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P34112

- CDK1_DICDI

UniProt

P34112 - CDK1_DICDI

Protein

Cyclin-dependent kinase 1

Gene

cdk1

Organism
Dictyostelium discoideum (Slime mold)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 111 (01 Oct 2014)
      Sequence version 1 (01 Feb 1994)
      Previous versions | rss
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    Functioni

    Plays a key role in the control of the eukaryotic cell cycle. It is required in higher cells for entry into S-phase and mitosis. p34 is a component of the kinase complex that phosphorylates the repetitive C-terminus of RNA polymerase II.

    Catalytic activityi

    ATP + a protein = ADP + a phosphoprotein.
    ATP + [DNA-directed RNA polymerase] = ADP + [DNA-directed RNA polymerase] phosphate.

    Enzyme regulationi

    Phosphorylation at Thr-20 or Tyr-21 inactivates the enzyme, while phosphorylation at Thr-162 activates it.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei39 – 391ATPPROSITE-ProRule annotation
    Active sitei129 – 1291Proton acceptorPROSITE-ProRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi16 – 249ATPPROSITE-ProRule annotation

    GO - Molecular functioni

    1. ATP binding Source: dictyBase
    2. cyclin-dependent protein serine/threonine kinase activity Source: dictyBase
    3. protein binding Source: dictyBase
    4. RNA polymerase II carboxy-terminal domain kinase activity Source: UniProtKB-EC

    GO - Biological processi

    1. mitotic nuclear division Source: UniProtKB-KW
    2. protein phosphorylation Source: dictyBase
    3. regulation of cell cycle Source: GOC

    Keywords - Molecular functioni

    Kinase, Serine/threonine-protein kinase, Transferase

    Keywords - Biological processi

    Cell cycle, Cell division, Mitosis

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    BRENDAi2.7.11.22. 1939.
    ReactomeiREACT_202026. Chk1/Chk2(Cds1) mediated inactivation of Cyclin B:Cdk1 complex.
    REACT_204029. Factors involved in megakaryocyte development and platelet production.
    REACT_204065. APC/C:Cdc20 mediated degradation of Cyclin B.
    REACT_208705. G0 and Early G1.
    REACT_209442. ERK1 activation.
    REACT_209924. Regulation of APC/C activators between G1/S and early anaphase.
    REACT_212866. E2F mediated regulation of DNA replication.
    REACT_212891. G1/S-Specific Transcription.
    REACT_213265. Cyclin A/B1 associated events during G2/M transition.
    REACT_214047. E2F-enabled inhibition of pre-replication complex formation.
    REACT_217248. Resolution of Sister Chromatid Cohesion.
    REACT_217719. G2/M DNA replication checkpoint.
    REACT_217775. Cyclin B2 mediated events.
    REACT_217841. MASTL Facilitates Mitotic Progression.
    REACT_219198. Condensation of Prometaphase Chromosomes.
    REACT_220869. Golgi Cisternae Pericentriolar Stack Reorganization.
    REACT_222528. Depolymerisation of the Nuclear Lamina.
    REACT_223655. Phosphorylation of the APC/C.
    REACT_224585. Senescence-Associated Secretory Phenotype (SASP).
    REACT_225362. Condensation of Prophase Chromosomes.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Cyclin-dependent kinase 1 (EC:2.7.11.22, EC:2.7.11.23)
    Short name:
    CDK1
    Alternative name(s):
    Cell division control protein 2 homolog
    Cell division protein kinase 1
    p34 protein kinase
    Gene namesi
    Name:cdk1
    Synonyms:cdcB
    ORF Names:DDB_G0272813
    OrganismiDictyostelium discoideum (Slime mold)
    Taxonomic identifieri44689 [NCBI]
    Taxonomic lineageiEukaryotaAmoebozoaMycetozoaDictyosteliidaDictyostelium
    ProteomesiUP000002195: Chromosome 2, UP000002195: Unassembled WGS sequence

    Organism-specific databases

    dictyBaseiDDB_G0272813. cdk1.

    Subcellular locationi

    GO - Cellular componenti

    1. intracellular Source: dictyBase

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 296296Cyclin-dependent kinase 1PRO_0000085739Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei20 – 201PhosphothreonineBy similarity
    Modified residuei21 – 211PhosphotyrosineBy similarity
    Modified residuei162 – 1621Phosphothreonine; by CAKBy similarity

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    PRIDEiP34112.

    Interactioni

    Subunit structurei

    Forms a stable but non-covalent complex with a regulatory subunit and with a cyclin.

    Protein-protein interaction databases

    STRINGi44689.DDB_0185028.

    Structurei

    3D structure databases

    ProteinModelPortaliP34112.
    SMRiP34112. Positions 5-291.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini10 – 288279Protein kinasePROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Contains 1 protein kinase domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiCOG0515.
    KOiK02206.
    OMAiDCEIDQI.
    PhylomeDBiP34112.

    Family and domain databases

    InterProiIPR011009. Kinase-like_dom.
    IPR000719. Prot_kinase_dom.
    IPR017441. Protein_kinase_ATP_BS.
    IPR002290. Ser/Thr_dual-sp_kinase_dom.
    IPR008271. Ser/Thr_kinase_AS.
    [Graphical view]
    PfamiPF00069. Pkinase. 1 hit.
    [Graphical view]
    SMARTiSM00220. S_TKc. 1 hit.
    [Graphical view]
    SUPFAMiSSF56112. SSF56112. 1 hit.
    PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
    PS50011. PROTEIN_KINASE_DOM. 1 hit.
    PS00108. PROTEIN_KINASE_ST. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P34112-1 [UniParc]FASTAAdd to Basket

    « Hide

    MESDGGLSRY QKLEKLGEGT YGKVYKAKEK ATGRMVALKK IRLEDDGVPS    50
    TALREISLLK EVPHPNVVSL FDVLHCQNRL YLVFEYLDQD LKKYMDSVPA 100
    LCPQLIKSYL YQLLKGLAYS HGHRILHRDL KPQNLLIDRQ GALKLADFGL 150
    ARAVSIPVRV YTHEIVTLWY RAPEVLLGSK SYSVPVDMWS VGCIFGEMLN 200
    KKPLFSGDCE IDQIFRIFRV LGTPDDSIWP GVTKLPEYVS TFPNWPGQPY 250
    NKIFPRCEPL ALDLIAKMLQ YEPSKRISAK EALLHPYFGD LDTSFF 296
    Length:296
    Mass (Da):33,773
    Last modified:February 1, 1994 - v1
    Checksum:i406B53820AA677B6
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M80808 mRNA. Translation: AAA33178.1.
    AAFI02000008 Genomic DNA. Translation: EAL71044.1.
    PIRiS24386.
    RefSeqiXP_644979.1. XM_639887.1.

    Genome annotation databases

    EnsemblProtistsiDDB0185028; DDB0185028; DDB_G0272813.
    GeneIDi8618656.
    KEGGiddi:DDB_G0272813.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M80808 mRNA. Translation: AAA33178.1 .
    AAFI02000008 Genomic DNA. Translation: EAL71044.1 .
    PIRi S24386.
    RefSeqi XP_644979.1. XM_639887.1.

    3D structure databases

    ProteinModelPortali P34112.
    SMRi P34112. Positions 5-291.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 44689.DDB_0185028.

    Proteomic databases

    PRIDEi P34112.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblProtistsi DDB0185028 ; DDB0185028 ; DDB_G0272813 .
    GeneIDi 8618656.
    KEGGi ddi:DDB_G0272813.

    Organism-specific databases

    dictyBasei DDB_G0272813. cdk1.

    Phylogenomic databases

    eggNOGi COG0515.
    KOi K02206.
    OMAi DCEIDQI.
    PhylomeDBi P34112.

    Enzyme and pathway databases

    BRENDAi 2.7.11.22. 1939.
    Reactomei REACT_202026. Chk1/Chk2(Cds1) mediated inactivation of Cyclin B:Cdk1 complex.
    REACT_204029. Factors involved in megakaryocyte development and platelet production.
    REACT_204065. APC/C:Cdc20 mediated degradation of Cyclin B.
    REACT_208705. G0 and Early G1.
    REACT_209442. ERK1 activation.
    REACT_209924. Regulation of APC/C activators between G1/S and early anaphase.
    REACT_212866. E2F mediated regulation of DNA replication.
    REACT_212891. G1/S-Specific Transcription.
    REACT_213265. Cyclin A/B1 associated events during G2/M transition.
    REACT_214047. E2F-enabled inhibition of pre-replication complex formation.
    REACT_217248. Resolution of Sister Chromatid Cohesion.
    REACT_217719. G2/M DNA replication checkpoint.
    REACT_217775. Cyclin B2 mediated events.
    REACT_217841. MASTL Facilitates Mitotic Progression.
    REACT_219198. Condensation of Prometaphase Chromosomes.
    REACT_220869. Golgi Cisternae Pericentriolar Stack Reorganization.
    REACT_222528. Depolymerisation of the Nuclear Lamina.
    REACT_223655. Phosphorylation of the APC/C.
    REACT_224585. Senescence-Associated Secretory Phenotype (SASP).
    REACT_225362. Condensation of Prophase Chromosomes.

    Miscellaneous databases

    PROi P34112.

    Family and domain databases

    InterProi IPR011009. Kinase-like_dom.
    IPR000719. Prot_kinase_dom.
    IPR017441. Protein_kinase_ATP_BS.
    IPR002290. Ser/Thr_dual-sp_kinase_dom.
    IPR008271. Ser/Thr_kinase_AS.
    [Graphical view ]
    Pfami PF00069. Pkinase. 1 hit.
    [Graphical view ]
    SMARTi SM00220. S_TKc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF56112. SSF56112. 1 hit.
    PROSITEi PS00107. PROTEIN_KINASE_ATP. 1 hit.
    PS50011. PROTEIN_KINASE_DOM. 1 hit.
    PS00108. PROTEIN_KINASE_ST. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Isolation and characterization of a cdc 2 cDNA from Dictyostelium discoideum."
      Michaelis C.E., Weeks G.
      Biochim. Biophys. Acta 1132:35-42(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: AX4.
    3. "The genome of the social amoeba Dictyostelium discoideum."
      Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N.
      , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.
      Nature 435:43-57(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: AX4.

    Entry informationi

    Entry nameiCDK1_DICDI
    AccessioniPrimary (citable) accession number: P34112
    Secondary accession number(s): Q558T7
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1994
    Last sequence update: February 1, 1994
    Last modified: October 1, 2014
    This is version 111 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Dictyostelium discoideum
      Dictyostelium discoideum: entries, gene names and cross-references to dictyBase
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3