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P34117 (CDK5_DICDI) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 94. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Cyclin-dependent kinase 5 homolog

EC=2.7.11.22
Alternative name(s):
CDC2-like serine/threonine-protein kinase CRP
Cell division protein kinase 5
Gene names
Name:cdk5
Synonyms:crp, crpA
ORF Names:DDB_G0288677
OrganismDictyostelium discoideum (Slime mold) [Reference proteome]
Taxonomic identifier44689 [NCBI]
Taxonomic lineageEukaryotaAmoebozoaMycetozoaDictyosteliidaDictyostelium

Protein attributes

Sequence length292 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Catalytic activity

ATP + a protein = ADP + a phosphoprotein.

Enzyme regulation

Phosphorylation at Thr-14 or Tyr-15 inactivates the enzyme By similarity.

Sequence similarities

Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.

Contains 1 protein kinase domain.

Ontologies

Keywords
   LigandATP-binding
Nucleotide-binding
   Molecular functionKinase
Serine/threonine-protein kinase
Transferase
   PTMPhosphoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processaggregation involved in sorocarp development

Inferred from mutant phenotype PubMed 12074548. Source: dictyBase

cell proliferation

Inferred from mutant phenotype PubMed 12074548. Source: dictyBase

phagocytosis

Inferred from mutant phenotype PubMed 12074548. Source: dictyBase

pinocytosis

Inferred from mutant phenotype PubMed 12074548. Source: dictyBase

protein phosphorylation

Inferred from direct assay PubMed 10102987. Source: dictyBase

regulation of aggregate size involved in sorocarp development

Inferred from mutant phenotype PubMed 12074548. Source: dictyBase

sporulation resulting in formation of a cellular spore

Inferred from mutant phenotype PubMed 12074548. Source: dictyBase

   Cellular_componentcytoplasm

Inferred from direct assay PubMed 21766205. Source: dictyBase

intracellular

Inferred from direct assay PubMed 10102987. Source: dictyBase

nucleoplasm

Inferred from direct assay PubMed 21766205. Source: dictyBase

nucleus

Inferred from direct assay PubMed 21766205. Source: dictyBase

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

calmodulin binding

Inferred from physical interaction PubMed 23063531. Source: dictyBase

cyclin-dependent protein serine/threonine kinase activity

Inferred from direct assay PubMed 10102987. Source: dictyBase

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 292292Cyclin-dependent kinase 5 homolog
PRO_0000085740

Regions

Domain4 – 285282Protein kinase
Nucleotide binding10 – 189ATP By similarity

Sites

Active site1261Proton acceptor By similarity
Binding site331ATP By similarity

Amino acid modifications

Modified residue141Phosphothreonine By similarity
Modified residue151Phosphotyrosine By similarity

Experimental info

Sequence conflict191Y → N in AAA16056. Ref.1
Sequence conflict1891G → L in AAA16056. Ref.1

Sequences

Sequence LengthMass (Da)Tools
P34117 [UniParc].

Last modified December 4, 2007. Version 2.
Checksum: D6829B15C0458526

FASTA29233,219
        10         20         30         40         50         60 
MEKYSKIEKL GEGTYGIVYK AKNRETGEIV ALKRIRLDSE DEGVPCTAIR EISLLKELKH 

        70         80         90        100        110        120 
PNIVRLHDVI HTERKLTLVF EYLDQDLKKY LDECGGEISK PTIKSFMYQL LKGVAFCHDH 

       130        140        150        160        170        180 
RVLHRDLKPQ NLLINRKGEL KLADFGLARA FGIPVRTYSH EVVTLWYRAP DVLMGSRKYS 

       190        200        210        220        230        240 
TPIDIWSAGC IFAEMASGRP LFPGSGTSDQ LFRIFKILGT PNEESWPSIT ELPEYKTDFP 

       250        260        270        280        290 
VHPAHQLSSI VHGLDEKGLN LLSKMLQYDP NQRITAAAAL KHPYFDGLEP IN 

« Hide

References

« Hide 'large scale' references
[1]"The isolation from a unicellular organism, Dictyostelium discoideum, of a highly-related cdc2 gene with characteristics of the PCTAIRE subfamily."
Michaelis C.E., Weeks G.
Biochim. Biophys. Acta 1179:117-124(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]"The genome of the social amoeba Dictyostelium discoideum."
Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N. expand/collapse author list , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.
Nature 435:43-57(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: AX4.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
L00652 mRNA. Translation: AAA16056.1.
AAFI02000120 Genomic DNA. Translation: EAL63070.1.
PIRS40021.

3D structure databases

ProteinModelPortalP34117.
SMRP34117. Positions 1-285.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING44689.DDB_0191155.

Proteomic databases

PRIDEP34117.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblProtistsDDB0191155; DDB0191155; DDB_G0288677.
KEGGddi:DDB_G0288677.

Organism-specific databases

dictyBaseDDB_G0288677. cdk5.

Phylogenomic databases

eggNOGCOG0515.
KOK02090.
OMALDECGGE.
PhylomeDBP34117.

Enzyme and pathway databases

BRENDA2.7.11.22. 1939.

Family and domain databases

InterProIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR002290. Ser/Thr_dual-sp_kinase_dom.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMSSF56112. SSF56112. 1 hit.
PROSITEPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameCDK5_DICDI
AccessionPrimary (citable) accession number: P34117
Secondary accession number(s): Q54II5
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: December 4, 2007
Last modified: April 16, 2014
This is version 94 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)

Relevant documents

SIMILARITY comments

Index of protein domains and families

Dictyostelium discoideum

Dictyostelium discoideum: entries, gene names and cross-references to dictyBase