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P34111

- TFC3_YEAST

UniProt

P34111 - TFC3_YEAST

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Protein

Transcription factor tau 138 kDa subunit

Gene

TFC3

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

TFIIIC mediates tRNA and 5S RNA gene activation by binding to intragenic promoter elements. Upstream of the transcription start site, TFIIIC assembles the initiation complex TFIIIB-TFIIIC-tDNA, which is sufficient for RNA polymerase III recruitment and function. Part of the tauB domain of TFIIIC that binds boxB DNA promoter sites of tRNA and similar genes. TFC3 is essential for cell viability. Cooperates with TFC6 in DNA binding.3 Publications

GO - Molecular functioni

  1. DNA binding Source: UniProtKB-KW

GO - Biological processi

  1. 5S class rRNA transcription from RNA polymerase III type 1 promoter Source: SGD
  2. protein localization to chromatin Source: SGD
  3. regulation of transcription, DNA-templated Source: UniProtKB-KW
  4. RNA polymerase III type 1 promoter transcriptional preinitiation complex assembly Source: GOC
  5. RNA polymerase III type 2 promoter transcriptional preinitiation complex assembly Source: GOC
  6. transcription from a RNA polymerase III hybrid type promoter Source: SGD
  7. transcription from RNA polymerase III type 2 promoter Source: SGD
Complete GO annotation...

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-28816-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Transcription factor tau 138 kDa subunit
Alternative name(s):
TFIIIC 138 kDa subunit
Transcription factor C subunit 3
Gene namesi
Name:TFC3
Synonyms:TSV115
Ordered Locus Names:YAL001C
ORF Names:FUN24
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome I

Organism-specific databases

CYGDiYAL001c.
SGDiS000000001. TFC3.

Subcellular locationi

GO - Cellular componenti

  1. mitochondrion Source: UniProtKB-KW
  2. nucleoplasm Source: Reactome
  3. transcription factor TFIIIC complex Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi349 – 3491G → E in TSV115; thermosensitive. Level of TFIIIC and its affinity for tDNA reduced. tDNA binding activity very sensitive to mild heat treatments, and TFIIIC-DNA interaction inhibited at moderate salt concentrations. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 11601160Transcription factor tau 138 kDa subunitPRO_0000072497Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei546 – 5461Phosphoserine1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP34111.
PaxDbiP34111.
PeptideAtlasiP34111.

Expressioni

Gene expression databases

GenevestigatoriP34111.

Interactioni

Subunit structurei

Component of the TFIIIC complex composed of TFC1, TFC3, TFC4, TFC6, TFC7 and TFC8. The subunits are organized in two globular domains, tauA and tauB, connected by a proteolysis-sensitive and flexible linker. Interacts with TFC1, TFC4 and TFC6.4 Publications

Protein-protein interaction databases

BioGridi31789. 39 interactions.
DIPiDIP-6739N.
IntActiP34111. 3 interactions.
MINTiMINT-636763.
STRINGi4932.YAL001C.

Structurei

3D structure databases

ProteinModelPortaliP34111.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Phylogenomic databases

eggNOGiNOG72796.
HOGENOMiHOG000142056.
InParanoidiP34111.
KOiK15204.
OMAiMSSMIQR.
OrthoDBiEOG7RRFGW.

Family and domain databases

InterProiIPR007309. TFIIIC_Bblock-bd.
[Graphical view]
PfamiPF04182. B-block_TFIIIC. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P34111-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MVLTIYPDEL VQIVSDKIAS NKGKITLNQL WDISGKYFDL SDKKVKQFVL
60 70 80 90 100
SCVILKKDIE VYCDGAITTK NVTDIIGDAN HSYSVGITED SLWTLLTGYT
110 120 130 140 150
KKESTIGNSA FELLLEVAKS GEKGINTMDL AQVTGQDPRS VTGRIKKINH
160 170 180 190 200
LLTSSQLIYK GHVVKQLKLK KFSHDGVDSN PYINIRDHLA TIVEVVKRSK
210 220 230 240 250
NGIRQIIDLK RELKFDKEKR LSKAFIAAIA WLDEKEYLKK VLVVSPKNPA
260 270 280 290 300
IKIRCVKYVK DIPDSKGSPS FEYDSNSADE DSVSDSKAAF EDEDLVEGLD
310 320 330 340 350
NFNATDLLQN QGLVMEEKED AVKNEVLLNR FYPLQNQTYD IADKSGLKGI
360 370 380 390 400
STMDVVNRIT GKEFQRAFTK SSEYYLESVD KQKENTGGYR LFRIYDFEGK
410 420 430 440 450
KKFFRLFTAQ NFQKLTNAED EISVPKGFDE LGKSRTDLKT LNEDNFVALN
460 470 480 490 500
NTVRFTTDSD GQDIFFWHGE LKIPPNSKKT PNKNKRKRQV KNSTNASVAG
510 520 530 540 550
NISNPKRIKL EQHVSTAQEP KSAEDSPSSN GGTVVKGKVV NFGGFSARSL
560 570 580 590 600
RSLQRQRAIL KVMNTIGGVA YLREQFYESV SKYMGSTTTL DKKTVRGDVD
610 620 630 640 650
LMVESEKLGA RTEPVSGRKI IFLPTVGEDA IQRYILKEKD SKKATFTDVI
660 670 680 690 700
HDTEIYFFDQ TEKNRFHRGK KSVERIRKFQ NRQKNAKIKA SDDAISKKST
710 720 730 740 750
SVNVSDGKIK RRDKKVSAGR TTVVVENTKE DKTVYHAGTK DGVQALIRAV
760 770 780 790 800
VVTKSIKNEI MWDKITKLFP NNSLDNLKKK WTARRVRMGH SGWRAYVDKW
810 820 830 840 850
KKMLVLAIKS EKISLRDVEE LDLIKLLDIW TSFDEKEIKR PLFLYKNYEE
860 870 880 890 900
NRKKFTLVRD DTLTHSGNDL AMSSMIQREI SSLKKTYTRK ISASTKDLSK
910 920 930 940 950
SQSDDYIRTV IRSILIESPS TTRNEIEALK NVGNESIDNV IMDMAKEKQI
960 970 980 990 1000
YLHGSKLECT DTLPDILENR GNYKDFGVAF QYRCKVNELL EAGNAIVINQ
1010 1020 1030 1040 1050
EPSDISSWVL IDLISGELLN MDVIPMVRNV RPLTYTSRRF EIRTLTPPLI
1060 1070 1080 1090 1100
IYANSQTKLN TARKSAVKVP LGKPFSRLWV NGSGSIRPNI WKQVVTMVVN
1110 1120 1130 1140 1150
EIIFHPGITL SRLQSRCREV LSLHEISEIC KWLLERQVLI TTDFDGYWVN
1160
HNWYSIYEST
Length:1,160
Mass (Da):132,108
Last modified:February 1, 1994 - v1
Checksum:i3ACB7893ED536581
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M98261 Genomic DNA. Translation: AAA34378.1.
L22015 Genomic DNA. Translation: AAC04956.1.
BK006935 Genomic DNA. Translation: DAA06985.1.
PIRiA46423.
RefSeqiNP_009400.1. NM_001178148.1.

Genome annotation databases

EnsemblFungiiYAL001C; YAL001C; YAL001C.
GeneIDi851262.
KEGGisce:YAL001C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M98261 Genomic DNA. Translation: AAA34378.1 .
L22015 Genomic DNA. Translation: AAC04956.1 .
BK006935 Genomic DNA. Translation: DAA06985.1 .
PIRi A46423.
RefSeqi NP_009400.1. NM_001178148.1.

3D structure databases

ProteinModelPortali P34111.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 31789. 39 interactions.
DIPi DIP-6739N.
IntActi P34111. 3 interactions.
MINTi MINT-636763.
STRINGi 4932.YAL001C.

Proteomic databases

MaxQBi P34111.
PaxDbi P34111.
PeptideAtlasi P34111.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YAL001C ; YAL001C ; YAL001C .
GeneIDi 851262.
KEGGi sce:YAL001C.

Organism-specific databases

CYGDi YAL001c.
SGDi S000000001. TFC3.

Phylogenomic databases

eggNOGi NOG72796.
HOGENOMi HOG000142056.
InParanoidi P34111.
KOi K15204.
OMAi MSSMIQR.
OrthoDBi EOG7RRFGW.

Enzyme and pathway databases

BioCyci YEAST:G3O-28816-MONOMER.

Miscellaneous databases

NextBioi 968222.

Gene expression databases

Genevestigatori P34111.

Family and domain databases

InterProi IPR007309. TFIIIC_Bblock-bd.
[Graphical view ]
Pfami PF04182. B-block_TFIIIC. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "TFC3: gene encoding the B-block binding subunit of the yeast transcription factor IIIC."
    Lefebvre O., Carles C., Conesa C., Swanson R.N., Bouet F., Riva M., Sentenac A.
    Proc. Natl. Acad. Sci. U.S.A. 89:10512-10516(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 124-143; 331-347; 440-453; 508-520 AND 539-547, FUNCTION, IDENTIFICATION IN TFIIIC.
    Strain: ATCC 204508 / S288c.
  2. "A mutation in the largest subunit of yeast TFIIIC affects tRNA and 5 S RNA synthesis. Identification of two classes of suppressors."
    Lefebvre O., Rueth J., Sentenac A.
    J. Biol. Chem. 269:23374-23381(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], MUTAGENESIS OF GLY-349.
  3. "Sequencing of chromosome I of Saccharomyces cerevisiae: analysis of the 42 kbp SPO7-CENI-CDC15 region."
    Clark M.W., Keng T., Storms R.K., Zhong W.-W., Fortin N., Zeng B., Delaney S., Ouellette B.F.F., Barton A.B., Kaback D.B., Bussey H.
    Yeast 10:535-541(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204511 / S288c / AB972.
  4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  5. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  6. "Two polypeptide chains in yeast transcription factor tau interact with DNA."
    Gabrielsen O.S., Marzouki N., Ruet A., Sentenac A., Fromageot P.
    J. Biol. Chem. 264:7505-7511(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  7. "Purification and characterization of Saccharomyces cerevisiae transcription factor TFIIIC. Polypeptide composition defined with polyclonal antibodies."
    Parsons M.C., Weil P.A.
    J. Biol. Chem. 265:5095-5103(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION IN TFIIIC.
  8. "On the subunit composition, stoichiometry, and phosphorylation of the yeast transcription factor TFIIIC/tau."
    Conesa C., Swanson R.N., Schultz P., Oudet P., Sentenac A.
    J. Biol. Chem. 268:18047-18052(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH TFC1; TFC4 AND TFC6, PHOSPHORYLATION.
  9. "Tau91, an essential subunit of yeast transcription factor IIIC, cooperates with tau138 in DNA binding."
    Arrebola R., Manaud N., Rozenfeld S., Marsolier M.C., Lefebvre O., Carles C., Thuriaux P., Conesa C., Sentenac A.
    Mol. Cell. Biol. 18:1-9(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  10. "The tau95 subunit of yeast TFIIIC influences upstream and downstream functions of TFIIIC.DNA complexes."
    Jourdain S., Acker J., Ducrot C., Sentenac A., Lefebvre O.
    J. Biol. Chem. 278:10450-10457(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH TFC1 AND TFC6.
  11. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
  12. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  13. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
    Strain: ATCC 76625 / YPH499.
  14. "Toward the complete yeast mitochondrial proteome: multidimensional separation techniques for mitochondrial proteomics."
    Reinders J., Zahedi R.P., Pfanner N., Meisinger C., Sickmann A.
    J. Proteome Res. 5:1543-1554(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
  15. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
    Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
    Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-546, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiTFC3_YEAST
AccessioniPrimary (citable) accession number: P34111
Secondary accession number(s): D6VPL5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: February 1, 1994
Last modified: October 29, 2014
This is version 117 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 125 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  2. Yeast chromosome I
    Yeast (Saccharomyces cerevisiae) chromosome I: entries and gene names

External Data

Dasty 3