P34111 (TFC3_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Transcription factor tau 138 kDa subunit

Alternative name(s):
TFIIIC 138 kDa subunit
Transcription factor C subunit 3

Gene names

Name:	TFC3
Synonyms:	TSV115
Ordered Locus Names:	YAL001C
ORF Names:	FUN24

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	1160 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	TFIIIC mediates tRNA and 5S RNA gene activation by binding to intragenic promoter elements. Upstream of the transcription start site, TFIIIC assembles the initiation complex TFIIIB-TFIIIC-tDNA, which is sufficient for RNA polymerase III recruitment and function. Part of the tauB domain of TFIIIC that binds boxB DNA promoter sites of tRNA and similar genes. TFC3 is essential for cell viability. Cooperates with TFC6 in DNA binding. Ref.1 Ref.6 Ref.9
Subunit structure	Component of the TFIIIC complex composed of TFC1, TFC3, TFC4, TFC6, TFC7 and TFC8. The subunits are organized in two globular domains, tauA and tauB, connected by a proteolysis-sensitive and flexible linker. Interacts with TFC1, TFC4 and TFC6. Ref.8 Ref.10
Subcellular location	Nucleus. Mitochondrion Ref.11 Ref.13 Ref.14.
Miscellaneous	Present with 125 molecules/cell in log phase SD medium. Ref.12

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Mitochondrion Nucleus
Ligand	DNA-binding
PTM	Phosphoprotein
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological process	protein localization to chromatin Inferred from mutant phenotype. Source: SGD regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW transcription initiation from RNA polymerase III promoter Traceable author statement. Source: SGD
Cellular component	mitochondrion Inferred from direct assay Ref.13Ref.14. Source: SGD transcription factor TFIIIC complex Inferred from direct assay Ref.1Ref.7. Source: SGD
Molecular function	DNA binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1160	1160	Transcription factor tau 138 kDa subunit		PRO_0000072497
Amino acid modifications
	Modified residue	546	1	Phosphoserine Ref.15
Experimental info
	Mutagenesis	349	1	G → E in TSV115; thermosensitive. Level of TFIIIC and its affinity for tDNA reduced. tDNA binding activity very sensitive to mild heat treatments, and TFIIIC-DNA interaction inhibited at moderate salt concentrations. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	P34111 [UniParc]. Last modified February 1, 1994. Version 1. Checksum: 3ACB7893ED536581	FASTA	1,160	132,108
	10 20 30 40 50 60 MVLTIYPDEL VQIVSDKIAS NKGKITLNQL WDISGKYFDL SDKKVKQFVL SCVILKKDIE 70 80 90 100 110 120 VYCDGAITTK NVTDIIGDAN HSYSVGITED SLWTLLTGYT KKESTIGNSA FELLLEVAKS 130 140 150 160 170 180 GEKGINTMDL AQVTGQDPRS VTGRIKKINH LLTSSQLIYK GHVVKQLKLK KFSHDGVDSN 190 200 210 220 230 240 PYINIRDHLA TIVEVVKRSK NGIRQIIDLK RELKFDKEKR LSKAFIAAIA WLDEKEYLKK 250 260 270 280 290 300 VLVVSPKNPA IKIRCVKYVK DIPDSKGSPS FEYDSNSADE DSVSDSKAAF EDEDLVEGLD 310 320 330 340 350 360 NFNATDLLQN QGLVMEEKED AVKNEVLLNR FYPLQNQTYD IADKSGLKGI STMDVVNRIT 370 380 390 400 410 420 GKEFQRAFTK SSEYYLESVD KQKENTGGYR LFRIYDFEGK KKFFRLFTAQ NFQKLTNAED 430 440 450 460 470 480 EISVPKGFDE LGKSRTDLKT LNEDNFVALN NTVRFTTDSD GQDIFFWHGE LKIPPNSKKT 490 500 510 520 530 540 PNKNKRKRQV KNSTNASVAG NISNPKRIKL EQHVSTAQEP KSAEDSPSSN GGTVVKGKVV 550 560 570 580 590 600 NFGGFSARSL RSLQRQRAIL KVMNTIGGVA YLREQFYESV SKYMGSTTTL DKKTVRGDVD 610 620 630 640 650 660 LMVESEKLGA RTEPVSGRKI IFLPTVGEDA IQRYILKEKD SKKATFTDVI HDTEIYFFDQ 670 680 690 700 710 720 TEKNRFHRGK KSVERIRKFQ NRQKNAKIKA SDDAISKKST SVNVSDGKIK RRDKKVSAGR 730 740 750 760 770 780 TTVVVENTKE DKTVYHAGTK DGVQALIRAV VVTKSIKNEI MWDKITKLFP NNSLDNLKKK 790 800 810 820 830 840 WTARRVRMGH SGWRAYVDKW KKMLVLAIKS EKISLRDVEE LDLIKLLDIW TSFDEKEIKR 850 860 870 880 890 900 PLFLYKNYEE NRKKFTLVRD DTLTHSGNDL AMSSMIQREI SSLKKTYTRK ISASTKDLSK 910 920 930 940 950 960 SQSDDYIRTV IRSILIESPS TTRNEIEALK NVGNESIDNV IMDMAKEKQI YLHGSKLECT 970 980 990 1000 1010 1020 DTLPDILENR GNYKDFGVAF QYRCKVNELL EAGNAIVINQ EPSDISSWVL IDLISGELLN 1030 1040 1050 1060 1070 1080 MDVIPMVRNV RPLTYTSRRF EIRTLTPPLI IYANSQTKLN TARKSAVKVP LGKPFSRLWV 1090 1100 1110 1120 1130 1140 NGSGSIRPNI WKQVVTMVVN EIIFHPGITL SRLQSRCREV LSLHEISEIC KWLLERQVLI 1150 1160 TTDFDGYWVN HNWYSIYEST

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"TFC3: gene encoding the B-block binding subunit of the yeast transcription factor IIIC." Lefebvre O., Carles C., Conesa C., Swanson R.N., Bouet F., Riva M., Sentenac A. Proc. Natl. Acad. Sci. U.S.A. 89:10512-10516(1992) [PubMed: 1279682] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 124-143; 331-347; 440-453; 508-520 AND 539-547, FUNCTION, IDENTIFICATION IN TFIIIC. Strain: ATCC 204508 / S288c.
[2]	"A mutation in the largest subunit of yeast TFIIIC affects tRNA and 5 S RNA synthesis. Identification of two classes of suppressors." Lefebvre O., Rueth J., Sentenac A. J. Biol. Chem. 269:23374-23381(1994) [PubMed: 8083243] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], MUTAGENESIS OF GLY-349.
[3]	"Sequencing of chromosome I of Saccharomyces cerevisiae: analysis of the 42 kbp SPO7-CENI-CDC15 region." Clark M.W., Keng T., Storms R.K., Zhong W.-W., Fortin N., Zeng B., Delaney S., Ouellette B.F.F., Barton A.B., Kaback D.B., Bussey H. Yeast 10:535-541(1994) [PubMed: 7941740] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[4]	"The nucleotide sequence of chromosome I from Saccharomyces cerevisiae." Bussey H., Kaback D.B., Zhong W.-W., Vo D.H., Clark M.W., Fortin N., Hall J., Ouellette B.F.F., Keng T., Barton A.B., Su Y., Davies C.J., Storms R.K. Proc. Natl. Acad. Sci. U.S.A. 92:3809-3813(1995) [PubMed: 7731988] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[5]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[6]	"Two polypeptide chains in yeast transcription factor tau interact with DNA." Gabrielsen O.S., Marzouki N., Ruet A., Sentenac A., Fromageot P. J. Biol. Chem. 264:7505-7511(1989) [PubMed: 2651441] [Abstract] Cited for: FUNCTION.
[7]	"Purification and characterization of Saccharomyces cerevisiae transcription factor TFIIIC. Polypeptide composition defined with polyclonal antibodies." Parsons M.C., Weil P.A. J. Biol. Chem. 265:5095-5103(1990) [PubMed: 2180956] [Abstract] Cited for: IDENTIFICATION IN TFIIIC.
[8]	"On the subunit composition, stoichiometry, and phosphorylation of the yeast transcription factor TFIIIC/tau." Conesa C., Swanson R.N., Schultz P., Oudet P., Sentenac A. J. Biol. Chem. 268:18047-18052(1993) [PubMed: 7688737] [Abstract] Cited for: INTERACTION WITH TFC1; TFC4 AND TFC6, PHOSPHORYLATION.
[9]	"Tau91, an essential subunit of yeast transcription factor IIIC, cooperates with tau138 in DNA binding." Arrebola R., Manaud N., Rozenfeld S., Marsolier M.C., Lefebvre O., Carles C., Thuriaux P., Conesa C., Sentenac A. Mol. Cell. Biol. 18:1-9(1998) [PubMed: 9418847] [Abstract] Cited for: FUNCTION.
[10]	"The tau95 subunit of yeast TFIIIC influences upstream and downstream functions of TFIIIC.DNA complexes." Jourdain S., Acker J., Ducrot C., Sentenac A., Lefebvre O. J. Biol. Chem. 278:10450-10457(2003) [PubMed: 12533520] [Abstract] Cited for: INTERACTION WITH TFC1 AND TFC6.
[11]	"Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed: 14562095] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[12]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[13]	"The proteome of Saccharomyces cerevisiae mitochondria." Sickmann A., Reinders J., Wagner Y., Joppich C., Zahedi R.P., Meyer H.E., Schoenfisch B., Perschil I., Chacinska A., Guiard B., Rehling P., Pfanner N., Meisinger C. Proc. Natl. Acad. Sci. U.S.A. 100:13207-13212(2003) [PubMed: 14576278] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS], MASS SPECTROMETRY. Strain: ATCC 76625 / YPH499.
[14]	"Toward the complete yeast mitochondrial proteome: multidimensional separation techniques for mitochondrial proteomics." Reinders J., Zahedi R.P., Pfanner N., Meisinger C., Sickmann A. J. Proteome Res. 5:1543-1554(2006) [PubMed: 16823961] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS], MASS SPECTROMETRY.
[15]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed: 18407956] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-546, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M98261 Genomic DNA. Translation: AAA34378.1. L22015 Genomic DNA. Translation: AAC04956.1. BK006935 Genomic DNA. Translation: DAA06985.1.
PIR	A46423.
RefSeq	NP_009400.1. NM_001178148.1.
3D structure databases
ProteinModelPortal	P34111.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-6739N.
IntAct	P34111. 3 interactions.
MINT	MINT-636763.
STRING	P34111.
Proteomic databases
PeptideAtlas	P34111.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YAL001C; YAL001C; YAL001C.
GeneID	851262.
KEGG	sce:YAL001C.
NMPDR	fig|4932.3.peg.84.
Organism-specific databases
CYGD	YAL001c.
SGD	S000000001. TFC3.
Phylogenomic databases
eggNOG	fuNOG07985.
GeneTree	EFGT00050000006536.
HOGENOM	HBG203637.
OMA	SSMIQRE.
OrthoDB	EOG45XC4B.
Enzyme and pathway databases
Reactome	REACT_87991. Transcription.
Gene expression databases
ArrayExpress	P34111.
Genevestigator	P34111.
GermOnline	YAL001C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR007309. TFIIIC_Bblock-bd. [Graphical view]
KO	K15204.
Pfam	PF04182. B-block_TFIIIC. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	968222.

Entry information

Entry name

TFC3_YEAST

Accession

Primary (citable) accession number: P34111
Secondary accession number(s): D6VPL5

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1994
Last sequence update:	February 1, 1994
Last modified:	December 14, 2011
This is version 97 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program