Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Vacuolar protein sorting-associated protein 35

Gene

VPS35

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Plays a role in vesicular protein sorting. Required for the endosome-to-Golgi retrieval of the vacuolar protein sorting receptor VPS10. Vacuolar carboxypeptidase Y (CPY) sorting is completely dependent on the presence of functional VPS35. The latter may play a role in the function, modification or packaging of a CPY-specific receptor complex. Component of the membrane-associated retromer complex which is essential in endosome-to-Golgi retrograde transport. The VPS29-VPS26-VPS35 subcomplex may be involved in cargo selection.

Miscellaneous

Present with 2360 molecules/cell in log phase SD medium.1 Publication

GO - Molecular functioni

GO - Biological processi

  • intracellular protein transport Source: GO_Central
  • negative regulation of cell death Source: ParkinsonsUK-UCL
  • negative regulation of endoplasmic reticulum unfolded protein response Source: ParkinsonsUK-UCL
  • positive regulation of protein localization to cell periphery Source: ParkinsonsUK-UCL
  • protein retention in Golgi apparatus Source: SGD
  • retrograde transport, endosome to Golgi Source: SGD

Keywordsi

Biological processProtein transport, Transport

Enzyme and pathway databases

BioCyciYEAST:G3O-31595-MONOMER.
ReactomeiR-SCE-3238698. WNT ligand biogenesis and trafficking.

Protein family/group databases

TCDBi9.A.63.1.1. the retromer-dependent vacuolar protein sorting (r-vps) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Vacuolar protein sorting-associated protein 35
Alternative name(s):
Vacuolar protein-targeting protein 7
Gene namesi
Name:VPS35
Synonyms:VPT7
Ordered Locus Names:YJL154C
ORF Names:J0580
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome X

Organism-specific databases

EuPathDBiFungiDB:YJL154C.
SGDiS000003690. VPS35.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000658991 – 944Vacuolar protein sorting-associated protein 35Add BLAST944

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei846PhosphoserineCombined sources1
Modified residuei848PhosphoserineCombined sources1
Modified residuei868PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP34110.
PRIDEiP34110.

PTM databases

iPTMnetiP34110.

Interactioni

Subunit structurei

Component of the retromer complex which consists of VPS29, VPS26, VPS35, VPS5 and VPS17. Component of a retromer subcomplex consisting of VPS29, VPS26 and VPS35.1 Publication

Binary interactionsi

Show more details

Protein-protein interaction databases

BioGridi33606. 350 interactors.
DIPiDIP-1743N.
IntActiP34110. 17 interactors.
MINTiMINT-384284.
STRINGi4932.YJL154C.

Structurei

3D structure databases

ProteinModelPortaliP34110.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the VPS35 family.Curated

Phylogenomic databases

GeneTreeiENSGT00390000007315.
HOGENOMiHOG000196946.
InParanoidiP34110.
KOiK18468.
OMAiRVADSCM.
OrthoDBiEOG092C0TIO.

Family and domain databases

InterProiView protein in InterPro
IPR005378. Vps35.
PANTHERiPTHR11099. PTHR11099. 1 hit.
PfamiView protein in Pfam
PF03635. Vps35. 1 hit.
PIRSFiPIRSF009375. Retromer_Vps35. 1 hit.

Sequencei

Sequence statusi: Complete.

P34110-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAYADSPENA IAVIKQRTAL MNRCLSQHKL MESLQHTSIM LTELRNPNLS
60 70 80 90 100
PKKYYELYVI IFDSLTNLST YLIENHPQNH HLADLYELVQ YTGNVVPRLY
110 120 130 140 150
LMITVGTSYL TFNEAPKKEI LKDMIEMCRG VQNPIRGLFL RYYLSQRTKE
160 170 180 190 200
LLPEDDPSFN SQFIMNNFIE MNKLWVRLQH QGPLRERETR TRERKELQIL
210 220 230 240 250
VGSQLVRLSQ IIDDNFQMYK QDILPTILEQ VIQCRDLVSQ EYLLDVICQV
260 270 280 290 300
FADEFHLKTL DTLLQTTLHL NPDVSINKIV LTLVDRLNDY VTRQLEDDPN
310 320 330 340 350
ATSTNAYLDM DVFGTFWDYL TVLNHERPDL SLQQFIPLVE SVIVLSLKWY
360 370 380 390 400
PNNFDNLNKL FELVLQKTKD YGQKNISLES EHLFLVLLSF QNSKLQLTSS
410 420 430 440 450
TTAPPNSPVT SKKHFIFQLI SQCQAYKNIL ALQSISLQKK VVNEIIDILM
460 470 480 490 500
DREVEEMADN DSESKLHPPG HSAYLVIEDK LQVQRLLSIC EPLIISRSGP
510 520 530 540 550
PANVASSDTN VDEVFFNRHD EEESWILDPI QEKLAHLIHW IMNTTSRKQT
560 570 580 590 600
MKNKIQFSLE AQLEILLLIK SSFIKGGINV KYTFPAIITN FWKLMRKCRM
610 620 630 640 650
IQEYLLKKRP DNKTLLSHYS NLLKQMFKFV SRCINDIFNS CNNSCTDLIL
660 670 680 690 700
KLNLQCAILA DQLQLNEISY DFFSQAFTIF EESLSDSKTQ LQALIYIAQS
710 720 730 740 750
LQKTRSLYKE AYYDSLIVRC TLHGSKLLKK QDQCRAVYLC SHLWWATEIS
760 770 780 790 800
NIGEEEGITD NFYRDGKRVL ECLQRSLRVA DSIMDNEQSC ELMVEILNRC
810 820 830 840 850
LYYFIHGDES ETHISIKYIN GLIELIKTNL KSLKLEDNSA SMITNSISDL
860 870 880 890 900
HITGENNVKA SSNADDGSVI TDKESNVAIG SDGTYIQLNT LNGSSTLIRG
910 920 930 940
VVATASGSKL LHQLKYIPIH HFRRTCEYIE SQREVDDRFK VIYV
Length:944
Mass (Da):109,142
Last modified:November 1, 1995 - v2
Checksum:iDE1BC57B9DFB606E
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti14 – 20Missing in AAB22844 (PubMed:1498362).Curated7
Sequence conflicti661D → E in AAB22844 (PubMed:1498362).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
S42186 Genomic DNA. Translation: AAB22844.1.
Z49429 Genomic DNA. Translation: CAA89449.1.
X87371 Genomic DNA. Translation: CAA60801.1.
BK006943 Genomic DNA. Translation: DAA08649.1.
PIRiS56936.
RefSeqiNP_012381.1. NM_001181587.1.

Genome annotation databases

EnsemblFungiiYJL154C; YJL154C; YJL154C.
GeneIDi853287.
KEGGisce:YJL154C.

Similar proteinsi

Entry informationi

Entry nameiVPS35_YEAST
AccessioniPrimary (citable) accession number: P34110
Secondary accession number(s): D6VW33
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: November 1, 1995
Last modified: November 22, 2017
This is version 159 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome X
    Yeast (Saccharomyces cerevisiae) chromosome X: entries and gene names