P34106 (ALA2_PANMI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names	Recommended name: Alanine aminotransferase 2 Short name=ALAAT-2 EC=2.6.1.2 Alternative name(s): Glutamate pyruvate transaminase 2 Short name=GPT Glutamic--alanine transaminase 2 Glutamic--pyruvic transaminase 2
Organism	Panicum miliaceum (Proso millet) (Broomcorn millet)
Taxonomic identifier	4540 [NCBI]
Taxonomic lineage	cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › Liliopsida › commelinids › Poales › Poaceae › PACMAD clade › Panicoideae › Paniceae › Panicum

Protein attributes

Sequence length	482 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Transfer of C3 units between the cytosol of mesophyll and bundle sheath cells to maintain a nitrogen-carbon balance in the C4-dicarboxylic pathway.
Catalytic activity	L-alanine + 2-oxoglutarate = pyruvate + L-glutamate.
Cofactor	Pyridoxal phosphate.
Pathway	Photosynthesis; C4 acid pathway. Amino-acid degradation; L-alanine degradation via transaminase pathway; pyruvate from L-alanine: step 1/1.
Subunit structure	Homodimer By similarity.
Tissue specificity	Mesophyll and bundle sheath cells.
Induction	By light.
Post-translational modification	The N-terminus is blocked.
Sequence similarities	Belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family. Alanine aminotransferase subfamily.

Ontologies

Keywords
Ligand	Pyridoxal phosphate
Molecular function	Aminotransferase Transferase
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological_process	L-alanine catabolic process Inferred from electronic annotation. Source: UniProtKB-UniPathway biosynthetic process Inferred from electronic annotation. Source: InterPro
Molecular_function	L-alanine:2-oxoglutarate aminotransferase activity Inferred from electronic annotation. Source: EC pyridoxal phosphate binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 482	482	Alanine aminotransferase 2		PRO_0000123940
Amino acid modifications
	Modified residue	299	1	N6-(pyridoxal phosphate)lysine By similarity

Sequences

	Sequence		Length	Mass (Da)
	P34106 [UniParc]. Last modified February 1, 1994. Version 1. Checksum: FB6562233A0D3E21	FASTA	482	52,682
	10 20 30 40 50 60 MAATVAVENL NPKVLKCEYA VRGEIVIHAQ HLQQQLQTQP GSLPFDEILY CNIGNPQSLG 70 80 90 100 110 120 QQPVTFFREV LALCDHPCLL EKEETKSLFS ADAISRAKQI LSTIPGRATG AYSHSQGIKG 130 140 150 160 170 180 LRDAIAAGIA SRDGFPANAD DIFVTDGASP GVHMMMQLLI RNEKDGILCP IPQYPLYSAS 190 200 210 220 230 240 IALHGGTLVP YYLDEKTGWG LEISDLKKQL EDARSKGIDV RALVVINPGN PTGQVLAEDN 250 260 270 280 290 300 QCDIVRFCKN EGLVLLADEV YQENIYVDDK KFNSFKKIAR SVGYGEDDLP LVSFQSVSKG 310 320 330 340 350 360 YYGECGKRGG YMEITGFSAP VREQIYKIAS VNLCSNITGQ ILASLVMNPP KVGDESYAAY 370 380 390 400 410 420 KAEKDGILQS LARRAKALED AFNNLEGISC NKAEGAMYLF PQIHLPKKAI EAAKAANKAP 430 440 450 460 470 480 DAFYALRLLE STGIVVVPGS GFGQVPGTWH IRCTILPQED KIPAVITRFK AFHEAFMAEY RD

References

[1]	"Molecular cloning of an alanine aminotransferase from NAD-malic enzyme type C4 plant Panicum miliaceum." Son D., Sugiyama T. Plant Mol. Biol. 20:705-713(1992) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 197-206 AND 308-317. Tissue: Leaf.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X69421 mRNA. Translation: CAA49199.1.
PIR	S28429.
3D structure databases
ProteinModelPortal	P34106.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
KEGG	dosa:Os07t0617800-01. dosa:Os10t0390600-01.
Organism-specific databases
Gramene	P34106.
Enzyme and pathway databases
BioCyc	MetaCyc:MONOMER-17674.
UniPathway	UPA00322. UPA00528; UER00586.
Family and domain databases
Gene3D	3.40.640.10. 1 hit. 3.90.1150.10. 1 hit.
InterPro	IPR004839. Aminotransferase_I/II. IPR015424. PyrdxlP-dep_Trfase. IPR015421. PyrdxlP-dep_Trfase_major_sub1. IPR015422. PyrdxlP-dep_Trfase_major_sub2. [Graphical view]
Pfam	PF00155. Aminotran_1_2. 1 hit. [Graphical view]
SUPFAM	SSF53383. PyrdxlP-dep_Trfase_major. 1 hit.
ProtoNet	Search...

Entry information

Entry name

ALA2_PANMI

Accession

Primary (citable) accession number: P34106

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1994
Last sequence update:	February 1, 1994
Last modified:	April 3, 2013
This is version 68 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program