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Protein

Transcription factor tau 138 kDa subunit

Gene

TFC3

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

TFIIIC mediates tRNA and 5S RNA gene activation by binding to intragenic promoter elements. Upstream of the transcription start site, TFIIIC assembles the initiation complex TFIIIB-TFIIIC-tDNA, which is sufficient for RNA polymerase III recruitment and function. Part of the tauB domain of TFIIIC that binds boxB DNA promoter sites of tRNA and similar genes. TFC3 is essential for cell viability. Cooperates with TFC6 in DNA binding.3 Publications

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-28816-MONOMER.
ReactomeiR-SCE-73780. RNA Polymerase III Chain Elongation.
R-SCE-749476. RNA Polymerase III Abortive And Retractive Initiation.
R-SCE-76061. RNA Polymerase III Transcription Initiation From Type 1 Promoter.
R-SCE-76066. RNA Polymerase III Transcription Initiation From Type 2 Promoter.
R-SCE-76071. RNA Polymerase III Transcription Initiation From Type 3 Promoter.

Names & Taxonomyi

Protein namesi
Recommended name:
Transcription factor tau 138 kDa subunit
Alternative name(s):
TFIIIC 138 kDa subunit
Transcription factor C subunit 3
Gene namesi
Name:TFC3
Synonyms:TSV115
Ordered Locus Names:YAL001C
ORF Names:FUN24
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome I

Organism-specific databases

EuPathDBiFungiDB:YAL001C.
SGDiS000000001. TFC3.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi349 – 3491G → E in TSV115; thermosensitive. Level of TFIIIC and its affinity for tDNA reduced. tDNA binding activity very sensitive to mild heat treatments, and TFIIIC-DNA interaction inhibited at moderate salt concentrations. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 11601160Transcription factor tau 138 kDa subunitPRO_0000072497Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei546 – 5461PhosphoserineCombined sources

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP34111.

PTM databases

iPTMnetiP34111.

Interactioni

Subunit structurei

Component of the TFIIIC complex composed of TFC1, TFC3, TFC4, TFC6, TFC7 and TFC8. The subunits are organized in two globular domains, tauA and tauB, connected by a proteolysis-sensitive and flexible linker. Interacts with TFC1, TFC4 and TFC6.4 Publications

Protein-protein interaction databases

BioGridi31789. 39 interactions.
DIPiDIP-6739N.
IntActiP34111. 3 interactions.
MINTiMINT-636763.

Structurei

Secondary structure

1
1160
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi546 – 56520Combined sources
Beta strandi568 – 5736Combined sources
Helixi574 – 58411Combined sources
Helixi593 – 60412Combined sources
Beta strandi607 – 6126Combined sources
Turni614 – 6163Combined sources
Beta strandi619 – 6224Combined sources
Helixi628 – 63710Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
5AIMX-ray1.40A/B546-641[»]
ProteinModelPortaliP34111.
SMRiP34111. Positions 546-640.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Phylogenomic databases

HOGENOMiHOG000142056.
InParanoidiP34111.
KOiK15204.
OMAiMSSMIQR.
OrthoDBiEOG092C06YM.

Family and domain databases

InterProiIPR007309. TFIIIC_Bblock-bd.
[Graphical view]
PfamiPF04182. B-block_TFIIIC. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P34111-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVLTIYPDEL VQIVSDKIAS NKGKITLNQL WDISGKYFDL SDKKVKQFVL
60 70 80 90 100
SCVILKKDIE VYCDGAITTK NVTDIIGDAN HSYSVGITED SLWTLLTGYT
110 120 130 140 150
KKESTIGNSA FELLLEVAKS GEKGINTMDL AQVTGQDPRS VTGRIKKINH
160 170 180 190 200
LLTSSQLIYK GHVVKQLKLK KFSHDGVDSN PYINIRDHLA TIVEVVKRSK
210 220 230 240 250
NGIRQIIDLK RELKFDKEKR LSKAFIAAIA WLDEKEYLKK VLVVSPKNPA
260 270 280 290 300
IKIRCVKYVK DIPDSKGSPS FEYDSNSADE DSVSDSKAAF EDEDLVEGLD
310 320 330 340 350
NFNATDLLQN QGLVMEEKED AVKNEVLLNR FYPLQNQTYD IADKSGLKGI
360 370 380 390 400
STMDVVNRIT GKEFQRAFTK SSEYYLESVD KQKENTGGYR LFRIYDFEGK
410 420 430 440 450
KKFFRLFTAQ NFQKLTNAED EISVPKGFDE LGKSRTDLKT LNEDNFVALN
460 470 480 490 500
NTVRFTTDSD GQDIFFWHGE LKIPPNSKKT PNKNKRKRQV KNSTNASVAG
510 520 530 540 550
NISNPKRIKL EQHVSTAQEP KSAEDSPSSN GGTVVKGKVV NFGGFSARSL
560 570 580 590 600
RSLQRQRAIL KVMNTIGGVA YLREQFYESV SKYMGSTTTL DKKTVRGDVD
610 620 630 640 650
LMVESEKLGA RTEPVSGRKI IFLPTVGEDA IQRYILKEKD SKKATFTDVI
660 670 680 690 700
HDTEIYFFDQ TEKNRFHRGK KSVERIRKFQ NRQKNAKIKA SDDAISKKST
710 720 730 740 750
SVNVSDGKIK RRDKKVSAGR TTVVVENTKE DKTVYHAGTK DGVQALIRAV
760 770 780 790 800
VVTKSIKNEI MWDKITKLFP NNSLDNLKKK WTARRVRMGH SGWRAYVDKW
810 820 830 840 850
KKMLVLAIKS EKISLRDVEE LDLIKLLDIW TSFDEKEIKR PLFLYKNYEE
860 870 880 890 900
NRKKFTLVRD DTLTHSGNDL AMSSMIQREI SSLKKTYTRK ISASTKDLSK
910 920 930 940 950
SQSDDYIRTV IRSILIESPS TTRNEIEALK NVGNESIDNV IMDMAKEKQI
960 970 980 990 1000
YLHGSKLECT DTLPDILENR GNYKDFGVAF QYRCKVNELL EAGNAIVINQ
1010 1020 1030 1040 1050
EPSDISSWVL IDLISGELLN MDVIPMVRNV RPLTYTSRRF EIRTLTPPLI
1060 1070 1080 1090 1100
IYANSQTKLN TARKSAVKVP LGKPFSRLWV NGSGSIRPNI WKQVVTMVVN
1110 1120 1130 1140 1150
EIIFHPGITL SRLQSRCREV LSLHEISEIC KWLLERQVLI TTDFDGYWVN
1160
HNWYSIYEST
Length:1,160
Mass (Da):132,108
Last modified:February 1, 1994 - v1
Checksum:i3ACB7893ED536581
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M98261 Genomic DNA. Translation: AAA34378.1.
L22015 Genomic DNA. Translation: AAC04956.1.
BK006935 Genomic DNA. Translation: DAA06985.1.
PIRiA46423.
RefSeqiNP_009400.1. NM_001178148.1.

Genome annotation databases

EnsemblFungiiYAL001C; YAL001C; YAL001C.
GeneIDi851262.
KEGGisce:YAL001C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M98261 Genomic DNA. Translation: AAA34378.1.
L22015 Genomic DNA. Translation: AAC04956.1.
BK006935 Genomic DNA. Translation: DAA06985.1.
PIRiA46423.
RefSeqiNP_009400.1. NM_001178148.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
5AIMX-ray1.40A/B546-641[»]
ProteinModelPortaliP34111.
SMRiP34111. Positions 546-640.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi31789. 39 interactions.
DIPiDIP-6739N.
IntActiP34111. 3 interactions.
MINTiMINT-636763.

PTM databases

iPTMnetiP34111.

Proteomic databases

MaxQBiP34111.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYAL001C; YAL001C; YAL001C.
GeneIDi851262.
KEGGisce:YAL001C.

Organism-specific databases

EuPathDBiFungiDB:YAL001C.
SGDiS000000001. TFC3.

Phylogenomic databases

HOGENOMiHOG000142056.
InParanoidiP34111.
KOiK15204.
OMAiMSSMIQR.
OrthoDBiEOG092C06YM.

Enzyme and pathway databases

BioCyciYEAST:G3O-28816-MONOMER.
ReactomeiR-SCE-73780. RNA Polymerase III Chain Elongation.
R-SCE-749476. RNA Polymerase III Abortive And Retractive Initiation.
R-SCE-76061. RNA Polymerase III Transcription Initiation From Type 1 Promoter.
R-SCE-76066. RNA Polymerase III Transcription Initiation From Type 2 Promoter.
R-SCE-76071. RNA Polymerase III Transcription Initiation From Type 3 Promoter.

Miscellaneous databases

PROiP34111.

Family and domain databases

InterProiIPR007309. TFIIIC_Bblock-bd.
[Graphical view]
PfamiPF04182. B-block_TFIIIC. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiTFC3_YEAST
AccessioniPrimary (citable) accession number: P34111
Secondary accession number(s): D6VPL5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: February 1, 1994
Last modified: September 7, 2016
This is version 133 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 125 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome I
    Yeast (Saccharomyces cerevisiae) chromosome I: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.