P34114 (PHS2_DICDI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glycogen phosphorylase 2
Short name=GP2
EC=2.4.1.1

Gene names

Name:	glpD
ORF Names:	DDB_G0291123

Organism

Dictyostelium discoideum (Slime mold)

Taxonomic identifier

44689 [NCBI]

Taxonomic lineage

Eukaryota › Amoebozoa › Mycetozoa › Dictyosteliida › Dictyostelium

Protein attributes

Sequence length	993 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Phosphorylase is an important allosteric enzyme in carbohydrate metabolism. Enzymes from different sources differ in their regulatory mechanisms and in their natural substrates. However, all known phosphorylases share catalytic and structural properties.
Catalytic activity	(1,4-alpha-D-glucosyl)(n) + phosphate = (1,4-alpha-D-glucosyl)(n-1) + alpha-D-glucose 1-phosphate.
Cofactor	Pyridoxal phosphate.
Subunit structure	Homodimer. Ref.1
Developmental stage	Appears during cell differentiation; absent in amoebae and early stages of development, reaches a maximum level of expression at the slug stage and then decreases. Ref.1
Post-translational modification	The N-terminus is blocked. Enzyme activity requires processing of the 113 kDa peptide to an enzymatically active 106 kDa form of the protein. Processing would occur near the middle of the Gln-rich repetitive element.
Miscellaneous	In D.discoideum glycogen phosphorylase exists as 2 developmentally regulated forms, GP1 and GP2, which are the products of separate genes.
Sequence similarities	Belongs to the glycogen phosphorylase family.

Ontologies

Keywords
Biological process	Carbohydrate metabolism Glycogen metabolism
Ligand	Pyridoxal phosphate
Molecular function	Glycosyltransferase Transferase
Technical term	Allosteric enzyme Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological process	glycogen catabolic process Inferred from direct assay. Source: dictyBase
Cellular component	intracellular Inferred from direct assay. Source: dictyBase
Molecular function	glycogen phosphorylase activity Inferred from direct assay. Source: dictyBase pyridoxal phosphate binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 993	993	Glycogen phosphorylase 2		PRO_0000188547
Regions
	Compositional bias	44 – 75	32	Gln-rich
Sites
	Site	229	1	Involved in the association of subunits By similarity
	Site	242	1	May be involved in allosteric control By similarity
Amino acid modifications
	Modified residue	763	1	N6-(pyridoxal phosphate)lysine By similarity
Experimental info
	Sequence conflict	5	1	R → T in AAA33211. Ref.1
	Sequence conflict	268	1	Y → L in AAA33211. Ref.1
	Sequence conflict	316	1	V → I in AAA33211. Ref.1
	Sequence conflict	414 – 415	2	HN → T in AAA33211. Ref.1
	Sequence conflict	524	1	R → V in AAA33211. Ref.1
	Sequence conflict	533	1	S → A in AAA33211. Ref.1
	Sequence conflict	558	1	I → V in AAA33211. Ref.1
	Sequence conflict	565	1	N → S in AAA33211. Ref.1
	Sequence conflict	568	1	N → S in AAA33211. Ref.1
	Sequence conflict	573 – 574	2	RR → SS in AAA33211. Ref.1
	Sequence conflict	577	1	Q → E in AAA33211. Ref.1
	Sequence conflict	621 – 623	3	EIK → TIN in AAA33211. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P34114 [UniParc]. Last modified December 4, 2007. Version 2. Checksum: 3DD37DBFEE4F2871	FASTA	993	113,104
	10 20 30 40 50 60 MEEKRSTNSP GFDKPKLNRS GSITSATSHP PRSNSNPKLV AKHQQQLYEE SKNKRNQEQQ 70 80 90 100 110 120 NQQPQQQQQK QTSNQSEDPA TQLSSLKFES DKEKEQALLW AFLASYLPED KGSLQKEFVK 130 140 150 160 170 180 HVEYTLAQTK SECTDFSSFQ ALSYCTRDRL IERWKDTKLF FKQKNVKQVN YMSLEFLLGR 190 200 210 220 230 240 SLQNSLSALG LVGKYSDALM DLGFKLEDLY DEERDAGLGN GGLGRLAACF MDSLATCNFP 250 260 270 280 290 300 GYGYGLRYKF GMFYQTLVDG EQVELPDYWL NYGSPWEIER LDVSYPINFY GKVSEVEDEN 310 320 330 340 350 360 GKKVMKWDQG EQMLAVAYDY PIPGFKTYNT VAIRLWSSKP SDEFNLDSFN RGDYLGAIEE 370 380 390 400 410 420 KEKSENITNV LYPNDNTMQG KELRLKQQYL FVSATIQDII SQFKETGKPF SEFHNFHAIQ 430 440 450 460 470 480 LNDTHPTLGI PELMRILIDE EKKSWDEAWD ITTKTFSYTN HTVLPEALEK WSVSMVENVL 490 500 510 520 530 540 PRHIMIIYEI NERFLKLVDQ KWPGDMSKRR ALSIIDESDG KFIRMAFLAI VGSHTINGVA 550 560 570 580 590 600 YLHSELVKHD VFPLFYEIWP NKFQNKTNGV TPRRWIQQSN PQLAELITRS LNSDRWLVNL 610 620 630 640 650 660 DIIKDLVHLA DNSSFQKEWM EIKRNNKIRL AKYIEKRCDI QVNVDVLFDV QVKRFHEYKR 670 680 690 700 710 720 QLLNVLSVIN RYLDIKEGKK VAPRVVIFGG KAAPGYYMAK LIIKLINSVA DVVNNDPKVG 730 740 750 760 770 780 DLLKVVFIPN YCVSNAEIII PASDISQHIS TAGTEASGTS NMKFSMNGGL IIGTLDGANI 790 800 810 820 830 840 EIRDAIGHEN MYIFGARSEE VNKVKKIIHD GKFTPDTRWA RVLTAIKEDT FGPHEQFQDI 850 860 870 880 890 900 INSVSGGNDH YILSYDFGSY LDIQNSIDQD FKDRAKWAKK SIMASVCCGK FSSDRTIKEY 910 920 930 940 950 960 AQQIWGIEEW KRPGPVPVSN EEARSLLVPP PSGSPNDINA ISIERLSPLT FVKQTSASPL 970 980 990 SVISGGDKTN NTLKPKQTTK GFNIGGQPGN PTN

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning, structural analysis, and expression of the glycogen phosphorylase-2 gene in Dictyostelium." Rutherford C.L., Peery R.B., Sucic J.F., Yin Y., Rogers P.V., Luo S., Selmin O. J. Biol. Chem. 267:2294-2302(1992) [PubMed: 1310312] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE, SUBUNIT, PROTEOLYTIC PROCESSING, DEVELOPMENTAL STAGE. Strain: AX3.
[2]	"The genome of the social amoeba Dictyostelium discoideum." Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N. , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A. Nature 435:43-57(2005) [PubMed: 15875012] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: AX4.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M77492 Genomic DNA. Translation: AAA33211.1. AAFI02000175 Genomic DNA. Translation: EAL61845.1.
PIR	A42318.
RefSeq	XP_635369.1. XM_630277.1.
3D structure databases
ProteinModelPortal	P34114.
ModBase	Search...
Protein-protein interaction databases
STRING	P34114.
Protein family/group databases
CAZy	GT35. Glycosyltransferase Family 35.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblProtists	DDB0191397; DDB0191397; DDB_G0291123.
GeneID	8628017.
GenomeReviews	Gene locus glpD in contig CM000154_GR.
KEGG	ddi:DDB_G0291123.
Organism-specific databases
dictyBase	DDB_G0291123. glpD.
Phylogenomic databases
eggNOG	KOG2099.
GeneTree	EPrGT00050000005141.
HOGENOM	HBG444050.
OMA	ILNIARV.
PhylomeDB	P34114.
Family and domain databases
InterPro	IPR011833. Glycg_phsphrylas. IPR000811. Glyco_trans_35. [Graphical view]
KO	K00688.
PANTHER	PTHR11468. Glyco_trans_35. 1 hit.
Pfam	PF00343. Phosphorylase. 1 hit. [Graphical view]
PIRSF	PIRSF000460. Pprylas_GlgP. 1 hit.
TIGRFAMs	TIGR02093. P_ylase. 1 hit.
PROSITE	PS00102. PHOSPHORYLASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PHS2_DICDI

Accession

Primary (citable) accession number: P34114
Secondary accession number(s): Q54F21

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1994
Last sequence update:	December 4, 2007
Last modified:	November 16, 2011
This is version 78 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)