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P34106 (ALA2_PANMI) Reviewed, UniProtKB/Swiss-Prot

Last modified October 16, 2013. Version 70. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Alanine aminotransferase 2

Short name=ALAAT-2
EC=2.6.1.2
Alternative name(s):
Glutamate pyruvate transaminase 2
Short name=GPT
Glutamic--alanine transaminase 2
Glutamic--pyruvic transaminase 2
OrganismPanicum miliaceum (Proso millet) (Broomcorn millet)
Taxonomic identifier4540 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaePACMAD cladePanicoideaePaniceaePanicum

Protein attributes

Sequence length482 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Transfer of C3 units between the cytosol of mesophyll and bundle sheath cells to maintain a nitrogen-carbon balance in the C4-dicarboxylic pathway.

Catalytic activity

L-alanine + 2-oxoglutarate = pyruvate + L-glutamate.

Cofactor

Pyridoxal phosphate.

Pathway

Photosynthesis; C4 acid pathway.

Amino-acid degradation; L-alanine degradation via transaminase pathway; pyruvate from L-alanine: step 1/1.

Subunit structure

Homodimer By similarity.

Tissue specificity

Mesophyll and bundle sheath cells.

Induction

By light.

Post-translational modification

The N-terminus is blocked.

Sequence similarities

Belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family. Alanine aminotransferase subfamily.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 482482Alanine aminotransferase 2
PRO_0000123940

Amino acid modifications

Modified residue2991N6-(pyridoxal phosphate)lysine By similarity

Sequences

Sequence LengthMass (Da)Tools
P34106 [UniParc].

Last modified February 1, 1994. Version 1.
Checksum: FB6562233A0D3E21

FASTA48252,682
        10         20         30         40         50         60 
MAATVAVENL NPKVLKCEYA VRGEIVIHAQ HLQQQLQTQP GSLPFDEILY CNIGNPQSLG 

        70         80         90        100        110        120 
QQPVTFFREV LALCDHPCLL EKEETKSLFS ADAISRAKQI LSTIPGRATG AYSHSQGIKG 

       130        140        150        160        170        180 
LRDAIAAGIA SRDGFPANAD DIFVTDGASP GVHMMMQLLI RNEKDGILCP IPQYPLYSAS 

       190        200        210        220        230        240 
IALHGGTLVP YYLDEKTGWG LEISDLKKQL EDARSKGIDV RALVVINPGN PTGQVLAEDN 

       250        260        270        280        290        300 
QCDIVRFCKN EGLVLLADEV YQENIYVDDK KFNSFKKIAR SVGYGEDDLP LVSFQSVSKG 

       310        320        330        340        350        360 
YYGECGKRGG YMEITGFSAP VREQIYKIAS VNLCSNITGQ ILASLVMNPP KVGDESYAAY 

       370        380        390        400        410        420 
KAEKDGILQS LARRAKALED AFNNLEGISC NKAEGAMYLF PQIHLPKKAI EAAKAANKAP 

       430        440        450        460        470        480 
DAFYALRLLE STGIVVVPGS GFGQVPGTWH IRCTILPQED KIPAVITRFK AFHEAFMAEY 


RD 

« Hide

References

[1]"Molecular cloning of an alanine aminotransferase from NAD-malic enzyme type C4 plant Panicum miliaceum."
Son D., Sugiyama T.
Plant Mol. Biol. 20:705-713(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 197-206 AND 308-317.
Tissue: Leaf.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X69421 mRNA. Translation: CAA49199.1.
PIRS28429.

3D structure databases

ProteinModelPortalP34106.
ModBaseSearch...
MobiDBSearch...

Proteomic databases

PRIDEP34106.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Organism-specific databases

GrameneP34106.

Enzyme and pathway databases

BioCycMetaCyc:MONOMER-17674.
UniPathwayUPA00322.
UPA00528; UER00586.

Family and domain databases

Gene3D3.40.640.10. 1 hit.
3.90.1150.10. 1 hit.
InterProIPR004839. Aminotransferase_I/II.
IPR015424. PyrdxlP-dep_Trfase.
IPR015421. PyrdxlP-dep_Trfase_major_sub1.
IPR015422. PyrdxlP-dep_Trfase_major_sub2.
[Graphical view]
PfamPF00155. Aminotran_1_2. 1 hit.
[Graphical view]
SUPFAMSSF53383. SSF53383. 1 hit.
ProtoNetSearch...

Entry information

Entry nameALA2_PANMI
AccessionPrimary (citable) accession number: P34106
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: February 1, 1994
Last modified: October 16, 2013
This is version 70 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways