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P34112

- CDK1_DICDI

UniProt

P34112 - CDK1_DICDI

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Protein

Cyclin-dependent kinase 1

Gene

cdk1

Organism
Dictyostelium discoideum (Slime mold)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli

Functioni

Plays a key role in the control of the eukaryotic cell cycle. It is required in higher cells for entry into S-phase and mitosis. p34 is a component of the kinase complex that phosphorylates the repetitive C-terminus of RNA polymerase II.

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.
ATP + [DNA-directed RNA polymerase] = ADP + [DNA-directed RNA polymerase] phosphate.

Enzyme regulationi

Phosphorylation at Thr-20 or Tyr-21 inactivates the enzyme, while phosphorylation at Thr-162 activates it.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei39 – 391ATPPROSITE-ProRule annotation
Active sitei129 – 1291Proton acceptorPROSITE-ProRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi16 – 249ATPPROSITE-ProRule annotation

GO - Molecular functioni

  1. ATP binding Source: dictyBase
  2. cyclin-dependent protein serine/threonine kinase activity Source: dictyBase
  3. RNA polymerase II carboxy-terminal domain kinase activity Source: UniProtKB-EC

GO - Biological processi

  1. mitotic nuclear division Source: UniProtKB-KW
  2. protein phosphorylation Source: dictyBase
  3. regulation of cell cycle Source: GOC
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Biological processi

Cell cycle, Cell division, Mitosis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi2.7.11.22. 1939.

Names & Taxonomyi

Protein namesi
Recommended name:
Cyclin-dependent kinase 1 (EC:2.7.11.22, EC:2.7.11.23)
Short name:
CDK1
Alternative name(s):
Cell division control protein 2 homolog
Cell division protein kinase 1
p34 protein kinase
Gene namesi
Name:cdk1
Synonyms:cdcB
ORF Names:DDB_G0272813
OrganismiDictyostelium discoideum (Slime mold)
Taxonomic identifieri44689 [NCBI]
Taxonomic lineageiEukaryotaAmoebozoaMycetozoaDictyosteliidaDictyostelium
ProteomesiUP000002195: Chromosome 2, UP000002195: Unassembled WGS sequence

Organism-specific databases

dictyBaseiDDB_G0272813. cdk1.

Subcellular locationi

GO - Cellular componenti

  1. intracellular Source: dictyBase
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 296296Cyclin-dependent kinase 1PRO_0000085739Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei20 – 201PhosphothreonineBy similarity
Modified residuei21 – 211PhosphotyrosineBy similarity
Modified residuei162 – 1621Phosphothreonine; by CAKBy similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PRIDEiP34112.

Interactioni

Subunit structurei

Forms a stable but non-covalent complex with a regulatory subunit and with a cyclin.

Protein-protein interaction databases

STRINGi44689.DDB_0185028.

Structurei

3D structure databases

ProteinModelPortaliP34112.
SMRiP34112. Positions 5-291.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini10 – 288279Protein kinasePROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 protein kinase domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiCOG0515.
InParanoidiP34112.
KOiK02206.
OMAiDCEIDQI.
PhylomeDBiP34112.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR002290. Ser/Thr_dual-sp_kinase.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P34112-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MESDGGLSRY QKLEKLGEGT YGKVYKAKEK ATGRMVALKK IRLEDDGVPS
60 70 80 90 100
TALREISLLK EVPHPNVVSL FDVLHCQNRL YLVFEYLDQD LKKYMDSVPA
110 120 130 140 150
LCPQLIKSYL YQLLKGLAYS HGHRILHRDL KPQNLLIDRQ GALKLADFGL
160 170 180 190 200
ARAVSIPVRV YTHEIVTLWY RAPEVLLGSK SYSVPVDMWS VGCIFGEMLN
210 220 230 240 250
KKPLFSGDCE IDQIFRIFRV LGTPDDSIWP GVTKLPEYVS TFPNWPGQPY
260 270 280 290
NKIFPRCEPL ALDLIAKMLQ YEPSKRISAK EALLHPYFGD LDTSFF
Length:296
Mass (Da):33,773
Last modified:February 1, 1994 - v1
Checksum:i406B53820AA677B6
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M80808 mRNA. Translation: AAA33178.1.
AAFI02000008 Genomic DNA. Translation: EAL71044.1.
PIRiS24386.
RefSeqiXP_644979.1. XM_639887.1.

Genome annotation databases

EnsemblProtistsiDDB0185028; DDB0185028; DDB_G0272813.
GeneIDi8618656.
KEGGiddi:DDB_G0272813.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M80808 mRNA. Translation: AAA33178.1 .
AAFI02000008 Genomic DNA. Translation: EAL71044.1 .
PIRi S24386.
RefSeqi XP_644979.1. XM_639887.1.

3D structure databases

ProteinModelPortali P34112.
SMRi P34112. Positions 5-291.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 44689.DDB_0185028.

Proteomic databases

PRIDEi P34112.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblProtistsi DDB0185028 ; DDB0185028 ; DDB_G0272813 .
GeneIDi 8618656.
KEGGi ddi:DDB_G0272813.

Organism-specific databases

dictyBasei DDB_G0272813. cdk1.

Phylogenomic databases

eggNOGi COG0515.
InParanoidi P34112.
KOi K02206.
OMAi DCEIDQI.
PhylomeDBi P34112.

Enzyme and pathway databases

BRENDAi 2.7.11.22. 1939.

Miscellaneous databases

PROi P34112.

Family and domain databases

InterProi IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR002290. Ser/Thr_dual-sp_kinase.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view ]
Pfami PF00069. Pkinase. 1 hit.
[Graphical view ]
SMARTi SM00220. S_TKc. 1 hit.
[Graphical view ]
SUPFAMi SSF56112. SSF56112. 1 hit.
PROSITEi PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Isolation and characterization of a cdc 2 cDNA from Dictyostelium discoideum."
    Michaelis C.E., Weeks G.
    Biochim. Biophys. Acta 1132:35-42(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: AX4.
  3. "The genome of the social amoeba Dictyostelium discoideum."
    Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N.
    , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.
    Nature 435:43-57(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: AX4.

Entry informationi

Entry nameiCDK1_DICDI
AccessioniPrimary (citable) accession number: P34112
Secondary accession number(s): Q558T7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: February 1, 1994
Last modified: November 26, 2014
This is version 113 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Dictyostelium discoideum
    Dictyostelium discoideum: entries, gene names and cross-references to dictyBase
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3