNLIGSAM 430 440 450 460 470 480 AGSIGGFNAH ASNIVTAIFL ATGQDCAQNV ESSNCITQME ACNDGQDLYI TVTMPSIEVG 490 500 510 520 530 540 TVGGGTSLPA QSACLDIIGV KGSSSSKPGA NADQLAKTIA SAVMAGELSL MSALSAGHLM 550 KSHLQYNRAK TN

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The dictyostelium HMGCoA reductase genes." De Lozanne A. Submitted (JUN-1993) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: AX3.
[2]	"The genome of the social amoeba Dictyostelium discoideum." Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N. , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A. Nature 435:43-57(2005) [PubMed: 15875012] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: AX4.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	L19349 mRNA. Translation: AAA33214.1. AAFI02000005 Genomic DNA. Translation: EAL71921.1.
RefSeq	XP_646489.1. XM_641397.1.
3D structure databases
ProteinModelPortal	P34135.
SMR	P34135. Positions 130-540.
ModBase	Search...
Protein-protein interaction databases
STRING	P34135.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblProtists	DDB0191125; DDB0191125; DDB_G0269142.
GeneID	8617451.
GenomeReviews	Gene locus hmgA in contig CM000150_GR.
KEGG	ddi:DDB_G0269142.
Organism-specific databases
dictyBase	DDB_G0269142. hmgA.
Phylogenomic databases
eggNOG	KOG2480.
GeneTree	EPrGT00050000005797.
HOGENOM	HBG604528.
OMA	ASEEDEM.
ProtClustDB	CLSZ2430888.
Family and domain databases
InterPro	IPR002202. HMG_CoA_Rdtase. IPR023074. HMG_CoA_Rdtase_cat. IPR023076. HMG_CoA_Rdtase_CS. IPR004554. HMG_CoA_Rdtase_eu_arc. IPR023282. HMG_CoA_Rdtase_N. IPR009023. HMG_CoA_Rdtase_NAD(P)-bd. IPR009029. HMG_CoA_Rdtase_sub-bd. [Graphical view]
Gene3D	G3DSA:3.30.70.420. G3DSA:3.30.70.420. 1 hit. G3DSA:3.90.770.10. HMG-CoA_red. 2 hits. G3DSA:1.10.3270.10. HMG_CoA_Rdtase_N. 1 hit.
KO	K00021.
PANTHER	PTHR10572. HMG-CoA_red. 1 hit.
Pfam	PF00368. HMG-CoA_red. 1 hit. [Graphical view]
PRINTS	PR00071. HMGCOARDTASE.
SUPFAM	SSF55035. HMG_CoA_NAD_bind. 1 hit. SSF56542. HMG_CoA_sub_bind. 1 hit.
TIGRFAMs	TIGR00533. HMG_CoA_R_NADP. 1 hit.
PROSITE	PS00066. HMG_COA_REDUCTASE_1. 1 hit. PS00318. HMG_COA_REDUCTASE_2. 1 hit. PS01192. HMG_COA_REDUCTASE_3. 1 hit. PS50065. HMG_COA_REDUCTASE_4. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

HMDH1_DICDI

Accession

Primary (citable) accession number: P34135
Secondary accession number(s): Q55CJ2

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1994
Last sequence update:	February 1, 1994
Last modified:	November 16, 2011
This is version 82 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)