P34117 (CDK5_DICDI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Cyclin-dependent kinase 5 homolog
EC=2.7.11.22

Alternative name(s):
CDC2-like serine/threonine-protein kinase CRP
Cell division protein kinase 5

Gene names

Name:	cdk5
Synonyms:	crp, crpA
ORF Names:	DDB_G0288677

Organism

Dictyostelium discoideum (Slime mold) [Reference proteome]

Taxonomic identifier

44689 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Amoebozoa › Mycetozoa › Dictyosteliida › Dictyostelium

Protein attributes

Sequence length	292 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Catalytic activity	ATP + a protein = ADP + a phosphoprotein.
Enzyme regulation	Phosphorylation at Thr-14 or Tyr-15 inactivates the enzyme By similarity.
Sequence similarities	Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily. Contains 1 protein kinase domain.

Ontologies

Keywords
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Serine/threonine-protein kinase Transferase
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	aggregation involved in sorocarp development Inferred from mutant phenotype PubMed 12074548. Source: dictyBase cell proliferation Inferred from mutant phenotype PubMed 12074548. Source: dictyBase phagocytosis Inferred from mutant phenotype PubMed 12074548. Source: dictyBase pinocytosis Inferred from mutant phenotype PubMed 12074548. Source: dictyBase regulation of aggregate size involved in sorocarp development Inferred from mutant phenotype PubMed 12074548. Source: dictyBase sporulation resulting in formation of a cellular spore Inferred from mutant phenotype PubMed 12074548. Source: dictyBase
Cellular_component	cytoplasm Inferred from direct assay PubMed 21766205. Source: dictyBase nucleoplasm Inferred from direct assay PubMed 21766205. Source: dictyBase
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW cyclin-dependent protein serine/threonine kinase activity Inferred from direct assay PubMed 10102987. Source: dictyBase
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 292	292	Cyclin-dependent kinase 5 homolog		PRO_0000085740
Regions
	Domain	4 – 285	282	Protein kinase
	Nucleotide binding	10 – 18	9	ATP By similarity
Sites
	Active site	126	1	Proton acceptor By similarity
	Binding site	33	1	ATP By similarity
Amino acid modifications
	Modified residue	14	1	Phosphothreonine By similarity
	Modified residue	15	1	Phosphotyrosine By similarity
Experimental info
	Sequence conflict	19	1	Y → N in AAA16056. Ref.1
	Sequence conflict	189	1	G → L in AAA16056. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P34117 [UniParc]. Last modified December 4, 2007. Version 2. Checksum: D6829B15C0458526	FASTA	292	33,219
	10 20 30 40 50 60 MEKYSKIEKL GEGTYGIVYK AKNRETGEIV ALKRIRLDSE DEGVPCTAIR EISLLKELKH 70 80 90 100 110 120 PNIVRLHDVI HTERKLTLVF EYLDQDLKKY LDECGGEISK PTIKSFMYQL LKGVAFCHDH 130 140 150 160 170 180 RVLHRDLKPQ NLLINRKGEL KLADFGLARA FGIPVRTYSH EVVTLWYRAP DVLMGSRKYS 190 200 210 220 230 240 TPIDIWSAGC IFAEMASGRP LFPGSGTSDQ LFRIFKILGT PNEESWPSIT ELPEYKTDFP 250 260 270 280 290 VHPAHQLSSI VHGLDEKGLN LLSKMLQYDP NQRITAAAAL KHPYFDGLEP IN

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The isolation from a unicellular organism, Dictyostelium discoideum, of a highly-related cdc2 gene with characteristics of the PCTAIRE subfamily." Michaelis C.E., Weeks G. Biochim. Biophys. Acta 1179:117-124(1993) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"The genome of the social amoeba Dictyostelium discoideum." Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N. , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A. Nature 435:43-57(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: AX4.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	L00652 mRNA. Translation: AAA16056.1. AAFI02000120 Genomic DNA. Translation: EAL63070.1.
PIR	S40021.
RefSeq	XP_636601.1. XM_631509.1.
3D structure databases
ProteinModelPortal	P34117.
SMR	P34117. Positions 1-285.
ModBase	Search...
Proteomic databases
PRIDE	P34117.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblProtists	DDB0191155; DDB0191155; DDB_G0288677.
GeneID	8626776.
KEGG	ddi:DDB_G0288677.
Organism-specific databases
dictyBase	DDB_G0288677. cdk5.
Phylogenomic databases
eggNOG	COG0515.
KO	K02090.
OMA	GVAFCHD.
Enzyme and pathway databases
BRENDA	2.7.11.22. 1939.
Family and domain databases
InterPro	IPR011009. Kinase-like_dom. IPR000719. Prot_kinase_cat_dom. IPR017441. Protein_kinase_ATP_BS. IPR002290. Ser/Thr_dual-sp_kinase_dom. IPR008271. Ser/Thr_kinase_AS. [Graphical view]
Pfam	PF00069. Pkinase. 1 hit. [Graphical view]
SMART	SM00220. S_TKc. 1 hit. [Graphical view]
SUPFAM	SSF56112. Kinase_like. 1 hit.
PROSITE	PS00107. PROTEIN_KINASE_ATP. 1 hit. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00108. PROTEIN_KINASE_ST. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CDK5_DICDI

Accession

Primary (citable) accession number: P34117
Secondary accession number(s): Q54II5

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1994
Last sequence update:	December 4, 2007
Last modified:	May 1, 2013
This is version 90 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)