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P34106

- ALA2_PANMI

UniProt

P34106 - ALA2_PANMI

Protein

Alanine aminotransferase 2

Gene
N/A
Organism
Panicum miliaceum (Proso millet) (Broomcorn millet)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 71 (01 Oct 2014)
      Sequence version 1 (01 Feb 1994)
      Previous versions | rss
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    • Comment

    Functioni

    Transfer of C3 units between the cytosol of mesophyll and bundle sheath cells to maintain a nitrogen-carbon balance in the C4-dicarboxylic pathway.

    Catalytic activityi

    L-alanine + 2-oxoglutarate = pyruvate + L-glutamate.

    Cofactori

    Pyridoxal phosphate.

    Pathwayi

    GO - Molecular functioni

    1. L-alanine:2-oxoglutarate aminotransferase activity Source: UniProtKB-EC
    2. pyridoxal phosphate binding Source: InterPro

    GO - Biological processi

    1. biosynthetic process Source: InterPro
    2. L-alanine catabolic process Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Aminotransferase, Transferase

    Keywords - Ligandi

    Pyridoxal phosphate

    Enzyme and pathway databases

    BioCyciMetaCyc:MONOMER-17674.
    UniPathwayiUPA00322.
    UPA00528; UER00586.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Alanine aminotransferase 2 (EC:2.6.1.2)
    Short name:
    ALAAT-2
    Alternative name(s):
    Glutamate pyruvate transaminase 2
    Short name:
    GPT
    Glutamic--alanine transaminase 2
    Glutamic--pyruvic transaminase 2
    OrganismiPanicum miliaceum (Proso millet) (Broomcorn millet)
    Taxonomic identifieri4540 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaePACMAD cladePanicoideaePaniceaePanicum

    Organism-specific databases

    GrameneiP34106.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 482482Alanine aminotransferase 2PRO_0000123940Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei299 – 2991N6-(pyridoxal phosphate)lysineBy similarity

    Post-translational modificationi

    The N-terminus is blocked.

    Proteomic databases

    PRIDEiP34106.

    Expressioni

    Tissue specificityi

    Mesophyll and bundle sheath cells.

    Inductioni

    By light.

    Interactioni

    Subunit structurei

    Homodimer.By similarity

    Structurei

    3D structure databases

    ProteinModelPortaliP34106.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Family and domain databases

    Gene3Di3.40.640.10. 1 hit.
    3.90.1150.10. 1 hit.
    InterProiIPR004839. Aminotransferase_I/II.
    IPR015424. PyrdxlP-dep_Trfase.
    IPR015421. PyrdxlP-dep_Trfase_major_sub1.
    IPR015422. PyrdxlP-dep_Trfase_major_sub2.
    [Graphical view]
    PfamiPF00155. Aminotran_1_2. 1 hit.
    [Graphical view]
    SUPFAMiSSF53383. SSF53383. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    P34106-1 [UniParc]FASTAAdd to Basket

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    MAATVAVENL NPKVLKCEYA VRGEIVIHAQ HLQQQLQTQP GSLPFDEILY    50
    CNIGNPQSLG QQPVTFFREV LALCDHPCLL EKEETKSLFS ADAISRAKQI 100
    LSTIPGRATG AYSHSQGIKG LRDAIAAGIA SRDGFPANAD DIFVTDGASP 150
    GVHMMMQLLI RNEKDGILCP IPQYPLYSAS IALHGGTLVP YYLDEKTGWG 200
    LEISDLKKQL EDARSKGIDV RALVVINPGN PTGQVLAEDN QCDIVRFCKN 250
    EGLVLLADEV YQENIYVDDK KFNSFKKIAR SVGYGEDDLP LVSFQSVSKG 300
    YYGECGKRGG YMEITGFSAP VREQIYKIAS VNLCSNITGQ ILASLVMNPP 350
    KVGDESYAAY KAEKDGILQS LARRAKALED AFNNLEGISC NKAEGAMYLF 400
    PQIHLPKKAI EAAKAANKAP DAFYALRLLE STGIVVVPGS GFGQVPGTWH 450
    IRCTILPQED KIPAVITRFK AFHEAFMAEY RD 482
    Length:482
    Mass (Da):52,682
    Last modified:February 1, 1994 - v1
    Checksum:iFB6562233A0D3E21
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X69421 mRNA. Translation: CAA49199.1.
    PIRiS28429.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X69421 mRNA. Translation: CAA49199.1 .
    PIRi S28429.

    3D structure databases

    ProteinModelPortali P34106.
    ModBasei Search...
    MobiDBi Search...

    Proteomic databases

    PRIDEi P34106.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Organism-specific databases

    Gramenei P34106.

    Enzyme and pathway databases

    UniPathwayi UPA00322 .
    UPA00528 ; UER00586 .
    BioCyci MetaCyc:MONOMER-17674.

    Family and domain databases

    Gene3Di 3.40.640.10. 1 hit.
    3.90.1150.10. 1 hit.
    InterProi IPR004839. Aminotransferase_I/II.
    IPR015424. PyrdxlP-dep_Trfase.
    IPR015421. PyrdxlP-dep_Trfase_major_sub1.
    IPR015422. PyrdxlP-dep_Trfase_major_sub2.
    [Graphical view ]
    Pfami PF00155. Aminotran_1_2. 1 hit.
    [Graphical view ]
    SUPFAMi SSF53383. SSF53383. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Molecular cloning of an alanine aminotransferase from NAD-malic enzyme type C4 plant Panicum miliaceum."
      Son D., Sugiyama T.
      Plant Mol. Biol. 20:705-713(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 197-206 AND 308-317.
      Tissue: Leaf.

    Entry informationi

    Entry nameiALA2_PANMI
    AccessioniPrimary (citable) accession number: P34106
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1994
    Last sequence update: February 1, 1994
    Last modified: October 1, 2014
    This is version 71 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Direct protein sequencing

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3