Diacylglycerol kinase A - Dictyostelium discoideum (Slime mold)

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P34125 (DGKA_DICDI) Reviewed, UniProtKB/Swiss-Prot

Last modified November 16, 2011. Version 95. History...

Clusters with 100%, 90%, 50% identity |

Documents (2) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Diacylglycerol kinase A
EC=2.7.1.107

Alternative name(s):
Myosin heavy chain kinase
Short name=MHCK

Gene names

Name:	dgkA
Synonyms:	mhcK
ORF Names:	DDB_G0277223

Organism

Dictyostelium discoideum (Slime mold)

Taxonomic identifier

44689 [NCBI]

Taxonomic lineage

Eukaryota › Amoebozoa › Mycetozoa › Dictyosteliida › Dictyostelium

Protein attributes

Sequence length	887 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Catalytic activity	ATP + 1,2-diacylglycerol = ADP + 1,2-diacyl-sn-glycerol 3-phosphate. Ref.1 Ref.5 Ref.6
Subcellular location	Membrane; Peripheral membrane protein Ref.4.
Developmental stage	Expressed at low level during vegetative growth and during early development. Higher levels of expression are detected later during development (12-24 hours). Ref.1 Ref.4
Sequence similarities	Belongs to the eukaryotic diacylglycerol kinase family. Contains 1 DAGKc domain. Contains 3 phorbol-ester/DAG-type zinc fingers.
Caution	The initially described myosin heavy chain kinase activity is probably artifactual due to numerous errors in the sequence used for characterization (Ref.4).
Biophysicochemical properties	Kinetic parameters: K_M=148 µM for ATP (in the presence of 1,2-dioctanoyl-sn-glycerol) Ref.5 Ref.6 K_M=336 µM for ATP (in the presence of 1,2-dihexanoyl-sn-glycerol) V_max=40 nmol/min/mg enzyme towards ATP (in the presence of 1,2-dioctanoyl-sn-glycerol) V_max=100 nmol/min/mg enzyme towards ATP (in the presence of 1,2-dihexanoyl-sn-glycerol) pH dependence: Optimum pH is 7.0 with 1,2-dioctanoyl-sn-glycerol as a substrate, and 7.4 with 1,2-dihexanoyl-sn-glycerol as a substrate.

Ontologies

Keywords
Cellular component	Membrane
Domain	Repeat Zinc-finger
Ligand	ATP-binding Metal-binding Nucleotide-binding Zinc
Molecular function	Kinase Serine/threonine-protein kinase Transferase
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	activation of protein kinase C activity by G-protein coupled receptor protein signaling pathway Inferred from direct assay Ref.6. Source: UniProtKB anatomical structure morphogenesis Inferred from mutant phenotype. Source: dictyBase intracellular signal transduction Inferred from direct assay Ref.6. Source: UniProtKB protein autophosphorylation Inferred from direct assay. Source: dictyBase regulation of positive chemotaxis Inferred from mutant phenotype. Source: dictyBase
Cellular component	insoluble fraction Inferred from direct assay. Source: dictyBase intracellular Inferred from direct assay Ref.6. Source: GOC membrane Inferred from direct assay Ref.4. Source: UniProtKB soluble fraction Inferred from direct assay. Source: dictyBase
Molecular function	ATP binding Inferred from direct assay Ref.5. Source: UniProtKB diacylglycerol binding Inferred from direct assay Ref.5. Source: UniProtKB diacylglycerol kinase activity Inferred from direct assay Ref.6. Source: UniProtKB metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW protein serine/threonine kinase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 887

887

Diacylglycerol kinase A

PRO_0000218476

Regions

Domain

330 – 473

144

DAGKc

Zinc finger

7 – 57

Phorbol-ester/DAG-type 1

Zinc finger

140 – 190

Phorbol-ester/DAG-type 2

Zinc finger

208 – 259

Phorbol-ester/DAG-type 3

Compositional bias

72 – 116

Thr-rich

Compositional bias

309 – 317

Poly-Asn

Compositional bias

720 – 746

Gln-rich

Compositional bias

752 – 804

Poly-Asn

Compositional bias

827 – 838

Poly-Asn

Compositional bias

850 – 871

Poly-Asn

Experimental info

Sequence conflict

828

D → E in AAN39880. Ref.1

Sequences

Sequence

Length

Mass (Da)

Tools

P34125 [UniParc].

Last modified December 4, 2007. Version 3.
Checksum: B93209A9876B6132
Show »

FASTA

887

99,177

        10         20         30         40         50         60 
MNQLNISHNW KIKSFTSITF CDHCGSMLWG ICTQGFQCSD CNFSAHSHCT NLVTLHCNNN 

        70         80         90        100        110        120 
KNNSKPNEKP ATITAAAASS STTTTTTTET STNTAASTTT TTNNIDIKNK DKTPTTSPFS 

       130        140        150        160        170        180 
SPILSSYNNN SGNEFCSNRQ HKFHKTSKSI TKFCNYCRET TFTISGEPVM CSECRYIAHG 

       190        200        210        220        230        240 
HCQTKVPLNC IIPYSSPINT INDGGNVRHH WVEGNLKKSK KCIHCMEPCE KSFSLAHYKC 

       250        260        270        280        290        300 
LWCHKYLHSS CFDKHNPICD FGSLSDMILP PSSIRLLSTT ATTTNKIEKL IIEEQQPQLS 

       310        320        330        340        350        360 
SSPSSPRLNI NNNNNNNEII EWELIENSNM PEKVLFVFVN SKSGGQFGST LIRKLSSLLN 

       370        380        390        400        410        420 
PLQIIDLIKC GGPDSTLQMI NRYLAKHPEQ TNRFRILVCG GDGTVGWLFK QMTKHLVPST 

       430        440        450        460        470        480 
IPIGIIPLGT GNDLARSLGW GIGYDGEKLI EILKSINEAK TIQMDTWSIE MWDDDKPEDR 

       490        500        510        520        530        540 
RVIEMNNYFS IGLDAMVALG FHLARNANPQ LFTGRTVNKL WYTKIGLEEF VTKNFVSLAR 

       550        560        570        580        590        600 
IVKINVGTRE IRVDRSIEGI IILNLGSYAG GVDLWGANRK DNQTHSDGTG NQFIDDQTLE 

       610        620        630        640        650        660 
IVGVTSLPHL GSCLSSISSP IKMGQADEIR IQVSMPSIIL KDKSNEIETA FQVDGEPEPI 

       670        680        690        700        710        720 
EVRNCTFKIS FFKKVSMLSN RSFKPRIENH DSDLSTFQIS QSPNLYSPPS FKNPPPNLNQ 

       730        740        750        760        770        780 
YQQPHQPPQS QPQPQPQPQQ SLQSPQSPEP INNNTNNNNN NNNNNNNNNN NNNNNNNNNN 

       790        800        810        820        830        840 
NNNNNNNNNN NNNNNNNSNN NNNNISNDLT NNSEVNPEKS TNNEKTNDNN NNNNNNINTP 

       850        860        870        880 
FEPISLPDEN NNNNNNNNNN NNNNNNNNNN NETNSEKSYT NNCDEVD

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Dictyostelium discoideum has a single diacylglycerol kinase gene with similarity to mammalian theta isoforms." De La Roche M.A., Smith J.L., Rico M., Carrasco S., Merida I., Licate L., Cote G.P., Egelhoff T.T. Biochem. J. 368:809-815(2002) [PubMed: 12296770] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], CATALYTIC ACTIVITY, DEVELOPMENTAL STAGE. Strain: AX2.
[2]	"Sequence and analysis of chromosome 2 of Dictyostelium discoideum." Gloeckner G., Eichinger L., Szafranski K., Pachebat J.A., Bankier A.T., Dear P.H., Lehmann R., Baumgart C., Parra G., Abril J.F., Guigo R., Kumpf K., Tunggal B., Cox E.C., Quail M.A., Platzer M., Rosenthal A., Noegel A.A. Nature 418:79-85(2002) [PubMed: 12097910] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: AX4.
[3]	"The genome of the social amoeba Dictyostelium discoideum." Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N. Kuspa A. Nature 435:43-57(2005) [PubMed: 15875012] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: AX4.
[4]	"Membrane-bound Dictyostelium myosin heavy chain kinase: a developmentally regulated substrate-specific member of the protein kinase C family." Ravid S., Spudich J.A. Proc. Natl. Acad. Sci. U.S.A. 89:5877-5881(1992) [PubMed: 1321427] [Abstract] Cited for: PRELIMINARY PARTIAL NUCLEOTIDE SEQUENCE [MRNA], SUBCELLULAR LOCATION, DEVELOPMENTAL STAGE. Strain: AX3.
[5]	"Analysis of a novel diacylglycerol kinase from Dictyostelium discoideum: DGKA." Ostroski M., Tu-Sekine B., Raben D.M. Biochemistry 44:10199-10207(2005) [PubMed: 16042397] [Abstract] Cited for: CATALYTIC ACTIVITY, BIOPHYSICOCHEMICAL PROPERTIES.
[6]	"Analysis of two diacylglycerol kinase activities in mixed micelles." Tu-Sekine B., Ostroski M., Raben D.M. Adv. Enzyme Regul. 46:12-24(2006) [PubMed: 16854454] [Abstract] Cited for: CATALYTIC ACTIVITY, BIOPHYSICOCHEMICAL PROPERTIES.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AY152858 Genomic DNA. Translation: AAN39880.1. AAFI02000019 Genomic DNA. Translation: EAL68797.1. M93393 mRNA. No translation available.
PIR	A46136.
RefSeq	XP_642726.1. XM_637634.1.
3D structure databases
ProteinModelPortal	P34125.
ModBase	Search...
Protein-protein interaction databases
STRING	P34125.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblProtists	DDB0185037; DDB0185037; DDB_G0277223.
GeneID	8620920.
GenomeReviews	Gene locus dgkA in contig CM000151_GR.
KEGG	ddi:DDB_G0277223.
Organism-specific databases
dictyBase	DDB_G0277223. dgkA.
Phylogenomic databases
eggNOG	KOG1169.
GeneTree	EPrGT00060000009578.
PhylomeDB	P34125.
Enzyme and pathway databases
BRENDA	2.7.11.7. 1939.
Family and domain databases
InterPro	IPR000756. Diacylglycerol_kin_accessory. IPR001206. Diacylglycerol_kinase_cat_dom. IPR002219. Prot_Kinase_C-like_PE/DAG-bd. [Graphical view]
KO	K00901.
Pfam	PF00130. C1_1. 3 hits. PF00609. DAGK_acc. 1 hit. PF00781. DAGK_cat. 1 hit. [Graphical view]
SMART	SM00109. C1. 3 hits. SM00045. DAGKa. 1 hit. SM00046. DAGKc. 1 hit. [Graphical view]
PROSITE	PS50146. DAGK. 1 hit. PS00479. ZF_DAG_PE_1. 2 hits. PS50081. ZF_DAG_PE_2. 3 hits. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DGKA_DICDI

Accession

Primary (citable) accession number: P34125
Secondary accession number(s): Q54ZZ0, Q86AQ4, Q8I8F4

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1994
Last sequence update:	December 4, 2007
Last modified:	November 16, 2011
This is version 95 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Relevant documents

Dictyostelium discoideum

Dictyostelium discoideum: entries, gene names and cross-references to dictyBase

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Experimental info

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Family and domain databases

Entry information

Relevant documents