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P34112 (CDK1_DICDI) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 101. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Cyclin-dependent kinase 1
Short name=CDK1
EC=2.7.11.22
EC=2.7.11.23
Alternative name(s):
Cell division control protein 2 homolog
Cell division protein kinase 1
p34 protein kinase
Gene names
Name:cdk1
Synonyms:cdcB
ORF Names:DDB_G0272813
OrganismDictyostelium discoideum (Slime mold) [Reference proteome]
Taxonomic identifier44689 [NCBI]
Taxonomic lineageEukaryota › Amoebozoa › Mycetozoa › Dictyosteliida › Dictyostelium

Protein attributes

Sequence length296 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Plays a key role in the control of the eukaryotic cell cycle. It is required in higher cells for entry into S-phase and mitosis. p34 is a component of the kinase complex that phosphorylates the repetitive C-terminus of RNA polymerase II.

Catalytic activity

ATP + a protein = ADP + a phosphoprotein.

ATP + [DNA-directed RNA polymerase] = ADP + [DNA-directed RNA polymerase] phosphate.

Enzyme regulation

Phosphorylation at Thr-20 or Tyr-21 inactivates the enzyme, while phosphorylation at Thr-162 activates it By similarity.

Subunit structure

Forms a stable but non-covalent complex with a regulatory subunit and with a cyclin.

Sequence similarities

Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.

Contains 1 protein kinase domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 296296Cyclin-dependent kinase 1
PRO_0000085739

Regions

Domain10 – 288279Protein kinase
Nucleotide binding16 – 249ATP By similarity

Sites

Active site1291Proton acceptor By similarity
Binding site391ATP By similarity

Amino acid modifications

Modified residue201Phosphothreonine By similarity
Modified residue211Phosphotyrosine By similarity
Modified residue1621Phosphothreonine; by CAK By similarity

Sequences

Sequence LengthMass (Da)Tools
P34112 [UniParc].

Last modified February 1, 1994. Version 1.
Checksum: 406B53820AA677B6

FASTA29633,773
        10         20         30         40         50         60 
MESDGGLSRY QKLEKLGEGT YGKVYKAKEK ATGRMVALKK IRLEDDGVPS TALREISLLK 

        70         80         90        100        110        120 
EVPHPNVVSL FDVLHCQNRL YLVFEYLDQD LKKYMDSVPA LCPQLIKSYL YQLLKGLAYS 

       130        140        150        160        170        180 
HGHRILHRDL KPQNLLIDRQ GALKLADFGL ARAVSIPVRV YTHEIVTLWY RAPEVLLGSK 

       190        200        210        220        230        240 
SYSVPVDMWS VGCIFGEMLN KKPLFSGDCE IDQIFRIFRV LGTPDDSIWP GVTKLPEYVS 

       250        260        270        280        290 
TFPNWPGQPY NKIFPRCEPL ALDLIAKMLQ YEPSKRISAK EALLHPYFGD LDTSFF

« Hide

References

« Hide 'large scale' references
[1]"Isolation and characterization of a cdc 2 cDNA from Dictyostelium discoideum."
Michaelis C.E., Weeks G.
Biochim. Biophys. Acta 1132:35-42(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]"Sequence and analysis of chromosome 2 of Dictyostelium discoideum."
Gloeckner G., Eichinger L., Szafranski K., Pachebat J.A., Bankier A.T., Dear P.H., Lehmann R., Baumgart C., Parra G., Abril J.F., Guigo R., Kumpf K., Tunggal B., Cox E.C., Quail M.A., Platzer M., Rosenthal A., Noegel A.A.
Nature 418:79-85(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: AX4.
[3]"The genome of the social amoeba Dictyostelium discoideum."
Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N. expand/collapse author list , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.
Nature 435:43-57(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: AX4.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		M80808 mRNA. Translation: AAA33178.1.
AAFI02000008 Genomic DNA. Translation: EAL71044.1.
PIRS24386.
RefSeqXP_644979.1. XM_639887.1.

3D structure databases

ProteinModelPortalP34112.
SMRP34112. Positions 5-291.
ModBaseSearch...

Protein-protein interaction databases

STRING44689.DDB_0185028.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblProtistsDDB0185028; DDB0185028; DDB_G0272813.
GeneID8618656.
KEGGddi:DDB_G0272813.

Organism-specific databases

dictyBaseDDB_G0272813. cdk1.

Phylogenomic databases

eggNOGCOG0515.
KOK02206.
OMAPRCEPLA.

Enzyme and pathway databases

BRENDA2.7.11.22. 1939.

Family and domain databases

InterProIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_cat_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR002290. Ser/Thr_dual-sp_kinase_dom.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMSSF56112. Kinase_like. 1 hit.
PROSITEPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameCDK1_DICDI
AccessionPrimary (citable) accession number: P34112
Secondary accession number(s): Q558T7
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: February 1, 1994
Last modified: May 1, 2013
This is version 101 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)

Relevant documents

Dictyostelium discoideum

Dictyostelium discoideum: entries, gene names and cross-references to dictyBase

SIMILARITY comments

Index of protein domains and families

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