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P34125

- DGKA_DICDI

UniProt

P34125 - DGKA_DICDI

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Protein

Diacylglycerol kinase A

Gene

dgkA

Organism
Dictyostelium discoideum (Slime mold)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Catalytic activityi

ATP + 1,2-diacyl-sn-glycerol = ADP + 1,2-diacyl-sn-glycerol 3-phosphate.3 Publications

Kineticsi

  1. KM=148 µM for ATP (in the presence of 1,2-dioctanoyl-sn-glycerol)2 Publications
  2. KM=336 µM for ATP (in the presence of 1,2-dihexanoyl-sn-glycerol)2 Publications

Vmax=40 nmol/min/mg enzyme towards ATP (in the presence of 1,2-dioctanoyl-sn-glycerol)2 Publications

Vmax=100 nmol/min/mg enzyme towards ATP (in the presence of 1,2-dihexanoyl-sn-glycerol)2 Publications

pH dependencei

Optimum pH is 7.0 with 1,2-dioctanoyl-sn-glycerol as a substrate, and 7.4 with 1,2-dihexanoyl-sn-glycerol as a substrate.2 Publications

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri7 – 5751Phorbol-ester/DAG-type 1PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri140 – 19051Phorbol-ester/DAG-type 2PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri208 – 25952Phorbol-ester/DAG-type 3PROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

  1. ATP binding Source: UniProtKB
  2. diacylglycerol binding Source: UniProtKB
  3. diacylglycerol kinase activity Source: UniProtKB
  4. metal ion binding Source: UniProtKB-KW
  5. NAD+ kinase activity Source: InterPro
  6. nucleotide binding Source: UniProtKB
  7. protein kinase activity Source: dictyBase
  8. protein serine/threonine kinase activity Source: UniProtKB-KW

GO - Biological processi

  1. anatomical structure morphogenesis Source: dictyBase
  2. intracellular signal transduction Source: UniProtKB
  3. protein autophosphorylation Source: dictyBase
  4. protein kinase C-activating G-protein coupled receptor signaling pathway Source: UniProtKB
  5. regulation of positive chemotaxis Source: dictyBase
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BRENDAi2.7.11.7. 1939.
SABIO-RKP34125.

Names & Taxonomyi

Protein namesi
Recommended name:
Diacylglycerol kinase A (EC:2.7.1.107)
Alternative name(s):
Myosin heavy chain kinase
Short name:
MHCK
Gene namesi
Name:dgkA
Synonyms:mhcK
ORF Names:DDB_G0277223
OrganismiDictyostelium discoideum (Slime mold)
Taxonomic identifieri44689 [NCBI]
Taxonomic lineageiEukaryotaAmoebozoaMycetozoaDictyosteliidaDictyostelium
ProteomesiUP000002195: Chromosome 2, UP000002195: Unassembled WGS sequence

Organism-specific databases

dictyBaseiDDB_G0277223. dgkA.

Subcellular locationi

Membrane 1 Publication; Peripheral membrane protein 1 Publication

GO - Cellular componenti

  1. membrane Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 887887Diacylglycerol kinase APRO_0000218476Add
BLAST

Keywords - PTMi

Phosphoprotein

Expressioni

Developmental stagei

Expressed at low level during vegetative growth and during early development. Higher levels of expression are detected later during development (12-24 hours).2 Publications

Interactioni

Protein-protein interaction databases

STRINGi44689.DDB_0185037.

Structurei

3D structure databases

ProteinModelPortaliP34125.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini330 – 473144DAGKcPROSITE-ProRule annotationAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi72 – 11645Thr-richAdd
BLAST
Compositional biasi309 – 3179Poly-Asn
Compositional biasi720 – 74627Gln-richAdd
BLAST
Compositional biasi752 – 80453Poly-AsnAdd
BLAST
Compositional biasi827 – 83812Poly-AsnAdd
BLAST
Compositional biasi850 – 87122Poly-AsnAdd
BLAST

Sequence similaritiesi

Contains 1 DAGKc domain.PROSITE-ProRule annotation
Contains 3 phorbol-ester/DAG-type zinc fingers.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri7 – 5751Phorbol-ester/DAG-type 1PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri140 – 19051Phorbol-ester/DAG-type 2PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri208 – 25952Phorbol-ester/DAG-type 3PROSITE-ProRule annotationAdd
BLAST

Keywords - Domaini

Repeat, Zinc-finger

Phylogenomic databases

eggNOGiNOG47311.
InParanoidiP34125.
KOiK00901.
OMAiARNANPQ.
PhylomeDBiP34125.

Family and domain databases

InterProiIPR016064. ATP-NAD_kinase_PpnK-typ.
IPR000756. Diacylglycerol_kin_accessory.
IPR001206. Diacylglycerol_kinase_cat_dom.
IPR002219. PE/DAG-bd.
[Graphical view]
PfamiPF00130. C1_1. 3 hits.
PF00609. DAGK_acc. 1 hit.
PF00781. DAGK_cat. 1 hit.
[Graphical view]
SMARTiSM00109. C1. 3 hits.
SM00045. DAGKa. 1 hit.
SM00046. DAGKc. 1 hit.
[Graphical view]
SUPFAMiSSF111331. SSF111331. 1 hit.
PROSITEiPS50146. DAGK. 1 hit.
PS00479. ZF_DAG_PE_1. 2 hits.
PS50081. ZF_DAG_PE_2. 3 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P34125-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MNQLNISHNW KIKSFTSITF CDHCGSMLWG ICTQGFQCSD CNFSAHSHCT
60 70 80 90 100
NLVTLHCNNN KNNSKPNEKP ATITAAAASS STTTTTTTET STNTAASTTT
110 120 130 140 150
TTNNIDIKNK DKTPTTSPFS SPILSSYNNN SGNEFCSNRQ HKFHKTSKSI
160 170 180 190 200
TKFCNYCRET TFTISGEPVM CSECRYIAHG HCQTKVPLNC IIPYSSPINT
210 220 230 240 250
INDGGNVRHH WVEGNLKKSK KCIHCMEPCE KSFSLAHYKC LWCHKYLHSS
260 270 280 290 300
CFDKHNPICD FGSLSDMILP PSSIRLLSTT ATTTNKIEKL IIEEQQPQLS
310 320 330 340 350
SSPSSPRLNI NNNNNNNEII EWELIENSNM PEKVLFVFVN SKSGGQFGST
360 370 380 390 400
LIRKLSSLLN PLQIIDLIKC GGPDSTLQMI NRYLAKHPEQ TNRFRILVCG
410 420 430 440 450
GDGTVGWLFK QMTKHLVPST IPIGIIPLGT GNDLARSLGW GIGYDGEKLI
460 470 480 490 500
EILKSINEAK TIQMDTWSIE MWDDDKPEDR RVIEMNNYFS IGLDAMVALG
510 520 530 540 550
FHLARNANPQ LFTGRTVNKL WYTKIGLEEF VTKNFVSLAR IVKINVGTRE
560 570 580 590 600
IRVDRSIEGI IILNLGSYAG GVDLWGANRK DNQTHSDGTG NQFIDDQTLE
610 620 630 640 650
IVGVTSLPHL GSCLSSISSP IKMGQADEIR IQVSMPSIIL KDKSNEIETA
660 670 680 690 700
FQVDGEPEPI EVRNCTFKIS FFKKVSMLSN RSFKPRIENH DSDLSTFQIS
710 720 730 740 750
QSPNLYSPPS FKNPPPNLNQ YQQPHQPPQS QPQPQPQPQQ SLQSPQSPEP
760 770 780 790 800
INNNTNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNSNN
810 820 830 840 850
NNNNISNDLT NNSEVNPEKS TNNEKTNDNN NNNNNNINTP FEPISLPDEN
860 870 880
NNNNNNNNNN NNNNNNNNNN NETNSEKSYT NNCDEVD
Length:887
Mass (Da):99,177
Last modified:December 4, 2007 - v3
Checksum:iB93209A9876B6132
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti828 – 8281D → E in AAN39880. (PubMed:12296770)Curated

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AY152858 Genomic DNA. Translation: AAN39880.1.
AAFI02000019 Genomic DNA. Translation: EAL68797.1.
M93393 mRNA. No translation available.
PIRiA46136.
RefSeqiXP_642726.1. XM_637634.1.

Genome annotation databases

EnsemblProtistsiDDB0185037; DDB0185037; DDB_G0277223.
GeneIDi8620920.
KEGGiddi:DDB_G0277223.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AY152858 Genomic DNA. Translation: AAN39880.1 .
AAFI02000019 Genomic DNA. Translation: EAL68797.1 .
M93393 mRNA. No translation available.
PIRi A46136.
RefSeqi XP_642726.1. XM_637634.1.

3D structure databases

ProteinModelPortali P34125.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 44689.DDB_0185037.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblProtistsi DDB0185037 ; DDB0185037 ; DDB_G0277223 .
GeneIDi 8620920.
KEGGi ddi:DDB_G0277223.

Organism-specific databases

dictyBasei DDB_G0277223. dgkA.

Phylogenomic databases

eggNOGi NOG47311.
InParanoidi P34125.
KOi K00901.
OMAi ARNANPQ.
PhylomeDBi P34125.

Enzyme and pathway databases

BRENDAi 2.7.11.7. 1939.
SABIO-RK P34125.

Family and domain databases

InterProi IPR016064. ATP-NAD_kinase_PpnK-typ.
IPR000756. Diacylglycerol_kin_accessory.
IPR001206. Diacylglycerol_kinase_cat_dom.
IPR002219. PE/DAG-bd.
[Graphical view ]
Pfami PF00130. C1_1. 3 hits.
PF00609. DAGK_acc. 1 hit.
PF00781. DAGK_cat. 1 hit.
[Graphical view ]
SMARTi SM00109. C1. 3 hits.
SM00045. DAGKa. 1 hit.
SM00046. DAGKc. 1 hit.
[Graphical view ]
SUPFAMi SSF111331. SSF111331. 1 hit.
PROSITEi PS50146. DAGK. 1 hit.
PS00479. ZF_DAG_PE_1. 2 hits.
PS50081. ZF_DAG_PE_2. 3 hits.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Dictyostelium discoideum has a single diacylglycerol kinase gene with similarity to mammalian theta isoforms."
    De La Roche M.A., Smith J.L., Rico M., Carrasco S., Merida I., Licate L., Cote G.P., Egelhoff T.T.
    Biochem. J. 368:809-815(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], CATALYTIC ACTIVITY, DEVELOPMENTAL STAGE.
    Strain: AX2.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: AX4.
  3. "The genome of the social amoeba Dictyostelium discoideum."
    Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N.
    , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.
    Nature 435:43-57(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: AX4.
  4. "Membrane-bound Dictyostelium myosin heavy chain kinase: a developmentally regulated substrate-specific member of the protein kinase C family."
    Ravid S., Spudich J.A.
    Proc. Natl. Acad. Sci. U.S.A. 89:5877-5881(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: PRELIMINARY PARTIAL NUCLEOTIDE SEQUENCE [MRNA], SUBCELLULAR LOCATION, DEVELOPMENTAL STAGE.
    Strain: AX3.
  5. "Analysis of a novel diacylglycerol kinase from Dictyostelium discoideum: DGKA."
    Ostroski M., Tu-Sekine B., Raben D.M.
    Biochemistry 44:10199-10207(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: CATALYTIC ACTIVITY, BIOPHYSICOCHEMICAL PROPERTIES.
  6. "Analysis of two diacylglycerol kinase activities in mixed micelles."
    Tu-Sekine B., Ostroski M., Raben D.M.
    Adv. Enzyme Regul. 46:12-24(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: CATALYTIC ACTIVITY, BIOPHYSICOCHEMICAL PROPERTIES.

Entry informationi

Entry nameiDGKA_DICDI
AccessioniPrimary (citable) accession number: P34125
Secondary accession number(s): Q54ZZ0, Q86AQ4, Q8I8F4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: December 4, 2007
Last modified: October 29, 2014
This is version 111 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Caution

The initially described myosin heavy chain kinase activity is probably artifactual due to numerous errors in the sequence used for characterization.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Dictyostelium discoideum
    Dictyostelium discoideum: entries, gene names and cross-references to dictyBase
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3