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Protein

Vacuolar protein sorting-associated protein 35

Gene

VPS35

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Plays a role in vesicular protein sorting. Required for the endosome-to-Golgi retrieval of the vacuolar protein sorting receptor VPS10. Vacuolar carboxypeptidase Y (CPY) sorting is completely dependent on the presence of functional VPS35. The latter may play a role in the function, modification or packaging of a CPY-specific receptor complex. Component of the membrane-associated retromer complex which is essential in endosome-to-Golgi retrograde transport. The VPS29-VPS26-VPS35 subcomplex may be involved in cargo selection.

GO - Molecular functioni

GO - Biological processi

  • intracellular protein transport Source: GO_Central
  • protein retention in Golgi apparatus Source: SGD
  • retrograde transport, endosome to Golgi Source: SGD
Complete GO annotation...

Keywords - Biological processi

Protein transport, Transport

Enzyme and pathway databases

BioCyciYEAST:G3O-31595-MONOMER.
ReactomeiR-SCE-3238698. WNT ligand biogenesis and trafficking.

Protein family/group databases

TCDBi9.A.63.1.1. the retromer-dependnet vacuolar protein sorting (r-vps) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Vacuolar protein sorting-associated protein 35
Alternative name(s):
Vacuolar protein-targeting protein 7
Gene namesi
Name:VPS35
Synonyms:VPT7
Ordered Locus Names:YJL154C
ORF Names:J0580
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome X

Organism-specific databases

EuPathDBiFungiDB:YJL154C.
SGDiS000003690. VPS35.

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: GOC
  • endosome Source: SGD
  • fungal-type vacuole membrane Source: SGD
  • late endosome Source: GO_Central
  • retromer, cargo-selective complex Source: SGD
  • retromer complex Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 944944Vacuolar protein sorting-associated protein 35PRO_0000065899Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei846 – 8461PhosphoserineCombined sources
Modified residuei848 – 8481PhosphoserineCombined sources
Modified residuei868 – 8681PhosphoserineCombined sources

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP34110.

PTM databases

iPTMnetiP34110.

Interactioni

Subunit structurei

Component of the retromer complex which consists of VPS29, VPS26, VPS35, VPS5 and VPS17. Component of a retromer subcomplex consisting of VPS29, VPS26 and VPS35.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
VPS17P329133EBI-20415,EBI-20366
VPS29P387593EBI-20415,EBI-13092
VPS5Q923313EBI-20415,EBI-20483

Protein-protein interaction databases

BioGridi33606. 144 interactions.
DIPiDIP-1743N.
IntActiP34110. 16 interactions.
MINTiMINT-384284.

Structurei

3D structure databases

ProteinModelPortaliP34110.
SMRiP34110. Positions 560-842.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the VPS35 family.Curated

Phylogenomic databases

GeneTreeiENSGT00390000007315.
HOGENOMiHOG000196946.
InParanoidiP34110.
KOiK18468.
OMAiLDVICQV.
OrthoDBiEOG092C0TIO.

Family and domain databases

InterProiIPR005378. Vps35.
[Graphical view]
PANTHERiPTHR11099. PTHR11099. 4 hits.
PfamiPF03635. Vps35. 1 hit.
[Graphical view]
PIRSFiPIRSF009375. Retromer_Vps35. 1 hit.

Sequencei

Sequence statusi: Complete.

P34110-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAYADSPENA IAVIKQRTAL MNRCLSQHKL MESLQHTSIM LTELRNPNLS
60 70 80 90 100
PKKYYELYVI IFDSLTNLST YLIENHPQNH HLADLYELVQ YTGNVVPRLY
110 120 130 140 150
LMITVGTSYL TFNEAPKKEI LKDMIEMCRG VQNPIRGLFL RYYLSQRTKE
160 170 180 190 200
LLPEDDPSFN SQFIMNNFIE MNKLWVRLQH QGPLRERETR TRERKELQIL
210 220 230 240 250
VGSQLVRLSQ IIDDNFQMYK QDILPTILEQ VIQCRDLVSQ EYLLDVICQV
260 270 280 290 300
FADEFHLKTL DTLLQTTLHL NPDVSINKIV LTLVDRLNDY VTRQLEDDPN
310 320 330 340 350
ATSTNAYLDM DVFGTFWDYL TVLNHERPDL SLQQFIPLVE SVIVLSLKWY
360 370 380 390 400
PNNFDNLNKL FELVLQKTKD YGQKNISLES EHLFLVLLSF QNSKLQLTSS
410 420 430 440 450
TTAPPNSPVT SKKHFIFQLI SQCQAYKNIL ALQSISLQKK VVNEIIDILM
460 470 480 490 500
DREVEEMADN DSESKLHPPG HSAYLVIEDK LQVQRLLSIC EPLIISRSGP
510 520 530 540 550
PANVASSDTN VDEVFFNRHD EEESWILDPI QEKLAHLIHW IMNTTSRKQT
560 570 580 590 600
MKNKIQFSLE AQLEILLLIK SSFIKGGINV KYTFPAIITN FWKLMRKCRM
610 620 630 640 650
IQEYLLKKRP DNKTLLSHYS NLLKQMFKFV SRCINDIFNS CNNSCTDLIL
660 670 680 690 700
KLNLQCAILA DQLQLNEISY DFFSQAFTIF EESLSDSKTQ LQALIYIAQS
710 720 730 740 750
LQKTRSLYKE AYYDSLIVRC TLHGSKLLKK QDQCRAVYLC SHLWWATEIS
760 770 780 790 800
NIGEEEGITD NFYRDGKRVL ECLQRSLRVA DSIMDNEQSC ELMVEILNRC
810 820 830 840 850
LYYFIHGDES ETHISIKYIN GLIELIKTNL KSLKLEDNSA SMITNSISDL
860 870 880 890 900
HITGENNVKA SSNADDGSVI TDKESNVAIG SDGTYIQLNT LNGSSTLIRG
910 920 930 940
VVATASGSKL LHQLKYIPIH HFRRTCEYIE SQREVDDRFK VIYV
Length:944
Mass (Da):109,142
Last modified:November 1, 1995 - v2
Checksum:iDE1BC57B9DFB606E
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti14 – 207Missing in AAB22844 (PubMed:1498362).Curated
Sequence conflicti661 – 6611D → E in AAB22844 (PubMed:1498362).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
S42186 Genomic DNA. Translation: AAB22844.1.
Z49429 Genomic DNA. Translation: CAA89449.1.
X87371 Genomic DNA. Translation: CAA60801.1.
BK006943 Genomic DNA. Translation: DAA08649.1.
PIRiS56936.
RefSeqiNP_012381.1. NM_001181587.1.

Genome annotation databases

EnsemblFungiiYJL154C; YJL154C; YJL154C.
GeneIDi853287.
KEGGisce:YJL154C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
S42186 Genomic DNA. Translation: AAB22844.1.
Z49429 Genomic DNA. Translation: CAA89449.1.
X87371 Genomic DNA. Translation: CAA60801.1.
BK006943 Genomic DNA. Translation: DAA08649.1.
PIRiS56936.
RefSeqiNP_012381.1. NM_001181587.1.

3D structure databases

ProteinModelPortaliP34110.
SMRiP34110. Positions 560-842.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi33606. 144 interactions.
DIPiDIP-1743N.
IntActiP34110. 16 interactions.
MINTiMINT-384284.

Protein family/group databases

TCDBi9.A.63.1.1. the retromer-dependnet vacuolar protein sorting (r-vps) family.

PTM databases

iPTMnetiP34110.

Proteomic databases

MaxQBiP34110.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYJL154C; YJL154C; YJL154C.
GeneIDi853287.
KEGGisce:YJL154C.

Organism-specific databases

EuPathDBiFungiDB:YJL154C.
SGDiS000003690. VPS35.

Phylogenomic databases

GeneTreeiENSGT00390000007315.
HOGENOMiHOG000196946.
InParanoidiP34110.
KOiK18468.
OMAiLDVICQV.
OrthoDBiEOG092C0TIO.

Enzyme and pathway databases

BioCyciYEAST:G3O-31595-MONOMER.
ReactomeiR-SCE-3238698. WNT ligand biogenesis and trafficking.

Miscellaneous databases

PROiP34110.

Family and domain databases

InterProiIPR005378. Vps35.
[Graphical view]
PANTHERiPTHR11099. PTHR11099. 4 hits.
PfamiPF03635. Vps35. 1 hit.
[Graphical view]
PIRSFiPIRSF009375. Retromer_Vps35. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiVPS35_YEAST
AccessioniPrimary (citable) accession number: P34110
Secondary accession number(s): D6VW33
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: November 1, 1995
Last modified: September 7, 2016
This is version 147 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 2360 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome X
    Yeast (Saccharomyces cerevisiae) chromosome X: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.