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P34125

- DGKA_DICDI

UniProt

P34125 - DGKA_DICDI

Protein

Diacylglycerol kinase A

Gene

dgkA

Organism
Dictyostelium discoideum (Slime mold)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 110 (01 Oct 2014)
      Sequence version 3 (04 Dec 2007)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    ATP + 1,2-diacyl-sn-glycerol = ADP + 1,2-diacyl-sn-glycerol 3-phosphate.3 Publications

    Kineticsi

    1. KM=148 µM for ATP (in the presence of 1,2-dioctanoyl-sn-glycerol)2 Publications
    2. KM=336 µM for ATP (in the presence of 1,2-dihexanoyl-sn-glycerol)2 Publications

    Vmax=40 nmol/min/mg enzyme towards ATP (in the presence of 1,2-dioctanoyl-sn-glycerol)2 Publications

    Vmax=100 nmol/min/mg enzyme towards ATP (in the presence of 1,2-dihexanoyl-sn-glycerol)2 Publications

    pH dependencei

    Optimum pH is 7.0 with 1,2-dioctanoyl-sn-glycerol as a substrate, and 7.4 with 1,2-dihexanoyl-sn-glycerol as a substrate.2 Publications

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri7 – 5751Phorbol-ester/DAG-type 1PROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri140 – 19051Phorbol-ester/DAG-type 2PROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri208 – 25952Phorbol-ester/DAG-type 3PROSITE-ProRule annotationAdd
    BLAST

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB
    2. diacylglycerol binding Source: UniProtKB
    3. diacylglycerol kinase activity Source: UniProtKB
    4. metal ion binding Source: UniProtKB-KW
    5. NAD+ kinase activity Source: InterPro
    6. nucleotide binding Source: UniProtKB
    7. protein kinase activity Source: dictyBase
    8. protein serine/threonine kinase activity Source: UniProtKB-KW

    GO - Biological processi

    1. anatomical structure morphogenesis Source: dictyBase
    2. intracellular signal transduction Source: UniProtKB
    3. protein autophosphorylation Source: dictyBase
    4. protein kinase C-activating G-protein coupled receptor signaling pathway Source: UniProtKB
    5. regulation of positive chemotaxis Source: dictyBase

    Keywords - Molecular functioni

    Kinase, Serine/threonine-protein kinase, Transferase

    Keywords - Ligandi

    ATP-binding, Metal-binding, Nucleotide-binding, Zinc

    Enzyme and pathway databases

    BRENDAi2.7.11.7. 1939.
    SABIO-RKP34125.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Diacylglycerol kinase A (EC:2.7.1.107)
    Alternative name(s):
    Myosin heavy chain kinase
    Short name:
    MHCK
    Gene namesi
    Name:dgkA
    Synonyms:mhcK
    ORF Names:DDB_G0277223
    OrganismiDictyostelium discoideum (Slime mold)
    Taxonomic identifieri44689 [NCBI]
    Taxonomic lineageiEukaryotaAmoebozoaMycetozoaDictyosteliidaDictyostelium
    ProteomesiUP000002195: Chromosome 2, UP000002195: Unassembled WGS sequence

    Organism-specific databases

    dictyBaseiDDB_G0277223. dgkA.

    Subcellular locationi

    Membrane 1 Publication; Peripheral membrane protein 1 Publication

    GO - Cellular componenti

    1. membrane Source: UniProtKB

    Keywords - Cellular componenti

    Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 887887Diacylglycerol kinase APRO_0000218476Add
    BLAST

    Keywords - PTMi

    Phosphoprotein

    Expressioni

    Developmental stagei

    Expressed at low level during vegetative growth and during early development. Higher levels of expression are detected later during development (12-24 hours).2 Publications

    Interactioni

    Protein-protein interaction databases

    STRINGi44689.DDB_0185037.

    Structurei

    3D structure databases

    ProteinModelPortaliP34125.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini330 – 473144DAGKcPROSITE-ProRule annotationAdd
    BLAST

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi72 – 11645Thr-richAdd
    BLAST
    Compositional biasi309 – 3179Poly-Asn
    Compositional biasi720 – 74627Gln-richAdd
    BLAST
    Compositional biasi752 – 80453Poly-AsnAdd
    BLAST
    Compositional biasi827 – 83812Poly-AsnAdd
    BLAST
    Compositional biasi850 – 87122Poly-AsnAdd
    BLAST

    Sequence similaritiesi

    Contains 1 DAGKc domain.PROSITE-ProRule annotation
    Contains 3 phorbol-ester/DAG-type zinc fingers.PROSITE-ProRule annotation

    Zinc finger

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri7 – 5751Phorbol-ester/DAG-type 1PROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri140 – 19051Phorbol-ester/DAG-type 2PROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri208 – 25952Phorbol-ester/DAG-type 3PROSITE-ProRule annotationAdd
    BLAST

    Keywords - Domaini

    Repeat, Zinc-finger

    Phylogenomic databases

    eggNOGiNOG47311.
    KOiK00901.
    OMAiARNANPQ.
    PhylomeDBiP34125.

    Family and domain databases

    InterProiIPR016064. ATP-NAD_kinase_PpnK-typ.
    IPR000756. Diacylglycerol_kin_accessory.
    IPR001206. Diacylglycerol_kinase_cat_dom.
    IPR002219. Prot_Kinase_C-like_PE/DAG-bd.
    [Graphical view]
    PfamiPF00130. C1_1. 3 hits.
    PF00609. DAGK_acc. 1 hit.
    PF00781. DAGK_cat. 1 hit.
    [Graphical view]
    SMARTiSM00109. C1. 3 hits.
    SM00045. DAGKa. 1 hit.
    SM00046. DAGKc. 1 hit.
    [Graphical view]
    SUPFAMiSSF111331. SSF111331. 1 hit.
    PROSITEiPS50146. DAGK. 1 hit.
    PS00479. ZF_DAG_PE_1. 2 hits.
    PS50081. ZF_DAG_PE_2. 3 hits.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P34125-1 [UniParc]FASTAAdd to Basket

    « Hide

    MNQLNISHNW KIKSFTSITF CDHCGSMLWG ICTQGFQCSD CNFSAHSHCT    50
    NLVTLHCNNN KNNSKPNEKP ATITAAAASS STTTTTTTET STNTAASTTT 100
    TTNNIDIKNK DKTPTTSPFS SPILSSYNNN SGNEFCSNRQ HKFHKTSKSI 150
    TKFCNYCRET TFTISGEPVM CSECRYIAHG HCQTKVPLNC IIPYSSPINT 200
    INDGGNVRHH WVEGNLKKSK KCIHCMEPCE KSFSLAHYKC LWCHKYLHSS 250
    CFDKHNPICD FGSLSDMILP PSSIRLLSTT ATTTNKIEKL IIEEQQPQLS 300
    SSPSSPRLNI NNNNNNNEII EWELIENSNM PEKVLFVFVN SKSGGQFGST 350
    LIRKLSSLLN PLQIIDLIKC GGPDSTLQMI NRYLAKHPEQ TNRFRILVCG 400
    GDGTVGWLFK QMTKHLVPST IPIGIIPLGT GNDLARSLGW GIGYDGEKLI 450
    EILKSINEAK TIQMDTWSIE MWDDDKPEDR RVIEMNNYFS IGLDAMVALG 500
    FHLARNANPQ LFTGRTVNKL WYTKIGLEEF VTKNFVSLAR IVKINVGTRE 550
    IRVDRSIEGI IILNLGSYAG GVDLWGANRK DNQTHSDGTG NQFIDDQTLE 600
    IVGVTSLPHL GSCLSSISSP IKMGQADEIR IQVSMPSIIL KDKSNEIETA 650
    FQVDGEPEPI EVRNCTFKIS FFKKVSMLSN RSFKPRIENH DSDLSTFQIS 700
    QSPNLYSPPS FKNPPPNLNQ YQQPHQPPQS QPQPQPQPQQ SLQSPQSPEP 750
    INNNTNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNSNN 800
    NNNNISNDLT NNSEVNPEKS TNNEKTNDNN NNNNNNINTP FEPISLPDEN 850
    NNNNNNNNNN NNNNNNNNNN NETNSEKSYT NNCDEVD 887
    Length:887
    Mass (Da):99,177
    Last modified:December 4, 2007 - v3
    Checksum:iB93209A9876B6132
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti828 – 8281D → E in AAN39880. (PubMed:12296770)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AY152858 Genomic DNA. Translation: AAN39880.1.
    AAFI02000019 Genomic DNA. Translation: EAL68797.1.
    M93393 mRNA. No translation available.
    PIRiA46136.
    RefSeqiXP_642726.1. XM_637634.1.

    Genome annotation databases

    EnsemblProtistsiDDB0185037; DDB0185037; DDB_G0277223.
    GeneIDi8620920.
    KEGGiddi:DDB_G0277223.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AY152858 Genomic DNA. Translation: AAN39880.1 .
    AAFI02000019 Genomic DNA. Translation: EAL68797.1 .
    M93393 mRNA. No translation available.
    PIRi A46136.
    RefSeqi XP_642726.1. XM_637634.1.

    3D structure databases

    ProteinModelPortali P34125.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 44689.DDB_0185037.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblProtistsi DDB0185037 ; DDB0185037 ; DDB_G0277223 .
    GeneIDi 8620920.
    KEGGi ddi:DDB_G0277223.

    Organism-specific databases

    dictyBasei DDB_G0277223. dgkA.

    Phylogenomic databases

    eggNOGi NOG47311.
    KOi K00901.
    OMAi ARNANPQ.
    PhylomeDBi P34125.

    Enzyme and pathway databases

    BRENDAi 2.7.11.7. 1939.
    SABIO-RK P34125.

    Family and domain databases

    InterProi IPR016064. ATP-NAD_kinase_PpnK-typ.
    IPR000756. Diacylglycerol_kin_accessory.
    IPR001206. Diacylglycerol_kinase_cat_dom.
    IPR002219. Prot_Kinase_C-like_PE/DAG-bd.
    [Graphical view ]
    Pfami PF00130. C1_1. 3 hits.
    PF00609. DAGK_acc. 1 hit.
    PF00781. DAGK_cat. 1 hit.
    [Graphical view ]
    SMARTi SM00109. C1. 3 hits.
    SM00045. DAGKa. 1 hit.
    SM00046. DAGKc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF111331. SSF111331. 1 hit.
    PROSITEi PS50146. DAGK. 1 hit.
    PS00479. ZF_DAG_PE_1. 2 hits.
    PS50081. ZF_DAG_PE_2. 3 hits.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Dictyostelium discoideum has a single diacylglycerol kinase gene with similarity to mammalian theta isoforms."
      De La Roche M.A., Smith J.L., Rico M., Carrasco S., Merida I., Licate L., Cote G.P., Egelhoff T.T.
      Biochem. J. 368:809-815(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], CATALYTIC ACTIVITY, DEVELOPMENTAL STAGE.
      Strain: AX2.
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: AX4.
    3. "The genome of the social amoeba Dictyostelium discoideum."
      Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N.
      , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.
      Nature 435:43-57(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: AX4.
    4. "Membrane-bound Dictyostelium myosin heavy chain kinase: a developmentally regulated substrate-specific member of the protein kinase C family."
      Ravid S., Spudich J.A.
      Proc. Natl. Acad. Sci. U.S.A. 89:5877-5881(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: PRELIMINARY PARTIAL NUCLEOTIDE SEQUENCE [MRNA], SUBCELLULAR LOCATION, DEVELOPMENTAL STAGE.
      Strain: AX3.
    5. "Analysis of a novel diacylglycerol kinase from Dictyostelium discoideum: DGKA."
      Ostroski M., Tu-Sekine B., Raben D.M.
      Biochemistry 44:10199-10207(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: CATALYTIC ACTIVITY, BIOPHYSICOCHEMICAL PROPERTIES.
    6. "Analysis of two diacylglycerol kinase activities in mixed micelles."
      Tu-Sekine B., Ostroski M., Raben D.M.
      Adv. Enzyme Regul. 46:12-24(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: CATALYTIC ACTIVITY, BIOPHYSICOCHEMICAL PROPERTIES.

    Entry informationi

    Entry nameiDGKA_DICDI
    AccessioniPrimary (citable) accession number: P34125
    Secondary accession number(s): Q54ZZ0, Q86AQ4, Q8I8F4
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1994
    Last sequence update: December 4, 2007
    Last modified: October 1, 2014
    This is version 110 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)

    Miscellaneousi

    Caution

    The initially described myosin heavy chain kinase activity is probably artifactual due to numerous errors in the sequence used for characterization.1 Publication

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Dictyostelium discoideum
      Dictyostelium discoideum: entries, gene names and cross-references to dictyBase
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3