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P34119

- PSA4_DICDI

UniProt

P34119 - PSA4_DICDI

Protein

Proteasome subunit alpha type-4

Gene

psmA4

Organism
Dictyostelium discoideum (Slime mold)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 101 (01 Oct 2014)
      Sequence version 1 (01 Feb 1994)
      Previous versions | rss
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    Functioni

    The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity.

    Catalytic activityi

    Cleavage of peptide bonds with very broad specificity.PROSITE-ProRule annotation

    GO - Molecular functioni

    1. protein binding Source: dictyBase
    2. threonine-type endopeptidase activity Source: UniProtKB-KW

    GO - Biological processi

    1. proteolysis Source: dictyBase
    2. ubiquitin-dependent protein catabolic process Source: InterPro

    Keywords - Molecular functioni

    Hydrolase, Protease, Threonine protease

    Enzyme and pathway databases

    ReactomeiREACT_204617. Antigen processing: Ubiquitination & Proteasome degradation.
    REACT_210566. AUF1 (hnRNP D0) destabilizes mRNA.
    REACT_212010. Regulation of activated PAK-2p34 by proteasome mediated degradation.
    REACT_214682. Cross-presentation of soluble exogenous antigens (endosomes).
    REACT_217211. ER-Phagosome pathway.
    REACT_217636. Autodegradation of the E3 ubiquitin ligase COP1.
    REACT_221036. Separation of Sister Chromatids.
    REACT_225025. APC/C:Cdc20 mediated degradation of Securin.
    REACT_226311. APC/C:Cdh1 mediated degradation of Cdc20 and other APC/C:Cdh1 targeted proteins in late mitosis/early G1.
    REACT_226712. Autodegradation of Cdh1 by Cdh1:APC/C.
    REACT_95813. Ubiquitin Mediated Degradation of Phosphorylated Cdc25A.

    Protein family/group databases

    MEROPSiT01.973.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Proteasome subunit alpha type-4 (EC:3.4.25.1)
    Alternative name(s):
    Proteasome component DD4
    Gene namesi
    Name:psmA4
    Synonyms:prdD
    ORF Names:DDB_G0280969
    OrganismiDictyostelium discoideum (Slime mold)
    Taxonomic identifieri44689 [NCBI]
    Taxonomic lineageiEukaryotaAmoebozoaMycetozoaDictyosteliidaDictyostelium
    ProteomesiUP000002195: Chromosome 3, UP000002195: Unassembled WGS sequence

    Organism-specific databases

    dictyBaseiDDB_G0280969. psmA4.

    Subcellular locationi

    Cytoplasm By similarity. Nucleus By similarity

    GO - Cellular componenti

    1. cytoplasm Source: dictyBase
    2. nucleus Source: dictyBase
    3. proteasome core complex, alpha-subunit complex Source: InterPro

    Keywords - Cellular componenti

    Cytoplasm, Nucleus, Proteasome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 250250Proteasome subunit alpha type-4PRO_0000124108Add
    BLAST

    Proteomic databases

    PRIDEiP34119.

    Interactioni

    Subunit structurei

    The 26S proteasome consists of a 20S proteasome core and two 19S regulatory subunits. The 20S proteasome core is composed of 28 subunits that are arranged in four stacked rings, resulting in a barrel-shaped structure. The two end rings are each formed by seven alpha subunits, and the two central rings are each formed by seven beta subunits. The catalytic chamber with the active sites is on the inside of the barrel By similarity.By similarity

    Protein-protein interaction databases

    STRINGi44689.DDB_0214953.

    Structurei

    3D structure databases

    ProteinModelPortaliP34119.
    SMRiP34119. Positions 2-241.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the peptidase T1A family.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiCOG0638.
    KOiK02728.
    OMAiKQEYKDD.
    PhylomeDBiP34119.

    Family and domain databases

    Gene3Di3.60.20.10. 1 hit.
    InterProiIPR029055. Ntn_hydrolases_N.
    IPR000426. Proteasome_asu_N.
    IPR016050. Proteasome_bsu_CS.
    IPR023332. Proteasome_suA-type.
    IPR001353. Proteasome_sua/b.
    [Graphical view]
    PfamiPF00227. Proteasome. 1 hit.
    PF10584. Proteasome_A_N. 1 hit.
    [Graphical view]
    SMARTiSM00948. Proteasome_A_N. 1 hit.
    [Graphical view]
    SUPFAMiSSF56235. SSF56235. 1 hit.
    PROSITEiPS00388. PROTEASOME_ALPHA_1. 1 hit.
    PS51475. PROTEASOME_ALPHA_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P34119-1 [UniParc]FASTAAdd to Basket

    « Hide

    MARRYDQRTT IFSPEGRVYQ VEYAMTAIRH AGATVGILAK DGIVLAAEKK    50
    TTAKLLDSST SISEKMFKID EHVVCAVAGI TSDANILINY ARLSSQRFFY 100
    QYQEPMPVEQ LVSQICDTKQ GYTQYGGLRP FGVSFLYAGW DRHYGFQLYQ 150
    SDPSGNFAGW KATSIGGENS QVAQSVLRSN YKPDISLKEA LQLALKVLTK 200
    TMDRSNINSE KLEFSYFTKQ GDNVVYHIFT AAELDAFIKE TDLEQETEDN 250
    Length:250
    Mass (Da):28,101
    Last modified:February 1, 1994 - v1
    Checksum:i39387786A34999DC
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L22212 mRNA. Translation: AAA33233.1.
    AAFI02000040 Genomic DNA. Translation: EAL66781.1.
    RefSeqiXP_640858.1. XM_635766.1.

    Genome annotation databases

    EnsemblProtistsiDDB0214953; DDB0214953; DDB_G0280969.
    GeneIDi8622914.
    KEGGiddi:DDB_G0280969.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L22212 mRNA. Translation: AAA33233.1 .
    AAFI02000040 Genomic DNA. Translation: EAL66781.1 .
    RefSeqi XP_640858.1. XM_635766.1.

    3D structure databases

    ProteinModelPortali P34119.
    SMRi P34119. Positions 2-241.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 44689.DDB_0214953.

    Protein family/group databases

    MEROPSi T01.973.

    Proteomic databases

    PRIDEi P34119.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblProtistsi DDB0214953 ; DDB0214953 ; DDB_G0280969 .
    GeneIDi 8622914.
    KEGGi ddi:DDB_G0280969.

    Organism-specific databases

    dictyBasei DDB_G0280969. psmA4.

    Phylogenomic databases

    eggNOGi COG0638.
    KOi K02728.
    OMAi KQEYKDD.
    PhylomeDBi P34119.

    Enzyme and pathway databases

    Reactomei REACT_204617. Antigen processing: Ubiquitination & Proteasome degradation.
    REACT_210566. AUF1 (hnRNP D0) destabilizes mRNA.
    REACT_212010. Regulation of activated PAK-2p34 by proteasome mediated degradation.
    REACT_214682. Cross-presentation of soluble exogenous antigens (endosomes).
    REACT_217211. ER-Phagosome pathway.
    REACT_217636. Autodegradation of the E3 ubiquitin ligase COP1.
    REACT_221036. Separation of Sister Chromatids.
    REACT_225025. APC/C:Cdc20 mediated degradation of Securin.
    REACT_226311. APC/C:Cdh1 mediated degradation of Cdc20 and other APC/C:Cdh1 targeted proteins in late mitosis/early G1.
    REACT_226712. Autodegradation of Cdh1 by Cdh1:APC/C.
    REACT_95813. Ubiquitin Mediated Degradation of Phosphorylated Cdc25A.

    Miscellaneous databases

    PROi P34119.

    Family and domain databases

    Gene3Di 3.60.20.10. 1 hit.
    InterProi IPR029055. Ntn_hydrolases_N.
    IPR000426. Proteasome_asu_N.
    IPR016050. Proteasome_bsu_CS.
    IPR023332. Proteasome_suA-type.
    IPR001353. Proteasome_sua/b.
    [Graphical view ]
    Pfami PF00227. Proteasome. 1 hit.
    PF10584. Proteasome_A_N. 1 hit.
    [Graphical view ]
    SMARTi SM00948. Proteasome_A_N. 1 hit.
    [Graphical view ]
    SUPFAMi SSF56235. SSF56235. 1 hit.
    PROSITEi PS00388. PROTEASOME_ALPHA_1. 1 hit.
    PS51475. PROTEASOME_ALPHA_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Proteasomes from Dictyostelium discoideum: characterization of structure and function."
      Schauer T.M., Nesper M., Kehl M., Lottspeich F., Mueller-Taubenberger A., Gerisch G., Baumeister W.
      J. Struct. Biol. 111:135-147(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: AX2.
    2. "The genome of the social amoeba Dictyostelium discoideum."
      Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N.
      , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.
      Nature 435:43-57(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: AX4.

    Entry informationi

    Entry nameiPSA4_DICDI
    AccessioniPrimary (citable) accession number: P34119
    Secondary accession number(s): Q54UB8
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1994
    Last sequence update: February 1, 1994
    Last modified: October 1, 2014
    This is version 101 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Dictyostelium discoideum
      Dictyostelium discoideum: entries, gene names and cross-references to dictyBase
    2. Peptidase families
      Classification of peptidase families and list of entries
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3