P34121 (COAA_DICDI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Coactosin

Alternative name(s):
Cyclic AMP-regulated protein p16
Cytoskeletal protein p17

Gene names

Name:	coaA
Synonyms:	p17
ORF Names:	DDB_G0293898

Organism

Dictyostelium discoideum (Slime mold)

Taxonomic identifier

44689 [NCBI]

Taxonomic lineage

Eukaryota › Amoebozoa › Mycetozoa › Dictyosteliida › Dictyostelium

Protein attributes

Sequence length	146 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Binds to F-actin in a calcium independent manner. Binds to the filaments along their length.
Subcellular location	Cytoplasm › cytoskeleton.
Post-translational modification	The N-terminus is blocked.
Sequence similarities	Belongs to the actin-binding proteins ADF family. Coactosin subfamily. Contains 1 ADF-H domain.

Ontologies

Keywords
Cellular component	Cytoplasm Cytoskeleton
Ligand	Actin-binding
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological process	regulation of actin filament polymerization Inferred from direct assay. Source: dictyBase response to bacterium Inferred from expression pattern. Source: dictyBase
Cellular component	actin cytoskeleton Inferred by curator Ref.1. Source: dictyBase centrosome Inferred from direct assay Ref.4. Source: dictyBase phagocytic vesicle Inferred from direct assay Ref.5. Source: dictyBase
Molecular function	actin filament binding Inferred from direct assay Ref.1. Source: dictyBase
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 146	146	Coactosin		PRO_0000214956
Regions
	Domain	1 – 132	132	ADF-H

Sequences

	Sequence		Length	Mass (Da)
	P34121 [UniParc]. Last modified February 1, 1994. Version 1. Checksum: EE7AE2432D8FE1D9	FASTA	146	16,004
	10 20 30 40 50 60 MADVSSTELK AAYDEVLADS NDTNWCLFKY EGKNKIVLSG KGSGGFAELA QEINQPSERL 70 80 90 100 110 120 YAYLRVVSGD DESKRSKFVF ISWCGEEVGP LAKANVSVHK ASVKQVIKNI GVEVHYTVAD 130 140 DLNEEELMTK VRKSSGADYS GNKSTN

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Coactosin, a 17 kDa F-actin binding protein from Dictyostelium discoideum." de Hostos E.L., Bradtke B., Lottspeich F., Gerisch G. Cell Motil. Cytoskeleton 26:181-191(1993) [PubMed: 8293475] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 128-133. Strain: AX2.
[2]	"Cloning and characterization of a Dictyostelium discoideum cDNA coding for p16, a novel cyclic AMP-regulated protein with a nuclear localization signal." Sastry R., Abbruzzi G., Tauber A.I. Submitted (OCT-1993) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: AX3.
[3]	"The genome of the social amoeba Dictyostelium discoideum." Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N. , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A. Nature 435:43-57(2005) [PubMed: 15875012] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: AX4.
[4]	"Identification of novel centrosomal proteins in Dictyostelium discoideum by comparative proteomic approaches." Reinders Y., Schulz I., Graef R., Sickmann A. J. Proteome Res. 5:589-598(2006) [PubMed: 16512674] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[5]	"Proteomics fingerprinting of phagosome maturation and evidence for the role of a Galpha during uptake." Gotthardt D., Blancheteau V., Bosserhoff A., Ruppert T., Delorenzi M., Soldati T. Mol. Cell. Proteomics 5:2228-2243(2006) [PubMed: 16926386] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]. Strain: AX2.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X61797 mRNA. Translation: CAA43899.1. M92992 mRNA. Translation: AAA33174.1. AAFI02000224 Genomic DNA. Translation: EAL60448.1.
PIR	S17111.
RefSeq	XP_628885.1. XM_628883.1.
3D structure databases
ProteinModelPortal	P34121.
ModBase	Search...
Protein-protein interaction databases
IntAct	P34121. 1 interaction.
2D gel databases
SWISS-2DPAGE	P34121.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblProtists	DDB0215369; DDB0215369; DDB_G0293898.
GeneID	8629500.
GenomeReviews	Gene locus coaA in contig CM000155_GR.
KEGG	ddi:DDB_G0293898.
Organism-specific databases
dictyBase	DDB_G0293898. coaA.
Phylogenomic databases
GeneTree	EPrGT00050000003554.
HOGENOM	HBG444725.
OMA	GADYNGG.
PhylomeDB	P34121.
ProtClustDB	CLSZ2497071.
Family and domain databases
InterPro	IPR002108. Actin-bd_cofilin/tropomyosin. [Graphical view]
Pfam	PF00241. Cofilin_ADF. 1 hit. [Graphical view]
SMART	SM00102. ADF. 1 hit. [Graphical view]
PROSITE	PS51263. ADF_H. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

COAA_DICDI

Accession

Primary (citable) accession number: P34121
Secondary accession number(s): Q54B31

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1994
Last sequence update:	February 1, 1994
Last modified:	October 19, 2011
This is version 70 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)