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P34111

- TFC3_YEAST

UniProt

P34111 - TFC3_YEAST

Protein

Transcription factor tau 138 kDa subunit

Gene

TFC3

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 116 (01 Oct 2014)
      Sequence version 1 (01 Feb 1994)
      Previous versions | rss
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    Functioni

    TFIIIC mediates tRNA and 5S RNA gene activation by binding to intragenic promoter elements. Upstream of the transcription start site, TFIIIC assembles the initiation complex TFIIIB-TFIIIC-tDNA, which is sufficient for RNA polymerase III recruitment and function. Part of the tauB domain of TFIIIC that binds boxB DNA promoter sites of tRNA and similar genes. TFC3 is essential for cell viability. Cooperates with TFC6 in DNA binding.3 Publications

    GO - Molecular functioni

    1. DNA binding Source: UniProtKB-KW

    GO - Biological processi

    1. 5S class rRNA transcription from RNA polymerase III type 1 promoter Source: SGD
    2. protein localization to chromatin Source: SGD
    3. regulation of transcription, DNA-templated Source: UniProtKB-KW
    4. RNA polymerase III type 1 promoter transcriptional preinitiation complex assembly Source: GOC
    5. RNA polymerase III type 2 promoter transcriptional preinitiation complex assembly Source: GOC
    6. transcription from a RNA polymerase III hybrid type promoter Source: SGD
    7. transcription from RNA polymerase III type 2 promoter Source: SGD

    Keywords - Biological processi

    Transcription, Transcription regulation

    Keywords - Ligandi

    DNA-binding

    Enzyme and pathway databases

    BioCyciYEAST:G3O-28816-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Transcription factor tau 138 kDa subunit
    Alternative name(s):
    TFIIIC 138 kDa subunit
    Transcription factor C subunit 3
    Gene namesi
    Name:TFC3
    Synonyms:TSV115
    Ordered Locus Names:YAL001C
    ORF Names:FUN24
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome I

    Organism-specific databases

    CYGDiYAL001c.
    SGDiS000000001. TFC3.

    Subcellular locationi

    GO - Cellular componenti

    1. mitochondrion Source: UniProtKB-SubCell
    2. nucleoplasm Source: Reactome
    3. transcription factor TFIIIC complex Source: SGD

    Keywords - Cellular componenti

    Mitochondrion, Nucleus

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi349 – 3491G → E in TSV115; thermosensitive. Level of TFIIIC and its affinity for tDNA reduced. tDNA binding activity very sensitive to mild heat treatments, and TFIIIC-DNA interaction inhibited at moderate salt concentrations. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 11601160Transcription factor tau 138 kDa subunitPRO_0000072497Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei546 – 5461Phosphoserine2 Publications

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiP34111.
    PaxDbiP34111.
    PeptideAtlasiP34111.

    Expressioni

    Gene expression databases

    GenevestigatoriP34111.

    Interactioni

    Subunit structurei

    Component of the TFIIIC complex composed of TFC1, TFC3, TFC4, TFC6, TFC7 and TFC8. The subunits are organized in two globular domains, tauA and tauB, connected by a proteolysis-sensitive and flexible linker. Interacts with TFC1, TFC4 and TFC6.4 Publications

    Protein-protein interaction databases

    BioGridi31789. 38 interactions.
    DIPiDIP-6739N.
    IntActiP34111. 3 interactions.
    MINTiMINT-636763.
    STRINGi4932.YAL001C.

    Structurei

    3D structure databases

    ProteinModelPortaliP34111.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Phylogenomic databases

    eggNOGiNOG72796.
    HOGENOMiHOG000142056.
    KOiK15204.
    OMAiMSSMIQR.
    OrthoDBiEOG7RRFGW.

    Family and domain databases

    InterProiIPR007309. TFIIIC_Bblock-bd.
    [Graphical view]
    PfamiPF04182. B-block_TFIIIC. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P34111-1 [UniParc]FASTAAdd to Basket

    « Hide

    MVLTIYPDEL VQIVSDKIAS NKGKITLNQL WDISGKYFDL SDKKVKQFVL     50
    SCVILKKDIE VYCDGAITTK NVTDIIGDAN HSYSVGITED SLWTLLTGYT 100
    KKESTIGNSA FELLLEVAKS GEKGINTMDL AQVTGQDPRS VTGRIKKINH 150
    LLTSSQLIYK GHVVKQLKLK KFSHDGVDSN PYINIRDHLA TIVEVVKRSK 200
    NGIRQIIDLK RELKFDKEKR LSKAFIAAIA WLDEKEYLKK VLVVSPKNPA 250
    IKIRCVKYVK DIPDSKGSPS FEYDSNSADE DSVSDSKAAF EDEDLVEGLD 300
    NFNATDLLQN QGLVMEEKED AVKNEVLLNR FYPLQNQTYD IADKSGLKGI 350
    STMDVVNRIT GKEFQRAFTK SSEYYLESVD KQKENTGGYR LFRIYDFEGK 400
    KKFFRLFTAQ NFQKLTNAED EISVPKGFDE LGKSRTDLKT LNEDNFVALN 450
    NTVRFTTDSD GQDIFFWHGE LKIPPNSKKT PNKNKRKRQV KNSTNASVAG 500
    NISNPKRIKL EQHVSTAQEP KSAEDSPSSN GGTVVKGKVV NFGGFSARSL 550
    RSLQRQRAIL KVMNTIGGVA YLREQFYESV SKYMGSTTTL DKKTVRGDVD 600
    LMVESEKLGA RTEPVSGRKI IFLPTVGEDA IQRYILKEKD SKKATFTDVI 650
    HDTEIYFFDQ TEKNRFHRGK KSVERIRKFQ NRQKNAKIKA SDDAISKKST 700
    SVNVSDGKIK RRDKKVSAGR TTVVVENTKE DKTVYHAGTK DGVQALIRAV 750
    VVTKSIKNEI MWDKITKLFP NNSLDNLKKK WTARRVRMGH SGWRAYVDKW 800
    KKMLVLAIKS EKISLRDVEE LDLIKLLDIW TSFDEKEIKR PLFLYKNYEE 850
    NRKKFTLVRD DTLTHSGNDL AMSSMIQREI SSLKKTYTRK ISASTKDLSK 900
    SQSDDYIRTV IRSILIESPS TTRNEIEALK NVGNESIDNV IMDMAKEKQI 950
    YLHGSKLECT DTLPDILENR GNYKDFGVAF QYRCKVNELL EAGNAIVINQ 1000
    EPSDISSWVL IDLISGELLN MDVIPMVRNV RPLTYTSRRF EIRTLTPPLI 1050
    IYANSQTKLN TARKSAVKVP LGKPFSRLWV NGSGSIRPNI WKQVVTMVVN 1100
    EIIFHPGITL SRLQSRCREV LSLHEISEIC KWLLERQVLI TTDFDGYWVN 1150
    HNWYSIYEST 1160
    Length:1,160
    Mass (Da):132,108
    Last modified:February 1, 1994 - v1
    Checksum:i3ACB7893ED536581
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M98261 Genomic DNA. Translation: AAA34378.1.
    L22015 Genomic DNA. Translation: AAC04956.1.
    BK006935 Genomic DNA. Translation: DAA06985.1.
    PIRiA46423.
    RefSeqiNP_009400.1. NM_001178148.1.

    Genome annotation databases

    EnsemblFungiiYAL001C; YAL001C; YAL001C.
    GeneIDi851262.
    KEGGisce:YAL001C.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M98261 Genomic DNA. Translation: AAA34378.1 .
    L22015 Genomic DNA. Translation: AAC04956.1 .
    BK006935 Genomic DNA. Translation: DAA06985.1 .
    PIRi A46423.
    RefSeqi NP_009400.1. NM_001178148.1.

    3D structure databases

    ProteinModelPortali P34111.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 31789. 38 interactions.
    DIPi DIP-6739N.
    IntActi P34111. 3 interactions.
    MINTi MINT-636763.
    STRINGi 4932.YAL001C.

    Proteomic databases

    MaxQBi P34111.
    PaxDbi P34111.
    PeptideAtlasi P34111.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YAL001C ; YAL001C ; YAL001C .
    GeneIDi 851262.
    KEGGi sce:YAL001C.

    Organism-specific databases

    CYGDi YAL001c.
    SGDi S000000001. TFC3.

    Phylogenomic databases

    eggNOGi NOG72796.
    HOGENOMi HOG000142056.
    KOi K15204.
    OMAi MSSMIQR.
    OrthoDBi EOG7RRFGW.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-28816-MONOMER.

    Miscellaneous databases

    NextBioi 968222.

    Gene expression databases

    Genevestigatori P34111.

    Family and domain databases

    InterProi IPR007309. TFIIIC_Bblock-bd.
    [Graphical view ]
    Pfami PF04182. B-block_TFIIIC. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "TFC3: gene encoding the B-block binding subunit of the yeast transcription factor IIIC."
      Lefebvre O., Carles C., Conesa C., Swanson R.N., Bouet F., Riva M., Sentenac A.
      Proc. Natl. Acad. Sci. U.S.A. 89:10512-10516(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 124-143; 331-347; 440-453; 508-520 AND 539-547, FUNCTION, IDENTIFICATION IN TFIIIC.
      Strain: ATCC 204508 / S288c.
    2. "A mutation in the largest subunit of yeast TFIIIC affects tRNA and 5 S RNA synthesis. Identification of two classes of suppressors."
      Lefebvre O., Rueth J., Sentenac A.
      J. Biol. Chem. 269:23374-23381(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], MUTAGENESIS OF GLY-349.
    3. "Sequencing of chromosome I of Saccharomyces cerevisiae: analysis of the 42 kbp SPO7-CENI-CDC15 region."
      Clark M.W., Keng T., Storms R.K., Zhong W.-W., Fortin N., Zeng B., Delaney S., Ouellette B.F.F., Barton A.B., Kaback D.B., Bussey H.
      Yeast 10:535-541(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 204511 / S288c / AB972.
    4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    5. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    6. "Two polypeptide chains in yeast transcription factor tau interact with DNA."
      Gabrielsen O.S., Marzouki N., Ruet A., Sentenac A., Fromageot P.
      J. Biol. Chem. 264:7505-7511(1989) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    7. "Purification and characterization of Saccharomyces cerevisiae transcription factor TFIIIC. Polypeptide composition defined with polyclonal antibodies."
      Parsons M.C., Weil P.A.
      J. Biol. Chem. 265:5095-5103(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION IN TFIIIC.
    8. "On the subunit composition, stoichiometry, and phosphorylation of the yeast transcription factor TFIIIC/tau."
      Conesa C., Swanson R.N., Schultz P., Oudet P., Sentenac A.
      J. Biol. Chem. 268:18047-18052(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH TFC1; TFC4 AND TFC6, PHOSPHORYLATION.
    9. "Tau91, an essential subunit of yeast transcription factor IIIC, cooperates with tau138 in DNA binding."
      Arrebola R., Manaud N., Rozenfeld S., Marsolier M.C., Lefebvre O., Carles C., Thuriaux P., Conesa C., Sentenac A.
      Mol. Cell. Biol. 18:1-9(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    10. "The tau95 subunit of yeast TFIIIC influences upstream and downstream functions of TFIIIC.DNA complexes."
      Jourdain S., Acker J., Ducrot C., Sentenac A., Lefebvre O.
      J. Biol. Chem. 278:10450-10457(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH TFC1 AND TFC6.
    11. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
    12. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    13. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
      Strain: ATCC 76625 / YPH499.
    14. "Toward the complete yeast mitochondrial proteome: multidimensional separation techniques for mitochondrial proteomics."
      Reinders J., Zahedi R.P., Pfanner N., Meisinger C., Sickmann A.
      J. Proteome Res. 5:1543-1554(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
    15. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
      Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
      Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-546, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiTFC3_YEAST
    AccessioniPrimary (citable) accession number: P34111
    Secondary accession number(s): D6VPL5
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1994
    Last sequence update: February 1, 1994
    Last modified: October 1, 2014
    This is version 116 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 125 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    2. Yeast chromosome I
      Yeast (Saccharomyces cerevisiae) chromosome I: entries and gene names

    External Data

    Dasty 3