P34110 (VPS35_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Vacuolar protein sorting-associated protein 35

Alternative name(s):
Vacuolar protein-targeting protein 7

Gene names

Name:	VPS35
Synonyms:	VPT7
Ordered Locus Names:	YJL154C
ORF Names:	J0580

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	944 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Plays a role in vesicular protein sorting. Required for the endosome-to-Golgi retrieval of the vacuolar protein sorting receptor VPS10. Vacuolar carboxypeptidase Y (CPY) sorting is completely dependent on the presence of functional VPS35. The latter may play a role in the function, modification or packaging of a CPY-specific receptor complex. Component of the membrane-associated retromer complex which is essential in endosome-to-Golgi retrograde transport. The VPS29-VPS26-VPS35 subcomplex may be involved in cargo selection.
Subunit structure	Component of the retromer complex which consists of VPS29, VPS26, VPS35, VPS5 and VPS17. Component of a retromer subcomplex consisting of VPS29, VPS26 and VPS35.
Subcellular location	Membrane; Peripheral membrane protein.
Miscellaneous	Present with 2360 molecules/cell in log phase SD medium. Ref.6
Sequence similarities	Belongs to the VPS35 family.

Ontologies

Keywords
Biological process	Protein transport Transport
Cellular component	Membrane
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	protein retention in Golgi apparatus Inferred from mutant phenotype. Source: SGD protein transport Inferred from physical interaction. Source: GOC retrograde transport, endosome to Golgi Inferred from physical interaction Ref.5. Source: SGD
Cellular component	endosome Inferred from physical interaction Ref.5. Source: SGD retromer complex, inner shell Inferred from physical interaction Ref.5. Source: SGD
Molecular function	protein binding Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
VPS17	P32913	3	EBI-20415,EBI-20366
VPS29	P38759	3	EBI-20415,EBI-13092
VPS5	Q92331	3	EBI-20415,EBI-20483

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 944	944	Vacuolar protein sorting-associated protein 35		PRO_0000065899
Amino acid modifications
	Modified residue	507	1	Phosphoserine Ref.7
	Modified residue	844	1	Phosphothreonine Ref.7
	Modified residue	868	1	Phosphoserine Ref.7
Experimental info
	Sequence conflict	14 – 20	7	Missing in AAB22844. Ref.1
	Sequence conflict	661	1	D → E in AAB22844. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P34110 [UniParc]. Last modified November 1, 1995. Version 2. Checksum: DE1BC57B9DFB606E	FASTA	944	109,142
	10 20 30 40 50 60 MAYADSPENA IAVIKQRTAL MNRCLSQHKL MESLQHTSIM LTELRNPNLS PKKYYELYVI 70 80 90 100 110 120 IFDSLTNLST YLIENHPQNH HLADLYELVQ YTGNVVPRLY LMITVGTSYL TFNEAPKKEI 130 140 150 160 170 180 LKDMIEMCRG VQNPIRGLFL RYYLSQRTKE LLPEDDPSFN SQFIMNNFIE MNKLWVRLQH 190 200 210 220 230 240 QGPLRERETR TRERKELQIL VGSQLVRLSQ IIDDNFQMYK QDILPTILEQ VIQCRDLVSQ 250 260 270 280 290 300 EYLLDVICQV FADEFHLKTL DTLLQTTLHL NPDVSINKIV LTLVDRLNDY VTRQLEDDPN 310 320 330 340 350 360 ATSTNAYLDM DVFGTFWDYL TVLNHERPDL SLQQFIPLVE SVIVLSLKWY PNNFDNLNKL 370 380 390 400 410 420 FELVLQKTKD YGQKNISLES EHLFLVLLSF QNSKLQLTSS TTAPPNSPVT SKKHFIFQLI 430 440 450 460 470 480 SQCQAYKNIL ALQSISLQKK VVNEIIDILM DREVEEMADN DSESKLHPPG HSAYLVIEDK 490 500 510 520 530 540 LQVQRLLSIC EPLIISRSGP PANVASSDTN VDEVFFNRHD EEESWILDPI QEKLAHLIHW 550 560 570 580 590 600 IMNTTSRKQT MKNKIQFSLE AQLEILLLIK SSFIKGGINV KYTFPAIITN FWKLMRKCRM 610 620 630 640 650 660 IQEYLLKKRP DNKTLLSHYS NLLKQMFKFV SRCINDIFNS CNNSCTDLIL KLNLQCAILA 670 680 690 700 710 720 DQLQLNEISY DFFSQAFTIF EESLSDSKTQ LQALIYIAQS LQKTRSLYKE AYYDSLIVRC 730 740 750 760 770 780 TLHGSKLLKK QDQCRAVYLC SHLWWATEIS NIGEEEGITD NFYRDGKRVL ECLQRSLRVA 790 800 810 820 830 840 DSIMDNEQSC ELMVEILNRC LYYFIHGDES ETHISIKYIN GLIELIKTNL KSLKLEDNSA 850 860 870 880 890 900 SMITNSISDL HITGENNVKA SSNADDGSVI TDKESNVAIG SDGTYIQLNT LNGSSTLIRG 910 920 930 940 VVATASGSKL LHQLKYIPIH HFRRTCEYIE SQREVDDRFK VIYV

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Alternative pathways for the sorting of soluble vacuolar proteins in yeast: a vps35 null mutant missorts and secretes only a subset of vacuolar hydrolases." Paravicini G., Horazdovsky B.F., Emr S.D. Mol. Biol. Cell 3:415-427(1992) [PubMed: 1498362] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Complete nucleotide sequence of Saccharomyces cerevisiae chromosome X." Galibert F., Alexandraki D., Baur A., Boles E., Chalwatzis N., Chuat J.-C., Coster F., Cziepluch C., de Haan M., Domdey H., Durand P., Entian K.-D., Gatius M., Goffeau A., Grivell L.A., Hennemann A., Herbert C.J., Heumann K. , Hilger F., Hollenberg C.P., Huang M.-E., Jacq C., Jauniaux J.-C., Katsoulou C., Kirchrath L., Kleine K., Kordes E., Koetter P., Liebl S., Louis E.J., Manus V., Mewes H.-W., Miosga T., Obermaier B., Perea J., Pohl T.M., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rasmussen S.W., Rose M., Rossau R., Schaaff-Gerstenschlaeger I., Smits P.H.M., Scarcez T., Soriano N., To Van D., Tzermia M., Van Broekhoven A., Vandenbol M., Wedler H., von Wettstein D., Wambutt R., Zagulski M., Zollner A., Karpfinger-Hartl L. EMBO J. 15:2031-2049(1996) [PubMed: 8641269] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[3]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[4]	"Sequence analysis of a 40.7 kb segment from the left arm of yeast chromosome X reveals 14 known genes and 13 new open reading frames including homologues of genes clustered on the right arm of chromosome XI." Katsoulou C., Tzermia M., Tavernarakis N., Alexandraki D. Yeast 12:787-797(1996) [PubMed: 8813765] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-937. Strain: ATCC 96604 / S288c / FY1679.
[5]	"A membrane coat complex essential for endosome-to-Golgi retrograde transport in yeast." Seaman M.N., McCaffery J.M., Emr S.D. J. Cell Biol. 142:665-681(1998) [PubMed: 9700157] [Abstract] Cited for: IDENTIFICATION IN THE RETROMER COMPLEX.
[6]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[7]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed: 18407956] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-507; THR-844 AND SER-868, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	S42186 Genomic DNA. Translation: AAB22844.1. Z49429 Genomic DNA. Translation: CAA89449.1. X87371 Genomic DNA. Translation: CAA60801.1. BK006943 Genomic DNA. Translation: DAA08649.1.
PIR	S56936.
RefSeq	NP_012381.1. NM_001181587.1.
3D structure databases
ProteinModelPortal	P34110.
SMR	P34110. Positions 526-838.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-1743N.
IntAct	P34110. 20 interactions.
MINT	MINT-384284.
STRING	P34110.
Proteomic databases
PeptideAtlas	P34110.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YJL154C; YJL154C; YJL154C.
GeneID	853287.
KEGG	sce:YJL154C.
NMPDR	fig|4932.3.peg.3346.
Organism-specific databases
CYGD	YJL154c.
SGD	S000003690. VPS35.
Phylogenomic databases
eggNOG	fuNOG04566.
GeneTree	EFGT00050000006701.
HOGENOM	HBG317989.
OMA	ISQEYLM.
OrthoDB	EOG4M68RP.
Gene expression databases
ArrayExpress	P34110.
Genevestigator	P34110.
GermOnline	YJL154C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR005378. VPS35. [Graphical view]
PANTHER	PTHR11099. Vps35. 1 hit.
Pfam	PF03635. Vps35. 1 hit. [Graphical view]
PIRSF	PIRSF009375. Retromer_Vps35. 1 hit.
ProtoNet	Search...
Other
NextBio	973588.

Entry information

Entry name

VPS35_YEAST

Accession

Primary (citable) accession number: P34110
Secondary accession number(s): D6VW33

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1994
Last sequence update:	November 1, 1995
Last modified:	January 25, 2012
This is version 103 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program